Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40638.t1 | XP_013456325.2 | 86.1 | 509 | 66 | 3 | 1 | 507 | 1 | 506 | 3.50E-191 | 677.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40638.t1 | O48716 | 49.2 | 453 | 199 | 9 | 21 | 472 | 6 | 428 | 1.3e-77 | 292.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40638.t1 | A0A396IEE4 | 86.1 | 509 | 66 | 3 | 1 | 507 | 1 | 506 | 2.5e-191 | 677.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056624 | MS.gene40638 | 0.892391 | 1.66E-74 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene027729 | MS.gene40638 | PPI |
| MS.gene056316 | MS.gene40638 | PPI |
| MS.gene32239 | MS.gene40638 | PPI |
| MS.gene86222 | MS.gene40638 | PPI |
| MS.gene44289 | MS.gene40638 | PPI |
| MS.gene39202 | MS.gene40638 | PPI |
| MS.gene007856 | MS.gene40638 | PPI |
| MS.gene46486 | MS.gene40638 | PPI |
| MS.gene006293 | MS.gene40638 | PPI |
| MS.gene49890 | MS.gene40638 | PPI |
| MS.gene38997 | MS.gene40638 | PPI |
| MS.gene34495 | MS.gene40638 | PPI |
| MS.gene29165 | MS.gene40638 | PPI |
| MS.gene021991 | MS.gene40638 | PPI |
| MS.gene034624 | MS.gene40638 | PPI |
| MS.gene49091 | MS.gene40638 | PPI |
| MS.gene41434 | MS.gene40638 | PPI |
| MS.gene032322 | MS.gene40638 | PPI |
| MS.gene44717 | MS.gene40638 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40638.t1 | MTR_4g068457 | 97.006 | 501 | 10 | 3 | 9 | 507 | 1 | 498 | 0.0 | 997 |
| MS.gene40638.t1 | MTR_1g021590 | 58.559 | 444 | 152 | 8 | 23 | 464 | 20 | 433 | 5.38e-176 | 503 |
| MS.gene40638.t1 | MTR_5g018520 | 50.858 | 466 | 184 | 8 | 2 | 467 | 4 | 424 | 3.20e-160 | 463 |
| MS.gene40638.t1 | MTR_3g078380 | 39.181 | 342 | 187 | 10 | 140 | 462 | 70 | 409 | 4.95e-74 | 240 |
| MS.gene40638.t1 | MTR_5g035980 | 38.441 | 372 | 180 | 8 | 138 | 462 | 74 | 443 | 1.94e-72 | 237 |
| MS.gene40638.t1 | MTR_5g035980 | 22.400 | 375 | 215 | 14 | 35 | 364 | 99 | 442 | 4.55e-11 | 65.5 |
| MS.gene40638.t1 | MTR_4g106480 | 37.901 | 343 | 191 | 6 | 137 | 462 | 59 | 396 | 2.14e-72 | 235 |
| MS.gene40638.t1 | MTR_3g110185 | 40.936 | 342 | 182 | 8 | 128 | 462 | 81 | 409 | 6.08e-72 | 244 |
| MS.gene40638.t1 | MTR_8g089650 | 37.500 | 360 | 204 | 8 | 115 | 463 | 80 | 429 | 4.82e-71 | 233 |
| MS.gene40638.t1 | MTR_2g060070 | 37.830 | 341 | 196 | 7 | 128 | 465 | 57 | 384 | 3.60e-70 | 230 |
| MS.gene40638.t1 | MTR_4g104410 | 39.640 | 333 | 186 | 8 | 137 | 462 | 39 | 363 | 1.42e-69 | 227 |
| MS.gene40638.t1 | MTR_2g101410 | 36.000 | 350 | 200 | 10 | 130 | 468 | 79 | 415 | 2.28e-62 | 210 |
| MS.gene40638.t1 | MTR_8g028700 | 25.723 | 311 | 195 | 13 | 120 | 407 | 214 | 511 | 2.61e-14 | 75.9 |
| MS.gene40638.t1 | MTR_8g032850 | 25.490 | 306 | 200 | 12 | 120 | 407 | 601 | 896 | 2.89e-13 | 73.2 |
| MS.gene40638.t1 | MTR_4g119620 | 25.877 | 228 | 133 | 9 | 244 | 460 | 119 | 321 | 2.55e-12 | 68.6 |
| MS.gene40638.t1 | MTR_8g104010 | 29.018 | 224 | 133 | 10 | 237 | 460 | 906 | 1103 | 5.47e-12 | 68.9 |
| MS.gene40638.t1 | MTR_8g104010 | 29.018 | 224 | 133 | 10 | 237 | 460 | 543 | 740 | 5.74e-12 | 68.9 |
| MS.gene40638.t1 | MTR_8g104010 | 29.018 | 224 | 133 | 10 | 237 | 460 | 626 | 823 | 6.56e-12 | 68.6 |
| MS.gene40638.t1 | MTR_6g465150 | 27.027 | 222 | 133 | 8 | 244 | 464 | 149 | 342 | 2.18e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40638.t1 | AT1G49450 | 53.347 | 493 | 172 | 10 | 9 | 466 | 1 | 470 | 2.23e-176 | 504 |
| MS.gene40638.t1 | AT3G18950 | 54.583 | 480 | 184 | 10 | 9 | 466 | 1 | 468 | 1.18e-175 | 503 |
| MS.gene40638.t1 | AT2G26490 | 57.978 | 445 | 156 | 9 | 21 | 464 | 6 | 420 | 4.12e-173 | 496 |
| MS.gene40638.t1 | AT4G34380 | 53.693 | 501 | 168 | 12 | 1 | 472 | 1 | 466 | 6.33e-168 | 484 |
| MS.gene40638.t1 | AT3G50390 | 56.784 | 398 | 160 | 7 | 78 | 469 | 40 | 431 | 9.75e-156 | 452 |
| MS.gene40638.t1 | AT1G47610 | 62.356 | 348 | 122 | 6 | 121 | 465 | 10 | 351 | 2.46e-145 | 421 |
| MS.gene40638.t1 | AT1G24130 | 42.061 | 359 | 178 | 8 | 128 | 463 | 62 | 413 | 5.96e-81 | 258 |
| MS.gene40638.t1 | AT5G50120 | 37.047 | 386 | 214 | 10 | 104 | 462 | 1 | 384 | 1.74e-77 | 248 |
| MS.gene40638.t1 | AT3G51930 | 39.489 | 352 | 191 | 9 | 128 | 465 | 54 | 397 | 3.08e-73 | 238 |
| MS.gene40638.t1 | AT1G24530 | 39.466 | 337 | 185 | 7 | 138 | 468 | 86 | 409 | 5.81e-73 | 238 |
| MS.gene40638.t1 | AT3G49660 | 26.522 | 230 | 131 | 9 | 242 | 460 | 110 | 312 | 2.05e-14 | 74.7 |
Find 128 sgRNAs with CRISPR-Local
Find 149 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATGGATTGGTTAACTTC+TGG | 0.213175 | 4.4:+38847109 | MS.gene40638:CDS |
| TGTAGAAAAGCACCAAATTT+TGG | 0.233924 | 4.4:-38846118 | None:intergenic |
| TTTATTGGTGCTATTATTAT+TGG | 0.250187 | 4.4:-38846086 | None:intergenic |
| CTGTCGCCGAAATATGTTCT+AGG | 0.279505 | 4.4:-38846217 | None:intergenic |
| TTGCTGACATTGATTGTTCC+TGG | 0.283768 | 4.4:-38847513 | None:intergenic |
| GAAGAAAACGAAGCATGTTT+TGG | 0.284274 | 4.4:+38847005 | MS.gene40638:CDS |
| GATAGTTTATACTGGTAGTT+TGG | 0.292066 | 4.4:+38847380 | MS.gene40638:CDS |
| TGATGGATTGGTTAACTTCT+GGG | 0.293708 | 4.4:+38847110 | MS.gene40638:CDS |
| ATCTACTCCTTAGCCGTTTC+CGG | 0.295728 | 4.4:+38846454 | MS.gene40638:CDS |
| GACAGCTATTTCCCGGAAAA+TGG | 0.300025 | 4.4:+38846397 | MS.gene40638:CDS |
| AAAATACATAGACCAAAATT+TGG | 0.308587 | 4.4:+38846106 | MS.gene40638:CDS |
| CGGACTTCTACGTAGTTCTT+TGG | 0.319774 | 4.4:-38846721 | None:intergenic |
| GGCGGTGGCTGTGGTGGTAT+TGG | 0.328170 | 4.4:-38846357 | None:intergenic |
| CATGGAGATAACATGTAATT+AGG | 0.330276 | 4.4:-38846262 | None:intergenic |
| AATTCTCCAATTGCACCTAT+TGG | 0.339564 | 4.4:+38847468 | MS.gene40638:CDS |
| TGAGAGGAGAAGAAGTTTCT+TGG | 0.355008 | 4.4:-38846288 | None:intergenic |
| GTCCTATGTCTCGCCGCGGC+AGG | 0.371031 | 4.4:+38847192 | MS.gene40638:CDS |
| GGGATAAAACTCTCAAAGTT+TGG | 0.381674 | 4.4:+38846836 | MS.gene40638:CDS |
| CACTGTTCCATCGGCTGAGC+CGG | 0.383779 | 4.4:-38846950 | None:intergenic |
| TGCTGTTTCGAGTCTGAGTT+TGG | 0.386320 | 4.4:+38846780 | MS.gene40638:CDS |
| TAATTCAATCCTTTGCCAAT+AGG | 0.387828 | 4.4:-38847483 | None:intergenic |
| TCTCAAAGTTTGGCGCGTTA+CGG | 0.407654 | 4.4:+38846846 | MS.gene40638:CDS |
| ACCACTGCTTGATTTAAAAC+CGG | 0.411447 | 4.4:-38846542 | None:intergenic |
| CCTTTGCCAATAGGTGCAAT+TGG | 0.413227 | 4.4:-38847474 | None:intergenic |
| CGTTACGGATTTCAAGTGTA+TGG | 0.422165 | 4.4:+38846861 | MS.gene40638:CDS |
| TCTAGAAATCATAAGCGTAT+TGG | 0.424001 | 4.4:+38846655 | MS.gene40638:CDS |
| CTGAACATGCGCCAGAGTTA+AGG | 0.425735 | 4.4:+38847430 | MS.gene40638:CDS |
| TCAGCAACAATAGTAATAGA+AGG | 0.431187 | 4.4:+38847529 | MS.gene40638:CDS |
| GGAGAGACATCGACGGAAAA+GGG | 0.432076 | 4.4:+38846983 | MS.gene40638:CDS |
| GCCTTGATCATCATCGTTGT+TGG | 0.434698 | 4.4:-38846167 | None:intergenic |
| TTGCGGTTCTTCTGATGGAT+TGG | 0.439337 | 4.4:+38847098 | MS.gene40638:CDS |
| GCGAGTCCTAGAACATATTT+CGG | 0.439823 | 4.4:+38846211 | MS.gene40638:CDS |
| TAGTTTGGATAAGTCTGTTA+AGG | 0.440081 | 4.4:+38847395 | MS.gene40638:CDS |
| TTATAACGACAGCTATTTCC+CGG | 0.443220 | 4.4:+38846390 | MS.gene40638:CDS |
| GTATAACATGTCACCGGAAA+CGG | 0.443548 | 4.4:-38846467 | None:intergenic |
| CCACACGCAAACATTCTTAT+CGG | 0.444510 | 4.4:-38847238 | None:intergenic |
| TTGACATAGTCTTTGAAAGT+TGG | 0.447390 | 4.4:-38846685 | None:intergenic |
| CATTGAATGACAAACACAAA+AGG | 0.448171 | 4.4:+38846037 | None:intergenic |
| TGCTGACATTGATTGTTCCT+GGG | 0.450251 | 4.4:-38847512 | None:intergenic |
| ATTTATTGCGGTTCTTCTGA+TGG | 0.451965 | 4.4:+38847093 | MS.gene40638:CDS |
| TTCCCGGAAAATGGCCTCAT+CGG | 0.452524 | 4.4:+38846406 | MS.gene40638:CDS |
| ACCGGTGAAGATGTTTCCTT+CGG | 0.460672 | 4.4:-38846587 | None:intergenic |
| AGGAGAGACATCGACGGAAA+AGG | 0.464125 | 4.4:+38846982 | MS.gene40638:CDS |
| CGGCCGAGGCGTTGCTGATA+CGG | 0.468033 | 4.4:-38846189 | None:intergenic |
| CAACGGTGGATAGTTTATAC+TGG | 0.469758 | 4.4:+38847372 | MS.gene40638:CDS |
| GAAGAAGTTTCTTGGTTCCA+TGG | 0.473762 | 4.4:-38846280 | None:intergenic |
| TGTGGAGGCGTGACGATAGA+GGG | 0.474106 | 4.4:+38847256 | MS.gene40638:CDS |
| TTCAACGGCAATGCATTTGA+CGG | 0.476271 | 4.4:-38847319 | None:intergenic |
| AATCGTTGATGAATGGTTAA+CGG | 0.476756 | 4.4:-38847070 | None:intergenic |
| TCCCGGAAAATGGCCTCATC+GGG | 0.480547 | 4.4:+38846407 | MS.gene40638:CDS |
| AATAAATAATCGTTGATGAA+TGG | 0.482054 | 4.4:-38847077 | None:intergenic |
| TCAACGGCAATGCATTTGAC+GGG | 0.487757 | 4.4:-38847318 | None:intergenic |
| TGGATAAGTCTGTTAAGGTC+TGG | 0.488240 | 4.4:+38847400 | MS.gene40638:CDS |
| TGTTGTCGTGGCGGTGGCTG+TGG | 0.492689 | 4.4:-38846366 | None:intergenic |
| AGGTTGATTGCTTCTGATGA+TGG | 0.495947 | 4.4:-38846331 | None:intergenic |
| TCCTATGTCTCGCCGCGGCA+GGG | 0.498073 | 4.4:+38847193 | MS.gene40638:CDS |
| GGTTGTTGTACTCAGGTTCG+TGG | 0.503075 | 4.4:+38846815 | MS.gene40638:CDS |
| ACCAGTGTGTCCCGTCAAAA+CGG | 0.506792 | 4.4:-38847295 | None:intergenic |
| TATAATTGTTGTCGTGGCGG+TGG | 0.508719 | 4.4:-38846372 | None:intergenic |
| GGCTGTGGTGGTATTGGAAG+AGG | 0.508925 | 4.4:-38846351 | None:intergenic |
| CCAATTGCACCTATTGGCAA+AGG | 0.511651 | 4.4:+38847474 | MS.gene40638:CDS |
| TCCCTGCCGCGGCGAGACAT+AGG | 0.512835 | 4.4:-38847194 | None:intergenic |
| ATCTTCACCGGTCACCAAGA+TGG | 0.520474 | 4.4:+38846598 | MS.gene40638:CDS |
| GGTGTGAAATAACGAGGGTT+CGG | 0.520624 | 4.4:-38846143 | None:intergenic |
| GTGTGGAGGCGTGACGATAG+AGG | 0.521361 | 4.4:+38847255 | MS.gene40638:CDS |
| CCACATCTTCACTGTTCCAT+CGG | 0.523320 | 4.4:-38846959 | None:intergenic |
| AACCATGGTGATTTGTTGAG+AGG | 0.523351 | 4.4:-38846304 | None:intergenic |
| TGTTCTAGGACTCGCGGCCG+AGG | 0.526893 | 4.4:-38846203 | None:intergenic |
| GGTGTTTCTCTCTACTAACA+TGG | 0.528485 | 4.4:-38846065 | None:intergenic |
| CTCCTCCTCGTGTTCTTCAA+CGG | 0.530483 | 4.4:-38847334 | None:intergenic |
| GGATTGAATTATTCTTCCCC+AGG | 0.531004 | 4.4:+38847495 | MS.gene40638:CDS |
| CAAAGAACTACGTAGAAGTC+CGG | 0.531424 | 4.4:+38846722 | MS.gene40638:CDS |
| AGGATGATCGCGATGATCAA+CGG | 0.538331 | 4.4:+38847355 | MS.gene40638:CDS |
| CGTTGAAGAACACGAGGAGG+AGG | 0.539002 | 4.4:+38847335 | MS.gene40638:CDS |
| CTAATTACATGTTATCTCCA+TGG | 0.539785 | 4.4:+38846263 | MS.gene40638:CDS |
| AAAGAACTTAAAGACTATAC+CGG | 0.540811 | 4.4:+38846523 | MS.gene40638:CDS |
| AGTTAAGGTTGATGCAAGGT+TGG | 0.541351 | 4.4:+38847445 | MS.gene40638:CDS |
| GGGTCACTAGCTCGCGAAGA+AGG | 0.547475 | 4.4:+38846427 | MS.gene40638:CDS |
| CATGTCACCGGAAACGGCTA+AGG | 0.551651 | 4.4:-38846461 | None:intergenic |
| GAAGGAGGGTTGTTGTACTC+AGG | 0.553403 | 4.4:+38846808 | MS.gene40638:CDS |
| ACCAACAACGATGATGATCA+AGG | 0.553556 | 4.4:+38846166 | MS.gene40638:CDS |
| TCACCGTGTTTCTACGTCGC+CGG | 0.558845 | 4.4:-38846741 | None:intergenic |
| AAAACCATTGTTATCTCCGA+AGG | 0.559695 | 4.4:+38846571 | MS.gene40638:CDS |
| TTGATCATCATCGTTGTTGG+TGG | 0.562709 | 4.4:-38846164 | None:intergenic |
| CTGAGTTTGGATCAAGAAGG+AGG | 0.576458 | 4.4:+38846793 | MS.gene40638:CDS |
| TCCGAAGGAAACATCTTCAC+CGG | 0.578285 | 4.4:+38846586 | MS.gene40638:CDS |
| CCTTGCATCAACCTTAACTC+TGG | 0.578888 | 4.4:-38847441 | None:intergenic |
| AGGCCGTATCAGCAACGCCT+CGG | 0.581907 | 4.4:+38846186 | MS.gene40638:CDS |
| GTTGTTGTACTCAGGTTCGT+GGG | 0.586838 | 4.4:+38846816 | MS.gene40638:CDS |
| GGTTTGAAATGCTGCAACCA+CGG | 0.587053 | 4.4:-38846917 | None:intergenic |
| TGAATGACAAACACAAAAGG+TGG | 0.588072 | 4.4:+38846040 | None:intergenic |
| TGTCGTTATAATTGTTGTCG+TGG | 0.588164 | 4.4:-38846378 | None:intergenic |
| ATAAGAATGTTTGCGTGTGG+AGG | 0.596565 | 4.4:+38847241 | MS.gene40638:CDS |
| TCATCAACGATTATTTATTG+CGG | 0.596640 | 4.4:+38847081 | MS.gene40638:CDS |
| CCGATGGAACAGTGAAGATG+TGG | 0.598551 | 4.4:+38846959 | MS.gene40638:CDS |
| GCTGACATTGATTGTTCCTG+GGG | 0.599863 | 4.4:-38847511 | None:intergenic |
| GAAACAAGAAAACGCTGTGA+CGG | 0.601740 | 4.4:+38847041 | MS.gene40638:CDS |
| TGTCGTGGCGGTGGCTGTGG+TGG | 0.601834 | 4.4:-38846363 | None:intergenic |
| CCGATAAGAATGTTTGCGTG+TGG | 0.609682 | 4.4:+38847238 | MS.gene40638:CDS |
| TTAAAACACCGCCGTGAGTG+AGG | 0.611554 | 4.4:-38847152 | None:intergenic |
| TGAGTTTGGATCAAGAAGGA+GGG | 0.624574 | 4.4:+38846794 | MS.gene40638:CDS |
| ACCGGTGTATAACATGTCAC+CGG | 0.625134 | 4.4:-38846473 | None:intergenic |
| ATGGAACAGTGAAGATGTGG+AGG | 0.633593 | 4.4:+38846962 | MS.gene40638:CDS |
| GTTTCCTTCGGAGATAACAA+TGG | 0.634476 | 4.4:-38846575 | None:intergenic |
| TCCGGTGACATGTTATACAC+CGG | 0.639848 | 4.4:+38846472 | MS.gene40638:CDS |
| CAGAAGAATAACCTCACTCA+CGG | 0.641267 | 4.4:+38847141 | MS.gene40638:CDS |
| CCAGAGTTAAGGTTGATGCA+AGG | 0.650036 | 4.4:+38847441 | MS.gene40638:CDS |
| GTGGAGGCGTGACGATAGAG+GGG | 0.652212 | 4.4:+38847257 | MS.gene40638:CDS |
| AGTCTGAGTTTGGATCAAGA+AGG | 0.656181 | 4.4:+38846790 | MS.gene40638:CDS |
| GCGACGTAGAAACACGGTGA+AGG | 0.656210 | 4.4:+38846744 | MS.gene40638:CDS |
| CGCGAGCTAGTGACCCGATG+AGG | 0.657515 | 4.4:-38846420 | None:intergenic |
| CACGCAAACATTCTTATCGG+CGG | 0.663813 | 4.4:-38847235 | None:intergenic |
| CATTGCCGTTGAAGAACACG+AGG | 0.665220 | 4.4:+38847329 | MS.gene40638:CDS |
| TGCCGTTGAAGAACACGAGG+AGG | 0.667001 | 4.4:+38847332 | MS.gene40638:CDS |
| GAAATATGTTCTAGGACTCG+CGG | 0.670435 | 4.4:-38846209 | None:intergenic |
| CGCAGTCCTATGTCTCGCCG+CGG | 0.677705 | 4.4:+38847188 | MS.gene40638:CDS |
| ATGTGGAGGAGAGACATCGA+CGG | 0.680232 | 4.4:+38846976 | MS.gene40638:CDS |
| CTCCTCTCAACAAATCACCA+TGG | 0.687284 | 4.4:+38846302 | MS.gene40638:CDS |
| CGTTATAATTGTTGTCGTGG+CGG | 0.693088 | 4.4:-38846375 | None:intergenic |
| GTTGGTGGTGTGAAATAACG+AGG | 0.700463 | 4.4:-38846149 | None:intergenic |
| TCTGATGATGGCATTAACCA+TGG | 0.701204 | 4.4:-38846319 | None:intergenic |
| AGTCCGGCGACGTAGAAACA+CGG | 0.702669 | 4.4:+38846738 | MS.gene40638:CDS |
| ATGATCGCGATGATCAACGG+TGG | 0.708309 | 4.4:+38847358 | MS.gene40638:CDS |
| GAAAACAAGATTCCCTGCCG+CGG | 0.715565 | 4.4:-38847205 | None:intergenic |
| GGCTGAGCCGGTGAAAACGT+AGG | 0.729585 | 4.4:-38846938 | None:intergenic |
| TTGGTGGTGTGAAATAACGA+GGG | 0.733290 | 4.4:-38846148 | None:intergenic |
| TGATGACGCAGTGAACGCCG+TGG | 0.743023 | 4.4:+38846900 | MS.gene40638:CDS |
| AAGAATAACCTCACTCACGG+CGG | 0.746398 | 4.4:+38847144 | MS.gene40638:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATACATAGACCAAAATT+TGG | + | chr4.4:38846106-38846125 | MS.gene40638:CDS | 20.0% |
| !!! | AATAAATAATCGTTGATGAA+TGG | - | chr4.4:38847080-38847099 | None:intergenic | 20.0% |
| !!! | TTTATTGGTGCTATTATTAT+TGG | - | chr4.4:38846089-38846108 | None:intergenic | 20.0% |
| !!! | TTTTGGTCTATGTATTTTAT+TGG | - | chr4.4:38846104-38846123 | None:intergenic | 20.0% |
| ! | AAAGAACTTAAAGACTATAC+CGG | + | chr4.4:38846523-38846542 | MS.gene40638:CDS | 25.0% |
| ! | TCATCAACGATTATTTATTG+CGG | + | chr4.4:38847081-38847100 | MS.gene40638:CDS | 25.0% |
| !! | TTGAAGAAATTTTCCAAACT+CGG | - | chr4.4:38846630-38846649 | None:intergenic | 25.0% |
| CATGGAGATAACATGTAATT+AGG | - | chr4.4:38846265-38846284 | None:intergenic | 30.0% | |
| CTAATTACATGTTATCTCCA+TGG | + | chr4.4:38846263-38846282 | MS.gene40638:CDS | 30.0% | |
| GATAGTTTATACTGGTAGTT+TGG | + | chr4.4:38847380-38847399 | MS.gene40638:CDS | 30.0% | |
| TAATTCAATCCTTTGCCAAT+AGG | - | chr4.4:38847486-38847505 | None:intergenic | 30.0% | |
| TACCGATAGCAAAAACATAA+GGG | + | chr4.4:38846495-38846514 | MS.gene40638:CDS | 30.0% | |
| TAGTTTGGATAAGTCTGTTA+AGG | + | chr4.4:38847395-38847414 | MS.gene40638:CDS | 30.0% | |
| TCAGCAACAATAGTAATAGA+AGG | + | chr4.4:38847529-38847548 | MS.gene40638:CDS | 30.0% | |
| TCTAGAAATCATAAGCGTAT+TGG | + | chr4.4:38846655-38846674 | MS.gene40638:CDS | 30.0% | |
| ! | AATCGTTGATGAATGGTTAA+CGG | - | chr4.4:38847073-38847092 | None:intergenic | 30.0% |
| ! | TTGACATAGTCTTTGAAAGT+TGG | - | chr4.4:38846688-38846707 | None:intergenic | 30.0% |
| ! | TTTTAAATCAAGCAGTGGTT+TGG | + | chr4.4:38846546-38846565 | MS.gene40638:CDS | 30.0% |
| !!! | TGTAGAAAAGCACCAAATTT+TGG | - | chr4.4:38846121-38846140 | None:intergenic | 30.0% |
| AAAACCATTGTTATCTCCGA+AGG | + | chr4.4:38846571-38846590 | MS.gene40638:CDS | 35.0% | |
| AAGATGGAAAAATCCGAGTT+TGG | + | chr4.4:38846614-38846633 | MS.gene40638:CDS | 35.0% | |
| AATTCTCCAATTGCACCTAT+TGG | + | chr4.4:38847468-38847487 | MS.gene40638:CDS | 35.0% | |
| ATAGCAAAAACATAAGGGTG+TGG | + | chr4.4:38846500-38846519 | MS.gene40638:CDS | 35.0% | |
| ATTTATTGCGGTTCTTCTGA+TGG | + | chr4.4:38847093-38847112 | MS.gene40638:CDS | 35.0% | |
| GGGATAAAACTCTCAAAGTT+TGG | + | chr4.4:38846836-38846855 | MS.gene40638:CDS | 35.0% | |
| TGTCGTTATAATTGTTGTCG+TGG | - | chr4.4:38846381-38846400 | None:intergenic | 35.0% | |
| ! | AAACTCGGATTTTTCCATCT+TGG | - | chr4.4:38846615-38846634 | None:intergenic | 35.0% |
| ! | ACCACTGCTTGATTTAAAAC+CGG | - | chr4.4:38846545-38846564 | None:intergenic | 35.0% |
| ! | CATTTGTCTTTCCGTTTTGA+CGG | + | chr4.4:38847284-38847303 | MS.gene40638:CDS | 35.0% |
| ! | GAAGAAAACGAAGCATGTTT+TGG | + | chr4.4:38847005-38847024 | MS.gene40638:CDS | 35.0% |
| ! | GTACCGATAGCAAAAACATA+AGG | + | chr4.4:38846494-38846513 | MS.gene40638:CDS | 35.0% |
| ! | TTATAACGACAGCTATTTCC+CGG | + | chr4.4:38846390-38846409 | MS.gene40638:CDS | 35.0% |
| ! | TTTCAAACCTACGTTTTCAC+CGG | + | chr4.4:38846931-38846950 | MS.gene40638:CDS | 35.0% |
| !! | AAACGAAGCATGTTTTGGAT+AGG | + | chr4.4:38847010-38847029 | MS.gene40638:CDS | 35.0% |
| !! | ATTTGTCTTTCCGTTTTGAC+GGG | + | chr4.4:38847285-38847304 | MS.gene40638:CDS | 35.0% |
| !! | CACCCTTATGTTTTTGCTAT+CGG | - | chr4.4:38846500-38846519 | None:intergenic | 35.0% |
| !! | TATGTTTTTGCTATCGGTAC+CGG | - | chr4.4:38846494-38846513 | None:intergenic | 35.0% |
| !! | TGATGGATTGGTTAACTTCT+GGG | + | chr4.4:38847110-38847129 | MS.gene40638:CDS | 35.0% |
| ACCAACAACGATGATGATCA+AGG | + | chr4.4:38846166-38846185 | MS.gene40638:CDS | 40.0% | |
| AGTCTGAGTTTGGATCAAGA+AGG | + | chr4.4:38846790-38846809 | MS.gene40638:CDS | 40.0% | |
| ATAAGAATGTTTGCGTGTGG+AGG | + | chr4.4:38847241-38847260 | MS.gene40638:CDS | 40.0% | |
| CAAAGAACTACGTAGAAGTC+CGG | + | chr4.4:38846722-38846741 | MS.gene40638:CDS | 40.0% | |
| CAACGGTGGATAGTTTATAC+TGG | + | chr4.4:38847372-38847391 | MS.gene40638:CDS | 40.0% | |
| CAGAAGAATAACCTCACTCA+CGG | + | chr4.4:38847141-38847160 | MS.gene40638:CDS | 40.0% | |
| CCACACGCAAACATTCTTAT+CGG | - | chr4.4:38847241-38847260 | None:intergenic | 40.0% | |
| CGTTATAATTGTTGTCGTGG+CGG | - | chr4.4:38846378-38846397 | None:intergenic | 40.0% | |
| GAAACAAGAAAACGCTGTGA+CGG | + | chr4.4:38847041-38847060 | MS.gene40638:CDS | 40.0% | |
| GAAGAAGTTTCTTGGTTCCA+TGG | - | chr4.4:38846283-38846302 | None:intergenic | 40.0% | |
| GCGAGTCCTAGAACATATTT+CGG | + | chr4.4:38846211-38846230 | MS.gene40638:CDS | 40.0% | |
| GGATTGAATTATTCTTCCCC+AGG | + | chr4.4:38847495-38847514 | MS.gene40638:CDS | 40.0% | |
| GGTGTTTCTCTCTACTAACA+TGG | - | chr4.4:38846068-38846087 | None:intergenic | 40.0% | |
| GTATAACATGTCACCGGAAA+CGG | - | chr4.4:38846470-38846489 | None:intergenic | 40.0% | |
| GTTTCCTTCGGAGATAACAA+TGG | - | chr4.4:38846578-38846597 | None:intergenic | 40.0% | |
| TGAGAGGAGAAGAAGTTTCT+TGG | - | chr4.4:38846291-38846310 | None:intergenic | 40.0% | |
| TGAGTTTGGATCAAGAAGGA+GGG | + | chr4.4:38846794-38846813 | MS.gene40638:CDS | 40.0% | |
| TGGATAAGTCTGTTAAGGTC+TGG | + | chr4.4:38847400-38847419 | MS.gene40638:CDS | 40.0% | |
| TTCAACGGCAATGCATTTGA+CGG | - | chr4.4:38847322-38847341 | None:intergenic | 40.0% | |
| TTGATCATCATCGTTGTTGG+TGG | - | chr4.4:38846167-38846186 | None:intergenic | 40.0% | |
| ! | AGGTTGATTGCTTCTGATGA+TGG | - | chr4.4:38846334-38846353 | None:intergenic | 40.0% |
| ! | CGTTACGGATTTCAAGTGTA+TGG | + | chr4.4:38846861-38846880 | MS.gene40638:CDS | 40.0% |
| ! | TGCTGACATTGATTGTTCCT+GGG | - | chr4.4:38847515-38847534 | None:intergenic | 40.0% |
| ! | TTGCTGACATTGATTGTTCC+TGG | - | chr4.4:38847516-38847535 | None:intergenic | 40.0% |
| !! | AACCATGGTGATTTGTTGAG+AGG | - | chr4.4:38846307-38846326 | None:intergenic | 40.0% |
| !! | AGTTAAGGTTGATGCAAGGT+TGG | + | chr4.4:38847445-38847464 | MS.gene40638:CDS | 40.0% |
| !! | CTGATGGATTGGTTAACTTC+TGG | + | chr4.4:38847109-38847128 | MS.gene40638:CDS | 40.0% |
| !! | GAAATATGTTCTAGGACTCG+CGG | - | chr4.4:38846212-38846231 | None:intergenic | 40.0% |
| !! | GATTTTTCCATCTTGGTGAC+CGG | - | chr4.4:38846608-38846627 | None:intergenic | 40.0% |
| !! | TCTGATGATGGCATTAACCA+TGG | - | chr4.4:38846322-38846341 | None:intergenic | 40.0% |
| !! | TTGGTGGTGTGAAATAACGA+GGG | - | chr4.4:38846151-38846170 | None:intergenic | 40.0% |
| !!! | ACCGGTTTTAAATCAAGCAG+TGG | + | chr4.4:38846541-38846560 | MS.gene40638:CDS | 40.0% |
| AAGAATAACCTCACTCACGG+CGG | + | chr4.4:38847144-38847163 | MS.gene40638:CDS | 45.0% | |
| ACCGGTGAAGATGTTTCCTT+CGG | - | chr4.4:38846590-38846609 | None:intergenic | 45.0% | |
| ACCGGTGTATAACATGTCAC+CGG | - | chr4.4:38846476-38846495 | None:intergenic | 45.0% | |
| AGGATGATCGCGATGATCAA+CGG | + | chr4.4:38847355-38847374 | MS.gene40638:CDS | 45.0% | |
| ATCTACTCCTTAGCCGTTTC+CGG | + | chr4.4:38846454-38846473 | MS.gene40638:CDS | 45.0% | |
| ATGGAACAGTGAAGATGTGG+AGG | + | chr4.4:38846962-38846981 | MS.gene40638:CDS | 45.0% | |
| CACGCAAACATTCTTATCGG+CGG | - | chr4.4:38847238-38847257 | None:intergenic | 45.0% | |
| CCAATTGCACCTATTGGCAA+AGG | + | chr4.4:38847474-38847493 | MS.gene40638:CDS | 45.0% | |
| CCACATCTTCACTGTTCCAT+CGG | - | chr4.4:38846962-38846981 | None:intergenic | 45.0% | |
| CCGATAAGAATGTTTGCGTG+TGG | + | chr4.4:38847238-38847257 | MS.gene40638:CDS | 45.0% | |
| CCTTGCATCAACCTTAACTC+TGG | - | chr4.4:38847444-38847463 | None:intergenic | 45.0% | |
| CCTTTGCCAATAGGTGCAAT+TGG | - | chr4.4:38847477-38847496 | None:intergenic | 45.0% | |
| CGGACTTCTACGTAGTTCTT+TGG | - | chr4.4:38846724-38846743 | None:intergenic | 45.0% | |
| CTCCTCTCAACAAATCACCA+TGG | + | chr4.4:38846302-38846321 | MS.gene40638:CDS | 45.0% | |
| CTGAGTTTGGATCAAGAAGG+AGG | + | chr4.4:38846793-38846812 | MS.gene40638:CDS | 45.0% | |
| CTGTCGCCGAAATATGTTCT+AGG | - | chr4.4:38846220-38846239 | None:intergenic | 45.0% | |
| GCCTTGATCATCATCGTTGT+TGG | - | chr4.4:38846170-38846189 | None:intergenic | 45.0% | |
| GGTGTGAAATAACGAGGGTT+CGG | - | chr4.4:38846146-38846165 | None:intergenic | 45.0% | |
| GTTGTTGTACTCAGGTTCGT+GGG | + | chr4.4:38846816-38846835 | MS.gene40638:CDS | 45.0% | |
| TATAATTGTTGTCGTGGCGG+TGG | - | chr4.4:38846375-38846394 | None:intergenic | 45.0% | |
| TCAACGGCAATGCATTTGAC+GGG | - | chr4.4:38847321-38847340 | None:intergenic | 45.0% | |
| TCCGAAGGAAACATCTTCAC+CGG | + | chr4.4:38846586-38846605 | MS.gene40638:CDS | 45.0% | |
| TCCGGTGACATGTTATACAC+CGG | + | chr4.4:38846472-38846491 | MS.gene40638:CDS | 45.0% | |
| TCTCAAAGTTTGGCGCGTTA+CGG | + | chr4.4:38846846-38846865 | MS.gene40638:CDS | 45.0% | |
| ! | GACAGCTATTTCCCGGAAAA+TGG | + | chr4.4:38846397-38846416 | MS.gene40638:CDS | 45.0% |
| ! | GCTGACATTGATTGTTCCTG+GGG | - | chr4.4:38847514-38847533 | None:intergenic | 45.0% |
| ! | GGTTTGAAATGCTGCAACCA+CGG | - | chr4.4:38846920-38846939 | None:intergenic | 45.0% |
| ! | TTGCGGTTCTTCTGATGGAT+TGG | + | chr4.4:38847098-38847117 | MS.gene40638:CDS | 45.0% |
| !! | ACTCACGGCGGTGTTTTAAA+GGG | + | chr4.4:38847156-38847175 | MS.gene40638:CDS | 45.0% |
| !! | CCAGAGTTAAGGTTGATGCA+AGG | + | chr4.4:38847441-38847460 | MS.gene40638:CDS | 45.0% |
| !! | GTTGGTGGTGTGAAATAACG+AGG | - | chr4.4:38846152-38846171 | None:intergenic | 45.0% |
| !! | TGCTGTTTCGAGTCTGAGTT+TGG | + | chr4.4:38846780-38846799 | MS.gene40638:CDS | 45.0% |
| !!! | GCAGGGAATCTTGTTTTCAG+TGG | + | chr4.4:38847210-38847229 | MS.gene40638:CDS | 45.0% |
| ACCAGTGTGTCCCGTCAAAA+CGG | - | chr4.4:38847298-38847317 | None:intergenic | 50.0% | |
| AGGAGAGACATCGACGGAAA+AGG | + | chr4.4:38846982-38847001 | MS.gene40638:CDS | 50.0% | |
| ATCTTCACCGGTCACCAAGA+TGG | + | chr4.4:38846598-38846617 | MS.gene40638:CDS | 50.0% | |
| ATGATCGCGATGATCAACGG+TGG | + | chr4.4:38847358-38847377 | MS.gene40638:CDS | 50.0% | |
| ATGTGGAGGAGAGACATCGA+CGG | + | chr4.4:38846976-38846995 | MS.gene40638:CDS | 50.0% | |
| CATTGCCGTTGAAGAACACG+AGG | + | chr4.4:38847329-38847348 | MS.gene40638:CDS | 50.0% | |
| CCGATGGAACAGTGAAGATG+TGG | + | chr4.4:38846959-38846978 | MS.gene40638:CDS | 50.0% | |
| CTCCTCCTCGTGTTCTTCAA+CGG | - | chr4.4:38847337-38847356 | None:intergenic | 50.0% | |
| CTGAACATGCGCCAGAGTTA+AGG | + | chr4.4:38847430-38847449 | MS.gene40638:CDS | 50.0% | |
| GAAAACAAGATTCCCTGCCG+CGG | - | chr4.4:38847208-38847227 | None:intergenic | 50.0% | |
| GAAGGAGGGTTGTTGTACTC+AGG | + | chr4.4:38846808-38846827 | MS.gene40638:CDS | 50.0% | |
| GGAGAGACATCGACGGAAAA+GGG | + | chr4.4:38846983-38847002 | MS.gene40638:CDS | 50.0% | |
| GGTTGTTGTACTCAGGTTCG+TGG | + | chr4.4:38846815-38846834 | MS.gene40638:CDS | 50.0% | |
| TTAAAACACCGCCGTGAGTG+AGG | - | chr4.4:38847155-38847174 | None:intergenic | 50.0% | |
| TTCCCGGAAAATGGCCTCAT+CGG | + | chr4.4:38846406-38846425 | MS.gene40638:CDS | 50.0% | |
| !! | CACTCACGGCGGTGTTTTAA+AGG | + | chr4.4:38847155-38847174 | MS.gene40638:CDS | 50.0% |
| AGTCCGGCGACGTAGAAACA+CGG | + | chr4.4:38846738-38846757 | MS.gene40638:CDS | 55.0% | |
| CATGTCACCGGAAACGGCTA+AGG | - | chr4.4:38846464-38846483 | None:intergenic | 55.0% | |
| CGTTGAAGAACACGAGGAGG+AGG | + | chr4.4:38847335-38847354 | MS.gene40638:CDS | 55.0% | |
| GCGACGTAGAAACACGGTGA+AGG | + | chr4.4:38846744-38846763 | MS.gene40638:CDS | 55.0% | |
| TCCCGGAAAATGGCCTCATC+GGG | + | chr4.4:38846407-38846426 | MS.gene40638:CDS | 55.0% | |
| TGCCGTTGAAGAACACGAGG+AGG | + | chr4.4:38847332-38847351 | MS.gene40638:CDS | 55.0% | |
| ! | GGCTGTGGTGGTATTGGAAG+AGG | - | chr4.4:38846354-38846373 | None:intergenic | 55.0% |
| ! | TCACCGTGTTTCTACGTCGC+CGG | - | chr4.4:38846744-38846763 | None:intergenic | 55.0% |
| ! | TGTGGAGGCGTGACGATAGA+GGG | + | chr4.4:38847256-38847275 | MS.gene40638:CDS | 55.0% |
| !! | ACCCGATGAGGCCATTTTCC+GGG | - | chr4.4:38846411-38846430 | None:intergenic | 55.0% |
| !! | GACCCGATGAGGCCATTTTC+CGG | - | chr4.4:38846412-38846431 | None:intergenic | 55.0% |
| !! | TCCGTTTTGACGGGACACAC+TGG | + | chr4.4:38847294-38847313 | MS.gene40638:CDS | 55.0% |
| AGGCCGTATCAGCAACGCCT+CGG | + | chr4.4:38846186-38846205 | MS.gene40638:CDS | 60.0% | |
| GGCTGAGCCGGTGAAAACGT+AGG | - | chr4.4:38846941-38846960 | None:intergenic | 60.0% | |
| GGGTCACTAGCTCGCGAAGA+AGG | + | chr4.4:38846427-38846446 | MS.gene40638:CDS | 60.0% | |
| ! | CACTGTTCCATCGGCTGAGC+CGG | - | chr4.4:38846953-38846972 | None:intergenic | 60.0% |
| ! | GTGGAGGCGTGACGATAGAG+GGG | + | chr4.4:38847257-38847276 | MS.gene40638:CDS | 60.0% |
| ! | GTGTGGAGGCGTGACGATAG+AGG | + | chr4.4:38847255-38847274 | MS.gene40638:CDS | 60.0% |
| ! | TGATGACGCAGTGAACGCCG+TGG | + | chr4.4:38846900-38846919 | MS.gene40638:CDS | 60.0% |
| !!! | GTTTTCACCGGCTCAGCCGA+TGG | + | chr4.4:38846943-38846962 | MS.gene40638:CDS | 60.0% |
| CGCAGTCCTATGTCTCGCCG+CGG | + | chr4.4:38847188-38847207 | MS.gene40638:CDS | 65.0% | |
| CGCGAGCTAGTGACCCGATG+AGG | - | chr4.4:38846423-38846442 | None:intergenic | 65.0% | |
| GGCGGTGGCTGTGGTGGTAT+TGG | - | chr4.4:38846360-38846379 | None:intergenic | 65.0% | |
| TCCTATGTCTCGCCGCGGCA+GGG | + | chr4.4:38847193-38847212 | MS.gene40638:CDS | 65.0% | |
| TGTTGTCGTGGCGGTGGCTG+TGG | - | chr4.4:38846369-38846388 | None:intergenic | 65.0% | |
| !! | CGGCCGAGGCGTTGCTGATA+CGG | - | chr4.4:38846192-38846211 | None:intergenic | 65.0% |
| !! | TGTTCTAGGACTCGCGGCCG+AGG | - | chr4.4:38846206-38846225 | None:intergenic | 65.0% |
| GTCCTATGTCTCGCCGCGGC+AGG | + | chr4.4:38847192-38847211 | MS.gene40638:CDS | 70.0% | |
| TCCCTGCCGCGGCGAGACAT+AGG | - | chr4.4:38847197-38847216 | None:intergenic | 70.0% | |
| TGTCGTGGCGGTGGCTGTGG+TGG | - | chr4.4:38846366-38846385 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 38846043 | 38847566 | 38846043 | ID=MS.gene40638 |
| chr4.4 | mRNA | 38846043 | 38847566 | 38846043 | ID=MS.gene40638.t1;Parent=MS.gene40638 |
| chr4.4 | exon | 38846043 | 38847566 | 38846043 | ID=MS.gene40638.t1.exon1;Parent=MS.gene40638.t1 |
| chr4.4 | CDS | 38846043 | 38847566 | 38846043 | ID=cds.MS.gene40638.t1;Parent=MS.gene40638.t1 |
| Gene Sequence |
| Protein sequence |
