Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42148.t1 | XP_003623411.2 | 96.9 | 451 | 13 | 1 | 1 | 451 | 3 | 452 | 3.80E-245 | 857.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42148.t1 | Q9SCU1 | 72.8 | 400 | 103 | 3 | 36 | 435 | 11 | 404 | 6.7e-166 | 585.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42148.t1 | G7KU47 | 96.8 | 437 | 13 | 1 | 15 | 451 | 1 | 436 | 1.0e-236 | 828.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049697 | MS.gene42148 | 0.800086 | 1.70E-48 | -1.69E-46 |
| MS.gene052383 | MS.gene42148 | 0.802289 | 6.02E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene42148 | 0.826828 | 2.19E-54 | -1.69E-46 |
| MS.gene054999 | MS.gene42148 | 0.813672 | 2.27E-51 | -1.69E-46 |
| MS.gene056528 | MS.gene42148 | 0.800049 | 1.73E-48 | -1.69E-46 |
| MS.gene056618 | MS.gene42148 | 0.814401 | 1.57E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42148.t1 | MTR_7g070480 | 96.796 | 437 | 13 | 1 | 15 | 451 | 1 | 436 | 0.0 | 870 |
| MS.gene42148.t1 | MTR_4g013300 | 71.236 | 445 | 117 | 5 | 1 | 435 | 3 | 446 | 0.0 | 647 |
| MS.gene42148.t1 | MTR_8g095360 | 43.571 | 420 | 218 | 12 | 13 | 426 | 85 | 491 | 7.03e-114 | 344 |
| MS.gene42148.t1 | MTR_2g031610 | 42.488 | 426 | 190 | 9 | 59 | 435 | 46 | 465 | 3.21e-111 | 336 |
| MS.gene42148.t1 | MTR_4g033435 | 48.071 | 337 | 164 | 8 | 99 | 430 | 257 | 587 | 2.21e-105 | 325 |
| MS.gene42148.t1 | MTR_3g097350 | 48.328 | 329 | 159 | 8 | 98 | 421 | 234 | 556 | 1.55e-104 | 322 |
| MS.gene42148.t1 | MTR_1g069915 | 38.905 | 347 | 200 | 7 | 85 | 426 | 118 | 457 | 1.02e-76 | 247 |
| MS.gene42148.t1 | MTR_4g033435 | 51.887 | 212 | 94 | 5 | 99 | 306 | 257 | 464 | 3.72e-70 | 230 |
| MS.gene42148.t1 | MTR_8g096170 | 27.854 | 219 | 142 | 5 | 112 | 328 | 50 | 254 | 2.58e-18 | 84.7 |
| MS.gene42148.t1 | MTR_3g098530 | 26.415 | 265 | 166 | 7 | 70 | 319 | 12 | 262 | 3.22e-18 | 84.7 |
| MS.gene42148.t1 | MTR_5g021050 | 27.068 | 266 | 159 | 9 | 74 | 325 | 48 | 292 | 4.31e-18 | 86.7 |
| MS.gene42148.t1 | MTR_5g021050 | 27.068 | 266 | 159 | 9 | 74 | 325 | 48 | 292 | 5.58e-18 | 86.7 |
| MS.gene42148.t1 | MTR_8g096180 | 28.095 | 210 | 135 | 5 | 112 | 319 | 47 | 242 | 1.48e-17 | 82.4 |
| MS.gene42148.t1 | MTR_3g117100 | 25.974 | 308 | 193 | 11 | 40 | 325 | 32 | 326 | 5.30e-17 | 84.0 |
| MS.gene42148.t1 | MTR_8g096180 | 26.941 | 219 | 136 | 5 | 112 | 319 | 47 | 252 | 9.67e-16 | 77.4 |
| MS.gene42148.t1 | MTR_4g070810 | 27.928 | 222 | 133 | 8 | 114 | 319 | 107 | 317 | 2.30e-15 | 79.0 |
| MS.gene42148.t1 | MTR_1g022480 | 26.364 | 220 | 139 | 8 | 114 | 319 | 112 | 322 | 9.40e-15 | 77.0 |
| MS.gene42148.t1 | MTR_1g022480 | 26.364 | 220 | 139 | 8 | 114 | 319 | 112 | 322 | 1.03e-14 | 76.6 |
| MS.gene42148.t1 | MTR_1g022480 | 26.364 | 220 | 139 | 8 | 114 | 319 | 112 | 322 | 1.16e-14 | 76.6 |
| MS.gene42148.t1 | MTR_4g070790 | 26.818 | 220 | 138 | 7 | 114 | 319 | 84 | 294 | 1.22e-14 | 75.9 |
| MS.gene42148.t1 | MTR_1g062480 | 52.542 | 59 | 19 | 1 | 275 | 333 | 43 | 92 | 1.57e-14 | 69.7 |
| MS.gene42148.t1 | MTR_4g070720 | 25.079 | 315 | 182 | 11 | 114 | 395 | 96 | 389 | 1.80e-14 | 75.9 |
| MS.gene42148.t1 | MTR_3g112150 | 27.602 | 221 | 135 | 8 | 114 | 319 | 93 | 303 | 2.15e-14 | 75.9 |
| MS.gene42148.t1 | MTR_3g117090 | 26.667 | 225 | 144 | 6 | 114 | 325 | 97 | 313 | 2.70e-14 | 75.5 |
| MS.gene42148.t1 | MTR_3g117090 | 26.667 | 225 | 144 | 6 | 114 | 325 | 97 | 313 | 2.82e-14 | 75.5 |
| MS.gene42148.t1 | MTR_1g022480 | 25.806 | 217 | 138 | 8 | 117 | 319 | 31 | 238 | 2.28e-13 | 72.4 |
| MS.gene42148.t1 | MTR_8g076880 | 27.434 | 226 | 141 | 7 | 114 | 325 | 79 | 295 | 1.06e-12 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42148.t1 | AT3G51670 | 72.750 | 400 | 103 | 3 | 36 | 435 | 11 | 404 | 0.0 | 605 |
| MS.gene42148.t1 | AT1G72160 | 46.512 | 387 | 188 | 12 | 61 | 434 | 108 | 488 | 7.79e-110 | 333 |
| MS.gene42148.t1 | AT4G09160 | 47.520 | 383 | 183 | 11 | 61 | 434 | 265 | 638 | 4.25e-109 | 336 |
| MS.gene42148.t1 | AT4G09160 | 47.520 | 383 | 183 | 11 | 61 | 434 | 265 | 638 | 5.26e-109 | 336 |
| MS.gene42148.t1 | AT4G09160 | 47.520 | 383 | 183 | 11 | 61 | 434 | 290 | 663 | 6.52e-109 | 336 |
| MS.gene42148.t1 | AT1G30690 | 44.540 | 348 | 174 | 8 | 99 | 439 | 205 | 540 | 8.68e-96 | 298 |
| MS.gene42148.t1 | AT1G30690 | 44.540 | 348 | 174 | 8 | 99 | 439 | 205 | 540 | 8.68e-96 | 298 |
| MS.gene42148.t1 | AT1G22530 | 43.452 | 336 | 171 | 9 | 98 | 426 | 318 | 641 | 1.10e-84 | 273 |
| MS.gene42148.t1 | AT1G22530 | 43.155 | 336 | 172 | 9 | 98 | 426 | 352 | 675 | 1.46e-84 | 273 |
| MS.gene42148.t1 | AT1G72150 | 35.335 | 433 | 249 | 12 | 10 | 431 | 158 | 570 | 3.94e-77 | 251 |
| MS.gene42148.t1 | AT2G21540 | 28.319 | 226 | 139 | 7 | 114 | 325 | 95 | 311 | 3.31e-17 | 84.3 |
| MS.gene42148.t1 | AT2G21540 | 28.319 | 226 | 139 | 7 | 114 | 325 | 95 | 311 | 3.31e-17 | 84.3 |
| MS.gene42148.t1 | AT2G21540 | 28.319 | 226 | 139 | 7 | 114 | 325 | 95 | 311 | 3.31e-17 | 84.3 |
| MS.gene42148.t1 | AT2G21540 | 28.319 | 226 | 139 | 7 | 114 | 325 | 95 | 311 | 3.31e-17 | 84.3 |
| MS.gene42148.t1 | AT2G21540 | 28.319 | 226 | 139 | 7 | 114 | 325 | 95 | 311 | 3.51e-17 | 84.3 |
| MS.gene42148.t1 | AT2G21540 | 28.319 | 226 | 139 | 7 | 114 | 325 | 95 | 311 | 3.51e-17 | 84.3 |
| MS.gene42148.t1 | AT4G39180 | 28.319 | 226 | 139 | 7 | 114 | 325 | 96 | 312 | 5.63e-17 | 84.0 |
| MS.gene42148.t1 | AT4G39180 | 28.319 | 226 | 139 | 7 | 114 | 325 | 96 | 312 | 5.63e-17 | 84.0 |
| MS.gene42148.t1 | AT4G39180 | 28.319 | 226 | 139 | 7 | 114 | 325 | 96 | 312 | 5.90e-17 | 83.6 |
| MS.gene42148.t1 | AT4G39170 | 26.266 | 316 | 187 | 13 | 40 | 329 | 32 | 327 | 2.81e-16 | 81.6 |
| MS.gene42148.t1 | AT4G39170 | 26.266 | 316 | 187 | 13 | 40 | 329 | 32 | 327 | 2.85e-16 | 81.6 |
| MS.gene42148.t1 | AT4G34580 | 27.753 | 227 | 139 | 8 | 114 | 325 | 88 | 304 | 4.04e-16 | 81.3 |
| MS.gene42148.t1 | AT4G34580 | 27.753 | 227 | 139 | 8 | 114 | 325 | 88 | 304 | 4.04e-16 | 81.3 |
| MS.gene42148.t1 | AT4G36490 | 27.434 | 226 | 141 | 7 | 114 | 325 | 78 | 294 | 7.25e-16 | 80.5 |
| MS.gene42148.t1 | AT2G18180 | 28.319 | 226 | 139 | 7 | 114 | 325 | 81 | 297 | 1.87e-15 | 79.0 |
| MS.gene42148.t1 | AT2G18180 | 28.319 | 226 | 139 | 7 | 114 | 325 | 81 | 297 | 2.06e-15 | 79.0 |
| MS.gene42148.t1 | AT1G19650 | 28.017 | 232 | 139 | 9 | 114 | 329 | 104 | 323 | 3.43e-15 | 78.2 |
| MS.gene42148.t1 | AT2G16380 | 26.599 | 297 | 174 | 12 | 60 | 325 | 21 | 304 | 4.09e-15 | 77.8 |
| MS.gene42148.t1 | AT2G16380 | 26.599 | 297 | 174 | 12 | 60 | 325 | 21 | 304 | 4.09e-15 | 77.8 |
| MS.gene42148.t1 | AT1G19650 | 28.017 | 232 | 139 | 9 | 114 | 329 | 104 | 323 | 4.45e-15 | 77.4 |
| MS.gene42148.t1 | AT2G18180 | 28.000 | 225 | 139 | 7 | 115 | 325 | 11 | 226 | 1.81e-14 | 75.9 |
| MS.gene42148.t1 | AT2G21520 | 24.275 | 276 | 177 | 9 | 40 | 294 | 32 | 296 | 2.00e-14 | 75.9 |
| MS.gene42148.t1 | AT2G21520 | 24.275 | 276 | 177 | 9 | 40 | 294 | 36 | 300 | 2.12e-14 | 75.9 |
| MS.gene42148.t1 | AT2G21520 | 24.275 | 276 | 177 | 9 | 40 | 294 | 36 | 300 | 2.12e-14 | 75.9 |
| MS.gene42148.t1 | AT2G18180 | 28.000 | 225 | 139 | 7 | 115 | 325 | 11 | 226 | 2.43e-14 | 75.5 |
| MS.gene42148.t1 | AT1G01630 | 26.389 | 216 | 137 | 6 | 109 | 319 | 47 | 245 | 1.52e-13 | 70.9 |
| MS.gene42148.t1 | AT4G08690 | 23.832 | 214 | 143 | 5 | 110 | 319 | 41 | 238 | 2.85e-13 | 70.9 |
| MS.gene42148.t1 | AT4G08690 | 23.832 | 214 | 143 | 5 | 110 | 319 | 41 | 238 | 2.85e-13 | 70.9 |
| MS.gene42148.t1 | AT4G08690 | 23.832 | 214 | 143 | 5 | 110 | 319 | 41 | 238 | 2.85e-13 | 70.9 |
| MS.gene42148.t1 | AT3G24840 | 27.273 | 220 | 135 | 8 | 115 | 319 | 104 | 313 | 9.92e-13 | 70.5 |
| MS.gene42148.t1 | AT3G24840 | 27.273 | 220 | 135 | 8 | 115 | 319 | 104 | 313 | 9.92e-13 | 70.5 |
| MS.gene42148.t1 | AT3G24840 | 27.273 | 220 | 135 | 8 | 115 | 319 | 104 | 313 | 9.92e-13 | 70.5 |
| MS.gene42148.t1 | AT3G24840 | 27.273 | 220 | 135 | 8 | 115 | 319 | 104 | 313 | 1.01e-12 | 70.5 |
| MS.gene42148.t1 | AT3G24840 | 27.273 | 220 | 135 | 8 | 115 | 319 | 104 | 313 | 1.01e-12 | 70.5 |
| MS.gene42148.t1 | AT3G24840 | 27.273 | 220 | 135 | 8 | 115 | 319 | 104 | 313 | 1.01e-12 | 70.5 |
| MS.gene42148.t1 | AT3G24840 | 27.273 | 220 | 135 | 8 | 115 | 319 | 104 | 313 | 1.01e-12 | 70.5 |
| MS.gene42148.t1 | AT1G55840 | 25.463 | 216 | 130 | 8 | 115 | 314 | 39 | 239 | 2.21e-12 | 68.2 |
| MS.gene42148.t1 | AT1G55840 | 25.463 | 216 | 130 | 8 | 115 | 314 | 12 | 212 | 2.27e-12 | 68.2 |
| MS.gene42148.t1 | AT1G75170 | 25.822 | 213 | 141 | 5 | 110 | 319 | 42 | 240 | 8.24e-11 | 63.2 |
| MS.gene42148.t1 | AT1G75170 | 25.822 | 213 | 141 | 5 | 110 | 319 | 42 | 240 | 8.24e-11 | 63.2 |
Find 74 sgRNAs with CRISPR-Local
Find 147 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTCTGAGTTCACTGTTAA+AGG | 0.184177 | 7.3:-34248200 | MS.gene42148:CDS |
| GTGAACTCAGAAGCTGGTTT+TGG | 0.239830 | 7.3:+34248209 | None:intergenic |
| TTCCATTAATGTTGTTACTA+TGG | 0.244429 | 7.3:+34249660 | None:intergenic |
| AACCATAGTAACAACATTAA+TGG | 0.252522 | 7.3:-34249662 | MS.gene42148:CDS |
| TTTGAGGTTGGAAATGAAAT+AGG | 0.258263 | 7.3:+34249600 | None:intergenic |
| ACCTTACGAGCAACCATTTC+AGG | 0.266158 | 7.3:+34249025 | None:intergenic |
| CTTTCTTGGAGGAAAGAATT+TGG | 0.299122 | 7.3:-34249388 | MS.gene42148:CDS |
| TGCTTGTGAAGTGTCTTTCT+TGG | 0.302342 | 7.3:-34249402 | MS.gene42148:CDS |
| AAATGTTGTGGATGAAGATT+TGG | 0.311161 | 7.3:-34249359 | MS.gene42148:CDS |
| TGTAGTTGGAGGCTGGGATT+TGG | 0.312615 | 7.3:-34247435 | MS.gene42148:CDS |
| CGGTCGATAACTCTGCTTCT+AGG | 0.324064 | 7.3:-34247277 | MS.gene42148:CDS |
| GTGTAGCTAGCTTCTGCATT+TGG | 0.328696 | 7.3:+34247392 | None:intergenic |
| GATTCAAGTTACTGATCTTA+AGG | 0.332587 | 7.3:-34249113 | MS.gene42148:CDS |
| GAGAATATTCCTATTCAATA+TGG | 0.337092 | 7.3:-34248269 | MS.gene42148:CDS |
| CTTTCTTCTCAGAGGATTTG+AGG | 0.350173 | 7.3:+34249584 | None:intergenic |
| TTCCATGCAGAAATTTCATC+TGG | 0.363571 | 7.3:-34249806 | None:intergenic |
| TTAGTTCTTTGAGTCAGAAA+TGG | 0.368588 | 7.3:+34248586 | None:intergenic |
| TTTCTTGGAGTTTCTAGAAA+TGG | 0.388074 | 7.3:+34249709 | None:intergenic |
| GAGCAAGTTTGTGATCTCTA+AGG | 0.402946 | 7.3:-34248563 | MS.gene42148:CDS |
| TCAAGATAACTATCCTGAAA+TGG | 0.404693 | 7.3:-34249038 | MS.gene42148:CDS |
| GGGAGATTGTAGTTGGAGGC+TGG | 0.407967 | 7.3:-34247442 | MS.gene42148:CDS |
| ACAGCCCTTAACTCACTCTT+AGG | 0.419674 | 7.3:+34249085 | None:intergenic |
| AATTCCTCATTCACCATCAA+TGG | 0.429468 | 7.3:-34249773 | MS.gene42148:CDS |
| GAGTGATCTTCTTCCATTGA+TGG | 0.440321 | 7.3:+34249760 | None:intergenic |
| AATGTTGTGGATGAAGATTT+GGG | 0.445253 | 7.3:-34249358 | MS.gene42148:CDS |
| ATCCCACATGGAACCATTGT+TGG | 0.450891 | 7.3:+34249516 | None:intergenic |
| CTTCTCAGAGGATTTGAGGT+TGG | 0.453013 | 7.3:+34249588 | None:intergenic |
| CTCAATCCACCATATTGAAT+AGG | 0.453387 | 7.3:+34248260 | None:intergenic |
| TCCTGAAATGGTTGCTCGTA+AGG | 0.456947 | 7.3:-34249026 | MS.gene42148:intron |
| GGAGATTGTAGTTGGAGGCT+GGG | 0.463853 | 7.3:-34247441 | MS.gene42148:CDS |
| ATATGCCTAAGAGTGAGTTA+AGG | 0.470060 | 7.3:-34249090 | MS.gene42148:CDS |
| AAAGTGAACATACAAATAGA+AGG | 0.473146 | 7.3:-34248170 | MS.gene42148:CDS |
| TCTTCCATTGATGGTGAATG+AGG | 0.473222 | 7.3:+34249769 | None:intergenic |
| TATGCCTAAGAGTGAGTTAA+GGG | 0.484189 | 7.3:-34249089 | MS.gene42148:CDS |
| ATCAAAGAATTCACCCCACC+AGG | 0.485860 | 7.3:+34249133 | None:intergenic |
| CTACACTATAGAAGTTGAAA+AGG | 0.488723 | 7.3:-34247375 | MS.gene42148:CDS |
| CCTGTGTGTTACAATCATTA+TGG | 0.491385 | 7.3:-34249277 | MS.gene42148:CDS |
| TTGTGAAGTGTCTTTCTTGG+AGG | 0.498038 | 7.3:-34249399 | MS.gene42148:CDS |
| ATCAAGTGGGAGATTGTAGT+TGG | 0.499306 | 7.3:-34247449 | MS.gene42148:CDS |
| GAAAGCATTGAAACAGTTGA+AGG | 0.500192 | 7.3:-34249566 | MS.gene42148:CDS |
| TTATGGTGTGTTCAAAGATA+AGG | 0.503248 | 7.3:-34249260 | MS.gene42148:CDS |
| TACTCATGGGTTTGATAGAG+AGG | 0.507545 | 7.3:-34249305 | MS.gene42148:CDS |
| TTGGGATTCAAAGAGCTTGA+AGG | 0.513195 | 7.3:-34249340 | MS.gene42148:CDS |
| TTAACAGTGAACTCAGAAGC+TGG | 0.523617 | 7.3:+34248203 | None:intergenic |
| AATATTCCTATTCAATATGG+TGG | 0.536024 | 7.3:-34248266 | MS.gene42148:CDS |
| AGGCTTAATTGCATATCAGA+TGG | 0.543533 | 7.3:+34247200 | None:intergenic |
| AGCAGGCATAATAGTACTCT+CGG | 0.546809 | 7.3:-34247297 | MS.gene42148:CDS |
| TTTACAGTTTAAACCTGGTG+GGG | 0.553174 | 7.3:-34249146 | MS.gene42148:CDS |
| TAAGCCTCAAAATCAAATAG+TGG | 0.553201 | 7.3:-34247184 | None:intergenic |
| TCTGATGAAATTACCAACAA+TGG | 0.557226 | 7.3:-34249529 | MS.gene42148:CDS |
| TATTGAATAGGAATATTCTC+AGG | 0.562576 | 7.3:+34248272 | None:intergenic |
| TCATTCACAACAAAAGAAGC+AGG | 0.565896 | 7.3:-34247314 | MS.gene42148:CDS |
| ACTCATGGGTTTGATAGAGA+GGG | 0.567192 | 7.3:-34249304 | MS.gene42148:CDS |
| ATTTGGTGCTGAAAATGTTG+TGG | 0.567986 | 7.3:-34249371 | MS.gene42148:CDS |
| GGGTTCAAGTTCTTGAGAGA+GGG | 0.569611 | 7.3:-34249177 | MS.gene42148:CDS |
| TCCATGCAGAAATTTCATCT+GGG | 0.581919 | 7.3:-34249805 | None:intergenic |
| TTTCAATGCTTTCTTCTCAG+AGG | 0.582552 | 7.3:+34249576 | None:intergenic |
| AAGTGGGAGATTGTAGTTGG+AGG | 0.586468 | 7.3:-34247446 | MS.gene42148:CDS |
| AGATAAGGAAATGTATGAGA+AGG | 0.595028 | 7.3:-34249245 | MS.gene42148:CDS |
| CCATAATGATTGTAACACAC+AGG | 0.599317 | 7.3:+34249277 | None:intergenic |
| CTATAGAAGTTGAAAAGGCA+AGG | 0.600332 | 7.3:-34247370 | MS.gene42148:CDS |
| AGGGTTCAAGTTCTTGAGAG+AGG | 0.610104 | 7.3:-34249178 | MS.gene42148:CDS |
| CATACAAATAGAAGGAGTTG+AGG | 0.610399 | 7.3:-34248162 | MS.gene42148:intron |
| TACAACATGCTGAAATACCA+TGG | 0.625322 | 7.3:+34248622 | None:intergenic |
| AGAGTGGTGCAACAATCAAG+TGG | 0.626220 | 7.3:-34247463 | MS.gene42148:CDS |
| GGACCATTCTGAAAATCACT+AGG | 0.626931 | 7.3:+34248233 | None:intergenic |
| TACCAACAATGGTTCCATGT+GGG | 0.630135 | 7.3:-34249518 | MS.gene42148:CDS |
| TCTGAGTTCACTGTTAAAGG+AGG | 0.633144 | 7.3:-34248197 | MS.gene42148:CDS |
| AAGTTTGTGATCTCTAAGGA+AGG | 0.634705 | 7.3:-34248559 | MS.gene42148:CDS |
| TGGGATGTTAGTTTGATCAA+AGG | 0.641687 | 7.3:-34249499 | MS.gene42148:CDS |
| GATCAAACTAACATCCCACA+TGG | 0.655540 | 7.3:+34249504 | None:intergenic |
| GAGTGGTGCAACAATCAAGT+GGG | 0.657297 | 7.3:-34247462 | MS.gene42148:CDS |
| CATAATGATTGTAACACACA+GGG | 0.659937 | 7.3:+34249278 | None:intergenic |
| TTACCAACAATGGTTCCATG+TGG | 0.689918 | 7.3:-34249519 | MS.gene42148:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAATTACAGTGAAATAT+TGG | - | chr7.3:34248188-34248207 | MS.gene42148:CDS | 15.0% |
| !!! | AAATTTAACTACATTTTACA+CGG | - | chr7.3:34248935-34248954 | MS.gene42148:intron | 15.0% |
| !!! | TTTTTTTGTTCAGATTTATA+AGG | - | chr7.3:34248688-34248707 | MS.gene42148:intron | 15.0% |
| !! | AAGTATGAGTATATCAAAAT+TGG | + | chr7.3:34249223-34249242 | None:intergenic | 20.0% |
| !! | TCTTATTAGAGACTATATAA+TGG | - | chr7.3:34248869-34248888 | MS.gene42148:intron | 20.0% |
| !! | TTTCATGTCATACTAAATAT+AGG | + | chr7.3:34248305-34248324 | None:intergenic | 20.0% |
| !!! | AATTTTGATATACTCATACT+TGG | - | chr7.3:34249222-34249241 | MS.gene42148:CDS | 20.0% |
| !!! | AGATGTAATTTTAGAAAATC+TGG | - | chr7.3:34248507-34248526 | MS.gene42148:intron | 20.0% |
| !!! | ATTTTGATATACTCATACTT+GGG | - | chr7.3:34249223-34249242 | MS.gene42148:CDS | 20.0% |
| !!! | CATGTATTGTTTTGTTATTT+TGG | - | chr7.3:34248042-34248061 | MS.gene42148:intron | 20.0% |
| !!! | TTATTTTGGTGTTGTTTATT+TGG | - | chr7.3:34248056-34248075 | MS.gene42148:intron | 20.0% |
| ! | AAAGTGAACATACAAATAGA+AGG | - | chr7.3:34248813-34248832 | MS.gene42148:intron | 25.0% |
| ! | AAATCTTATACTAAGTGTCA+CGG | + | chr7.3:34249283-34249302 | None:intergenic | 25.0% |
| ! | AACCATAGTAACAACATTAA+TGG | - | chr7.3:34247321-34247340 | MS.gene42148:CDS | 25.0% |
| ! | AATATTCCTATTCAATATGG+TGG | - | chr7.3:34248717-34248736 | MS.gene42148:intron | 25.0% |
| ! | ACAAAAACAATGTCATATCA+AGG | + | chr7.3:34247985-34248004 | None:intergenic | 25.0% |
| ! | ACAATAATAGAAACATTGTG+GGG | + | chr7.3:34248645-34248664 | None:intergenic | 25.0% |
| ! | CACAATAATAGAAACATTGT+GGG | + | chr7.3:34248646-34248665 | None:intergenic | 25.0% |
| ! | GACTATATAATGGTTACTTA+TGG | - | chr7.3:34248879-34248898 | MS.gene42148:intron | 25.0% |
| ! | GAGAATATTCCTATTCAATA+TGG | - | chr7.3:34248714-34248733 | MS.gene42148:intron | 25.0% |
| ! | GTAGTTAACGAAATGAAATT+CGG | + | chr7.3:34249028-34249047 | None:intergenic | 25.0% |
| ! | TAATGGTTACTTATGGTTTA+AGG | - | chr7.3:34248886-34248905 | MS.gene42148:intron | 25.0% |
| ! | TATTGAATAGGAATATTCTC+AGG | + | chr7.3:34248714-34248733 | None:intergenic | 25.0% |
| ! | TTAATTATATGTGTGAGTGT+CGG | + | chr7.3:34248174-34248193 | None:intergenic | 25.0% |
| ! | TTCCATTAATGTTGTTACTA+TGG | + | chr7.3:34247326-34247345 | None:intergenic | 25.0% |
| !! | AAAAGTTGAAGAAGTTTTTG+AGG | - | chr7.3:34247779-34247798 | MS.gene42148:intron | 25.0% |
| !! | CATAAACATCATTGATGTTT+TGG | + | chr7.3:34248480-34248499 | None:intergenic | 25.0% |
| !! | GTGTATTGTTACTGTATTTT+CGG | + | chr7.3:34249764-34249783 | None:intergenic | 25.0% |
| !! | TTTTGATATACTCATACTTG+GGG | - | chr7.3:34249224-34249243 | MS.gene42148:CDS | 25.0% |
| !!! | ACGTTTGATTTTTGTGTTAA+CGG | - | chr7.3:34249386-34249405 | MS.gene42148:CDS | 25.0% |
| !!! | ATTTTGTTTGATACATAGAC+AGG | + | chr7.3:34248612-34248631 | None:intergenic | 25.0% |
| AAATGTTGTGGATGAAGATT+TGG | - | chr7.3:34247624-34247643 | MS.gene42148:intron | 30.0% | |
| AATGTTGTGGATGAAGATTT+GGG | - | chr7.3:34247625-34247644 | MS.gene42148:intron | 30.0% | |
| ACACTTAGTATAAGATTTCC+AGG | - | chr7.3:34249285-34249304 | MS.gene42148:CDS | 30.0% | |
| AGATAAGGAAATGTATGAGA+AGG | - | chr7.3:34247738-34247757 | MS.gene42148:intron | 30.0% | |
| AGCAAAAGTAAAGCCTAAAA+AGG | + | chr7.3:34248581-34248600 | None:intergenic | 30.0% | |
| CATAATGATTGTAACACACA+GGG | + | chr7.3:34247708-34247727 | None:intergenic | 30.0% | |
| CTACACTATAGAAGTTGAAA+AGG | - | chr7.3:34249608-34249627 | MS.gene42148:CDS | 30.0% | |
| GCACAATAATAGAAACATTG+TGG | + | chr7.3:34248647-34248666 | None:intergenic | 30.0% | |
| GTAGCAAATTGCTTTAACAT+TGG | + | chr7.3:34249456-34249475 | None:intergenic | 30.0% | |
| GTTTGTAACGTTCAAATTTG+TGG | - | chr7.3:34249119-34249138 | MS.gene42148:CDS | 30.0% | |
| TCAAGATAACTATCCTGAAA+TGG | - | chr7.3:34247945-34247964 | MS.gene42148:intron | 30.0% | |
| TCTCAAATTGTTAACAGTGT+CGG | - | chr7.3:34248215-34248234 | MS.gene42148:CDS | 30.0% | |
| TCTGATGAAATTACCAACAA+TGG | - | chr7.3:34247454-34247473 | MS.gene42148:CDS | 30.0% | |
| TTATGGTGTGTTCAAAGATA+AGG | - | chr7.3:34247723-34247742 | MS.gene42148:intron | 30.0% | |
| ! | AAGCTTTTACAGTTTAAACC+TGG | - | chr7.3:34247832-34247851 | MS.gene42148:intron | 30.0% |
| ! | ACAGTTGAAACATCTGAAAT+CGG | - | chr7.3:34249095-34249114 | MS.gene42148:CDS | 30.0% |
| ! | GAAATGTATGAGAAGGTTTT+TGG | - | chr7.3:34247745-34247764 | MS.gene42148:intron | 30.0% |
| ! | GATTCAAGTTACTGATCTTA+AGG | - | chr7.3:34247870-34247889 | MS.gene42148:intron | 30.0% |
| ! | GTTACTATGGTTCTTTTGTT+TGG | + | chr7.3:34247313-34247332 | None:intergenic | 30.0% |
| ! | TTAGTTCTTTGAGTCAGAAA+TGG | + | chr7.3:34248400-34248419 | None:intergenic | 30.0% |
| ! | TTTCTTGGAGTTTCTAGAAA+TGG | + | chr7.3:34247277-34247296 | None:intergenic | 30.0% |
| ! | TTTGAGGTTGGAAATGAAAT+AGG | + | chr7.3:34247386-34247405 | None:intergenic | 30.0% |
| ! | TTTGTGACTGTTTCCTTTTT+AGG | - | chr7.3:34248565-34248584 | MS.gene42148:CDS | 30.0% |
| ! | TTTTACAGTTTAAACCTGGT+GGG | - | chr7.3:34247836-34247855 | MS.gene42148:intron | 30.0% |
| !! | CGTGATTAGTCACAATTTTT+AGG | - | chr7.3:34249057-34249076 | MS.gene42148:CDS | 30.0% |
| !!! | GTTCTTTTGTTTGGTTTTGA+GGG | + | chr7.3:34247304-34247323 | None:intergenic | 30.0% |
| AACTCGCAAACAAATATGCT+CGG | - | chr7.3:34249315-34249334 | MS.gene42148:CDS | 35.0% | |
| AAGTGTCACAGTTCATTAAC+CGG | - | chr7.3:34248999-34249018 | MS.gene42148:intron | 35.0% | |
| AATTCCTCATTCACCATCAA+TGG | - | chr7.3:34247210-34247229 | MS.gene42148:CDS | 35.0% | |
| AGGCTTAATTGCATATCAGA+TGG | + | chr7.3:34249786-34249805 | None:intergenic | 35.0% | |
| ATATGCCTAAGAGTGAGTTA+AGG | - | chr7.3:34247893-34247912 | MS.gene42148:intron | 35.0% | |
| CATACAAATAGAAGGAGTTG+AGG | - | chr7.3:34248821-34248840 | MS.gene42148:intron | 35.0% | |
| CCATAATGATTGTAACACAC+AGG | + | chr7.3:34247709-34247728 | None:intergenic | 35.0% | |
| CCTGTGTGTTACAATCATTA+TGG | - | chr7.3:34247706-34247725 | MS.gene42148:intron | 35.0% | |
| CTATAGAAGTTGAAAAGGCA+AGG | - | chr7.3:34249613-34249632 | MS.gene42148:CDS | 35.0% | |
| CTCAATCCACCATATTGAAT+AGG | + | chr7.3:34248726-34248745 | None:intergenic | 35.0% | |
| CTTTCTTGGAGGAAAGAATT+TGG | - | chr7.3:34247595-34247614 | MS.gene42148:intron | 35.0% | |
| TAAAATTGACTACCTAGCGA+CGG | + | chr7.3:34249259-34249278 | None:intergenic | 35.0% | |
| TACAACATGCTGAAATACCA+TGG | + | chr7.3:34248364-34248383 | None:intergenic | 35.0% | |
| TATGCCTAAGAGTGAGTTAA+GGG | - | chr7.3:34247894-34247913 | MS.gene42148:intron | 35.0% | |
| TCATTCACAACAAAAGAAGC+AGG | - | chr7.3:34249669-34249688 | MS.gene42148:CDS | 35.0% | |
| TTACACCCAATATTGAAGCA+CGG | - | chr7.3:34248105-34248124 | MS.gene42148:intron | 35.0% | |
| TTTACAGTTTAAACCTGGTG+GGG | - | chr7.3:34247837-34247856 | MS.gene42148:intron | 35.0% | |
| TTTCAATGCTTTCTTCTCAG+AGG | + | chr7.3:34247410-34247429 | None:intergenic | 35.0% | |
| ! | AAGTTTGTGATCTCTAAGGA+AGG | - | chr7.3:34248424-34248443 | MS.gene42148:intron | 35.0% |
| ! | AGATTTTCATCAATGTGCCA+TGG | - | chr7.3:34248344-34248363 | MS.gene42148:intron | 35.0% |
| ! | CTTTTACAGTTTAAACCTGG+TGG | - | chr7.3:34247835-34247854 | MS.gene42148:intron | 35.0% |
| ! | TCACTATTTGCTGATTAGAG+TGG | - | chr7.3:34249504-34249523 | MS.gene42148:CDS | 35.0% |
| ! | TGGGATGTTAGTTTGATCAA+AGG | - | chr7.3:34247484-34247503 | MS.gene42148:intron | 35.0% |
| !! | AGGCTTTACTTTTGCTGAAT+TGG | - | chr7.3:34248585-34248604 | MS.gene42148:CDS | 35.0% |
| !! | AGTTGAAGAAGTTTTTGAGG+TGG | - | chr7.3:34247782-34247801 | MS.gene42148:intron | 35.0% |
| !! | ATTTGGTGCTGAAAATGTTG+TGG | - | chr7.3:34247612-34247631 | MS.gene42148:intron | 35.0% |
| !! | GAAAGCATTGAAACAGTTGA+AGG | - | chr7.3:34247417-34247436 | MS.gene42148:CDS | 35.0% |
| !!! | AGGTTTTGAGTTCGAACTTT+TGG | - | chr7.3:34248906-34248925 | MS.gene42148:intron | 35.0% |
| !!! | GGTTCTTTTGTTTGGTTTTG+AGG | + | chr7.3:34247305-34247324 | None:intergenic | 35.0% |
| !!! | GTGTTGTTGCTTTTACTCAT+GGG | - | chr7.3:34247665-34247684 | MS.gene42148:intron | 35.0% |
| AATATTGAAGCACGGACACT+CGG | - | chr7.3:34248113-34248132 | MS.gene42148:intron | 40.0% | |
| ACGAAATGAAATTCGGTAGC+CGG | + | chr7.3:34249021-34249040 | None:intergenic | 40.0% | |
| ACTCATGGGTTTGATAGAGA+GGG | - | chr7.3:34247679-34247698 | MS.gene42148:intron | 40.0% | |
| AGCAGGCATAATAGTACTCT+CGG | - | chr7.3:34249686-34249705 | MS.gene42148:CDS | 40.0% | |
| AGTGTCCGTGCTTCAATATT+GGG | + | chr7.3:34248113-34248132 | None:intergenic | 40.0% | |
| ATCAAGTGGGAGATTGTAGT+TGG | - | chr7.3:34249534-34249553 | MS.gene42148:CDS | 40.0% | |
| CTTTCTTCTCAGAGGATTTG+AGG | + | chr7.3:34247402-34247421 | None:intergenic | 40.0% | |
| GAGCAAGTTTGTGATCTCTA+AGG | - | chr7.3:34248420-34248439 | MS.gene42148:intron | 40.0% | |
| GAGTGATCTTCTTCCATTGA+TGG | + | chr7.3:34247226-34247245 | None:intergenic | 40.0% | |
| GATCAAACTAACATCCCACA+TGG | + | chr7.3:34247482-34247501 | None:intergenic | 40.0% | |
| GCTTCTGAGTTCACTGTTAA+AGG | - | chr7.3:34248783-34248802 | MS.gene42148:intron | 40.0% | |
| TACCAACAATGGTTCCATGT+GGG | - | chr7.3:34247465-34247484 | MS.gene42148:intron | 40.0% | |
| TACTCATGGGTTTGATAGAG+AGG | - | chr7.3:34247678-34247697 | MS.gene42148:intron | 40.0% | |
| TCTGAGTTCACTGTTAAAGG+AGG | - | chr7.3:34248786-34248805 | MS.gene42148:intron | 40.0% | |
| TTAACAGTGAACTCAGAAGC+TGG | + | chr7.3:34248783-34248802 | None:intergenic | 40.0% | |
| TTACCAACAATGGTTCCATG+TGG | - | chr7.3:34247464-34247483 | MS.gene42148:CDS | 40.0% | |
| TTGGGATTCAAAGAGCTTGA+AGG | - | chr7.3:34247643-34247662 | MS.gene42148:intron | 40.0% | |
| ! | CGACCTAGTGATTTTCAGAA+TGG | - | chr7.3:34248747-34248766 | MS.gene42148:intron | 40.0% |
| ! | GGACCATTCTGAAAATCACT+AGG | + | chr7.3:34248753-34248772 | None:intergenic | 40.0% |
| ! | TGCTTGTGAAGTGTCTTTCT+TGG | - | chr7.3:34247581-34247600 | MS.gene42148:intron | 40.0% |
| ! | TTGTGAAGTGTCTTTCTTGG+AGG | - | chr7.3:34247584-34247603 | MS.gene42148:intron | 40.0% |
| !! | TCTTCCATTGATGGTGAATG+AGG | + | chr7.3:34247217-34247236 | None:intergenic | 40.0% |
| !!! | GAAGAAGTTTTTGAGGTGGA+GGG | - | chr7.3:34247786-34247805 | MS.gene42148:intron | 40.0% |
| !!! | GGTGTTGTTGCTTTTACTCA+TGG | - | chr7.3:34247664-34247683 | MS.gene42148:intron | 40.0% |
| !!! | GGTTTTGAGGGAGTTTTTCT+TGG | + | chr7.3:34247292-34247311 | None:intergenic | 40.0% |
| !!! | TGAACTCAGAAGCTGGTTTT+GGG | + | chr7.3:34248776-34248795 | None:intergenic | 40.0% |
| !!! | TGAAGAAGTTTTTGAGGTGG+AGG | - | chr7.3:34247785-34247804 | MS.gene42148:intron | 40.0% |
| AAGTGGGAGATTGTAGTTGG+AGG | - | chr7.3:34249537-34249556 | MS.gene42148:CDS | 45.0% | |
| ACAGCCCTTAACTCACTCTT+AGG | + | chr7.3:34247901-34247920 | None:intergenic | 45.0% | |
| ACCTTACGAGCAACCATTTC+AGG | + | chr7.3:34247961-34247980 | None:intergenic | 45.0% | |
| AGAGTGGTGCAACAATCAAG+TGG | - | chr7.3:34249520-34249539 | MS.gene42148:CDS | 45.0% | |
| ATCAAAGAATTCACCCCACC+AGG | + | chr7.3:34247853-34247872 | None:intergenic | 45.0% | |
| ATCAAGCACGGATACGAACA+CGG | + | chr7.3:34248272-34248291 | None:intergenic | 45.0% | |
| ATCCCACATGGAACCATTGT+TGG | + | chr7.3:34247470-34247489 | None:intergenic | 45.0% | |
| GAGTGGTGCAACAATCAAGT+GGG | - | chr7.3:34249521-34249540 | MS.gene42148:CDS | 45.0% | |
| GAGTGTCCGTGCTTCAATAT+TGG | + | chr7.3:34248114-34248133 | None:intergenic | 45.0% | |
| GGTGTAAGTCCTATCAAGCA+CGG | + | chr7.3:34248284-34248303 | None:intergenic | 45.0% | |
| GGTGTGTTAGTATCCGTGTT+CGG | - | chr7.3:34248236-34248255 | MS.gene42148:CDS | 45.0% | |
| GTTTGCGAGTTTCTTGATCC+TGG | + | chr7.3:34249306-34249325 | None:intergenic | 45.0% | |
| TCCTGAAATGGTTGCTCGTA+AGG | - | chr7.3:34247957-34247976 | MS.gene42148:intron | 45.0% | |
| TGTTCGTATCCGTGCTTGAT+AGG | - | chr7.3:34248272-34248291 | MS.gene42148:CDS | 45.0% | |
| ! | AGGGTTCAAGTTCTTGAGAG+AGG | - | chr7.3:34247805-34247824 | MS.gene42148:intron | 45.0% |
| ! | CTTCTCAGAGGATTTGAGGT+TGG | + | chr7.3:34247398-34247417 | None:intergenic | 45.0% |
| ! | GGGTTCAAGTTCTTGAGAGA+GGG | - | chr7.3:34247806-34247825 | MS.gene42148:intron | 45.0% |
| ! | GTGTAGCTAGCTTCTGCATT+TGG | + | chr7.3:34249594-34249613 | None:intergenic | 45.0% |
| !! | GTGAACTCAGAAGCTGGTTT+TGG | + | chr7.3:34248777-34248796 | None:intergenic | 45.0% |
| !!! | AACTCAGAAGCTGGTTTTGG+GGG | + | chr7.3:34248774-34248793 | None:intergenic | 45.0% |
| !!! | GAACTCAGAAGCTGGTTTTG+GGG | + | chr7.3:34248775-34248794 | None:intergenic | 45.0% |
| ATACGACATGTGTCCGACAC+TGG | + | chr7.3:34248151-34248170 | None:intergenic | 50.0% | |
| CGCAAACAAATATGCTCGGC+TGG | - | chr7.3:34249319-34249338 | MS.gene42148:CDS | 50.0% | |
| CGGTCGATAACTCTGCTTCT+AGG | - | chr7.3:34249706-34249725 | MS.gene42148:CDS | 50.0% | |
| GATCAGCTGTGTTCCAGTGT+CGG | - | chr7.3:34248135-34248154 | MS.gene42148:intron | 50.0% | |
| GGAGATTGTAGTTGGAGGCT+GGG | - | chr7.3:34249542-34249561 | MS.gene42148:CDS | 50.0% | |
| TGTAGTTGGAGGCTGGGATT+TGG | - | chr7.3:34249548-34249567 | MS.gene42148:CDS | 50.0% | |
| !! | TAGTATCCGTGTTCGGTGTC+CGG | - | chr7.3:34248243-34248262 | MS.gene42148:CDS | 50.0% |
| ACGGATACGAACACGGACAC+CGG | + | chr7.3:34248265-34248284 | None:intergenic | 55.0% | |
| GGGAGATTGTAGTTGGAGGC+TGG | - | chr7.3:34249541-34249560 | MS.gene42148:CDS | 55.0% | |
| TTGACTACCTAGCGACGGAG+TGG | + | chr7.3:34249254-34249273 | None:intergenic | 55.0% | |
| CGGACACCGGACACCGAACA+CGG | + | chr7.3:34248252-34248271 | None:intergenic | 65.0% | |
| GGGTCATCCACTCCGTCGCT+AGG | - | chr7.3:34249244-34249263 | MS.gene42148:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 34247187 | 34249818 | 34247187 | ID=MS.gene42148 |
| chr7.3 | mRNA | 34247187 | 34249818 | 34247187 | ID=MS.gene42148.t1;Parent=MS.gene42148 |
| chr7.3 | exon | 34249027 | 34249818 | 34249027 | ID=MS.gene42148.t1.exon1;Parent=MS.gene42148.t1 |
| chr7.3 | CDS | 34249027 | 34249818 | 34249027 | ID=cds.MS.gene42148.t1;Parent=MS.gene42148.t1 |
| chr7.3 | exon | 34248535 | 34248659 | 34248535 | ID=MS.gene42148.t1.exon2;Parent=MS.gene42148.t1 |
| chr7.3 | CDS | 34248535 | 34248659 | 34248535 | ID=cds.MS.gene42148.t1;Parent=MS.gene42148.t1 |
| chr7.3 | exon | 34248163 | 34248304 | 34248163 | ID=MS.gene42148.t1.exon3;Parent=MS.gene42148.t1 |
| chr7.3 | CDS | 34248163 | 34248304 | 34248163 | ID=cds.MS.gene42148.t1;Parent=MS.gene42148.t1 |
| chr7.3 | exon | 34247187 | 34247483 | 34247187 | ID=MS.gene42148.t1.exon4;Parent=MS.gene42148.t1 |
| chr7.3 | CDS | 34247187 | 34247483 | 34247187 | ID=cds.MS.gene42148.t1;Parent=MS.gene42148.t1 |
| Gene Sequence |
| Protein sequence |
