Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41806.t1 | XP_024625179.1 | 96.4 | 554 | 9 | 1 | 1 | 543 | 22 | 575 | 5.50E-299 | 1036.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41806.t1 | Q8CFK2 | 30.1 | 405 | 233 | 10 | 1 | 390 | 24 | 393 | 1.7e-38 | 162.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41806.t1 | G7L1K5 | 96.4 | 554 | 9 | 1 | 1 | 543 | 22 | 575 | 3.9e-299 | 1036.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057034 | MS.gene41806 | 0.802486 | 5.48E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 114 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATTTATGAAGCAAATT+TGG | 0.159053 | 7.4:-20914564 | MS.gene41806:CDS |
| AGCTGCTGCAGCTGCTTCCT+TGG | 0.214061 | 7.4:+20914592 | None:intergenic |
| TCATCGTTTGCTCACATATA+TGG | 0.252896 | 7.4:+20916882 | None:intergenic |
| AGCCTTACAGGAAGATAATA+TGG | 0.275857 | 7.4:-20914797 | MS.gene41806:CDS |
| TAATTATAACCATCTTCTTC+AGG | 0.283912 | 7.4:+20911890 | None:intergenic |
| CACATAGTCCAAGTGCAAAA+TGG | 0.287747 | 7.4:+20916597 | None:intergenic |
| TAGATCAAATCGTACTTTCT+TGG | 0.291217 | 7.4:+20912012 | None:intergenic |
| ATGAGGAAGAGAAGCCTTAC+AGG | 0.306360 | 7.4:-20914809 | MS.gene41806:CDS |
| GAAAATTCAATAAGAAAGTA+TGG | 0.316949 | 7.4:+20920650 | None:intergenic |
| AGTTAGATGCTTCACGTAAA+AGG | 0.317279 | 7.4:-20923068 | MS.gene41806:CDS |
| GACACGGTCTCGGGAGGATT+TGG | 0.325691 | 7.4:-20916461 | MS.gene41806:CDS |
| GGGAGGATTTGGTGGTGGTT+TGG | 0.338991 | 7.4:-20916450 | MS.gene41806:CDS |
| TGTTTAATAGAGCAAAATAT+CGG | 0.340160 | 7.4:-20923115 | MS.gene41806:intron |
| TGCATTCCAGCGAGCTGAAC+AGG | 0.340722 | 7.4:-20916420 | MS.gene41806:CDS |
| GCTTACAGCTTCTAAAGAAA+TGG | 0.345137 | 7.4:-20922619 | MS.gene41806:intron |
| GATTTGCTTGGCCATAGAAA+TGG | 0.350863 | 7.4:-20919935 | MS.gene41806:CDS |
| CGTGTCTTTATCTTGCATTC+CGG | 0.358135 | 7.4:-20922369 | MS.gene41806:intron |
| GCTCAGTAACAAAGTCAATA+TGG | 0.359169 | 7.4:-20912580 | MS.gene41806:CDS |
| AGTCAAGAAAACAAATTCTA+AGG | 0.361498 | 7.4:-20916393 | MS.gene41806:CDS |
| CAGAGACAACCCCATTTCTA+TGG | 0.363090 | 7.4:+20919924 | None:intergenic |
| CTCTCTCCTGTTCAGCTCGC+TGG | 0.366469 | 7.4:+20916414 | None:intergenic |
| TGATAATCACGATGATGAAC+TGG | 0.367329 | 7.4:-20911984 | MS.gene41806:CDS |
| AGATCAAATCGTACTTTCTT+GGG | 0.368877 | 7.4:+20912013 | None:intergenic |
| TGCAGCTTTGTGAAGTGTTA+AGG | 0.378656 | 7.4:-20920489 | MS.gene41806:CDS |
| ACATAGTCCAAGTGCAAAAT+GGG | 0.386704 | 7.4:+20916598 | None:intergenic |
| TTGGTATAATAATGAAAATA+TGG | 0.391550 | 7.4:-20911927 | MS.gene41806:CDS |
| GCCTCTGCAGCAGATTGAGC+AGG | 0.395396 | 7.4:+20912829 | None:intergenic |
| ATTTGCTTGGCCATAGAAAT+GGG | 0.398488 | 7.4:-20919934 | MS.gene41806:CDS |
| TTACTGTTTGGCATTACAAC+CGG | 0.417781 | 7.4:+20916677 | None:intergenic |
| TCCCATATTATCTTCCTGTA+AGG | 0.418087 | 7.4:+20914795 | None:intergenic |
| GATCCGTTGAATTTACTGTT+TGG | 0.423755 | 7.4:+20916665 | None:intergenic |
| TCAGGGTTTGTACATCTTGT+GGG | 0.430358 | 7.4:-20924291 | None:intergenic |
| GAGATGTGGTCCGCCAAGCT+AGG | 0.437701 | 7.4:-20922563 | MS.gene41806:CDS |
| AGATAAAGAGAAGGATCAAT+AGG | 0.439509 | 7.4:+20920439 | None:intergenic |
| AATTATAACCATCTTCTTCA+GGG | 0.445106 | 7.4:+20911891 | None:intergenic |
| GTCAGGGTTTGTACATCTTG+TGG | 0.447838 | 7.4:-20924292 | None:intergenic |
| GCTTCTCTTCCTCATTGAAA+AGG | 0.451934 | 7.4:+20914817 | None:intergenic |
| TTGAGGATGGTGACAATGAT+TGG | 0.452380 | 7.4:-20911946 | MS.gene41806:CDS |
| TGCAGAGGCTGCTTGCCAAA+TGG | 0.454248 | 7.4:-20912815 | MS.gene41806:CDS |
| GACGTAAATCAGAGCAAGTA+CGG | 0.455951 | 7.4:-20922396 | MS.gene41806:CDS |
| AGTGTGTGAGCACAAAGAAA+TGG | 0.459697 | 7.4:-20916627 | MS.gene41806:CDS |
| ACGGAAGCTCAAGATGAATC+AGG | 0.460758 | 7.4:-20915899 | MS.gene41806:CDS |
| GCCTGCTCAATCTGCTGCAG+AGG | 0.461546 | 7.4:-20912830 | MS.gene41806:CDS |
| ATATTAACATACTTCATAGA+AGG | 0.466151 | 7.4:+20922539 | None:intergenic |
| GGTTTACATGTAGATTTGCT+TGG | 0.467201 | 7.4:-20919947 | MS.gene41806:intron |
| AATGAACCGTGACTGGTTGC+AGG | 0.469326 | 7.4:-20919876 | MS.gene41806:intron |
| TGTTCAGCTCGCTGGAATGC+AGG | 0.470422 | 7.4:+20916422 | None:intergenic |
| CGTAAAAGGACTTTATATAG+AGG | 0.475221 | 7.4:-20923054 | MS.gene41806:intron |
| TTGCTCTGATTTACGTCCTC+GGG | 0.475642 | 7.4:+20922402 | None:intergenic |
| TTACGCAAATGAACCGTGAC+TGG | 0.476466 | 7.4:-20919883 | MS.gene41806:CDS |
| TTGTTGTGCAGTTATGAGCT+TGG | 0.479081 | 7.4:-20920523 | MS.gene41806:intron |
| GTAACTTACAATATCTCGCT+TGG | 0.480813 | 7.4:+20919635 | None:intergenic |
| TTACAGTTTGACACGGTCTC+GGG | 0.482762 | 7.4:-20916470 | MS.gene41806:intron |
| TCATATTTCCCTGAAGAAGA+TGG | 0.486824 | 7.4:-20911899 | MS.gene41806:CDS |
| GTCTCGGGAGGATTTGGTGG+TGG | 0.492886 | 7.4:-20916455 | MS.gene41806:CDS |
| TACAGCTCTCTTGATTCCAC+TGG | 0.493925 | 7.4:+20914533 | None:intergenic |
| CTTGCTCTGATTTACGTCCT+CGG | 0.506138 | 7.4:+20922401 | None:intergenic |
| GATGGTTATAATTATGATGA+GGG | 0.508705 | 7.4:-20911881 | MS.gene41806:CDS |
| TTTACAGTTTGACACGGTCT+CGG | 0.512800 | 7.4:-20916471 | MS.gene41806:intron |
| ACGGTCTCGGGAGGATTTGG+TGG | 0.517103 | 7.4:-20916458 | MS.gene41806:CDS |
| CTTTGTGCTCACACACTAGA+GGG | 0.519934 | 7.4:+20916633 | None:intergenic |
| GGCATCAAATAGCGCATGCA+AGG | 0.523242 | 7.4:-20915939 | MS.gene41806:CDS |
| CATAGAAGGCCCTAGCTTGG+CGG | 0.525773 | 7.4:+20922553 | None:intergenic |
| AGATGTGGTCCGCCAAGCTA+GGG | 0.529307 | 7.4:-20922562 | MS.gene41806:CDS |
| CTTTCAGGAGCAAGCAGCCA+AGG | 0.531731 | 7.4:-20914609 | MS.gene41806:intron |
| AAATAGCGCATGCAAGGGAG+GGG | 0.532095 | 7.4:-20915933 | MS.gene41806:CDS |
| TTCTGTGGCAAAATCAAGAA+AGG | 0.534234 | 7.4:-20914495 | MS.gene41806:intron |
| GAATATTTAAGTCTCAGCCT+TGG | 0.535149 | 7.4:-20922597 | MS.gene41806:CDS |
| GCCTTACAGGAAGATAATAT+GGG | 0.537814 | 7.4:-20914796 | MS.gene41806:CDS |
| TTGCCAAATGGTGAAAAGAA+AGG | 0.548531 | 7.4:-20912803 | MS.gene41806:intron |
| AAACAGACAGGGAGGAAACC+TGG | 0.548805 | 7.4:-20919714 | MS.gene41806:intron |
| TTGTGCAGTTATGAGCTTGG+AGG | 0.548966 | 7.4:-20920520 | MS.gene41806:intron |
| CTTCATAGAAGGCCCTAGCT+TGG | 0.550801 | 7.4:+20922550 | None:intergenic |
| GTTTAATAGAGCAAAATATC+GGG | 0.553849 | 7.4:-20923114 | MS.gene41806:intron |
| TGAATCTACCTCAGCTTCTG+TGG | 0.555464 | 7.4:-20914510 | MS.gene41806:CDS |
| CAAATAGCGCATGCAAGGGA+GGG | 0.557985 | 7.4:-20915934 | MS.gene41806:CDS |
| CGATGATGAACTGGGATCAG+CGG | 0.561005 | 7.4:-20911975 | MS.gene41806:CDS |
| ACGTAAATCAGAGCAAGTAC+GGG | 0.566647 | 7.4:-20922395 | MS.gene41806:CDS |
| TCAAATAGCGCATGCAAGGG+AGG | 0.567776 | 7.4:-20915935 | MS.gene41806:CDS |
| TTTAGTAAAAGTCGGCTCCT+CGG | 0.570896 | 7.4:+20924243 | None:intergenic |
| CTCAGCCTTGGAGTAAGTGA+GGG | 0.572595 | 7.4:-20922585 | MS.gene41806:CDS |
| GGTTTGTACATCTTGTGGGA+AGG | 0.574175 | 7.4:-20924287 | MS.gene41806:CDS |
| TACATCTTGTGGGAAGGTGT+TGG | 0.574735 | 7.4:-20924281 | MS.gene41806:CDS |
| TCTCAGCCTTGGAGTAAGTG+AGG | 0.575387 | 7.4:-20922586 | MS.gene41806:CDS |
| AGATGGTTATAATTATGATG+AGG | 0.579920 | 7.4:-20911882 | MS.gene41806:CDS |
| TCTTTGTGCTCACACACTAG+AGG | 0.582080 | 7.4:+20916632 | None:intergenic |
| CAGTCACCTGCAACCAGTCA+CGG | 0.587214 | 7.4:+20919870 | None:intergenic |
| AAATTTGATAAAAGCAGTTG+AGG | 0.589359 | 7.4:-20923091 | MS.gene41806:CDS |
| TCAGCTCGCTGGAATGCAGG+AGG | 0.591458 | 7.4:+20916425 | None:intergenic |
| CTCTGATATTGATGACCAAG+AGG | 0.592332 | 7.4:-20915870 | MS.gene41806:intron |
| GGGAGGGGATGACACTAGTA+CGG | 0.593649 | 7.4:-20915918 | MS.gene41806:CDS |
| TGGAGTAAGTGAGGGAGATG+TGG | 0.601914 | 7.4:-20922577 | MS.gene41806:CDS |
| GATAATCACGATGATGAACT+GGG | 0.603034 | 7.4:-20911983 | MS.gene41806:CDS |
| TTTGTGCTCACACACTAGAG+GGG | 0.608810 | 7.4:+20916634 | None:intergenic |
| CAGTTTGACACGGTCTCGGG+AGG | 0.610904 | 7.4:-20916467 | MS.gene41806:CDS |
| TATTTATGAAGATAAAGAGA+AGG | 0.612010 | 7.4:+20920430 | None:intergenic |
| TCAGAACCGCGAGTATCTTG+AGG | 0.625907 | 7.4:-20914769 | MS.gene41806:intron |
| CATCTCCCTCACTTACTCCA+AGG | 0.626580 | 7.4:+20922580 | None:intergenic |
| ACTATGTGAAACTTGTTACA+AGG | 0.628752 | 7.4:-20916582 | MS.gene41806:CDS |
| CAGCTCGCTGGAATGCAGGA+GGG | 0.634000 | 7.4:+20916426 | None:intergenic |
| ATGACCATGTGCATTAGCAG+AGG | 0.635109 | 7.4:+20919665 | None:intergenic |
| ATGCCAAACAGTAAATTCAA+CGG | 0.635396 | 7.4:-20916668 | MS.gene41806:CDS |
| GCAGCAGCAAATAATCCACC+AGG | 0.647321 | 7.4:+20919696 | None:intergenic |
| GCATCAAATAGCGCATGCAA+GGG | 0.649630 | 7.4:-20915938 | MS.gene41806:CDS |
| TTTGCTTGGCCATAGAAATG+GGG | 0.652044 | 7.4:-20919933 | MS.gene41806:CDS |
| TACACCTCTGCTAATGCACA+TGG | 0.652050 | 7.4:-20919669 | MS.gene41806:CDS |
| AACTTCAATTACCTGTCCGG+CGG | 0.654079 | 7.4:+20924216 | None:intergenic |
| GGTTGAGGAGTTGAATGCAA+TGG | 0.676513 | 7.4:-20916720 | MS.gene41806:CDS |
| CATAGTCCAAGTGCAAAATG+GGG | 0.677751 | 7.4:+20916599 | None:intergenic |
| TCTGTACCTCAAGATACTCG+CGG | 0.681039 | 7.4:+20914763 | None:intergenic |
| GATACATTTACAGTTTGACA+CGG | 0.682926 | 7.4:-20916477 | MS.gene41806:intron |
| AAATTTGGAAAATTGTCCAG+TGG | 0.694574 | 7.4:-20914549 | MS.gene41806:CDS |
| ACGTTTGAAAACAGACAGGG+AGG | 0.741511 | 7.4:-20919722 | MS.gene41806:intron |
| CAGACAGGGAGGAAACCTGG+TGG | 0.780406 | 7.4:-20919711 | MS.gene41806:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 20911871 | 20924309 | 20911871 | ID=MS.gene41806 |
| chr7.4 | mRNA | 20911871 | 20924309 | 20911871 | ID=MS.gene41806.t1;Parent=MS.gene41806 |
| chr7.4 | exon | 20924228 | 20924309 | 20924228 | ID=MS.gene41806.t1.exon1;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20924228 | 20924309 | 20924228 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20923055 | 20923127 | 20923055 | ID=MS.gene41806.t1.exon2;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20923055 | 20923127 | 20923055 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20922551 | 20922633 | 20922551 | ID=MS.gene41806.t1.exon3;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20922551 | 20922633 | 20922551 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20922370 | 20922446 | 20922370 | ID=MS.gene41806.t1.exon4;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20922370 | 20922446 | 20922370 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20920629 | 20920685 | 20920629 | ID=MS.gene41806.t1.exon5;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20920629 | 20920685 | 20920629 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20920422 | 20920534 | 20920422 | ID=MS.gene41806.t1.exon6;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20920422 | 20920534 | 20920422 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20919877 | 20919956 | 20919877 | ID=MS.gene41806.t1.exon7;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20919877 | 20919956 | 20919877 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20919644 | 20919730 | 20919644 | ID=MS.gene41806.t1.exon8;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20919644 | 20919730 | 20919644 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20916835 | 20916915 | 20916835 | ID=MS.gene41806.t1.exon9;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20916835 | 20916915 | 20916835 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20916580 | 20916741 | 20916580 | ID=MS.gene41806.t1.exon10;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20916580 | 20916741 | 20916580 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20916382 | 20916486 | 20916382 | ID=MS.gene41806.t1.exon11;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20916382 | 20916486 | 20916382 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20915871 | 20915969 | 20915871 | ID=MS.gene41806.t1.exon12;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20915871 | 20915969 | 20915871 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20914770 | 20914850 | 20914770 | ID=MS.gene41806.t1.exon13;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20914770 | 20914850 | 20914770 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20914496 | 20914624 | 20914496 | ID=MS.gene41806.t1.exon14;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20914496 | 20914624 | 20914496 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20912804 | 20912890 | 20912804 | ID=MS.gene41806.t1.exon15;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20912804 | 20912890 | 20912804 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20912548 | 20912604 | 20912548 | ID=MS.gene41806.t1.exon16;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20912548 | 20912604 | 20912548 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| chr7.4 | exon | 20911871 | 20912050 | 20911871 | ID=MS.gene41806.t1.exon17;Parent=MS.gene41806.t1 |
| chr7.4 | CDS | 20911871 | 20912050 | 20911871 | ID=cds.MS.gene41806.t1;Parent=MS.gene41806.t1 |
| Gene Sequence |
| Protein sequence |
