Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41064.t1 | XP_019438836.1 | 63.5 | 63 | 23 | 0 | 54 | 116 | 141 | 203 | 8.50E-16 | 93.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41064.t1 | A0A445GI25 | 68.3 | 63 | 17 | 1 | 53 | 112 | 113 | 175 | 2.2e-13 | 84.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051125 | MS.gene41064 | 0.810422 | 1.16E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTCACTCTTCAAAA+TGG | 0.170935 | 1.3:+42083676 | None:intergenic |
| AATTTGAACTCAACAGGAAA+TGG | 0.399868 | 1.3:-42084147 | MS.gene41064:CDS |
| CATCTGAATTTGAACTCAAC+AGG | 0.409257 | 1.3:-42084153 | MS.gene41064:CDS |
| AAGATCTTACATTCATCATC+AGG | 0.417940 | 1.3:+42083805 | None:intergenic |
| TCAGCTTCACTCTTCAAAAT+GGG | 0.432393 | 1.3:+42083677 | None:intergenic |
| GAGTGAGACCAGTAGTAGTT+TGG | 0.451269 | 1.3:+42084069 | None:intergenic |
| CTCATCGTAGTCGACTTCAT+TGG | 0.458097 | 1.3:-42084045 | MS.gene41064:CDS |
| CTATGGAGCAAGGAGAATAA+TGG | 0.470206 | 1.3:-42083986 | MS.gene41064:intron |
| GGATAATGTTGGTAATTCTC+AGG | 0.478704 | 1.3:-42084120 | MS.gene41064:CDS |
| CTTTGATGTGTGCTAGAAGT+TGG | 0.519354 | 1.3:-42084009 | MS.gene41064:CDS |
| ACTTCATTGGACAACCTTGG+AGG | 0.521822 | 1.3:-42084032 | MS.gene41064:CDS |
| AGTGCACACCAAACTACTAC+TGG | 0.530092 | 1.3:-42084077 | MS.gene41064:CDS |
| TCGACTTCATTGGACAACCT+TGG | 0.544964 | 1.3:-42084035 | MS.gene41064:CDS |
| TAGAAGTTGGCTATGGAGCA+AGG | 0.597997 | 1.3:-42083996 | MS.gene41064:CDS |
| GAAATGGACATGGATAATGT+TGG | 0.622790 | 1.3:-42084131 | MS.gene41064:CDS |
| AACTCAACAGGAAATGGACA+TGG | 0.624712 | 1.3:-42084141 | MS.gene41064:CDS |
| AGCACACATCAAAGCCTCCA+AGG | 0.630649 | 1.3:+42084018 | None:intergenic |
| CAATGAAGTCGACTACGATG+AGG | 0.638748 | 1.3:+42084046 | None:intergenic |
| TGTGTGCTAGAAGTTGGCTA+TGG | 0.638996 | 1.3:-42084003 | MS.gene41064:CDS |
| AATGAAGTCGACTACGATGA+GGG | 0.641996 | 1.3:+42084047 | None:intergenic |
| ATAGGTGAAATATTGACTAG+TGG | 0.646439 | 1.3:-42083708 | MS.gene41064:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAAAAATTCAGAAAAA+GGG | + | chr1.3:42083745-42083764 | None:intergenic | 10.0% |
| !!! | ACTTGTATTTTTTATTATAT+AGG | - | chr1.3:42084393-42084412 | MS.gene41064:intron | 10.0% |
| !! | AAACATTAAAGTTAGTAAAT+TGG | + | chr1.3:42084365-42084384 | None:intergenic | 15.0% |
| !! | CATATAAAAAATTCAGAAAA+AGG | + | chr1.3:42083746-42083765 | None:intergenic | 15.0% |
| !! | ATTAAAGTTAGTAAATTGGA+AGG | + | chr1.3:42084361-42084380 | None:intergenic | 20.0% |
| !! | CTTTCAATTTACATATCTTA+AGG | - | chr1.3:42084173-42084192 | MS.gene41064:CDS | 20.0% |
| !!! | TTATTATTTTGTAGTGTTTG+TGG | - | chr1.3:42083880-42083899 | MS.gene41064:intron | 20.0% |
| ! | GGAAGGTAGTATTAAATAAA+TGG | + | chr1.3:42084344-42084363 | None:intergenic | 25.0% |
| !! | ATGTTATTGATGATTGAGAT+TGG | - | chr1.3:42083791-42083810 | MS.gene41064:intron | 25.0% |
| !!! | TTTTCTGCTTATTTTCTTCT+CGG | - | chr1.3:42083690-42083709 | MS.gene41064:CDS | 25.0% |
| !!! | TTTTTATATGTTCCGATTTG+TGG | - | chr1.3:42083756-42083775 | MS.gene41064:intron | 25.0% |
| AAGATCTTACATTCATCATC+AGG | + | chr1.3:42084317-42084336 | None:intergenic | 30.0% | |
| AATTTGAACTCAACAGGAAA+TGG | - | chr1.3:42083972-42083991 | MS.gene41064:intron | 30.0% | |
| ACTGCATTTGCATTATTTAC+AGG | - | chr1.3:42084236-42084255 | MS.gene41064:intron | 30.0% | |
| ! | ATAGGTGAAATATTGACTAG+TGG | - | chr1.3:42084411-42084430 | MS.gene41064:intron | 30.0% |
| CATCTGAATTTGAACTCAAC+AGG | - | chr1.3:42083966-42083985 | MS.gene41064:intron | 35.0% | |
| CTGTTGGATTTGAATGGTTA+TGG | - | chr1.3:42083839-42083858 | MS.gene41064:CDS | 35.0% | |
| GAAATGGACATGGATAATGT+TGG | - | chr1.3:42083988-42084007 | MS.gene41064:CDS | 35.0% | |
| TTGGATCTGTTGGATTTGAA+TGG | - | chr1.3:42083833-42083852 | MS.gene41064:CDS | 35.0% | |
| ! | TATGTTCCGATTTGTGGAAT+CGG | - | chr1.3:42083762-42083781 | MS.gene41064:intron | 35.0% |
| ! | TTTGAATGGTTATGGCTTGT+AGG | - | chr1.3:42083847-42083866 | MS.gene41064:CDS | 35.0% |
| !! | GGATAATGTTGGTAATTCTC+AGG | - | chr1.3:42083999-42084018 | MS.gene41064:CDS | 35.0% |
| !!! | GGTTCGTTTTGATTTTGTAG+TGG | - | chr1.3:42083901-42083920 | MS.gene41064:intron | 35.0% |
| AACTCAACAGGAAATGGACA+TGG | - | chr1.3:42083978-42083997 | MS.gene41064:intron | 40.0% | |
| AATGAAGTCGACTACGATGA+GGG | + | chr1.3:42084075-42084094 | None:intergenic | 40.0% | |
| ATCAGTTGTTTGTGTGTGGT+TGG | - | chr1.3:42083814-42083833 | MS.gene41064:intron | 40.0% | |
| CTATGGAGCAAGGAGAATAA+TGG | - | chr1.3:42084133-42084152 | MS.gene41064:CDS | 40.0% | |
| CTTTGATGTGTGCTAGAAGT+TGG | - | chr1.3:42084110-42084129 | MS.gene41064:CDS | 40.0% | |
| TTGGATCAGTTGTTTGTGTG+TGG | - | chr1.3:42083810-42083829 | MS.gene41064:intron | 40.0% | |
| ! | ATGGTTATGGCTTGTAGGTA+CGG | - | chr1.3:42083852-42083871 | MS.gene41064:CDS | 40.0% |
| ACTTCATTGGACAACCTTGG+AGG | - | chr1.3:42084087-42084106 | MS.gene41064:CDS | 45.0% | |
| AGTGCACACCAAACTACTAC+TGG | - | chr1.3:42084042-42084061 | MS.gene41064:CDS | 45.0% | |
| CAATGAAGTCGACTACGATG+AGG | + | chr1.3:42084076-42084095 | None:intergenic | 45.0% | |
| GAGTGAGACCAGTAGTAGTT+TGG | + | chr1.3:42084053-42084072 | None:intergenic | 45.0% | |
| TAGAAGTTGGCTATGGAGCA+AGG | - | chr1.3:42084123-42084142 | MS.gene41064:CDS | 45.0% | |
| TCGACTTCATTGGACAACCT+TGG | - | chr1.3:42084084-42084103 | MS.gene41064:CDS | 45.0% | |
| TGTGTGCTAGAAGTTGGCTA+TGG | - | chr1.3:42084116-42084135 | MS.gene41064:CDS | 45.0% | |
| TTGTGTGTGGTTGGATCTGT+TGG | - | chr1.3:42083823-42083842 | MS.gene41064:CDS | 45.0% | |
| ! | CACTGACCGATTCCACAAAT+CGG | + | chr1.3:42083771-42083790 | None:intergenic | 45.0% |
| ! | CTCATCGTAGTCGACTTCAT+TGG | - | chr1.3:42084074-42084093 | MS.gene41064:CDS | 45.0% |
| ! | TATGGCTTGTAGGTACGGTT+TGG | - | chr1.3:42083857-42083876 | MS.gene41064:CDS | 45.0% |
| AGCACACATCAAAGCCTCCA+AGG | + | chr1.3:42084104-42084123 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 42083683 | 42084458 | 42083683 | ID=MS.gene41064 |
| chr1.3 | mRNA | 42083683 | 42084458 | 42083683 | ID=MS.gene41064.t1;Parent=MS.gene41064 |
| chr1.3 | exon | 42084455 | 42084458 | 42084455 | ID=MS.gene41064.t1.exon1;Parent=MS.gene41064.t1 |
| chr1.3 | CDS | 42084455 | 42084458 | 42084455 | ID=cds.MS.gene41064.t1;Parent=MS.gene41064.t1 |
| chr1.3 | exon | 42083987 | 42084220 | 42083987 | ID=MS.gene41064.t1.exon2;Parent=MS.gene41064.t1 |
| chr1.3 | CDS | 42083987 | 42084220 | 42083987 | ID=cds.MS.gene41064.t1;Parent=MS.gene41064.t1 |
| chr1.3 | exon | 42083815 | 42083883 | 42083815 | ID=MS.gene41064.t1.exon3;Parent=MS.gene41064.t1 |
| chr1.3 | CDS | 42083815 | 42083883 | 42083815 | ID=cds.MS.gene41064.t1;Parent=MS.gene41064.t1 |
| chr1.3 | exon | 42083683 | 42083726 | 42083683 | ID=MS.gene41064.t1.exon4;Parent=MS.gene41064.t1 |
| chr1.3 | CDS | 42083683 | 42083726 | 42083683 | ID=cds.MS.gene41064.t1;Parent=MS.gene41064.t1 |
| Gene Sequence |
| Protein sequence |
