Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40274.t1 | AES73887.1 | 96.6 | 147 | 5 | 0 | 1 | 147 | 1 | 147 | 1.90E-73 | 285 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40274.t1 | G7J3I0 | 96.6 | 147 | 5 | 0 | 1 | 147 | 1 | 147 | 1.4e-73 | 285.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057754 | MS.gene40274 | 0.81385 | 2.08E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40274.t1 | MTR_3g109860 | 96.599 | 147 | 5 | 0 | 1 | 147 | 1 | 147 | 1.73e-102 | 290 |
| MS.gene40274.t1 | MTR_8g021190 | 41.818 | 110 | 62 | 2 | 36 | 144 | 30 | 138 | 4.13e-25 | 94.4 |
| MS.gene40274.t1 | MTR_4g125030 | 44.681 | 94 | 51 | 1 | 36 | 128 | 22 | 115 | 1.92e-21 | 84.0 |
| MS.gene40274.t1 | MTR_5g006430 | 42.222 | 90 | 48 | 1 | 36 | 125 | 2 | 87 | 6.15e-21 | 82.0 |
| MS.gene40274.t1 | MTR_3g105340 | 36.111 | 108 | 68 | 1 | 36 | 143 | 11 | 117 | 2.41e-19 | 78.6 |
| MS.gene40274.t1 | MTR_4g125010 | 41.053 | 95 | 54 | 1 | 36 | 128 | 24 | 118 | 7.00e-19 | 78.2 |
| MS.gene40274.t1 | MTR_1g014930 | 35.185 | 108 | 66 | 2 | 24 | 128 | 4 | 110 | 8.28e-18 | 75.1 |
| MS.gene40274.t1 | MTR_8g013990 | 34.146 | 123 | 79 | 2 | 4 | 126 | 31 | 151 | 1.07e-17 | 75.9 |
| MS.gene40274.t1 | MTR_2g101140 | 34.146 | 123 | 78 | 3 | 4 | 126 | 32 | 151 | 1.33e-17 | 75.9 |
| MS.gene40274.t1 | MTR_1g103550 | 37.500 | 88 | 50 | 2 | 36 | 122 | 2 | 85 | 9.95e-16 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40274.t1 | AT2G25780 | 37.143 | 140 | 86 | 2 | 8 | 145 | 7 | 146 | 1.31e-23 | 90.5 |
| MS.gene40274.t1 | AT1G79770 | 39.496 | 119 | 67 | 2 | 8 | 126 | 27 | 140 | 2.75e-20 | 82.0 |
| MS.gene40274.t1 | AT1G72510 | 38.947 | 95 | 57 | 1 | 35 | 128 | 32 | 126 | 1.93e-19 | 79.7 |
| MS.gene40274.t1 | AT1G72510 | 38.947 | 95 | 57 | 1 | 35 | 128 | 32 | 126 | 1.93e-19 | 79.7 |
| MS.gene40274.t1 | AT4G14819 | 41.111 | 90 | 47 | 2 | 36 | 125 | 2 | 85 | 2.26e-19 | 78.2 |
| MS.gene40274.t1 | AT3G22540 | 37.778 | 90 | 52 | 2 | 36 | 125 | 2 | 87 | 2.45e-17 | 72.8 |
| MS.gene40274.t1 | AT5G20670 | 40.278 | 72 | 43 | 0 | 34 | 105 | 20 | 91 | 2.03e-15 | 69.3 |
| MS.gene40274.t1 | AT2G09970 | 35.354 | 99 | 56 | 3 | 35 | 128 | 32 | 127 | 5.42e-14 | 65.9 |
| MS.gene40274.t1 | AT5G25840 | 30.612 | 147 | 83 | 3 | 8 | 139 | 4 | 146 | 1.14e-13 | 65.1 |
| MS.gene40274.t1 | AT1G54095 | 37.895 | 95 | 54 | 2 | 36 | 127 | 15 | 107 | 2.19e-13 | 63.9 |
Find 29 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAAAGTTAACGAGAGTTT+AGG | 0.311962 | 3.3:+91025302 | None:intergenic |
| ACAAAGTTAACGAGAGTTTA+GGG | 0.372573 | 3.3:+91025303 | None:intergenic |
| CAAAAGGAATTGTAAGGGAT+TGG | 0.373720 | 3.3:-91025249 | MS.gene40274:CDS |
| AAGAAGATTATTGTGGAAAG+TGG | 0.375021 | 3.3:-91025454 | MS.gene40274:CDS |
| TGATTGTGGCATTATATTCC+TGG | 0.387287 | 3.3:+91025333 | None:intergenic |
| TTTAGACAAAAGGAATTGTA+AGG | 0.387538 | 3.3:-91025255 | MS.gene40274:CDS |
| TCTCGTTAACTTTGTCTATG+AGG | 0.419678 | 3.3:-91025295 | MS.gene40274:CDS |
| CTAAGAGAAGTTTAGACAAA+AGG | 0.433359 | 3.3:-91025265 | MS.gene40274:CDS |
| AAAAGGAATTGTAAGGGATT+GGG | 0.455861 | 3.3:-91025248 | MS.gene40274:CDS |
| AAGGCTACGAAAGGCGGTCT+CGG | 0.484076 | 3.3:-91025615 | MS.gene40274:CDS |
| GAAGCTGTTAAAGATAAAGT+AGG | 0.501109 | 3.3:-91025413 | MS.gene40274:CDS |
| CTCGTTAACTTTGTCTATGA+GGG | 0.501305 | 3.3:-91025294 | MS.gene40274:CDS |
| CTTCTTTCATCCCACAACAT+TGG | 0.506214 | 3.3:+91025507 | None:intergenic |
| AATTAACAAATACTATAACT+TGG | 0.525508 | 3.3:-91025579 | MS.gene40274:CDS |
| GTCTCAGATGGATTTCAGCC+AGG | 0.530828 | 3.3:-91025351 | MS.gene40274:CDS |
| AGGAGTGTAAGTTATCCATA+AGG | 0.532438 | 3.3:-91025209 | None:intergenic |
| TTTAGGGTTAAGTCTGATTG+TGG | 0.540154 | 3.3:+91025319 | None:intergenic |
| AAGCTGAATGCCAATGTTGT+GGG | 0.549736 | 3.3:-91025517 | MS.gene40274:CDS |
| AAGCTGTTAAAGATAAAGTA+GGG | 0.550044 | 3.3:-91025412 | MS.gene40274:CDS |
| TGATGCTTTGAAGTCTCAGA+TGG | 0.552591 | 3.3:-91025363 | MS.gene40274:CDS |
| GAAGTTGAAGAAGATTATTG+TGG | 0.554061 | 3.3:-91025461 | MS.gene40274:CDS |
| TTAGACAAAAGGAATTGTAA+GGG | 0.554428 | 3.3:-91025254 | MS.gene40274:CDS |
| TGGGAATAACTAAGCTCACT+AGG | 0.561524 | 3.3:-91025229 | MS.gene40274:CDS |
| TATTGTGGAAAGTGGGTGTG+TGG | 0.596235 | 3.3:-91025446 | MS.gene40274:CDS |
| AGAAGATTATTGTGGAAAGT+GGG | 0.599804 | 3.3:-91025453 | MS.gene40274:CDS |
| GAGTCAGCAAAGGCTACGAA+AGG | 0.603985 | 3.3:-91025624 | MS.gene40274:CDS |
| GAAGCTGAATGCCAATGTTG+TGG | 0.606985 | 3.3:-91025518 | MS.gene40274:CDS |
| TCAGCAAAGGCTACGAAAGG+CGG | 0.643815 | 3.3:-91025621 | MS.gene40274:CDS |
| TATAGGAGGAGAGTCAGCAA+AGG | 0.654980 | 3.3:-91025634 | MS.gene40274:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAACAAATACTATAACT+TGG | - | chr3.3:91025378-91025397 | MS.gene40274:CDS | 15.0% |
| !! | ATGAATAAATTAGAGATTAA+TGG | + | chr3.3:91025231-91025250 | None:intergenic | 15.0% |
| !! | TGAATAAATTAGAGATTAAT+GGG | + | chr3.3:91025230-91025249 | None:intergenic | 15.0% |
| !!! | AATTTTCTGTTTCCATATAT+AGG | - | chr3.3:91025306-91025325 | MS.gene40274:CDS | 20.0% |
| ! | AAGCTGTTAAAGATAAAGTA+GGG | - | chr3.3:91025545-91025564 | MS.gene40274:CDS | 25.0% |
| ! | TTAGACAAAAGGAATTGTAA+GGG | - | chr3.3:91025703-91025722 | MS.gene40274:intron | 25.0% |
| ! | TTTAGACAAAAGGAATTGTA+AGG | - | chr3.3:91025702-91025721 | MS.gene40274:intron | 25.0% |
| AAAAGGAATTGTAAGGGATT+GGG | - | chr3.3:91025709-91025728 | MS.gene40274:intron | 30.0% | |
| AAGAAGATTATTGTGGAAAG+TGG | - | chr3.3:91025503-91025522 | MS.gene40274:CDS | 30.0% | |
| ACAAAGTTAACGAGAGTTTA+GGG | + | chr3.3:91025657-91025676 | None:intergenic | 30.0% | |
| AGAAGATTATTGTGGAAAGT+GGG | - | chr3.3:91025504-91025523 | MS.gene40274:CDS | 30.0% | |
| CTAAGAGAAGTTTAGACAAA+AGG | - | chr3.3:91025692-91025711 | MS.gene40274:intron | 30.0% | |
| GAAGCTGTTAAAGATAAAGT+AGG | - | chr3.3:91025544-91025563 | MS.gene40274:CDS | 30.0% | |
| GAAGTTGAAGAAGATTATTG+TGG | - | chr3.3:91025496-91025515 | MS.gene40274:CDS | 30.0% | |
| TTTCTGTTTCCATATATAGG+AGG | - | chr3.3:91025309-91025328 | MS.gene40274:CDS | 30.0% | |
| CAAAAGGAATTGTAAGGGAT+TGG | - | chr3.3:91025708-91025727 | MS.gene40274:intron | 35.0% | |
| CTCGTTAACTTTGTCTATGA+GGG | - | chr3.3:91025663-91025682 | MS.gene40274:intron | 35.0% | |
| GACAAAGTTAACGAGAGTTT+AGG | + | chr3.3:91025658-91025677 | None:intergenic | 35.0% | |
| TCTCGTTAACTTTGTCTATG+AGG | - | chr3.3:91025662-91025681 | MS.gene40274:intron | 35.0% | |
| TGATTGTGGCATTATATTCC+TGG | + | chr3.3:91025627-91025646 | None:intergenic | 35.0% | |
| ! | TTTAGGGTTAAGTCTGATTG+TGG | + | chr3.3:91025641-91025660 | None:intergenic | 35.0% |
| AAGCTGAATGCCAATGTTGT+GGG | - | chr3.3:91025440-91025459 | MS.gene40274:CDS | 40.0% | |
| CTTCTTTCATCCCACAACAT+TGG | + | chr3.3:91025453-91025472 | None:intergenic | 40.0% | |
| TGGGAATAACTAAGCTCACT+AGG | - | chr3.3:91025728-91025747 | MS.gene40274:intron | 40.0% | |
| !! | TGATGCTTTGAAGTCTCAGA+TGG | - | chr3.3:91025594-91025613 | MS.gene40274:CDS | 40.0% |
| GAAGCTGAATGCCAATGTTG+TGG | - | chr3.3:91025439-91025458 | MS.gene40274:CDS | 45.0% | |
| TATAGGAGGAGAGTCAGCAA+AGG | - | chr3.3:91025323-91025342 | MS.gene40274:CDS | 45.0% | |
| TATTGTGGAAAGTGGGTGTG+TGG | - | chr3.3:91025511-91025530 | MS.gene40274:CDS | 45.0% | |
| ! | GCTGACTCTCCTCCTATATA+TGG | + | chr3.3:91025321-91025340 | None:intergenic | 45.0% |
| GAGTCAGCAAAGGCTACGAA+AGG | - | chr3.3:91025333-91025352 | MS.gene40274:CDS | 50.0% | |
| TCAGCAAAGGCTACGAAAGG+CGG | - | chr3.3:91025336-91025355 | MS.gene40274:CDS | 50.0% | |
| ! | GTCTCAGATGGATTTCAGCC+AGG | - | chr3.3:91025606-91025625 | MS.gene40274:CDS | 50.0% |
| AAGGCTACGAAAGGCGGTCT+CGG | - | chr3.3:91025342-91025361 | MS.gene40274:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 91025211 | 91025768 | 91025211 | ID=MS.gene40274 |
| chr3.3 | mRNA | 91025211 | 91025768 | 91025211 | ID=MS.gene40274.t1;Parent=MS.gene40274 |
| chr3.3 | exon | 91025766 | 91025768 | 91025766 | ID=MS.gene40274.t1.exon1;Parent=MS.gene40274.t1 |
| chr3.3 | CDS | 91025766 | 91025768 | 91025766 | ID=cds.MS.gene40274.t1;Parent=MS.gene40274.t1 |
| chr3.3 | exon | 91025211 | 91025651 | 91025211 | ID=MS.gene40274.t1.exon2;Parent=MS.gene40274.t1 |
| chr3.3 | CDS | 91025211 | 91025651 | 91025211 | ID=cds.MS.gene40274.t1;Parent=MS.gene40274.t1 |
| Gene Sequence |
| Protein sequence |
