Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40500.t1 | XP_024641881.1 | 95.1 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 3.30E-148 | 534.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40500.t1 | Q9XIP8 | 79.4 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.4e-126 | 453.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40500.t1 | G7KKM5 | 95.1 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 2.4e-148 | 534.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058076 | MS.gene40500 | 0.996589 | 1.66E-229 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40500.t1 | MTR_5g007050 | 95.131 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 0.0 | 532 |
| MS.gene40500.t1 | MTR_6g046960 | 95.131 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 0.0 | 531 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40500.t1 | AT1G64980 | 78.277 | 267 | 55 | 2 | 3 | 267 | 12 | 277 | 3.54e-163 | 453 |
| MS.gene40500.t1 | AT1G64980 | 79.401 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.59e-162 | 451 |
| MS.gene40500.t1 | AT1G64980 | 79.401 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.59e-162 | 451 |
Find 60 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAGATCTGATTGCTTAAT+TGG | 0.085095 | 5.3:+2013379 | None:intergenic |
| TCCATCTTTGTAGTCTCTTT+TGG | 0.193307 | 5.3:+2013151 | None:intergenic |
| ATTGAAGACCAATTCTTTCT+AGG | 0.195136 | 5.3:+2013106 | None:intergenic |
| TCTCTATTTAGCTGATATTA+AGG | 0.242104 | 5.3:-2013233 | MS.gene40500:CDS |
| ACGTGAAGACATTGCATTTC+AGG | 0.259904 | 5.3:-2013452 | MS.gene40500:CDS |
| ATTTCCTTGAAGGACATAAT+AGG | 0.275347 | 5.3:-2012949 | MS.gene40500:CDS |
| AAGATTCTTAACACCTAGTT+TGG | 0.296665 | 5.3:-2013296 | MS.gene40500:CDS |
| TGGCTTGAGGATGATGAAAT+TGG | 0.336305 | 5.3:-2012992 | MS.gene40500:CDS |
| GGAGTAAGAACCTTGTTCTT+AGG | 0.346332 | 5.3:+2013061 | None:intergenic |
| TTTAGCTGATATTAAGGAAT+TGG | 0.346940 | 5.3:-2013227 | MS.gene40500:CDS |
| CTTTCCTTCATAGGTTTCAA+TGG | 0.348106 | 5.3:-2013012 | MS.gene40500:CDS |
| GAGGGCCGTGGTTTGAGGCT+TGG | 0.352682 | 5.3:-2012874 | MS.gene40500:CDS |
| AGACTGGTGCTTTCCTTCAT+AGG | 0.356440 | 5.3:-2013021 | MS.gene40500:CDS |
| TCGGGGAGGGCCGTGGTTTG+AGG | 0.366626 | 5.3:-2012879 | MS.gene40500:CDS |
| ACGGCCCTCCCCGAGTATAA+TGG | 0.377630 | 5.3:+2012888 | None:intergenic |
| ATTGCGAATTTGCTGATCTA+TGG | 0.387264 | 5.3:-2012847 | MS.gene40500:CDS |
| TTTCCTTGAAGGACATAATA+GGG | 0.389168 | 5.3:-2012948 | MS.gene40500:CDS |
| TACTAGTTGGTAATGGTGAT+TGG | 0.390769 | 5.3:+2013548 | None:intergenic |
| TAGAAAGAATTGGTCTTCAA+TGG | 0.393256 | 5.3:-2013104 | MS.gene40500:CDS |
| CAGCCCTATTATGTCCTTCA+AGG | 0.399988 | 5.3:+2012945 | None:intergenic |
| TTAATTGGTATGATCTCAAC+AGG | 0.402966 | 5.3:+2013394 | None:intergenic |
| TCAAGGTTGGGCTATGTTTG+TGG | 0.418527 | 5.3:-2013266 | MS.gene40500:CDS |
| CCTAGTTTGGCAAATTATCA+AGG | 0.430049 | 5.3:-2013283 | MS.gene40500:CDS |
| CCTTGATAATTTGCCAAACT+AGG | 0.430154 | 5.3:+2013283 | None:intergenic |
| TTTGGCAAATTATCAAGGTT+GGG | 0.439482 | 5.3:-2013278 | MS.gene40500:CDS |
| AAACCCTTCAAGATCTTTGT+GGG | 0.439682 | 5.3:-2013484 | MS.gene40500:CDS |
| GTCTGTGAATTCACAGCATC+AGG | 0.443576 | 5.3:+2013040 | None:intergenic |
| CTGTGTATCCTAGAAAGAAT+TGG | 0.452193 | 5.3:-2013114 | MS.gene40500:CDS |
| TTTGTTTGGAATTTCCTTGA+AGG | 0.455406 | 5.3:-2012959 | MS.gene40500:CDS |
| CGAGTATAATGGATAGCCTT+AGG | 0.455563 | 5.3:+2012899 | None:intergenic |
| GTTTGGCAAATTATCAAGGT+TGG | 0.457948 | 5.3:-2013279 | MS.gene40500:CDS |
| GAAACCCTTCAAGATCTTTG+TGG | 0.462717 | 5.3:-2013485 | MS.gene40500:CDS |
| ATTCCATTACCATTACTAGT+TGG | 0.470434 | 5.3:+2013535 | None:intergenic |
| TCAAGGAAATTCCAAACAAA+AGG | 0.473734 | 5.3:+2012962 | None:intergenic |
| ATTATACTCGGGGAGGGCCG+TGG | 0.478358 | 5.3:-2012886 | MS.gene40500:CDS |
| CTAAGGCTATCCATTATACT+CGG | 0.478464 | 5.3:-2012898 | MS.gene40500:CDS |
| GTCGTCATTCAATCATGAAA+AGG | 0.493012 | 5.3:-2013426 | MS.gene40500:CDS |
| TTACCATTACTAGTTGGTAA+TGG | 0.495217 | 5.3:+2013541 | None:intergenic |
| TCCAAAAGAGACTACAAAGA+TGG | 0.508455 | 5.3:-2013152 | MS.gene40500:CDS |
| GGAATTGGTAGATTTGATTG+AGG | 0.511326 | 5.3:-2013212 | MS.gene40500:CDS |
| TTACCAACTAGTAATGGTAA+TGG | 0.521531 | 5.3:-2013538 | MS.gene40500:CDS |
| GGTCTTTATTGGCGTGAAAG+AGG | 0.522858 | 5.3:-2013349 | MS.gene40500:CDS |
| CAAGCCATTGAAACCTATGA+AGG | 0.539155 | 5.3:+2013008 | None:intergenic |
| ATAACCCACAAAGATCTTGA+AGG | 0.541294 | 5.3:+2013480 | None:intergenic |
| TCATAGGTTTCAATGGCTTG+AGG | 0.551220 | 5.3:-2013005 | MS.gene40500:CDS |
| TTCTTCCAAGCCTCAAACCA+CGG | 0.559243 | 5.3:+2012869 | None:intergenic |
| TAAGGCTATCCATTATACTC+GGG | 0.565710 | 5.3:-2012897 | MS.gene40500:CDS |
| TGATCTATGGCTGAATGAGA+TGG | 0.566426 | 5.3:-2012834 | MS.gene40500:CDS |
| TAACCCACAAAGATCTTGAA+GGG | 0.577120 | 5.3:+2013481 | None:intergenic |
| CTGTGGTCATCCTAAGAACA+AGG | 0.579621 | 5.3:-2013071 | MS.gene40500:CDS |
| TGATCCAACTACTTCACCTA+AGG | 0.585896 | 5.3:-2012915 | MS.gene40500:CDS |
| TCACCATTACCAACTAGTAA+TGG | 0.590252 | 5.3:-2013544 | MS.gene40500:CDS |
| ATAGCCTTAGGTGAAGTAGT+TGG | 0.594477 | 5.3:+2012911 | None:intergenic |
| GATGCTGTGAATTCACAGAC+TGG | 0.623826 | 5.3:-2013037 | MS.gene40500:CDS |
| AAAGAGACTACAAAGATGGA+TGG | 0.623848 | 5.3:-2013148 | MS.gene40500:CDS |
| GCTATCCATTATACTCGGGG+AGG | 0.652245 | 5.3:-2012893 | MS.gene40500:CDS |
| ACAGAGATGAGTTTGAGCAA+TGG | 0.659058 | 5.3:-2013577 | None:intergenic |
| CTATCCATTATACTCGGGGA+GGG | 0.668674 | 5.3:-2012892 | MS.gene40500:CDS |
| AAGGCTATCCATTATACTCG+GGG | 0.680190 | 5.3:-2012896 | MS.gene40500:CDS |
| TGAAATGCAATGTCTTCACG+TGG | 0.692922 | 5.3:+2013454 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATCAGATTTTTCTTTTT+TGG | + | chr5.3:2013571-2013590 | None:intergenic | 15.0% |
| ! | TCTCTATTTAGCTGATATTA+AGG | - | chr5.3:2013128-2013147 | MS.gene40500:CDS | 25.0% |
| ! | TTTAGCTGATATTAAGGAAT+TGG | - | chr5.3:2013134-2013153 | MS.gene40500:CDS | 25.0% |
| AAGATTCTTAACACCTAGTT+TGG | - | chr5.3:2013065-2013084 | MS.gene40500:CDS | 30.0% | |
| ATTCCATTACCATTACTAGT+TGG | + | chr5.3:2012829-2012848 | None:intergenic | 30.0% | |
| ATTGAAGACCAATTCTTTCT+AGG | + | chr5.3:2013258-2013277 | None:intergenic | 30.0% | |
| ATTTCCTTGAAGGACATAAT+AGG | - | chr5.3:2013412-2013431 | MS.gene40500:CDS | 30.0% | |
| TCAAGGAAATTCCAAACAAA+AGG | + | chr5.3:2013402-2013421 | None:intergenic | 30.0% | |
| TGAGAAAAAGTGGTCTTTAT+TGG | - | chr5.3:2013001-2013020 | MS.gene40500:CDS | 30.0% | |
| TTAATTGGTATGATCTCAAC+AGG | + | chr5.3:2012970-2012989 | None:intergenic | 30.0% | |
| TTACCAACTAGTAATGGTAA+TGG | - | chr5.3:2012823-2012842 | MS.gene40500:CDS | 30.0% | |
| TTTCCTTGAAGGACATAATA+GGG | - | chr5.3:2013413-2013432 | MS.gene40500:CDS | 30.0% | |
| TTTGGCAAATTATCAAGGTT+GGG | - | chr5.3:2013083-2013102 | MS.gene40500:CDS | 30.0% | |
| ! | TTTGTTTGGAATTTCCTTGA+AGG | - | chr5.3:2013402-2013421 | MS.gene40500:CDS | 30.0% |
| !! | TAGAAAGAATTGGTCTTCAA+TGG | - | chr5.3:2013257-2013276 | MS.gene40500:CDS | 30.0% |
| !! | TTACCATTACTAGTTGGTAA+TGG | + | chr5.3:2012823-2012842 | None:intergenic | 30.0% |
| !!! | TCAATGGTTTTGTATAACTG+TGG | - | chr5.3:2013273-2013292 | MS.gene40500:CDS | 30.0% |
| AAACCCTTCAAGATCTTTGT+GGG | - | chr5.3:2012877-2012896 | MS.gene40500:CDS | 35.0% | |
| AAAGAGACTACAAAGATGGA+TGG | - | chr5.3:2013213-2013232 | MS.gene40500:CDS | 35.0% | |
| ATAACCCACAAAGATCTTGA+AGG | + | chr5.3:2012884-2012903 | None:intergenic | 35.0% | |
| CAATCAGATCTGAGAAAAAG+TGG | - | chr5.3:2012991-2013010 | MS.gene40500:CDS | 35.0% | |
| CCTAGTTTGGCAAATTATCA+AGG | - | chr5.3:2013078-2013097 | MS.gene40500:CDS | 35.0% | |
| CTAAGGCTATCCATTATACT+CGG | - | chr5.3:2013463-2013482 | MS.gene40500:CDS | 35.0% | |
| CTCAGATCTGATTGCTTAAT+TGG | + | chr5.3:2012985-2013004 | None:intergenic | 35.0% | |
| CTGTGTATCCTAGAAAGAAT+TGG | - | chr5.3:2013247-2013266 | MS.gene40500:CDS | 35.0% | |
| CTTTCCTTCATAGGTTTCAA+TGG | - | chr5.3:2013349-2013368 | MS.gene40500:CDS | 35.0% | |
| GGAATTGGTAGATTTGATTG+AGG | - | chr5.3:2013149-2013168 | MS.gene40500:CDS | 35.0% | |
| GTCGTCATTCAATCATGAAA+AGG | - | chr5.3:2012935-2012954 | MS.gene40500:CDS | 35.0% | |
| GTTTGGCAAATTATCAAGGT+TGG | - | chr5.3:2013082-2013101 | MS.gene40500:CDS | 35.0% | |
| TAACCCACAAAGATCTTGAA+GGG | + | chr5.3:2012883-2012902 | None:intergenic | 35.0% | |
| TAAGGCTATCCATTATACTC+GGG | - | chr5.3:2013464-2013483 | MS.gene40500:CDS | 35.0% | |
| TCACCATTACCAACTAGTAA+TGG | - | chr5.3:2012817-2012836 | MS.gene40500:CDS | 35.0% | |
| TCCAAAAGAGACTACAAAGA+TGG | - | chr5.3:2013209-2013228 | MS.gene40500:CDS | 35.0% | |
| ! | CCTTGATAATTTGCCAAACT+AGG | + | chr5.3:2013081-2013100 | None:intergenic | 35.0% |
| ! | TCCATCTTTGTAGTCTCTTT+TGG | + | chr5.3:2013213-2013232 | None:intergenic | 35.0% |
| !! | ATTGCGAATTTGCTGATCTA+TGG | - | chr5.3:2013514-2013533 | MS.gene40500:CDS | 35.0% |
| !! | TACTAGTTGGTAATGGTGAT+TGG | + | chr5.3:2012816-2012835 | None:intergenic | 35.0% |
| !!! | TTGGTTCTGTACCTTTTGTT+TGG | - | chr5.3:2013388-2013407 | MS.gene40500:CDS | 35.0% |
| !!! | TTTCTTTTTTGGCTTGAGCA+AGG | + | chr5.3:2013560-2013579 | None:intergenic | 35.0% |
| AAGGCTATCCATTATACTCG+GGG | - | chr5.3:2013465-2013484 | MS.gene40500:CDS | 40.0% | |
| ACGTGAAGACATTGCATTTC+AGG | - | chr5.3:2012909-2012928 | MS.gene40500:CDS | 40.0% | |
| CAAGCCATTGAAACCTATGA+AGG | + | chr5.3:2013356-2013375 | None:intergenic | 40.0% | |
| GAAACCCTTCAAGATCTTTG+TGG | - | chr5.3:2012876-2012895 | MS.gene40500:CDS | 40.0% | |
| GGAGTAAGAACCTTGTTCTT+AGG | + | chr5.3:2013303-2013322 | None:intergenic | 40.0% | |
| TCATAGGTTTCAATGGCTTG+AGG | - | chr5.3:2013356-2013375 | MS.gene40500:CDS | 40.0% | |
| TGAAATGCAATGTCTTCACG+TGG | + | chr5.3:2012910-2012929 | None:intergenic | 40.0% | |
| TGATCCAACTACTTCACCTA+AGG | - | chr5.3:2013446-2013465 | MS.gene40500:CDS | 40.0% | |
| TGATCTATGGCTGAATGAGA+TGG | - | chr5.3:2013527-2013546 | MS.gene40500:CDS | 40.0% | |
| TGGCTTGAGGATGATGAAAT+TGG | - | chr5.3:2013369-2013388 | MS.gene40500:CDS | 40.0% | |
| ! | ATAGCCTTAGGTGAAGTAGT+TGG | + | chr5.3:2013453-2013472 | None:intergenic | 40.0% |
| !! | CGAGTATAATGGATAGCCTT+AGG | + | chr5.3:2013465-2013484 | None:intergenic | 40.0% |
| AGACTGGTGCTTTCCTTCAT+AGG | - | chr5.3:2013340-2013359 | MS.gene40500:CDS | 45.0% | |
| CAGCCCTATTATGTCCTTCA+AGG | + | chr5.3:2013419-2013438 | None:intergenic | 45.0% | |
| CTATCCATTATACTCGGGGA+GGG | - | chr5.3:2013469-2013488 | MS.gene40500:CDS | 45.0% | |
| CTGTGGTCATCCTAAGAACA+AGG | - | chr5.3:2013290-2013309 | MS.gene40500:CDS | 45.0% | |
| GATGCTGTGAATTCACAGAC+TGG | - | chr5.3:2013324-2013343 | MS.gene40500:CDS | 45.0% | |
| GGTCTTTATTGGCGTGAAAG+AGG | - | chr5.3:2013012-2013031 | MS.gene40500:CDS | 45.0% | |
| GTCTGTGAATTCACAGCATC+AGG | + | chr5.3:2013324-2013343 | None:intergenic | 45.0% | |
| TCAAGGTTGGGCTATGTTTG+TGG | - | chr5.3:2013095-2013114 | MS.gene40500:CDS | 45.0% | |
| TTCTTCCAAGCCTCAAACCA+CGG | + | chr5.3:2013495-2013514 | None:intergenic | 45.0% | |
| GCTATCCATTATACTCGGGG+AGG | - | chr5.3:2013468-2013487 | MS.gene40500:CDS | 50.0% | |
| ACGGCCCTCCCCGAGTATAA+TGG | + | chr5.3:2013476-2013495 | None:intergenic | 60.0% | |
| ATTATACTCGGGGAGGGCCG+TGG | - | chr5.3:2013475-2013494 | MS.gene40500:CDS | 60.0% | |
| ! | GAGGGCCGTGGTTTGAGGCT+TGG | - | chr5.3:2013487-2013506 | MS.gene40500:CDS | 65.0% |
| TCGGGGAGGGCCGTGGTTTG+AGG | - | chr5.3:2013482-2013501 | MS.gene40500:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 2012790 | 2013593 | 2012790 | ID=MS.gene40500 |
| chr5.3 | mRNA | 2012790 | 2013593 | 2012790 | ID=MS.gene40500.t1;Parent=MS.gene40500 |
| chr5.3 | exon | 2012790 | 2013593 | 2012790 | ID=MS.gene40500.t1.exon1;Parent=MS.gene40500.t1 |
| chr5.3 | CDS | 2012790 | 2013593 | 2012790 | ID=cds.MS.gene40500.t1;Parent=MS.gene40500.t1 |
| Gene Sequence |
| Protein sequence |
