Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051125.t1 | KYP47222.1 | 68.8 | 170 | 51 | 2 | 60 | 228 | 7 | 175 | 8.30E-59 | 237.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051125.t1 | P08770 | 34.4 | 180 | 98 | 5 | 68 | 234 | 141 | 313 | 6.7e-17 | 89.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051125.t1 | A0A151RXE3 | 68.8 | 170 | 51 | 2 | 60 | 228 | 7 | 175 | 6.0e-59 | 237.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051125 | MS.gene056764 | 0.802784 | 4.76E-49 | -1.69E-46 |
| MS.gene051125 | MS.gene41064 | 0.810422 | 1.16E-50 | -1.69E-46 |
| MS.gene051125 | MS.gene41974 | 0.828647 | 8.00E-55 | -1.69E-46 |
| MS.gene051125 | MS.gene45406 | 0.813981 | 1.94E-51 | -1.69E-46 |
| MS.gene051125 | MS.gene49175 | 0.804908 | 1.72E-49 | -1.69E-46 |
| MS.gene051125 | MS.gene80438 | 0.831699 | 1.44E-55 | -1.69E-46 |
| MS.gene051125 | MS.gene84787 | 0.831908 | 1.28E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051125.t1 | MTR_4g036325 | 52.055 | 73 | 34 | 1 | 157 | 228 | 2 | 74 | 9.94e-19 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051125.t1 | AT3G42170 | 27.861 | 201 | 126 | 6 | 45 | 226 | 42 | 242 | 2.04e-16 | 79.0 |
| MS.gene051125.t1 | AT3G42170 | 27.861 | 201 | 126 | 6 | 45 | 226 | 20 | 220 | 2.41e-16 | 79.0 |
Find 54 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCACGAAGCTTAAAGTTAA+TGG | 0.121499 | 8.3:-70381940 | MS.gene051125:CDS |
| TTCAGCTTCACTCTTCAAAA+TGG | 0.177755 | 8.3:+70381063 | None:intergenic |
| CAAGAAGTTTCTTGCAATAA+TGG | 0.264840 | 8.3:+70381899 | None:intergenic |
| CATTATTGCAAGAAACTTCT+TGG | 0.265382 | 8.3:-70381898 | MS.gene051125:CDS |
| CATGAGAACAAGAAAGTTAA+AGG | 0.271092 | 8.3:-70381808 | MS.gene051125:CDS |
| TTCCTAAGTCTTTCTTTCCT+TGG | 0.314125 | 8.3:+70381761 | None:intergenic |
| TCTTTCTTTCCTTGGCCCTT+TGG | 0.321490 | 8.3:+70381769 | None:intergenic |
| AAAACACTTGCTTCATCATA+TGG | 0.325245 | 8.3:-70381854 | MS.gene051125:CDS |
| GGTGCTTCTAATGTTTCATT+TGG | 0.377507 | 8.3:+70382042 | None:intergenic |
| TTATCATCTGTGATATCAAT+AGG | 0.408739 | 8.3:+70382021 | None:intergenic |
| CTTATAATGCTGAAAATTCA+AGG | 0.408763 | 8.3:-70381732 | MS.gene051125:CDS |
| GAACTTGAAACAATGGTTGC+AGG | 0.411344 | 8.3:+70381626 | None:intergenic |
| CTTGGTGGAAAATCAACTAA+TGG | 0.416784 | 8.3:-70381880 | MS.gene051125:CDS |
| ACAGAAGCATTGGCAGAAAC+AGG | 0.417435 | 8.3:-70381997 | MS.gene051125:CDS |
| AAGATCTTACATTCATCATC+AGG | 0.417940 | 8.3:+70381192 | None:intergenic |
| TCAGCTTCACTCTTCAAAAT+GGG | 0.432393 | 8.3:+70381064 | None:intergenic |
| GCTTCTAATGTTTCATTTGG+TGG | 0.440855 | 8.3:+70382045 | None:intergenic |
| TAGTCTGGCCAGTAGTAGTT+TGG | 0.463178 | 8.3:+70382106 | None:intergenic |
| TGTTTGATAGTCTTCAATCA+AGG | 0.463299 | 8.3:-70381534 | MS.gene051125:CDS |
| ATGAGAACAAGAAAGTTAAA+GGG | 0.475041 | 8.3:-70381807 | MS.gene051125:CDS |
| TTCTTGATGGTGTTTCTACA+AGG | 0.475367 | 8.3:+70381604 | None:intergenic |
| GGATAATGTTGGTAATTCTC+AGG | 0.478704 | 8.3:-70382157 | MS.gene051125:CDS |
| GAGTGAGACCAGTAGTAGTC+TGG | 0.482515 | 8.3:+70382091 | None:intergenic |
| GCAGACGTCGGAGTAAGGTA+TGG | 0.492817 | 8.3:-70381969 | MS.gene051125:CDS |
| TAAAGTTAATGGAGAAGATA+AGG | 0.493123 | 8.3:-70381929 | MS.gene051125:CDS |
| TGGTGGTGGTTGAGATGATG+AGG | 0.497919 | 8.3:+70382062 | None:intergenic |
| ACATGATCAACAATTGAAAG+TGG | 0.498160 | 8.3:+70381673 | None:intergenic |
| TAACAGCAGACGTCGGAGTA+AGG | 0.498518 | 8.3:-70381974 | MS.gene051125:CDS |
| GGTGGTGGTTGAGATGATGA+GGG | 0.510366 | 8.3:+70382063 | None:intergenic |
| GCAAACATTTCTTGTGCCAA+AGG | 0.510709 | 8.3:-70381785 | MS.gene051125:CDS |
| ACATCAGACATGTGGACCTC+AGG | 0.522328 | 8.3:-70381502 | MS.gene051125:intron |
| TCTAATGTTTCATTTGGTGG+TGG | 0.528703 | 8.3:+70382048 | None:intergenic |
| TATTGCAAGAAACTTCTTGG+TGG | 0.530813 | 8.3:-70381895 | MS.gene051125:CDS |
| CAAACATTTCTTGTGCCAAA+GGG | 0.534415 | 8.3:-70381784 | MS.gene051125:CDS |
| CTACAAGGAACTTGAAACAA+TGG | 0.534720 | 8.3:+70381619 | None:intergenic |
| AGTGCACACCAAACTACTAC+TGG | 0.535841 | 8.3:-70382114 | MS.gene051125:CDS |
| AGATGATAATACAGAAGCAT+TGG | 0.539940 | 8.3:-70382007 | MS.gene051125:CDS |
| GTTTGATAGTCTTCAATCAA+GGG | 0.547183 | 8.3:-70381533 | MS.gene051125:CDS |
| ACTACTGGCCAGACTACTAC+TGG | 0.548115 | 8.3:-70382099 | MS.gene051125:CDS |
| GGCCAAGGAAAGAAAGACTT+AGG | 0.554198 | 8.3:-70381763 | MS.gene051125:CDS |
| TAAAAGTGTATGAGTTTGAG+AGG | 0.558698 | 8.3:-70381573 | MS.gene051125:CDS |
| GGTCCACATGTCTGATGTAA+TGG | 0.568531 | 8.3:+70381507 | None:intergenic |
| TTCATGCATAATTATTGCAG+AGG | 0.572651 | 8.3:+70381699 | None:intergenic |
| AACTTGAAACAATGGTTGCA+GGG | 0.576340 | 8.3:+70381627 | None:intergenic |
| GTGGTGGTTGAGATGATGAG+GGG | 0.582337 | 8.3:+70382064 | None:intergenic |
| CAGACGTCGGAGTAAGGTAT+GGG | 0.586849 | 8.3:-70381968 | MS.gene051125:CDS |
| TTGTAGAAACACCATCAAGA+AGG | 0.587393 | 8.3:-70381602 | MS.gene051125:CDS |
| TTTCTTGTGCCAAAGGGCCA+AGG | 0.594776 | 8.3:-70381778 | MS.gene051125:CDS |
| GAAATGGACATGGATAATGT+TGG | 0.622790 | 8.3:-70382168 | None:intergenic |
| TGGTGGTTGAGATGATGAGG+GGG | 0.630967 | 8.3:+70382065 | None:intergenic |
| ATAGGTGAAATATTGACTAG+TGG | 0.646439 | 8.3:-70381095 | MS.gene051125:intron |
| TGATAGTCTTCAATCAAGGG+TGG | 0.654543 | 8.3:-70381530 | MS.gene051125:CDS |
| TGGCCATTACATCAGACATG+TGG | 0.677334 | 8.3:-70381510 | MS.gene051125:CDS |
| AAACAGGTAACAGCAGACGT+CGG | 0.756698 | 8.3:-70381981 | MS.gene051125:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTGTATTTTTTATTATAT+AGG | - | chr8.3:70382116-70382135 | MS.gene051125:CDS | 10.0% |
| !! | AAACATTAAAGTTAGTAAAT+TGG | + | chr8.3:70382088-70382107 | None:intergenic | 15.0% |
| !! | TATGAATATTATTACTTGTT+AGG | - | chr8.3:70381900-70381919 | MS.gene051125:CDS | 15.0% |
| !! | ATTAAAGTTAGTAAATTGGA+AGG | + | chr8.3:70382084-70382103 | None:intergenic | 20.0% |
| !!! | TTGTTGATCATGTTTATTTT+AGG | - | chr8.3:70381566-70381585 | MS.gene051125:CDS | 20.0% |
| ! | ATGAGAACAAGAAAGTTAAA+GGG | - | chr8.3:70381422-70381441 | MS.gene051125:intron | 25.0% |
| ! | CTTATAATGCTGAAAATTCA+AGG | - | chr8.3:70381497-70381516 | MS.gene051125:intron | 25.0% |
| ! | GGAAGGTAGTATTAAATAAA+TGG | + | chr8.3:70382067-70382086 | None:intergenic | 25.0% |
| ! | TAAAGTTAATGGAGAAGATA+AGG | - | chr8.3:70381300-70381319 | MS.gene051125:intron | 25.0% |
| ! | TTATCATCTGTGATATCAAT+AGG | + | chr8.3:70381211-70381230 | None:intergenic | 25.0% |
| !! | TAATCAAAAAAAGGGGTATA+TGG | - | chr8.3:70381750-70381769 | MS.gene051125:CDS | 25.0% |
| !! | TTTTAATATCTCCTTCTTGA+TGG | + | chr8.3:70381641-70381660 | None:intergenic | 25.0% |
| AAAACACTTGCTTCATCATA+TGG | - | chr8.3:70381375-70381394 | MS.gene051125:intron | 30.0% | |
| AAGATCTTACATTCATCATC+AGG | + | chr8.3:70382040-70382059 | None:intergenic | 30.0% | |
| ACATGATCAACAATTGAAAG+TGG | + | chr8.3:70381559-70381578 | None:intergenic | 30.0% | |
| ACCTCAGGTAATCAAAAAAA+GGG | - | chr8.3:70381742-70381761 | MS.gene051125:CDS | 30.0% | |
| CAAGAAGTTTCTTGCAATAA+TGG | + | chr8.3:70381333-70381352 | None:intergenic | 30.0% | |
| CATGAGAACAAGAAAGTTAA+AGG | - | chr8.3:70381421-70381440 | MS.gene051125:intron | 30.0% | |
| CATTATTGCAAGAAACTTCT+TGG | - | chr8.3:70381331-70381350 | MS.gene051125:intron | 30.0% | |
| GTTACTGCACATTATATTGA+TGG | - | chr8.3:70381775-70381794 | MS.gene051125:CDS | 30.0% | |
| TTCACGAAGCTTAAAGTTAA+TGG | - | chr8.3:70381289-70381308 | MS.gene051125:intron | 30.0% | |
| TTCATGCATAATTATTGCAG+AGG | + | chr8.3:70381533-70381552 | None:intergenic | 30.0% | |
| ! | AGATGATAATACAGAAGCAT+TGG | - | chr8.3:70381222-70381241 | MS.gene051125:CDS | 30.0% |
| ! | ATAGGTGAAATATTGACTAG+TGG | - | chr8.3:70382134-70382153 | MS.gene051125:CDS | 30.0% |
| ! | CACATTATATTGATGGATCT+TGG | - | chr8.3:70381782-70381801 | MS.gene051125:CDS | 30.0% |
| ! | GTTTGATAGTCTTCAATCAA+GGG | - | chr8.3:70381696-70381715 | MS.gene051125:CDS | 30.0% |
| ! | TAAAAGTGTATGAGTTTGAG+AGG | - | chr8.3:70381656-70381675 | MS.gene051125:CDS | 30.0% |
| ! | TGTTTGATAGTCTTCAATCA+AGG | - | chr8.3:70381695-70381714 | MS.gene051125:CDS | 30.0% |
| AACTTGAAACAATGGTTGCA+GGG | + | chr8.3:70381605-70381624 | None:intergenic | 35.0% | |
| CAAACATTTCTTGTGCCAAA+GGG | - | chr8.3:70381445-70381464 | MS.gene051125:intron | 35.0% | |
| CAAAGTCTATCACTTGAATG+AGG | + | chr8.3:70381944-70381963 | None:intergenic | 35.0% | |
| CCTCAGGTAATCAAAAAAAG+GGG | - | chr8.3:70381743-70381762 | MS.gene051125:CDS | 35.0% | |
| CTACAAGGAACTTGAAACAA+TGG | + | chr8.3:70381613-70381632 | None:intergenic | 35.0% | |
| CTTGGTGGAAAATCAACTAA+TGG | - | chr8.3:70381349-70381368 | MS.gene051125:intron | 35.0% | |
| GACCTCAGGTAATCAAAAAA+AGG | - | chr8.3:70381741-70381760 | MS.gene051125:CDS | 35.0% | |
| GCTTCTAATGTTTCATTTGG+TGG | + | chr8.3:70381187-70381206 | None:intergenic | 35.0% | |
| GGTGCTTCTAATGTTTCATT+TGG | + | chr8.3:70381190-70381209 | None:intergenic | 35.0% | |
| TATTGCAAGAAACTTCTTGG+TGG | - | chr8.3:70381334-70381353 | MS.gene051125:intron | 35.0% | |
| TTCCTAAGTCTTTCTTTCCT+TGG | + | chr8.3:70381471-70381490 | None:intergenic | 35.0% | |
| TTGTAGAAACACCATCAAGA+AGG | - | chr8.3:70381627-70381646 | MS.gene051125:CDS | 35.0% | |
| ! | CTTTGTGCTGCTATATTTAC+AGG | - | chr8.3:70381959-70381978 | MS.gene051125:CDS | 35.0% |
| ! | TCTAATGTTTCATTTGGTGG+TGG | + | chr8.3:70381184-70381203 | None:intergenic | 35.0% |
| !! | GGATAATGTTGGTAATTCTC+AGG | - | chr8.3:70381072-70381091 | MS.gene051125:CDS | 35.0% |
| !! | TTCTTGATGGTGTTTCTACA+AGG | + | chr8.3:70381628-70381647 | None:intergenic | 35.0% |
| ATTTACAGAGTCGCATCTTG+AGG | - | chr8.3:70381806-70381825 | MS.gene051125:CDS | 40.0% | |
| GAACTTGAAACAATGGTTGC+AGG | + | chr8.3:70381606-70381625 | None:intergenic | 40.0% | |
| GCAAACATTTCTTGTGCCAA+AGG | - | chr8.3:70381444-70381463 | MS.gene051125:intron | 40.0% | |
| TGATAGTCTTCAATCAAGGG+TGG | - | chr8.3:70381699-70381718 | MS.gene051125:CDS | 40.0% | |
| !!! | CCCCTTTTTTTGATTACCTG+AGG | + | chr8.3:70381746-70381765 | None:intergenic | 40.0% |
| AAACAGGTAACAGCAGACGT+CGG | - | chr8.3:70381248-70381267 | MS.gene051125:CDS | 45.0% | |
| AGTGCACACCAAACTACTAC+TGG | - | chr8.3:70381115-70381134 | MS.gene051125:intron | 45.0% | |
| CTATCACTTGAATGAGGAGC+AGG | + | chr8.3:70381938-70381957 | None:intergenic | 45.0% | |
| GGCCAAGGAAAGAAAGACTT+AGG | - | chr8.3:70381466-70381485 | MS.gene051125:intron | 45.0% | |
| GGTCCACATGTCTGATGTAA+TGG | + | chr8.3:70381725-70381744 | None:intergenic | 45.0% | |
| TAGTCTGGCCAGTAGTAGTT+TGG | + | chr8.3:70381126-70381145 | None:intergenic | 45.0% | |
| TCTTTCTTTCCTTGGCCCTT+TGG | + | chr8.3:70381463-70381482 | None:intergenic | 45.0% | |
| TGGCCATTACATCAGACATG+TGG | - | chr8.3:70381719-70381738 | MS.gene051125:CDS | 45.0% | |
| !! | ACAGAAGCATTGGCAGAAAC+AGG | - | chr8.3:70381232-70381251 | MS.gene051125:CDS | 45.0% |
| ACATCAGACATGTGGACCTC+AGG | - | chr8.3:70381727-70381746 | MS.gene051125:CDS | 50.0% | |
| ACTACTGGCCAGACTACTAC+TGG | - | chr8.3:70381130-70381149 | MS.gene051125:intron | 50.0% | |
| CAGACGTCGGAGTAAGGTAT+GGG | - | chr8.3:70381261-70381280 | MS.gene051125:intron | 50.0% | |
| GAGTGAGACCAGTAGTAGTC+TGG | + | chr8.3:70381141-70381160 | None:intergenic | 50.0% | |
| GTGGTGGTTGAGATGATGAG+GGG | + | chr8.3:70381168-70381187 | None:intergenic | 50.0% | |
| TAACAGCAGACGTCGGAGTA+AGG | - | chr8.3:70381255-70381274 | MS.gene051125:intron | 50.0% | |
| TGGTGGTTGAGATGATGAGG+GGG | + | chr8.3:70381167-70381186 | None:intergenic | 50.0% | |
| TTTCTTGTGCCAAAGGGCCA+AGG | - | chr8.3:70381451-70381470 | MS.gene051125:intron | 50.0% | |
| ! | GGTGGTGGTTGAGATGATGA+GGG | + | chr8.3:70381169-70381188 | None:intergenic | 50.0% |
| !! | TGGTGGTGGTTGAGATGATG+AGG | + | chr8.3:70381170-70381189 | None:intergenic | 50.0% |
| GCAGACGTCGGAGTAAGGTA+TGG | - | chr8.3:70381260-70381279 | MS.gene051125:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 70381070 | 70382181 | 70381070 | ID=MS.gene051125 |
| chr8.3 | mRNA | 70381070 | 70382181 | 70381070 | ID=MS.gene051125.t1;Parent=MS.gene051125 |
| chr8.3 | exon | 70381503 | 70382181 | 70381503 | ID=MS.gene051125.t1.exon1;Parent=MS.gene051125.t1 |
| chr8.3 | CDS | 70381503 | 70382181 | 70381503 | ID=cds.MS.gene051125.t1;Parent=MS.gene051125.t1 |
| chr8.3 | exon | 70381202 | 70381270 | 70381202 | ID=MS.gene051125.t1.exon2;Parent=MS.gene051125.t1 |
| chr8.3 | CDS | 70381202 | 70381270 | 70381202 | ID=cds.MS.gene051125.t1;Parent=MS.gene051125.t1 |
| chr8.3 | exon | 70381070 | 70381113 | 70381070 | ID=MS.gene051125.t1.exon3;Parent=MS.gene051125.t1 |
| chr8.3 | CDS | 70381070 | 70381113 | 70381070 | ID=cds.MS.gene051125.t1;Parent=MS.gene051125.t1 |
| Gene Sequence |
| Protein sequence |
