Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38663.t1 | ABY48133.1 | 96.8 | 126 | 4 | 0 | 1 | 126 | 1 | 126 | 2.20E-62 | 248.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38663.t1 | Q9LSK9 | 57.5 | 120 | 49 | 2 | 7 | 126 | 8 | 125 | 4.3e-30 | 132.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38663.t1 | G7KF59 | 96.8 | 126 | 4 | 0 | 1 | 126 | 4 | 129 | 1.6e-62 | 248.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050496 | MS.gene38663 | -0.802506 | 5.43E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38663.t1 | MTR_5g030740 | 96.825 | 126 | 4 | 0 | 1 | 126 | 4 | 129 | 5.85e-86 | 246 |
| MS.gene38663.t1 | MTR_8g067590 | 62.609 | 115 | 38 | 3 | 6 | 120 | 7 | 116 | 1.78e-44 | 141 |
| MS.gene38663.t1 | MTR_7g079650 | 81.250 | 48 | 8 | 1 | 79 | 125 | 2 | 49 | 6.42e-21 | 79.7 |
| MS.gene38663.t1 | MTR_7g079600 | 75.385 | 65 | 5 | 1 | 1 | 65 | 3 | 56 | 4.48e-18 | 72.4 |
| MS.gene38663.t1 | MTR_5g067320 | 36.364 | 110 | 67 | 2 | 14 | 120 | 9 | 118 | 3.45e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38663.t1 | AT5G65660 | 57.025 | 121 | 50 | 2 | 6 | 126 | 7 | 125 | 2.92e-41 | 134 |
Find 46 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGACTTGGAAGGTGGGGATT+GGG | 0.296152 | 5.4:-76246925 | None:intergenic |
| TGGACTTGGAAGGTGGGGAT+TGG | 0.315598 | 5.4:-76246926 | None:intergenic |
| CTTGCCCGTGTCAGCATTTG+CGG | 0.335063 | 5.4:+76247323 | MS.gene38663:CDS |
| CGGTGGTGGTCACGGTGGTT+TGG | 0.375927 | 5.4:-76247383 | None:intergenic |
| TATTACCCTGGAGTGAGGTT+TGG | 0.380467 | 5.4:-76246946 | None:intergenic |
| AGTTTAACGGTGTTGATGCC+TGG | 0.390484 | 5.4:+76247271 | MS.gene38663:CDS |
| CTCATCATCTTCAGCTTAAG+TGG | 0.396808 | 5.4:+76246819 | MS.gene38663:CDS |
| TCTTCTCATGTTGCTACCAC+TGG | 0.447898 | 5.4:+76246844 | MS.gene38663:CDS |
| GAGGTTTGGACTTGGAAGGT+GGG | 0.459370 | 5.4:-76246932 | None:intergenic |
| TGAGGTTTGGACTTGGAAGG+TGG | 0.462623 | 5.4:-76246933 | None:intergenic |
| GTTTAACGGTGTTGATGCCT+GGG | 0.471943 | 5.4:+76247272 | MS.gene38663:CDS |
| GACACATCTCGACCGTCTCT+CGG | 0.472213 | 5.4:+76246768 | MS.gene38663:CDS |
| AAGGAAGGGGCAAAGTTTAA+CGG | 0.474374 | 5.4:+76247258 | MS.gene38663:CDS |
| CCTGGAGTGAGGTTTGGACT+TGG | 0.474511 | 5.4:-76246940 | None:intergenic |
| ATTTGAGGTGGGGAAAGAGC+TGG | 0.478175 | 5.4:-76246744 | None:intergenic |
| CCGTCTCTCGGTTTCCCACT+AGG | 0.485458 | 5.4:+76246780 | MS.gene38663:CDS |
| GGTCGAGATGTGTCTATTTG+AGG | 0.498813 | 5.4:-76246759 | None:intergenic |
| CCAAGTCCAAACCTCACTCC+AGG | 0.502297 | 5.4:+76246940 | MS.gene38663:CDS |
| CAAGGCATGGCTATAAACTT+TGG | 0.507163 | 5.4:-76247304 | None:intergenic |
| GGTGTTGATGCCTGGGGATG+AGG | 0.513978 | 5.4:+76247279 | MS.gene38663:CDS |
| CGCAAATGCTGACACGGGCA+AGG | 0.522285 | 5.4:-76247322 | None:intergenic |
| TCAATCTCTCTCTCATCTTG+AGG | 0.522920 | 5.4:+76246887 | MS.gene38663:CDS |
| GAGTGAGGTTTGGACTTGGA+AGG | 0.526857 | 5.4:-76246936 | None:intergenic |
| CGAGATGTGTCTATTTGAGG+TGG | 0.527676 | 5.4:-76246756 | None:intergenic |
| AGCTGAAGATGATGAGCAAG+AGG | 0.534577 | 5.4:-76246812 | None:intergenic |
| AACTTTGGTACCTCATCCCC+AGG | 0.535358 | 5.4:-76247289 | None:intergenic |
| AGAGGAGAGCAGTGCCTAGT+GGG | 0.536628 | 5.4:-76246794 | None:intergenic |
| ATGCTGACACGGGCAAGGCA+TGG | 0.541973 | 5.4:-76247317 | None:intergenic |
| CTTCTCATGTTGCTACCACT+GGG | 0.549662 | 5.4:+76246845 | MS.gene38663:CDS |
| TTTGAGGTGGGGAAAGAGCT+GGG | 0.552650 | 5.4:-76246743 | None:intergenic |
| GGTCACGGTGGTTTGGACGA+TGG | 0.561997 | 5.4:-76247376 | None:intergenic |
| AGGTTTGGACTTGGAAGGTG+GGG | 0.562235 | 5.4:-76246931 | None:intergenic |
| TCTAGCCGCAAATGCTGACA+CGG | 0.568820 | 5.4:-76247328 | None:intergenic |
| GAGATGTGTCTATTTGAGGT+GGG | 0.592610 | 5.4:-76246755 | None:intergenic |
| GAAATTATTACCCTGGAGTG+AGG | 0.601022 | 5.4:-76246951 | None:intergenic |
| TGGTGGATAGGAAACGGAGG+GGG | 0.604236 | 5.4:+76247234 | MS.gene38663:intron |
| AGATGTGTCTATTTGAGGTG+GGG | 0.611401 | 5.4:-76246754 | None:intergenic |
| CAAGTCCAAACCTCACTCCA+GGG | 0.613631 | 5.4:+76246941 | MS.gene38663:CDS |
| CTTGGTGGATAGGAAACGGA+GGG | 0.621199 | 5.4:+76247232 | MS.gene38663:intron |
| AAGAGGAGAGCAGTGCCTAG+TGG | 0.632457 | 5.4:-76246795 | None:intergenic |
| CTAGCCGCAAATGCTGACAC+GGG | 0.647402 | 5.4:-76247327 | None:intergenic |
| CCTAGTGGGAAACCGAGAGA+CGG | 0.673998 | 5.4:-76246780 | None:intergenic |
| GTGGACGGTGGTGGTCACGG+TGG | 0.690653 | 5.4:-76247388 | None:intergenic |
| TCTTGGTGGATAGGAAACGG+AGG | 0.716784 | 5.4:+76247231 | MS.gene38663:intron |
| TTTAACGGTGTTGATGCCTG+GGG | 0.738355 | 5.4:+76247273 | MS.gene38663:CDS |
| TTGGTGGATAGGAAACGGAG+GGG | 0.762221 | 5.4:+76247233 | MS.gene38663:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGATTATAATTTTATCT+TGG | - | chr5.4:76247081-76247100 | None:intergenic | 10.0% |
| !!! | GAATTTACAATTTTGTTTCT+TGG | + | chr5.4:76247214-76247233 | MS.gene38663:intron | 20.0% |
| !!! | TTTACAATTTTGTTTCTTGG+TGG | + | chr5.4:76247217-76247236 | MS.gene38663:intron | 25.0% |
| AAAGAGTGAAATTATTACCC+TGG | - | chr5.4:76246961-76246980 | None:intergenic | 30.0% | |
| AGGATCATACAGTTGTAAAA+TGG | - | chr5.4:76247120-76247139 | None:intergenic | 30.0% | |
| TTGACGTTGTAAGACAAAAA+AGG | - | chr5.4:76247140-76247159 | None:intergenic | 30.0% | |
| !! | AATTTTGTTTCTTGGTGGAT+AGG | + | chr5.4:76247222-76247241 | MS.gene38663:intron | 30.0% |
| !!! | TTTTTTGTCTTACAACGTCA+AGG | + | chr5.4:76247139-76247158 | MS.gene38663:intron | 30.0% |
| AAGGAAGGGGCAAAGTTTAA+CGG | + | chr5.4:76247258-76247277 | MS.gene38663:CDS | 40.0% | |
| CAAGGCATGGCTATAAACTT+TGG | - | chr5.4:76247307-76247326 | None:intergenic | 40.0% | |
| CTCATCATCTTCAGCTTAAG+TGG | + | chr5.4:76246819-76246838 | MS.gene38663:CDS | 40.0% | |
| GAAATTATTACCCTGGAGTG+AGG | - | chr5.4:76246954-76246973 | None:intergenic | 40.0% | |
| TCAATCTCTCTCTCATCTTG+AGG | + | chr5.4:76246887-76246906 | MS.gene38663:CDS | 40.0% | |
| ! | AGATGTGTCTATTTGAGGTG+GGG | - | chr5.4:76246757-76246776 | None:intergenic | 40.0% |
| ! | GAGATGTGTCTATTTGAGGT+GGG | - | chr5.4:76246758-76246777 | None:intergenic | 40.0% |
| ! | GTTTCTTGGTGGATAGGAAA+CGG | + | chr5.4:76247228-76247247 | MS.gene38663:intron | 40.0% |
| AGCTGAAGATGATGAGCAAG+AGG | - | chr5.4:76246815-76246834 | None:intergenic | 45.0% | |
| CGAGATGTGTCTATTTGAGG+TGG | - | chr5.4:76246759-76246778 | None:intergenic | 45.0% | |
| CTTCTCATGTTGCTACCACT+GGG | + | chr5.4:76246845-76246864 | MS.gene38663:CDS | 45.0% | |
| GGTCGAGATGTGTCTATTTG+AGG | - | chr5.4:76246762-76246781 | None:intergenic | 45.0% | |
| TATTACCCTGGAGTGAGGTT+TGG | - | chr5.4:76246949-76246968 | None:intergenic | 45.0% | |
| TCTTCTCATGTTGCTACCAC+TGG | + | chr5.4:76246844-76246863 | MS.gene38663:CDS | 45.0% | |
| ! | GTGAACGAAACTTTTCCCAG+TGG | - | chr5.4:76246863-76246882 | None:intergenic | 45.0% |
| !! | AGTTTAACGGTGTTGATGCC+TGG | + | chr5.4:76247271-76247290 | MS.gene38663:CDS | 45.0% |
| !! | GTTTAACGGTGTTGATGCCT+GGG | + | chr5.4:76247272-76247291 | MS.gene38663:CDS | 45.0% |
| !! | TTTAACGGTGTTGATGCCTG+GGG | + | chr5.4:76247273-76247292 | MS.gene38663:CDS | 45.0% |
| !!! | ATAATTTGAAATTATAAAAT+TGG | + | chr5.4:76247023-76247042 | MS.gene38663:intron | 5.0% |
| AACTTTGGTACCTCATCCCC+AGG | - | chr5.4:76247292-76247311 | None:intergenic | 50.0% | |
| AGGTTTGGACTTGGAAGGTG+GGG | - | chr5.4:76246934-76246953 | None:intergenic | 50.0% | |
| CAAGTCCAAACCTCACTCCA+GGG | + | chr5.4:76246941-76246960 | MS.gene38663:CDS | 50.0% | |
| GAGGTTTGGACTTGGAAGGT+GGG | - | chr5.4:76246935-76246954 | None:intergenic | 50.0% | |
| GAGTGAGGTTTGGACTTGGA+AGG | - | chr5.4:76246939-76246958 | None:intergenic | 50.0% | |
| TCTAGCCGCAAATGCTGACA+CGG | - | chr5.4:76247331-76247350 | None:intergenic | 50.0% | |
| TGAGGTTTGGACTTGGAAGG+TGG | - | chr5.4:76246936-76246955 | None:intergenic | 50.0% | |
| ! | ATTTGAGGTGGGGAAAGAGC+TGG | - | chr5.4:76246747-76246766 | None:intergenic | 50.0% |
| ! | CTTGGTGGATAGGAAACGGA+GGG | + | chr5.4:76247232-76247251 | MS.gene38663:intron | 50.0% |
| ! | TCTTGGTGGATAGGAAACGG+AGG | + | chr5.4:76247231-76247250 | MS.gene38663:intron | 50.0% |
| ! | TTGGTGGATAGGAAACGGAG+GGG | + | chr5.4:76247233-76247252 | MS.gene38663:intron | 50.0% |
| ! | TTTGAGGTGGGGAAAGAGCT+GGG | - | chr5.4:76246746-76246765 | None:intergenic | 50.0% |
| AAACGGAGGGGGAAAGGAAG+GGG | + | chr5.4:76247245-76247264 | MS.gene38663:CDS | 55.0% | |
| AAGAGGAGAGCAGTGCCTAG+TGG | - | chr5.4:76246798-76246817 | None:intergenic | 55.0% | |
| AGAGGAGAGCAGTGCCTAGT+GGG | - | chr5.4:76246797-76246816 | None:intergenic | 55.0% | |
| CCAAGTCCAAACCTCACTCC+AGG | + | chr5.4:76246940-76246959 | MS.gene38663:CDS | 55.0% | |
| CCTAGTGGGAAACCGAGAGA+CGG | - | chr5.4:76246783-76246802 | None:intergenic | 55.0% | |
| CCTGGAGTGAGGTTTGGACT+TGG | - | chr5.4:76246943-76246962 | None:intergenic | 55.0% | |
| CTAGCCGCAAATGCTGACAC+GGG | - | chr5.4:76247330-76247349 | None:intergenic | 55.0% | |
| CTTGCCCGTGTCAGCATTTG+CGG | + | chr5.4:76247323-76247342 | MS.gene38663:CDS | 55.0% | |
| GAAACGGAGGGGGAAAGGAA+GGG | + | chr5.4:76247244-76247263 | MS.gene38663:CDS | 55.0% | |
| GACACATCTCGACCGTCTCT+CGG | + | chr5.4:76246768-76246787 | MS.gene38663:CDS | 55.0% | |
| GATAGGAAACGGAGGGGGAA+AGG | + | chr5.4:76247239-76247258 | MS.gene38663:intron | 55.0% | |
| GGACTTGGAAGGTGGGGATT+GGG | - | chr5.4:76246928-76246947 | None:intergenic | 55.0% | |
| TGGACTTGGAAGGTGGGGAT+TGG | - | chr5.4:76246929-76246948 | None:intergenic | 55.0% | |
| ! | TGGTGGATAGGAAACGGAGG+GGG | + | chr5.4:76247234-76247253 | MS.gene38663:intron | 55.0% |
| ATGCTGACACGGGCAAGGCA+TGG | - | chr5.4:76247320-76247339 | None:intergenic | 60.0% | |
| CCGTCTCTCGGTTTCCCACT+AGG | + | chr5.4:76246780-76246799 | MS.gene38663:CDS | 60.0% | |
| CGCAAATGCTGACACGGGCA+AGG | - | chr5.4:76247325-76247344 | None:intergenic | 60.0% | |
| GGAAACGGAGGGGGAAAGGA+AGG | + | chr5.4:76247243-76247262 | MS.gene38663:intron | 60.0% | |
| !! | GGTGTTGATGCCTGGGGATG+AGG | + | chr5.4:76247279-76247298 | MS.gene38663:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 76246735 | 76247396 | 76246735 | ID=MS.gene38663 |
| chr5.4 | mRNA | 76246735 | 76247396 | 76246735 | ID=MS.gene38663.t1;Parent=MS.gene38663 |
| chr5.4 | exon | 76246735 | 76246962 | 76246735 | ID=MS.gene38663.t1.exon1;Parent=MS.gene38663.t1 |
| chr5.4 | CDS | 76246735 | 76246962 | 76246735 | ID=cds.MS.gene38663.t1;Parent=MS.gene38663.t1 |
| chr5.4 | exon | 76247244 | 76247396 | 76247244 | ID=MS.gene38663.t1.exon2;Parent=MS.gene38663.t1 |
| chr5.4 | CDS | 76247244 | 76247396 | 76247244 | ID=cds.MS.gene38663.t1;Parent=MS.gene38663.t1 |
| Gene Sequence |
| Protein sequence |
