Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42333.t1 | XP_013462130.1 | 94.9 | 138 | 7 | 0 | 1 | 138 | 1 | 138 | 2.20E-69 | 271.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42333.t1 | I3SPV4 | 94.9 | 138 | 7 | 0 | 1 | 138 | 1 | 138 | 1.6e-69 | 271.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059046 | MS.gene42333 | 0.810663 | 1.03E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42333.t1 | MTR_3g111970 | 94.928 | 138 | 7 | 0 | 1 | 138 | 1 | 138 | 6.04e-96 | 273 |
| MS.gene42333.t1 | MTR_3g111970 | 93.913 | 115 | 7 | 0 | 1 | 115 | 1 | 115 | 3.99e-77 | 224 |
| MS.gene42333.t1 | MTR_1g022395 | 59.722 | 144 | 49 | 4 | 1 | 137 | 1 | 142 | 2.85e-52 | 162 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42333.t1 | AT1G19530 | 45.669 | 127 | 53 | 6 | 1 | 126 | 1 | 112 | 3.51e-20 | 80.5 |
| MS.gene42333.t1 | AT1G19530 | 45.669 | 127 | 53 | 6 | 1 | 126 | 1 | 112 | 3.51e-20 | 80.5 |
Find 43 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAATTAAGTACCTGATTT+AGG | 0.181404 | 3.2:-89346805 | None:intergenic |
| CTGAATAGTCCTTGATAAAT+TGG | 0.192807 | 3.2:-89346999 | None:intergenic |
| GAAGGCATCAATTTCTTCTT+TGG | 0.243740 | 3.2:-89346930 | None:intergenic |
| CAATTTATCAAGGACTATTC+AGG | 0.271260 | 3.2:+89347000 | MS.gene42333:CDS |
| CACGGTATCGTCGTATTTGC+CGG | 0.314261 | 3.2:-89347157 | None:intergenic |
| AAGAAGAAATTGATGCCTTC+TGG | 0.324414 | 3.2:+89346933 | MS.gene42333:CDS |
| GCAGGTGGACCAAGAGCAAC+TGG | 0.363566 | 3.2:+89347428 | MS.gene42333:intron |
| CGCCCCATGAAGAAATCATT+TGG | 0.393233 | 3.2:+89347189 | MS.gene42333:CDS |
| TTGGTAGGAGCTTCCATCAC+TGG | 0.398603 | 3.2:-89347469 | None:intergenic |
| CATGCCAAATGATTTCTTCA+TGG | 0.402591 | 3.2:-89347193 | None:intergenic |
| TCAATCAATGGGTTCTCTAA+TGG | 0.403373 | 3.2:+89346756 | None:intergenic |
| TTCTTCATGGGGCGGGCCAA+GGG | 0.427383 | 3.2:-89347180 | None:intergenic |
| CAGAATCACTTGAAAGGAAT+CGG | 0.429094 | 3.2:+89346900 | MS.gene42333:intron |
| ATGCCAAATGATTTCTTCAT+GGG | 0.446330 | 3.2:-89347192 | None:intergenic |
| TTTCTTCATGGGGCGGGCCA+AGG | 0.451036 | 3.2:-89347181 | None:intergenic |
| GCAGTATCACGTAGCCAACT+TGG | 0.457996 | 3.2:+89347504 | MS.gene42333:CDS |
| CAGAAACGCCCAGTTGCTCT+TGG | 0.458612 | 3.2:-89347437 | None:intergenic |
| CACACAACTGATCCTAAATC+AGG | 0.475780 | 3.2:+89346793 | MS.gene42333:CDS |
| AAGTTATTTCCAATTTATCA+AGG | 0.479978 | 3.2:+89346990 | MS.gene42333:CDS |
| CATGGGGCGGGCCAAGGGCA+CGG | 0.483921 | 3.2:-89347175 | None:intergenic |
| TTAGATTTAGATAGACCATT+CGG | 0.484528 | 3.2:-89347569 | None:intergenic |
| AAATGATTTCTTCATGGGGC+GGG | 0.491715 | 3.2:-89347187 | None:intergenic |
| ACGGTATCGTCGTATTTGCC+GGG | 0.502188 | 3.2:-89347156 | None:intergenic |
| ATACGACGATACCGTGCCCT+TGG | 0.513413 | 3.2:+89347164 | MS.gene42333:CDS |
| GGGTTCTCTAATGGCAGGAT+GGG | 0.519019 | 3.2:+89346765 | MS.gene42333:CDS |
| CTCCTTCTTTGATCTCCAGA+AGG | 0.529148 | 3.2:-89346948 | None:intergenic |
| TGCCTTCTGGAGATCAAAGA+AGG | 0.552384 | 3.2:+89346946 | MS.gene42333:CDS |
| TGGGTTCTCTAATGGCAGGA+TGG | 0.562729 | 3.2:+89346764 | MS.gene42333:CDS |
| ATATTTCAGAATCACTTGAA+AGG | 0.563841 | 3.2:+89346894 | MS.gene42333:intron |
| TCAATGGGTTCTCTAATGGC+AGG | 0.564446 | 3.2:+89346760 | None:intergenic |
| CATGAAGAAATCATTTGGCA+TGG | 0.570197 | 3.2:+89347194 | MS.gene42333:CDS |
| CAAATGATTTCTTCATGGGG+CGG | 0.572003 | 3.2:-89347188 | None:intergenic |
| CAGTATCACGTAGCCAACTT+GGG | 0.576698 | 3.2:+89347505 | MS.gene42333:CDS |
| ATACTGCGATGCATACTTGT+TGG | 0.576932 | 3.2:-89347488 | None:intergenic |
| TCTGAATGAACCTCCAGTGA+TGG | 0.578917 | 3.2:+89347456 | MS.gene42333:CDS |
| GTAGGAGCTTCCATCACTGG+AGG | 0.595410 | 3.2:-89347466 | None:intergenic |
| GAATTAGTGCTTGTTCCGAA+TGG | 0.599911 | 3.2:+89347554 | MS.gene42333:CDS |
| TGCCAAATGATTTCTTCATG+GGG | 0.625447 | 3.2:-89347191 | None:intergenic |
| TCAAAATTAAACACAGGACA+TGG | 0.627591 | 3.2:+89347532 | MS.gene42333:CDS |
| TGCGATGCATACTTGTTGGT+AGG | 0.629639 | 3.2:-89347484 | None:intergenic |
| GAGATCAAAGAAGGAGACAG+AGG | 0.641594 | 3.2:+89346955 | MS.gene42333:CDS |
| GGATCATCAAAATTAAACAC+AGG | 0.655378 | 3.2:+89347526 | MS.gene42333:CDS |
| CAGGTGGACCAAGAGCAACT+GGG | 0.675150 | 3.2:+89347429 | MS.gene42333:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCATTTCGATATTTAAT+TGG | + | chr3.2:89347129-89347148 | MS.gene42333:intron | 15.0% |
| !! | CTTAAAAATATGAAGAAAAA+TGG | + | chr3.2:89347240-89347259 | MS.gene42333:CDS | 15.0% |
| !! | CTTTATATTGAATTTGAATA+TGG | + | chr3.2:89347390-89347409 | MS.gene42333:intron | 15.0% |
| !! | ATTAATTAAGTACCTGATTT+AGG | - | chr3.2:89346808-89346827 | None:intergenic | 20.0% |
| !!! | AAGTTATTTCCAATTTATCA+AGG | + | chr3.2:89346990-89347009 | MS.gene42333:CDS | 20.0% |
| !!! | TTAAAAGTATTCATGACAAT+TGG | - | chr3.2:89347356-89347375 | None:intergenic | 20.0% |
| ! | AAAATATGAAGAAAAATGGC+TGG | + | chr3.2:89347244-89347263 | MS.gene42333:CDS | 25.0% |
| ! | AATTTGAATATGGTTGCATT+GGG | + | chr3.2:89347400-89347419 | MS.gene42333:intron | 25.0% |
| ! | ATATTTCAGAATCACTTGAA+AGG | + | chr3.2:89346894-89346913 | MS.gene42333:intron | 25.0% |
| ATGCCAAATGATTTCTTCAT+GGG | - | chr3.2:89347195-89347214 | None:intergenic | 30.0% | |
| CAATTTATCAAGGACTATTC+AGG | + | chr3.2:89347000-89347019 | MS.gene42333:CDS | 30.0% | |
| CATGGATGTTGACAAAAATA+TGG | + | chr3.2:89347212-89347231 | MS.gene42333:CDS | 30.0% | |
| CTGAATAGTCCTTGATAAAT+TGG | - | chr3.2:89347002-89347021 | None:intergenic | 30.0% | |
| GAATTTGAATATGGTTGCAT+TGG | + | chr3.2:89347399-89347418 | MS.gene42333:intron | 30.0% | |
| GGATCATCAAAATTAAACAC+AGG | + | chr3.2:89347526-89347545 | MS.gene42333:CDS | 30.0% | |
| TCAAAATTAAACACAGGACA+TGG | + | chr3.2:89347532-89347551 | MS.gene42333:CDS | 30.0% | |
| ! | GTTTTCTAACGACTAACAAT+TGG | - | chr3.2:89347285-89347304 | None:intergenic | 30.0% |
| !! | TAATTTTGATGATCCCAAGT+TGG | - | chr3.2:89347521-89347540 | None:intergenic | 30.0% |
| AAGAAGAAATTGATGCCTTC+TGG | + | chr3.2:89346933-89346952 | MS.gene42333:CDS | 35.0% | |
| AAGTCTGCAAGTAGTTGAAT+TGG | - | chr3.2:89347330-89347349 | None:intergenic | 35.0% | |
| ATTCAACTACTTGCAGACTT+AGG | + | chr3.2:89347330-89347349 | MS.gene42333:intron | 35.0% | |
| CAGAATCACTTGAAAGGAAT+CGG | + | chr3.2:89346900-89346919 | MS.gene42333:intron | 35.0% | |
| CATGAAGAAATCATTTGGCA+TGG | + | chr3.2:89347194-89347213 | MS.gene42333:CDS | 35.0% | |
| CATGCCAAATGATTTCTTCA+TGG | - | chr3.2:89347196-89347215 | None:intergenic | 35.0% | |
| TGCCAAATGATTTCTTCATG+GGG | - | chr3.2:89347194-89347213 | None:intergenic | 35.0% | |
| TTGTCTTTATGCTCTGTGTT+TGG | + | chr3.2:89346851-89346870 | MS.gene42333:intron | 35.0% | |
| ! | ATGGCTGGTAAATTTTCTCA+TGG | + | chr3.2:89347259-89347278 | MS.gene42333:intron | 35.0% |
| !! | GAAGGCATCAATTTCTTCTT+TGG | - | chr3.2:89346933-89346952 | None:intergenic | 35.0% |
| AAATGATTTCTTCATGGGGC+GGG | - | chr3.2:89347190-89347209 | None:intergenic | 40.0% | |
| ATACTGCGATGCATACTTGT+TGG | - | chr3.2:89347491-89347510 | None:intergenic | 40.0% | |
| CAAATGATTTCTTCATGGGG+CGG | - | chr3.2:89347191-89347210 | None:intergenic | 40.0% | |
| CACACAACTGATCCTAAATC+AGG | + | chr3.2:89346793-89346812 | MS.gene42333:CDS | 40.0% | |
| CGATATTTAATTGGACAGCC+CGG | + | chr3.2:89347138-89347157 | MS.gene42333:intron | 40.0% | |
| GAATTAGTGCTTGTTCCGAA+TGG | + | chr3.2:89347554-89347573 | MS.gene42333:CDS | 40.0% | |
| CAGTATCACGTAGCCAACTT+GGG | + | chr3.2:89347505-89347524 | MS.gene42333:CDS | 45.0% | |
| CGCCCCATGAAGAAATCATT+TGG | + | chr3.2:89347189-89347208 | MS.gene42333:CDS | 45.0% | |
| CTCCTTCTTTGATCTCCAGA+AGG | - | chr3.2:89346951-89346970 | None:intergenic | 45.0% | |
| GAGATCAAAGAAGGAGACAG+AGG | + | chr3.2:89346955-89346974 | MS.gene42333:CDS | 45.0% | |
| TCTGAATGAACCTCCAGTGA+TGG | + | chr3.2:89347456-89347475 | MS.gene42333:CDS | 45.0% | |
| ! | ATGCTCTGTGTTTGGCTTCA+CGG | + | chr3.2:89346859-89346878 | MS.gene42333:intron | 45.0% |
| ! | TGCCTTCTGGAGATCAAAGA+AGG | + | chr3.2:89346946-89346965 | MS.gene42333:CDS | 45.0% |
| ! | TGCGATGCATACTTGTTGGT+AGG | - | chr3.2:89347487-89347506 | None:intergenic | 45.0% |
| ACGGTATCGTCGTATTTGCC+GGG | - | chr3.2:89347159-89347178 | None:intergenic | 50.0% | |
| CACGGTATCGTCGTATTTGC+CGG | - | chr3.2:89347160-89347179 | None:intergenic | 50.0% | |
| GCAGTATCACGTAGCCAACT+TGG | + | chr3.2:89347504-89347523 | MS.gene42333:CDS | 50.0% | |
| GGGTTCTCTAATGGCAGGAT+GGG | + | chr3.2:89346765-89346784 | MS.gene42333:CDS | 50.0% | |
| TGGGTTCTCTAATGGCAGGA+TGG | + | chr3.2:89346764-89346783 | MS.gene42333:CDS | 50.0% | |
| TTGGTAGGAGCTTCCATCAC+TGG | - | chr3.2:89347472-89347491 | None:intergenic | 50.0% | |
| ATACGACGATACCGTGCCCT+TGG | + | chr3.2:89347164-89347183 | MS.gene42333:CDS | 55.0% | |
| CAGAAACGCCCAGTTGCTCT+TGG | - | chr3.2:89347440-89347459 | None:intergenic | 55.0% | |
| CAGGTGGACCAAGAGCAACT+GGG | + | chr3.2:89347429-89347448 | MS.gene42333:intron | 55.0% | |
| GTAGGAGCTTCCATCACTGG+AGG | - | chr3.2:89347469-89347488 | None:intergenic | 55.0% | |
| TGGTTGCATTGGGTGAGTGC+AGG | + | chr3.2:89347410-89347429 | MS.gene42333:intron | 55.0% | |
| TTGCATTGGGTGAGTGCAGG+TGG | + | chr3.2:89347413-89347432 | MS.gene42333:intron | 55.0% | |
| GCAGGTGGACCAAGAGCAAC+TGG | + | chr3.2:89347428-89347447 | MS.gene42333:intron | 60.0% | |
| TTCTTCATGGGGCGGGCCAA+GGG | - | chr3.2:89347183-89347202 | None:intergenic | 60.0% | |
| TTTCTTCATGGGGCGGGCCA+AGG | - | chr3.2:89347184-89347203 | None:intergenic | 60.0% | |
| CATGGGGCGGGCCAAGGGCA+CGG | - | chr3.2:89347178-89347197 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 89346763 | 89347585 | 89346763 | ID=MS.gene42333 |
| chr3.2 | mRNA | 89346763 | 89347585 | 89346763 | ID=MS.gene42333.t1;Parent=MS.gene42333 |
| chr3.2 | exon | 89346763 | 89346814 | 89346763 | ID=MS.gene42333.t1.exon1;Parent=MS.gene42333.t1 |
| chr3.2 | CDS | 89346763 | 89346814 | 89346763 | ID=cds.MS.gene42333.t1;Parent=MS.gene42333.t1 |
| chr3.2 | exon | 89346903 | 89347021 | 89346903 | ID=MS.gene42333.t1.exon2;Parent=MS.gene42333.t1 |
| chr3.2 | CDS | 89346903 | 89347021 | 89346903 | ID=cds.MS.gene42333.t1;Parent=MS.gene42333.t1 |
| chr3.2 | exon | 89347156 | 89347265 | 89347156 | ID=MS.gene42333.t1.exon3;Parent=MS.gene42333.t1 |
| chr3.2 | CDS | 89347156 | 89347265 | 89347156 | ID=cds.MS.gene42333.t1;Parent=MS.gene42333.t1 |
| chr3.2 | exon | 89347432 | 89347585 | 89347432 | ID=MS.gene42333.t1.exon4;Parent=MS.gene42333.t1 |
| chr3.2 | CDS | 89347432 | 89347585 | 89347432 | ID=cds.MS.gene42333.t1;Parent=MS.gene42333.t1 |
| Gene Sequence |
| Protein sequence |
