Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41864.t1 | KEH35414.1 | 92.4 | 131 | 9 | 1 | 1 | 131 | 203 | 332 | 1.30E-60 | 242.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41864.t1 | Q41009 | 83.6 | 110 | 18 | 0 | 1 | 110 | 201 | 310 | 1.1e-49 | 197.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41864.t1 | A0A072V091 | 92.4 | 131 | 9 | 1 | 1 | 131 | 203 | 332 | 9.2e-61 | 242.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene41864 | 0.80472 | 1.89E-49 | -1.69E-46 |
| MS.gene050916 | MS.gene41864 | 0.803656 | 3.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41864.t1 | MTR_3g090160 | 92.366 | 131 | 9 | 1 | 1 | 131 | 203 | 332 | 2.62e-83 | 247 |
| MS.gene41864.t1 | MTR_7g088050 | 88.550 | 131 | 15 | 0 | 1 | 131 | 202 | 332 | 2.58e-82 | 244 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41864.t1 | AT5G05000 | 58.491 | 106 | 44 | 0 | 3 | 108 | 203 | 308 | 3.04e-43 | 144 |
| MS.gene41864.t1 | AT5G05000 | 58.491 | 106 | 44 | 0 | 3 | 108 | 203 | 308 | 3.04e-43 | 144 |
| MS.gene41864.t1 | AT5G05000 | 58.491 | 106 | 44 | 0 | 3 | 108 | 203 | 308 | 3.04e-43 | 144 |
| MS.gene41864.t1 | AT1G02280 | 51.020 | 98 | 47 | 1 | 2 | 99 | 200 | 296 | 4.28e-28 | 104 |
| MS.gene41864.t1 | AT1G02280 | 51.020 | 98 | 47 | 1 | 2 | 99 | 200 | 296 | 4.28e-28 | 104 |
Find 25 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCAATTTCTGTGATTGTT+TGG | 0.334323 | 7.4:+20242207 | None:intergenic |
| TTCTTCCAAATGGGATTGCT+TGG | 0.375270 | 7.4:-20242244 | MS.gene41864:CDS |
| TTTCCTCTCTGATTTGGATT+CGG | 0.383451 | 7.4:+20242137 | None:intergenic |
| TCCTGTCATATTTGCTCTAC+AGG | 0.390727 | 7.4:-20242105 | MS.gene41864:intron |
| GGAATCCAAGCAATCCCATT+TGG | 0.403774 | 7.4:+20242239 | None:intergenic |
| TGGTGACAGGTTCTTCCAAA+TGG | 0.415072 | 7.4:-20242254 | MS.gene41864:intron |
| GTTGACAAGAATTTGATTGA+AGG | 0.442484 | 7.4:-20242161 | MS.gene41864:CDS |
| CGTAAAGAGAAGCGTGGTGC+AGG | 0.446680 | 7.4:-20241862 | MS.gene41864:CDS |
| TTCTTGATCAGTGCTTCTAT+AGG | 0.477157 | 7.4:+20241973 | None:intergenic |
| CTGTTCTCAATCAAGACAAC+AGG | 0.482067 | 7.4:+20242403 | None:intergenic |
| GGTGACAGGTTCTTCCAAAT+GGG | 0.497063 | 7.4:-20242253 | MS.gene41864:intron |
| ACCTGTAGAGCAAATATGAC+AGG | 0.529529 | 7.4:+20242104 | None:intergenic |
| TTTCTGTGATTGTTTGGACT+AGG | 0.564078 | 7.4:+20242213 | None:intergenic |
| CTCAATCAAGACAACAGGAA+TGG | 0.587478 | 7.4:+20242408 | None:intergenic |
| CAGAAAGAGACGCGTGAAGG+TGG | 0.593888 | 7.4:-20241898 | MS.gene41864:CDS |
| TTGTCTTGATTGAGAACAGT+GGG | 0.596287 | 7.4:-20242399 | MS.gene41864:CDS |
| TCTTGGGAGACACGTGATGT+CGG | 0.608203 | 7.4:-20241925 | MS.gene41864:CDS |
| ACATCACGTGTCTCCCAAGA+GGG | 0.623468 | 7.4:+20241928 | None:intergenic |
| GTGATTGTTTGGACTAGGTG+AGG | 0.644553 | 7.4:+20242218 | None:intergenic |
| GACATCACGTGTCTCCCAAG+AGG | 0.672049 | 7.4:+20241927 | None:intergenic |
| GTTCAGAAAGAGACGCGTGA+AGG | 0.686151 | 7.4:-20241901 | MS.gene41864:CDS |
| GTTGTCTTGATTGAGAACAG+TGG | 0.701119 | 7.4:-20242400 | MS.gene41864:CDS |
| TCTTGATTGAGAACAGTGGG+AGG | 0.720229 | 7.4:-20242396 | MS.gene41864:CDS |
| GGACCGAATCCAAATCAGAG+AGG | 0.731341 | 7.4:-20242140 | MS.gene41864:CDS |
| GATACGCGTAAAGAGAAGCG+TGG | 0.744582 | 7.4:-20241868 | MS.gene41864:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGAAGAATAAAATAAACAAA+AGG | + | chr7.4:20242244-20242263 | None:intergenic | 20.0% |
| ! | ATCAGATTAAGCAAGATAAA+TGG | + | chr7.4:20241974-20241993 | None:intergenic | 25.0% |
| ! | CAAAAATGAAACTGATGAAA+AGG | - | chr7.4:20241896-20241915 | MS.gene41864:CDS | 25.0% |
| ! | TCAGATTAAGCAAGATAAAT+GGG | + | chr7.4:20241973-20241992 | None:intergenic | 25.0% |
| AATGAAACTGATGAAAAGGT+TGG | - | chr7.4:20241900-20241919 | MS.gene41864:CDS | 30.0% | |
| ATGCAATTTCTGTGATTGTT+TGG | + | chr7.4:20242060-20242079 | None:intergenic | 30.0% | |
| CAAATCAGAGAGGAAAAATA+TGG | - | chr7.4:20242134-20242153 | MS.gene41864:CDS | 30.0% | |
| GTTGACAAGAATTTGATTGA+AGG | - | chr7.4:20242103-20242122 | MS.gene41864:intron | 30.0% | |
| ! | CATATTTTTCCTCTCTGATT+TGG | + | chr7.4:20242136-20242155 | None:intergenic | 30.0% |
| TAATGAGAAAAAGCCCTCTT+GGG | - | chr7.4:20242323-20242342 | MS.gene41864:intron | 35.0% | |
| TGATTGAGAAAATGTGTGCA+TGG | - | chr7.4:20241990-20242009 | MS.gene41864:CDS | 35.0% | |
| TTCTTGATCAGTGCTTCTAT+AGG | + | chr7.4:20242294-20242313 | None:intergenic | 35.0% | |
| TTGTCTTGATTGAGAACAGT+GGG | - | chr7.4:20241865-20241884 | MS.gene41864:CDS | 35.0% | |
| TTTCTGTGATTGTTTGGACT+AGG | + | chr7.4:20242054-20242073 | None:intergenic | 35.0% | |
| ! | AGGTAAGCTTTTGAACTTTG+AGG | - | chr7.4:20242179-20242198 | MS.gene41864:CDS | 35.0% |
| !! | GATAAATGGGTTGATAGAAG+AGG | + | chr7.4:20241960-20241979 | None:intergenic | 35.0% |
| !! | GGTTGATAGAAGAGGATATA+AGG | + | chr7.4:20241952-20241971 | None:intergenic | 35.0% |
| !! | TTTCCTCTCTGATTTGGATT+CGG | + | chr7.4:20242130-20242149 | None:intergenic | 35.0% |
| ACCTGTAGAGCAAATATGAC+AGG | + | chr7.4:20242163-20242182 | None:intergenic | 40.0% | |
| CTAATGAGAAAAAGCCCTCT+TGG | - | chr7.4:20242322-20242341 | MS.gene41864:intron | 40.0% | |
| CTCAATCAAGACAACAGGAA+TGG | + | chr7.4:20241859-20241878 | None:intergenic | 40.0% | |
| CTGTTCTCAATCAAGACAAC+AGG | + | chr7.4:20241864-20241883 | None:intergenic | 40.0% | |
| GTTGTCTTGATTGAGAACAG+TGG | - | chr7.4:20241864-20241883 | MS.gene41864:CDS | 40.0% | |
| TCCTGTCATATTTGCTCTAC+AGG | - | chr7.4:20242159-20242178 | MS.gene41864:CDS | 40.0% | |
| TTCTTCCAAATGGGATTGCT+TGG | - | chr7.4:20242020-20242039 | MS.gene41864:intron | 40.0% | |
| ! | AAGGTTGGAGATTTGCTTCA+TGG | - | chr7.4:20241915-20241934 | MS.gene41864:CDS | 40.0% |
| GAAAACCAAGAACTGCAGAG+AGG | + | chr7.4:20242265-20242284 | None:intergenic | 45.0% | |
| GAAAATGTGTGCATGGTGAC+AGG | - | chr7.4:20241997-20242016 | MS.gene41864:intron | 45.0% | |
| GGAATCCAAGCAATCCCATT+TGG | + | chr7.4:20242028-20242047 | None:intergenic | 45.0% | |
| GGTGACAGGTTCTTCCAAAT+GGG | - | chr7.4:20242011-20242030 | MS.gene41864:intron | 45.0% | |
| TCTTGATTGAGAACAGTGGG+AGG | - | chr7.4:20241868-20241887 | MS.gene41864:CDS | 45.0% | |
| TGGTGACAGGTTCTTCCAAA+TGG | - | chr7.4:20242010-20242029 | MS.gene41864:intron | 45.0% | |
| TTCTTCCTCTCTGCAGTTCT+TGG | - | chr7.4:20242257-20242276 | MS.gene41864:intron | 45.0% | |
| ! | GTGATTGTTTGGACTAGGTG+AGG | + | chr7.4:20242049-20242068 | None:intergenic | 45.0% |
| ACATCACGTGTCTCCCAAGA+GGG | + | chr7.4:20242339-20242358 | None:intergenic | 50.0% | |
| GATACGCGTAAAGAGAAGCG+TGG | - | chr7.4:20242396-20242415 | MS.gene41864:CDS | 50.0% | |
| GGACCGAATCCAAATCAGAG+AGG | - | chr7.4:20242124-20242143 | MS.gene41864:CDS | 50.0% | |
| GTTCAGAAAGAGACGCGTGA+AGG | - | chr7.4:20242363-20242382 | MS.gene41864:intron | 50.0% | |
| TCTTGGGAGACACGTGATGT+CGG | - | chr7.4:20242339-20242358 | MS.gene41864:intron | 50.0% | |
| !! | AGAAGCGTGGTGCAGGTTTT+CGG | - | chr7.4:20242409-20242428 | MS.gene41864:CDS | 50.0% |
| CAGAAAGAGACGCGTGAAGG+TGG | - | chr7.4:20242366-20242385 | MS.gene41864:intron | 55.0% | |
| GACATCACGTGTCTCCCAAG+AGG | + | chr7.4:20242340-20242359 | None:intergenic | 55.0% | |
| ! | CGTAAAGAGAAGCGTGGTGC+AGG | - | chr7.4:20242402-20242421 | MS.gene41864:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 20241849 | 20242437 | 20241849 | ID=MS.gene41864 |
| chr7.4 | mRNA | 20241849 | 20242437 | 20241849 | ID=MS.gene41864.t1;Parent=MS.gene41864 |
| chr7.4 | exon | 20242369 | 20242437 | 20242369 | ID=MS.gene41864.t1.exon1;Parent=MS.gene41864.t1 |
| chr7.4 | CDS | 20242369 | 20242437 | 20242369 | ID=cds.MS.gene41864.t1;Parent=MS.gene41864.t1 |
| chr7.4 | exon | 20242106 | 20242267 | 20242106 | ID=MS.gene41864.t1.exon2;Parent=MS.gene41864.t1 |
| chr7.4 | CDS | 20242106 | 20242267 | 20242106 | ID=cds.MS.gene41864.t1;Parent=MS.gene41864.t1 |
| chr7.4 | exon | 20241849 | 20242013 | 20241849 | ID=MS.gene41864.t1.exon3;Parent=MS.gene41864.t1 |
| chr7.4 | CDS | 20241849 | 20242013 | 20241849 | ID=cds.MS.gene41864.t1;Parent=MS.gene41864.t1 |
| Gene Sequence |
| Protein sequence |
