Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41686.t1 | AEO16612.1 | 100 | 101 | 0 | 0 | 1 | 101 | 1 | 101 | 1.90E-51 | 211.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41686.t1 | Q93WI9 | 68.0 | 100 | 32 | 0 | 2 | 101 | 4 | 103 | 8.5e-37 | 154.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41686.t1 | G3FCS8 | 100.0 | 101 | 0 | 0 | 1 | 101 | 1 | 101 | 1.3e-51 | 211.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene41686 | 0.815129 | 1.08E-51 | -1.69E-46 |
| MS.gene049552 | MS.gene41686 | 0.802199 | 6.28E-49 | -1.69E-46 |
| MS.gene056046 | MS.gene41686 | 0.807502 | 4.90E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene41686 | 0.800529 | 1.38E-48 | -1.69E-46 |
| MS.gene059367 | MS.gene41686 | 0.83343 | 5.36E-56 | -1.69E-46 |
| MS.gene060778 | MS.gene41686 | 0.848212 | 7.08E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41686.t1 | MTR_7g084970 | 98.020 | 101 | 2 | 0 | 1 | 101 | 1 | 101 | 2.59e-69 | 205 |
| MS.gene41686.t1 | MTR_6g033040 | 73.267 | 101 | 27 | 0 | 1 | 101 | 1 | 101 | 2.08e-51 | 159 |
| MS.gene41686.t1 | MTR_7g085020 | 69.608 | 102 | 30 | 1 | 1 | 101 | 1 | 102 | 3.67e-46 | 146 |
| MS.gene41686.t1 | MTR_7g006630 | 69.792 | 96 | 28 | 1 | 7 | 101 | 2 | 97 | 1.52e-40 | 132 |
| MS.gene41686.t1 | MTR_7g006690 | 65.686 | 102 | 34 | 1 | 1 | 101 | 1 | 102 | 3.71e-40 | 131 |
| MS.gene41686.t1 | MTR_0020s0120 | 60.638 | 94 | 37 | 0 | 8 | 101 | 7 | 100 | 1.39e-39 | 129 |
| MS.gene41686.t1 | MTR_7g104460 | 56.436 | 101 | 44 | 0 | 1 | 101 | 1 | 101 | 2.09e-38 | 126 |
| MS.gene41686.t1 | MTR_2g086270 | 56.842 | 95 | 41 | 0 | 7 | 101 | 6 | 100 | 1.06e-37 | 124 |
| MS.gene41686.t1 | MTR_1g060190 | 54.455 | 101 | 46 | 0 | 1 | 101 | 1 | 101 | 2.01e-37 | 124 |
| MS.gene41686.t1 | MTR_0020s0120 | 57.447 | 94 | 40 | 0 | 8 | 101 | 7 | 100 | 1.23e-36 | 122 |
| MS.gene41686.t1 | MTR_7g085040 | 60.227 | 88 | 35 | 0 | 14 | 101 | 11 | 98 | 3.96e-36 | 120 |
| MS.gene41686.t1 | MTR_8g106840 | 55.670 | 97 | 42 | 1 | 1 | 97 | 1 | 96 | 1.93e-33 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41686.t1 | AT1G65480 | 65.347 | 101 | 35 | 0 | 1 | 101 | 1 | 101 | 5.39e-45 | 143 |
| MS.gene41686.t1 | AT1G65480 | 65.347 | 101 | 35 | 0 | 1 | 101 | 45 | 145 | 8.03e-45 | 144 |
| MS.gene41686.t1 | AT4G20370 | 61.386 | 101 | 39 | 0 | 1 | 101 | 1 | 101 | 4.64e-41 | 133 |
| MS.gene41686.t1 | AT5G62040 | 59.375 | 96 | 38 | 1 | 7 | 101 | 6 | 101 | 2.71e-38 | 126 |
| MS.gene41686.t1 | AT5G03840 | 54.737 | 95 | 43 | 0 | 7 | 101 | 10 | 104 | 1.63e-35 | 119 |
| MS.gene41686.t1 | AT2G27550 | 54.902 | 102 | 45 | 1 | 1 | 101 | 1 | 102 | 2.01e-34 | 116 |
| MS.gene41686.t1 | AT1G18100 | 52.632 | 95 | 44 | 1 | 3 | 97 | 2 | 95 | 2.42e-30 | 106 |
Find 36 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGCTGTAGGGCGTGTAAT+AGG | 0.236534 | 7.1:-20023317 | MS.gene41686:CDS |
| GAGCTCAAACCTTCTCAAAT+TGG | 0.266960 | 7.1:-20023218 | MS.gene41686:CDS |
| TCCTTAAAAGTGGGGTTACT+TGG | 0.279792 | 7.1:+20022990 | None:intergenic |
| TGGCTGTAGGGCGTGTAATA+GGG | 0.298942 | 7.1:-20023316 | MS.gene41686:CDS |
| TTACTTGGGCTAGGTGAATC+AGG | 0.383636 | 7.1:+20023005 | None:intergenic |
| AAAGTGACTTCAGTGGTTCC+TGG | 0.411425 | 7.1:+20022842 | None:intergenic |
| AGGTTGGTGACTGATATTCC+AGG | 0.439180 | 7.1:-20022860 | MS.gene41686:CDS |
| GGTAATAAAGATGTGAATAA+TGG | 0.441855 | 7.1:-20023245 | MS.gene41686:CDS |
| GTTTCCACCAACACTCACTC+TGG | 0.463855 | 7.1:+20023187 | None:intergenic |
| GGAACCACTGAAGTCACTTT+CGG | 0.499071 | 7.1:-20022839 | None:intergenic |
| CAACCCAGAGTGAGTGTTGG+TGG | 0.502018 | 7.1:-20023191 | MS.gene41686:CDS |
| CCTTAAAAGTGGGGTTACTT+GGG | 0.502208 | 7.1:+20022991 | None:intergenic |
| ATTCCTCTCCGAGTGACCTA+TGG | 0.511136 | 7.1:-20023266 | MS.gene41686:CDS |
| GTGGAGGTACTCCTTAAAAG+TGG | 0.534289 | 7.1:+20022980 | None:intergenic |
| GTAGGAATCCACTGGCTGTA+GGG | 0.534446 | 7.1:-20023328 | MS.gene41686:CDS |
| CTTTATTACCATAGGTCACT+CGG | 0.538470 | 7.1:+20023258 | None:intergenic |
| ATTCACATCTTTATTACCAT+AGG | 0.539018 | 7.1:+20023250 | None:intergenic |
| GAGAGGAATGGAACTTTCAA+AGG | 0.547771 | 7.1:+20023280 | None:intergenic |
| GGCTGTAGGGCGTGTAATAG+GGG | 0.552217 | 7.1:-20023315 | MS.gene41686:CDS |
| AAAGTGGGGTTACTTGGGCT+AGG | 0.558426 | 7.1:+20022996 | None:intergenic |
| TTTCCACCAACACTCACTCT+GGG | 0.558856 | 7.1:+20023188 | None:intergenic |
| CGGTAGCAGTAGGAATCCAC+TGG | 0.566544 | 7.1:-20023336 | MS.gene41686:CDS |
| GGTTGATTGCCAATTTGAGA+AGG | 0.568164 | 7.1:+20023209 | None:intergenic |
| TGGAGGTACTCCTTAAAAGT+GGG | 0.569324 | 7.1:+20022981 | None:intergenic |
| CACATATGGCCGGTAGCAGT+AGG | 0.586122 | 7.1:-20023346 | None:intergenic |
| TGTAAATTACTAGGGTGTAG+AGG | 0.586142 | 7.1:+20023153 | None:intergenic |
| ATAGGTCACTCGGAGAGGAA+TGG | 0.588640 | 7.1:+20023268 | None:intergenic |
| AACTGAGAACCACATATGGC+CGG | 0.594659 | 7.1:-20023356 | None:intergenic |
| AGTAGGAATCCACTGGCTGT+AGG | 0.611301 | 7.1:-20023329 | MS.gene41686:CDS |
| CAGTGGATTCCTACTGCTAC+CGG | 0.625296 | 7.1:+20023337 | None:intergenic |
| ATTACACGCCCTACAGCCAG+TGG | 0.629675 | 7.1:+20023320 | None:intergenic |
| TTACCATAGGTCACTCGGAG+AGG | 0.633443 | 7.1:+20023263 | None:intergenic |
| ATTGATTTACTCACCAGTGG+AGG | 0.648865 | 7.1:+20022964 | None:intergenic |
| GGAGGTACTCCTTAAAAGTG+GGG | 0.684138 | 7.1:+20022982 | None:intergenic |
| CATACCGAAAGTGACTTCAG+TGG | 0.687337 | 7.1:+20022835 | None:intergenic |
| AATCAACCCAGAGTGAGTGT+TGG | 0.706365 | 7.1:-20023194 | MS.gene41686:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTATATAATGTAAATTACT+AGG | + | chr7.1:20023040-20023059 | None:intergenic | 15.0% |
| !! | CTATATAATGTAAATTACTA+GGG | + | chr7.1:20023039-20023058 | None:intergenic | 15.0% |
| !! | TATTATTTAACAAAAGTCTT+AGG | + | chr7.1:20023270-20023289 | None:intergenic | 15.0% |
| !! | AGAAACTACATTATGATAAT+AGG | + | chr7.1:20023121-20023140 | None:intergenic | 20.0% |
| !!! | ATTTCTTGCTCTATTTTTAT+AGG | - | chr7.1:20023142-20023161 | MS.gene41686:intron | 20.0% |
| ! | ATTCACATCTTTATTACCAT+AGG | + | chr7.1:20022934-20022953 | None:intergenic | 25.0% |
| ! | GGTAATAAAGATGTGAATAA+TGG | - | chr7.1:20022936-20022955 | MS.gene41686:intron | 25.0% |
| !!! | TCTATTTTTATAGGTTATGG+TGG | - | chr7.1:20023151-20023170 | MS.gene41686:intron | 25.0% |
| !!! | TGCTCTATTTTTATAGGTTA+TGG | - | chr7.1:20023148-20023167 | MS.gene41686:intron | 25.0% |
| CTCTTCTCTCAATAAACTTT+AGG | - | chr7.1:20023301-20023320 | MS.gene41686:CDS | 30.0% | |
| TCTCTCAATAAACTTTAGGT+TGG | - | chr7.1:20023305-20023324 | MS.gene41686:CDS | 30.0% | |
| CTTTATTACCATAGGTCACT+CGG | + | chr7.1:20022926-20022945 | None:intergenic | 35.0% | |
| GGTAAATCATAAGCAGCATA+AGG | + | chr7.1:20023249-20023268 | None:intergenic | 35.0% | |
| TGTAAATTACTAGGGTGTAG+AGG | + | chr7.1:20023031-20023050 | None:intergenic | 35.0% | |
| ! | GTAATTGATTTACTCACCAG+TGG | + | chr7.1:20023223-20023242 | None:intergenic | 35.0% |
| CCTTAAAAGTGGGGTTACTT+GGG | + | chr7.1:20023193-20023212 | None:intergenic | 40.0% | |
| GAGAGGAATGGAACTTTCAA+AGG | + | chr7.1:20022904-20022923 | None:intergenic | 40.0% | |
| GAGCTCAAACCTTCTCAAAT+TGG | - | chr7.1:20022963-20022982 | MS.gene41686:intron | 40.0% | |
| GGTTGATTGCCAATTTGAGA+AGG | + | chr7.1:20022975-20022994 | None:intergenic | 40.0% | |
| TCCTTAAAAGTGGGGTTACT+TGG | + | chr7.1:20023194-20023213 | None:intergenic | 40.0% | |
| ! | ATTGATTTACTCACCAGTGG+AGG | + | chr7.1:20023220-20023239 | None:intergenic | 40.0% |
| !! | TGGAGGTACTCCTTAAAAGT+GGG | + | chr7.1:20023203-20023222 | None:intergenic | 40.0% |
| AAAGTGACTTCAGTGGTTCC+TGG | + | chr7.1:20023342-20023361 | None:intergenic | 45.0% | |
| AATCAACCCAGAGTGAGTGT+TGG | - | chr7.1:20022987-20023006 | MS.gene41686:CDS | 45.0% | |
| TTTCCACCAACACTCACTCT+GGG | + | chr7.1:20022996-20023015 | None:intergenic | 45.0% | |
| ! | TTACTTGGGCTAGGTGAATC+AGG | + | chr7.1:20023179-20023198 | None:intergenic | 45.0% |
| !! | AGGTTGGTGACTGATATTCC+AGG | - | chr7.1:20023321-20023340 | MS.gene41686:CDS | 45.0% |
| !! | CCCAAGTAACCCCACTTTTA+AGG | - | chr7.1:20023190-20023209 | MS.gene41686:CDS | 45.0% |
| !! | GGAGGTACTCCTTAAAAGTG+GGG | + | chr7.1:20023202-20023221 | None:intergenic | 45.0% |
| !! | GTGGAGGTACTCCTTAAAAG+TGG | + | chr7.1:20023204-20023223 | None:intergenic | 45.0% |
| !!! | CTTTTAAGGAGTACCTCCAC+TGG | - | chr7.1:20023204-20023223 | MS.gene41686:CDS | 45.0% |
| AAAGTGGGGTTACTTGGGCT+AGG | + | chr7.1:20023188-20023207 | None:intergenic | 50.0% | |
| ATAGGTCACTCGGAGAGGAA+TGG | + | chr7.1:20022916-20022935 | None:intergenic | 50.0% | |
| ATTCCTCTCCGAGTGACCTA+TGG | - | chr7.1:20022915-20022934 | MS.gene41686:intron | 50.0% | |
| GTTTCCACCAACACTCACTC+TGG | + | chr7.1:20022997-20023016 | None:intergenic | 50.0% | |
| TGGCTGTAGGGCGTGTAATA+GGG | - | chr7.1:20022865-20022884 | MS.gene41686:intron | 50.0% | |
| TTACCATAGGTCACTCGGAG+AGG | + | chr7.1:20022921-20022940 | None:intergenic | 50.0% | |
| ! | AGTAGGAATCCACTGGCTGT+AGG | - | chr7.1:20022852-20022871 | MS.gene41686:CDS | 50.0% |
| ! | CAGTGGATTCCTACTGCTAC+CGG | + | chr7.1:20022847-20022866 | None:intergenic | 50.0% |
| ! | GTAGGAATCCACTGGCTGTA+GGG | - | chr7.1:20022853-20022872 | MS.gene41686:CDS | 50.0% |
| ATTACACGCCCTACAGCCAG+TGG | + | chr7.1:20022864-20022883 | None:intergenic | 55.0% | |
| CGGTAGCAGTAGGAATCCAC+TGG | - | chr7.1:20022845-20022864 | MS.gene41686:CDS | 55.0% | |
| CTGGCTGTAGGGCGTGTAAT+AGG | - | chr7.1:20022864-20022883 | MS.gene41686:intron | 55.0% | |
| GGCTGTAGGGCGTGTAATAG+GGG | - | chr7.1:20022866-20022885 | MS.gene41686:intron | 55.0% | |
| !! | CAACCCAGAGTGAGTGTTGG+TGG | - | chr7.1:20022990-20023009 | MS.gene41686:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 20022840 | 20023363 | 20022840 | ID=MS.gene41686 |
| chr7.1 | mRNA | 20022840 | 20023363 | 20022840 | ID=MS.gene41686.t1;Parent=MS.gene41686 |
| chr7.1 | exon | 20023163 | 20023363 | 20023163 | ID=MS.gene41686.t1.exon1;Parent=MS.gene41686.t1 |
| chr7.1 | CDS | 20023163 | 20023363 | 20023163 | ID=cds.MS.gene41686.t1;Parent=MS.gene41686.t1 |
| chr7.1 | exon | 20022978 | 20023039 | 20022978 | ID=MS.gene41686.t1.exon2;Parent=MS.gene41686.t1 |
| chr7.1 | CDS | 20022978 | 20023039 | 20022978 | ID=cds.MS.gene41686.t1;Parent=MS.gene41686.t1 |
| chr7.1 | exon | 20022840 | 20022880 | 20022840 | ID=MS.gene41686.t1.exon3;Parent=MS.gene41686.t1 |
| chr7.1 | CDS | 20022840 | 20022880 | 20022840 | ID=cds.MS.gene41686.t1;Parent=MS.gene41686.t1 |
| Gene Sequence |
| Protein sequence |
