AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057754


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057754.t1 MTR_4g076940 98.413 63 1 0 1 63 41 103 6.40e-40 138
MS.gene057754.t1 MTR_4g076940 98.413 63 1 0 1 63 1 63 7.31e-40 137
MS.gene057754.t1 MTR_4g076900 96.774 31 1 0 33 63 9 39 1.05e-16 72.4
MS.gene057754.t1 MTR_4g076970 51.562 64 27 2 1 63 1 61 1.64e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057754.t1 AT1G51500 67.568 37 12 0 27 63 18 54 1.11e-13 63.5
MS.gene057754.t1 AT3G21090 64.865 37 13 0 27 63 19 55 2.69e-13 62.8
MS.gene057754.t1 AT1G51460 68.750 32 10 0 32 63 9 40 4.30e-11 56.2

Find 13 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGTTATGTTGCCAAATTT+TGG 0.179448 4.4:+36219666 MS.gene057754:CDS
GTTGGTCCTTTCCCAAAATT+TGG 0.179855 4.4:-36219678 None:intergenic
AGGTTGCTTAATGGACTAAA+TGG 0.247817 4.4:+36219705 MS.gene057754:CDS
CCAACTAAAAGGTTGCTTAA+TGG 0.302201 4.4:+36219696 MS.gene057754:CDS
TTGGGAAAGGACCAACTAAA+AGG 0.333669 4.4:+36219685 MS.gene057754:CDS
GCATATTGTGGTCTTAATAG+AGG 0.502246 4.4:+36219618 MS.gene057754:CDS
ATAGAGGAAGCTTCTTAGCA+TGG 0.554454 4.4:+36219634 MS.gene057754:CDS
TAGAGGAAGCTTCTTAGCAT+GGG 0.556492 4.4:+36219635 MS.gene057754:CDS
AGTGATGAGGAAGCATATTG+TGG 0.621490 4.4:+36219606 MS.gene057754:CDS
CAACAACAAAGGATGCAACA+TGG 0.635595 4.4:+36219578 MS.gene057754:CDS
CAACATGGACGAGAGTGATG+AGG 0.639956 4.4:+36219593 MS.gene057754:CDS
ATGGAGATTGAAGCAGCAAG+TGG 0.659585 4.4:+36219552 MS.gene057754:CDS
GCAAGTGGTAGCAACAACAA+AGG 0.683774 4.4:+36219567 MS.gene057754:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAGTTATGTTGCCAAATTT+TGG + chr4.4:36219666-36219685 MS.gene057754:CDS 30.0%
! GAGTTATGTTGCCAAATTTT+GGG + chr4.4:36219667-36219686 MS.gene057754:CDS 30.0%
CCAACTAAAAGGTTGCTTAA+TGG + chr4.4:36219696-36219715 MS.gene057754:CDS 35.0%
! CCATTAAGCAACCTTTTAGT+TGG - chr4.4:36219699-36219718 None:intergenic 35.0%
! GCATATTGTGGTCTTAATAG+AGG + chr4.4:36219618-36219637 MS.gene057754:CDS 35.0%
!! AGGTTGCTTAATGGACTAAA+TGG + chr4.4:36219705-36219724 MS.gene057754:CDS 35.0%
!! ATGTTGCCAAATTTTGGGAA+AGG + chr4.4:36219672-36219691 MS.gene057754:CDS 35.0%
ATAGAGGAAGCTTCTTAGCA+TGG + chr4.4:36219634-36219653 MS.gene057754:CDS 40.0%
CAACAACAAAGGATGCAACA+TGG + chr4.4:36219578-36219597 MS.gene057754:CDS 40.0%
GTTGGTCCTTTCCCAAAATT+TGG - chr4.4:36219681-36219700 None:intergenic 40.0%
TAGAGGAAGCTTCTTAGCAT+GGG + chr4.4:36219635-36219654 MS.gene057754:CDS 40.0%
TTGGGAAAGGACCAACTAAA+AGG + chr4.4:36219685-36219704 MS.gene057754:CDS 40.0%
! AGTGATGAGGAAGCATATTG+TGG + chr4.4:36219606-36219625 MS.gene057754:CDS 40.0%
ATGGAGATTGAAGCAGCAAG+TGG + chr4.4:36219552-36219571 MS.gene057754:CDS 45.0%
GCAAGTGGTAGCAACAACAA+AGG + chr4.4:36219567-36219586 MS.gene057754:CDS 45.0%
CAACATGGACGAGAGTGATG+AGG + chr4.4:36219593-36219612 MS.gene057754:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 36219552 36219741 36219552 ID=MS.gene057754
chr4.4 mRNA 36219552 36219741 36219552 ID=MS.gene057754.t1;Parent=MS.gene057754
chr4.4 exon 36219552 36219741 36219552 ID=MS.gene057754.t1.exon1;Parent=MS.gene057754.t1
chr4.4 CDS 36219552 36219741 36219552 ID=cds.MS.gene057754.t1;Parent=MS.gene057754.t1
Gene Sequence

>MS.gene057754

ATGGAGATTGAAGCAGCAAGTGGTAGCAACAACAAAGGATGCAACATGGACGAGAGTGATGAGGAAGCATATTGTGGTCTTAATAGAGGAAGCTTCTTAGCATGGGAAGATCTAAGAGTTATGTTGCCAAATTTTGGGAAAGGACCAACTAAAAGGTTGCTTAATGGACTAAATGGTTTTGCTGAGCCTG

Protein sequence

>MS.gene057754.t1

MEIEAASGSNNKGCNMDESDEEAYCGLNRGSFLAWEDLRVMLPNFGKGPTKRLLNGLNGFAEP