Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054614.t1 | XP_003602994.2 | 97.6 | 1001 | 23 | 1 | 1 | 1000 | 1 | 1001 | 0.00E+00 | 1944.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054614.t1 | Q8BKN5 | 28.9 | 360 | 198 | 8 | 631 | 965 | 687 | 1013 | 4.5e-29 | 131.7 |
| MS.gene054614.t1 | Q8BKN5 | 22.5 | 347 | 222 | 8 | 24 | 353 | 255 | 571 | 5.5e-11 | 71.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054614.t1 | G7J5S9 | 97.6 | 1001 | 23 | 1 | 1 | 1000 | 1 | 1001 | 0.0e+00 | 1944.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049660 | MS.gene054614 | 0.819268 | 1.27E-52 | -1.69E-46 |
| MS.gene05054 | MS.gene054614 | 0.813503 | 2.48E-51 | -1.69E-46 |
| MS.gene052367 | MS.gene054614 | 0.809634 | 1.72E-50 | -1.69E-46 |
| MS.gene052383 | MS.gene054614 | 0.83726 | 5.77E-57 | -1.69E-46 |
| MS.gene053348 | MS.gene054614 | 0.80895 | 2.41E-50 | -1.69E-46 |
| MS.gene054551 | MS.gene054614 | 0.800244 | 1.58E-48 | -1.69E-46 |
| MS.gene054614 | MS.gene058382 | 0.807091 | 5.99E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene058853 | 0.810519 | 1.11E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene061275 | 0.806378 | 8.47E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene061357 | 0.84373 | 1.17E-58 | -1.69E-46 |
| MS.gene054614 | MS.gene061366 | 0.830925 | 2.23E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene061775 | 0.809439 | 1.89E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene063035 | 0.829422 | 5.19E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene063037 | 0.850144 | 2.05E-60 | -1.69E-46 |
| MS.gene054614 | MS.gene063180 | 0.801296 | 9.63E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene065292 | 0.807467 | 4.98E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene065643 | 0.829547 | 4.84E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene066026 | 0.800933 | 1.14E-48 | -1.69E-46 |
| MS.gene054614 | MS.gene066030 | -0.801185 | 1.01E-48 | -1.69E-46 |
| MS.gene054614 | MS.gene066265 | 0.844342 | 8.02E-59 | -1.69E-46 |
| MS.gene054614 | MS.gene067074 | 0.80296 | 4.38E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene068190 | 0.816341 | 5.81E-52 | -1.69E-46 |
| MS.gene054614 | MS.gene069090 | 0.831113 | 2.01E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene07138 | 0.840218 | 9.92E-58 | -1.69E-46 |
| MS.gene054614 | MS.gene071428 | 0.833646 | 4.73E-56 | -1.69E-46 |
| MS.gene054614 | MS.gene072777 | 0.801015 | 1.10E-48 | -1.69E-46 |
| MS.gene054614 | MS.gene07618 | 0.837253 | 5.80E-57 | -1.69E-46 |
| MS.gene054614 | MS.gene07620 | 0.838086 | 3.54E-57 | -1.69E-46 |
| MS.gene054614 | MS.gene07622 | 0.842187 | 3.01E-58 | -1.69E-46 |
| MS.gene054614 | MS.gene09356 | 0.834249 | 3.34E-56 | -1.69E-46 |
| MS.gene054614 | MS.gene09801 | 0.83134 | 1.76E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene20032 | 0.833714 | 4.55E-56 | -1.69E-46 |
| MS.gene054614 | MS.gene20986 | 0.82119 | 4.61E-53 | -1.69E-46 |
| MS.gene054614 | MS.gene21055 | 0.813642 | 2.31E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene22383 | 0.828606 | 8.19E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene23003 | 0.828082 | 1.10E-54 | -1.69E-46 |
| MS.gene054614 | MS.gene23421 | 0.839751 | 1.31E-57 | -1.69E-46 |
| MS.gene054614 | MS.gene24224 | 0.840579 | 7.99E-58 | -1.69E-46 |
| MS.gene054614 | MS.gene24738 | 0.801488 | 8.79E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene25797 | 0.800594 | 1.34E-48 | -1.69E-46 |
| MS.gene054614 | MS.gene25932 | 0.816861 | 4.45E-52 | -1.69E-46 |
| MS.gene054614 | MS.gene26665 | 0.835492 | 1.63E-56 | -1.69E-46 |
| MS.gene054614 | MS.gene27011 | 0.842705 | 2.20E-58 | -1.69E-46 |
| MS.gene054614 | MS.gene27811 | 0.814726 | 1.33E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene27812 | 0.816352 | 5.78E-52 | -1.69E-46 |
| MS.gene054614 | MS.gene29215 | 0.814402 | 1.57E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene31917 | 0.817784 | 2.76E-52 | -1.69E-46 |
| MS.gene054614 | MS.gene32638 | 0.801742 | 7.80E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene33809 | 0.837771 | 4.27E-57 | -1.69E-46 |
| MS.gene054614 | MS.gene37573 | 0.81308 | 3.07E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene38688 | 0.80172 | 7.88E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene40749 | 0.812275 | 4.60E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene41273 | 0.813715 | 2.22E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene42070 | 0.8075 | 4.90E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene42147 | 0.817096 | 3.94E-52 | -1.69E-46 |
| MS.gene054614 | MS.gene42148 | 0.826828 | 2.19E-54 | -1.69E-46 |
| MS.gene054614 | MS.gene42510 | 0.804181 | 2.44E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene43866 | 0.804773 | 1.84E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene44073 | 0.818942 | 1.51E-52 | -1.69E-46 |
| MS.gene054614 | MS.gene47366 | 0.813032 | 3.14E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene49165 | 0.829074 | 6.31E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene49744 | 0.880555 | 4.98E-70 | -1.69E-46 |
| MS.gene054614 | MS.gene52607 | 0.832684 | 8.22E-56 | -1.69E-46 |
| MS.gene054614 | MS.gene52976 | -0.805109 | 1.56E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene53408 | 0.814515 | 1.48E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene54855 | 0.832108 | 1.14E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene57289 | 0.820308 | 7.35E-53 | -1.69E-46 |
| MS.gene054614 | MS.gene59357 | 0.839029 | 2.02E-57 | -1.69E-46 |
| MS.gene054614 | MS.gene61081 | 0.803391 | 3.56E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene63265 | 0.822005 | 2.99E-53 | -1.69E-46 |
| MS.gene054614 | MS.gene64442 | 0.859338 | 4.43E-63 | -1.69E-46 |
| MS.gene054614 | MS.gene68090 | 0.806798 | 6.91E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene68280 | 0.815573 | 8.63E-52 | -1.69E-46 |
| MS.gene054614 | MS.gene70141 | 0.862124 | 6.33E-64 | -1.69E-46 |
| MS.gene054614 | MS.gene70403 | 0.837322 | 5.56E-57 | -1.69E-46 |
| MS.gene054614 | MS.gene70604 | 0.812739 | 3.64E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene71420 | -0.801717 | 7.89E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene71431 | 0.814681 | 1.36E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene72866 | 0.800898 | 1.16E-48 | -1.69E-46 |
| MS.gene054614 | MS.gene74328 | 0.81251 | 4.09E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene74352 | 0.807226 | 5.61E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene74358 | 0.80193 | 7.14E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene76883 | 0.801802 | 7.58E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene77771 | 0.832017 | 1.20E-55 | -1.69E-46 |
| MS.gene054614 | MS.gene77774 | 0.801025 | 1.09E-48 | -1.69E-46 |
| MS.gene054614 | MS.gene78065 | 0.807106 | 5.94E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene83358 | 0.841518 | 4.53E-58 | -1.69E-46 |
| MS.gene054614 | MS.gene87690 | 0.801723 | 7.87E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene88616 | 0.801502 | 8.74E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene90732 | 0.822342 | 2.49E-53 | -1.69E-46 |
| MS.gene054614 | MS.gene90876 | 0.807132 | 5.87E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene90984 | 0.850851 | 1.30E-60 | -1.69E-46 |
| MS.gene054614 | MS.gene91133 | 0.849289 | 3.56E-60 | -1.69E-46 |
| MS.gene054614 | MS.gene91527 | 0.815635 | 8.36E-52 | -1.69E-46 |
| MS.gene054614 | MS.gene91781 | 0.824822 | 6.55E-54 | -1.69E-46 |
| MS.gene054614 | MS.gene93738 | -0.803068 | 4.16E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene95532 | 0.826545 | 2.56E-54 | -1.69E-46 |
| MS.gene054614 | MS.gene96390 | 0.807335 | 5.32E-50 | -1.69E-46 |
| MS.gene054614 | MS.gene96758 | 0.803352 | 3.63E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene96836 | 0.824888 | 6.32E-54 | -1.69E-46 |
| MS.gene054614 | MS.gene99127 | 0.801229 | 9.94E-49 | -1.69E-46 |
| MS.gene054614 | MS.gene99603 | 0.817338 | 3.47E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054614.t1 | MTR_3g101230 | 97.602 | 1001 | 23 | 1 | 1 | 1000 | 1 | 1001 | 0.0 | 2031 |
| MS.gene054614.t1 | MTR_5g004880 | 24.543 | 383 | 244 | 14 | 621 | 983 | 825 | 1182 | 2.66e-13 | 75.1 |
| MS.gene054614.t1 | MTR_5g004880 | 24.740 | 384 | 243 | 15 | 621 | 983 | 825 | 1183 | 1.32e-12 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054614.t1 | AT1G80260 | 57.708 | 986 | 370 | 12 | 29 | 994 | 29 | 987 | 0.0 | 1074 |
| MS.gene054614.t1 | AT1G20570 | 55.692 | 975 | 390 | 15 | 30 | 987 | 35 | 984 | 0.0 | 1001 |
| MS.gene054614.t1 | AT1G20570 | 55.487 | 975 | 390 | 16 | 30 | 987 | 35 | 982 | 0.0 | 991 |
| MS.gene054614.t1 | AT1G20570 | 54.872 | 975 | 389 | 17 | 30 | 987 | 35 | 975 | 0.0 | 974 |
| MS.gene054614.t1 | AT1G80260 | 53.737 | 843 | 343 | 12 | 29 | 851 | 29 | 844 | 0.0 | 830 |
| MS.gene054614.t1 | AT1G80260 | 52.671 | 805 | 334 | 12 | 29 | 813 | 29 | 806 | 0.0 | 766 |
| MS.gene054614.t1 | AT1G20570 | 48.052 | 693 | 318 | 14 | 30 | 707 | 35 | 700 | 0.0 | 572 |
| MS.gene054614.t1 | AT3G43610 | 24.834 | 302 | 190 | 9 | 623 | 908 | 841 | 1121 | 1.19e-13 | 76.3 |
| MS.gene054614.t1 | AT3G43610 | 24.834 | 302 | 190 | 9 | 623 | 908 | 804 | 1084 | 1.33e-13 | 75.9 |
| MS.gene054614.t1 | AT3G43610 | 24.752 | 303 | 190 | 10 | 623 | 908 | 841 | 1122 | 1.04e-12 | 73.2 |
Find 197 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTAATGTCTTCATATTT+TGG | 0.108022 | 3.4:-89133081 | None:intergenic |
| TCTTTGTCTAGTACAATTAA+AGG | 0.202650 | 3.4:+89132607 | MS.gene054614:CDS |
| AAAATAGTTGTAGTTAATTC+TGG | 0.245536 | 3.4:-89139691 | None:intergenic |
| ATCCTGTGAGTGAATGAATT+TGG | 0.247200 | 3.4:-89133367 | None:intergenic |
| CCAAAATATGAAGACATTAA+TGG | 0.249934 | 3.4:+89133081 | MS.gene054614:CDS |
| GGTTTAAGAAAGGCTATATT+TGG | 0.254968 | 3.4:+89133276 | MS.gene054614:CDS |
| TTTCCCTCATTTGGCTGATT+TGG | 0.256083 | 3.4:+89139665 | MS.gene054614:CDS |
| TAGCACGTCTGGTTCTGTTT+TGG | 0.261887 | 3.4:+89136429 | MS.gene054614:CDS |
| GTATCACTAGCTGGACTTAT+TGG | 0.265996 | 3.4:+89132859 | MS.gene054614:CDS |
| CAGTTACTGAGGCTGAGTTC+TGG | 0.268258 | 3.4:+89132455 | MS.gene054614:CDS |
| CAGTGAATTACCAGTTTATC+AGG | 0.287087 | 3.4:-89138964 | None:intergenic |
| ATATTGAGGGTCTTGATTCT+TGG | 0.289056 | 3.4:+89131877 | MS.gene054614:CDS |
| CAGACCATAGTTTAAAGATT+TGG | 0.290102 | 3.4:-89132788 | None:intergenic |
| AGCTTGTCCTTTGTTTATAA+AGG | 0.298700 | 3.4:+89132657 | MS.gene054614:CDS |
| TTCTGCTTCTTCGTGGCTTA+AGG | 0.310879 | 3.4:+89130947 | MS.gene054614:CDS |
| AGAGGAAGTAATGTTAAGTT+TGG | 0.314974 | 3.4:+89132760 | MS.gene054614:CDS |
| GTTAAGTGCTCCAGATTCTT+TGG | 0.323705 | 3.4:+89136363 | MS.gene054614:CDS |
| CACCTTACTCAGAGAAGATT+TGG | 0.325152 | 3.4:+89133007 | MS.gene054614:CDS |
| TGAATTAAACACAATATTAC+AGG | 0.330031 | 3.4:+89134701 | MS.gene054614:CDS |
| ATTGCATTGCTTTCTTACTT+AGG | 0.332429 | 3.4:+89139243 | MS.gene054614:CDS |
| TGTAGGGCATTTCCCTCATT+TGG | 0.334632 | 3.4:+89139656 | MS.gene054614:CDS |
| GAATCAAGACCCTCAATATA+TGG | 0.343580 | 3.4:-89131873 | None:intergenic |
| TCAAGATTGGGGAAAGCCTC+TGG | 0.345846 | 3.4:+89139768 | MS.gene054614:CDS |
| GAAGGAACAAACTATACTTT+TGG | 0.361584 | 3.4:+89133327 | MS.gene054614:CDS |
| TTTCCTCTTCCTTATTCTCA+TGG | 0.362612 | 3.4:+89130736 | MS.gene054614:CDS |
| AATCTTGAAGCAACACTTCC+CGG | 0.362939 | 3.4:-89139753 | None:intergenic |
| GGAAGTGTTGCTTCAAGATT+GGG | 0.363487 | 3.4:+89139756 | MS.gene054614:CDS |
| CTACATGCTGCTAAGGAACT+TGG | 0.364026 | 3.4:-89132736 | None:intergenic |
| GCTAAGTCAATTATTTCTGC+TGG | 0.366977 | 3.4:+89132685 | MS.gene054614:CDS |
| AGTACCAAATCTTTAAACTA+TGG | 0.368250 | 3.4:+89132784 | MS.gene054614:CDS |
| ATTGAAACTACCAAAGAATC+TGG | 0.368302 | 3.4:-89136373 | None:intergenic |
| GCAATATACTTGTTAGGTTC+AGG | 0.369764 | 3.4:+89133840 | MS.gene054614:CDS |
| CATCAAACTGTTTCTCAATT+CGG | 0.371950 | 3.4:-89139220 | None:intergenic |
| TCCTTCTTCTCATTTGATGC+TGG | 0.373994 | 3.4:-89132562 | None:intergenic |
| AAGGAAGAGTGCTGTTTCTC+TGG | 0.378596 | 3.4:-89133562 | None:intergenic |
| TATGCTAAAATTCACATATA+AGG | 0.379494 | 3.4:+89136498 | MS.gene054614:CDS |
| CTGCGAGCAATATACTTGTT+AGG | 0.380884 | 3.4:+89133834 | MS.gene054614:CDS |
| ATAGAACAGGTAGATTATAT+TGG | 0.382368 | 3.4:+89133753 | MS.gene054614:intron |
| TACCTGAACTGCAGGCAAAA+TGG | 0.384045 | 3.4:-89133427 | None:intergenic |
| CTTTGTGGTTCCTGATAAAC+TGG | 0.387709 | 3.4:+89138954 | MS.gene054614:CDS |
| GGGAAGTGTTGCTTCAAGAT+TGG | 0.394963 | 3.4:+89139755 | MS.gene054614:CDS |
| AATGACCTCATCCAGAGATT+TGG | 0.397636 | 3.4:-89138890 | None:intergenic |
| ATCTGTACCTGCAGCAGACT+TGG | 0.399383 | 3.4:+89131644 | MS.gene054614:CDS |
| TTGCATTGCTTTCTTACTTA+GGG | 0.400349 | 3.4:+89139244 | MS.gene054614:CDS |
| TTATCTTTCAAACTAAATGT+AGG | 0.403353 | 3.4:+89139639 | MS.gene054614:CDS |
| ATCAAATGAGAAGAAGGAAA+TGG | 0.406917 | 3.4:+89132567 | MS.gene054614:CDS |
| GTTGAGGAATGTTGCGTTGA+AGG | 0.407731 | 3.4:+89131070 | MS.gene054614:CDS |
| TTTCGACAAACGGTAATAAC+GGG | 0.415411 | 3.4:-89133174 | None:intergenic |
| GTTGTGATTCCATGAGAATA+AGG | 0.419331 | 3.4:-89130745 | None:intergenic |
| AGTTACTGAGGCTGAGTTCT+GGG | 0.419762 | 3.4:+89132456 | MS.gene054614:CDS |
| CCAAACTCAAAATATACATC+AGG | 0.420178 | 3.4:-89131618 | None:intergenic |
| CTTCTCATTTGATGCTGGCA+TGG | 0.420448 | 3.4:-89132557 | None:intergenic |
| CTCCTTCTCATAGAGTGGTT+GGG | 0.422938 | 3.4:+89132812 | MS.gene054614:CDS |
| TCTCCTTCTCATAGAGTGGT+TGG | 0.427105 | 3.4:+89132811 | MS.gene054614:CDS |
| AACTGCCTACATTCAGAAAC+AGG | 0.429486 | 3.4:+89133661 | MS.gene054614:CDS |
| TATGACAGTGGCCAAATCTC+TGG | 0.432406 | 3.4:+89138879 | MS.gene054614:CDS |
| CTCCGTTATTGATACGAAAA+CGG | 0.435968 | 3.4:-89130773 | None:intergenic |
| AGAAGGAAGAGGAGTTGCGA+AGG | 0.436418 | 3.4:-89130658 | None:intergenic |
| AAAATATTGTTGAGAGTGAT+GGG | 0.443508 | 3.4:+89136401 | MS.gene054614:CDS |
| CCTCATGAACTATTCGCAAT+AGG | 0.443607 | 3.4:-89131590 | None:intergenic |
| TGTTGACTTAGTCTTTGCTC+TGG | 0.443814 | 3.4:+89131558 | MS.gene054614:intron |
| TAAAAGTCGTGTCAATAACT+TGG | 0.444724 | 3.4:+89136459 | MS.gene054614:CDS |
| TTCCTTCAGAAGTTCTTCAC+TGG | 0.445367 | 3.4:+89133579 | MS.gene054614:CDS |
| AACGAACTTGAACTCGTTCG+AGG | 0.445931 | 3.4:+89130690 | MS.gene054614:CDS |
| AGCCGCGTTAATATCATCCT+TGG | 0.446699 | 3.4:+89139107 | MS.gene054614:CDS |
| CGGAGCTGTTTCTGCAATCA+AGG | 0.447886 | 3.4:+89139175 | MS.gene054614:CDS |
| AATACTTCTGCCAAGATGAT+TGG | 0.449525 | 3.4:+89132902 | MS.gene054614:CDS |
| ATTAATTACAGGAATCAATC+AGG | 0.450032 | 3.4:+89136320 | MS.gene054614:intron |
| GATGGGGAAGCTAGCACGTC+TGG | 0.454223 | 3.4:+89136418 | MS.gene054614:CDS |
| CAGATTCATGCCAATCATCT+TGG | 0.456196 | 3.4:-89132912 | None:intergenic |
| AACGCATTCAAAGTAGGATT+AGG | 0.457958 | 3.4:-89130918 | None:intergenic |
| TTGTTGCTTCTGCTTCTTCG+TGG | 0.458339 | 3.4:+89130940 | MS.gene054614:CDS |
| TTCACCAATATGTGATGGAC+CGG | 0.459217 | 3.4:+89137138 | MS.gene054614:CDS |
| AAATGACGAGGGTTTAAGAA+AGG | 0.459804 | 3.4:+89133266 | MS.gene054614:CDS |
| GGGAATTTAATGACCACATC+TGG | 0.462590 | 3.4:+89139729 | MS.gene054614:CDS |
| TACCAAATCTTCTCTGAGTA+AGG | 0.465054 | 3.4:-89133009 | None:intergenic |
| AATTCAGCGGCAGTGCTTTG+TGG | 0.465133 | 3.4:+89138939 | MS.gene054614:CDS |
| AAAAGTCGTGTCAATAACTT+GGG | 0.470045 | 3.4:+89136460 | MS.gene054614:CDS |
| ATAAAGTGCGGAGATGGATG+TGG | 0.470423 | 3.4:+89136883 | MS.gene054614:CDS |
| AAGCTTGTTGCCATATATTG+AGG | 0.472101 | 3.4:+89131863 | MS.gene054614:CDS |
| TACATGCTGCTAAGGAACTT+GGG | 0.475663 | 3.4:-89132735 | None:intergenic |
| GCAGGCAAAATGGTGGGAAA+AGG | 0.481625 | 3.4:-89133417 | None:intergenic |
| TTAAGAAAGGCTATATTTGG+TGG | 0.484839 | 3.4:+89133279 | MS.gene054614:CDS |
| CAAATAACAGAAAACATCAT+TGG | 0.485901 | 3.4:+89137078 | MS.gene054614:CDS |
| CTTGCCTTCTTTAAATGACG+AGG | 0.487749 | 3.4:+89133254 | MS.gene054614:CDS |
| TTTGGAACTGGCAGGTACCT+TGG | 0.489890 | 3.4:+89136617 | MS.gene054614:intron |
| ATTCACCTGTTTCTGAATGT+AGG | 0.490395 | 3.4:-89133666 | None:intergenic |
| TCAAATGAGAAGAAGGAAAT+GGG | 0.491559 | 3.4:+89132568 | MS.gene054614:CDS |
| TATTAGATAAAGTGCGGAGA+TGG | 0.494973 | 3.4:+89136877 | MS.gene054614:CDS |
| GAGGTAGTGGAGTCGTTCTT+AGG | 0.495595 | 3.4:-89133616 | None:intergenic |
| AAACCCTCGTCATTTAAAGA+AGG | 0.496105 | 3.4:-89133258 | None:intergenic |
| TTTAATGACCACATCTGGCC+CGG | 0.497421 | 3.4:+89139734 | MS.gene054614:CDS |
| AAGGGGAATTCGTTGGAGAA+TGG | 0.502014 | 3.4:-89130639 | None:intergenic |
| GAAGGAAGAGGAGTTGCGAA+GGG | 0.503176 | 3.4:-89130657 | None:intergenic |
| TCCTCTGCTACATGCTGCTA+AGG | 0.506439 | 3.4:-89132743 | None:intergenic |
| TTGTGGTGATGCAGCGGTTG+AGG | 0.507310 | 3.4:+89131054 | MS.gene054614:intron |
| TGCATAATAACCAGAGGTAG+TGG | 0.507745 | 3.4:-89133629 | None:intergenic |
| GCCAGCATCAAATGAGAAGA+AGG | 0.508393 | 3.4:+89132561 | MS.gene054614:CDS |
| TATCTTTCAAACTAAATGTA+GGG | 0.510323 | 3.4:+89139640 | MS.gene054614:CDS |
| CATGGTACTTTACATGTATG+TGG | 0.511026 | 3.4:+89131839 | MS.gene054614:CDS |
| GTCAATAACTTGGGGATGAA+CGG | 0.512497 | 3.4:+89136469 | MS.gene054614:CDS |
| TTTATAAACAAAGGACAAGC+TGG | 0.514362 | 3.4:-89132655 | None:intergenic |
| TGTGGATTAAGGTTTGGGGA+TGG | 0.515687 | 3.4:-89130605 | None:intergenic |
| TGTTAAAGTTGATGAATGAA+TGG | 0.519572 | 3.4:+89133787 | MS.gene054614:CDS |
| ATACCCGGTCCATCACATAT+TGG | 0.522988 | 3.4:-89137142 | None:intergenic |
| CAGAAAACATCATTGGTTAG+TGG | 0.526266 | 3.4:+89137085 | MS.gene054614:CDS |
| GATGAATGAATGGAGGTTGA+TGG | 0.526491 | 3.4:+89133797 | MS.gene054614:CDS |
| AAGGATGATATTAACGCGGC+TGG | 0.528179 | 3.4:-89139105 | None:intergenic |
| AGCTTGTTGCCATATATTGA+GGG | 0.528688 | 3.4:+89131864 | MS.gene054614:CDS |
| GAAGAAGGAAATGGGCATGA+GGG | 0.531640 | 3.4:+89132576 | MS.gene054614:CDS |
| AAAGGCAAGAACTCTGATAC+AGG | 0.532612 | 3.4:-89133539 | None:intergenic |
| CTGGACAGTCTCTAACGCTC+TGG | 0.532621 | 3.4:-89132636 | None:intergenic |
| TGACAACATGTAAGCCTCAT+GGG | 0.533383 | 3.4:-89138917 | None:intergenic |
| GCTAAATCCTTTATAAACAA+AGG | 0.533484 | 3.4:-89132664 | None:intergenic |
| TATGAAGACATTAATGGCAA+TGG | 0.534606 | 3.4:+89133087 | MS.gene054614:CDS |
| GAGGAATGTTGCGTTGAAGG+AGG | 0.539110 | 3.4:+89131073 | MS.gene054614:CDS |
| CTGCTAAGGAACTTGGGGCA+TGG | 0.539790 | 3.4:-89132729 | None:intergenic |
| AGTTGGATAAGGGTGAAACA+TGG | 0.540744 | 3.4:+89134667 | MS.gene054614:CDS |
| AGTTGCGAAGGGGAATTCGT+TGG | 0.541353 | 3.4:-89130646 | None:intergenic |
| ATAAATTGTGGACGACAACT+CGG | 0.545434 | 3.4:-89132524 | None:intergenic |
| GCAACAAACGCATTCAAAGT+AGG | 0.550007 | 3.4:-89130924 | None:intergenic |
| GTGACCAAATCAGCCAAATG+AGG | 0.550506 | 3.4:-89139669 | None:intergenic |
| GAAAAGCATCCACAAAATGA+AGG | 0.551030 | 3.4:-89137118 | None:intergenic |
| ATTCCATGAGAATAAGGAAG+AGG | 0.551317 | 3.4:-89130739 | None:intergenic |
| GTGTTTGCAATGAGTTCAAG+AGG | 0.553383 | 3.4:-89136640 | None:intergenic |
| ATCCAAATTCATTCACTCAC+AGG | 0.558285 | 3.4:+89133365 | MS.gene054614:CDS |
| TTGACAACATGTAAGCCTCA+TGG | 0.559907 | 3.4:-89138918 | None:intergenic |
| AATCATCCTTTGCAGGGTAG+GGG | 0.560554 | 3.4:+89137047 | MS.gene054614:intron |
| GTTGGATAAGGGTGAAACAT+GGG | 0.561179 | 3.4:+89134668 | MS.gene054614:CDS |
| CAAGATTGGGGAAAGCCTCT+GGG | 0.561372 | 3.4:+89139769 | MS.gene054614:CDS |
| AGTAGAAATACCTGAACTGC+AGG | 0.561999 | 3.4:-89133435 | None:intergenic |
| TGTTAAGAAAGTTGAAACTA+CGG | 0.562879 | 3.4:+89130887 | MS.gene054614:CDS |
| TAAATTTAAATAAGATACAA+AGG | 0.564848 | 3.4:-89132947 | None:intergenic |
| AAGAACGACTCCACTACCTC+TGG | 0.566170 | 3.4:+89133619 | MS.gene054614:CDS |
| CTCGTGTATATTAGGTGATG+CGG | 0.569304 | 3.4:+89136823 | MS.gene054614:intron |
| GGCAATTAAGAAGTACAACC+AGG | 0.569527 | 3.4:+89136666 | MS.gene054614:CDS |
| TTCAAAGTAGGATTAGGTCT+CGG | 0.569909 | 3.4:-89130912 | None:intergenic |
| AGAAGAAGGAAATGGGCATG+AGG | 0.573659 | 3.4:+89132575 | MS.gene054614:CDS |
| TGAACAGCCAAGTCTGCTGC+AGG | 0.575021 | 3.4:-89131651 | None:intergenic |
| AGAGTGCTGTTTCTCTGGAA+AGG | 0.575452 | 3.4:-89133557 | None:intergenic |
| TCCTTAGCAGCATGTAGCAG+AGG | 0.576081 | 3.4:+89132742 | MS.gene054614:CDS |
| GCAATGAGTTCAAGAGGCCA+AGG | 0.576107 | 3.4:-89136634 | None:intergenic |
| CTAGCTGGACTTATTGGTCA+TGG | 0.577169 | 3.4:+89132865 | MS.gene054614:CDS |
| ATCTTATTTAAATTTAAATG+CGG | 0.577484 | 3.4:+89132954 | MS.gene054614:CDS |
| ATCTTGAAGCAACACTTCCC+GGG | 0.581560 | 3.4:-89139752 | None:intergenic |
| CTGAACTGCAGGCAAAATGG+TGG | 0.583006 | 3.4:-89133424 | None:intergenic |
| GGAATCACCGACATAAATTG+TGG | 0.583520 | 3.4:-89132536 | None:intergenic |
| AACACTTCCCGGGCCAGATG+TGG | 0.583916 | 3.4:-89139742 | None:intergenic |
| AGTGGCCAAATCTCTGGATG+AGG | 0.588149 | 3.4:+89138885 | MS.gene054614:CDS |
| ATCCAGTGAAGAACTTCTGA+AGG | 0.591637 | 3.4:-89133581 | None:intergenic |
| CTCAAAATATACATCAGGGA+TGG | 0.591850 | 3.4:-89131613 | None:intergenic |
| GATCATCTTACCTCATCAGA+AGG | 0.594156 | 3.4:-89131924 | None:intergenic |
| TTGCCTTCTTTAAATGACGA+GGG | 0.597136 | 3.4:+89133255 | MS.gene054614:CDS |
| GGCAGAAGTATTTACAAACA+TGG | 0.599148 | 3.4:-89132891 | None:intergenic |
| TCTGTCTCCTTCTCATAGAG+TGG | 0.599169 | 3.4:+89132807 | MS.gene054614:CDS |
| AAGGAAGAGGAGTTGCGAAG+GGG | 0.599332 | 3.4:-89130656 | None:intergenic |
| ATGGCAATGGTGATTCAAGA+GGG | 0.600314 | 3.4:+89133100 | MS.gene054614:CDS |
| ATCCAACTTATCGAAAATGA+CGG | 0.605211 | 3.4:-89134652 | None:intergenic |
| AGCCCAACCACTCTATGAGA+AGG | 0.608483 | 3.4:-89132814 | None:intergenic |
| CAAATCTTCTCTGAGTAAGG+TGG | 0.610555 | 3.4:-89133006 | None:intergenic |
| TGCAGGTATACCACAGTGCA+TGG | 0.611318 | 3.4:+89138839 | MS.gene054614:intron |
| TGACCAAATCAGCCAAATGA+GGG | 0.611484 | 3.4:-89139668 | None:intergenic |
| AATCAAGGCAAGGTGTGAGA+TGG | 0.611771 | 3.4:+89139190 | MS.gene054614:CDS |
| TTAATGACCACATCTGGCCC+GGG | 0.612240 | 3.4:+89139735 | MS.gene054614:CDS |
| AATTGTACTAGACAAAGAGA+TGG | 0.613500 | 3.4:-89132602 | None:intergenic |
| ACATGCTGCTAAGGAACTTG+GGG | 0.616224 | 3.4:-89132734 | None:intergenic |
| AATGGCAATGGTGATTCAAG+AGG | 0.620186 | 3.4:+89133099 | MS.gene054614:CDS |
| TCACCAATATGTGATGGACC+GGG | 0.621593 | 3.4:+89137139 | MS.gene054614:CDS |
| CTGTTTCTGCAATCAAGGCA+AGG | 0.622785 | 3.4:+89139180 | MS.gene054614:CDS |
| TGAACTGCAGGCAAAATGGT+GGG | 0.624708 | 3.4:-89133423 | None:intergenic |
| TAAAGTTGATGAATGAATGG+AGG | 0.625985 | 3.4:+89133790 | MS.gene054614:CDS |
| ACTTGATGATCCTTCTGATG+AGG | 0.627081 | 3.4:+89131914 | MS.gene054614:CDS |
| ACAGGAGGAAGCTTATCTCA+TGG | 0.628318 | 3.4:+89131821 | MS.gene054614:intron |
| TTGTCGTCCACAATTTATGT+CGG | 0.632408 | 3.4:+89132529 | MS.gene054614:CDS |
| CAAACTCAAAATATACATCA+GGG | 0.637105 | 3.4:-89131617 | None:intergenic |
| CTTCTCTGAGTAAGGTGGAG+CGG | 0.637116 | 3.4:-89133001 | None:intergenic |
| GAAGAAATGTGTCTTGTACA+AGG | 0.642055 | 3.4:+89131702 | MS.gene054614:CDS |
| TAAAGAAGTCTCAGTTACTG+AGG | 0.643493 | 3.4:+89132444 | MS.gene054614:CDS |
| CCTATTGCGAATAGTTCATG+AGG | 0.646235 | 3.4:+89131590 | MS.gene054614:CDS |
| GTGTCTTGTACAAGGTGGCG+AGG | 0.649705 | 3.4:+89131710 | MS.gene054614:CDS |
| CTTACATGTTGTCAATTCAG+CGG | 0.660863 | 3.4:+89138926 | MS.gene054614:CDS |
| AATTTGTATTAGATAAAGTG+CGG | 0.667497 | 3.4:+89136871 | MS.gene054614:CDS |
| GAAGTGTTGCTTCAAGATTG+GGG | 0.671751 | 3.4:+89139757 | MS.gene054614:CDS |
| CTCAACGATTTGAAGACACG+TGG | 0.672206 | 3.4:-89130859 | None:intergenic |
| GTGTCTATGAATAATGCAGA+TGG | 0.673767 | 3.4:+89131098 | MS.gene054614:CDS |
| AGAAGTCTAAAGCTAAACCA+AGG | 0.673970 | 3.4:-89139124 | None:intergenic |
| TGAGGTCATTGATGCCCATG+AGG | 0.679084 | 3.4:+89138903 | MS.gene054614:CDS |
| AAATATTGTTGAGAGTGATG+GGG | 0.690253 | 3.4:+89136402 | MS.gene054614:CDS |
| TGGCAATGGTGATTCAAGAG+GGG | 0.700226 | 3.4:+89133101 | MS.gene054614:CDS |
| AAAGTCGTGTCAATAACTTG+GGG | 0.709042 | 3.4:+89136461 | MS.gene054614:CDS |
| CAGTACTGCATAATAACCAG+AGG | 0.710633 | 3.4:-89133635 | None:intergenic |
| AACCAAGGATGATATTAACG+CGG | 0.726058 | 3.4:-89139109 | None:intergenic |
| AGTGCGGAGATGGATGTGGA+AGG | 0.735084 | 3.4:+89136887 | MS.gene054614:CDS |
| TGAACTCATTGCAAACACAG+AGG | 0.740791 | 3.4:+89136645 | MS.gene054614:CDS |
| ACTATGTGAAAGTATGACAG+TGG | 0.781798 | 3.4:+89138867 | MS.gene054614:CDS |
| GAAATGTGTCTTGTACAAGG+TGG | 0.783507 | 3.4:+89131705 | MS.gene054614:CDS |
| ATAGTTCACGCCATGCACTG+TGG | 0.784087 | 3.4:-89138849 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 89130588 | 89139794 | 89130588 | ID=MS.gene054614 |
| chr3.4 | mRNA | 89130588 | 89139794 | 89130588 | ID=MS.gene054614.t1;Parent=MS.gene054614 |
| chr3.4 | exon | 89130588 | 89130968 | 89130588 | ID=MS.gene054614.t1.exon1;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89130588 | 89130968 | 89130588 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89131068 | 89131165 | 89131068 | ID=MS.gene054614.t1.exon2;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89131068 | 89131165 | 89131068 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89131569 | 89131731 | 89131569 | ID=MS.gene054614.t1.exon3;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89131569 | 89131731 | 89131569 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89131825 | 89131935 | 89131825 | ID=MS.gene054614.t1.exon4;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89131825 | 89131935 | 89131825 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89132427 | 89133446 | 89132427 | ID=MS.gene054614.t1.exon5;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89132427 | 89133446 | 89132427 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89133530 | 89133682 | 89133530 | ID=MS.gene054614.t1.exon6;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89133530 | 89133682 | 89133530 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89133762 | 89133861 | 89133762 | ID=MS.gene054614.t1.exon7;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89133762 | 89133861 | 89133762 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89134628 | 89134722 | 89134628 | ID=MS.gene054614.t1.exon8;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89134628 | 89134722 | 89134628 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89136331 | 89136519 | 89136331 | ID=MS.gene054614.t1.exon9;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89136331 | 89136519 | 89136331 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89136631 | 89136687 | 89136631 | ID=MS.gene054614.t1.exon10;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89136631 | 89136687 | 89136631 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89136837 | 89136908 | 89136837 | ID=MS.gene054614.t1.exon11;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89136837 | 89136908 | 89136837 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89137062 | 89137160 | 89137062 | ID=MS.gene054614.t1.exon12;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89137062 | 89137160 | 89137062 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89138844 | 89138975 | 89138844 | ID=MS.gene054614.t1.exon13;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89138844 | 89138975 | 89138844 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89139092 | 89139265 | 89139092 | ID=MS.gene054614.t1.exon14;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89139092 | 89139265 | 89139092 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| chr3.4 | exon | 89139636 | 89139794 | 89139636 | ID=MS.gene054614.t1.exon15;Parent=MS.gene054614.t1 |
| chr3.4 | CDS | 89139636 | 89139794 | 89139636 | ID=cds.MS.gene054614.t1;Parent=MS.gene054614.t1 |
| Gene Sequence |
| Protein sequence |
