Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40636.t1 | AES89058.1 | 90.7 | 75 | 6 | 1 | 1 | 75 | 1 | 74 | 2.40E-27 | 131 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40636.t1 | G7JF00 | 90.7 | 75 | 6 | 1 | 1 | 75 | 1 | 74 | 1.7e-27 | 131.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056623 | MS.gene40636 | 0.976909 | 9.79E-143 | -1.69E-46 |
| MS.gene059856 | MS.gene40636 | 0.819223 | 1.30E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40636.t1 | MTR_4g068560 | 90.667 | 75 | 6 | 1 | 1 | 75 | 1 | 74 | 2.48e-43 | 134 |
| MS.gene40636.t1 | MTR_4g068453 | 70.667 | 75 | 18 | 2 | 1 | 75 | 1 | 71 | 9.65e-30 | 100 |
| MS.gene40636.t1 | MTR_1g034290 | 52.000 | 75 | 31 | 3 | 1 | 74 | 1 | 71 | 9.93e-19 | 72.8 |
| MS.gene40636.t1 | MTR_1g034270 | 44.595 | 74 | 33 | 1 | 1 | 74 | 1 | 66 | 1.24e-16 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40636.t1 | AT2G15960 | 40.244 | 82 | 37 | 2 | 1 | 75 | 1 | 77 | 8.48e-14 | 60.5 |
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAACCGTGTGTTTGCTTA+TGG | 0.279387 | 4.4:-38856230 | MS.gene40636:CDS |
| CCATTGTAGAGGTATGGGTT+GGG | 0.390310 | 4.4:-38856408 | MS.gene40636:CDS |
| AGGCAACAGAGGTGTCTCTT+GGG | 0.412772 | 4.4:+38856275 | None:intergenic |
| TTATGGATCGATTTGTTCCT+TGG | 0.422668 | 4.4:-38856213 | MS.gene40636:CDS |
| TGGCTGCCATTGTAGAGGTA+TGG | 0.433651 | 4.4:-38856414 | MS.gene40636:CDS |
| GAGGCAACAGAGGTGTCTCT+TGG | 0.459401 | 4.4:+38856274 | None:intergenic |
| CATTGTAGAGGTATGGGTTG+GGG | 0.470237 | 4.4:-38856407 | MS.gene40636:CDS |
| GCCATTGTAGAGGTATGGGT+TGG | 0.477069 | 4.4:-38856409 | MS.gene40636:CDS |
| GGCAACAGAGGTGTCTCTTG+GGG | 0.546169 | 4.4:+38856276 | None:intergenic |
| GGCTGCCATTGTAGAGGTAT+GGG | 0.559523 | 4.4:-38856413 | MS.gene40636:CDS |
| AACAAAGGCTTGCTGTTGCT+AGG | 0.594023 | 4.4:+38856355 | None:intergenic |
| TGAAGAGTCAGAGGCAACAG+AGG | 0.597712 | 4.4:+38856264 | None:intergenic |
| ATTAATGGCTGCCATTGTAG+AGG | 0.614505 | 4.4:-38856419 | None:intergenic |
| TGACATAGTTGAAGAGTCAG+AGG | 0.626699 | 4.4:+38856255 | None:intergenic |
| CCCAACCCATACCTCTACAA+TGG | 0.629283 | 4.4:+38856408 | None:intergenic |
| CAAAACAAGAAGAAGAACGT+TGG | 0.651156 | 4.4:-38856307 | MS.gene40636:CDS |
| TGACTCTTCAACTATGTCAG+AGG | 0.654740 | 4.4:-38856251 | MS.gene40636:CDS |
| GCAACAGAGGTGTCTCTTGG+GGG | 0.674229 | 4.4:+38856277 | None:intergenic |
| ATTAACATATACTATCACCA+AGG | 0.710327 | 4.4:+38856196 | None:intergenic |
| TCGATCCATAAGCAAACACA+CGG | 0.710548 | 4.4:+38856225 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGTTTTCAAAAGCCAAACA+AGG | - | chr4.4:38856288-38856307 | MS.gene40636:CDS | 30.0% |
| !!! | TGTTTGGCTTTTGAAAACAA+AGG | + | chr4.4:38856288-38856307 | None:intergenic | 30.0% |
| CAAAACAAGAAGAAGAACGT+TGG | - | chr4.4:38856318-38856337 | MS.gene40636:CDS | 35.0% | |
| !! | TTATGGATCGATTTGTTCCT+TGG | - | chr4.4:38856412-38856431 | MS.gene40636:CDS | 35.0% |
| TCGATCCATAAGCAAACACA+CGG | + | chr4.4:38856403-38856422 | None:intergenic | 40.0% | |
| TGACATAGTTGAAGAGTCAG+AGG | + | chr4.4:38856373-38856392 | None:intergenic | 40.0% | |
| TGACTCTTCAACTATGTCAG+AGG | - | chr4.4:38856374-38856393 | MS.gene40636:CDS | 40.0% | |
| ! | CATTGTAGAGGTATGGGTTG+GGG | - | chr4.4:38856218-38856237 | MS.gene40636:CDS | 45.0% |
| ! | CCATTGTAGAGGTATGGGTT+GGG | - | chr4.4:38856217-38856236 | MS.gene40636:CDS | 45.0% |
| !! | AACAAAGGCTTGCTGTTGCT+AGG | + | chr4.4:38856273-38856292 | None:intergenic | 45.0% |
| !! | GTTTTGATCTGCGCCTTGTT+TGG | + | chr4.4:38856304-38856323 | None:intergenic | 45.0% |
| CCCAACCCATACCTCTACAA+TGG | + | chr4.4:38856220-38856239 | None:intergenic | 50.0% | |
| GGCAACCGTGTGTTTGCTTA+TGG | - | chr4.4:38856395-38856414 | MS.gene40636:CDS | 50.0% | |
| TGAAGAGTCAGAGGCAACAG+AGG | + | chr4.4:38856364-38856383 | None:intergenic | 50.0% | |
| TGGCTGCCATTGTAGAGGTA+TGG | - | chr4.4:38856211-38856230 | MS.gene40636:CDS | 50.0% | |
| ! | GCCATTGTAGAGGTATGGGT+TGG | - | chr4.4:38856216-38856235 | MS.gene40636:CDS | 50.0% |
| ! | GGCTGCCATTGTAGAGGTAT+GGG | - | chr4.4:38856212-38856231 | MS.gene40636:CDS | 50.0% |
| !! | AGGCAACAGAGGTGTCTCTT+GGG | + | chr4.4:38856353-38856372 | None:intergenic | 50.0% |
| !! | GAGGCAACAGAGGTGTCTCT+TGG | + | chr4.4:38856354-38856373 | None:intergenic | 55.0% |
| !! | GCAACAGAGGTGTCTCTTGG+GGG | + | chr4.4:38856351-38856370 | None:intergenic | 55.0% |
| !! | GGCAACAGAGGTGTCTCTTG+GGG | + | chr4.4:38856352-38856371 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 38856210 | 38856437 | 38856210 | ID=MS.gene40636 |
| chr4.4 | mRNA | 38856210 | 38856437 | 38856210 | ID=MS.gene40636.t1;Parent=MS.gene40636 |
| chr4.4 | exon | 38856210 | 38856437 | 38856210 | ID=MS.gene40636.t1.exon1;Parent=MS.gene40636.t1 |
| chr4.4 | CDS | 38856210 | 38856437 | 38856210 | ID=cds.MS.gene40636.t1;Parent=MS.gene40636.t1 |
| Gene Sequence |
| Protein sequence |
