Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38665.t1 | ABY48133.1 | 96.8 | 126 | 4 | 0 | 1 | 126 | 1 | 126 | 2.20E-62 | 248.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38665.t1 | Q9LSK9 | 57.5 | 120 | 49 | 2 | 7 | 126 | 8 | 125 | 4.3e-30 | 132.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38665.t1 | G7KF59 | 96.8 | 126 | 4 | 0 | 1 | 126 | 4 | 129 | 1.6e-62 | 248.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050496 | MS.gene38665 | -0.802252 | 6.13E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38665.t1 | MTR_5g030740 | 96.825 | 126 | 4 | 0 | 1 | 126 | 4 | 129 | 5.85e-86 | 246 |
| MS.gene38665.t1 | MTR_8g067590 | 62.609 | 115 | 38 | 3 | 6 | 120 | 7 | 116 | 1.78e-44 | 141 |
| MS.gene38665.t1 | MTR_7g079650 | 81.250 | 48 | 8 | 1 | 79 | 125 | 2 | 49 | 6.42e-21 | 79.7 |
| MS.gene38665.t1 | MTR_7g079600 | 75.385 | 65 | 5 | 1 | 1 | 65 | 3 | 56 | 4.48e-18 | 72.4 |
| MS.gene38665.t1 | MTR_5g067320 | 36.364 | 110 | 67 | 2 | 14 | 120 | 9 | 118 | 3.45e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38665.t1 | AT5G65660 | 57.025 | 121 | 50 | 2 | 6 | 126 | 7 | 125 | 2.92e-41 | 134 |
Find 46 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGACTTGGAAGGTGGGGATT+GGG | 0.296152 | 5.1:-20984635 | None:intergenic |
| TGGACTTGGAAGGTGGGGAT+TGG | 0.315598 | 5.1:-20984636 | None:intergenic |
| CTTGCCCGTGTCAGCATTTG+CGG | 0.335063 | 5.1:+20985033 | MS.gene38665:CDS |
| CGGTGGTGGTCACGGTGGTT+TGG | 0.375927 | 5.1:-20985093 | None:intergenic |
| TATTACCCTGGAGTGAGGTT+TGG | 0.380467 | 5.1:-20984656 | None:intergenic |
| AGTTTAACGGTGTTGATGCC+TGG | 0.390484 | 5.1:+20984981 | MS.gene38665:CDS |
| CTCATCATCTTCAGCTTAAG+TGG | 0.396808 | 5.1:+20984529 | MS.gene38665:CDS |
| TCTTCTCATGTTGCTACCAC+TGG | 0.447898 | 5.1:+20984554 | MS.gene38665:CDS |
| GAGGTTTGGACTTGGAAGGT+GGG | 0.459370 | 5.1:-20984642 | None:intergenic |
| TGAGGTTTGGACTTGGAAGG+TGG | 0.462623 | 5.1:-20984643 | None:intergenic |
| GTTTAACGGTGTTGATGCCT+GGG | 0.471943 | 5.1:+20984982 | MS.gene38665:CDS |
| GACACATCTCGACCGTCTCT+CGG | 0.472213 | 5.1:+20984478 | MS.gene38665:CDS |
| AAGGAAGGGGCAAAGTTTAA+CGG | 0.474374 | 5.1:+20984968 | MS.gene38665:CDS |
| CCTGGAGTGAGGTTTGGACT+TGG | 0.474511 | 5.1:-20984650 | None:intergenic |
| ATTTGAGGTGGGGAAAGAGC+TGG | 0.478175 | 5.1:-20984454 | None:intergenic |
| CCGTCTCTCGGTTTCCCACT+AGG | 0.485458 | 5.1:+20984490 | MS.gene38665:CDS |
| GGTCGAGATGTGTCTATTTG+AGG | 0.498813 | 5.1:-20984469 | None:intergenic |
| CCAAGTCCAAACCTCACTCC+AGG | 0.502297 | 5.1:+20984650 | MS.gene38665:CDS |
| CAAGGCATGGCTATAAACTT+TGG | 0.507163 | 5.1:-20985014 | None:intergenic |
| GGTGTTGATGCCTGGGGATG+AGG | 0.513978 | 5.1:+20984989 | MS.gene38665:CDS |
| CGCAAATGCTGACACGGGCA+AGG | 0.522285 | 5.1:-20985032 | None:intergenic |
| TCAATCTCTCTCTCATCTTG+AGG | 0.522920 | 5.1:+20984597 | MS.gene38665:CDS |
| GAGTGAGGTTTGGACTTGGA+AGG | 0.526857 | 5.1:-20984646 | None:intergenic |
| CGAGATGTGTCTATTTGAGG+TGG | 0.527676 | 5.1:-20984466 | None:intergenic |
| AGCTGAAGATGATGAGCAAG+AGG | 0.534577 | 5.1:-20984522 | None:intergenic |
| AACTTTGGTACCTCATCCCC+AGG | 0.535358 | 5.1:-20984999 | None:intergenic |
| AGAGGAGAGCAGTGCCTAGT+GGG | 0.536628 | 5.1:-20984504 | None:intergenic |
| ATGCTGACACGGGCAAGGCA+TGG | 0.541973 | 5.1:-20985027 | None:intergenic |
| CTTCTCATGTTGCTACCACT+GGG | 0.549662 | 5.1:+20984555 | MS.gene38665:CDS |
| TTTGAGGTGGGGAAAGAGCT+GGG | 0.552650 | 5.1:-20984453 | None:intergenic |
| GGTCACGGTGGTTTGGACGA+TGG | 0.561997 | 5.1:-20985086 | None:intergenic |
| AGGTTTGGACTTGGAAGGTG+GGG | 0.562235 | 5.1:-20984641 | None:intergenic |
| TCTAGCCGCAAATGCTGACA+CGG | 0.568820 | 5.1:-20985038 | None:intergenic |
| GAGATGTGTCTATTTGAGGT+GGG | 0.592610 | 5.1:-20984465 | None:intergenic |
| GAAATTATTACCCTGGAGTG+AGG | 0.601022 | 5.1:-20984661 | None:intergenic |
| TGGTGGATAGGAAACGGAGG+GGG | 0.604236 | 5.1:+20984944 | MS.gene38665:intron |
| AGATGTGTCTATTTGAGGTG+GGG | 0.611401 | 5.1:-20984464 | None:intergenic |
| CAAGTCCAAACCTCACTCCA+GGG | 0.613631 | 5.1:+20984651 | MS.gene38665:CDS |
| CTTGGTGGATAGGAAACGGA+GGG | 0.621199 | 5.1:+20984942 | MS.gene38665:intron |
| AAGAGGAGAGCAGTGCCTAG+TGG | 0.632457 | 5.1:-20984505 | None:intergenic |
| CTAGCCGCAAATGCTGACAC+GGG | 0.647402 | 5.1:-20985037 | None:intergenic |
| CCTAGTGGGAAACCGAGAGA+CGG | 0.673998 | 5.1:-20984490 | None:intergenic |
| GTGGACGGTGGTGGTCACGG+TGG | 0.690653 | 5.1:-20985098 | None:intergenic |
| TCTTGGTGGATAGGAAACGG+AGG | 0.716784 | 5.1:+20984941 | MS.gene38665:intron |
| TTTAACGGTGTTGATGCCTG+GGG | 0.738355 | 5.1:+20984983 | MS.gene38665:CDS |
| TTGGTGGATAGGAAACGGAG+GGG | 0.762221 | 5.1:+20984943 | MS.gene38665:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGATTATAATTTTATCT+TGG | - | chr5.1:20984791-20984810 | None:intergenic | 10.0% |
| !!! | GAATTTACAATTTTGTTTCT+TGG | + | chr5.1:20984924-20984943 | MS.gene38665:intron | 20.0% |
| !!! | TTTACAATTTTGTTTCTTGG+TGG | + | chr5.1:20984927-20984946 | MS.gene38665:intron | 25.0% |
| AAAGAGTGAAATTATTACCC+TGG | - | chr5.1:20984671-20984690 | None:intergenic | 30.0% | |
| AGGATCATACAGTTGTAAAA+TGG | - | chr5.1:20984830-20984849 | None:intergenic | 30.0% | |
| TTGACGTTGTAAGACAAAAA+AGG | - | chr5.1:20984850-20984869 | None:intergenic | 30.0% | |
| !! | AATTTTGTTTCTTGGTGGAT+AGG | + | chr5.1:20984932-20984951 | MS.gene38665:intron | 30.0% |
| !!! | TTTTTTGTCTTACAACGTCA+AGG | + | chr5.1:20984849-20984868 | MS.gene38665:intron | 30.0% |
| AAGGAAGGGGCAAAGTTTAA+CGG | + | chr5.1:20984968-20984987 | MS.gene38665:CDS | 40.0% | |
| CAAGGCATGGCTATAAACTT+TGG | - | chr5.1:20985017-20985036 | None:intergenic | 40.0% | |
| CTCATCATCTTCAGCTTAAG+TGG | + | chr5.1:20984529-20984548 | MS.gene38665:CDS | 40.0% | |
| GAAATTATTACCCTGGAGTG+AGG | - | chr5.1:20984664-20984683 | None:intergenic | 40.0% | |
| TCAATCTCTCTCTCATCTTG+AGG | + | chr5.1:20984597-20984616 | MS.gene38665:CDS | 40.0% | |
| ! | AGATGTGTCTATTTGAGGTG+GGG | - | chr5.1:20984467-20984486 | None:intergenic | 40.0% |
| ! | GAGATGTGTCTATTTGAGGT+GGG | - | chr5.1:20984468-20984487 | None:intergenic | 40.0% |
| ! | GTTTCTTGGTGGATAGGAAA+CGG | + | chr5.1:20984938-20984957 | MS.gene38665:intron | 40.0% |
| AGCTGAAGATGATGAGCAAG+AGG | - | chr5.1:20984525-20984544 | None:intergenic | 45.0% | |
| CGAGATGTGTCTATTTGAGG+TGG | - | chr5.1:20984469-20984488 | None:intergenic | 45.0% | |
| CTTCTCATGTTGCTACCACT+GGG | + | chr5.1:20984555-20984574 | MS.gene38665:CDS | 45.0% | |
| GGTCGAGATGTGTCTATTTG+AGG | - | chr5.1:20984472-20984491 | None:intergenic | 45.0% | |
| TATTACCCTGGAGTGAGGTT+TGG | - | chr5.1:20984659-20984678 | None:intergenic | 45.0% | |
| TCTTCTCATGTTGCTACCAC+TGG | + | chr5.1:20984554-20984573 | MS.gene38665:CDS | 45.0% | |
| ! | GTGAACGAAACTTTTCCCAG+TGG | - | chr5.1:20984573-20984592 | None:intergenic | 45.0% |
| !! | AGTTTAACGGTGTTGATGCC+TGG | + | chr5.1:20984981-20985000 | MS.gene38665:CDS | 45.0% |
| !! | GTTTAACGGTGTTGATGCCT+GGG | + | chr5.1:20984982-20985001 | MS.gene38665:CDS | 45.0% |
| !! | TTTAACGGTGTTGATGCCTG+GGG | + | chr5.1:20984983-20985002 | MS.gene38665:CDS | 45.0% |
| !!! | ATAATTTGAAATTATAAAAT+TGG | + | chr5.1:20984733-20984752 | MS.gene38665:intron | 5.0% |
| AACTTTGGTACCTCATCCCC+AGG | - | chr5.1:20985002-20985021 | None:intergenic | 50.0% | |
| AGGTTTGGACTTGGAAGGTG+GGG | - | chr5.1:20984644-20984663 | None:intergenic | 50.0% | |
| CAAGTCCAAACCTCACTCCA+GGG | + | chr5.1:20984651-20984670 | MS.gene38665:CDS | 50.0% | |
| GAGGTTTGGACTTGGAAGGT+GGG | - | chr5.1:20984645-20984664 | None:intergenic | 50.0% | |
| GAGTGAGGTTTGGACTTGGA+AGG | - | chr5.1:20984649-20984668 | None:intergenic | 50.0% | |
| TCTAGCCGCAAATGCTGACA+CGG | - | chr5.1:20985041-20985060 | None:intergenic | 50.0% | |
| TGAGGTTTGGACTTGGAAGG+TGG | - | chr5.1:20984646-20984665 | None:intergenic | 50.0% | |
| ! | ATTTGAGGTGGGGAAAGAGC+TGG | - | chr5.1:20984457-20984476 | None:intergenic | 50.0% |
| ! | CTTGGTGGATAGGAAACGGA+GGG | + | chr5.1:20984942-20984961 | MS.gene38665:intron | 50.0% |
| ! | TCTTGGTGGATAGGAAACGG+AGG | + | chr5.1:20984941-20984960 | MS.gene38665:intron | 50.0% |
| ! | TTGGTGGATAGGAAACGGAG+GGG | + | chr5.1:20984943-20984962 | MS.gene38665:intron | 50.0% |
| ! | TTTGAGGTGGGGAAAGAGCT+GGG | - | chr5.1:20984456-20984475 | None:intergenic | 50.0% |
| AAACGGAGGGGGAAAGGAAG+GGG | + | chr5.1:20984955-20984974 | MS.gene38665:CDS | 55.0% | |
| AAGAGGAGAGCAGTGCCTAG+TGG | - | chr5.1:20984508-20984527 | None:intergenic | 55.0% | |
| AGAGGAGAGCAGTGCCTAGT+GGG | - | chr5.1:20984507-20984526 | None:intergenic | 55.0% | |
| CCAAGTCCAAACCTCACTCC+AGG | + | chr5.1:20984650-20984669 | MS.gene38665:CDS | 55.0% | |
| CCTAGTGGGAAACCGAGAGA+CGG | - | chr5.1:20984493-20984512 | None:intergenic | 55.0% | |
| CCTGGAGTGAGGTTTGGACT+TGG | - | chr5.1:20984653-20984672 | None:intergenic | 55.0% | |
| CTAGCCGCAAATGCTGACAC+GGG | - | chr5.1:20985040-20985059 | None:intergenic | 55.0% | |
| CTTGCCCGTGTCAGCATTTG+CGG | + | chr5.1:20985033-20985052 | MS.gene38665:CDS | 55.0% | |
| GAAACGGAGGGGGAAAGGAA+GGG | + | chr5.1:20984954-20984973 | MS.gene38665:CDS | 55.0% | |
| GACACATCTCGACCGTCTCT+CGG | + | chr5.1:20984478-20984497 | MS.gene38665:CDS | 55.0% | |
| GATAGGAAACGGAGGGGGAA+AGG | + | chr5.1:20984949-20984968 | MS.gene38665:intron | 55.0% | |
| GGACTTGGAAGGTGGGGATT+GGG | - | chr5.1:20984638-20984657 | None:intergenic | 55.0% | |
| TGGACTTGGAAGGTGGGGAT+TGG | - | chr5.1:20984639-20984658 | None:intergenic | 55.0% | |
| ! | TGGTGGATAGGAAACGGAGG+GGG | + | chr5.1:20984944-20984963 | MS.gene38665:intron | 55.0% |
| ATGCTGACACGGGCAAGGCA+TGG | - | chr5.1:20985030-20985049 | None:intergenic | 60.0% | |
| CCGTCTCTCGGTTTCCCACT+AGG | + | chr5.1:20984490-20984509 | MS.gene38665:CDS | 60.0% | |
| CGCAAATGCTGACACGGGCA+AGG | - | chr5.1:20985035-20985054 | None:intergenic | 60.0% | |
| GGAAACGGAGGGGGAAAGGA+AGG | + | chr5.1:20984953-20984972 | MS.gene38665:intron | 60.0% | |
| !! | GGTGTTGATGCCTGGGGATG+AGG | + | chr5.1:20984989-20985008 | MS.gene38665:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 20984445 | 20985106 | 20984445 | ID=MS.gene38665 |
| chr5.1 | mRNA | 20984445 | 20985106 | 20984445 | ID=MS.gene38665.t1;Parent=MS.gene38665 |
| chr5.1 | exon | 20984445 | 20984672 | 20984445 | ID=MS.gene38665.t1.exon1;Parent=MS.gene38665.t1 |
| chr5.1 | CDS | 20984445 | 20984672 | 20984445 | ID=cds.MS.gene38665.t1;Parent=MS.gene38665.t1 |
| chr5.1 | exon | 20984954 | 20985106 | 20984954 | ID=MS.gene38665.t1.exon2;Parent=MS.gene38665.t1 |
| chr5.1 | CDS | 20984954 | 20985106 | 20984954 | ID=cds.MS.gene38665.t1;Parent=MS.gene38665.t1 |
| Gene Sequence |
| Protein sequence |
