Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059046.t1 | ADB44209.1 | 100 | 157 | 0 | 0 | 1 | 157 | 1 | 157 | 5.10E-80 | 307 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059046.t1 | Q06931 | 87.3 | 157 | 20 | 0 | 1 | 157 | 1 | 157 | 4.4e-72 | 271.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059046.t1 | D2Y175 | 100.0 | 157 | 0 | 0 | 1 | 157 | 1 | 157 | 3.7e-80 | 307.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056647 | MS.gene059046 | 0.822857 | 1.89E-53 | -1.69E-46 |
| MS.gene058170 | MS.gene059046 | 0.891299 | 4.51E-74 | -1.69E-46 |
| MS.gene058172 | MS.gene059046 | 0.91981 | 2.82E-87 | -1.69E-46 |
| MS.gene058174 | MS.gene059046 | 0.890746 | 7.46E-74 | -1.69E-46 |
| MS.gene059046 | MS.gene071844 | 0.90173 | 1.99E-78 | -1.69E-46 |
| MS.gene059046 | MS.gene25041 | 0.818331 | 2.07E-52 | -1.69E-46 |
| MS.gene059046 | MS.gene33522 | 0.821803 | 3.32E-53 | -1.69E-46 |
| MS.gene059046 | MS.gene42333 | 0.810663 | 1.03E-50 | -1.69E-46 |
| MS.gene059046 | MS.gene45737 | 0.8143 | 1.65E-51 | -1.69E-46 |
| MS.gene059046 | MS.gene81957 | 0.805439 | 1.33E-49 | -1.69E-46 |
| MS.gene059046 | MS.gene97180 | 0.806933 | 6.47E-50 | -1.69E-46 |
| MS.gene059046 | MS.gene97562 | 0.804342 | 2.26E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059046.t1 | MTR_2g035190 | 96.815 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 3.19e-107 | 302 |
| MS.gene059046.t1 | MTR_2g035320 | 95.541 | 157 | 7 | 0 | 1 | 157 | 1 | 157 | 8.01e-107 | 301 |
| MS.gene059046.t1 | MTR_2g035210 | 91.083 | 157 | 14 | 0 | 1 | 157 | 1 | 157 | 2.95e-101 | 287 |
| MS.gene059046.t1 | MTR_2g035220 | 89.809 | 157 | 16 | 0 | 1 | 157 | 1 | 157 | 1.36e-98 | 281 |
| MS.gene059046.t1 | MTR_6g033450 | 66.242 | 157 | 53 | 0 | 1 | 157 | 1 | 157 | 2.80e-70 | 209 |
| MS.gene059046.t1 | MTR_4g120970 | 66.242 | 157 | 53 | 0 | 1 | 157 | 1 | 157 | 2.80e-70 | 209 |
| MS.gene059046.t1 | MTR_2g035100 | 63.694 | 157 | 57 | 0 | 1 | 157 | 1 | 157 | 9.49e-68 | 202 |
| MS.gene059046.t1 | MTR_2g035105 | 63.057 | 157 | 58 | 0 | 1 | 157 | 1 | 157 | 8.03e-67 | 200 |
| MS.gene059046.t1 | MTR_2g035150 | 63.694 | 157 | 57 | 0 | 1 | 157 | 1 | 157 | 8.48e-67 | 200 |
| MS.gene059046.t1 | MTR_2g035130 | 61.783 | 157 | 60 | 0 | 1 | 157 | 1 | 157 | 1.63e-65 | 197 |
| MS.gene059046.t1 | MTR_2g035120 | 59.236 | 157 | 64 | 0 | 1 | 157 | 1 | 157 | 3.14e-62 | 189 |
| MS.gene059046.t1 | MTR_2g035320 | 90.000 | 100 | 10 | 0 | 58 | 157 | 23 | 122 | 3.00e-61 | 185 |
| MS.gene059046.t1 | MTR_2g035170 | 49.045 | 157 | 80 | 0 | 1 | 157 | 1 | 157 | 9.55e-50 | 157 |
| MS.gene059046.t1 | MTR_4g120950 | 42.857 | 161 | 85 | 5 | 1 | 157 | 1 | 158 | 3.61e-32 | 112 |
| MS.gene059046.t1 | MTR_2g435310 | 32.051 | 156 | 105 | 1 | 1 | 155 | 1 | 156 | 8.27e-30 | 106 |
| MS.gene059046.t1 | MTR_4g120760 | 33.758 | 157 | 103 | 1 | 1 | 156 | 1 | 157 | 1.26e-29 | 106 |
| MS.gene059046.t1 | MTR_4g120760 | 33.758 | 157 | 103 | 1 | 1 | 156 | 1 | 157 | 1.38e-29 | 106 |
| MS.gene059046.t1 | MTR_4g120940 | 65.753 | 73 | 25 | 0 | 85 | 157 | 47 | 119 | 1.04e-24 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTAATTGGCTAGTAATT+AGG | 0.164037 | 2.4:+55993735 | None:intergenic |
| GTTTAATTGGCTAGTAATTA+GGG | 0.278519 | 2.4:+55993736 | None:intergenic |
| GGATAGCTTCTTAATAGTTC+CGG | 0.311830 | 2.4:+55994620 | None:intergenic |
| TACAGCTTAGTGGGAGGAAC+AGG | 0.324066 | 2.4:-55993951 | MS.gene059046:CDS |
| CTTGATAAGTCCAGTTCCTT+TGG | 0.329858 | 2.4:+55993785 | None:intergenic |
| TTCAACAATTTCAACACTTT+GGG | 0.350751 | 2.4:+55994656 | None:intergenic |
| CTTCAACAATTTCAACACTT+TGG | 0.364915 | 2.4:+55994655 | None:intergenic |
| TGGGCAGCAGAAATCACCTT+TGG | 0.387864 | 2.4:+55994675 | None:intergenic |
| AGATAGAGTTGCATCACCTT+TGG | 0.394288 | 2.4:+55993833 | None:intergenic |
| GATAGCTTCTTAATAGTTCC+GGG | 0.397748 | 2.4:+55994621 | None:intergenic |
| GTTGAAATTGTTGAAGGAAA+TGG | 0.402462 | 2.4:-55994648 | MS.gene059046:CDS |
| GAGACTAAGGCCAAAGGAAC+TGG | 0.415366 | 2.4:-55993795 | MS.gene059046:CDS |
| AGAGCCTTGTAGAGTTTAGC+TGG | 0.426244 | 2.4:+55994723 | None:intergenic |
| GGGCAGCAGAAATCACCTTT+GGG | 0.449440 | 2.4:+55994676 | None:intergenic |
| TCAGTGGATGAGGCAAACTT+TGG | 0.464386 | 2.4:-55993981 | MS.gene059046:CDS |
| TGCTAAAACGTAGCCCTCAA+TGG | 0.474394 | 2.4:+55993761 | None:intergenic |
| TGGATATAACTACAGCTTAG+TGG | 0.476164 | 2.4:-55993961 | MS.gene059046:CDS |
| AAAATTGTTGCTGGCTCTGA+TGG | 0.476544 | 2.4:-55993891 | MS.gene059046:CDS |
| CAAAGTGTTGAAATTGTTGA+AGG | 0.502542 | 2.4:-55994654 | MS.gene059046:CDS |
| CAAATTAGATTCAGTGGATG+AGG | 0.505903 | 2.4:-55993991 | MS.gene059046:CDS |
| GGATATAACTACAGCTTAGT+GGG | 0.506709 | 2.4:-55993960 | MS.gene059046:CDS |
| ATCTGAATTACCTTCAACAA+TGG | 0.509250 | 2.4:+55994599 | None:intergenic |
| TAATTTGTGTAGCACAAAGT+TGG | 0.512097 | 2.4:+55994007 | None:intergenic |
| GGACTTATCAAGGCCATTGA+GGG | 0.514208 | 2.4:-55993774 | MS.gene059046:CDS |
| CAAAGGAACTGGACTTATCA+AGG | 0.516806 | 2.4:-55993784 | MS.gene059046:CDS |
| AAGAAGCTATCCATTGTTGA+AGG | 0.523757 | 2.4:-55994609 | MS.gene059046:intron |
| AGCAGTACGTGAGGAGACTA+AGG | 0.536209 | 2.4:-55993808 | MS.gene059046:CDS |
| GAGTTGCATCACCTTTGGTA+TGG | 0.537356 | 2.4:+55993838 | None:intergenic |
| GGCTCCAGCTAAACTCTACA+AGG | 0.556705 | 2.4:-55994727 | MS.gene059046:CDS |
| GCTACACAAATTAGATTCAG+TGG | 0.559555 | 2.4:-55993997 | MS.gene059046:CDS |
| TTTCACTGAAATCTTAACAA+TGG | 0.567421 | 2.4:+55993863 | None:intergenic |
| ATTGTTGCTGGCTCTGATGG+TGG | 0.570543 | 2.4:-55993888 | MS.gene059046:CDS |
| TGGACTTATCAAGGCCATTG+AGG | 0.572485 | 2.4:-55993775 | MS.gene059046:CDS |
| GTTGAAGGAAATGGAGGACC+CGG | 0.599694 | 2.4:-55994639 | MS.gene059046:CDS |
| GTAGAGTTTAGCTGGAGCCA+CGG | 0.602676 | 2.4:+55994731 | None:intergenic |
| TGCTGATGAAATCGTCCCAA+AGG | 0.606260 | 2.4:-55994691 | MS.gene059046:CDS |
| CTTAGTGGGAGGAACAGGGT+TGG | 0.609437 | 2.4:-55993946 | MS.gene059046:CDS |
| GAAATTGTTGAAGGAAATGG+AGG | 0.626795 | 2.4:-55994645 | MS.gene059046:CDS |
| TCAGTGAAATACCATACCAA+AGG | 0.652605 | 2.4:-55993849 | MS.gene059046:CDS |
| CGTGAGGAGACTAAGGCCAA+AGG | 0.670663 | 2.4:-55993801 | MS.gene059046:CDS |
| TCTATCTGAAGCAGTACGTG+AGG | 0.676642 | 2.4:-55993817 | MS.gene059046:CDS |
| ACAGCTTAGTGGGAGGAACA+GGG | 0.680453 | 2.4:-55993950 | MS.gene059046:CDS |
| TATAACTACAGCTTAGTGGG+AGG | 0.697586 | 2.4:-55993957 | MS.gene059046:CDS |
| TGATGAACATGTATCAACCG+TGG | 0.718349 | 2.4:-55994748 | MS.gene059046:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATATAAATAATTATAA+TGG | - | chr2.4:55994464-55994483 | MS.gene059046:intron | 0.0% |
| !!! | AAATAAAAGTATAACATAAA+TGG | + | chr2.4:55994269-55994288 | None:intergenic | 10.0% |
| !!! | TTTGTCAATTTTTAAATAAA+AGG | + | chr2.4:55994060-55994079 | None:intergenic | 10.0% |
| !!! | AATTTAACTTCCTTTTTTTT+TGG | + | chr2.4:55994197-55994216 | None:intergenic | 15.0% |
| !!! | AATTTTATACCCAAAAAAAA+AGG | - | chr2.4:55994184-55994203 | MS.gene059046:intron | 15.0% |
| !!! | ATTTAACTTCCTTTTTTTTT+GGG | + | chr2.4:55994196-55994215 | None:intergenic | 15.0% |
| !! | AGTTAAATTCATTATACCAA+AGG | - | chr2.4:55994146-55994165 | MS.gene059046:intron | 20.0% |
| !!! | TAATTTAACTTTCTTTCCTT+TGG | + | chr2.4:55994165-55994184 | None:intergenic | 20.0% |
| ! | AATAGAATCTTGAGAAATGA+AGG | - | chr2.4:55994292-55994311 | MS.gene059046:intron | 25.0% |
| ! | AGGGTTATGTCTAATAATTA+TGG | - | chr2.4:55994337-55994356 | MS.gene059046:intron | 25.0% |
| ! | ATAATTATTAGACATAACCC+TGG | + | chr2.4:55994338-55994357 | None:intergenic | 25.0% |
| ! | TATAATGGTTATATTGCAGA+TGG | - | chr2.4:55994479-55994498 | MS.gene059046:intron | 25.0% |
| ! | TTCAACAATTTCAACACTTT+GGG | + | chr2.4:55993861-55993880 | None:intergenic | 25.0% |
| ! | TTCAGATGATTAATGATACT+CGG | - | chr2.4:55994376-55994395 | MS.gene059046:intron | 25.0% |
| ! | TTTCACTGAAATCTTAACAA+TGG | + | chr2.4:55994654-55994673 | None:intergenic | 25.0% |
| !!! | CATCTGAATCATTTTTTTGA+AGG | + | chr2.4:55994364-55994383 | None:intergenic | 25.0% |
| ATCTGAATTACCTTCAACAA+TGG | + | chr2.4:55993918-55993937 | None:intergenic | 30.0% | |
| CTTCAACAATTTCAACACTT+TGG | + | chr2.4:55993862-55993881 | None:intergenic | 30.0% | |
| GTTGAAATTGTTGAAGGAAA+TGG | - | chr2.4:55993866-55993885 | MS.gene059046:CDS | 30.0% | |
| ! | TAATTTGTGTAGCACAAAGT+TGG | + | chr2.4:55994510-55994529 | None:intergenic | 30.0% |
| ! | TTTGAGACAAAAATTGTTGC+TGG | - | chr2.4:55994614-55994633 | MS.gene059046:CDS | 30.0% |
| !! | CAAAGTGTTGAAATTGTTGA+AGG | - | chr2.4:55993860-55993879 | MS.gene059046:CDS | 30.0% |
| !! | CTTCATGACTATTCTAGAAT+TGG | + | chr2.4:55994246-55994265 | None:intergenic | 30.0% |
| AAGAAGCTATCCATTGTTGA+AGG | - | chr2.4:55993905-55993924 | MS.gene059046:CDS | 35.0% | |
| ATAATACATAAGCTCGTTCC+AGG | - | chr2.4:55994317-55994336 | MS.gene059046:intron | 35.0% | |
| CAAATTAGATTCAGTGGATG+AGG | - | chr2.4:55994523-55994542 | MS.gene059046:intron | 35.0% | |
| GAAATTGTTGAAGGAAATGG+AGG | - | chr2.4:55993869-55993888 | MS.gene059046:CDS | 35.0% | |
| GATAGCTTCTTAATAGTTCC+GGG | + | chr2.4:55993896-55993915 | None:intergenic | 35.0% | |
| GCTACACAAATTAGATTCAG+TGG | - | chr2.4:55994517-55994536 | MS.gene059046:intron | 35.0% | |
| GGATAGCTTCTTAATAGTTC+CGG | + | chr2.4:55993897-55993916 | None:intergenic | 35.0% | |
| GGATATAACTACAGCTTAGT+GGG | - | chr2.4:55994554-55994573 | MS.gene059046:intron | 35.0% | |
| TAATACATAAGCTCGTTCCA+GGG | - | chr2.4:55994318-55994337 | MS.gene059046:intron | 35.0% | |
| TCAGTGAAATACCATACCAA+AGG | - | chr2.4:55994665-55994684 | MS.gene059046:CDS | 35.0% | |
| TGGATATAACTACAGCTTAG+TGG | - | chr2.4:55994553-55994572 | MS.gene059046:intron | 35.0% | |
| ! | TTGTACCGACAAACTTTGAT+CGG | + | chr2.4:55994019-55994038 | None:intergenic | 35.0% |
| AGATAGAGTTGCATCACCTT+TGG | + | chr2.4:55994684-55994703 | None:intergenic | 40.0% | |
| CAAAGGAACTGGACTTATCA+AGG | - | chr2.4:55994730-55994749 | MS.gene059046:CDS | 40.0% | |
| CTTGATAAGTCCAGTTCCTT+TGG | + | chr2.4:55994732-55994751 | None:intergenic | 40.0% | |
| GACAAACATGATGCGACAAA+TGG | - | chr2.4:55994074-55994093 | MS.gene059046:intron | 40.0% | |
| GACAAACTTTGATCGGTTGT+CGG | + | chr2.4:55994012-55994031 | None:intergenic | 40.0% | |
| GACAACCGATCAAAGTTTGT+CGG | - | chr2.4:55994011-55994030 | MS.gene059046:CDS | 40.0% | |
| TATAACTACAGCTTAGTGGG+AGG | - | chr2.4:55994557-55994576 | MS.gene059046:intron | 40.0% | |
| TGATGAACATGTATCAACCG+TGG | - | chr2.4:55993766-55993785 | MS.gene059046:CDS | 40.0% | |
| !! | AAAATTGTTGCTGGCTCTGA+TGG | - | chr2.4:55994623-55994642 | MS.gene059046:CDS | 40.0% |
| GAGTTGCATCACCTTTGGTA+TGG | + | chr2.4:55994679-55994698 | None:intergenic | 45.0% | |
| GGACTTATCAAGGCCATTGA+GGG | - | chr2.4:55994740-55994759 | MS.gene059046:CDS | 45.0% | |
| TCAGTGGATGAGGCAAACTT+TGG | - | chr2.4:55994533-55994552 | MS.gene059046:intron | 45.0% | |
| TGCTAAAACGTAGCCCTCAA+TGG | + | chr2.4:55994756-55994775 | None:intergenic | 45.0% | |
| TGCTGATGAAATCGTCCCAA+AGG | - | chr2.4:55993823-55993842 | MS.gene059046:CDS | 45.0% | |
| TGGACTTATCAAGGCCATTG+AGG | - | chr2.4:55994739-55994758 | MS.gene059046:CDS | 45.0% | |
| ! | AGAGCCTTGTAGAGTTTAGC+TGG | + | chr2.4:55993794-55993813 | None:intergenic | 45.0% |
| ! | TCTATCTGAAGCAGTACGTG+AGG | - | chr2.4:55994697-55994716 | MS.gene059046:CDS | 45.0% |
| ACAGCTTAGTGGGAGGAACA+GGG | - | chr2.4:55994564-55994583 | MS.gene059046:intron | 50.0% | |
| AGCAGTACGTGAGGAGACTA+AGG | - | chr2.4:55994706-55994725 | MS.gene059046:CDS | 50.0% | |
| GAGACTAAGGCCAAAGGAAC+TGG | - | chr2.4:55994719-55994738 | MS.gene059046:CDS | 50.0% | |
| GGCTCCAGCTAAACTCTACA+AGG | - | chr2.4:55993787-55993806 | MS.gene059046:CDS | 50.0% | |
| GGGCAGCAGAAATCACCTTT+GGG | + | chr2.4:55993841-55993860 | None:intergenic | 50.0% | |
| GTAGAGTTTAGCTGGAGCCA+CGG | + | chr2.4:55993786-55993805 | None:intergenic | 50.0% | |
| GTTGAAGGAAATGGAGGACC+CGG | - | chr2.4:55993875-55993894 | MS.gene059046:CDS | 50.0% | |
| TACAGCTTAGTGGGAGGAAC+AGG | - | chr2.4:55994563-55994582 | MS.gene059046:intron | 50.0% | |
| TGGGCAGCAGAAATCACCTT+TGG | + | chr2.4:55993842-55993861 | None:intergenic | 50.0% | |
| !! | ATTGTTGCTGGCTCTGATGG+TGG | - | chr2.4:55994626-55994645 | MS.gene059046:CDS | 50.0% |
| CGTGAGGAGACTAAGGCCAA+AGG | - | chr2.4:55994713-55994732 | MS.gene059046:CDS | 55.0% | |
| CTTAGTGGGAGGAACAGGGT+TGG | - | chr2.4:55994568-55994587 | MS.gene059046:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 55993746 | 55994790 | 55993746 | ID=MS.gene059046 |
| chr2.4 | mRNA | 55993746 | 55994790 | 55993746 | ID=MS.gene059046.t1;Parent=MS.gene059046 |
| chr2.4 | exon | 55994610 | 55994790 | 55994610 | ID=MS.gene059046.t1.exon1;Parent=MS.gene059046.t1 |
| chr2.4 | CDS | 55994610 | 55994790 | 55994610 | ID=cds.MS.gene059046.t1;Parent=MS.gene059046.t1 |
| chr2.4 | exon | 55993746 | 55994038 | 55993746 | ID=MS.gene059046.t1.exon2;Parent=MS.gene059046.t1 |
| chr2.4 | CDS | 55993746 | 55994038 | 55993746 | ID=cds.MS.gene059046.t1;Parent=MS.gene059046.t1 |
| Gene Sequence |
| Protein sequence |
