Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058076.t1 | XP_024641881.1 | 95.1 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 3.30E-148 | 534.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058076.t1 | Q9XIP8 | 79.4 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.4e-126 | 453.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058076.t1 | G7KKM5 | 95.1 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 2.4e-148 | 534.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058076 | MS.gene062332 | -0.807391 | 5.17E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene070857 | 0.823228 | 1.55E-53 | -1.69E-46 |
| MS.gene058076 | MS.gene072376 | 0.806951 | 6.41E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene08658 | 0.807341 | 5.30E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene09072 | 0.803534 | 3.33E-49 | -1.69E-46 |
| MS.gene058076 | MS.gene21094 | -0.815084 | 1.11E-51 | -1.69E-46 |
| MS.gene058076 | MS.gene21238 | -0.809443 | 1.89E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene21789 | 0.899976 | 1.16E-77 | -1.69E-46 |
| MS.gene058076 | MS.gene33046 | 0.814055 | 1.87E-51 | -1.69E-46 |
| MS.gene058076 | MS.gene38166 | 0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene058076 | MS.gene40500 | 0.996589 | 1.66E-229 | -1.69E-46 |
| MS.gene058076 | MS.gene40650 | -0.818516 | 1.88E-52 | -1.69E-46 |
| MS.gene058076 | MS.gene44351 | 0.807211 | 5.65E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene44439 | -0.804566 | 2.03E-49 | -1.69E-46 |
| MS.gene058076 | MS.gene45607 | -0.807285 | 5.45E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene58132 | 0.808137 | 3.59E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene59101 | -0.804638 | 1.96E-49 | -1.69E-46 |
| MS.gene058076 | MS.gene60176 | -0.802871 | 4.57E-49 | -1.69E-46 |
| MS.gene058076 | MS.gene60177 | -0.805661 | 1.20E-49 | -1.69E-46 |
| MS.gene058076 | MS.gene61144 | -0.806141 | 9.50E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene62955 | -0.809801 | 1.58E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene65233 | 0.837709 | 4.43E-57 | -1.69E-46 |
| MS.gene058076 | MS.gene72416 | -0.814443 | 1.54E-51 | -1.69E-46 |
| MS.gene058076 | MS.gene73805 | 0.805687 | 1.18E-49 | -1.69E-46 |
| MS.gene058076 | MS.gene74638 | 0.816672 | 4.90E-52 | -1.69E-46 |
| MS.gene058076 | MS.gene78428 | 0.808751 | 2.65E-50 | -1.69E-46 |
| MS.gene058076 | MS.gene82778 | 0.818193 | 2.23E-52 | -1.69E-46 |
| MS.gene058076 | MS.gene86517 | 0.814194 | 1.74E-51 | -1.69E-46 |
| MS.gene058076 | MS.gene95302 | -0.829086 | 6.27E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058076.t1 | MTR_5g007050 | 95.131 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 0.0 | 532 |
| MS.gene058076.t1 | MTR_6g046960 | 95.131 | 267 | 7 | 1 | 1 | 267 | 1 | 261 | 0.0 | 531 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058076.t1 | AT1G64980 | 78.277 | 267 | 55 | 2 | 3 | 267 | 12 | 277 | 3.54e-163 | 453 |
| MS.gene058076.t1 | AT1G64980 | 79.401 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.59e-162 | 451 |
| MS.gene058076.t1 | AT1G64980 | 79.401 | 267 | 45 | 3 | 1 | 267 | 1 | 257 | 1.59e-162 | 451 |
Find 60 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAGATCTGATTGCTTAAT+TGG | 0.085095 | 5.1:+1695020 | None:intergenic |
| TCCATCTTTGTAGTCTCTTT+TGG | 0.193307 | 5.1:+1694792 | None:intergenic |
| ATTGAAGACCAATTCTTTCT+AGG | 0.195136 | 5.1:+1694747 | None:intergenic |
| TCTCTATTTAGCTGATATTA+AGG | 0.242104 | 5.1:-1694874 | MS.gene058076:CDS |
| ACGTGAAGACATTGCATTTC+AGG | 0.259904 | 5.1:-1695093 | MS.gene058076:CDS |
| ATTTCCTTGAAGGACATAAT+AGG | 0.275347 | 5.1:-1694590 | MS.gene058076:CDS |
| AAGATTCTTAACACCTAGTT+TGG | 0.296665 | 5.1:-1694937 | MS.gene058076:CDS |
| TGGCTTGAGGATGATGAAAT+TGG | 0.336305 | 5.1:-1694633 | MS.gene058076:CDS |
| GGAGTAAGAACCTTGTTCTT+AGG | 0.346332 | 5.1:+1694702 | None:intergenic |
| TTTAGCTGATATTAAGGAAT+TGG | 0.346940 | 5.1:-1694868 | MS.gene058076:CDS |
| CTTTCCTTCATAGGTTTCAA+TGG | 0.348106 | 5.1:-1694653 | MS.gene058076:CDS |
| GAGGGCCGTGGTTTGAGGCT+TGG | 0.352682 | 5.1:-1694515 | MS.gene058076:CDS |
| AGACTGGTGCTTTCCTTCAT+AGG | 0.356440 | 5.1:-1694662 | MS.gene058076:CDS |
| TCGGGGAGGGCCGTGGTTTG+AGG | 0.366626 | 5.1:-1694520 | MS.gene058076:CDS |
| ACGGCCCTCCCCGAGTATAA+TGG | 0.377630 | 5.1:+1694529 | None:intergenic |
| ATTGCGAATTTGCTGATCTA+TGG | 0.387264 | 5.1:-1694488 | MS.gene058076:CDS |
| TTTCCTTGAAGGACATAATA+GGG | 0.389168 | 5.1:-1694589 | MS.gene058076:CDS |
| TACTAGTTGGTAATGGTGAT+TGG | 0.390769 | 5.1:+1695189 | None:intergenic |
| TAGAAAGAATTGGTCTTCAA+TGG | 0.393256 | 5.1:-1694745 | MS.gene058076:CDS |
| CAGCCCTATTATGTCCTTCA+AGG | 0.399988 | 5.1:+1694586 | None:intergenic |
| TTAATTGGTATGATCTCAAC+AGG | 0.402966 | 5.1:+1695035 | None:intergenic |
| TCAAGGTTGGGCTATGTTTG+TGG | 0.418527 | 5.1:-1694907 | MS.gene058076:CDS |
| CCTAGTTTGGCAAATTATCA+AGG | 0.430049 | 5.1:-1694924 | MS.gene058076:CDS |
| CCTTGATAATTTGCCAAACT+AGG | 0.430154 | 5.1:+1694924 | None:intergenic |
| TTTGGCAAATTATCAAGGTT+GGG | 0.439482 | 5.1:-1694919 | MS.gene058076:CDS |
| AAACCCTTCAAGATCTTTGT+GGG | 0.439682 | 5.1:-1695125 | MS.gene058076:CDS |
| GTCTGTGAATTCACAGCATC+AGG | 0.443576 | 5.1:+1694681 | None:intergenic |
| CTGTGTATCCTAGAAAGAAT+TGG | 0.452193 | 5.1:-1694755 | MS.gene058076:CDS |
| TTTGTTTGGAATTTCCTTGA+AGG | 0.455406 | 5.1:-1694600 | MS.gene058076:CDS |
| CGAGTATAATGGATAGCCTT+AGG | 0.455563 | 5.1:+1694540 | None:intergenic |
| GTTTGGCAAATTATCAAGGT+TGG | 0.457948 | 5.1:-1694920 | MS.gene058076:CDS |
| GAAACCCTTCAAGATCTTTG+TGG | 0.462717 | 5.1:-1695126 | MS.gene058076:CDS |
| ATTCCATTACCATTACTAGT+TGG | 0.470434 | 5.1:+1695176 | None:intergenic |
| TCAAGGAAATTCCAAACAAA+AGG | 0.473734 | 5.1:+1694603 | None:intergenic |
| ATTATACTCGGGGAGGGCCG+TGG | 0.478358 | 5.1:-1694527 | MS.gene058076:CDS |
| CTAAGGCTATCCATTATACT+CGG | 0.478464 | 5.1:-1694539 | MS.gene058076:CDS |
| GTCGTCATTCAATCATGAAA+AGG | 0.493012 | 5.1:-1695067 | MS.gene058076:CDS |
| TTACCATTACTAGTTGGTAA+TGG | 0.495217 | 5.1:+1695182 | None:intergenic |
| TCCAAAAGAGACTACAAAGA+TGG | 0.508455 | 5.1:-1694793 | MS.gene058076:CDS |
| GGAATTGGTAGATTTGATTG+AGG | 0.511326 | 5.1:-1694853 | MS.gene058076:CDS |
| TTACCAACTAGTAATGGTAA+TGG | 0.521531 | 5.1:-1695179 | MS.gene058076:CDS |
| GGTCTTTATTGGCGTGAAAG+AGG | 0.522858 | 5.1:-1694990 | MS.gene058076:CDS |
| CAAGCCATTGAAACCTATGA+AGG | 0.539155 | 5.1:+1694649 | None:intergenic |
| ATAACCCACAAAGATCTTGA+AGG | 0.541294 | 5.1:+1695121 | None:intergenic |
| TCATAGGTTTCAATGGCTTG+AGG | 0.551220 | 5.1:-1694646 | MS.gene058076:CDS |
| TTCTTCCAAGCCTCAAACCA+CGG | 0.559243 | 5.1:+1694510 | None:intergenic |
| TAAGGCTATCCATTATACTC+GGG | 0.565710 | 5.1:-1694538 | MS.gene058076:CDS |
| TGATCTATGGCTGAATGAGA+TGG | 0.566426 | 5.1:-1694475 | MS.gene058076:CDS |
| TAACCCACAAAGATCTTGAA+GGG | 0.577120 | 5.1:+1695122 | None:intergenic |
| CTGTGGTCATCCTAAGAACA+AGG | 0.579621 | 5.1:-1694712 | MS.gene058076:CDS |
| TGATCCAACTACTTCACCTA+AGG | 0.585896 | 5.1:-1694556 | MS.gene058076:CDS |
| TCACCATTACCAACTAGTAA+TGG | 0.590252 | 5.1:-1695185 | MS.gene058076:CDS |
| ATAGCCTTAGGTGAAGTAGT+TGG | 0.594477 | 5.1:+1694552 | None:intergenic |
| GATGCTGTGAATTCACAGAC+TGG | 0.623826 | 5.1:-1694678 | MS.gene058076:CDS |
| AAAGAGACTACAAAGATGGA+TGG | 0.623848 | 5.1:-1694789 | MS.gene058076:CDS |
| GCTATCCATTATACTCGGGG+AGG | 0.652245 | 5.1:-1694534 | MS.gene058076:CDS |
| ACAGAGATGAGTTTGAGCAA+TGG | 0.659058 | 5.1:-1695218 | None:intergenic |
| CTATCCATTATACTCGGGGA+GGG | 0.668674 | 5.1:-1694533 | MS.gene058076:CDS |
| AAGGCTATCCATTATACTCG+GGG | 0.680190 | 5.1:-1694537 | MS.gene058076:CDS |
| TGAAATGCAATGTCTTCACG+TGG | 0.692922 | 5.1:+1695095 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATCAGATTTTTCTTTTT+TGG | + | chr5.1:1695212-1695231 | None:intergenic | 15.0% |
| ! | TCTCTATTTAGCTGATATTA+AGG | - | chr5.1:1694769-1694788 | MS.gene058076:CDS | 25.0% |
| ! | TTTAGCTGATATTAAGGAAT+TGG | - | chr5.1:1694775-1694794 | MS.gene058076:CDS | 25.0% |
| AAGATTCTTAACACCTAGTT+TGG | - | chr5.1:1694706-1694725 | MS.gene058076:CDS | 30.0% | |
| ATTCCATTACCATTACTAGT+TGG | + | chr5.1:1694470-1694489 | None:intergenic | 30.0% | |
| ATTGAAGACCAATTCTTTCT+AGG | + | chr5.1:1694899-1694918 | None:intergenic | 30.0% | |
| ATTTCCTTGAAGGACATAAT+AGG | - | chr5.1:1695053-1695072 | MS.gene058076:CDS | 30.0% | |
| TCAAGGAAATTCCAAACAAA+AGG | + | chr5.1:1695043-1695062 | None:intergenic | 30.0% | |
| TGAGAAAAAGTGGTCTTTAT+TGG | - | chr5.1:1694642-1694661 | MS.gene058076:CDS | 30.0% | |
| TTAATTGGTATGATCTCAAC+AGG | + | chr5.1:1694611-1694630 | None:intergenic | 30.0% | |
| TTACCAACTAGTAATGGTAA+TGG | - | chr5.1:1694464-1694483 | MS.gene058076:CDS | 30.0% | |
| TTTCCTTGAAGGACATAATA+GGG | - | chr5.1:1695054-1695073 | MS.gene058076:CDS | 30.0% | |
| TTTGGCAAATTATCAAGGTT+GGG | - | chr5.1:1694724-1694743 | MS.gene058076:CDS | 30.0% | |
| ! | TTTGTTTGGAATTTCCTTGA+AGG | - | chr5.1:1695043-1695062 | MS.gene058076:CDS | 30.0% |
| !! | TAGAAAGAATTGGTCTTCAA+TGG | - | chr5.1:1694898-1694917 | MS.gene058076:CDS | 30.0% |
| !! | TTACCATTACTAGTTGGTAA+TGG | + | chr5.1:1694464-1694483 | None:intergenic | 30.0% |
| !!! | TCAATGGTTTTGTATAACTG+TGG | - | chr5.1:1694914-1694933 | MS.gene058076:CDS | 30.0% |
| AAACCCTTCAAGATCTTTGT+GGG | - | chr5.1:1694518-1694537 | MS.gene058076:CDS | 35.0% | |
| AAAGAGACTACAAAGATGGA+TGG | - | chr5.1:1694854-1694873 | MS.gene058076:CDS | 35.0% | |
| ATAACCCACAAAGATCTTGA+AGG | + | chr5.1:1694525-1694544 | None:intergenic | 35.0% | |
| CAATCAGATCTGAGAAAAAG+TGG | - | chr5.1:1694632-1694651 | MS.gene058076:CDS | 35.0% | |
| CCTAGTTTGGCAAATTATCA+AGG | - | chr5.1:1694719-1694738 | MS.gene058076:CDS | 35.0% | |
| CTAAGGCTATCCATTATACT+CGG | - | chr5.1:1695104-1695123 | MS.gene058076:CDS | 35.0% | |
| CTCAGATCTGATTGCTTAAT+TGG | + | chr5.1:1694626-1694645 | None:intergenic | 35.0% | |
| CTGTGTATCCTAGAAAGAAT+TGG | - | chr5.1:1694888-1694907 | MS.gene058076:CDS | 35.0% | |
| CTTTCCTTCATAGGTTTCAA+TGG | - | chr5.1:1694990-1695009 | MS.gene058076:CDS | 35.0% | |
| GGAATTGGTAGATTTGATTG+AGG | - | chr5.1:1694790-1694809 | MS.gene058076:CDS | 35.0% | |
| GTCGTCATTCAATCATGAAA+AGG | - | chr5.1:1694576-1694595 | MS.gene058076:CDS | 35.0% | |
| GTTTGGCAAATTATCAAGGT+TGG | - | chr5.1:1694723-1694742 | MS.gene058076:CDS | 35.0% | |
| TAACCCACAAAGATCTTGAA+GGG | + | chr5.1:1694524-1694543 | None:intergenic | 35.0% | |
| TAAGGCTATCCATTATACTC+GGG | - | chr5.1:1695105-1695124 | MS.gene058076:CDS | 35.0% | |
| TCACCATTACCAACTAGTAA+TGG | - | chr5.1:1694458-1694477 | MS.gene058076:CDS | 35.0% | |
| TCCAAAAGAGACTACAAAGA+TGG | - | chr5.1:1694850-1694869 | MS.gene058076:CDS | 35.0% | |
| ! | CCTTGATAATTTGCCAAACT+AGG | + | chr5.1:1694722-1694741 | None:intergenic | 35.0% |
| ! | TCCATCTTTGTAGTCTCTTT+TGG | + | chr5.1:1694854-1694873 | None:intergenic | 35.0% |
| !! | ATTGCGAATTTGCTGATCTA+TGG | - | chr5.1:1695155-1695174 | MS.gene058076:CDS | 35.0% |
| !! | TACTAGTTGGTAATGGTGAT+TGG | + | chr5.1:1694457-1694476 | None:intergenic | 35.0% |
| !!! | TTGGTTCTGTACCTTTTGTT+TGG | - | chr5.1:1695029-1695048 | MS.gene058076:CDS | 35.0% |
| !!! | TTTCTTTTTTGGCTTGAGCA+AGG | + | chr5.1:1695201-1695220 | None:intergenic | 35.0% |
| AAGGCTATCCATTATACTCG+GGG | - | chr5.1:1695106-1695125 | MS.gene058076:CDS | 40.0% | |
| ACGTGAAGACATTGCATTTC+AGG | - | chr5.1:1694550-1694569 | MS.gene058076:CDS | 40.0% | |
| CAAGCCATTGAAACCTATGA+AGG | + | chr5.1:1694997-1695016 | None:intergenic | 40.0% | |
| GAAACCCTTCAAGATCTTTG+TGG | - | chr5.1:1694517-1694536 | MS.gene058076:CDS | 40.0% | |
| GGAGTAAGAACCTTGTTCTT+AGG | + | chr5.1:1694944-1694963 | None:intergenic | 40.0% | |
| TCATAGGTTTCAATGGCTTG+AGG | - | chr5.1:1694997-1695016 | MS.gene058076:CDS | 40.0% | |
| TGAAATGCAATGTCTTCACG+TGG | + | chr5.1:1694551-1694570 | None:intergenic | 40.0% | |
| TGATCCAACTACTTCACCTA+AGG | - | chr5.1:1695087-1695106 | MS.gene058076:CDS | 40.0% | |
| TGATCTATGGCTGAATGAGA+TGG | - | chr5.1:1695168-1695187 | MS.gene058076:CDS | 40.0% | |
| TGGCTTGAGGATGATGAAAT+TGG | - | chr5.1:1695010-1695029 | MS.gene058076:CDS | 40.0% | |
| ! | ATAGCCTTAGGTGAAGTAGT+TGG | + | chr5.1:1695094-1695113 | None:intergenic | 40.0% |
| !! | CGAGTATAATGGATAGCCTT+AGG | + | chr5.1:1695106-1695125 | None:intergenic | 40.0% |
| AGACTGGTGCTTTCCTTCAT+AGG | - | chr5.1:1694981-1695000 | MS.gene058076:CDS | 45.0% | |
| CAGCCCTATTATGTCCTTCA+AGG | + | chr5.1:1695060-1695079 | None:intergenic | 45.0% | |
| CTATCCATTATACTCGGGGA+GGG | - | chr5.1:1695110-1695129 | MS.gene058076:CDS | 45.0% | |
| CTGTGGTCATCCTAAGAACA+AGG | - | chr5.1:1694931-1694950 | MS.gene058076:CDS | 45.0% | |
| GATGCTGTGAATTCACAGAC+TGG | - | chr5.1:1694965-1694984 | MS.gene058076:CDS | 45.0% | |
| GGTCTTTATTGGCGTGAAAG+AGG | - | chr5.1:1694653-1694672 | MS.gene058076:CDS | 45.0% | |
| GTCTGTGAATTCACAGCATC+AGG | + | chr5.1:1694965-1694984 | None:intergenic | 45.0% | |
| TCAAGGTTGGGCTATGTTTG+TGG | - | chr5.1:1694736-1694755 | MS.gene058076:CDS | 45.0% | |
| TTCTTCCAAGCCTCAAACCA+CGG | + | chr5.1:1695136-1695155 | None:intergenic | 45.0% | |
| GCTATCCATTATACTCGGGG+AGG | - | chr5.1:1695109-1695128 | MS.gene058076:CDS | 50.0% | |
| ACGGCCCTCCCCGAGTATAA+TGG | + | chr5.1:1695117-1695136 | None:intergenic | 60.0% | |
| ATTATACTCGGGGAGGGCCG+TGG | - | chr5.1:1695116-1695135 | MS.gene058076:CDS | 60.0% | |
| ! | GAGGGCCGTGGTTTGAGGCT+TGG | - | chr5.1:1695128-1695147 | MS.gene058076:CDS | 65.0% |
| TCGGGGAGGGCCGTGGTTTG+AGG | - | chr5.1:1695123-1695142 | MS.gene058076:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 1694431 | 1695234 | 1694431 | ID=MS.gene058076 |
| chr5.1 | mRNA | 1694431 | 1695234 | 1694431 | ID=MS.gene058076.t1;Parent=MS.gene058076 |
| chr5.1 | exon | 1694431 | 1695234 | 1694431 | ID=MS.gene058076.t1.exon1;Parent=MS.gene058076.t1 |
| chr5.1 | CDS | 1694431 | 1695234 | 1694431 | ID=cds.MS.gene058076.t1;Parent=MS.gene058076.t1 |
| Gene Sequence |
| Protein sequence |
