Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40090.t1 | XP_003608349.1 | 95.7 | 164 | 7 | 0 | 1 | 164 | 1 | 164 | 4.80E-81 | 310.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40090.t1 | Q9LXA7 | 34.7 | 196 | 94 | 6 | 1 | 164 | 1 | 194 | 9.0e-12 | 71.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40090.t1 | A0A072UP94 | 95.7 | 164 | 7 | 0 | 1 | 164 | 1 | 164 | 3.5e-81 | 310.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050681 | MS.gene40090 | 0.802726 | 4.89E-49 | -1.69E-46 |
| MS.gene053618 | MS.gene40090 | 0.829666 | 4.53E-55 | -1.69E-46 |
| MS.gene053620 | MS.gene40090 | 0.804984 | 1.66E-49 | -1.69E-46 |
| MS.gene05938 | MS.gene40090 | 0.800041 | 1.74E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40090.t1 | MTR_4g092900 | 95.732 | 164 | 7 | 0 | 1 | 164 | 1 | 164 | 5.37e-111 | 325 |
| MS.gene40090.t1 | MTR_4g092900 | 95.732 | 164 | 7 | 0 | 1 | 164 | 1 | 164 | 1.11e-110 | 325 |
| MS.gene40090.t1 | MTR_4g092880 | 74.854 | 171 | 33 | 3 | 1 | 164 | 1 | 168 | 3.22e-83 | 250 |
| MS.gene40090.t1 | MTR_4g092880 | 74.854 | 171 | 33 | 3 | 1 | 164 | 1 | 168 | 1.09e-81 | 250 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40090.t1 | AT5G65630 | 34.896 | 192 | 94 | 6 | 1 | 164 | 1 | 189 | 1.24e-16 | 76.6 |
| MS.gene40090.t1 | AT5G10550 | 33.673 | 196 | 96 | 6 | 1 | 164 | 1 | 194 | 4.42e-15 | 72.0 |
Find 40 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTGTGTTGCAGCCAATT+TGG | 0.258953 | 4.3:-21278014 | None:intergenic |
| TTGTTACTAAATTTAGGGTT+AGG | 0.292960 | 4.3:-21278071 | None:intergenic |
| TCAGGGAGCAAGAAGGTTTC+CGG | 0.305508 | 4.3:+21278326 | MS.gene40090:CDS |
| GAGTCTTAAGCTGACGAATT+TGG | 0.320746 | 4.3:-21278241 | None:intergenic |
| ACCACCGTTGTGGTTTAGCC+TGG | 0.356873 | 4.3:-21278288 | None:intergenic |
| ACAACCGTAACGAACCAAAT+TGG | 0.373779 | 4.3:+21278000 | MS.gene40090:CDS |
| AAGAAATCAAAGTCAGAGAT+TGG | 0.382024 | 4.3:+21278383 | MS.gene40090:CDS |
| CTTGCTCCCTGATTTCTTAG+TGG | 0.395685 | 4.3:-21278315 | None:intergenic |
| TTGCTCCCTGATTTCTTAGT+GGG | 0.400065 | 4.3:-21278314 | None:intergenic |
| GGTGGTCCCACTAAGAAATC+AGG | 0.406385 | 4.3:+21278308 | MS.gene40090:CDS |
| CTTAAGACTCGAATTGAATC+AGG | 0.417831 | 4.3:+21278254 | MS.gene40090:CDS |
| AGGAAAAGGTCGTTTGTTGC+CGG | 0.425299 | 4.3:-21278345 | None:intergenic |
| CTAAATTTAGGGTTAGGGTT+AGG | 0.433996 | 4.3:-21278065 | None:intergenic |
| AGAAATCAAAGTCAGAGATT+GGG | 0.434900 | 4.3:+21278384 | MS.gene40090:CDS |
| TGTTACTAAATTTAGGGTTA+GGG | 0.446081 | 4.3:-21278070 | None:intergenic |
| AGGCGGTGGAGCTGAATTCA+TGG | 0.450284 | 4.3:+21278034 | MS.gene40090:CDS |
| AATTCCTTCACCGCAGGAAA+AGG | 0.459833 | 4.3:-21278359 | None:intergenic |
| GCAGCCAATTTGGTTCGTTA+CGG | 0.464960 | 4.3:-21278004 | None:intergenic |
| AGAGATTGGGGATGCGATGA+AGG | 0.491863 | 4.3:+21278397 | MS.gene40090:CDS |
| GTTGGATCGTCGATTGATTG+AGG | 0.506931 | 4.3:-21278126 | None:intergenic |
| TTCTTCAATTCCTTCACCGC+AGG | 0.508468 | 4.3:-21278365 | None:intergenic |
| ATTGAGGAAGCGTCATCTGA+TGG | 0.524535 | 4.3:-21278110 | None:intergenic |
| TGGCTGCAACACAGAGGCGG+TGG | 0.525481 | 4.3:+21278020 | MS.gene40090:CDS |
| CCACCGTTGTGGTTTAGCCT+GGG | 0.530502 | 4.3:-21278287 | None:intergenic |
| TTGAGGAAGCGTCATCTGAT+GGG | 0.538515 | 4.3:-21278109 | None:intergenic |
| GTATTGTGAATGATTATCGT+TGG | 0.542418 | 4.3:-21278144 | None:intergenic |
| GAAATCAAAGTCAGAGATTG+GGG | 0.550032 | 4.3:+21278385 | MS.gene40090:CDS |
| GGCGGTGGAGCTGAATTCAT+GGG | 0.566531 | 4.3:+21278035 | MS.gene40090:CDS |
| CATCGAATCGTACACAAAGA+CGG | 0.584140 | 4.3:+21278178 | MS.gene40090:CDS |
| TAGTGGGACCACCACCGTTG+TGG | 0.584586 | 4.3:-21278298 | None:intergenic |
| AGGCTAAACCACAACGGTGG+TGG | 0.594359 | 4.3:+21278290 | MS.gene40090:CDS |
| GTGGTCCCACTAAGAAATCA+GGG | 0.612863 | 4.3:+21278309 | MS.gene40090:CDS |
| TGAGGAAGCGTCATCTGATG+GGG | 0.626247 | 4.3:-21278108 | None:intergenic |
| TAAGAAATCAGGGAGCAAGA+AGG | 0.642181 | 4.3:+21278319 | MS.gene40090:CDS |
| GGGGATGCGATGAAGGCTTG+TGG | 0.668675 | 4.3:+21278404 | MS.gene40090:CDS |
| AATTGGCTGCAACACAGAGG+CGG | 0.689298 | 4.3:+21278017 | MS.gene40090:CDS |
| AATCAGGTGAGTTTAAACCC+AGG | 0.697049 | 4.3:+21278270 | MS.gene40090:CDS |
| CCCAGGCTAAACCACAACGG+TGG | 0.730798 | 4.3:+21278287 | MS.gene40090:CDS |
| CCAAATTGGCTGCAACACAG+AGG | 0.734243 | 4.3:+21278014 | MS.gene40090:CDS |
| AAACCCAGGCTAAACCACAA+CGG | 0.749218 | 4.3:+21278284 | MS.gene40090:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTTTTGTTACTAAATTTA+GGG | - | chr4.3:21278079-21278098 | None:intergenic | 15.0% |
| !!! | CTCTTTTTGTTACTAAATTT+AGG | - | chr4.3:21278080-21278099 | None:intergenic | 20.0% |
| !! | TGTTACTAAATTTAGGGTTA+GGG | - | chr4.3:21278073-21278092 | None:intergenic | 25.0% |
| !! | TTGTTACTAAATTTAGGGTT+AGG | - | chr4.3:21278074-21278093 | None:intergenic | 25.0% |
| AAGAAATCAAAGTCAGAGAT+TGG | + | chr4.3:21278383-21278402 | MS.gene40090:CDS | 30.0% | |
| AAGCTCATGAAAAACAAAAG+TGG | + | chr4.3:21278440-21278459 | MS.gene40090:CDS | 30.0% | |
| AGAAATCAAAGTCAGAGATT+GGG | + | chr4.3:21278384-21278403 | MS.gene40090:CDS | 30.0% | |
| GTATTGTGAATGATTATCGT+TGG | - | chr4.3:21278147-21278166 | None:intergenic | 30.0% | |
| TCATGAAAAACAAAAGTGGT+TGG | + | chr4.3:21278444-21278463 | MS.gene40090:CDS | 30.0% | |
| CTTAAGACTCGAATTGAATC+AGG | + | chr4.3:21278254-21278273 | MS.gene40090:CDS | 35.0% | |
| GAAATCAAAGTCAGAGATTG+GGG | + | chr4.3:21278385-21278404 | MS.gene40090:CDS | 35.0% | |
| ! | CTAAATTTAGGGTTAGGGTT+AGG | - | chr4.3:21278068-21278087 | None:intergenic | 35.0% |
| AATCAGGTGAGTTTAAACCC+AGG | + | chr4.3:21278270-21278289 | MS.gene40090:CDS | 40.0% | |
| ACAACCGTAACGAACCAAAT+TGG | + | chr4.3:21278000-21278019 | MS.gene40090:CDS | 40.0% | |
| CATCGAATCGTACACAAAGA+CGG | + | chr4.3:21278178-21278197 | MS.gene40090:CDS | 40.0% | |
| GAGTCTTAAGCTGACGAATT+TGG | - | chr4.3:21278244-21278263 | None:intergenic | 40.0% | |
| TAAGAAATCAGGGAGCAAGA+AGG | + | chr4.3:21278319-21278338 | MS.gene40090:CDS | 40.0% | |
| TTGCTCCCTGATTTCTTAGT+GGG | - | chr4.3:21278317-21278336 | None:intergenic | 40.0% | |
| AAACCCAGGCTAAACCACAA+CGG | + | chr4.3:21278284-21278303 | MS.gene40090:CDS | 45.0% | |
| AATTCCTTCACCGCAGGAAA+AGG | - | chr4.3:21278362-21278381 | None:intergenic | 45.0% | |
| CTTGCTCCCTGATTTCTTAG+TGG | - | chr4.3:21278318-21278337 | None:intergenic | 45.0% | |
| GTGGTCCCACTAAGAAATCA+GGG | + | chr4.3:21278309-21278328 | MS.gene40090:CDS | 45.0% | |
| TTCTTCAATTCCTTCACCGC+AGG | - | chr4.3:21278368-21278387 | None:intergenic | 45.0% | |
| ! | AGGAAAAGGTCGTTTGTTGC+CGG | - | chr4.3:21278348-21278367 | None:intergenic | 45.0% |
| ! | CAACAAACGACCTTTTCCTG+CGG | + | chr4.3:21278349-21278368 | MS.gene40090:CDS | 45.0% |
| ! | GCAGCCAATTTGGTTCGTTA+CGG | - | chr4.3:21278007-21278026 | None:intergenic | 45.0% |
| ! | GTTGGATCGTCGATTGATTG+AGG | - | chr4.3:21278129-21278148 | None:intergenic | 45.0% |
| !! | ATTGAGGAAGCGTCATCTGA+TGG | - | chr4.3:21278113-21278132 | None:intergenic | 45.0% |
| !! | TTGAGGAAGCGTCATCTGAT+GGG | - | chr4.3:21278112-21278131 | None:intergenic | 45.0% |
| AATTGGCTGCAACACAGAGG+CGG | + | chr4.3:21278017-21278036 | MS.gene40090:CDS | 50.0% | |
| AGAGATTGGGGATGCGATGA+AGG | + | chr4.3:21278397-21278416 | MS.gene40090:CDS | 50.0% | |
| CCAAATTGGCTGCAACACAG+AGG | + | chr4.3:21278014-21278033 | MS.gene40090:CDS | 50.0% | |
| CCTCTGTGTTGCAGCCAATT+TGG | - | chr4.3:21278017-21278036 | None:intergenic | 50.0% | |
| GGTGGTCCCACTAAGAAATC+AGG | + | chr4.3:21278308-21278327 | MS.gene40090:CDS | 50.0% | |
| TCAGGGAGCAAGAAGGTTTC+CGG | + | chr4.3:21278326-21278345 | MS.gene40090:CDS | 50.0% | |
| !! | TGAGGAAGCGTCATCTGATG+GGG | - | chr4.3:21278111-21278130 | None:intergenic | 50.0% |
| ACCACCGTTGTGGTTTAGCC+TGG | - | chr4.3:21278291-21278310 | None:intergenic | 55.0% | |
| AGGCGGTGGAGCTGAATTCA+TGG | + | chr4.3:21278034-21278053 | MS.gene40090:CDS | 55.0% | |
| AGGCTAAACCACAACGGTGG+TGG | + | chr4.3:21278290-21278309 | MS.gene40090:CDS | 55.0% | |
| CCACCGTTGTGGTTTAGCCT+GGG | - | chr4.3:21278290-21278309 | None:intergenic | 55.0% | |
| GGCGGTGGAGCTGAATTCAT+GGG | + | chr4.3:21278035-21278054 | MS.gene40090:CDS | 55.0% | |
| ! | ACGACCTTTTCCTGCGGTGA+AGG | + | chr4.3:21278355-21278374 | MS.gene40090:CDS | 55.0% |
| CCCAGGCTAAACCACAACGG+TGG | + | chr4.3:21278287-21278306 | MS.gene40090:CDS | 60.0% | |
| GGGGATGCGATGAAGGCTTG+TGG | + | chr4.3:21278404-21278423 | MS.gene40090:CDS | 60.0% | |
| TAGTGGGACCACCACCGTTG+TGG | - | chr4.3:21278301-21278320 | None:intergenic | 60.0% | |
| TGGCTGCAACACAGAGGCGG+TGG | + | chr4.3:21278020-21278039 | MS.gene40090:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 21277981 | 21278475 | 21277981 | ID=MS.gene40090 |
| chr4.3 | mRNA | 21277981 | 21278475 | 21277981 | ID=MS.gene40090.t1;Parent=MS.gene40090 |
| chr4.3 | exon | 21277981 | 21278475 | 21277981 | ID=MS.gene40090.t1.exon1;Parent=MS.gene40090.t1 |
| chr4.3 | CDS | 21277981 | 21278475 | 21277981 | ID=cds.MS.gene40090.t1;Parent=MS.gene40090.t1 |
| Gene Sequence |
| Protein sequence |
