Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42107.t1 | XP_003623505.1 | 98 | 151 | 3 | 0 | 1 | 151 | 1 | 151 | 2.50E-84 | 321.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42107.t1 | Q94AK6 | 38.2 | 110 | 59 | 3 | 51 | 151 | 1 | 110 | 1.2e-10 | 67.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42107.t1 | G7KVY7 | 98.0 | 151 | 3 | 0 | 1 | 151 | 1 | 151 | 1.8e-84 | 321.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060320 | MS.gene42107 | 0.817856 | 2.65E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42107.t1 | MTR_7g071760 | 98.013 | 151 | 3 | 0 | 1 | 151 | 1 | 151 | 2.00e-112 | 315 |
| MS.gene42107.t1 | MTR_7g071790 | 98.013 | 151 | 3 | 0 | 1 | 151 | 1 | 151 | 3.47e-112 | 314 |
| MS.gene42107.t1 | MTR_7g071970 | 97.351 | 151 | 4 | 0 | 1 | 151 | 1 | 151 | 3.91e-112 | 314 |
| MS.gene42107.t1 | MTR_7g071840 | 98.013 | 151 | 3 | 0 | 1 | 151 | 1 | 151 | 5.14e-112 | 314 |
| MS.gene42107.t1 | MTR_7g071820 | 96.026 | 151 | 4 | 1 | 1 | 151 | 1 | 149 | 4.91e-107 | 301 |
| MS.gene42107.t1 | MTR_3g031270 | 50.000 | 152 | 65 | 5 | 2 | 151 | 20 | 162 | 1.39e-42 | 139 |
| MS.gene42107.t1 | MTR_3g072030 | 40.132 | 152 | 84 | 3 | 6 | 151 | 50 | 200 | 3.16e-30 | 108 |
| MS.gene42107.t1 | MTR_5g074500 | 37.908 | 153 | 83 | 7 | 6 | 151 | 6 | 153 | 2.37e-22 | 87.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42107.t1 | AT5G01740 | 53.947 | 152 | 60 | 5 | 6 | 151 | 15 | 162 | 6.48e-51 | 160 |
| MS.gene42107.t1 | AT3G10985 | 38.182 | 110 | 59 | 3 | 51 | 151 | 1 | 110 | 2.15e-16 | 70.9 |
Find 35 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGATCTTCCCACTTTAA+TGG | 0.211798 | 7.3:+32750762 | None:intergenic |
| AATTCTCTACAAAGCACTTT+TGG | 0.217894 | 7.3:-32751072 | MS.gene42107:CDS |
| CCAATTGAAGTGACACTTCT+TGG | 0.219369 | 7.3:+32750912 | None:intergenic |
| GTCACTTCCTGGACTTGTTT+TGG | 0.261773 | 7.3:-32750688 | MS.gene42107:CDS |
| GGTGGGAAGGACAAGCTTAT+TGG | 0.263235 | 7.3:-32750869 | MS.gene42107:CDS |
| AAGATCACTTGCTAGCAATT+TGG | 0.274100 | 7.3:+32751025 | None:intergenic |
| GATCTTGAGTATTGGTTTCA+TGG | 0.292356 | 7.3:-32751008 | MS.gene42107:CDS |
| AAGCTTATTGGGTTCATGTT+TGG | 0.330474 | 7.3:-32750857 | MS.gene42107:CDS |
| GTGGGAAGGACAAGCTTATT+GGG | 0.353672 | 7.3:-32750868 | MS.gene42107:CDS |
| TCACCAAAAGATGGACTCTA+TGG | 0.370789 | 7.3:-32751123 | None:intergenic |
| CACATGATGAAGGTGTTAAC+AGG | 0.390291 | 7.3:-32750969 | MS.gene42107:CDS |
| CCAAGAAGTGTCACTTCAAT+TGG | 0.410079 | 7.3:-32750912 | MS.gene42107:CDS |
| CTTTATCGTAGGTCACTTCC+TGG | 0.413885 | 7.3:-32750699 | MS.gene42107:CDS |
| CAATACATGGCTTGTGGTGA+GGG | 0.454357 | 7.3:-32750793 | MS.gene42107:CDS |
| GGGTCAAGGAACAATGGAAA+TGG | 0.460181 | 7.3:-32751051 | MS.gene42107:CDS |
| GGCTTGTGGTGAGGGATTTG+AGG | 0.479336 | 7.3:-32750785 | MS.gene42107:CDS |
| TTTGTAGAGAATTTCAACTA+TGG | 0.506167 | 7.3:+32751082 | None:intergenic |
| TTAGAGAGTATTTCAATACA+TGG | 0.507160 | 7.3:-32750806 | MS.gene42107:CDS |
| TAGCAAGTGATCTTGAGTAT+TGG | 0.519406 | 7.3:-32751016 | MS.gene42107:CDS |
| GGGATTTGAGGCCATTAAAG+TGG | 0.528323 | 7.3:-32750773 | MS.gene42107:CDS |
| TAAATAGCCAAAACAAGTCC+AGG | 0.532508 | 7.3:+32750681 | None:intergenic |
| TTCATCATGTGTTGACATTG+TGG | 0.537051 | 7.3:+32750981 | None:intergenic |
| TCAATACATGGCTTGTGGTG+AGG | 0.547105 | 7.3:-32750794 | MS.gene42107:CDS |
| GGATTTGAGGCCATTAAAGT+GGG | 0.590336 | 7.3:-32750772 | MS.gene42107:CDS |
| ATCATGTGTTGACATTGTGG+TGG | 0.597098 | 7.3:+32750984 | None:intergenic |
| TTGTGTGATAACTGAAGGGT+GGG | 0.598453 | 7.3:-32750886 | MS.gene42107:CDS |
| GTATTTCAATACATGGCTTG+TGG | 0.599070 | 7.3:-32750799 | MS.gene42107:CDS |
| ACAATGTCAACACATGATGA+AGG | 0.602094 | 7.3:-32750979 | MS.gene42107:CDS |
| ATTGTGTGATAACTGAAGGG+TGG | 0.634073 | 7.3:-32750887 | MS.gene42107:CDS |
| GGTGATTGTGTGATAACTGA+AGG | 0.637600 | 7.3:-32750891 | MS.gene42107:CDS |
| AGCAAGATAACATGACATTG+TGG | 0.661544 | 7.3:-32750740 | MS.gene42107:CDS |
| AACCTCGTGATCTTTATCGT+AGG | 0.678620 | 7.3:-32750710 | MS.gene42107:CDS |
| GTGATAACTGAAGGGTGGGA+AGG | 0.682403 | 7.3:-32750882 | MS.gene42107:CDS |
| GTGATTGTGTGATAACTGAA+GGG | 0.691615 | 7.3:-32750890 | MS.gene42107:CDS |
| GACCTACGATAAAGATCACG+AGG | 0.717672 | 7.3:+32750708 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGTTTGGACAATGAAAAAT+GGG | - | chr7.3:32750951-32750970 | MS.gene42107:CDS | 25.0% |
| ! | GAGAAACAAATCACAAAAAA+GGG | - | chr7.3:32750846-32750865 | MS.gene42107:CDS | 25.0% |
| ! | TTTGTAGAGAATTTCAACTA+TGG | + | chr7.3:32750714-32750733 | None:intergenic | 25.0% |
| !!! | TTAGAGAGTATTTCAATACA+TGG | - | chr7.3:32750987-32751006 | MS.gene42107:CDS | 25.0% |
| CATGTTTGGACAATGAAAAA+TGG | - | chr7.3:32750950-32750969 | MS.gene42107:CDS | 30.0% | |
| GGAGAAACAAATCACAAAAA+AGG | - | chr7.3:32750845-32750864 | MS.gene42107:CDS | 30.0% | |
| TTATGATCTTCCCACTTTAA+TGG | + | chr7.3:32751034-32751053 | None:intergenic | 30.0% | |
| !! | AATTCTCTACAAAGCACTTT+TGG | - | chr7.3:32750721-32750740 | MS.gene42107:CDS | 30.0% |
| !! | ATTCTCTACAAAGCACTTTT+GGG | - | chr7.3:32750722-32750741 | MS.gene42107:CDS | 30.0% |
| AAGCTTATTGGGTTCATGTT+TGG | - | chr7.3:32750936-32750955 | MS.gene42107:CDS | 35.0% | |
| ACAATGTCAACACATGATGA+AGG | - | chr7.3:32750814-32750833 | MS.gene42107:CDS | 35.0% | |
| AGCAAGATAACATGACATTG+TGG | - | chr7.3:32751053-32751072 | MS.gene42107:CDS | 35.0% | |
| GTATTTCAATACATGGCTTG+TGG | - | chr7.3:32750994-32751013 | MS.gene42107:CDS | 35.0% | |
| GTGATTGTGTGATAACTGAA+GGG | - | chr7.3:32750903-32750922 | MS.gene42107:CDS | 35.0% | |
| TAAATAGCCAAAACAAGTCC+AGG | + | chr7.3:32751115-32751134 | None:intergenic | 35.0% | |
| TAGCAAGTGATCTTGAGTAT+TGG | - | chr7.3:32750777-32750796 | MS.gene42107:CDS | 35.0% | |
| ! | AAGATCACTTGCTAGCAATT+TGG | + | chr7.3:32750771-32750790 | None:intergenic | 35.0% |
| ! | TTCATCATGTGTTGACATTG+TGG | + | chr7.3:32750815-32750834 | None:intergenic | 35.0% |
| !! | GATCTTGAGTATTGGTTTCA+TGG | - | chr7.3:32750785-32750804 | MS.gene42107:CDS | 35.0% |
| AACCTCGTGATCTTTATCGT+AGG | - | chr7.3:32751083-32751102 | MS.gene42107:CDS | 40.0% | |
| ATTGTGTGATAACTGAAGGG+TGG | - | chr7.3:32750906-32750925 | MS.gene42107:CDS | 40.0% | |
| CCAATTGAAGTGACACTTCT+TGG | + | chr7.3:32750884-32750903 | None:intergenic | 40.0% | |
| GGTGATTGTGTGATAACTGA+AGG | - | chr7.3:32750902-32750921 | MS.gene42107:CDS | 40.0% | |
| TTGTGTGATAACTGAAGGGT+GGG | - | chr7.3:32750907-32750926 | MS.gene42107:CDS | 40.0% | |
| ! | ATCATGTGTTGACATTGTGG+TGG | + | chr7.3:32750812-32750831 | None:intergenic | 40.0% |
| ! | GGATTTGAGGCCATTAAAGT+GGG | - | chr7.3:32751021-32751040 | MS.gene42107:CDS | 40.0% |
| !! | ACTTTTGGGTCAAGGAACAA+TGG | - | chr7.3:32750736-32750755 | MS.gene42107:CDS | 40.0% |
| !! | CACATGATGAAGGTGTTAAC+AGG | - | chr7.3:32750824-32750843 | MS.gene42107:CDS | 40.0% |
| !! | CCAAGAAGTGTCACTTCAAT+TGG | - | chr7.3:32750881-32750900 | MS.gene42107:CDS | 40.0% |
| !! | TACAAAGCACTTTTGGGTCA+AGG | - | chr7.3:32750728-32750747 | MS.gene42107:CDS | 40.0% |
| CTTTATCGTAGGTCACTTCC+TGG | - | chr7.3:32751094-32751113 | MS.gene42107:CDS | 45.0% | |
| GACCTACGATAAAGATCACG+AGG | + | chr7.3:32751088-32751107 | None:intergenic | 45.0% | |
| GGGTCAAGGAACAATGGAAA+TGG | - | chr7.3:32750742-32750761 | MS.gene42107:CDS | 45.0% | |
| GTGGGAAGGACAAGCTTATT+GGG | - | chr7.3:32750925-32750944 | MS.gene42107:CDS | 45.0% | |
| ! | CAATACATGGCTTGTGGTGA+GGG | - | chr7.3:32751000-32751019 | MS.gene42107:CDS | 45.0% |
| ! | GGGATTTGAGGCCATTAAAG+TGG | - | chr7.3:32751020-32751039 | MS.gene42107:CDS | 45.0% |
| ! | TCAATACATGGCTTGTGGTG+AGG | - | chr7.3:32750999-32751018 | MS.gene42107:CDS | 45.0% |
| !! | GTCACTTCCTGGACTTGTTT+TGG | - | chr7.3:32751105-32751124 | MS.gene42107:CDS | 45.0% |
| GGTGGGAAGGACAAGCTTAT+TGG | - | chr7.3:32750924-32750943 | MS.gene42107:CDS | 50.0% | |
| GTGATAACTGAAGGGTGGGA+AGG | - | chr7.3:32750911-32750930 | MS.gene42107:CDS | 50.0% | |
| GGCTTGTGGTGAGGGATTTG+AGG | - | chr7.3:32751008-32751027 | MS.gene42107:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 32750680 | 32751135 | 32750680 | ID=MS.gene42107 |
| chr7.3 | mRNA | 32750680 | 32751135 | 32750680 | ID=MS.gene42107.t1;Parent=MS.gene42107 |
| chr7.3 | exon | 32750680 | 32751135 | 32750680 | ID=MS.gene42107.t1.exon1;Parent=MS.gene42107.t1 |
| chr7.3 | CDS | 32750680 | 32751135 | 32750680 | ID=cds.MS.gene42107.t1;Parent=MS.gene42107.t1 |
| Gene Sequence |
| Protein sequence |
