AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39137

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39137.t1 MTR_7g090230 96.037 429 11 3 1 426 1 426 0.0 841
MS.gene39137.t1 MTR_1g051180 52.955 440 118 11 1 426 1 365 5.23e-133 387
MS.gene39137.t1 MTR_8g038440 65.806 155 51 1 273 425 101 255 4.20e-69 219
MS.gene39137.t1 MTR_3g017490 63.158 152 55 1 273 424 110 260 9.54e-68 217
MS.gene39137.t1 MTR_7g104720 34.184 392 209 12 75 425 22 405 1.23e-49 173
MS.gene39137.t1 MTR_3g465550 47.305 167 84 3 256 420 64 228 2.53e-48 165
MS.gene39137.t1 MTR_5g083110 51.592 157 73 3 268 424 85 238 1.67e-47 163
MS.gene39137.t1 MTR_5g072400 40.284 211 116 4 221 424 46 253 1.04e-45 159
MS.gene39137.t1 MTR_6g011640 28.767 438 193 14 64 425 3 397 2.18e-43 157
MS.gene39137.t1 MTR_7g010090 31.092 357 162 13 90 425 139 432 2.05e-39 147
MS.gene39137.t1 MTR_7g106500 44.375 160 79 2 274 425 122 279 2.15e-38 140
MS.gene39137.t1 MTR_3g071510 43.085 188 98 6 239 424 54 234 2.71e-38 139
MS.gene39137.t1 MTR_1g090400 43.046 151 83 2 274 424 221 368 5.78e-37 139
MS.gene39137.t1 MTR_8g099665 39.130 161 84 3 274 424 211 367 6.81e-33 127
MS.gene39137.t1 MTR_8g106900 41.026 156 86 4 274 424 119 273 9.75e-31 119
MS.gene39137.t1 MTR_3g030520 38.608 158 80 3 285 425 142 299 1.96e-26 108
MS.gene39137.t1 MTR_5g048870 40.230 87 50 1 279 363 70 156 6.84e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39137.t1 AT2G41330 50.000 390 154 10 53 425 36 401 1.84e-112 336
MS.gene39137.t1 AT3G57070 48.737 396 174 8 48 425 32 416 4.78e-112 335
MS.gene39137.t1 AT5G13810 64.162 173 60 2 254 426 104 274 4.33e-72 228
MS.gene39137.t1 AT5G39865 31.842 380 219 13 64 425 32 389 5.28e-48 169
MS.gene39137.t1 AT5G58530 48.125 160 73 3 273 424 108 265 7.59e-45 157
MS.gene39137.t1 AT5G03870 31.472 394 190 14 76 424 25 383 7.00e-40 147
MS.gene39137.t1 AT5G06470 41.772 158 81 3 274 424 85 238 4.47e-37 135
MS.gene39137.t1 AT4G10630 31.165 369 173 14 75 424 27 333 9.29e-37 137
MS.gene39137.t1 AT3G28850 41.477 176 74 3 279 425 252 427 1.17e-36 139
MS.gene39137.t1 AT1G64500 41.772 158 85 2 274 424 208 365 7.01e-36 135
MS.gene39137.t1 AT1G32760 42.197 173 92 4 258 424 143 313 1.49e-33 128
MS.gene39137.t1 AT3G11773 42.143 140 77 3 285 424 14 149 1.02e-32 121
MS.gene39137.t1 AT5G01420 38.816 152 89 3 274 424 252 400 2.53e-30 121
MS.gene39137.t1 AT3G11773 40.179 112 63 3 313 424 92 199 2.98e-22 94.4

Find 55 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGTTTCTGTTAGCAACTA+TGG 0.271791 7.4:-18638936 None:intergenic
TTTCAATGGATTCATCTTAT+AGG 0.287479 7.4:+18639854 MS.gene39137:CDS
TGTGTTCATTGTAATGAAAA+TGG 0.321593 7.4:+18640135 MS.gene39137:CDS
TGATCATGAGGGGTAAAGTT+AGG 0.340853 7.4:-18639193 None:intergenic
TTGAAACAATTGAATGAATC+TGG 0.363003 7.4:+18639967 MS.gene39137:CDS
AGAAGCTTTGCTTGCTAAGT+TGG 0.365047 7.4:+18639546 MS.gene39137:CDS
TGTTTATCTGAGAAACTCAT+TGG 0.370099 7.4:-18639367 None:intergenic
AAGGAGTTGCAGAATGCATT+TGG 0.393748 7.4:+18639877 MS.gene39137:CDS
AAAGAAGAACAAGGATTCAT+AGG 0.402640 7.4:-18639679 None:intergenic
GATGAATAAGAGGCATTGTA+AGG 0.415383 7.4:-18639081 None:intergenic
AAAGAAACAGAAGGTGTTGT+TGG 0.422488 7.4:-18638965 None:intergenic
TTTCAGATGTGTAAGGTCAA+TGG 0.435956 7.4:+18639648 MS.gene39137:CDS
TCACTCACTTCCACTACCTA+TGG 0.439023 7.4:+18639142 MS.gene39137:CDS
GTGAGATGGGGTTGATCATG+AGG 0.444128 7.4:-18639205 None:intergenic
GTTGGAGGGTGATGAATAAG+AGG 0.449999 7.4:-18639091 None:intergenic
GTAATAATTGCAATGGTAGC+AGG 0.462789 7.4:+18640076 MS.gene39137:CDS
CTGTAACTCCAAGCAACACT+TGG 0.466501 7.4:-18639571 None:intergenic
AAGAACAAGGTAAATTGAAA+AGG 0.483693 7.4:+18640112 MS.gene39137:CDS
CATCAACACTTGGGAACTCA+TGG 0.487477 7.4:+18639273 MS.gene39137:CDS
AGTTGGATCCAAGTGTTGCT+TGG 0.490779 7.4:+18639563 MS.gene39137:CDS
CTACCATTGCAATTATTACA+AGG 0.490905 7.4:-18640072 None:intergenic
CAAGTGTTGATGACAGAATC+TGG 0.492141 7.4:-18639262 None:intergenic
CAGATTCTGTCATCAACACT+TGG 0.496185 7.4:+18639263 MS.gene39137:CDS
TTGACCTTACACATCTGAAA+AGG 0.497664 7.4:-18639645 None:intergenic
GAGAAGAGAACCATAGGTAG+TGG 0.505781 7.4:-18639152 None:intergenic
GATCATGAGGGGTAAAGTTA+GGG 0.512539 7.4:-18639192 None:intergenic
TGGTAGCAGGAAAGTGTTTG+AGG 0.513533 7.4:+18640089 MS.gene39137:CDS
AATGGATTCATCTTATAGGA+AGG 0.527593 7.4:+18639858 MS.gene39137:CDS
TGAGATGGGGTTGATCATGA+GGG 0.532269 7.4:-18639204 None:intergenic
TGATGAAAGAGATATTTCAA+TGG 0.533526 7.4:+18639840 MS.gene39137:CDS
AATTAGGAGAAGAGAACCAT+AGG 0.536814 7.4:-18639158 None:intergenic
ATCAGTAGTGACATTGCCAC+AGG 0.554763 7.4:+18639906 MS.gene39137:CDS
AAAATCTCAAGAAACCACTT+TGG 0.555258 7.4:+18639509 MS.gene39137:CDS
GAAGATTGAAAAGAAACAGA+AGG 0.555642 7.4:-18638974 None:intergenic
TCTGATAAATGCTGCCAAAG+TGG 0.561325 7.4:-18639523 None:intergenic
TAGTGGAAGTGAGTGAAACA+AGG 0.572068 7.4:-18639135 None:intergenic
ACAAACCAAGGATCTTGAGT+TGG 0.577028 7.4:-18640018 None:intergenic
CAGATAAACACAGTTCATGC+AGG 0.580945 7.4:+18639380 MS.gene39137:CDS
GAGTTGCAGAATGCATTTGG+TGG 0.586689 7.4:+18639880 MS.gene39137:CDS
GAATTAGCTAGAATGTTGAA+AGG 0.590447 7.4:+18639991 MS.gene39137:CDS
AACACTTGGGAACTCATGGA+TGG 0.595857 7.4:+18639277 MS.gene39137:CDS
TGGTTTGTTTGTGACAAATG+TGG 0.596999 7.4:+18640033 MS.gene39137:CDS
TTTATCAGAGGGAAGCATGT+GGG 0.599499 7.4:+18639931 MS.gene39137:CDS
GAAATCTTCAGAAACAGTGA+TGG 0.600720 7.4:+18639459 MS.gene39137:CDS
GTGCCTTGTAATAATTGCAA+TGG 0.616885 7.4:+18640069 MS.gene39137:CDS
ATGAATAAGAGGCATTGTAA+GGG 0.620378 7.4:-18639080 None:intergenic
GCATTGTCATCAAGACAACT+AGG 0.624676 7.4:+18639598 MS.gene39137:CDS
GTTTATTGCACAAGTTTGCG+AGG 0.649260 7.4:+18639751 MS.gene39137:CDS
AGATTCTGTCATCAACACTT+GGG 0.657274 7.4:+18639264 MS.gene39137:CDS
CATTTGTCACAAACAAACCA+AGG 0.669189 7.4:-18640030 None:intergenic
TTATCAAACAAAGAAGAACA+AGG 0.689315 7.4:-18639688 None:intergenic
GAGATGGGGTTGATCATGAG+GGG 0.692266 7.4:-18639203 None:intergenic
ATGAAGATTGTTGTGCAGTG+AGG 0.704749 7.4:+18639788 MS.gene39137:CDS
AAAGTGTTTGAGGAAGAACA+AGG 0.708450 7.4:+18640099 MS.gene39137:CDS
TTGTCATCAAGACAACTAGG+TGG 0.763609 7.4:+18639601 MS.gene39137:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGAACAAGGTAAATTGAAA+AGG + chr7.4:18640112-18640131 MS.gene39137:CDS 25.0%
! TGATGAAAGAGATATTTCAA+TGG + chr7.4:18639840-18639859 MS.gene39137:CDS 25.0%
! TGTGTTCATTGTAATGAAAA+TGG + chr7.4:18640135-18640154 MS.gene39137:CDS 25.0%
! TTATCAAACAAAGAAGAACA+AGG - chr7.4:18639691-18639710 None:intergenic 25.0%
! TTGAAACAATTGAATGAATC+TGG + chr7.4:18639967-18639986 MS.gene39137:CDS 25.0%
!! TTTCAATGGATTCATCTTAT+AGG + chr7.4:18639854-18639873 MS.gene39137:CDS 25.0%
!!! TGGTTTCTTGAGATTTTTTT+TGG - chr7.4:18639506-18639525 None:intergenic 25.0%
!!! TTCTTCATTTTGAGAAGTTT+TGG - chr7.4:18639230-18639249 None:intergenic 25.0%
AAAATCTCAAGAAACCACTT+TGG + chr7.4:18639509-18639528 MS.gene39137:CDS 30.0%
AAAGAAGAACAAGGATTCAT+AGG - chr7.4:18639682-18639701 None:intergenic 30.0%
ACTCATTGGATTGTCAAAAA+AGG - chr7.4:18639356-18639375 None:intergenic 30.0%
ATTGGATTGTCAAAAAAGGA+AGG - chr7.4:18639352-18639371 None:intergenic 30.0%
CTACCATTGCAATTATTACA+AGG - chr7.4:18640075-18640094 None:intergenic 30.0%
GAAGATTGAAAAGAAACAGA+AGG - chr7.4:18638977-18638996 None:intergenic 30.0%
GAATTAGCTAGAATGTTGAA+AGG + chr7.4:18639991-18640010 MS.gene39137:CDS 30.0%
TGTTTATCTGAGAAACTCAT+TGG - chr7.4:18639370-18639389 None:intergenic 30.0%
! AATGGATTCATCTTATAGGA+AGG + chr7.4:18639858-18639877 MS.gene39137:CDS 30.0%
!! ATGAATAAGAGGCATTGTAA+GGG - chr7.4:18639083-18639102 None:intergenic 30.0%
!!! AACTATGGTTGTTGTTTTTG+AGG - chr7.4:18638924-18638943 None:intergenic 30.0%
AATTAGGAGAAGAGAACCAT+AGG - chr7.4:18639161-18639180 None:intergenic 35.0%
AGATTCTGTCATCAACACTT+GGG + chr7.4:18639264-18639283 MS.gene39137:CDS 35.0%
CATTTGTCACAAACAAACCA+AGG - chr7.4:18640033-18640052 None:intergenic 35.0%
GAAATCTTCAGAAACAGTGA+TGG + chr7.4:18639459-18639478 MS.gene39137:CDS 35.0%
GTAATAATTGCAATGGTAGC+AGG + chr7.4:18640076-18640095 MS.gene39137:CDS 35.0%
GTGCCTTGTAATAATTGCAA+TGG + chr7.4:18640069-18640088 MS.gene39137:CDS 35.0%
TTGACCTTACACATCTGAAA+AGG - chr7.4:18639648-18639667 None:intergenic 35.0%
TTTCAGATGTGTAAGGTCAA+TGG + chr7.4:18639648-18639667 MS.gene39137:CDS 35.0%
! AAAGTGTTTGAGGAAGAACA+AGG + chr7.4:18640099-18640118 MS.gene39137:CDS 35.0%
! AAGAGTTTGTGTCTTTTACC+TGG + chr7.4:18639709-18639728 MS.gene39137:CDS 35.0%
! ACTATTCTGTTTTCAGTACC+AGG - chr7.4:18639730-18639749 None:intergenic 35.0%
! AGAACCTTTTCAGATGTGTA+AGG + chr7.4:18639641-18639660 MS.gene39137:CDS 35.0%
! TGTGTTTCTGTTAGCAACTA+TGG - chr7.4:18638939-18638958 None:intergenic 35.0%
!! AAAGAAACAGAAGGTGTTGT+TGG - chr7.4:18638968-18638987 None:intergenic 35.0%
!! GATGAATAAGAGGCATTGTA+AGG - chr7.4:18639084-18639103 None:intergenic 35.0%
!! TAGGGACTTTTTGGTCAATT+AGG - chr7.4:18639177-18639196 None:intergenic 35.0%
!! TGGTTTGTTTGTGACAAATG+TGG + chr7.4:18640033-18640052 MS.gene39137:CDS 35.0%
!! TTGAGAAGTTTTGGTGAGAT+GGG - chr7.4:18639221-18639240 None:intergenic 35.0%
!! TTTGAGAAGTTTTGGTGAGA+TGG - chr7.4:18639222-18639241 None:intergenic 35.0%
AAGGAGTTGCAGAATGCATT+TGG + chr7.4:18639877-18639896 MS.gene39137:CDS 40.0%
ACAAACCAAGGATCTTGAGT+TGG - chr7.4:18640021-18640040 None:intergenic 40.0%
ATGAAGATTGTTGTGCAGTG+AGG + chr7.4:18639788-18639807 MS.gene39137:CDS 40.0%
CAGATAAACACAGTTCATGC+AGG + chr7.4:18639380-18639399 MS.gene39137:CDS 40.0%
CAGATTCTGTCATCAACACT+TGG + chr7.4:18639263-18639282 MS.gene39137:CDS 40.0%
GATCATGAGGGGTAAAGTTA+GGG - chr7.4:18639195-18639214 None:intergenic 40.0%
GCATTGTCATCAAGACAACT+AGG + chr7.4:18639598-18639617 MS.gene39137:CDS 40.0%
GTTTATTGCACAAGTTTGCG+AGG + chr7.4:18639751-18639770 MS.gene39137:CDS 40.0%
TAGTGGAAGTGAGTGAAACA+AGG - chr7.4:18639138-18639157 None:intergenic 40.0%
TCTGATAAATGCTGCCAAAG+TGG - chr7.4:18639526-18639545 None:intergenic 40.0%
TGATCATGAGGGGTAAAGTT+AGG - chr7.4:18639196-18639215 None:intergenic 40.0%
TTCCCTCTGATAAAAACCTG+TGG - chr7.4:18639925-18639944 None:intergenic 40.0%
TTGTCATCAAGACAACTAGG+TGG + chr7.4:18639601-18639620 MS.gene39137:CDS 40.0%
! AGAAGCTTTGCTTGCTAAGT+TGG + chr7.4:18639546-18639565 MS.gene39137:CDS 40.0%
! AGGGACAAAGCTTTTCTAAC+TGG - chr7.4:18639064-18639083 None:intergenic 40.0%
! GACAAAGCTTTTCTAACTGG+TGG - chr7.4:18639061-18639080 None:intergenic 40.0%
! GGGTAAAGTTAGGGACTTTT+TGG - chr7.4:18639186-18639205 None:intergenic 40.0%
! GTTTTCCAACTCAAGATCCT+TGG + chr7.4:18640013-18640032 MS.gene39137:CDS 40.0%
! TGCAGGTACACAACTTTTGA+TGG + chr7.4:18639397-18639416 MS.gene39137:CDS 40.0%
! TGCAGTGAGGATGATTTTGA+GGG + chr7.4:18639801-18639820 MS.gene39137:CDS 40.0%
! TGCCACAGGTTTTTATCAGA+GGG + chr7.4:18639920-18639939 MS.gene39137:CDS 40.0%
! TTGCCACAGGTTTTTATCAG+AGG + chr7.4:18639919-18639938 MS.gene39137:CDS 40.0%
! TTTATCAGAGGGAAGCATGT+GGG + chr7.4:18639931-18639950 MS.gene39137:CDS 40.0%
!! CAAGTGTTGATGACAGAATC+TGG - chr7.4:18639265-18639284 None:intergenic 40.0%
!! TGAGAAGTTTTGGTGAGATG+GGG - chr7.4:18639220-18639239 None:intergenic 40.0%
!! TTTTATCAGAGGGAAGCATG+TGG + chr7.4:18639930-18639949 MS.gene39137:CDS 40.0%
!!! GTGATGAGTGTCACCTTTTT+TGG - chr7.4:18639116-18639135 None:intergenic 40.0%
!!! TGAGTGTCACCTTTTTTGGT+TGG - chr7.4:18639112-18639131 None:intergenic 40.0%
!!! TGTCACCTTTTTTGGTTGGA+GGG - chr7.4:18639108-18639127 None:intergenic 40.0%
AACACTTGGGAACTCATGGA+TGG + chr7.4:18639277-18639296 MS.gene39137:CDS 45.0%
ATCAGTAGTGACATTGCCAC+AGG + chr7.4:18639906-18639925 MS.gene39137:CDS 45.0%
CATCAACACTTGGGAACTCA+TGG + chr7.4:18639273-18639292 MS.gene39137:CDS 45.0%
CATCACCCTCCAACCAAAAA+AGG + chr7.4:18639100-18639119 MS.gene39137:CDS 45.0%
CTGTAACTCCAAGCAACACT+TGG - chr7.4:18639574-18639593 None:intergenic 45.0%
GAGAAGAGAACCATAGGTAG+TGG - chr7.4:18639155-18639174 None:intergenic 45.0%
GAGTTGCAGAATGCATTTGG+TGG + chr7.4:18639880-18639899 MS.gene39137:CDS 45.0%
GTTGGAGGGTGATGAATAAG+AGG - chr7.4:18639094-18639113 None:intergenic 45.0%
TCACTCACTTCCACTACCTA+TGG + chr7.4:18639142-18639161 MS.gene39137:CDS 45.0%
! AGTTGGATCCAAGTGTTGCT+TGG + chr7.4:18639563-18639582 MS.gene39137:CDS 45.0%
! GCAGTGAGGATGATTTTGAG+GGG + chr7.4:18639802-18639821 MS.gene39137:CDS 45.0%
! GTGCAGTGAGGATGATTTTG+AGG + chr7.4:18639800-18639819 MS.gene39137:CDS 45.0%
! TGAGATGGGGTTGATCATGA+GGG - chr7.4:18639207-18639226 None:intergenic 45.0%
!! TGGTAGCAGGAAAGTGTTTG+AGG + chr7.4:18640089-18640108 MS.gene39137:CDS 45.0%
!!! GTGTCACCTTTTTTGGTTGG+AGG - chr7.4:18639109-18639128 None:intergenic 45.0%
! GAGATGGGGTTGATCATGAG+GGG - chr7.4:18639206-18639225 None:intergenic 50.0%
! GTGAGATGGGGTTGATCATG+AGG - chr7.4:18639208-18639227 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 18638908 18640188 18638908 ID=MS.gene39137
chr7.4 mRNA 18638908 18640188 18638908 ID=MS.gene39137.t1;Parent=MS.gene39137
chr7.4 exon 18638908 18640188 18638908 ID=MS.gene39137.t1.exon1;Parent=MS.gene39137.t1
chr7.4 CDS 18638908 18640188 18638908 ID=cds.MS.gene39137.t1;Parent=MS.gene39137.t1
Gene Sequence

>MS.gene39137

ATGGGTTGTTCAGCCTCAAAAACAACAACCATAGTTGCTAACAGAAACACAGAAGATCCAACAACACCTTCTGTTTCTTTTCAATCTTCATCTTCATCAACTTCTTCATCTTTTTCATCTTCTTATGCTTCAAACTACTTCAATTCTTCACCACCAGTTAGAAAAGCTTTGTCCCTTACAATGCCTCTTATTCATCACCCTCCAACCAAAAAAGGTGACACTCATCACCTTGTTTCACTCACTTCCACTACCTATGGTTCTCTTCTCCTAATTGACCAAAAAGTCCCTAACTTTACCCCTCATGATCAACCCCATCTCACCAAAACTTCTCAAAATGAAGAACAATCTTTGTCACCAGATTCTGTCATCAACACTTGGGAACTCATGGATGGTTTAGATGAACATGAAGATTCTCATCATGATAATACTAATGTTCATAAACCTTCCTTTTTTGACAATCCAATGAGTTTCTCAGATAAACACAGTTCATGCAGGTACACAACTTTTGATGGATCAGCAAAAAAGAAACTTCTTGATTCATTTGAATCATTGAAATCTTCAGAAACAGTGATGGAAGAAAGAAATTCTTCTTCTTCCAAAAAAAATCTCAAGAAACCACTTTGGCAGCATTTATCAGAAGAAGCTTTGCTTGCTAAGTTGGATCCAAGTGTTGCTTGGAGTTACAGAAGAGCATTGTCATCAAGACAACTAGGTGGTAGCAACAACAATAATAAGAACCTTTTCAGATGTGTAAGGTCAATGGAATCAAGTCCTATGAATCCTTGTTCTTCTTTGTTTGATAAGAGTTTGTGTCTTTTACCTGGTACTGAAAACAGAATAGTTGTTTATTGCACAAGTTTGCGAGGAATTCGAAAGACTTATGAAGATTGTTGTGCAGTGAGGATGATTTTGAGGGGATATAGAGTTGCAGTTGATGAAAGAGATATTTCAATGGATTCATCTTATAGGAAGGAGTTGCAGAATGCATTTGGTGGCAAATCAGTAGTGACATTGCCACAGGTTTTTATCAGAGGGAAGCATGTGGGAAATGCAGATGATTTGAAACAATTGAATGAATCTGGTGAATTAGCTAGAATGTTGAAAGGTTTTCCAACTCAAGATCCTTGGTTTGTTTGTGACAAATGTGGTGATGCAAGATTTGTGCCTTGTAATAATTGCAATGGTAGCAGGAAAGTGTTTGAGGAAGAACAAGGTAAATTGAAAAGGTGTGTTCATTGTAATGAAAATGGATTGATTAGATGCTCAAGTTGTTGTTCATGA
Protein sequence

>MS.gene39137.t1

MGCSASKTTTIVANRNTEDPTTPSVSFQSSSSSTSSSFSSSYASNYFNSSPPVRKALSLTMPLIHHPPTKKGDTHHLVSLTSTTYGSLLLIDQKVPNFTPHDQPHLTKTSQNEEQSLSPDSVINTWELMDGLDEHEDSHHDNTNVHKPSFFDNPMSFSDKHSSCRYTTFDGSAKKKLLDSFESLKSSETVMEERNSSSSKKNLKKPLWQHLSEEALLAKLDPSVAWSYRRALSSRQLGGSNNNNKNLFRCVRSMESSPMNPCSSLFDKSLCLLPGTENRIVVYCTSLRGIRKTYEDCCAVRMILRGYRVAVDERDISMDSSYRKELQNAFGGKSVVTLPQVFIRGKHVGNADDLKQLNESGELARMLKGFPTQDPWFVCDKCGDARFVPCNNCNGSRKVFEEEQGKLKRCVHCNENGLIRCSSCCS