Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41667.t1 | XP_003602769.1 | 98.7 | 78 | 1 | 0 | 1 | 78 | 1 | 78 | 1.10E-35 | 158.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41667.t1 | Q9XGY4 | 67.1 | 76 | 25 | 0 | 1 | 76 | 1 | 76 | 2.2e-24 | 112.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41667.t1 | G7J3B9 | 98.7 | 78 | 1 | 0 | 1 | 78 | 1 | 78 | 8.0e-36 | 158.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055522 | MS.gene41667 | 0.811905 | 5.54E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41667.t1 | MTR_3g098820 | 98.718 | 78 | 1 | 0 | 1 | 78 | 1 | 78 | 1.20e-54 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41667.t1 | AT5G50810 | 67.105 | 76 | 25 | 0 | 1 | 76 | 1 | 76 | 2.86e-30 | 102 |
Find 17 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGCCATGATTAATGAAA+TGG | 0.403854 | 3.4:+88751531 | MS.gene41667:CDS |
| TGCCACCATTTCATTAATCA+TGG | 0.406262 | 3.4:-88751536 | None:intergenic |
| AAATGCATCACAGGCACGCC+TGG | 0.412006 | 3.4:+88751583 | MS.gene41667:CDS |
| CCTGGGAATAAGTTCAGTTC+CGG | 0.455891 | 3.4:+88751601 | MS.gene41667:CDS |
| CAAAGATGACTAGTCAATGC+TGG | 0.492799 | 3.4:+88751557 | MS.gene41667:CDS |
| TCTGCATTTCAGCAGAATTG+AGG | 0.501147 | 3.4:-88750607 | None:intergenic |
| ACACTGTGCACAGCGTTACA+TGG | 0.508169 | 3.4:+88751642 | MS.gene41667:CDS |
| AACGCTGTGCACAGTGTGTT+AGG | 0.520352 | 3.4:-88751636 | None:intergenic |
| TGCTGGGACAAATGCATCAC+AGG | 0.525844 | 3.4:+88751574 | MS.gene41667:CDS |
| TGAGCATGCTTATCATGAAA+CGG | 0.531078 | 3.4:+88751668 | MS.gene41667:CDS |
| CCGGAACTGAACTTATTCCC+AGG | 0.566395 | 3.4:-88751601 | None:intergenic |
| AAAGATGACTAGTCAATGCT+GGG | 0.584821 | 3.4:+88751558 | MS.gene41667:CDS |
| AATGCATCACAGGCACGCCT+GGG | 0.596603 | 3.4:+88751584 | MS.gene41667:CDS |
| TGTTAGGCAATTAGTCTCAC+CGG | 0.596955 | 3.4:-88751620 | None:intergenic |
| TCAATTCTGCTGAAATGCAG+AGG | 0.611780 | 3.4:+88750609 | MS.gene41667:CDS |
| AGCCATGATTAATGAAATGG+TGG | 0.612893 | 3.4:+88751534 | MS.gene41667:CDS |
| CATTTCAGCAGAATTGAGGT+CGG | 0.629707 | 3.4:-88750603 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGATATTTAAAAGTGATT+TGG | + | chr3.4:88751002-88751021 | MS.gene41667:intron | 15.0% |
| !!! | ATTTTTTTCCATAAGATAAA+GGG | - | chr3.4:88751123-88751142 | None:intergenic | 15.0% |
| !!! | TATTTTTTTCCATAAGATAA+AGG | - | chr3.4:88751124-88751143 | None:intergenic | 15.0% |
| !! | AAATGTAATCGAATTGAAAA+GGG | - | chr3.4:88750686-88750705 | None:intergenic | 20.0% |
| !! | ATTATCATTATTATGGCTTA+GGG | + | chr3.4:88751351-88751370 | MS.gene41667:intron | 20.0% |
| !! | TCTAATAAAAGTTGTAACTA+TGG | + | chr3.4:88751172-88751191 | MS.gene41667:intron | 20.0% |
| !!! | TTTTTTTCCATAAGATAAAG+GGG | - | chr3.4:88751122-88751141 | None:intergenic | 20.0% |
| ! | AAAAGTTGTAACTATGGAAT+TGG | + | chr3.4:88751178-88751197 | MS.gene41667:intron | 25.0% |
| ! | AATGTAATCGAATTGAAAAG+GGG | - | chr3.4:88750685-88750704 | None:intergenic | 25.0% |
| ! | AGATTGAAATACCATATAAG+TGG | - | chr3.4:88751432-88751451 | None:intergenic | 25.0% |
| ! | CAAATGTAATCGAATTGAAA+AGG | - | chr3.4:88750687-88750706 | None:intergenic | 25.0% |
| ! | CACATATAAGTGGTATATAT+CGG | - | chr3.4:88751392-88751411 | None:intergenic | 25.0% |
| ! | CATTATCATTATTATGGCTT+AGG | + | chr3.4:88751350-88751369 | MS.gene41667:intron | 25.0% |
| ! | CTAAGCCATAATAATGATAA+TGG | - | chr3.4:88751352-88751371 | None:intergenic | 25.0% |
| ! | GGTTGTTATATTTCATTACA+GGG | - | chr3.4:88751477-88751496 | None:intergenic | 25.0% |
| ! | GTTGTTATATTTCATTACAG+GGG | - | chr3.4:88751476-88751495 | None:intergenic | 25.0% |
| ! | TTATCATTATTATGGCTTAG+GGG | + | chr3.4:88751352-88751371 | MS.gene41667:intron | 25.0% |
| AAGAGCCATGATTAATGAAA+TGG | + | chr3.4:88751531-88751550 | MS.gene41667:CDS | 30.0% | |
| ACCATATAAGTGGCATATAT+CGG | - | chr3.4:88751422-88751441 | None:intergenic | 30.0% | |
| AGTATCTGTTAATCGTAAGA+AGG | - | chr3.4:88750662-88750681 | None:intergenic | 30.0% | |
| CACTGCCATTATCATTATTA+TGG | + | chr3.4:88751344-88751363 | MS.gene41667:intron | 30.0% | |
| CGATATATACCACTTATATG+TGG | + | chr3.4:88751390-88751409 | MS.gene41667:intron | 30.0% | |
| GGGTTGTTATATTTCATTAC+AGG | - | chr3.4:88751478-88751497 | None:intergenic | 30.0% | |
| TAACAGTTATCACTAAGACA+AGG | - | chr3.4:88750821-88750840 | None:intergenic | 30.0% | |
| ! | AAGGTTGATTCTGTTACTAT+AGG | + | chr3.4:88750898-88750917 | MS.gene41667:intron | 30.0% |
| ! | TATCAATTTTATGGTGGCTA+AGG | + | chr3.4:88750879-88750898 | MS.gene41667:intron | 30.0% |
| !! | AGATTTGATTTTTCATGCTG+AGG | + | chr3.4:88750855-88750874 | MS.gene41667:intron | 30.0% |
| !! | ATAGGTTTTTAAGAGAGACA+TGG | - | chr3.4:88751252-88751271 | None:intergenic | 30.0% |
| !! | CATAATAATGATAATGGCAG+TGG | - | chr3.4:88751346-88751365 | None:intergenic | 30.0% |
| AAGAAGACCCCTTTATCTTA+TGG | + | chr3.4:88751112-88751131 | MS.gene41667:intron | 35.0% | |
| ACAAAGCTACAGTAGACTAT+AGG | - | chr3.4:88751270-88751289 | None:intergenic | 35.0% | |
| AGCCATGATTAATGAAATGG+TGG | + | chr3.4:88751534-88751553 | MS.gene41667:CDS | 35.0% | |
| ATACTGTTCTGTTAGTGCTA+AGG | + | chr3.4:88750942-88750961 | MS.gene41667:intron | 35.0% | |
| TGAGCATGCTTATCATGAAA+CGG | + | chr3.4:88751668-88751687 | MS.gene41667:CDS | 35.0% | |
| TGCCACCATTTCATTAATCA+TGG | - | chr3.4:88751539-88751558 | None:intergenic | 35.0% | |
| ! | AAAGATGACTAGTCAATGCT+GGG | + | chr3.4:88751558-88751577 | MS.gene41667:CDS | 35.0% |
| ! | AGATAAAGGGGTCTTCTTTT+TGG | - | chr3.4:88751110-88751129 | None:intergenic | 35.0% |
| ! | TCCGATATATGCCACTTATA+TGG | + | chr3.4:88751418-88751437 | MS.gene41667:intron | 35.0% |
| ! | TGAGGCTATCAATTTTATGG+TGG | + | chr3.4:88750873-88750892 | MS.gene41667:intron | 35.0% |
| ! | TGCTGAGGCTATCAATTTTA+TGG | + | chr3.4:88750870-88750889 | MS.gene41667:intron | 35.0% |
| !! | TTTGTTGTTCTTCCTGCATT+TGG | - | chr3.4:88751513-88751532 | None:intergenic | 35.0% |
| AATCGACCAAGTCTAACCAA+CGG | - | chr3.4:88751087-88751106 | None:intergenic | 40.0% | |
| ATGGAATTGGTACCCAAAGA+CGG | + | chr3.4:88751191-88751210 | MS.gene41667:intron | 40.0% | |
| ATGTTTGACTGCATTTCCGT+TGG | + | chr3.4:88751068-88751087 | MS.gene41667:intron | 40.0% | |
| ATTATCTTCCCTCCGTCTTT+GGG | - | chr3.4:88751206-88751225 | None:intergenic | 40.0% | |
| CATTTCAGCAGAATTGAGGT+CGG | - | chr3.4:88750606-88750625 | None:intergenic | 40.0% | |
| CGGAATATGCCACATATAAG+TGG | - | chr3.4:88751402-88751421 | None:intergenic | 40.0% | |
| GCATTTGGAGGGCAAAATTA+GGG | - | chr3.4:88751498-88751517 | None:intergenic | 40.0% | |
| TATGGCTTAGGGGATCTAAA+TGG | + | chr3.4:88751362-88751381 | MS.gene41667:intron | 40.0% | |
| TATTATCTTCCCTCCGTCTT+TGG | - | chr3.4:88751207-88751226 | None:intergenic | 40.0% | |
| TCAATTCTGCTGAAATGCAG+AGG | + | chr3.4:88750609-88750628 | MS.gene41667:CDS | 40.0% | |
| TCTGCATTTCAGCAGAATTG+AGG | - | chr3.4:88750610-88750629 | None:intergenic | 40.0% | |
| TGCATTTGGAGGGCAAAATT+AGG | - | chr3.4:88751499-88751518 | None:intergenic | 40.0% | |
| TTGTTCTTCCTGCATTTGGA+GGG | - | chr3.4:88751509-88751528 | None:intergenic | 40.0% | |
| TTTCATTACAGGGGAACAAC+AGG | - | chr3.4:88751467-88751486 | None:intergenic | 40.0% | |
| ! | CAAAGATGACTAGTCAATGC+TGG | + | chr3.4:88751557-88751576 | MS.gene41667:CDS | 40.0% |
| ! | TAATTTTGCCCTCCAAATGC+AGG | + | chr3.4:88751498-88751517 | MS.gene41667:intron | 40.0% |
| ! | TGTTAGGCAATTAGTCTCAC+CGG | - | chr3.4:88751623-88751642 | None:intergenic | 40.0% |
| AAGAGAGACATGGCAAACAG+TGG | - | chr3.4:88751242-88751261 | None:intergenic | 45.0% | |
| AATTGGTACCCAAAGACGGA+GGG | + | chr3.4:88751195-88751214 | MS.gene41667:intron | 45.0% | |
| CCTGGGAATAAGTTCAGTTC+CGG | + | chr3.4:88751601-88751620 | MS.gene41667:CDS | 45.0% | |
| GTTGTTCTTCCTGCATTTGG+AGG | - | chr3.4:88751510-88751529 | None:intergenic | 45.0% | |
| ! | ATTTGGTTCACTTCAGCTGC+TGG | + | chr3.4:88751019-88751038 | MS.gene41667:intron | 45.0% |
| ! | GCATTTCCGTTGGTTAGACT+TGG | + | chr3.4:88751078-88751097 | MS.gene41667:intron | 45.0% |
| !!! | AAGGCCAGTTGGTTTTTGGA+GGG | + | chr3.4:88750961-88750980 | MS.gene41667:intron | 45.0% |
| !!! | TAAGGCCAGTTGGTTTTTGG+AGG | + | chr3.4:88750960-88750979 | MS.gene41667:intron | 45.0% |
| !!! | TGCTAAGGCCAGTTGGTTTT+TGG | + | chr3.4:88750957-88750976 | MS.gene41667:intron | 45.0% |
| AACGCTGTGCACAGTGTGTT+AGG | - | chr3.4:88751639-88751658 | None:intergenic | 50.0% | |
| ACACTGTGCACAGCGTTACA+TGG | + | chr3.4:88751642-88751661 | MS.gene41667:CDS | 50.0% | |
| CCGGAACTGAACTTATTCCC+AGG | - | chr3.4:88751604-88751623 | None:intergenic | 50.0% | |
| CTGTTAGTGCTAAGGCCAGT+TGG | + | chr3.4:88750950-88750969 | MS.gene41667:intron | 50.0% | |
| GAATTGGTACCCAAAGACGG+AGG | + | chr3.4:88751194-88751213 | MS.gene41667:intron | 50.0% | |
| TGCTGGGACAAATGCATCAC+AGG | + | chr3.4:88751574-88751593 | MS.gene41667:CDS | 50.0% | |
| !!! | AGGCCAGTTGGTTTTTGGAG+GGG | + | chr3.4:88750962-88750981 | MS.gene41667:intron | 50.0% |
| !!! | CAGTTGGTTTTTGGAGGGGA+CGG | + | chr3.4:88750966-88750985 | MS.gene41667:intron | 50.0% |
| AAATGCATCACAGGCACGCC+TGG | + | chr3.4:88751583-88751602 | MS.gene41667:CDS | 55.0% | |
| AATGCATCACAGGCACGCCT+GGG | + | chr3.4:88751584-88751603 | MS.gene41667:CDS | 55.0% | |
| CGTCCCCTCCAAAAACCAAC+TGG | - | chr3.4:88750968-88750987 | None:intergenic | 55.0% | |
| !! | GTTTTTGGAGGGGACGGCTA+AGG | + | chr3.4:88750972-88750991 | MS.gene41667:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 88750593 | 88751708 | 88750593 | ID=MS.gene41667 |
| chr3.4 | mRNA | 88750593 | 88751708 | 88750593 | ID=MS.gene41667.t1;Parent=MS.gene41667 |
| chr3.4 | exon | 88750593 | 88750640 | 88750593 | ID=MS.gene41667.t1.exon1;Parent=MS.gene41667.t1 |
| chr3.4 | CDS | 88750593 | 88750640 | 88750593 | ID=cds.MS.gene41667.t1;Parent=MS.gene41667.t1 |
| chr3.4 | exon | 88751520 | 88751708 | 88751520 | ID=MS.gene41667.t1.exon2;Parent=MS.gene41667.t1 |
| chr3.4 | CDS | 88751520 | 88751708 | 88751520 | ID=cds.MS.gene41667.t1;Parent=MS.gene41667.t1 |
| Gene Sequence |
| Protein sequence |
