Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055522.t1 | XP_003602769.1 | 98.7 | 78 | 1 | 0 | 1 | 78 | 1 | 78 | 1.10E-35 | 158.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055522.t1 | Q9XGY4 | 67.1 | 76 | 25 | 0 | 1 | 76 | 1 | 76 | 2.2e-24 | 112.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055522.t1 | G7J3B9 | 98.7 | 78 | 1 | 0 | 1 | 78 | 1 | 78 | 8.0e-36 | 158.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055522 | MS.gene41667 | 0.811905 | 5.54E-51 | -1.69E-46 |
| MS.gene055522 | MS.gene95848 | 0.844347 | 8.00E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055522.t1 | MTR_3g098820 | 98.718 | 78 | 1 | 0 | 1 | 78 | 1 | 78 | 1.20e-54 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055522.t1 | AT5G50810 | 67.105 | 76 | 25 | 0 | 1 | 76 | 1 | 76 | 2.86e-30 | 102 |
Find 17 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGCCATGATTAATGAAA+TGG | 0.403854 | 3.2:+82242203 | MS.gene055522:CDS |
| TGCCACCATTTCATTAATCA+TGG | 0.406262 | 3.2:-82242208 | None:intergenic |
| AAATGCATCACAGGCACGCC+TGG | 0.412006 | 3.2:+82242255 | MS.gene055522:CDS |
| CCTGGGAATAAGTTCAGTTC+CGG | 0.455891 | 3.2:+82242273 | MS.gene055522:CDS |
| CAAAGATGACTAGTCAATGC+TGG | 0.492799 | 3.2:+82242229 | MS.gene055522:CDS |
| TCTGCATTTCAGCAGAATTG+AGG | 0.501147 | 3.2:-82241303 | None:intergenic |
| ACACTGTGCACAGCGTTACA+TGG | 0.508169 | 3.2:+82242314 | MS.gene055522:CDS |
| AACGCTGTGCACAGTGTGTT+AGG | 0.520352 | 3.2:-82242308 | None:intergenic |
| TGCTGGGACAAATGCATCAC+AGG | 0.525844 | 3.2:+82242246 | MS.gene055522:CDS |
| TGAGCATGCTTATCATGAAA+CGG | 0.531078 | 3.2:+82242340 | MS.gene055522:CDS |
| CCGGAACTGAACTTATTCCC+AGG | 0.566395 | 3.2:-82242273 | None:intergenic |
| AAAGATGACTAGTCAATGCT+GGG | 0.584821 | 3.2:+82242230 | MS.gene055522:CDS |
| AATGCATCACAGGCACGCCT+GGG | 0.596603 | 3.2:+82242256 | MS.gene055522:CDS |
| TGTTAGGCAATTAGTCTCAC+CGG | 0.596955 | 3.2:-82242292 | None:intergenic |
| TCAATTCTGCTGAAATGCAG+AGG | 0.611780 | 3.2:+82241305 | MS.gene055522:CDS |
| AGCCATGATTAATGAAATGG+TGG | 0.612893 | 3.2:+82242206 | MS.gene055522:CDS |
| CATTTCAGCAGAATTGAGGT+CGG | 0.629707 | 3.2:-82241299 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGATATTTAAAAGTGATT+TGG | + | chr3.2:82241704-82241723 | MS.gene055522:intron | 15.0% |
| !!! | ATTTTTTTCCATAAGATAAA+GGG | - | chr3.2:82241825-82241844 | None:intergenic | 15.0% |
| !!! | TATTTTTTTCCATAAGATAA+AGG | - | chr3.2:82241826-82241845 | None:intergenic | 15.0% |
| !! | ATTATCATTATTATGGCTTA+GGG | + | chr3.2:82242053-82242072 | MS.gene055522:intron | 20.0% |
| !! | TCTAATAATAGTTGTAACTA+TGG | + | chr3.2:82241874-82241893 | MS.gene055522:intron | 20.0% |
| !!! | TTTTTTTCCATAAGATAAAG+GGG | - | chr3.2:82241824-82241843 | None:intergenic | 20.0% |
| ! | AATAGTTGTAACTATGGAAT+TGG | + | chr3.2:82241880-82241899 | MS.gene055522:intron | 25.0% |
| ! | AATCGAATCGAATTGAAAAA+TGG | - | chr3.2:82241381-82241400 | None:intergenic | 25.0% |
| ! | AGATTGAAATACCATATAAG+TGG | - | chr3.2:82242104-82242123 | None:intergenic | 25.0% |
| ! | CATTATCATTATTATGGCTT+AGG | + | chr3.2:82242052-82242071 | MS.gene055522:intron | 25.0% |
| ! | CTAAGCCATAATAATGATAA+TGG | - | chr3.2:82242054-82242073 | None:intergenic | 25.0% |
| ! | GGTTGTTATATTTCATTACA+GGG | - | chr3.2:82242149-82242168 | None:intergenic | 25.0% |
| ! | GTTGTTATATTTCATTACAG+GGG | - | chr3.2:82242148-82242167 | None:intergenic | 25.0% |
| ! | TTATCATTATTATGGCTTAG+GGG | + | chr3.2:82242054-82242073 | MS.gene055522:intron | 25.0% |
| AAGAGCCATGATTAATGAAA+TGG | + | chr3.2:82242203-82242222 | MS.gene055522:CDS | 30.0% | |
| ACCATATAAGTGGCATATAT+CGG | - | chr3.2:82242094-82242113 | None:intergenic | 30.0% | |
| AGTATCTGTTAATCGTAAGA+AGG | - | chr3.2:82241358-82241377 | None:intergenic | 30.0% | |
| CACTGCCATTATCATTATTA+TGG | + | chr3.2:82242046-82242065 | MS.gene055522:intron | 30.0% | |
| GGGTTGTTATATTTCATTAC+AGG | - | chr3.2:82242150-82242169 | None:intergenic | 30.0% | |
| TAACAGTTATCACTAAGACA+AGG | - | chr3.2:82241523-82241542 | None:intergenic | 30.0% | |
| ! | TATCAATTTTATGGTGGCTA+AGG | + | chr3.2:82241581-82241600 | MS.gene055522:intron | 30.0% |
| !! | AGATTTGATTTTTCATGCTG+AGG | + | chr3.2:82241557-82241576 | MS.gene055522:intron | 30.0% |
| !! | ATAGGTTTTTAAGAGAGACA+TGG | - | chr3.2:82241954-82241973 | None:intergenic | 30.0% |
| !! | CATAATAATGATAATGGCAG+TGG | - | chr3.2:82242048-82242067 | None:intergenic | 30.0% |
| AAGAAGACCCCTTTATCTTA+TGG | + | chr3.2:82241814-82241833 | MS.gene055522:intron | 35.0% | |
| ACAAAGCTACAGTAGACTAT+AGG | - | chr3.2:82241972-82241991 | None:intergenic | 35.0% | |
| AGCCATGATTAATGAAATGG+TGG | + | chr3.2:82242206-82242225 | MS.gene055522:CDS | 35.0% | |
| ATACTGTTCTGTTAGTGCTA+AGG | + | chr3.2:82241644-82241663 | MS.gene055522:intron | 35.0% | |
| ATGTTTGACTACATTTCCGT+TGG | + | chr3.2:82241770-82241789 | MS.gene055522:intron | 35.0% | |
| TGAGCATGCTTATCATGAAA+CGG | + | chr3.2:82242340-82242359 | MS.gene055522:CDS | 35.0% | |
| TGCCACCATTTCATTAATCA+TGG | - | chr3.2:82242211-82242230 | None:intergenic | 35.0% | |
| ! | AAAGATGACTAGTCAATGCT+GGG | + | chr3.2:82242230-82242249 | MS.gene055522:CDS | 35.0% |
| ! | AGATAAAGGGGTCTTCTTTT+TGG | - | chr3.2:82241812-82241831 | None:intergenic | 35.0% |
| ! | TCCGATATATGCCACTTATA+TGG | + | chr3.2:82242090-82242109 | MS.gene055522:intron | 35.0% |
| ! | TGAGGCTATCAATTTTATGG+TGG | + | chr3.2:82241575-82241594 | MS.gene055522:intron | 35.0% |
| ! | TGCTGAGGCTATCAATTTTA+TGG | + | chr3.2:82241572-82241591 | MS.gene055522:intron | 35.0% |
| !! | AAGGTTGATTCGGTTACTAT+AGG | + | chr3.2:82241600-82241619 | MS.gene055522:intron | 35.0% |
| !! | TTTGTTGTTCTTCCTGCATT+TGG | - | chr3.2:82242185-82242204 | None:intergenic | 35.0% |
| AATCGACCAAGTCTAACCAA+CGG | - | chr3.2:82241789-82241808 | None:intergenic | 40.0% | |
| ATGGAATTGGTACCCAAACA+CGG | + | chr3.2:82241893-82241912 | MS.gene055522:intron | 40.0% | |
| ATTATCTTCCCTCCGTGTTT+GGG | - | chr3.2:82241908-82241927 | None:intergenic | 40.0% | |
| CATTTCAGCAGAATTGAGGT+CGG | - | chr3.2:82241302-82241321 | None:intergenic | 40.0% | |
| GCATTTGGAGGGCAAAATTA+GGG | - | chr3.2:82242170-82242189 | None:intergenic | 40.0% | |
| TATGGCTTAGGGGATCTAAA+TGG | + | chr3.2:82242064-82242083 | MS.gene055522:intron | 40.0% | |
| TATTATCTTCCCTCCGTGTT+TGG | - | chr3.2:82241909-82241928 | None:intergenic | 40.0% | |
| TCAATTCTGCTGAAATGCAG+AGG | + | chr3.2:82241305-82241324 | MS.gene055522:CDS | 40.0% | |
| TCTGCATTTCAGCAGAATTG+AGG | - | chr3.2:82241306-82241325 | None:intergenic | 40.0% | |
| TGCATTTGGAGGGCAAAATT+AGG | - | chr3.2:82242171-82242190 | None:intergenic | 40.0% | |
| TTGTTCTTCCTGCATTTGGA+GGG | - | chr3.2:82242181-82242200 | None:intergenic | 40.0% | |
| TTTCATTACAGGGGAACAAC+AGG | - | chr3.2:82242139-82242158 | None:intergenic | 40.0% | |
| ! | ACATTTCCGTTGGTTAGACT+TGG | + | chr3.2:82241780-82241799 | MS.gene055522:intron | 40.0% |
| ! | CAAAGATGACTAGTCAATGC+TGG | + | chr3.2:82242229-82242248 | MS.gene055522:CDS | 40.0% |
| ! | TAATTTTGCCCTCCAAATGC+AGG | + | chr3.2:82242170-82242189 | MS.gene055522:intron | 40.0% |
| ! | TATGGTGGCTAAGGTTGATT+CGG | + | chr3.2:82241590-82241609 | MS.gene055522:intron | 40.0% |
| ! | TGTTAGGCAATTAGTCTCAC+CGG | - | chr3.2:82242295-82242314 | None:intergenic | 40.0% |
| AAGAGAGACATGGCAAACAG+TGG | - | chr3.2:82241944-82241963 | None:intergenic | 45.0% | |
| AATTGGTACCCAAACACGGA+GGG | + | chr3.2:82241897-82241916 | MS.gene055522:intron | 45.0% | |
| CCTGGGAATAAGTTCAGTTC+CGG | + | chr3.2:82242273-82242292 | MS.gene055522:CDS | 45.0% | |
| GTTGTTCTTCCTGCATTTGG+AGG | - | chr3.2:82242182-82242201 | None:intergenic | 45.0% | |
| ! | ATTTGGTTCACTTCAGCTGC+TGG | + | chr3.2:82241721-82241740 | MS.gene055522:intron | 45.0% |
| !!! | AAGGCCAGTTGGTTTTTGGA+GGG | + | chr3.2:82241663-82241682 | MS.gene055522:intron | 45.0% |
| !!! | TAAGGCCAGTTGGTTTTTGG+AGG | + | chr3.2:82241662-82241681 | MS.gene055522:intron | 45.0% |
| !!! | TGCTAAGGCCAGTTGGTTTT+TGG | + | chr3.2:82241659-82241678 | MS.gene055522:intron | 45.0% |
| AACGCTGTGCACAGTGTGTT+AGG | - | chr3.2:82242311-82242330 | None:intergenic | 50.0% | |
| ACACTGTGCACAGCGTTACA+TGG | + | chr3.2:82242314-82242333 | MS.gene055522:CDS | 50.0% | |
| CCGGAACTGAACTTATTCCC+AGG | - | chr3.2:82242276-82242295 | None:intergenic | 50.0% | |
| CTGTTAGTGCTAAGGCCAGT+TGG | + | chr3.2:82241652-82241671 | MS.gene055522:intron | 50.0% | |
| GAATTGGTACCCAAACACGG+AGG | + | chr3.2:82241896-82241915 | MS.gene055522:intron | 50.0% | |
| TGCTGGGACAAATGCATCAC+AGG | + | chr3.2:82242246-82242265 | MS.gene055522:CDS | 50.0% | |
| !!! | AGGCCAGTTGGTTTTTGGAG+GGG | + | chr3.2:82241664-82241683 | MS.gene055522:intron | 50.0% |
| !!! | CAGTTGGTTTTTGGAGGGGA+CGG | + | chr3.2:82241668-82241687 | MS.gene055522:intron | 50.0% |
| AAATGCATCACAGGCACGCC+TGG | + | chr3.2:82242255-82242274 | MS.gene055522:CDS | 55.0% | |
| AATGCATCACAGGCACGCCT+GGG | + | chr3.2:82242256-82242275 | MS.gene055522:CDS | 55.0% | |
| CGTCCCCTCCAAAAACCAAC+TGG | - | chr3.2:82241670-82241689 | None:intergenic | 55.0% | |
| !! | GTTTTTGGAGGGGACGGCTA+AGG | + | chr3.2:82241674-82241693 | MS.gene055522:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 82241289 | 82242380 | 82241289 | ID=MS.gene055522 |
| chr3.2 | mRNA | 82241289 | 82242380 | 82241289 | ID=MS.gene055522.t1;Parent=MS.gene055522 |
| chr3.2 | exon | 82241289 | 82241336 | 82241289 | ID=MS.gene055522.t1.exon1;Parent=MS.gene055522.t1 |
| chr3.2 | CDS | 82241289 | 82241336 | 82241289 | ID=cds.MS.gene055522.t1;Parent=MS.gene055522.t1 |
| chr3.2 | exon | 82242192 | 82242380 | 82242192 | ID=MS.gene055522.t1.exon2;Parent=MS.gene055522.t1 |
| chr3.2 | CDS | 82242192 | 82242380 | 82242192 | ID=cds.MS.gene055522.t1;Parent=MS.gene055522.t1 |
| Gene Sequence |
| Protein sequence |
