Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39850.t1 | XP_013443258.1 | 79.5 | 254 | 28 | 4 | 118 | 367 | 36 | 269 | 4.00E-108 | 401.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39850.t1 | P23472 | 55.4 | 258 | 85 | 6 | 118 | 367 | 35 | 270 | 2.3e-72 | 274.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39850.t1 | Q6XD74 | 79.5 | 254 | 28 | 4 | 118 | 367 | 36 | 269 | 2.9e-108 | 401.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene39850 | 0.816862 | 4.44E-52 | -1.69E-46 |
| MS.gene060165 | MS.gene39850 | 0.809136 | 2.20E-50 | -1.69E-46 |
| MS.gene060619 | MS.gene39850 | 0.800111 | 1.68E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39850.t1 | MTR_0027s0260 | 73.835 | 279 | 49 | 4 | 93 | 367 | 11 | 269 | 6.85e-137 | 394 |
| MS.gene39850.t1 | MTR_8g055940 | 67.832 | 286 | 67 | 5 | 86 | 367 | 4 | 268 | 2.79e-124 | 362 |
| MS.gene39850.t1 | MTR_5g043550 | 62.950 | 278 | 78 | 5 | 94 | 367 | 9 | 265 | 8.46e-112 | 330 |
| MS.gene39850.t1 | MTR_8g467650 | 62.977 | 262 | 71 | 4 | 112 | 367 | 28 | 269 | 7.64e-103 | 307 |
| MS.gene39850.t1 | MTR_1g099320 | 56.693 | 254 | 88 | 7 | 118 | 367 | 33 | 268 | 1.72e-85 | 263 |
| MS.gene39850.t1 | MTR_7g116350 | 58.333 | 252 | 73 | 5 | 118 | 367 | 34 | 255 | 7.11e-84 | 273 |
| MS.gene39850.t1 | MTR_1g099310 | 54.118 | 255 | 91 | 7 | 118 | 367 | 34 | 267 | 2.05e-81 | 252 |
| MS.gene39850.t1 | MTR_0027s0250 | 100.000 | 118 | 0 | 0 | 1 | 118 | 1 | 118 | 3.37e-81 | 245 |
| MS.gene39850.t1 | MTR_5g087380 | 100.000 | 118 | 0 | 0 | 1 | 118 | 1 | 118 | 3.37e-81 | 245 |
| MS.gene39850.t1 | MTR_1g099350 | 51.190 | 252 | 101 | 5 | 118 | 367 | 31 | 262 | 1.01e-78 | 245 |
| MS.gene39850.t1 | MTR_1g099290 | 51.056 | 284 | 103 | 8 | 92 | 367 | 14 | 269 | 8.16e-77 | 249 |
| MS.gene39850.t1 | MTR_2g102020 | 45.161 | 279 | 119 | 5 | 104 | 368 | 27 | 285 | 7.03e-75 | 237 |
| MS.gene39850.t1 | MTR_2g040740 | 47.876 | 259 | 103 | 8 | 118 | 367 | 35 | 270 | 7.84e-65 | 211 |
| MS.gene39850.t1 | MTR_7g116850 | 45.977 | 261 | 122 | 5 | 111 | 367 | 52 | 297 | 3.14e-64 | 209 |
| MS.gene39850.t1 | MTR_2g040830 | 46.154 | 260 | 107 | 9 | 118 | 367 | 35 | 271 | 1.33e-57 | 192 |
| MS.gene39850.t1 | MTR_2g040690 | 44.615 | 260 | 111 | 9 | 118 | 367 | 37 | 273 | 3.17e-56 | 188 |
| MS.gene39850.t1 | MTR_2g040650 | 40.769 | 260 | 117 | 9 | 118 | 367 | 35 | 267 | 1.07e-47 | 166 |
| MS.gene39850.t1 | MTR_6g023340 | 36.260 | 262 | 146 | 8 | 111 | 367 | 28 | 273 | 1.11e-44 | 157 |
| MS.gene39850.t1 | MTR_2g040870 | 45.217 | 115 | 62 | 1 | 101 | 215 | 15 | 128 | 1.39e-23 | 97.1 |
| MS.gene39850.t1 | MTR_2g040880 | 52.273 | 88 | 38 | 3 | 284 | 367 | 1 | 88 | 1.42e-22 | 93.6 |
| MS.gene39850.t1 | MTR_2g040850 | 46.875 | 96 | 49 | 1 | 118 | 213 | 35 | 128 | 1.27e-19 | 87.8 |
| MS.gene39850.t1 | MTR_2g040890 | 44.340 | 106 | 55 | 3 | 111 | 213 | 28 | 132 | 5.71e-18 | 82.0 |
| MS.gene39850.t1 | MTR_2g040900 | 42.105 | 95 | 50 | 4 | 277 | 366 | 17 | 111 | 2.40e-13 | 68.2 |
| MS.gene39850.t1 | MTR_4g034720 | 35.870 | 92 | 53 | 2 | 3 | 89 | 14 | 104 | 8.17e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39850.t1 | AT5G24090 | 52.174 | 253 | 101 | 5 | 118 | 367 | 38 | 273 | 5.65e-81 | 251 |
| MS.gene39850.t1 | AT1G16740 | 85.593 | 118 | 17 | 0 | 1 | 118 | 1 | 118 | 7.10e-70 | 216 |
| MS.gene39850.t1 | ATCG00660 | 39.326 | 89 | 52 | 1 | 3 | 89 | 14 | 102 | 6.04e-14 | 68.6 |
Find 113 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCCCATTATACCCTTTA+AGG | 0.085833 | 5.4:-68709572 | None:intergenic |
| TCAAGTTGGCAAAGGGGTTC+AGG | 0.250503 | 5.4:+68702757 | MS.gene39850:CDS |
| GATCTTGCCAGGTACCTTAA+AGG | 0.268374 | 5.4:+68709558 | MS.gene39850:CDS |
| CAGGCCTCTTTGATTATGTT+TGG | 0.268573 | 5.4:+68709661 | MS.gene39850:CDS |
| CACCGTGCTTCCAGCTATTA+AGG | 0.272724 | 5.4:+68709857 | MS.gene39850:CDS |
| CTCTAGAAGGACACCTTTCT+TGG | 0.287315 | 5.4:-68704676 | None:intergenic |
| CAGCAACACTTTGATGCCTT+TGG | 0.313438 | 5.4:-68706002 | None:intergenic |
| TTTGGCTTTGTTTGCGAGTT+GGG | 0.313573 | 5.4:+68706096 | MS.gene39850:CDS |
| TTTGTTTGCGAGTTGGGTTT+TGG | 0.319052 | 5.4:+68709881 | MS.gene39850:CDS |
| CCCAAGAATATCTTGTTTGC+AGG | 0.325190 | 5.4:-68709774 | None:intergenic |
| GGAGGGGAAGGAAAGGCTTA+TGG | 0.329819 | 5.4:+68709912 | MS.gene39850:CDS |
| CCCTGCAAACAAGATATTCT+TGG | 0.331404 | 5.4:+68709773 | MS.gene39850:CDS |
| AGGTACCTTAAAGGGTATAA+TGG | 0.340472 | 5.4:+68709567 | MS.gene39850:CDS |
| ACTGCTTCCATGCATCTTCA+AGG | 0.357026 | 5.4:-68709737 | None:intergenic |
| TTTATATGCAGGTCAATTAT+GGG | 0.359592 | 5.4:+68704506 | MS.gene39850:intron |
| AGGCGTTGCAGTATTCTTAC+AGG | 0.360205 | 5.4:+68702826 | MS.gene39850:CDS |
| TCTCCAGAGGCTGCAGGCAG+TGG | 0.360681 | 5.4:+68709807 | MS.gene39850:CDS |
| AAGTGTAGCAACTTACCTTT+TGG | 0.364632 | 5.4:+68706078 | MS.gene39850:CDS |
| ACAACCACATTCTTGTTTCC+AGG | 0.370533 | 5.4:-68704649 | None:intergenic |
| CCTGCAAACAAGATATTCTT+GGG | 0.370588 | 5.4:+68709774 | MS.gene39850:CDS |
| ACCCTATAGTTTCAAGTCAT+TGG | 0.373887 | 5.4:+68704603 | MS.gene39850:CDS |
| AAGCCACTGCCTGCAGCCTC+TGG | 0.380345 | 5.4:-68709810 | None:intergenic |
| TTTGTTTGCGAGTTGGGTTT+TGG | 0.381125 | 5.4:+68706102 | MS.gene39850:CDS |
| AGGCCTCTTTGATTATGTTT+GGG | 0.384986 | 5.4:+68709662 | MS.gene39850:CDS |
| TACCAATGACTTGAAACTAT+AGG | 0.394532 | 5.4:-68704605 | None:intergenic |
| AGAGGTAAACCCTTGTTTGC+GGG | 0.398127 | 5.4:+68709935 | MS.gene39850:CDS |
| GAAGAGCAAAGAATTGCATT+AGG | 0.408094 | 5.4:+68702781 | MS.gene39850:CDS |
| ACCAATGACTTGAAACTATA+GGG | 0.411529 | 5.4:-68704604 | None:intergenic |
| TTAGCCGAGGCTTGTGCCAC+AGG | 0.416958 | 5.4:+68705842 | MS.gene39850:CDS |
| GGTACCTTAAAGGGTATAAT+GGG | 0.421587 | 5.4:+68709568 | MS.gene39850:CDS |
| AATGCTGGCACCCGCATTCA+TGG | 0.423259 | 5.4:+68702900 | MS.gene39850:CDS |
| AGTGTCCATTTCCTGATGCT+TGG | 0.425947 | 5.4:+68709619 | MS.gene39850:CDS |
| CAAGTTGGCAAAGGGGTTCA+GGG | 0.428217 | 5.4:+68702758 | MS.gene39850:CDS |
| ATATCACGGAATGCATTTCC+TGG | 0.451719 | 5.4:+68704631 | MS.gene39850:CDS |
| AGGAGGATCAAACCAACATT+GGG | 0.452709 | 5.4:+68709534 | MS.gene39850:intron |
| CCTATCCAAGCATCAGGAAA+TGG | 0.452807 | 5.4:-68709624 | None:intergenic |
| TGCTCGGGAGAGGGTGGAGA+AGG | 0.459767 | 5.4:+68702806 | MS.gene39850:CDS |
| CCTGCTTCTCCAGAGGCTGC+AGG | 0.461719 | 5.4:+68709801 | MS.gene39850:CDS |
| TTGGAGGGGAAGGGAGGGGA+AGG | 0.467495 | 5.4:+68706121 | MS.gene39850:CDS |
| ATCTTGCCAGGTACCTTAAA+GGG | 0.472734 | 5.4:+68709559 | MS.gene39850:CDS |
| GCAAACAAAACCTTAATAGC+TGG | 0.475085 | 5.4:-68709867 | None:intergenic |
| ATAGCCTTCTTACCAACCTT+CGG | 0.476060 | 5.4:+68705884 | MS.gene39850:CDS |
| GCATTTCCTATCCAAGCATC+AGG | 0.480978 | 5.4:-68709630 | None:intergenic |
| CCAGTGCATTCGTTACTGTA+TGG | 0.481353 | 5.4:-68705950 | None:intergenic |
| CCTGCAGCCTCTGGAGAAGC+AGG | 0.482734 | 5.4:-68709801 | None:intergenic |
| CTTCATGCATGGGCTGTTGA+AGG | 0.484005 | 5.4:+68704531 | MS.gene39850:CDS |
| GGGGAAGGGAGGGGAAGGAA+AGG | 0.484825 | 5.4:+68706126 | MS.gene39850:CDS |
| AGAATTGCATTAGGATTGCT+CGG | 0.485950 | 5.4:+68702790 | MS.gene39850:CDS |
| GAATATTCAACTTAACAGAA+AGG | 0.486572 | 5.4:+68704555 | MS.gene39850:CDS |
| TTCTTACCAACCTTCGGTGA+TGG | 0.490452 | 5.4:+68705890 | MS.gene39850:CDS |
| CTGTCATTAGGAGGAGGTGC+TGG | 0.492751 | 5.4:+68706022 | MS.gene39850:CDS |
| ATCACCGAAGGTTGGTAAGA+AGG | 0.497166 | 5.4:-68705888 | None:intergenic |
| GCATTAGGATTGCTCGGGAG+AGG | 0.500972 | 5.4:+68702796 | MS.gene39850:CDS |
| ATAGGAAATGCACTTACAAC+AGG | 0.501034 | 5.4:+68709642 | MS.gene39850:CDS |
| ATCAAAGTGTTGCTGTCATT+AGG | 0.502542 | 5.4:+68706010 | MS.gene39850:CDS |
| TTGGAGGGGAATGGAGGGGA+AGG | 0.505470 | 5.4:+68709900 | MS.gene39850:CDS |
| TGCGATCACTTTGGATCCAA+CGG | 0.506932 | 5.4:+68702874 | MS.gene39850:CDS |
| GGGGAATGGAGGGGAAGGAA+AGG | 0.515328 | 5.4:+68709905 | MS.gene39850:CDS |
| TAGAAGGACACCTTTCTTGG+AGG | 0.516509 | 5.4:-68704673 | None:intergenic |
| AAGGAGGATCAAACCAACAT+TGG | 0.522229 | 5.4:+68709533 | MS.gene39850:intron |
| TGGATCCAACGGATTAATGC+TGG | 0.525665 | 5.4:+68702885 | MS.gene39850:CDS |
| CCTCCTTGTCAATACAATCC+TGG | 0.526414 | 5.4:+68709702 | MS.gene39850:CDS |
| CCATTTCCTGATGCTTGGAT+AGG | 0.527986 | 5.4:+68709624 | MS.gene39850:CDS |
| AGAGGGACATGCGATCACTT+TGG | 0.533930 | 5.4:+68702865 | MS.gene39850:CDS |
| ATTATACCCTTTAAGGTACC+TGG | 0.538948 | 5.4:-68709565 | None:intergenic |
| CATTAGGATTGCTCGGGAGA+GGG | 0.540793 | 5.4:+68702797 | MS.gene39850:CDS |
| AACTCGCAAACAAAGCCAAA+AGG | 0.541054 | 5.4:-68706093 | None:intergenic |
| TGTGGTTGTCCCTCCAAGAA+AGG | 0.544481 | 5.4:+68704663 | MS.gene39850:CDS |
| CCAAGAATATCTTGTTTGCA+GGG | 0.546890 | 5.4:-68709773 | None:intergenic |
| GAGAGGTAAACCCTTGTTTG+CGG | 0.552743 | 5.4:+68709934 | MS.gene39850:CDS |
| AGAAGGACACCTTTCTTGGA+GGG | 0.553759 | 5.4:-68704672 | None:intergenic |
| GGGGTTACCTGCTTCTCCAG+AGG | 0.565316 | 5.4:+68709794 | MS.gene39850:CDS |
| AATTATGGGAACTTCATGCA+TGG | 0.565534 | 5.4:+68704520 | MS.gene39850:CDS |
| TCAAGGTTGCTAATTTCACC+AGG | 0.566959 | 5.4:-68709720 | None:intergenic |
| TTAGCAACCTTGAAGATGCA+TGG | 0.569798 | 5.4:+68709730 | MS.gene39850:CDS |
| ACTCGTATGATCAATCTAGC+TGG | 0.572207 | 5.4:+68705917 | MS.gene39850:CDS |
| TAGGATTGCTCGGGAGAGGG+TGG | 0.573081 | 5.4:+68702800 | MS.gene39850:CDS |
| AAGACATACACCATGAATGC+GGG | 0.576838 | 5.4:-68702910 | None:intergenic |
| TTGAAGATGCATGGAAGCAG+TGG | 0.579167 | 5.4:+68709739 | MS.gene39850:CDS |
| AGTCATTGGTAGACATATCA+CGG | 0.592754 | 5.4:+68704617 | MS.gene39850:CDS |
| AGTATTCTTACAGGGATCGC+CGG | 0.595705 | 5.4:+68702835 | MS.gene39850:CDS |
| GTTTGTCCATCACCGAAGGT+TGG | 0.597533 | 5.4:-68705896 | None:intergenic |
| AACCTTAATAGCTGGAAGCA+CGG | 0.599739 | 5.4:-68709859 | None:intergenic |
| ATCAAATCATGTCAAGCCAA+AGG | 0.602180 | 5.4:+68705986 | MS.gene39850:CDS |
| AAGTTGGCAAAGGGGTTCAG+GGG | 0.603918 | 5.4:+68702759 | MS.gene39850:CDS |
| TCACCAGGATTGTATTGACA+AGG | 0.607450 | 5.4:-68709705 | None:intergenic |
| TGGGGCCAGAATGGCAACGA+GGG | 0.608155 | 5.4:+68705815 | MS.gene39850:intron |
| GAATTGCATTAGGATTGCTC+GGG | 0.608963 | 5.4:+68702791 | MS.gene39850:CDS |
| ATTATGGGAACTTCATGCAT+GGG | 0.609578 | 5.4:+68704521 | MS.gene39850:CDS |
| AAAGTGTTGCTGTCATTAGG+AGG | 0.610534 | 5.4:+68706013 | MS.gene39850:CDS |
| CCATACAGTAACGAATGCAC+TGG | 0.610802 | 5.4:+68705950 | MS.gene39850:CDS |
| GTGGAAGTAAGATCAGCTGC+AGG | 0.610892 | 5.4:-68709837 | None:intergenic |
| GTACCCAAACATAATCAAAG+AGG | 0.611062 | 5.4:-68709665 | None:intergenic |
| GTTCCCTGTGGCACAAGCCT+CGG | 0.614415 | 5.4:-68705846 | None:intergenic |
| GGCGTTGCAGTATTCTTACA+GGG | 0.617032 | 5.4:+68702827 | MS.gene39850:CDS |
| CAACGAGGGCACGTTAGCCG+AGG | 0.622463 | 5.4:+68705829 | MS.gene39850:CDS |
| ACGAGTTTGTCCATCACCGA+AGG | 0.623456 | 5.4:-68705900 | None:intergenic |
| GTGTTGCTGTCATTAGGAGG+AGG | 0.625745 | 5.4:+68706016 | MS.gene39850:CDS |
| TAGCCGAGGCTTGTGCCACA+GGG | 0.629033 | 5.4:+68705843 | MS.gene39850:CDS |
| GGAAGGAAAGGCTTATGGAG+AGG | 0.631688 | 5.4:+68709917 | MS.gene39850:CDS |
| CCAGGATTGTATTGACAAGG+AGG | 0.644665 | 5.4:-68709702 | None:intergenic |
| CAGGATTGTATTGACAAGGA+GGG | 0.646372 | 5.4:-68709701 | None:intergenic |
| GGGTGCCAGCATTAATCCGT+TGG | 0.658550 | 5.4:-68702890 | None:intergenic |
| CTGCAAACAAGATATTCTTG+GGG | 0.663277 | 5.4:+68709775 | MS.gene39850:CDS |
| GGAGGATCAAACCAACATTG+GGG | 0.672908 | 5.4:+68709535 | MS.gene39850:intron |
| GAAGACATACACCATGAATG+CGG | 0.682942 | 5.4:-68702911 | None:intergenic |
| GGAAGGAAAGGCTTATGCAG+AGG | 0.683409 | 5.4:+68706138 | MS.gene39850:CDS |
| CACATATTCATAGTTCCCTG+TGG | 0.709745 | 5.4:-68705858 | None:intergenic |
| CTTAATAGCTGGAAGCACGG+TGG | 0.709815 | 5.4:-68709856 | None:intergenic |
| GCATCAGGAAATGGACACTG+AGG | 0.711554 | 5.4:-68709615 | None:intergenic |
| GAGGATCAAACCAACATTGG+GGG | 0.711566 | 5.4:+68709536 | MS.gene39850:CDS |
| TTGGGGCCAGAATGGCAACG+AGG | 0.717322 | 5.4:+68705814 | MS.gene39850:intron |
| ATTTCCTGGAAACAAGAATG+TGG | 0.721015 | 5.4:+68704645 | MS.gene39850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 68702738 | 68710019 | 68702738 | ID=MS.gene39850 |
| chr5.4 | mRNA | 68702738 | 68710019 | 68702738 | ID=MS.gene39850.t1;Parent=MS.gene39850 |
| chr5.4 | exon | 68702738 | 68702923 | 68702738 | ID=MS.gene39850.t1.exon1;Parent=MS.gene39850.t1 |
| chr5.4 | CDS | 68702738 | 68702923 | 68702738 | ID=cds.MS.gene39850.t1;Parent=MS.gene39850.t1 |
| chr5.4 | exon | 68704517 | 68704684 | 68704517 | ID=MS.gene39850.t1.exon2;Parent=MS.gene39850.t1 |
| chr5.4 | CDS | 68704517 | 68704684 | 68704517 | ID=cds.MS.gene39850.t1;Parent=MS.gene39850.t1 |
| chr5.4 | exon | 68705824 | 68706227 | 68705824 | ID=MS.gene39850.t1.exon3;Parent=MS.gene39850.t1 |
| chr5.4 | CDS | 68705824 | 68706227 | 68705824 | ID=cds.MS.gene39850.t1;Parent=MS.gene39850.t1 |
| chr5.4 | exon | 68709536 | 68710019 | 68709536 | ID=MS.gene39850.t1.exon4;Parent=MS.gene39850.t1 |
| chr5.4 | CDS | 68709536 | 68710019 | 68709536 | ID=cds.MS.gene39850.t1;Parent=MS.gene39850.t1 |
| Gene Sequence |
| Protein sequence |
