AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37812

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37812.t1 MTR_6g079410 74.249 466 82 6 1 431 1 463 0.0 681
MS.gene37812.t1 MTR_6g463380 53.864 427 125 8 42 431 134 525 1.19e-140 413
MS.gene37812.t1 MTR_3g006860 54.098 427 124 8 42 431 130 521 1.29e-139 410
MS.gene37812.t1 MTR_6g463380 55.728 323 106 5 42 329 134 454 7.39e-114 342
MS.gene37812.t1 MTR_6g463380 55.763 321 105 5 42 327 134 452 1.06e-112 338
MS.gene37812.t1 MTR_6g061650 43.966 348 126 10 98 432 22 313 4.25e-70 224
MS.gene37812.t1 MTR_1g014390 34.494 316 112 6 123 432 63 289 5.47e-45 158
MS.gene37812.t1 MTR_6g006850 32.919 322 162 8 119 432 27 302 2.14e-41 149
MS.gene37812.t1 MTR_6g006710 30.435 299 131 7 136 431 63 287 1.21e-30 120
MS.gene37812.t1 MTR_1g029560 36.275 204 93 5 231 431 27 196 1.06e-28 112
MS.gene37812.t1 MTR_1g012010 33.645 214 96 3 123 329 66 240 1.02e-27 110
MS.gene37812.t1 MTR_6g080120 85.106 47 7 0 328 374 1 47 7.10e-22 89.0
MS.gene37812.t1 MTR_7g025575 41.748 103 32 3 328 429 13 88 2.99e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37812.t1 AT5G51740 42.918 473 189 15 1 432 1 433 1.37e-107 325
MS.gene37812.t1 AT5G51740 42.918 473 189 15 1 432 44 476 3.74e-107 325
MS.gene37812.t1 AT5G51740 42.350 366 166 11 1 327 44 403 4.25e-83 261

Find 71 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCGTGTGTTTACAAAATA+TGG 0.134991 6.3:-73533872 MS.gene37812:CDS
TGGACTGAATTGGGAGATTT+TGG 0.167567 6.3:-73533506 MS.gene37812:CDS
GAGACTGAGCGGAGTGATTT+AGG 0.187812 6.3:-73533652 MS.gene37812:CDS
AATGTTTCCCTTGTTCTATT+TGG 0.195503 6.3:-73533919 MS.gene37812:CDS
CCAAAGGTGGTCCTTATAAT+AGG 0.266161 6.3:-73534071 MS.gene37812:CDS
ATAGCGAGATCGGGAAATTC+TGG 0.277309 6.3:+73534107 None:intergenic
CTTGTTCTATTTGGAGTTGT+TGG 0.286663 6.3:-73533910 MS.gene37812:CDS
GCAACCTTATACGTCGCATT+TGG 0.332750 6.3:-73533530 MS.gene37812:CDS
CCATCCAAATGCGACGTATA+AGG 0.337352 6.3:+73533526 None:intergenic
CGCATTTGGATGGACTGAAT+TGG 0.338297 6.3:-73533516 MS.gene37812:CDS
TGCCTTCAACTTCAACTTCA+AGG 0.342590 6.3:-73534164 MS.gene37812:CDS
CTATTTGGAGTTGTTGGTTC+AGG 0.346705 6.3:-73533904 MS.gene37812:CDS
ATGAGAAACTTGATGACAAA+TGG 0.355330 6.3:-73533579 MS.gene37812:CDS
CTCTGAGAAAACCTATTATA+AGG 0.371133 6.3:+73534060 None:intergenic
ACACTTTGAGGGTGTGTTGC+CGG 0.386983 6.3:+73533733 None:intergenic
AAGGAAAAGTTGAAGGGAAT+TGG 0.390236 6.3:-73533612 MS.gene37812:CDS
AGACTGAGCGGAGTGATTTA+GGG 0.391676 6.3:-73533651 MS.gene37812:CDS
TGCCAAGAGGGGCAAAAGAT+TGG 0.400180 6.3:-73533957 MS.gene37812:CDS
CTGAGCTTTAGCGAGCGATT+CGG 0.424071 6.3:+73531535 None:intergenic
GATCGGGAAATTCTGGAGTT+AGG 0.430128 6.3:+73534114 None:intergenic
AAACTAGAATCACGTGTGTT+CGG 0.433419 6.3:+73533830 None:intergenic
GCATTTGGATGGACTGAATT+GGG 0.433979 6.3:-73533515 MS.gene37812:CDS
ACCCTCAAAGTGTGAGAGTT+GGG 0.436760 6.3:-73533723 MS.gene37812:CDS
ACGTGTGTTCGGTTTGTGTA+AGG 0.437622 6.3:+73533841 None:intergenic
CGAAATTAAAATAGCGAGAT+CGG 0.438044 6.3:+73534097 None:intergenic
GAGAATGAAGGAAAAGTTGA+AGG 0.438255 6.3:-73533619 MS.gene37812:CDS
TGAATTGCTCTTTCAAGCTC+AGG 0.442207 6.3:-73531570 MS.gene37812:CDS
ATCCCAACTCTCACACTTTG+AGG 0.443157 6.3:+73533721 None:intergenic
CCTATTATAAGGACCACCTT+TGG 0.446812 6.3:+73534071 None:intergenic
TTTGGCGACCTTTCGGAGAA+AGG 0.449893 6.3:-73532942 MS.gene37812:intron
TTCAGAATTCACTCATGGCT+TGG 0.450144 6.3:-73534230 None:intergenic
AAACTGCTCAGCACTGTGTC+TGG 0.465687 6.3:+73533032 None:intergenic
GCTCGCTAAAGCTCAGGTAA+TGG 0.469980 6.3:-73531528 MS.gene37812:CDS
GGGAAGATAGTTGTATTCAC+TGG 0.478426 6.3:-73533442 MS.gene37812:CDS
CCTTATACGTCGCATTTGGA+TGG 0.481840 6.3:-73533526 MS.gene37812:CDS
GAGCGGAGTGATTTAGGGTA+TGG 0.481841 6.3:-73533646 MS.gene37812:CDS
AAACAAAAGGCATCAACAAA+AGG 0.483071 6.3:+73533472 None:intergenic
GAAATTAAAATAGCGAGATC+GGG 0.493255 6.3:+73534098 None:intergenic
GCTTCAGCTTTAGATATATG+AGG 0.496330 6.3:+73531647 None:intergenic
TGAGAAACTTGATGACAAAT+GGG 0.499318 6.3:-73533578 MS.gene37812:CDS
GTCTCCAAGCTTCTTCTCCA+TGG 0.500499 6.3:+73533801 None:intergenic
TCCCAACTCTCACACTTTGA+GGG 0.505571 6.3:+73533722 None:intergenic
AGCCTTGAAGTTGAAGTTGA+AGG 0.510117 6.3:+73534162 None:intergenic
CGAATCGCTCGCTAAAGCTC+AGG 0.514358 6.3:-73531534 MS.gene37812:CDS
TGATCCTCATGTGGCTCCTA+CGG 0.519441 6.3:-73531732 MS.gene37812:CDS
TTTAATTTCGCTACCAAAGG+TGG 0.527691 6.3:-73534084 MS.gene37812:CDS
CTTCTATTAGTCGCATCAGC+AGG 0.528066 6.3:-73531758 MS.gene37812:CDS
AATGCCATGGAGAAGAAGCT+TGG 0.530715 6.3:-73533805 MS.gene37812:CDS
TGATGACAAATGGGTTCAGA+AGG 0.531380 6.3:-73533569 MS.gene37812:CDS
GGGTATGGTTCAGAGAATGA+AGG 0.538268 6.3:-73533631 MS.gene37812:CDS
CTTCCCACCAGGTAAACAAA+AGG 0.543528 6.3:+73533459 None:intergenic
CTGCGTGTTTGAAAAGATGA+AGG 0.567504 6.3:-73533779 MS.gene37812:CDS
CAACAACTCCAAATAGAACA+AGG 0.568302 6.3:+73533911 None:intergenic
AGAATGAAGGAAAAGTTGAA+GGG 0.571867 6.3:-73533618 MS.gene37812:CDS
CACCCTCAAAGTGTGAGAGT+TGG 0.577769 6.3:-73533724 MS.gene37812:CDS
GATTCTAGTTTCGAATGCCA+TGG 0.579969 6.3:-73533818 MS.gene37812:CDS
AGTTGAAGGGAATTGGCAGA+TGG 0.582954 6.3:-73533605 MS.gene37812:CDS
TCTCAGAGAATAGGAACAAG+TGG 0.583572 6.3:-73534045 MS.gene37812:CDS
AATCATTACCTTTCTCCGAA+AGG 0.593435 6.3:+73532934 None:intergenic
ATGAATTTCTCTAAGACCGT+AGG 0.593712 6.3:+73531716 None:intergenic
AGCAGTTTACACATAATCAG+TGG 0.621012 6.3:-73533017 MS.gene37812:CDS
TATATCTAAAGCTGAAGCCG+AGG 0.624435 6.3:-73531642 MS.gene37812:CDS
CAAATGGGTTCAGAAGGGTA+AGG 0.643159 6.3:-73533563 MS.gene37812:CDS
GATGACAAATGGGTTCAGAA+GGG 0.648502 6.3:-73533568 MS.gene37812:CDS
AACAACTCCAAATAGAACAA+GGG 0.652012 6.3:+73533912 None:intergenic
AATACAACTATCTTCCCACC+AGG 0.662092 6.3:+73533448 None:intergenic
AGCAGGCTATGATCCTCATG+TGG 0.663662 6.3:-73531741 MS.gene37812:CDS
GATGAAGAAAACATTGAACA+AGG 0.665144 6.3:-73533674 MS.gene37812:CDS
TGTATTGCATGCTCATGCTG+AGG 0.685341 6.3:-73531606 MS.gene37812:CDS
AAGACCGTAGGAGCCACATG+AGG 0.724893 6.3:+73531728 None:intergenic
CATTGAACAAGGAGACTGAG+CGG 0.761321 6.3:-73533663 MS.gene37812:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGAAAATTGTATAATTTT+AGG - chr6.3:73533802-73533821 MS.gene37812:CDS 10.0%
!!! TTGAAATTAATAACAATTTT+AGG - chr6.3:73531686-73531705 MS.gene37812:CDS 10.0%
!! ATTGTTATAAAGATAAACAA+TGG + chr6.3:73532801-73532820 None:intergenic 15.0%
!!! TTTTTAACCAAATTATACTA+AGG - chr6.3:73533389-73533408 MS.gene37812:CDS 15.0%
!! AAAAAAGTAAACTTAAAACG+CGG - chr6.3:73533093-73533112 MS.gene37812:intron 20.0%
!! AAATTATACTAAGGATAACT+AGG - chr6.3:73533398-73533417 MS.gene37812:CDS 20.0%
!! CAAATCTAGTATTAGAAATT+AGG - chr6.3:73533449-73533468 MS.gene37812:CDS 20.0%
!! CTAATTTCTAATACTAGATT+TGG + chr6.3:73533451-73533470 None:intergenic 20.0%
!! TAGTTATCCTTAGTATAATT+TGG + chr6.3:73533399-73533418 None:intergenic 20.0%
!! TATGAATGATTAAATGTTCT+CGG - chr6.3:73533181-73533200 MS.gene37812:intron 20.0%
!!! TGATCTAAAATTATAGGAAT+AGG - chr6.3:73533735-73533754 MS.gene37812:CDS 20.0%
! AAAAAGAAGCTGATTACATT+GGG - chr6.3:73533925-73533944 MS.gene37812:CDS 25.0%
! AAAATTTATTGACGACATTC+AGG - chr6.3:73533898-73533917 MS.gene37812:CDS 25.0%
! AGTCTATTTGAGAGATATTA+TGG - chr6.3:73532416-73532435 MS.gene37812:intron 25.0%
! ATAATCATTCATTTATGCGA+AGG + chr6.3:73533152-73533171 None:intergenic 25.0%
! GAAGTGAAAAACATGAAATT+AGG - chr6.3:73532919-73532938 MS.gene37812:intron 25.0%
! GTTATAAAGATAAACAATGG+AGG + chr6.3:73532798-73532817 None:intergenic 25.0%
! TGAAAAATAGAGTTATGAGA+TGG + chr6.3:73531571-73531590 None:intergenic 25.0%
!! AACCAACTTTTATAGAGATT+TGG - chr6.3:73533329-73533348 MS.gene37812:intron 25.0%
!! ATAAAATGCCACACATTTTT+AGG + chr6.3:73531730-73531749 None:intergenic 25.0%
!! ATAAGTAATGTTTGTTAGAC+AGG - chr6.3:73532858-73532877 MS.gene37812:intron 25.0%
!! ATAATTTTAGATCATGTCAC+AGG + chr6.3:73533729-73533748 None:intergenic 25.0%
!! GGTCAAAGACTAATAAATTT+TGG - chr6.3:73532940-73532959 MS.gene37812:intron 25.0%
!! GTGACATGATCTAAAATTAT+AGG - chr6.3:73533729-73533748 MS.gene37812:CDS 25.0%
!! TGTATAATTTTAGGCGTTTT+AGG - chr6.3:73533811-73533830 MS.gene37812:CDS 25.0%
!! TTCCATATTTTGTAAACACA+CGG + chr6.3:73531839-73531858 None:intergenic 25.0%
!! TTCTAATACTAGATTTGGTT+CGG + chr6.3:73533446-73533465 None:intergenic 25.0%
!!! TATTTTAATTTCGCTACCAA+AGG - chr6.3:73531619-73531638 MS.gene37812:CDS 25.0%
!!! TCTCTAGAATAATTATCGTT+CGG - chr6.3:73533237-73533256 MS.gene37812:intron 25.0%
AACAACTCCAAATAGAACAA+GGG + chr6.3:73531797-73531816 None:intergenic 30.0%
AATGTTTCCCTTGTTCTATT+TGG - chr6.3:73531787-73531806 MS.gene37812:CDS 30.0%
AGAATGAAGGAAAAGTTGAA+GGG - chr6.3:73532088-73532107 MS.gene37812:intron 30.0%
CAACCCTTGATTTAAAGAAA+GGG + chr6.3:73533119-73533138 None:intergenic 30.0%
CACCAAATCTCTATAAAAGT+TGG + chr6.3:73533334-73533353 None:intergenic 30.0%
CGAAATTAAAATAGCGAGAT+CGG + chr6.3:73531612-73531631 None:intergenic 30.0%
CTATTTCAACCAAGAAATGT+GGG + chr6.3:73533786-73533805 None:intergenic 30.0%
GAAAAAGAAGCTGATTACAT+TGG - chr6.3:73533924-73533943 MS.gene37812:CDS 30.0%
GAAATTAAAATAGCGAGATC+GGG + chr6.3:73531611-73531630 None:intergenic 30.0%
GATGAAGAAAACATTGAACA+AGG - chr6.3:73532032-73532051 MS.gene37812:intron 30.0%
GCAAAAAAAATGACCTTGTT+TGG + chr6.3:73532584-73532603 None:intergenic 30.0%
TAAATTGAACTAAACCGAAC+TGG - chr6.3:73533527-73533546 MS.gene37812:CDS 30.0%
TACCGTGTGTTTACAAAATA+TGG - chr6.3:73531834-73531853 MS.gene37812:intron 30.0%
TCTATTTCAACCAAGAAATG+TGG + chr6.3:73533787-73533806 None:intergenic 30.0%
TGGTTAAAAAAATCCCAGTT+TGG + chr6.3:73533379-73533398 None:intergenic 30.0%
TGGTTTAGAAACTACAGAAA+TGG + chr6.3:73533359-73533378 None:intergenic 30.0%
TGTAGTTTCTAAACCAAACT+GGG - chr6.3:73533363-73533382 MS.gene37812:intron 30.0%
TTGAGCTATTTATCCAAACA+AGG - chr6.3:73532568-73532587 MS.gene37812:intron 30.0%
TTTGTTAGACAGGTTAGAAT+AGG - chr6.3:73532868-73532887 MS.gene37812:intron 30.0%
! AATAGGTTTTCTCAGAGAAT+AGG - chr6.3:73531652-73531671 MS.gene37812:CDS 30.0%
! AATTAGTGTTACCACGATTT+TGG - chr6.3:73532966-73532985 MS.gene37812:CDS 30.0%
! AGAACAAGGGAAACATTTTT+AGG + chr6.3:73531784-73531803 None:intergenic 30.0%
! AGTTCAATTTTTCTGAACTG+TGG + chr6.3:73533287-73533306 None:intergenic 30.0%
! ATGAGAAACTTGATGACAAA+TGG - chr6.3:73532127-73532146 MS.gene37812:intron 30.0%
! ATGCAATATTGATTGTGGAA+AGG - chr6.3:73533834-73533853 MS.gene37812:CDS 30.0%
! CGATAATTATTCTAGAGAGT+AGG + chr6.3:73533235-73533254 None:intergenic 30.0%
! CTCTGAGAAAACCTATTATA+AGG + chr6.3:73531649-73531668 None:intergenic 30.0%
! CTTTAAAAATGAGTTGTTGC+CGG - chr6.3:73531954-73531973 MS.gene37812:intron 30.0%
! TGAGAAACTTGATGACAAAT+GGG - chr6.3:73532128-73532147 MS.gene37812:intron 30.0%
! TTAGGATGCAATATTGATTG+TGG - chr6.3:73533829-73533848 MS.gene37812:CDS 30.0%
!! AAACAAAAGGCATCAACAAA+AGG + chr6.3:73532237-73532256 None:intergenic 30.0%
AAACTAGAATCACGTGTGTT+CGG + chr6.3:73531879-73531898 None:intergenic 35.0%
AAATCTAACACTTTGCTGCT+TGG - chr6.3:73532628-73532647 MS.gene37812:intron 35.0%
AAGGAAAAGTTGAAGGGAAT+TGG - chr6.3:73532094-73532113 MS.gene37812:intron 35.0%
AATCATTACCTTTCTCCGAA+AGG + chr6.3:73532775-73532794 None:intergenic 35.0%
AGCAGTTTACACATAATCAG+TGG - chr6.3:73532689-73532708 MS.gene37812:intron 35.0%
ATTCGCTCTTTATGTCTGTT+TGG - chr6.3:73532353-73532372 MS.gene37812:intron 35.0%
CAACAACTCCAAATAGAACA+AGG + chr6.3:73531798-73531817 None:intergenic 35.0%
CTGTAGTTTCTAAACCAAAC+TGG - chr6.3:73533362-73533381 MS.gene37812:intron 35.0%
CTGTGTATCCTAAAAATGTG+TGG - chr6.3:73531719-73531738 MS.gene37812:CDS 35.0%
GAATAATTATCGTTCGGTTG+AGG - chr6.3:73533243-73533262 MS.gene37812:intron 35.0%
GAGAATGAAGGAAAAGTTGA+AGG - chr6.3:73532087-73532106 MS.gene37812:intron 35.0%
GCAACCCTTGATTTAAAGAA+AGG + chr6.3:73533120-73533139 None:intergenic 35.0%
GTAATTAAGCCCACATTTCT+TGG - chr6.3:73533774-73533793 MS.gene37812:CDS 35.0%
TGAACTATACGAAACCAGTT+CGG + chr6.3:73533544-73533563 None:intergenic 35.0%
TTTATTGACGACATTCAGGA+TGG - chr6.3:73533902-73533921 MS.gene37812:CDS 35.0%
! CTTGTTCTATTTGGAGTTGT+TGG - chr6.3:73531796-73531815 MS.gene37812:intron 35.0%
! GCAGAGATAGCAACTTTTAT+CGG - chr6.3:73532312-73532331 MS.gene37812:intron 35.0%
! TACAGCTCTATATTTTGCCT+CGG + chr6.3:73534084-73534103 None:intergenic 35.0%
! TATTGATTGTGGAAAGGTGA+AGG - chr6.3:73533840-73533859 MS.gene37812:CDS 35.0%
! TTTAATTTCGCTACCAAAGG+TGG - chr6.3:73531622-73531641 MS.gene37812:CDS 35.0%
!! ATGAATTTCTCTAAGACCGT+AGG + chr6.3:73533993-73534012 None:intergenic 35.0%
!! GACTGTCATCACTTTTCTTT+TGG - chr6.3:73532746-73532765 MS.gene37812:intron 35.0%
!! GCTTCAGCTTTAGATATATG+AGG + chr6.3:73534062-73534081 None:intergenic 35.0%
!! TCTCTATAAAAGTTGGTTCG+AGG + chr6.3:73533327-73533346 None:intergenic 35.0%
AACTAGTGAAACGTATGAGC+TGG - chr6.3:73533671-73533690 MS.gene37812:CDS 40.0%
AATACAACTATCTTCCCACC+AGG + chr6.3:73532261-73532280 None:intergenic 40.0%
AGCCTTGAAGTTGAAGTTGA+AGG + chr6.3:73531547-73531566 None:intergenic 40.0%
ATGTCACAGGAACAATGCTA+GGG + chr6.3:73533716-73533735 None:intergenic 40.0%
CCAAAGGTGGTCCTTATAAT+AGG - chr6.3:73531635-73531654 MS.gene37812:CDS 40.0%
CCTATTATAAGGACCACCTT+TGG + chr6.3:73531638-73531657 None:intergenic 40.0%
CGGCCCTTTCTTTAAATCAA+GGG - chr6.3:73533113-73533132 MS.gene37812:intron 40.0%
CTAGTTCATAACAATGCTGC+AGG + chr6.3:73533657-73533676 None:intergenic 40.0%
CTGCGTGTTTGAAAAGATGA+AGG - chr6.3:73531927-73531946 MS.gene37812:intron 40.0%
GGGAAGATAGTTGTATTCAC+TGG - chr6.3:73532264-73532283 MS.gene37812:intron 40.0%
TCTAGAGAGTAGGTACTACT+AGG + chr6.3:73533225-73533244 None:intergenic 40.0%
TCTCAGAGAATAGGAACAAG+TGG - chr6.3:73531661-73531680 MS.gene37812:CDS 40.0%
TCTGAACTGTGGTAACAGTT+CGG + chr6.3:73533276-73533295 None:intergenic 40.0%
TGAATTGCTCTTTCAAGCTC+AGG - chr6.3:73534136-73534155 MS.gene37812:CDS 40.0%
TGCAGTTCAGTTCAGCAATT+TGG + chr6.3:73533496-73533515 None:intergenic 40.0%
TGCCTTCAACTTCAACTTCA+AGG - chr6.3:73531542-73531561 MS.gene37812:CDS 40.0%
TGTGGGCTTAATTACACAAG+TGG + chr6.3:73533769-73533788 None:intergenic 40.0%
! ACTGAATTGGGAGATTTTGG+TGG - chr6.3:73532203-73532222 MS.gene37812:intron 40.0%
! CACTTTGCTGCTTGGTAATT+AGG - chr6.3:73532636-73532655 MS.gene37812:intron 40.0%
! CTTTTCTTTTGGCGACCTTT+CGG - chr6.3:73532757-73532776 MS.gene37812:intron 40.0%
! GATGACAAATGGGTTCAGAA+GGG - chr6.3:73532138-73532157 MS.gene37812:intron 40.0%
! TATATCTAAAGCTGAAGCCG+AGG - chr6.3:73534064-73534083 MS.gene37812:CDS 40.0%
! TGATGACAAATGGGTTCAGA+AGG - chr6.3:73532137-73532156 MS.gene37812:intron 40.0%
! TGGACTGAATTGGGAGATTT+TGG - chr6.3:73532200-73532219 MS.gene37812:intron 40.0%
! TGTGGCATTTTATGCCAAGA+GGG - chr6.3:73531737-73531756 MS.gene37812:CDS 40.0%
! TTGCTGCTTGGTAATTAGGT+TGG - chr6.3:73532640-73532659 MS.gene37812:intron 40.0%
!! ATGCCTTTTGTTTACCTGGT+GGG - chr6.3:73532244-73532263 MS.gene37812:intron 40.0%
!! CTATTTGGAGTTGTTGGTTC+AGG - chr6.3:73531802-73531821 MS.gene37812:intron 40.0%
!! CTTGCGAGTCAACTTGTTTT+GGG + chr6.3:73532995-73533014 None:intergenic 40.0%
!! GATTCTAGTTTCGAATGCCA+TGG - chr6.3:73531888-73531907 MS.gene37812:intron 40.0%
!! GCATTTGGATGGACTGAATT+GGG - chr6.3:73532191-73532210 MS.gene37812:intron 40.0%
!! GTTGATGCCTTTTGTTTACC+TGG - chr6.3:73532240-73532259 MS.gene37812:intron 40.0%
!!! GCTAGTCTTTTGAGTTTGTG+CGG + chr6.3:73533030-73533049 None:intergenic 40.0%
AATGCCATGGAGAAGAAGCT+TGG - chr6.3:73531901-73531920 MS.gene37812:intron 45.0%
ACCCTCAAAGTGTGAGAGTT+GGG - chr6.3:73531983-73532002 MS.gene37812:intron 45.0%
AGACTGAGCGGAGTGATTTA+GGG - chr6.3:73532055-73532074 MS.gene37812:intron 45.0%
AGTTGAAGGGAATTGGCAGA+TGG - chr6.3:73532101-73532120 MS.gene37812:intron 45.0%
ATAGCGAGATCGGGAAATTC+TGG + chr6.3:73531602-73531621 None:intergenic 45.0%
ATCCCAACTCTCACACTTTG+AGG + chr6.3:73531988-73532007 None:intergenic 45.0%
CATGTCACAGGAACAATGCT+AGG + chr6.3:73533717-73533736 None:intergenic 45.0%
CATTGAACAAGGAGACTGAG+CGG - chr6.3:73532043-73532062 MS.gene37812:intron 45.0%
CCATCCAAATGCGACGTATA+AGG + chr6.3:73532183-73532202 None:intergenic 45.0%
CCTTATACGTCGCATTTGGA+TGG - chr6.3:73532180-73532199 MS.gene37812:intron 45.0%
CTTCCCACCAGGTAAACAAA+AGG + chr6.3:73532250-73532269 None:intergenic 45.0%
CTTCTATTAGTCGCATCAGC+AGG - chr6.3:73533948-73533967 MS.gene37812:CDS 45.0%
GATTGTGGAAAGGTGAAGGA+CGG - chr6.3:73533844-73533863 MS.gene37812:CDS 45.0%
GCAACCTTATACGTCGCATT+TGG - chr6.3:73532176-73532195 MS.gene37812:intron 45.0%
GCGGCCCTTTCTTTAAATCA+AGG - chr6.3:73533112-73533131 MS.gene37812:intron 45.0%
GGGTATGGTTCAGAGAATGA+AGG - chr6.3:73532075-73532094 MS.gene37812:intron 45.0%
GGTTCGAGGCAATTCGATAA+GGG + chr6.3:73533313-73533332 None:intergenic 45.0%
TCCCAACTCTCACACTTTGA+GGG + chr6.3:73531987-73532006 None:intergenic 45.0%
TGGTTCGAGGCAATTCGATA+AGG + chr6.3:73533314-73533333 None:intergenic 45.0%
TGTATTGCATGCTCATGCTG+AGG - chr6.3:73534100-73534119 MS.gene37812:CDS 45.0%
! AACCAATCTTTTGCCCCTCT+TGG + chr6.3:73531754-73531773 None:intergenic 45.0%
! ACGTGTGTTCGGTTTGTGTA+AGG + chr6.3:73531868-73531887 None:intergenic 45.0%
! AGCAACTTTTATCGGACGTG+AGG - chr6.3:73532320-73532339 MS.gene37812:intron 45.0%
! CAAATGGGTTCAGAAGGGTA+AGG - chr6.3:73532143-73532162 MS.gene37812:intron 45.0%
! GGTGAAGGACGGTAATTTGT+TGG - chr6.3:73533855-73533874 MS.gene37812:CDS 45.0%
! GTGGCATTTTATGCCAAGAG+GGG - chr6.3:73531738-73531757 MS.gene37812:CDS 45.0%
! GTGTGGCATTTTATGCCAAG+AGG - chr6.3:73531736-73531755 MS.gene37812:CDS 45.0%
!! CGCATTTGGATGGACTGAAT+TGG - chr6.3:73532190-73532209 MS.gene37812:intron 45.0%
!! GATCGGGAAATTCTGGAGTT+AGG + chr6.3:73531595-73531614 None:intergenic 45.0%
!! GATGCCTTTTGTTTACCTGG+TGG - chr6.3:73532243-73532262 MS.gene37812:intron 45.0%
!! GCTTGCGAGTCAACTTGTTT+TGG + chr6.3:73532996-73533015 None:intergenic 45.0%
AAACTGCTCAGCACTGTGTC+TGG + chr6.3:73532677-73532696 None:intergenic 50.0%
AGCAGGCTATGATCCTCATG+TGG - chr6.3:73533965-73533984 MS.gene37812:CDS 50.0%
AGGTTCTGTAACAATGCCGC+TGG + chr6.3:73533637-73533656 None:intergenic 50.0%
CACCCTCAAAGTGTGAGAGT+TGG - chr6.3:73531982-73532001 MS.gene37812:intron 50.0%
GAGACTGAGCGGAGTGATTT+AGG - chr6.3:73532054-73532073 MS.gene37812:intron 50.0%
GCTCGCTAAAGCTCAGGTAA+TGG - chr6.3:73534178-73534197 MS.gene37812:CDS 50.0%
GTCTCCAAGCTTCTTCTCCA+TGG + chr6.3:73531908-73531927 None:intergenic 50.0%
GTGATACGCCCAATCCTCAT+CGG - chr6.3:73533605-73533624 MS.gene37812:CDS 50.0%
TGATACGCCCAATCCTCATC+GGG - chr6.3:73533606-73533625 MS.gene37812:CDS 50.0%
TGATCCTCATGTGGCTCCTA+CGG - chr6.3:73533974-73533993 MS.gene37812:CDS 50.0%
TGCCAAGAGGGGCAAAAGAT+TGG - chr6.3:73531749-73531768 MS.gene37812:CDS 50.0%
TTTGGCGACCTTTCGGAGAA+AGG - chr6.3:73532764-73532783 MS.gene37812:intron 50.0%
! ACACTTTGAGGGTGTGTTGC+CGG + chr6.3:73531976-73531995 None:intergenic 50.0%
! CTGAGCTTTAGCGAGCGATT+CGG + chr6.3:73534174-73534193 None:intergenic 50.0%
! CTGTAACAATGCCGCTGGTT+TGG + chr6.3:73533632-73533651 None:intergenic 50.0%
! GAGCGGAGTGATTTAGGGTA+TGG - chr6.3:73532060-73532079 MS.gene37812:intron 50.0%
!!! GCGAGTCAACTTGTTTTGGG+AGG + chr6.3:73532992-73533011 None:intergenic 50.0%
!!! GTTTTGGGAGGCCAAAATCG+TGG + chr6.3:73532980-73532999 None:intergenic 50.0%
AAGACCGTAGGAGCCACATG+AGG + chr6.3:73533981-73534000 None:intergenic 55.0%
CGAATCGCTCGCTAAAGCTC+AGG - chr6.3:73534172-73534191 MS.gene37812:CDS 55.0%
GGTTTGGCCCGATGAGGATT+GGG + chr6.3:73533616-73533635 None:intergenic 55.0%
! TGGTTTGGCCCGATGAGGAT+TGG + chr6.3:73533617-73533636 None:intergenic 55.0%
TCCTCATCGGGCCAAACCAG+CGG - chr6.3:73533618-73533637 MS.gene37812:CDS 60.0%
!! GCCGCTGGTTTGGCCCGATG+AGG + chr6.3:73533622-73533641 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 73531490 73534238 73531490 ID=MS.gene37812
chr6.3 mRNA 73531490 73534238 73531490 ID=MS.gene37812.t1;Parent=MS.gene37812
chr6.3 exon 73533387 73534238 73533387 ID=MS.gene37812.t1.exon1;Parent=MS.gene37812.t1
chr6.3 CDS 73533387 73534238 73533387 ID=cds.MS.gene37812.t1;Parent=MS.gene37812.t1
chr6.3 exon 73532943 73533070 73532943 ID=MS.gene37812.t1.exon2;Parent=MS.gene37812.t1
chr6.3 CDS 73532943 73533070 73532943 ID=cds.MS.gene37812.t1;Parent=MS.gene37812.t1
chr6.3 exon 73531490 73531808 73531490 ID=MS.gene37812.t1.exon3;Parent=MS.gene37812.t1
chr6.3 CDS 73531490 73531808 73531490 ID=cds.MS.gene37812.t1;Parent=MS.gene37812.t1
Gene Sequence

>MS.gene37812

ATGGCTTGGTTTAGAAGAACAGAAAAGCTTATTGCTCAAGCTTTTCATGCATTGCCTTCAACTTCAACTTCAAGGCTTCCATCTCATAACTCTATTTTTCAGCCTAACTCCAGAATTTCCCGATCTCGCTATTTTAATTTCGCTACCAAAGGTGGTCCTTATAATAGGTTTTCTCAGAGAATAGGAACAAGTGGTTTTGAAATTAATAACAATTTTAGGAGATTTTACTCTGTGTATCCTAAAAATGTGTGGCATTTTATGCCAAGAGGGGCAAAAGATTGGTTTCAAGATCCTAAAAATGTTTCCCTTGTTCTATTTGGAGTTGTTGGTTCAGGTGTTTTAATTACCGTGTGTTTACAAAATATGGAAACTATTCCTTACACAAACCGAACACACGTGATTCTAGTTTCGAATGCCATGGAGAAGAAGCTTGGAGACTGCGTGTTTGAAAAGATGAAGGCAGACTTTAAAAATGAGTTGTTGCCGGCAACACACCCTCAAAGTGTGAGAGTTGGGATGATAGCAAACAATATTCTCGATGCGATGAAGAAAACATTGAACAAGGAGACTGAGCGGAGTGATTTAGGGTATGGTTCAGAGAATGAAGGAAAAGTTGAAGGGAATTGGCAGATGGAGAATGAGAAACTTGATGACAAATGGGTTCAGAAGGGTAAGGAACAAGATTCGCAACCTTATACGTCGCATTTGGATGGACTGAATTGGGAGATTTTGGTGGTGAATAAGCCTTTTGTTGATGCCTTTTGTTTACCTGGTGGGAAGATAGTTGTATTCACTGGTTTGCTTGAACATTTTAGTAGTGATGCAGAGATAGCAACTTTTATCGGACGTGAGGTATTCATTTCATTCGCTCTTTATGTCTGTTTGGATTAACTTATTTGAATTTATCTACTTGCTAAGCGCTTCTGAGTCTATTTGAGAGATATTATGGAAACAATTTATGACATGATATATGTTTACAGCTTAAATGAAAACAAATTGACTTTATTTTATCTTTTGTTATAGAAACAGCTTATACTTAAGCACTTGTAGAACAAAAGCTTATGCGAAACGTGCTTAATTGAGCTATTTATCCAAACAAGGTCATTTTTTTTGCAGTTTAATATGTTTAAGAGACATAAAATCTAACACTTTGCTGCTTGGTAATTAGGTTGGACATGTTGTAGCCAGACACAGTGCTGAGCAGTTTACACATAATCAGTGGTTTTCAGAACTCAGACATAGTAGTGATAATAACAGACTGTCATCACTTTTCTTTTGGCGACCTTTCGGAGAAAGGTAATGATTCCTCCATTGTTTATCTTTATAACAATTGAATTTTTGTGATGTTCTTTAATAATTCATTGATATATAAGTAATGTTTGTTAGACAGGTTAGAATAGGAGTGCACATTAAATATTAGCTAGTAAGAGAAGTGAAAAACATGAAATTAGGTCAAAGACTAATAAATTTTGGCTCAATTAGTGTTACCACGATTTTGGCCTCCCAAAACAAGTTGACTCGCAAGCAGAATTGTTTTCCGCACAAACTCAAAAGACTAGCATTTTTTTTTTTTTGCGAGTTGACAAGTTCGTCAAAAAATAAAAAAAAAGTAAACTTAAAACGCGGCCCTTTCTTTAAATCAAGGGTTGCGAATCATGTCCTTCGCATAAATGAATGATTATTAACTGTTATATGAATGATTAAATGTTCTCGGAGTTATTAACTTATTATACCTAGTAGTACCTACTCTCTAGAATAATTATCGTTCGGTTGAGGAAGCAAACCGAACTGTTACCACAGTTCAGAAAAATTGAACTGAACCCTTATCGAATTGCCTCGAACCAACTTTTATAGAGATTTGGTGCACCATTTCTGTAGTTTCTAAACCAAACTGGGATTTTTTTAACCAAATTATACTAAGGATAACTAGGACAATTGAAATTAAATAAAAGACCGAACCAAATCTAGTATTAGAAATTAGGACTATTTCAAAAAGACAAAAACCAAATTGCTGAACTGAACTGCAACTATATTTCATAAATTGAACTAAACCGAACTGGTTTCGTATAGTTCAAGAACTGTTAGTCACAGTTTGATTCTTTTTCAATGTTGTTCGTGATACGCCCAATCCTCATCGGGCCAAACCAGCGGCATTGTTACAGAACCTGCAGCATTGTTATGAACTAGTGAAACGTATGAGCTGGTAGTGATAGCTAGAATATTCCCTAGCATTGTTCCTGTGACATGATCTAAAATTATAGGAATAGGCAACAAATCCACTTGTGTAATTAAGCCCACATTTCTTGGTTGAAATAGAAAATTGTATAATTTTAGGCGTTTTAGGATGCAATATTGATTGTGGAAAGGTGAAGGACGGTAATTTGTTGGTTAAAAATTTGTGACATAACAAAATTTATTGACGACATTCAGGATGGAAAAAGAAGCTGATTACATTGGGCTTCTATTAGTCGCATCAGCAGGCTATGATCCTCATGTGGCTCCTACGGTCTTAGAGAAATTCATTGATCGTGATTCTATGCTTACAGATTATCGCTCTACTCAACATCGACCTCATATATCTAAAGCTGAAGCCGAGGCAAAATATAGAGCTGTATTGCATGCTCATGCTGAGGAAAAAAAAAGAGCTGAATTGCTCTTTCAAGCTCAGGTAAGAAAAAGAGCCGAATCGCTCGCTAAAGCTCAGGTAATGGATGAAGCTCTTACTATATATGAGAATGTAAGAGCTTGA
Protein sequence

>MS.gene37812.t1

MAWFRRTEKLIAQAFHALPSTSTSRLPSHNSIFQPNSRISRSRYFNFATKGGPYNRFSQRIGTSGFEINNNFRRFYSVYPKNVWHFMPRGAKDWFQDPKNVSLVLFGVVGSGVLITVCLQNMETIPYTNRTHVILVSNAMEKKLGDCVFEKMKADFKNELLPATHPQSVRVGMIANNILDAMKKTLNKETERSDLGYGSENEGKVEGNWQMENEKLDDKWVQKGKEQDSQPYTSHLDGLNWEILVVNKPFVDAFCLPGGKIVVFTGLLEHFSSDAEIATFIGREVGHVVARHSAEQFTHNQWFSELRHSSDNNRLSSLFFWRPFGERMEKEADYIGLLLVASAGYDPHVAPTVLEKFIDRDSMLTDYRSTQHRPHISKAEAEAKYRAVLHAHAEEKKRAELLFQAQVRKRAESLAKAQVMDEALTIYENVRA