AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41418


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41418.t1 MTR_1g100697 99.344 305 2 0 1 305 1 305 0.0 619
MS.gene41418.t1 MTR_1g100707 91.475 305 26 0 1 305 1 305 0.0 572
MS.gene41418.t1 MTR_1g100713 55.705 298 128 1 12 305 21 318 2.12e-112 328
MS.gene41418.t1 MTR_0013s0100 53.265 291 134 2 17 305 25 315 1.03e-105 311
MS.gene41418.t1 MTR_4g099030 48.136 295 140 2 12 305 21 303 2.18e-97 289
MS.gene41418.t1 MTR_7g074410 34.812 293 186 2 12 304 13 300 2.06e-53 177
MS.gene41418.t1 MTR_7g074430 32.441 299 197 2 6 304 7 300 9.37e-51 170
MS.gene41418.t1 MTR_7g075970 34.122 296 174 5 17 304 13 295 6.02e-44 152
MS.gene41418.t1 MTR_1g029330 32.313 294 193 4 12 304 13 301 1.09e-40 144
MS.gene41418.t1 MTR_2g018940 29.289 239 143 7 4 233 18 239 8.68e-17 79.7
MS.gene41418.t1 MTR_2g018910 28.151 238 143 7 6 233 23 242 1.98e-16 79.0
MS.gene41418.t1 MTR_8g091730 26.050 238 157 5 3 232 15 241 4.06e-16 77.8
MS.gene41418.t1 MTR_2g018950 27.530 247 145 6 4 233 17 246 1.17e-13 70.9
MS.gene41418.t1 MTR_3g080330 27.193 228 144 5 11 232 13 224 5.30e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41418.t1 AT3G48880 68.562 299 92 2 9 305 11 309 1.34e-152 429
MS.gene41418.t1 AT3G48880 68.562 299 92 2 9 305 11 309 1.34e-152 429
MS.gene41418.t1 AT3G48880 68.562 299 92 2 9 305 11 309 1.34e-152 429
MS.gene41418.t1 AT4G11580 29.532 342 188 11 1 303 4 331 8.73e-33 124

Find 56 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCTCAAGACTCTCAATTT+TGG 0.223803 1.4:-78489946 None:intergenic
ACAAGTATGAGGAAGGATTT+TGG 0.237709 1.4:+78490220 MS.gene41418:CDS
GCAATTTCCTCTAGAAAATA+TGG 0.237803 1.4:-78489841 None:intergenic
CTTGCTGTGATCCACTTCTT+TGG 0.265127 1.4:+78489095 MS.gene41418:CDS
CTTCAGTGAACTCAAGATTA+TGG 0.275898 1.4:+78489879 MS.gene41418:CDS
TCAAGGAAATCTTCAATAAT+CGG 0.286560 1.4:-78489213 None:intergenic
CGCGCAATCGAGAAGCCTTC+TGG 0.341689 1.4:-78490122 None:intergenic
ACGTAAACAAAAGGCTCTAA+TGG 0.360702 1.4:-78489163 None:intergenic
GACATATTCGAGTTAACTTC+TGG 0.361591 1.4:+78489040 MS.gene41418:CDS
TATATCACATTGCATTCTAA+TGG 0.362218 1.4:+78490041 MS.gene41418:CDS
CACTCACGTACAAGTTGAAA+TGG 0.379756 1.4:-78489270 None:intergenic
TTCGGTGTTCAACGCTATAT+AGG 0.386217 1.4:+78489968 MS.gene41418:CDS
CACGAGGAGATGGGAGGATC+TGG 0.389695 1.4:+78488982 MS.gene41418:CDS
GGATCTGGACACAGATATAT+TGG 0.398991 1.4:+78488997 MS.gene41418:CDS
TTCAGTGAACTCAAGATTAT+GGG 0.400982 1.4:+78489880 MS.gene41418:CDS
TGTGCAAGGCAATCCGTTGC+TGG 0.424432 1.4:+78489776 MS.gene41418:CDS
ACTCATTCTGCTCAGATTCA+AGG 0.426417 1.4:-78489230 None:intergenic
AATTGAGAGTCTTGAGCCTT+CGG 0.435748 1.4:+78489950 MS.gene41418:CDS
CAAGGAAATCTTCAATAATC+GGG 0.435917 1.4:-78489212 None:intergenic
GAACTGATGAAGGGATAGTT+AGG 0.442152 1.4:+78490193 MS.gene41418:CDS
AACGACTGGTTCTTCCGGCT+TGG 0.442940 1.4:+78489731 MS.gene41418:CDS
TTGTTTCGAACACGAGGAGA+TGG 0.448455 1.4:+78488972 MS.gene41418:CDS
GCTCAAACGACTGGTTCTTC+CGG 0.448751 1.4:+78489726 MS.gene41418:CDS
AGTTAGGTGGTACAAGTATG+AGG 0.451024 1.4:+78490209 MS.gene41418:CDS
TGGAACAGAATTAAAAGAAC+AGG 0.456792 1.4:+78489751 MS.gene41418:CDS
TTGCTCATGTTTGTAGCGCA+TGG 0.458078 1.4:+78489065 MS.gene41418:CDS
TCGGTGTTCAACGCTATATA+GGG 0.478065 1.4:+78489969 MS.gene41418:CDS
CTTCATCAGTTCTTGTACGT+TGG 0.501934 1.4:-78490182 None:intergenic
CTAAATGTTCTAAGCTGTCT+AGG 0.503752 1.4:-78490008 None:intergenic
CGTTCATCAACGTAAACAAA+AGG 0.517545 1.4:-78489172 None:intergenic
GAACCAGTCGTTTGAGCTGT+GGG 0.524995 1.4:-78489720 None:intergenic
TGTTTCGAACACGAGGAGAT+GGG 0.527745 1.4:+78488973 MS.gene41418:CDS
TGTTTGTAGCGCATGGCGCA+TGG 0.531694 1.4:+78489072 MS.gene41418:CDS
TGATTCTACTATTCGCCAGA+AGG 0.543301 1.4:+78490107 MS.gene41418:CDS
AGGTGGTACAAGTATGAGGA+AGG 0.544931 1.4:+78490213 MS.gene41418:CDS
TATTGTCTGCTTAAAGTGCA+AGG 0.545483 1.4:-78490263 None:intergenic
AATCCGTTGCTGGAAAGAGC+TGG 0.549877 1.4:+78489786 MS.gene41418:CDS
ATTGTCTGCTTAAAGTGCAA+GGG 0.551474 1.4:-78490262 None:intergenic
CAACGTACAAGAACTGATGA+AGG 0.552652 1.4:+78490183 MS.gene41418:CDS
AGAACCAGTCGTTTGAGCTG+TGG 0.570755 1.4:-78489721 None:intergenic
GCGCGAATTTATCACATGCA+TGG 0.572175 1.4:+78490140 MS.gene41418:CDS
ACTCCAGCTCTTTCCAGCAA+CGG 0.573617 1.4:-78489789 None:intergenic
TCAAGCGTCTTCCAAAGAAG+TGG 0.580522 1.4:-78489106 None:intergenic
TTTCCTCTAGAAAATATGGC+GGG 0.587537 1.4:-78489837 None:intergenic
TATGGCGGGTTTGCTATACT+AGG 0.604329 1.4:-78489823 None:intergenic
GTGCCCACAGCTCAAACGAC+TGG 0.606234 1.4:+78489717 MS.gene41418:CDS
TATATAGCGTTGAACACCGA+AGG 0.621770 1.4:-78489966 None:intergenic
TAAAAGAACAGGAATGTGCA+AGG 0.624054 1.4:+78489762 MS.gene41418:CDS
AGTTGACATACACTGCTGAA+AGG 0.630322 1.4:+78489299 MS.gene41418:CDS
AAGACGTTGTTTCGAACACG+AGG 0.632817 1.4:+78488966 MS.gene41418:CDS
TCAGTGAACTCAAGATTATG+GGG 0.652634 1.4:+78489881 MS.gene41418:CDS
TTCGAACACGAGGAGATGGG+AGG 0.657865 1.4:+78488976 MS.gene41418:CDS
AACGTACAAGAACTGATGAA+GGG 0.659917 1.4:+78490184 MS.gene41418:CDS
CTGATGAAGGGATAGTTAGG+TGG 0.665188 1.4:+78490196 MS.gene41418:CDS
ATTTCCTCTAGAAAATATGG+CGG 0.666839 1.4:-78489838 None:intergenic
GAAGCAAAGAAGATGTCACA+TGG 0.687775 1.4:-78489904 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATTTAGAGAGATTAA+TGG + chr1.4:78489337-78489356 MS.gene41418:intron 15.0%
!!! CTCTAAATATATTTTAAAGT+TGG - chr1.4:78489330-78489349 None:intergenic 15.0%
!!! TTATTTTTGAAAAGATAATG+CGG + chr1.4:78489609-78489628 MS.gene41418:intron 15.0%
!!! TTGATAATTTTTTTATGTTG+AGG - chr1.4:78490081-78490100 None:intergenic 15.0%
!!! ATAATTTTTTTATGTTGAGG+AGG - chr1.4:78490078-78490097 None:intergenic 20.0%
!!! TAATTTTTTTATGTTGAGGA+GGG - chr1.4:78490077-78490096 None:intergenic 20.0%
!!! TTTGACGGTTTTTAAATAAA+AGG - chr1.4:78489440-78489459 None:intergenic 20.0%
! TATATCACATTGCATTCTAA+TGG + chr1.4:78490041-78490060 MS.gene41418:CDS 25.0%
! TCAAGGAAATCTTCAATAAT+CGG - chr1.4:78489216-78489235 None:intergenic 25.0%
!!! AATTTTTTTATGTTGAGGAG+GGG - chr1.4:78490076-78490095 None:intergenic 25.0%
!!! TATGCAGTTATTTGTTATGT+TGG - chr1.4:78489413-78489432 None:intergenic 25.0%
CAAGGAAATCTTCAATAATC+GGG - chr1.4:78489215-78489234 None:intergenic 30.0%
CTAGATTGAAGAATTGTGAT+CGG + chr1.4:78489469-78489488 MS.gene41418:intron 30.0%
TGGAACAGAATTAAAAGAAC+AGG + chr1.4:78489751-78489770 MS.gene41418:CDS 30.0%
TTATTAGCACTCCTTCAAAA+TGG + chr1.4:78489645-78489664 MS.gene41418:intron 30.0%
TTCAGTGAACTCAAGATTAT+GGG + chr1.4:78489880-78489899 MS.gene41418:CDS 30.0%
! ATTTCCTCTAGAAAATATGG+CGG - chr1.4:78489841-78489860 None:intergenic 30.0%
! CATTGTACTATCCATTTTGA+AGG - chr1.4:78489659-78489678 None:intergenic 30.0%
! GACGGTTTTTAAATAAAAGG+CGG - chr1.4:78489437-78489456 None:intergenic 30.0%
! GATTCAAGTTATGAGATTGA+TGG - chr1.4:78489542-78489561 None:intergenic 30.0%
! GCAATTTCCTCTAGAAAATA+TGG - chr1.4:78489844-78489863 None:intergenic 30.0%
! TTATGTTGGTGAGTTCTAAT+TGG - chr1.4:78489399-78489418 None:intergenic 30.0%
!!! ATTTTTTTATGTTGAGGAGG+GGG - chr1.4:78490075-78490094 None:intergenic 30.0%
AACGTACAAGAACTGATGAA+GGG + chr1.4:78490184-78490203 MS.gene41418:CDS 35.0%
AGTGTAAAACAACTTTCCAC+TGG - chr1.4:78489371-78489390 None:intergenic 35.0%
ATCTAGCGAATCATGTTTGA+CGG - chr1.4:78489455-78489474 None:intergenic 35.0%
CGTTCATCAACGTAAACAAA+AGG - chr1.4:78489175-78489194 None:intergenic 35.0%
CTTCAGTGAACTCAAGATTA+TGG + chr1.4:78489879-78489898 MS.gene41418:CDS 35.0%
TAAAAGAACAGGAATGTGCA+AGG + chr1.4:78489762-78489781 MS.gene41418:CDS 35.0%
TCAGTGAACTCAAGATTATG+GGG + chr1.4:78489881-78489900 MS.gene41418:CDS 35.0%
! AAGAATTGTGATCGGTTCAT+CGG + chr1.4:78489477-78489496 MS.gene41418:intron 35.0%
! ACAAGTATGAGGAAGGATTT+TGG + chr1.4:78490220-78490239 MS.gene41418:CDS 35.0%
! ACGTAAACAAAAGGCTCTAA+TGG - chr1.4:78489166-78489185 None:intergenic 35.0%
! AGGGGTGAGATTAGTTATTT+AGG - chr1.4:78489689-78489708 None:intergenic 35.0%
! CTAAATGTTCTAAGCTGTCT+AGG - chr1.4:78490011-78490030 None:intergenic 35.0%
! GACATATTCGAGTTAACTTC+TGG + chr1.4:78489040-78489059 MS.gene41418:CDS 35.0%
! TTTCCTCTAGAAAATATGGC+GGG - chr1.4:78489840-78489859 None:intergenic 35.0%
!! AGGATTTTGGAAAGAAGATG+AGG + chr1.4:78490233-78490252 MS.gene41418:CDS 35.0%
!! CTTTTAATTCTGTTCCAAGC+CGG - chr1.4:78489748-78489767 None:intergenic 35.0%
!!! TTTTTTTATGTTGAGGAGGG+GGG - chr1.4:78490074-78490093 None:intergenic 35.0%
AATTGAGAGTCTTGAGCCTT+CGG + chr1.4:78489950-78489969 MS.gene41418:CDS 40.0%
ACTCATTCTGCTCAGATTCA+AGG - chr1.4:78489233-78489252 None:intergenic 40.0%
AGTTAGGTGGTACAAGTATG+AGG + chr1.4:78490209-78490228 MS.gene41418:CDS 40.0%
AGTTGACATACACTGCTGAA+AGG + chr1.4:78489299-78489318 MS.gene41418:CDS 40.0%
CAACGTACAAGAACTGATGA+AGG + chr1.4:78490183-78490202 MS.gene41418:CDS 40.0%
CTACGAAACATCGTACGAAA+AGG - chr1.4:78489571-78489590 None:intergenic 40.0%
CTTCATCAGTTCTTGTACGT+TGG - chr1.4:78490185-78490204 None:intergenic 40.0%
GAACTGATGAAGGGATAGTT+AGG + chr1.4:78490193-78490212 MS.gene41418:CDS 40.0%
GAAGCAAAGAAGATGTCACA+TGG - chr1.4:78489907-78489926 None:intergenic 40.0%
GGATCTGGACACAGATATAT+TGG + chr1.4:78488997-78489016 MS.gene41418:CDS 40.0%
TGATTCTACTATTCGCCAGA+AGG + chr1.4:78490107-78490126 MS.gene41418:CDS 40.0%
! AAACCCGCCATATTTTCTAG+AGG + chr1.4:78489834-78489853 MS.gene41418:CDS 40.0%
! AGGCTCAAGACTCTCAATTT+TGG - chr1.4:78489949-78489968 None:intergenic 40.0%
! CACTCACGTACAAGTTGAAA+TGG - chr1.4:78489273-78489292 None:intergenic 40.0%
! CTTTTCGTACGATGTTTCGT+AGG + chr1.4:78489569-78489588 MS.gene41418:intron 40.0%
! TATATAGCGTTGAACACCGA+AGG - chr1.4:78489969-78489988 None:intergenic 40.0%
! TCGGTGTTCAACGCTATATA+GGG + chr1.4:78489969-78489988 MS.gene41418:CDS 40.0%
!! AGGAGTGCTAATAACTAGCA+AGG - chr1.4:78489639-78489658 None:intergenic 40.0%
!! TTCGGTGTTCAACGCTATAT+AGG + chr1.4:78489968-78489987 MS.gene41418:CDS 40.0%
AGGTGGTACAAGTATGAGGA+AGG + chr1.4:78490213-78490232 MS.gene41418:CDS 45.0%
CGAAACATCGTACGAAAAGG+TGG - chr1.4:78489568-78489587 None:intergenic 45.0%
CTGATGAAGGGATAGTTAGG+TGG + chr1.4:78490196-78490215 MS.gene41418:CDS 45.0%
GCGCGAATTTATCACATGCA+TGG + chr1.4:78490140-78490159 MS.gene41418:CDS 45.0%
TCAAGCGTCTTCCAAAGAAG+TGG - chr1.4:78489109-78489128 None:intergenic 45.0%
TGTTTCGAACACGAGGAGAT+GGG + chr1.4:78488973-78488992 MS.gene41418:CDS 45.0%
TTGCTCATGTTTGTAGCGCA+TGG + chr1.4:78489065-78489084 MS.gene41418:CDS 45.0%
TTGTTTCGAACACGAGGAGA+TGG + chr1.4:78488972-78488991 MS.gene41418:CDS 45.0%
! AAGACGTTGTTTCGAACACG+AGG + chr1.4:78488966-78488985 MS.gene41418:CDS 45.0%
! ATTAATGGATGCACTGCCAG+TGG + chr1.4:78489352-78489371 MS.gene41418:intron 45.0%
! CTTGCTGTGATCCACTTCTT+TGG + chr1.4:78489095-78489114 MS.gene41418:CDS 45.0%
!! TATGGCGGGTTTGCTATACT+AGG - chr1.4:78489826-78489845 None:intergenic 45.0%
AATCCGTTGCTGGAAAGAGC+TGG + chr1.4:78489786-78489805 MS.gene41418:CDS 50.0%
ACTCCAGCTCTTTCCAGCAA+CGG - chr1.4:78489792-78489811 None:intergenic 50.0%
AGAACCAGTCGTTTGAGCTG+TGG - chr1.4:78489724-78489743 None:intergenic 50.0%
CTAATCTCACCCCTTGTTGC+AGG + chr1.4:78489695-78489714 MS.gene41418:intron 50.0%
GAACCAGTCGTTTGAGCTGT+GGG - chr1.4:78489723-78489742 None:intergenic 50.0%
! GCTCAAACGACTGGTTCTTC+CGG + chr1.4:78489726-78489745 MS.gene41418:CDS 50.0%
TGTGCAAGGCAATCCGTTGC+TGG + chr1.4:78489776-78489795 MS.gene41418:CDS 55.0%
TGTTTGTAGCGCATGGCGCA+TGG + chr1.4:78489072-78489091 MS.gene41418:CDS 55.0%
TTCGAACACGAGGAGATGGG+AGG + chr1.4:78488976-78488995 MS.gene41418:CDS 55.0%
! AACGACTGGTTCTTCCGGCT+TGG + chr1.4:78489731-78489750 MS.gene41418:CDS 55.0%
AGCTGTGGGCACCTGCAACA+AGG - chr1.4:78489709-78489728 None:intergenic 60.0%
CACGAGGAGATGGGAGGATC+TGG + chr1.4:78488982-78489001 MS.gene41418:CDS 60.0%
CGCGCAATCGAGAAGCCTTC+TGG - chr1.4:78490125-78490144 None:intergenic 60.0%
CTGTGGGCACCTGCAACAAG+GGG - chr1.4:78489707-78489726 None:intergenic 60.0%
GCTGTGGGCACCTGCAACAA+GGG - chr1.4:78489708-78489727 None:intergenic 60.0%
GTGCCCACAGCTCAAACGAC+TGG + chr1.4:78489717-78489736 MS.gene41418:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 78488962 78490275 78488962 ID=MS.gene41418
chr1.4 mRNA 78488962 78490275 78488962 ID=MS.gene41418.t1;Parent=MS.gene41418
chr1.4 exon 78488962 78489320 78488962 ID=MS.gene41418.t1.exon1;Parent=MS.gene41418.t1
chr1.4 CDS 78488962 78489320 78488962 ID=cds.MS.gene41418.t1;Parent=MS.gene41418.t1
chr1.4 exon 78489717 78490275 78489717 ID=MS.gene41418.t1.exon2;Parent=MS.gene41418.t1
chr1.4 CDS 78489717 78490275 78489717 ID=cds.MS.gene41418.t1;Parent=MS.gene41418.t1
Gene Sequence

>MS.gene41418

ATGGAAGACGTTGTTTCGAACACGAGGAGATGGGAGGATCTGGACACAGATATATTGGTGAAGATTTTTCAGTTGCTTGACATATTCGAGTTAACTTCTGGCATTGCTCATGTTTGTAGCGCATGGCGCATGGCTTGCTGTGATCCACTTCTTTGGAAGACGCTTGATTTGTCAATGTTGAGAAGTAACTTCATCAAGATTCCATTAGAGCCTTTTGTTTACGTTGATGAACGATCTGATAAGAAATTAACCCGATTATTGAAGATTTCCTTGAATCTGAGCAGAATGAGTATAGTGACATTAATCTTCCATTTCAACTTGTACGTGAGTGATGATCAGTTGACATACACTGCTGAAAGGTAATTCCAACTTTAAAATATATTTAGAGAGATTAATGGATGCACTGCCAGTGGAAAGTTGTTTTACACTGACAACCAATTAGAACTCACCAACATAACAAATAACTGCATAACCGCCTTTTATTTAAAAACCGTCAAACATGATTCGCTAGATTGAAGAATTGTGATCGGTTCATCGGATGTCAGTGTAAAATAACTTTACACTTACAATACATATTCCATCAATCTCATAACTTGAATCGAACCACCTTTTCGTACGATGTTTCGTAGGATAAGTTGCTTGTATTTTTATTTTTGAAAAGATAATGCGGCACTCCTTGCTAGTTATTAGCACTCCTTCAAAATGGATAGTACAATGTGAATTGCCTAAATAACTAATCTCACCCCTTGTTGCAGGTGCCCACAGCTCAAACGACTGGTTCTTCCGGCTTGGAACAGAATTAAAAGAACAGGAATGTGCAAGGCAATCCGTTGCTGGAAAGAGCTGGAGTCTCTCACAATGCCTAGTATAGCAAACCCGCCATATTTTCTAGAGGAAATTGCAACAAACTGCAAGAACTTCAGTGAACTCAAGATTATGGGGCCATGTGACATCTTCTTTGCTTCAACTCTTGCTGCATTTGTTCCAAAATTGAGAGTCTTGAGCCTTCGGTGTTCAACGCTATATAGGGATGTTCTCATTCTCATCCTAGACAGCTTAGAACATTTAGAAGTGCTCAATATATCACATTGCATTCTAATGGAATGTCTCCCCCCTCCTCAACATAAAAAAATTATCAATGAAATTGATTCTACTATTCGCCAGAAGGCTTCTCGATTGCGCGAATTTATCACATGCATGGATGACTCGTGTATCATGTGCCAACGTACAAGAACTGATGAAGGGATAGTTAGGTGGTACAAGTATGAGGAAGGATTTTGGAAAGAAGATGAGGTGAAATCCCTTGCACTTTAA

Protein sequence

>MS.gene41418.t1

MEDVVSNTRRWEDLDTDILVKIFQLLDIFELTSGIAHVCSAWRMACCDPLLWKTLDLSMLRSNFIKIPLEPFVYVDERSDKKLTRLLKISLNLSRMSIVTLIFHFNLYVSDDQLTYTAERCPQLKRLVLPAWNRIKRTGMCKAIRCWKELESLTMPSIANPPYFLEEIATNCKNFSELKIMGPCDIFFASTLAAFVPKLRVLSLRCSTLYRDVLILILDSLEHLEVLNISHCILMECLPPPQHKKIINEIDSTIRQKASRLREFITCMDDSCIMCQRTRTDEGIVRWYKYEEGFWKEDEVKSLAL