Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43020.t1 | XP_024634610.1 | 95.6 | 204 | 9 | 0 | 1 | 204 | 3 | 206 | 1.50E-111 | 412.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43020.t1 | A0A2Z6ND66 | 74.2 | 194 | 45 | 2 | 15 | 203 | 1 | 194 | 4.0e-79 | 303.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050585 | MS.gene43020 | 0.818076 | 2.37E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43020.t1 | MTR_7g033950 | 73.077 | 52 | 14 | 0 | 5 | 56 | 1 | 52 | 7.59e-23 | 89.4 |
| MS.gene43020.t1 | MTR_6g034720 | 97.674 | 43 | 1 | 0 | 1 | 43 | 3 | 45 | 9.37e-23 | 91.7 |
| MS.gene43020.t1 | MTR_2g048845 | 96.552 | 29 | 1 | 0 | 83 | 111 | 91 | 119 | 6.99e-15 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACACTTAAAGCTCAATTTA+AGG | 0.196565 | 8.1:-55840953 | MS.gene43020:CDS |
| AACTTCAACGCCTTCTCGTT+CGG | 0.271228 | 8.1:+55840598 | None:intergenic |
| ACTTCAACGCCTTCTCGTTC+GGG | 0.297833 | 8.1:+55840599 | None:intergenic |
| ATATGGTCGGTGACGCTCTC+GGG | 0.342528 | 8.1:-55840663 | MS.gene43020:CDS |
| GAAGTACTCTGCACAATCTT+CGG | 0.349472 | 8.1:+55840468 | None:intergenic |
| TATTAACTGGGAAGTCAAAT+TGG | 0.365814 | 8.1:-55840498 | MS.gene43020:CDS |
| AATATATTCTTCATCGAAGT+TGG | 0.371227 | 8.1:-55841005 | MS.gene43020:CDS |
| AGATGTCATAAAACATTTAG+AGG | 0.383987 | 8.1:-55840818 | MS.gene43020:CDS |
| CTTTGGTCTGATGGAAGATA+TGG | 0.395508 | 8.1:-55840680 | MS.gene43020:CDS |
| AAGTACTCTGCACAATCTTC+GGG | 0.396672 | 8.1:+55840469 | None:intergenic |
| TCAAGCAGTGGAGCGCATAC+AGG | 0.434092 | 8.1:-55840712 | MS.gene43020:CDS |
| TGAGTTTGATGTTAATATGC+AGG | 0.446844 | 8.1:-55840737 | MS.gene43020:CDS |
| ATGTGTGTGAGGCTATTAAC+TGG | 0.459830 | 8.1:-55840511 | MS.gene43020:CDS |
| TAAACGAACCGTTGTTTGAA+GGG | 0.462537 | 8.1:-55840555 | MS.gene43020:CDS |
| AATGTCAATGATGCAGAAGA+TGG | 0.480844 | 8.1:-55841031 | None:intergenic |
| GATATGGTCGGTGACGCTCT+CGG | 0.482218 | 8.1:-55840664 | MS.gene43020:CDS |
| ATATTAACATCAAACTCATC+CGG | 0.506365 | 8.1:+55840742 | None:intergenic |
| GTAAACGAACCGTTGTTTGA+AGG | 0.506586 | 8.1:-55840556 | MS.gene43020:CDS |
| ATAAAACATTTAGAGGATGT+TGG | 0.508215 | 8.1:-55840811 | MS.gene43020:CDS |
| GTGTGAAAACTTTGGTCTGA+TGG | 0.530989 | 8.1:-55840689 | MS.gene43020:CDS |
| GGAAATAATCCCGAACGAGA+AGG | 0.533851 | 8.1:-55840608 | MS.gene43020:CDS |
| CTTAAAGCTCAATTTAAGGA+AGG | 0.547446 | 8.1:-55840949 | MS.gene43020:CDS |
| ACATCATGGTGAACAAGAGC+CGG | 0.548529 | 8.1:-55840761 | MS.gene43020:CDS |
| CATACAGGGTGTGAAAACTT+TGG | 0.549792 | 8.1:-55840697 | MS.gene43020:CDS |
| CAAGCAGTGGAGCGCATACA+GGG | 0.574660 | 8.1:-55840711 | MS.gene43020:CDS |
| AATATGCAGGCTTCAAGCAG+TGG | 0.588779 | 8.1:-55840724 | MS.gene43020:CDS |
| TATTATGTGTGGAGACATCA+TGG | 0.594508 | 8.1:-55840775 | MS.gene43020:CDS |
| AGTTAATAGCCTCACACACA+TGG | 0.596540 | 8.1:+55840513 | None:intergenic |
| GTGAATCTGTCTTACAATGA+GGG | 0.597878 | 8.1:-55840640 | MS.gene43020:CDS |
| GGTGAATCTGTCTTACAATG+AGG | 0.609825 | 8.1:-55840641 | MS.gene43020:CDS |
| CGAAGTTATCCATGTGTGTG+AGG | 0.614191 | 8.1:-55840522 | MS.gene43020:CDS |
| TATGGTCGGTGACGCTCTCG+GGG | 0.617903 | 8.1:-55840662 | MS.gene43020:CDS |
| TTACAATGAGGGCATTGAAG+AGG | 0.617956 | 8.1:-55840629 | MS.gene43020:CDS |
| TTATGGAGGATTATTATGTG+TGG | 0.622827 | 8.1:-55840786 | MS.gene43020:CDS |
| AGTACTTCACGAAAATGATG+AGG | 0.648038 | 8.1:-55840453 | MS.gene43020:CDS |
| GTACTTCACGAAAATGATGA+GGG | 0.657153 | 8.1:-55840452 | MS.gene43020:CDS |
| CTACACGTACATTTAAGACA+CGG | 0.675744 | 8.1:+55840859 | None:intergenic |
| CGTGTCTTAAATGTACGTGT+AGG | 0.676168 | 8.1:-55840858 | MS.gene43020:CDS |
| TCAGACGACCCTTCAAACAA+CGG | 0.704592 | 8.1:+55840547 | None:intergenic |
| TGTGTGTGAGGCTATTAACT+GGG | 0.726706 | 8.1:-55840510 | MS.gene43020:CDS |
| GGTCTGATGGAAGATATGGT+CGG | 0.737621 | 8.1:-55840676 | MS.gene43020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATATATTCTTCATCGAAGT+TGG | - | chr8.1:55840451-55840470 | MS.gene43020:CDS | 25.0% |
| ! | AGATGTCATAAAACATTTAG+AGG | - | chr8.1:55840638-55840657 | MS.gene43020:CDS | 25.0% |
| ! | ATAAAACATTTAGAGGATGT+TGG | - | chr8.1:55840645-55840664 | MS.gene43020:CDS | 25.0% |
| ! | ATATTAACATCAAACTCATC+CGG | + | chr8.1:55840717-55840736 | None:intergenic | 25.0% |
| ! | TCTAAAAATATCTTGTGACA+TGG | + | chr8.1:55840556-55840575 | None:intergenic | 25.0% |
| !! | AAATGTTTTATGACATCTTC+TGG | + | chr8.1:55840636-55840655 | None:intergenic | 25.0% |
| !! | AATGTTTTATGACATCTTCT+GGG | + | chr8.1:55840635-55840654 | None:intergenic | 25.0% |
| ATGTATGATAGAAAAAGACC+GGG | - | chr8.1:55840474-55840493 | MS.gene43020:CDS | 30.0% | |
| CTTAAAGCTCAATTTAAGGA+AGG | - | chr8.1:55840507-55840526 | MS.gene43020:CDS | 30.0% | |
| GACACTTAAAGCTCAATTTA+AGG | - | chr8.1:55840503-55840522 | MS.gene43020:CDS | 30.0% | |
| GATGTATGATAGAAAAAGAC+CGG | - | chr8.1:55840473-55840492 | MS.gene43020:CDS | 30.0% | |
| TATTAACTGGGAAGTCAAAT+TGG | - | chr8.1:55840958-55840977 | MS.gene43020:CDS | 30.0% | |
| TGAGTTTGATGTTAATATGC+AGG | - | chr8.1:55840719-55840738 | MS.gene43020:CDS | 30.0% | |
| TTATGGAGGATTATTATGTG+TGG | - | chr8.1:55840670-55840689 | MS.gene43020:CDS | 30.0% | |
| !!! | ACAAGATATTTTTAGAAGCG+AGG | - | chr8.1:55840560-55840579 | MS.gene43020:CDS | 30.0% |
| !!! | CAAGATATTTTTAGAAGCGA+GGG | - | chr8.1:55840561-55840580 | MS.gene43020:CDS | 30.0% |
| !!! | TTTAGAGGATGTTGGTTTTA+TGG | - | chr8.1:55840653-55840672 | MS.gene43020:CDS | 30.0% |
| AGTACTTCACGAAAATGATG+AGG | - | chr8.1:55841003-55841022 | MS.gene43020:CDS | 35.0% | |
| GTACTTCACGAAAATGATGA+GGG | - | chr8.1:55841004-55841023 | MS.gene43020:CDS | 35.0% | |
| GTGAATCTGTCTTACAATGA+GGG | - | chr8.1:55840816-55840835 | MS.gene43020:CDS | 35.0% | |
| TAAACGAACCGTTGTTTGAA+GGG | - | chr8.1:55840901-55840920 | MS.gene43020:CDS | 35.0% | |
| TATTATGTGTGGAGACATCA+TGG | - | chr8.1:55840681-55840700 | MS.gene43020:CDS | 35.0% | |
| TGTATGATAGAAAAAGACCG+GGG | - | chr8.1:55840475-55840494 | MS.gene43020:CDS | 35.0% | |
| ! | CTACACGTACATTTAAGACA+CGG | + | chr8.1:55840600-55840619 | None:intergenic | 35.0% |
| AAGTACTCTGCACAATCTTC+GGG | + | chr8.1:55840990-55841009 | None:intergenic | 40.0% | |
| AGTTAATAGCCTCACACACA+TGG | + | chr8.1:55840946-55840965 | None:intergenic | 40.0% | |
| ATAGAAAAAGACCGGGGAAA+AGG | - | chr8.1:55840481-55840500 | MS.gene43020:CDS | 40.0% | |
| ATGTGTGTGAGGCTATTAAC+TGG | - | chr8.1:55840945-55840964 | MS.gene43020:CDS | 40.0% | |
| CATACAGGGTGTGAAAACTT+TGG | - | chr8.1:55840759-55840778 | MS.gene43020:CDS | 40.0% | |
| CGTGTCTTAAATGTACGTGT+AGG | - | chr8.1:55840598-55840617 | MS.gene43020:CDS | 40.0% | |
| CTTTGGTCTGATGGAAGATA+TGG | - | chr8.1:55840776-55840795 | MS.gene43020:CDS | 40.0% | |
| GGTGAATCTGTCTTACAATG+AGG | - | chr8.1:55840815-55840834 | MS.gene43020:CDS | 40.0% | |
| GTAAACGAACCGTTGTTTGA+AGG | - | chr8.1:55840900-55840919 | MS.gene43020:CDS | 40.0% | |
| GTGTGAAAACTTTGGTCTGA+TGG | - | chr8.1:55840767-55840786 | MS.gene43020:CDS | 40.0% | |
| TGTGTGTGAGGCTATTAACT+GGG | - | chr8.1:55840946-55840965 | MS.gene43020:CDS | 40.0% | |
| ! | GAAGTACTCTGCACAATCTT+CGG | + | chr8.1:55840991-55841010 | None:intergenic | 40.0% |
| !! | TTACAATGAGGGCATTGAAG+AGG | - | chr8.1:55840827-55840846 | MS.gene43020:CDS | 40.0% |
| !!! | AGAGGATGTTGGTTTTATGG+AGG | - | chr8.1:55840656-55840675 | MS.gene43020:CDS | 40.0% |
| !!! | GATATTTTTAGAAGCGAGGG+AGG | - | chr8.1:55840564-55840583 | MS.gene43020:CDS | 40.0% |
| AACTTCAACGCCTTCTCGTT+CGG | + | chr8.1:55840861-55840880 | None:intergenic | 45.0% | |
| AATATGCAGGCTTCAAGCAG+TGG | - | chr8.1:55840732-55840751 | MS.gene43020:CDS | 45.0% | |
| ACATCATGGTGAACAAGAGC+CGG | - | chr8.1:55840695-55840714 | MS.gene43020:CDS | 45.0% | |
| CGAAGTTATCCATGTGTGTG+AGG | - | chr8.1:55840934-55840953 | MS.gene43020:CDS | 45.0% | |
| GGAAATAATCCCGAACGAGA+AGG | - | chr8.1:55840848-55840867 | MS.gene43020:CDS | 45.0% | |
| GGTCTGATGGAAGATATGGT+CGG | - | chr8.1:55840780-55840799 | MS.gene43020:CDS | 45.0% | |
| TCAGACGACCCTTCAAACAA+CGG | + | chr8.1:55840912-55840931 | None:intergenic | 45.0% | |
| !! | GCTTTAAGTGTCCTTTTCCC+CGG | + | chr8.1:55840495-55840514 | None:intergenic | 45.0% |
| ACTTCAACGCCTTCTCGTTC+GGG | + | chr8.1:55840860-55840879 | None:intergenic | 50.0% | |
| ATATGGTCGGTGACGCTCTC+GGG | - | chr8.1:55840793-55840812 | MS.gene43020:CDS | 55.0% | |
| CAAGCAGTGGAGCGCATACA+GGG | - | chr8.1:55840745-55840764 | MS.gene43020:CDS | 55.0% | |
| GATATGGTCGGTGACGCTCT+CGG | - | chr8.1:55840792-55840811 | MS.gene43020:CDS | 55.0% | |
| TCAAGCAGTGGAGCGCATAC+AGG | - | chr8.1:55840744-55840763 | MS.gene43020:CDS | 55.0% | |
| TATGGTCGGTGACGCTCTCG+GGG | - | chr8.1:55840794-55840813 | MS.gene43020:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 55840432 | 55841046 | 55840432 | ID=MS.gene43020 |
| chr8.1 | mRNA | 55840432 | 55841046 | 55840432 | ID=MS.gene43020.t1;Parent=MS.gene43020 |
| chr8.1 | exon | 55840432 | 55841046 | 55840432 | ID=MS.gene43020.t1.exon1;Parent=MS.gene43020.t1 |
| chr8.1 | CDS | 55840432 | 55841046 | 55840432 | ID=cds.MS.gene43020.t1;Parent=MS.gene43020.t1 |
| Gene Sequence |
| Protein sequence |
