AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05277

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05277.t1 MTR_6g079410 70.435 345 67 4 1 310 119 463 3.01e-164 465
MS.gene05277.t1 MTR_3g006860 56.305 341 85 6 1 310 214 521 1.15e-113 339
MS.gene05277.t1 MTR_6g463380 55.425 341 88 6 1 310 218 525 3.23e-112 335
MS.gene05277.t1 MTR_6g463380 58.650 237 69 3 1 208 218 454 1.92e-85 264
MS.gene05277.t1 MTR_6g463380 58.723 235 68 3 1 206 218 452 1.86e-84 261
MS.gene05277.t1 MTR_6g061650 43.210 324 115 10 1 311 46 313 1.36e-63 204
MS.gene05277.t1 MTR_1g014390 34.385 317 113 6 1 311 62 289 1.86e-46 159
MS.gene05277.t1 MTR_6g006850 32.087 321 156 9 3 311 32 302 5.75e-41 145
MS.gene05277.t1 MTR_6g006710 30.435 299 131 7 15 310 63 287 2.01e-32 122
MS.gene05277.t1 MTR_1g029560 36.098 205 94 5 110 311 27 197 3.53e-29 110
MS.gene05277.t1 MTR_1g012010 33.645 214 96 3 2 208 66 240 1.46e-28 110
MS.gene05277.t1 MTR_6g080120 85.106 47 7 0 207 253 1 47 6.20e-22 87.8
MS.gene05277.t1 MTR_7g025575 41.748 103 32 3 207 308 13 88 1.68e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05277.t1 AT5G51740 47.076 342 115 8 2 311 126 433 2.54e-93 284
MS.gene05277.t1 AT5G51740 47.076 342 115 8 2 311 169 476 6.34e-93 284
MS.gene05277.t1 AT5G51740 48.085 235 92 4 2 206 169 403 4.50e-69 220

Find 54 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGACTGATCGGAGTGATTT+AGG 0.166851 6.2:-78668704 MS.gene05277:CDS
TGGACTGAATTGGGAGATTT+TGG 0.167567 6.2:-78668558 MS.gene05277:CDS
GCAACCTTATACGTCGCATT+TGG 0.332750 6.2:-78668582 MS.gene05277:CDS
CCATCCAAATGCGACGTATA+AGG 0.337352 6.2:+78668578 None:intergenic
CGCATTTGGATGGACTGAAT+TGG 0.338297 6.2:-78668568 MS.gene05277:CDS
GATGAAGAAAACATTGAATA+AGG 0.349127 6.2:-78668726 MS.gene05277:CDS
ATGAGAAACTTGATGACAAA+TGG 0.362150 6.2:-78668631 MS.gene05277:CDS
ACACTTTGAGGGTGTGTTGC+CGG 0.386983 6.2:+78668785 None:intergenic
AAGGAAAAGTTGAAGGGAAT+TGG 0.390236 6.2:-78668664 MS.gene05277:CDS
AGACTGATCGGAGTGATTTA+GGG 0.392381 6.2:-78668703 MS.gene05277:CDS
CTGAGCTTTAGCGAGCGATT+CGG 0.424071 6.2:+78666598 None:intergenic
AAACTAGAATCACGTGTGTT+CGG 0.433419 6.2:+78668882 None:intergenic
GCATTTGGATGGACTGAATT+GGG 0.433979 6.2:-78668567 MS.gene05277:CDS
ACCCTCAAAGTGTGAGAGTT+GGG 0.436760 6.2:-78668775 MS.gene05277:CDS
ACGTGTGTTCGGTTTGTGTA+AGG 0.437622 6.2:+78668893 None:intergenic
TGAATTGCTCTTTCAAGCTC+AGG 0.442207 6.2:-78666633 MS.gene05277:CDS
ATCCCAACTCTCACACTTTG+AGG 0.443157 6.2:+78668773 None:intergenic
GCTAAAATGTTCAAGCAAAC+CGG 0.446177 6.2:+78668475 None:intergenic
TTTGGCGACCTTTCGGAGAA+AGG 0.449893 6.2:-78667994 MS.gene05277:intron
AAACTGCTCAGCACTGTGTC+TGG 0.465687 6.2:+78668084 None:intergenic
GCTCGCTAAAGCTCAGGTAA+TGG 0.469980 6.2:-78666591 MS.gene05277:CDS
TGATGACAAATGGGTTAAGA+AGG 0.473472 6.2:-78668621 MS.gene05277:CDS
GATCGGAGTGATTTAGGGTA+TGG 0.474536 6.2:-78668698 MS.gene05277:CDS
GAAAATGAAGGAAAAGTTGA+AGG 0.478389 6.2:-78668671 MS.gene05277:CDS
CCTTATACGTCGCATTTGGA+TGG 0.481840 6.2:-78668578 MS.gene05277:CDS
AAACAAAAGGCATCAACAAA+AGG 0.483071 6.2:+78668524 None:intergenic
GCTTCAGCTTTAGATATATG+AGG 0.496330 6.2:+78666710 None:intergenic
GTCTCCAAGCTTCTTCTCCA+TGG 0.500499 6.2:+78668853 None:intergenic
TCCCAACTCTCACACTTTGA+GGG 0.505571 6.2:+78668774 None:intergenic
TGAGAAACTTGATGACAAAT+GGG 0.508994 6.2:-78668630 MS.gene05277:CDS
CGAATCGCTCGCTAAAGCTC+AGG 0.514358 6.2:-78666597 MS.gene05277:CDS
TGATCCTCATGTGGCTCCTA+CGG 0.519441 6.2:-78666795 MS.gene05277:CDS
CTTCTATTAGTCGCATCAGC+AGG 0.528066 6.2:-78666821 MS.gene05277:CDS
AATGCCATGGAGAAGAAGCT+TGG 0.530715 6.2:-78668857 MS.gene05277:CDS
GGGTATGGTTCAGAAAATGA+AGG 0.531448 6.2:-78668683 MS.gene05277:CDS
TGAAGGGAATTGGCAGATGG+AGG 0.541512 6.2:-78668654 MS.gene05277:CDS
CTTCCCACCAGGTAAACAAA+AGG 0.543528 6.2:+78668511 None:intergenic
GGGAAGATAGTTGTATTCAC+CGG 0.553263 6.2:-78668494 MS.gene05277:CDS
CTGCGTGTTTGAAAAGATGA+AGG 0.567504 6.2:-78668831 MS.gene05277:CDS
CACCCTCAAAGTGTGAGAGT+TGG 0.577769 6.2:-78668776 MS.gene05277:CDS
GATTCTAGTTTCGAATGCCA+TGG 0.579969 6.2:-78668870 MS.gene05277:CDS
AAAATGAAGGAAAAGTTGAA+GGG 0.590138 6.2:-78668670 MS.gene05277:CDS
AGTTGAAGGGAATTGGCAGA+TGG 0.592584 6.2:-78668657 MS.gene05277:CDS
AATCATTACCTTTCTCCGAA+AGG 0.593435 6.2:+78667986 None:intergenic
ATGAATTTCTCTAAGACCGT+AGG 0.593712 6.2:+78666779 None:intergenic
GATGACAAATGGGTTAAGAA+GGG 0.606043 6.2:-78668620 MS.gene05277:CDS
AGCAGTTTACACATAATCAG+TGG 0.621012 6.2:-78668069 MS.gene05277:CDS
TATATCTAAAGCTGAAGCCG+AGG 0.624435 6.2:-78666705 MS.gene05277:CDS
CATTGAATAAGGAGACTGAT+CGG 0.625254 6.2:-78668715 MS.gene05277:CDS
CAAATGGGTTAAGAAGGGTA+AGG 0.625766 6.2:-78668615 MS.gene05277:CDS
AATACAACTATCTTCCCACC+AGG 0.658440 6.2:+78668500 None:intergenic
AGCAGGCTATGATCCTCATG+TGG 0.663662 6.2:-78666804 MS.gene05277:CDS
TGTATTGCATGCTCATGCTG+AGG 0.685341 6.2:-78666669 MS.gene05277:CDS
AAGACCGTAGGAGCCACATG+AGG 0.724893 6.2:+78666791 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGTTATAAAGATAAACAA+TGG + chr6.2:78667501-78667520 None:intergenic 15.0%
!!! TTTTTAACCAAATTATACTA+AGG - chr6.2:78668078-78668097 MS.gene05277:CDS 15.0%
!! AAATTATACTAAGGATAACT+AGG - chr6.2:78668087-78668106 MS.gene05277:CDS 20.0%
!! CAAATCAAGTATTAGAAATT+AGG - chr6.2:78668138-78668157 MS.gene05277:intron 20.0%
!! CTAATTTCTAATACTTGATT+TGG + chr6.2:78668140-78668159 None:intergenic 20.0%
!! GATGTTCTTTAATAATTCAT+TGG - chr6.2:78667532-78667551 MS.gene05277:intron 20.0%
!! TAGTTATCCTTAGTATAATT+TGG + chr6.2:78668088-78668107 None:intergenic 20.0%
!! TATGAATGATTAAATGTTCT+CGG - chr6.2:78667875-78667894 MS.gene05277:intron 20.0%
!!! TGATCTAAAATTATAGGAAT+AGG - chr6.2:78668424-78668443 MS.gene05277:intron 20.0%
!!! TGTATAATATTAAGCGTTTT+AGG - chr6.2:78668500-78668519 MS.gene05277:CDS 20.0%
! AAAAAGAAGCTGATTACATT+GGG - chr6.2:78668614-78668633 MS.gene05277:CDS 25.0%
! AAAATGAAGGAAAAGTTGAA+GGG - chr6.2:78666788-78666807 MS.gene05277:CDS 25.0%
! AAAATTTATTGACGACATTC+AGG - chr6.2:78668587-78668606 MS.gene05277:CDS 25.0%
! AGTCTATTTGAGAGATATTA+TGG - chr6.2:78667116-78667135 MS.gene05277:intron 25.0%
! ATAATCATTCATTTATGCGA+AGG + chr6.2:78667846-78667865 None:intergenic 25.0%
! GAAGTGAAAAACATGAAATT+AGG - chr6.2:78667601-78667620 MS.gene05277:intron 25.0%
! GATGAAGAAAACATTGAATA+AGG - chr6.2:78666732-78666751 MS.gene05277:CDS 25.0%
! GTTATAAAGATAAACAATGG+AGG + chr6.2:78667498-78667517 None:intergenic 25.0%
! TTAATAATTCATTGGTAGAC+AGG - chr6.2:78667540-78667559 MS.gene05277:intron 25.0%
!! AACCAACTTTTATAGAGATT+TGG - chr6.2:78668018-78668037 MS.gene05277:CDS 25.0%
!! ATAATTTTAGATCATGTCAC+AGG + chr6.2:78668418-78668437 None:intergenic 25.0%
!! GGTCAAAGACTAATAAATTT+TGG - chr6.2:78667622-78667641 MS.gene05277:intron 25.0%
!! GTGACATGATCTAAAATTAT+AGG - chr6.2:78668418-78668437 MS.gene05277:intron 25.0%
!!! CCTCTAGAATAATTATCATT+CGG - chr6.2:78667926-78667945 MS.gene05277:intron 25.0%
!!! TTCTAATACTTGATTTGGTT+CGG + chr6.2:78668135-78668154 None:intergenic 25.0%
CAACCCTTGATTTAAAGAAA+GGG + chr6.2:78667813-78667832 None:intergenic 30.0%
CACCAAATCTCTATAAAAGT+TGG + chr6.2:78668023-78668042 None:intergenic 30.0%
CCGAATGATAATTATTCTAG+AGG + chr6.2:78667929-78667948 None:intergenic 30.0%
CTATTTCAACCAAGAAATGT+GGG + chr6.2:78668475-78668494 None:intergenic 30.0%
GAAAAAGAAGCTGATTACAT+TGG - chr6.2:78668613-78668632 MS.gene05277:CDS 30.0%
GAAAATGAAGGAAAAGTTGA+AGG - chr6.2:78666787-78666806 MS.gene05277:CDS 30.0%
GAATAATTATCATTCGGTTC+AGG - chr6.2:78667932-78667951 MS.gene05277:intron 30.0%
GCAAAAAAAATGACCTTGTT+TGG + chr6.2:78667284-78667303 None:intergenic 30.0%
TAAATTGAACTAAACCGAAC+TGG - chr6.2:78668216-78668235 MS.gene05277:intron 30.0%
TCTATTTCAACCAAGAAATG+TGG + chr6.2:78668476-78668495 None:intergenic 30.0%
TGGTTAAAAAAATCCCAGTT+TGG + chr6.2:78668068-78668087 None:intergenic 30.0%
TGGTTTAGAAACTACAGAAA+TGG + chr6.2:78668048-78668067 None:intergenic 30.0%
TGTAGTTTCTAAACCAAACT+GGG - chr6.2:78668052-78668071 MS.gene05277:CDS 30.0%
TTGAGCTATTTATCCAAACA+AGG - chr6.2:78667268-78667287 MS.gene05277:intron 30.0%
! AGTTCAATTTTTCTGAACTG+TGG + chr6.2:78667976-78667995 None:intergenic 30.0%
! ATGAGAAACTTGATGACAAA+TGG - chr6.2:78666827-78666846 MS.gene05277:CDS 30.0%
! ATGCAATATTGATTGTGGAA+AGG - chr6.2:78668523-78668542 MS.gene05277:CDS 30.0%
! CTTTAAAAATGAGTTGTTGC+CGG - chr6.2:78666654-78666673 MS.gene05277:CDS 30.0%
! TATTGATTGTGGAAAGGTTA+AGG - chr6.2:78668529-78668548 MS.gene05277:CDS 30.0%
! TGAGAAACTTGATGACAAAT+GGG - chr6.2:78666828-78666847 MS.gene05277:CDS 30.0%
! TTAGGATGCAATATTGATTG+TGG - chr6.2:78668518-78668537 MS.gene05277:CDS 30.0%
!! AAACAAAAGGCATCAACAAA+AGG + chr6.2:78666937-78666956 None:intergenic 30.0%
!! ATTATTCTAGAGGTACTACT+AGG + chr6.2:78667919-78667938 None:intergenic 30.0%
AAACTAGAATCACGTGTGTT+CGG + chr6.2:78666579-78666598 None:intergenic 35.0%
AAATCTAACACTTTGCTGCT+TGG - chr6.2:78667328-78667347 MS.gene05277:intron 35.0%
AAGGAAAAGTTGAAGGGAAT+TGG - chr6.2:78666794-78666813 MS.gene05277:CDS 35.0%
AATCATTACCTTTCTCCGAA+AGG + chr6.2:78667475-78667494 None:intergenic 35.0%
AGCAGTTTACACATAATCAG+TGG - chr6.2:78667389-78667408 MS.gene05277:intron 35.0%
ATTCGCTCTTTATGTCTGTT+TGG - chr6.2:78667053-78667072 MS.gene05277:intron 35.0%
ATTGGTAGACAGGTTAGAAT+AGG - chr6.2:78667550-78667569 MS.gene05277:intron 35.0%
CAGCCCTTTCTTTAAATCAA+GGG - chr6.2:78667807-78667826 MS.gene05277:intron 35.0%
CATTGAATAAGGAGACTGAT+CGG - chr6.2:78666743-78666762 MS.gene05277:CDS 35.0%
CTGTAGTTTCTAAACCAAAC+TGG - chr6.2:78668051-78668070 MS.gene05277:CDS 35.0%
GCAACCCTTGATTTAAAGAA+AGG + chr6.2:78667814-78667833 None:intergenic 35.0%
GCTAAAATGTTCAAGCAAAC+CGG + chr6.2:78666986-78667005 None:intergenic 35.0%
GTAATTAAGCCCACATTTCT+TGG - chr6.2:78668463-78668482 MS.gene05277:CDS 35.0%
TGAACTATACGAAACCAGTT+CGG + chr6.2:78668233-78668252 None:intergenic 35.0%
TTTATTGACGACATTCAGGA+TGG - chr6.2:78668591-78668610 MS.gene05277:CDS 35.0%
! GCAGAGATAGCAACTTTTAT+CGG - chr6.2:78667012-78667031 MS.gene05277:intron 35.0%
! TACAGCTCTATATTTTGCCT+CGG + chr6.2:78668773-78668792 None:intergenic 35.0%
!! ATGAATTTCTCTAAGACCGT+AGG + chr6.2:78668682-78668701 None:intergenic 35.0%
!! GACTGTCATCACTTTTCTTT+TGG - chr6.2:78667446-78667465 MS.gene05277:intron 35.0%
!! GATGACAAATGGGTTAAGAA+GGG - chr6.2:78666838-78666857 MS.gene05277:CDS 35.0%
!! GCTTCAGCTTTAGATATATG+AGG + chr6.2:78668751-78668770 None:intergenic 35.0%
!! TCTCTATAAAAGTTGGTTCG+AGG + chr6.2:78668016-78668035 None:intergenic 35.0%
!! TGATGACAAATGGGTTAAGA+AGG - chr6.2:78666837-78666856 MS.gene05277:CDS 35.0%
AACTAGTGAAACGTATGAGC+TGG - chr6.2:78668360-78668379 MS.gene05277:intron 40.0%
AATACAACTATCTTCCCACC+AGG + chr6.2:78666961-78666980 None:intergenic 40.0%
AGACTGATCGGAGTGATTTA+GGG - chr6.2:78666755-78666774 MS.gene05277:CDS 40.0%
ATGTCACAGGAACAATGCTA+GGG + chr6.2:78668405-78668424 None:intergenic 40.0%
CTAGTTCATAACAATGCTGC+AGG + chr6.2:78668346-78668365 None:intergenic 40.0%
CTGCGTGTTTGAAAAGATGA+AGG - chr6.2:78666627-78666646 MS.gene05277:CDS 40.0%
GATTGTGGAAAGGTTAAGGA+CGG - chr6.2:78668533-78668552 MS.gene05277:CDS 40.0%
GCAGCCCTTTCTTTAAATCA+AGG - chr6.2:78667806-78667825 MS.gene05277:intron 40.0%
GGAGGCCAAAATTGTAAACT+CGG + chr6.2:78667667-78667686 None:intergenic 40.0%
GGGAAGATAGTTGTATTCAC+CGG - chr6.2:78666964-78666983 MS.gene05277:intron 40.0%
GGGTATGGTTCAGAAAATGA+AGG - chr6.2:78666775-78666794 MS.gene05277:CDS 40.0%
TCTGAACTGTGGTAACAGTT+CGG + chr6.2:78667965-78667984 None:intergenic 40.0%
TGAATTGCTCTTTCAAGCTC+AGG - chr6.2:78668825-78668844 MS.gene05277:CDS 40.0%
TGCAGTTCAGTTCAGCAATT+TGG + chr6.2:78668185-78668204 None:intergenic 40.0%
TGTGGGCTTAATTACACAAG+TGG + chr6.2:78668458-78668477 None:intergenic 40.0%
TTGGCTCAATTAGTGTTACC+AGG - chr6.2:78667641-78667660 MS.gene05277:intron 40.0%
! ACTGAATTGGGAGATTTTGG+TGG - chr6.2:78666903-78666922 MS.gene05277:intron 40.0%
! CACTTTGCTGCTTGGTAATT+AGG - chr6.2:78667336-78667355 MS.gene05277:intron 40.0%
! CTTTTCTTTTGGCGACCTTT+CGG - chr6.2:78667457-78667476 MS.gene05277:intron 40.0%
! GGTTAAGGACGGTAATTTGT+TGG - chr6.2:78668544-78668563 MS.gene05277:CDS 40.0%
! TATATCTAAAGCTGAAGCCG+AGG - chr6.2:78668753-78668772 MS.gene05277:CDS 40.0%
! TGGACTGAATTGGGAGATTT+TGG - chr6.2:78666900-78666919 MS.gene05277:intron 40.0%
! TTGCTGCTTGGTAATTAGGT+TGG - chr6.2:78667340-78667359 MS.gene05277:intron 40.0%
!! ATGCCTTTTGTTTACCTGGT+GGG - chr6.2:78666944-78666963 MS.gene05277:intron 40.0%
!! CAAATGGGTTAAGAAGGGTA+AGG - chr6.2:78666843-78666862 MS.gene05277:CDS 40.0%
!! CTTGCGAGTCAACTTGTTTT+GGG + chr6.2:78667688-78667707 None:intergenic 40.0%
!! GATTCTAGTTTCGAATGCCA+TGG - chr6.2:78666588-78666607 MS.gene05277:CDS 40.0%
!! GCATTTGGATGGACTGAATT+GGG - chr6.2:78666891-78666910 MS.gene05277:intron 40.0%
!! GTTGATGCCTTTTGTTTACC+TGG - chr6.2:78666940-78666959 MS.gene05277:intron 40.0%
AATGCCATGGAGAAGAAGCT+TGG - chr6.2:78666601-78666620 MS.gene05277:CDS 45.0%
ACCCTCAAAGTGTGAGAGTT+GGG - chr6.2:78666683-78666702 MS.gene05277:CDS 45.0%
AGTTGAAGGGAATTGGCAGA+TGG - chr6.2:78666801-78666820 MS.gene05277:CDS 45.0%
ATCCCAACTCTCACACTTTG+AGG + chr6.2:78666688-78666707 None:intergenic 45.0%
CATGTCACAGGAACAATGCT+AGG + chr6.2:78668406-78668425 None:intergenic 45.0%
CCAAAATTGTAAACTCGGCC+TGG + chr6.2:78667662-78667681 None:intergenic 45.0%
CCATCCAAATGCGACGTATA+AGG + chr6.2:78666883-78666902 None:intergenic 45.0%
CCTTATACGTCGCATTTGGA+TGG - chr6.2:78666880-78666899 MS.gene05277:intron 45.0%
CTTCCCACCAGGTAAACAAA+AGG + chr6.2:78666950-78666969 None:intergenic 45.0%
CTTCTATTAGTCGCATCAGC+AGG - chr6.2:78668637-78668656 MS.gene05277:CDS 45.0%
GAGACTGATCGGAGTGATTT+AGG - chr6.2:78666754-78666773 MS.gene05277:CDS 45.0%
GCAACCTTATACGTCGCATT+TGG - chr6.2:78666876-78666895 MS.gene05277:intron 45.0%
GGTTCGAGGCAATTCGATAA+GGG + chr6.2:78668002-78668021 None:intergenic 45.0%
TCCCAACTCTCACACTTTGA+GGG + chr6.2:78666687-78666706 None:intergenic 45.0%
TGGTTCGAGGCAATTCGATA+AGG + chr6.2:78668003-78668022 None:intergenic 45.0%
TGTATTGCATGCTCATGCTG+AGG - chr6.2:78668789-78668808 MS.gene05277:CDS 45.0%
! ACGTGTGTTCGGTTTGTGTA+AGG + chr6.2:78666568-78666587 None:intergenic 45.0%
! AGCAACTTTTATCGGACGTG+AGG - chr6.2:78667020-78667039 MS.gene05277:intron 45.0%
! CCAGGCCGAGTTTACAATTT+TGG - chr6.2:78667659-78667678 MS.gene05277:intron 45.0%
! GATCGGAGTGATTTAGGGTA+TGG - chr6.2:78666760-78666779 MS.gene05277:CDS 45.0%
!! CGCATTTGGATGGACTGAAT+TGG - chr6.2:78666890-78666909 MS.gene05277:intron 45.0%
!! GATGCCTTTTGTTTACCTGG+TGG - chr6.2:78666943-78666962 MS.gene05277:intron 45.0%
!! GCTTGCGAGTCAACTTGTTT+TGG + chr6.2:78667689-78667708 None:intergenic 45.0%
!!! GCGAGTCTTTTGAGTTTGTG+CGG + chr6.2:78667723-78667742 None:intergenic 45.0%
AAACTGCTCAGCACTGTGTC+TGG + chr6.2:78667377-78667396 None:intergenic 50.0%
AGCAGGCTATGATCCTCATG+TGG - chr6.2:78668654-78668673 MS.gene05277:CDS 50.0%
AGGTTCTGTAACAATGCCGC+TGG + chr6.2:78668326-78668345 None:intergenic 50.0%
CACCCTCAAAGTGTGAGAGT+TGG - chr6.2:78666682-78666701 MS.gene05277:CDS 50.0%
GCTCGCTAAAGCTCAGGTAA+TGG - chr6.2:78668867-78668886 MS.gene05277:CDS 50.0%
GTCTCCAAGCTTCTTCTCCA+TGG + chr6.2:78666608-78666627 None:intergenic 50.0%
GTGATACGCCCAATCCTCAT+CGG - chr6.2:78668294-78668313 MS.gene05277:intron 50.0%
TGAAGGGAATTGGCAGATGG+AGG - chr6.2:78666804-78666823 MS.gene05277:CDS 50.0%
TGATACGCCCAATCCTCATC+GGG - chr6.2:78668295-78668314 MS.gene05277:intron 50.0%
TGATCCTCATGTGGCTCCTA+CGG - chr6.2:78668663-78668682 MS.gene05277:CDS 50.0%
TTTGGCGACCTTTCGGAGAA+AGG - chr6.2:78667464-78667483 MS.gene05277:intron 50.0%
! ACACTTTGAGGGTGTGTTGC+CGG + chr6.2:78666676-78666695 None:intergenic 50.0%
! CTGAGCTTTAGCGAGCGATT+CGG + chr6.2:78668863-78668882 None:intergenic 50.0%
! CTGTAACAATGCCGCTGGTT+TGG + chr6.2:78668321-78668340 None:intergenic 50.0%
!!! GCGAGTCAACTTGTTTTGGG+AGG + chr6.2:78667685-78667704 None:intergenic 50.0%
AAGACCGTAGGAGCCACATG+AGG + chr6.2:78668670-78668689 None:intergenic 55.0%
CGAATCGCTCGCTAAAGCTC+AGG - chr6.2:78668861-78668880 MS.gene05277:CDS 55.0%
GGTTTGGCCCGATGAGGATT+GGG + chr6.2:78668305-78668324 None:intergenic 55.0%
! TGGTTTGGCCCGATGAGGAT+TGG + chr6.2:78668306-78668325 None:intergenic 55.0%
TCCTCATCGGGCCAAACCAG+CGG - chr6.2:78668307-78668326 MS.gene05277:intron 60.0%
!! GCCGCTGGTTTGGCCCGATG+AGG + chr6.2:78668311-78668330 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 78666553 78668927 78666553 ID=MS.gene05277
chr6.2 mRNA 78666553 78668927 78666553 ID=MS.gene05277.t1;Parent=MS.gene05277
chr6.2 exon 78668439 78668927 78668439 ID=MS.gene05277.t1.exon1;Parent=MS.gene05277.t1
chr6.2 CDS 78668439 78668927 78668439 ID=cds.MS.gene05277.t1;Parent=MS.gene05277.t1
chr6.2 exon 78667995 78668122 78667995 ID=MS.gene05277.t1.exon2;Parent=MS.gene05277.t1
chr6.2 CDS 78667995 78668122 78667995 ID=cds.MS.gene05277.t1;Parent=MS.gene05277.t1
chr6.2 exon 78666553 78666871 78666553 ID=MS.gene05277.t1.exon3;Parent=MS.gene05277.t1
chr6.2 CDS 78666553 78666871 78666553 ID=cds.MS.gene05277.t1;Parent=MS.gene05277.t1
Gene Sequence

>MS.gene05277

ATGGAAACTATTCCTTACACAAACCGAACACACGTGATTCTAGTTTCGAATGCCATGGAGAAGAAGCTTGGAGACTGCGTGTTTGAAAAGATGAAGGCAGACTTTAAAAATGAGTTGTTGCCGGCAACACACCCTCAAAGTGTGAGAGTTGGGATGATAGCGAACAATATTCTCGATGCGATGAAGAAAACATTGAATAAGGAGACTGATCGGAGTGATTTAGGGTATGGTTCAGAAAATGAAGGAAAAGTTGAAGGGAATTGGCAGATGGAGGATGAGAAACTTGATGACAAATGGGTTAAGAAGGGTAAGGAACAAGATTCGCAACCTTATACGTCGCATTTGGATGGACTGAATTGGGAGATTTTGGTGGTGAATAAGCCTTTTGTTGATGCCTTTTGTTTACCTGGTGGGAAGATAGTTGTATTCACCGGTTTGCTTGAACATTTTAGCAGTGATGCAGAGATAGCAACTTTTATCGGACGTGAGGTATTCATTTCATTCGCTCTTTATGTCTGTTTGGATTAACTTATTTGAATTTATATACTTGCTAAGCGCTTCTGAGTCTATTTGAGAGATATTATGGAAACAATTTATGACATGATATATGTTTACAGCTTAAATGAAAACAAATTGACTTTATTTTATCTTTTGTTATAGAAACAGCTTATACTTAAGCACTTGTATAACAAAAGCTTATGCGAAACGTGCTTAATTGAGCTATTTATCCAAACAAGGTCATTTTTTTTGCAGTTTAATATGTTTAAGAGACATAAAATCTAACACTTTGCTGCTTGGTAATTAGGTTGGACATGTTGTAGCCAGACACAGTGCTGAGCAGTTTACACATAATCAGTGGTTTTCAGAACTCAGACATAGTAGTGATAATAACAGACTGTCATCACTTTTCTTTTGGCGACCTTTCGGAGAAAGGTAATGATTCCTCCATTGTTTATCTTTATAACAATTGAATTTTTGTGATGTTCTTTAATAATTCATTGGTAGACAGGTTAGAATAGGAGTGCGCATTAAATATTAGATAGTAAGAGAAGTGAAAAACATGAAATTAGGTCAAAGACTAATAAATTTTGGCTCAATTAGTGTTACCAGGCCGAGTTTACAATTTTGGCCTCCCAAAACAAGTTGACTCGCAAGCAGAATTGTTTTCCGCACAAACTCAAAAGACTCGCATTTTTTTTTTTTTTGCGAGTTGACAAGTTCGTCAAAAAATTAAAAAAAAGTAAACTTAAAACGCAGCCCTTTCTTTAAATCAAGGGTTGCGAATCATGTCCTTCGCATAAATGAATGATTATTAACTGTTATATGAATGATTAAATGTTCTCGGAGTTATTAACTTATTATACCTAGTAGTACCTCTAGAATAATTATCATTCGGTTCAGGAAGCAAACCGAACTGTTACCACAGTTCAGAAAAATTGAACTGAACCCTTATCGAATTGCCTCGAACCAACTTTTATAGAGATTTGGTGCACCATTTCTGTAGTTTCTAAACCAAACTGGGATTTTTTTAACCAAATTATACTAAGGATAACTAGGACAATTGAAATTAAATAAAAGACCGAACCAAATCAAGTATTAGAAATTAGGACTATTTCAAAAAGACAAAAACCAAATTGCTGAACTGAACTGCAACTATATTTCATAAATTGAACTAAACCGAACTGGTTTCGTATAGTTCAAGAACTGTTAGTCACAGTTTGATTCTTTTTCAATGTTGTTCGTGATACGCCCAATCCTCATCGGGCCAAACCAGCGGCATTGTTACAGAACCTGCAGCATTGTTATGAACTAGTGAAACGTATGAGCTGGTAGTGATAGCTAGAATATTCCCTAGCATTGTTCCTGTGACATGATCTAAAATTATAGGAATAGGCAACAAATCCACTTGTGTAATTAAGCCCACATTTCTTGGTTGAAATAGAAAATTGTATAATATTAAGCGTTTTAGGATGCAATATTGATTGTGGAAAGGTTAAGGACGGTAATTTGTTGGTTAAAAATTTGTGACATAACAAAATTTATTGACGACATTCAGGATGGAAAAAGAAGCTGATTACATTGGGCTTCTATTAGTCGCATCAGCAGGCTATGATCCTCATGTGGCTCCTACGGTCTTAGAGAAATTCATTGATCGTGATTCTATGCTTACAGATTATCGCTCTACTCAACATCGACCTCATATATCTAAAGCTGAAGCCGAGGCAAAATATAGAGCTGTATTGCATGCTCATGCTGAGGAAAAAAAAAGAGCTGAATTGCTCTTTCAAGCTCAGGTAAGAAAAAGAGCCGAATCGCTCGCTAAAGCTCAGGTAATGGATGAAGCTCTTACTATATATGAGAATGTAAGAGCTTGA
Protein sequence

>MS.gene05277.t1

METIPYTNRTHVILVSNAMEKKLGDCVFEKMKADFKNELLPATHPQSVRVGMIANNILDAMKKTLNKETDRSDLGYGSENEGKVEGNWQMEDEKLDDKWVKKGKEQDSQPYTSHLDGLNWEILVVNKPFVDAFCLPGGKIVVFTGLLEHFSSDAEIATFIGREVGHVVARHSAEQFTHNQWFSELRHSSDNNRLSSLFFWRPFGERMEKEADYIGLLLVASAGYDPHVAPTVLEKFIDRDSMLTDYRSTQHRPHISKAEAEAKYRAVLHAHAEEKKRAELLFQAQVRKRAESLAKAQVMDEALTIYENVRA