Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42085.t1 | XP_024626508.1 | 97.3 | 74 | 2 | 0 | 1 | 74 | 54 | 127 | 1.10E-29 | 138.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42085.t1 | G7KX21 | 97.3 | 74 | 2 | 0 | 1 | 74 | 59 | 132 | 8.1e-30 | 138.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052164 | MS.gene42085 | 0.801991 | 6.93E-49 | -1.69E-46 |
| MS.gene053912 | MS.gene42085 | 0.801674 | 8.05E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene42085 | 0.811815 | 5.80E-51 | -1.69E-46 |
| MS.gene05494 | MS.gene42085 | 0.803598 | 3.23E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42085.t1 | MTR_7g072470 | 97.297 | 74 | 2 | 0 | 1 | 74 | 59 | 132 | 8.33e-47 | 145 |
| MS.gene42085.t1 | MTR_5g083810 | 58.108 | 74 | 30 | 1 | 1 | 73 | 104 | 177 | 1.23e-19 | 77.8 |
| MS.gene42085.t1 | MTR_3g466150 | 56.944 | 72 | 28 | 1 | 1 | 69 | 80 | 151 | 4.81e-18 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42085.t1 | AT3G56220 | 59.420 | 69 | 27 | 1 | 1 | 68 | 81 | 149 | 7.87e-22 | 82.8 |
| MS.gene42085.t1 | AT2G40435 | 57.353 | 68 | 29 | 0 | 1 | 68 | 81 | 148 | 9.20e-22 | 82.8 |
| MS.gene42085.t1 | AT2G40435 | 68.889 | 45 | 14 | 0 | 1 | 45 | 81 | 125 | 2.93e-17 | 70.5 |
| MS.gene42085.t1 | AT2G40435 | 68.889 | 45 | 14 | 0 | 1 | 45 | 81 | 125 | 3.18e-17 | 70.5 |
| MS.gene42085.t1 | AT3G56220 | 65.217 | 46 | 16 | 0 | 1 | 46 | 81 | 126 | 3.00e-16 | 68.2 |
Find 13 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATGATTAATGTGCTTT+TGG | 0.236031 | 7.2:-31940226 | None:intergenic |
| TGGTATGCTGGTATCAATAC+TGG | 0.411863 | 7.2:-31940190 | MS.gene42085:CDS |
| TTATTGTATGCAGAGTCATT+TGG | 0.420367 | 7.2:-31938826 | MS.gene42085:intron |
| CTCCTCCAACAGCTTCTAGC+TGG | 0.462141 | 7.2:+31940106 | None:intergenic |
| AGTATTGATACCAGCATACC+AGG | 0.467026 | 7.2:+31940192 | None:intergenic |
| AAAGAATAGCCCTGGTATGC+TGG | 0.482593 | 7.2:-31940202 | MS.gene42085:CDS |
| GACTTGATGTACTTGATGCT+AGG | 0.505174 | 7.2:-31940149 | MS.gene42085:CDS |
| ACTTGATGTACTTGATGCTA+GGG | 0.510074 | 7.2:-31940148 | MS.gene42085:CDS |
| ACTTTCCAGCTAGAAGCTGT+TGG | 0.533098 | 7.2:-31940111 | MS.gene42085:CDS |
| TTCCAGCTAGAAGCTGTTGG+AGG | 0.538408 | 7.2:-31940108 | MS.gene42085:CDS |
| CGATAGTATCAATGCGCAAG+TGG | 0.613229 | 7.2:-31938802 | MS.gene42085:CDS |
| GTATTGATACCAGCATACCA+GGG | 0.698292 | 7.2:+31940193 | None:intergenic |
| GTTGCAAGCAATCAAGAACA+CGG | 0.713497 | 7.2:-31938766 | MS.gene42085:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTTAAATCAAATTATAAA+TGG | + | chr7.2:31939823-31939842 | None:intergenic | 10.0% |
| !!! | TTTAATACTTTGTTTTTTAT+TGG | - | chr7.2:31939540-31939559 | MS.gene42085:intron | 10.0% |
| !! | AAATATTTGGTTATACAATT+TGG | - | chr7.2:31939198-31939217 | MS.gene42085:intron | 15.0% |
| !! | AATATTTGGTTATACAATTT+GGG | - | chr7.2:31939199-31939218 | MS.gene42085:intron | 15.0% |
| !!! | ATAAAATATATATGATGATG+AGG | - | chr7.2:31940091-31940110 | MS.gene42085:intron | 15.0% |
| !!! | GCTTTATAAATAATTTTAAG+TGG | - | chr7.2:31938954-31938973 | MS.gene42085:intron | 15.0% |
| !!! | TAAGTATTTTTCATGAATAT+TGG | - | chr7.2:31939279-31939298 | MS.gene42085:intron | 15.0% |
| !!! | TCTAATTTTGATTTTTTCTA+AGG | - | chr7.2:31939922-31939941 | MS.gene42085:intron | 15.0% |
| !!! | TGAATATTTTTCATGAATAT+TGG | - | chr7.2:31939339-31939358 | MS.gene42085:intron | 15.0% |
| !! | AAGTATATATACTTATCAAG+AGG | - | chr7.2:31939486-31939505 | MS.gene42085:intron | 20.0% |
| !! | ATAAAGCAATAAAGAGATTA+AGG | + | chr7.2:31938941-31938960 | None:intergenic | 20.0% |
| !! | CTACTAACAAACAAAAATTT+GGG | - | chr7.2:31939775-31939794 | MS.gene42085:intron | 20.0% |
| !! | TCTACTAACAAACAAAAATT+TGG | - | chr7.2:31939774-31939793 | MS.gene42085:intron | 20.0% |
| !! | TTAAAACCAATATAAAGAAG+AGG | - | chr7.2:31939011-31939030 | MS.gene42085:intron | 20.0% |
| !! | TTAATTCAATTTGAGTAAGA+TGG | - | chr7.2:31939750-31939769 | MS.gene42085:intron | 20.0% |
| !!! | ATACCTATTTTTCTTTCATT+CGG | - | chr7.2:31939961-31939980 | MS.gene42085:intron | 20.0% |
| ! | AATATTGAAGAAGTCTTTCA+CGG | - | chr7.2:31939171-31939190 | MS.gene42085:intron | 25.0% |
| ! | ATTATTATGTTGTTGCCTAA+AGG | - | chr7.2:31940052-31940071 | MS.gene42085:intron | 25.0% |
| ! | ATTCCGAATGAAAGAAAAAT+AGG | + | chr7.2:31939967-31939986 | None:intergenic | 25.0% |
| ! | GTGAGTGTATATATATGTTA+AGG | - | chr7.2:31939059-31939078 | MS.gene42085:intron | 25.0% |
| ! | TAAGAACCTCTTCTTTATAT+TGG | + | chr7.2:31939020-31939039 | None:intergenic | 25.0% |
| ! | TACCAATATTTAACACCTTT+AGG | + | chr7.2:31940070-31940089 | None:intergenic | 25.0% |
| ! | TTATTATCAGTCATTTCAAG+TGG | + | chr7.2:31939402-31939421 | None:intergenic | 25.0% |
| ! | TTCAATATTTATATACCCGA+TGG | + | chr7.2:31939160-31939179 | None:intergenic | 25.0% |
| !! | AAAGGAGTATATAATTCCAT+CGG | - | chr7.2:31939141-31939160 | MS.gene42085:intron | 25.0% |
| TCAACAAGAATCATATGAGA+GGG | + | chr7.2:31940125-31940144 | None:intergenic | 30.0% | |
| TCATGAAAAATCCTGCATAT+GGG | + | chr7.2:31939309-31939328 | None:intergenic | 30.0% | |
| TTATTGTATGCAGAGTCATT+TGG | - | chr7.2:31940148-31940167 | MS.gene42085:CDS | 30.0% | |
| TTCAACAAGAATCATATGAG+AGG | + | chr7.2:31940126-31940145 | None:intergenic | 30.0% | |
| ! | AAGGAGTATATAATTCCATC+GGG | - | chr7.2:31939142-31939161 | MS.gene42085:intron | 30.0% |
| ! | TGAGAAAACCTTAATGCTTT+TGG | - | chr7.2:31939113-31939132 | MS.gene42085:intron | 30.0% |
| !! | TGCCTAAAGGTGTTAAATAT+TGG | - | chr7.2:31940065-31940084 | MS.gene42085:intron | 30.0% |
| AATCATATGAGAGGGGAATT+TGG | + | chr7.2:31940117-31940136 | None:intergenic | 35.0% | |
| ACTTGATGTACTTGATGCTA+GGG | - | chr7.2:31938826-31938845 | MS.gene42085:intron | 35.0% | |
| CAACAAGAATCATATGAGAG+GGG | + | chr7.2:31940124-31940143 | None:intergenic | 35.0% | |
| CTCATGAAAAATCCTGCATA+TGG | + | chr7.2:31939310-31939329 | None:intergenic | 35.0% | |
| CTTTCACGGTCACAAATATT+TGG | - | chr7.2:31939185-31939204 | MS.gene42085:intron | 35.0% | |
| ! | ATGCAAGCTCTTTCTTTTGT+TGG | + | chr7.2:31940022-31940041 | None:intergenic | 35.0% |
| ! | ATGGGAAAAGGGTGTAATAA+TGG | + | chr7.2:31939370-31939389 | None:intergenic | 35.0% |
| ! | ATTATTACACCCTTTTCCCA+TGG | - | chr7.2:31939369-31939388 | MS.gene42085:intron | 35.0% |
| ! | CAAGTAATCACGTGAGTTTT+AGG | + | chr7.2:31939729-31939748 | None:intergenic | 35.0% |
| ! | TGGGAAAAGGGTGTAATAAT+GGG | + | chr7.2:31939369-31939388 | None:intergenic | 35.0% |
| !! | GTTGGCTAGTTTATTAGACA+AGG | + | chr7.2:31940004-31940023 | None:intergenic | 35.0% |
| !! | TTTGAGCTCCAAAAGCATTA+AGG | + | chr7.2:31939124-31939143 | None:intergenic | 35.0% |
| !!! | TTAATGCTTTTGGAGCTCAA+AGG | - | chr7.2:31939123-31939142 | MS.gene42085:intron | 35.0% |
| ATATTGGCATTCCCATATGC+AGG | - | chr7.2:31939295-31939314 | MS.gene42085:intron | 40.0% | |
| GACTTGATGTACTTGATGCT+AGG | - | chr7.2:31938825-31938844 | MS.gene42085:intron | 40.0% | |
| GTTGCAAGCAATCAAGAACA+CGG | - | chr7.2:31940208-31940227 | MS.gene42085:CDS | 40.0% | |
| TGGTATGCTGGTATCAATAC+TGG | - | chr7.2:31938784-31938803 | MS.gene42085:CDS | 40.0% | |
| ! | GTATTGATACCAGCATACCA+GGG | + | chr7.2:31938784-31938803 | None:intergenic | 40.0% |
| ! | TTCAAGTGGTTTGTCACCAT+GGG | + | chr7.2:31939388-31939407 | None:intergenic | 40.0% |
| ! | TTTCAAGTGGTTTGTCACCA+TGG | + | chr7.2:31939389-31939408 | None:intergenic | 40.0% |
| !! | ACTGGAAGCTTTTGAAGATC+TGG | - | chr7.2:31938802-31938821 | MS.gene42085:CDS | 40.0% |
| !! | AGTATTGATACCAGCATACC+AGG | + | chr7.2:31938785-31938804 | None:intergenic | 40.0% |
| !! | CTGGAAGCTTTTGAAGATCT+GGG | - | chr7.2:31938803-31938822 | MS.gene42085:CDS | 40.0% |
| !! | CTTTTGGAAAAGAATAGCCC+TGG | - | chr7.2:31938764-31938783 | MS.gene42085:CDS | 40.0% |
| !! | GTGTGAGCGTTGTACAATTT+TGG | - | chr7.2:31939090-31939109 | MS.gene42085:intron | 40.0% |
| AAAGAATAGCCCTGGTATGC+TGG | - | chr7.2:31938772-31938791 | MS.gene42085:CDS | 45.0% | |
| ACTTTCCAGCTAGAAGCTGT+TGG | - | chr7.2:31938863-31938882 | MS.gene42085:intron | 45.0% | |
| CGATAGTATCAATGCGCAAG+TGG | - | chr7.2:31940172-31940191 | MS.gene42085:CDS | 45.0% | |
| GGTTTGTCACCATGGGAAAA+GGG | + | chr7.2:31939381-31939400 | None:intergenic | 45.0% | |
| ! | TGGTTTGTCACCATGGGAAA+AGG | + | chr7.2:31939382-31939401 | None:intergenic | 45.0% |
| !!! | ACGTGAGTTTTAGGTGCTCA+AGG | + | chr7.2:31939720-31939739 | None:intergenic | 45.0% |
| !!! | CGTGAGTTTTAGGTGCTCAA+GGG | + | chr7.2:31939719-31939738 | None:intergenic | 45.0% |
| !! | AATAAATAAAATTTAAAAAG+AGG | + | chr7.2:31939685-31939704 | None:intergenic | 5.0% |
| !!! | TCTTTTTAAATTTTATTTAT+TGG | - | chr7.2:31939684-31939703 | MS.gene42085:intron | 5.0% |
| TTCCAGCTAGAAGCTGTTGG+AGG | - | chr7.2:31938866-31938885 | MS.gene42085:intron | 50.0% | |
| CTCCTCCAACAGCTTCTAGC+TGG | + | chr7.2:31938871-31938890 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 31938752 | 31940244 | 31938752 | ID=MS.gene42085 |
| chr7.2 | mRNA | 31938752 | 31940244 | 31938752 | ID=MS.gene42085.t1;Parent=MS.gene42085 |
| chr7.2 | exon | 31940104 | 31940244 | 31940104 | ID=MS.gene42085.t1.exon1;Parent=MS.gene42085.t1 |
| chr7.2 | CDS | 31940104 | 31940244 | 31940104 | ID=cds.MS.gene42085.t1;Parent=MS.gene42085.t1 |
| chr7.2 | exon | 31938752 | 31938835 | 31938752 | ID=MS.gene42085.t1.exon2;Parent=MS.gene42085.t1 |
| chr7.2 | CDS | 31938752 | 31938835 | 31938752 | ID=cds.MS.gene42085.t1;Parent=MS.gene42085.t1 |
| Gene Sequence |
| Protein sequence |
