Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38614.t1 | XP_003602817.2 | 100 | 61 | 0 | 0 | 1 | 61 | 264 | 324 | 1.80E-31 | 144.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38614.t1 | Q9FYF9 | 69.3 | 75 | 14 | 1 | 4 | 78 | 297 | 362 | 4.5e-26 | 118.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38614.t1 | G7J3X3 | 100.0 | 61 | 0 | 0 | 1 | 61 | 264 | 324 | 1.3e-31 | 144.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055818 | MS.gene38614 | 0.82794 | 1.19E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATCAGGTTTAGGTATTC+TGG | 0.262825 | 3.1:+79312383 | None:intergenic |
| TGAGAATGGTGTGGTGGTGA+TGG | 0.367800 | 3.1:+79312581 | MS.gene38614:CDS |
| CTATTGTTCTCGGGCATGTC+AGG | 0.387695 | 3.1:+79312500 | MS.gene38614:CDS |
| AGACACGGCCGCATGAGTTC+CGG | 0.404660 | 3.1:+79312451 | MS.gene38614:CDS |
| TTGAGGCTTTGTGCGCATAT+TGG | 0.412894 | 3.1:+79312414 | MS.gene38614:CDS |
| GGAAGGTTAACTATTGTTCT+CGG | 0.429421 | 3.1:+79312490 | MS.gene38614:CDS |
| GAGAATGGTGTGGTGGTGAT+GGG | 0.434880 | 3.1:+79312582 | MS.gene38614:CDS |
| TTCCGGCGGTGTTCAGTTTG+CGG | 0.442375 | 3.1:+79312468 | MS.gene38614:CDS |
| TCCGGCGGTGTTCAGTTTGC+GGG | 0.443022 | 3.1:+79312469 | MS.gene38614:CDS |
| TTCTCGGGCATGTCAGGCAC+TGG | 0.454108 | 3.1:+79312506 | MS.gene38614:CDS |
| GGTTTAGGTATTCTGGAAGA+TGG | 0.467475 | 3.1:+79312390 | None:intergenic |
| TTGGAAATTGCGGCATAAGA+TGG | 0.479465 | 3.1:+79312530 | MS.gene38614:CDS |
| GTATTCTGGAAGATGGTTTG+AGG | 0.483899 | 3.1:+79312397 | MS.gene38614:CDS |
| ATGGGTTTCGTAGGTGGCGG+CGG | 0.489067 | 3.1:+79312600 | MS.gene38614:CDS |
| GGGCATGTCAGGCACTGGAT+TGG | 0.504850 | 3.1:+79312511 | MS.gene38614:CDS |
| CTTTGTGCGCATATTGGATG+TGG | 0.512732 | 3.1:+79312420 | MS.gene38614:CDS |
| GAAGGTTAACTATTGTTCTC+GGG | 0.514099 | 3.1:+79312491 | MS.gene38614:CDS |
| GTGGTGGTGATGGGTTTCGT+AGG | 0.518779 | 3.1:+79312591 | MS.gene38614:CDS |
| GTGGTGATGGGTTTCGTAGG+TGG | 0.551090 | 3.1:+79312594 | MS.gene38614:CDS |
| GTTGGATGAGAATGGTGTGG+TGG | 0.556948 | 3.1:+79312575 | MS.gene38614:CDS |
| TGCGCATATTGGATGTGGAC+GGG | 0.557752 | 3.1:+79312425 | MS.gene38614:CDS |
| GCGGTGTTCAGTTTGCGGGA+AGG | 0.572429 | 3.1:+79312473 | MS.gene38614:CDS |
| TCCCGCAAACTGAACACCGC+CGG | 0.574784 | 3.1:-79312470 | None:intergenic |
| TAAGATGGAGTGTTCGCCGA+TGG | 0.577189 | 3.1:+79312545 | MS.gene38614:CDS |
| GAATGGTGGTTGGATGAGAA+TGG | 0.581584 | 3.1:+79312567 | MS.gene38614:CDS |
| TTCGCCGATGGAATGGTGGT+TGG | 0.583356 | 3.1:+79312557 | MS.gene38614:CDS |
| TCATCCAACCACCATTCCAT+CGG | 0.583570 | 3.1:-79312561 | None:intergenic |
| GATGTGGACGGGCGGAGACA+CGG | 0.585661 | 3.1:+79312436 | MS.gene38614:CDS |
| TGGAGTGTTCGCCGATGGAA+TGG | 0.597157 | 3.1:+79312550 | MS.gene38614:CDS |
| GTGATGGGTTTCGTAGGTGG+CGG | 0.607351 | 3.1:+79312597 | MS.gene38614:CDS |
| GAACACCGCCGGAACTCATG+CGG | 0.613976 | 3.1:-79312459 | None:intergenic |
| GTGCGCATATTGGATGTGGA+CGG | 0.627782 | 3.1:+79312424 | MS.gene38614:CDS |
| CGGTGATGTGAATGATGCGG+TGG | 0.639416 | 3.1:+79312620 | MS.gene38614:CDS |
| GTGGTTGGATGAGAATGGTG+TGG | 0.639759 | 3.1:+79312572 | MS.gene38614:CDS |
| AGGCACTGGATTGGAAATTG+CGG | 0.642215 | 3.1:+79312520 | MS.gene38614:CDS |
| CACGGCCGCATGAGTTCCGG+CGG | 0.648134 | 3.1:+79312454 | MS.gene38614:CDS |
| AGTGTTCGCCGATGGAATGG+TGG | 0.676530 | 3.1:+79312553 | MS.gene38614:CDS |
| CGGCGGTGATGTGAATGATG+CGG | 0.689320 | 3.1:+79312617 | MS.gene38614:CDS |
| GCATATTGGATGTGGACGGG+CGG | 0.721232 | 3.1:+79312428 | MS.gene38614:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAGGTTAACTATTGTTCTC+GGG | + | chr3.1:79312491-79312510 | MS.gene38614:CDS | 35.0% | |
| GGAAGGTTAACTATTGTTCT+CGG | + | chr3.1:79312490-79312509 | MS.gene38614:CDS | 35.0% | |
| TTGGAAATTGCGGCATAAGA+TGG | + | chr3.1:79312530-79312549 | MS.gene38614:CDS | 40.0% | |
| ! | GTATTCTGGAAGATGGTTTG+AGG | + | chr3.1:79312397-79312416 | MS.gene38614:CDS | 40.0% |
| CTTTGTGCGCATATTGGATG+TGG | + | chr3.1:79312420-79312439 | MS.gene38614:CDS | 45.0% | |
| GAATGGTGGTTGGATGAGAA+TGG | + | chr3.1:79312567-79312586 | MS.gene38614:CDS | 45.0% | |
| TCATCCAACCACCATTCCAT+CGG | - | chr3.1:79312564-79312583 | None:intergenic | 45.0% | |
| ! | TTGAGGCTTTGTGCGCATAT+TGG | + | chr3.1:79312414-79312433 | MS.gene38614:CDS | 45.0% |
| !! | AGGCACTGGATTGGAAATTG+CGG | + | chr3.1:79312520-79312539 | MS.gene38614:CDS | 45.0% |
| CTATTGTTCTCGGGCATGTC+AGG | + | chr3.1:79312500-79312519 | MS.gene38614:CDS | 50.0% | |
| GTGCGCATATTGGATGTGGA+CGG | + | chr3.1:79312424-79312443 | MS.gene38614:CDS | 50.0% | |
| GTGGTTGGATGAGAATGGTG+TGG | + | chr3.1:79312572-79312591 | MS.gene38614:CDS | 50.0% | |
| GTTGGATGAGAATGGTGTGG+TGG | + | chr3.1:79312575-79312594 | MS.gene38614:CDS | 50.0% | |
| TGCGCATATTGGATGTGGAC+GGG | + | chr3.1:79312425-79312444 | MS.gene38614:CDS | 50.0% | |
| ! | GAGAATGGTGTGGTGGTGAT+GGG | + | chr3.1:79312582-79312601 | MS.gene38614:CDS | 50.0% |
| ! | TAAGATGGAGTGTTCGCCGA+TGG | + | chr3.1:79312545-79312564 | MS.gene38614:CDS | 50.0% |
| ! | TGAGAATGGTGTGGTGGTGA+TGG | + | chr3.1:79312581-79312600 | MS.gene38614:CDS | 50.0% |
| AGTGTTCGCCGATGGAATGG+TGG | + | chr3.1:79312553-79312572 | MS.gene38614:CDS | 55.0% | |
| CGGCGGTGATGTGAATGATG+CGG | + | chr3.1:79312617-79312636 | MS.gene38614:CDS | 55.0% | |
| CGGTGATGTGAATGATGCGG+TGG | + | chr3.1:79312620-79312639 | MS.gene38614:CDS | 55.0% | |
| GCATATTGGATGTGGACGGG+CGG | + | chr3.1:79312428-79312447 | MS.gene38614:CDS | 55.0% | |
| TTCCGGCGGTGTTCAGTTTG+CGG | + | chr3.1:79312468-79312487 | MS.gene38614:CDS | 55.0% | |
| TTCGCCGATGGAATGGTGGT+TGG | + | chr3.1:79312557-79312576 | MS.gene38614:CDS | 55.0% | |
| ! | TGGAGTGTTCGCCGATGGAA+TGG | + | chr3.1:79312550-79312569 | MS.gene38614:CDS | 55.0% |
| !! | GTGATGGGTTTCGTAGGTGG+CGG | + | chr3.1:79312597-79312616 | MS.gene38614:CDS | 55.0% |
| !! | GTGGTGATGGGTTTCGTAGG+TGG | + | chr3.1:79312594-79312613 | MS.gene38614:CDS | 55.0% |
| !! | GTGGTGGTGATGGGTTTCGT+AGG | + | chr3.1:79312591-79312610 | MS.gene38614:CDS | 55.0% |
| AGACACGGCCGCATGAGTTC+CGG | + | chr3.1:79312451-79312470 | MS.gene38614:CDS | 60.0% | |
| GAACACCGCCGGAACTCATG+CGG | - | chr3.1:79312462-79312481 | None:intergenic | 60.0% | |
| GCGGTGTTCAGTTTGCGGGA+AGG | + | chr3.1:79312473-79312492 | MS.gene38614:CDS | 60.0% | |
| TCCCGCAAACTGAACACCGC+CGG | - | chr3.1:79312473-79312492 | None:intergenic | 60.0% | |
| TCCGGCGGTGTTCAGTTTGC+GGG | + | chr3.1:79312469-79312488 | MS.gene38614:CDS | 60.0% | |
| TTCTCGGGCATGTCAGGCAC+TGG | + | chr3.1:79312506-79312525 | MS.gene38614:CDS | 60.0% | |
| ! | ATGGGTTTCGTAGGTGGCGG+CGG | + | chr3.1:79312600-79312619 | MS.gene38614:CDS | 60.0% |
| !! | GGGCATGTCAGGCACTGGAT+TGG | + | chr3.1:79312511-79312530 | MS.gene38614:CDS | 60.0% |
| GATGTGGACGGGCGGAGACA+CGG | + | chr3.1:79312436-79312455 | MS.gene38614:CDS | 65.0% | |
| CACGGCCGCATGAGTTCCGG+CGG | + | chr3.1:79312454-79312473 | MS.gene38614:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 79312397 | 79312662 | 79312397 | ID=MS.gene38614 |
| chr3.1 | mRNA | 79312397 | 79312662 | 79312397 | ID=MS.gene38614.t1;Parent=MS.gene38614 |
| chr3.1 | exon | 79312397 | 79312662 | 79312397 | ID=MS.gene38614.t1.exon1;Parent=MS.gene38614.t1 |
| chr3.1 | CDS | 79312397 | 79312662 | 79312397 | ID=cds.MS.gene38614.t1;Parent=MS.gene38614.t1 |
| Gene Sequence |
| Protein sequence |
