Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43207.t1 | XP_013447843.1 | 86.7 | 255 | 29 | 1 | 1 | 255 | 1 | 250 | 1.50E-113 | 419.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43207.t1 | A2XID3 | 68.7 | 195 | 59 | 2 | 62 | 255 | 47 | 240 | 1.2e-71 | 271.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43207.t1 | A0A072TWG0 | 86.7 | 255 | 29 | 1 | 1 | 255 | 1 | 250 | 1.1e-113 | 419.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053218 | MS.gene43207 | 0.801237 | 9.90E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43207.t1 | MTR_7g417750 | 91.373 | 255 | 17 | 1 | 1 | 255 | 1 | 250 | 1.23e-172 | 476 |
| MS.gene43207.t1 | MTR_4g069020 | 75.676 | 259 | 57 | 4 | 1 | 255 | 1 | 257 | 4.80e-140 | 394 |
| MS.gene43207.t1 | MTR_6g017155 | 61.176 | 255 | 93 | 3 | 1 | 255 | 1 | 249 | 4.13e-103 | 300 |
| MS.gene43207.t1 | MTR_5g053950 | 73.404 | 188 | 49 | 1 | 68 | 255 | 66 | 252 | 1.21e-102 | 298 |
| MS.gene43207.t1 | MTR_6g017165 | 62.447 | 237 | 83 | 4 | 1 | 237 | 1 | 231 | 6.50e-100 | 291 |
| MS.gene43207.t1 | MTR_8g062760 | 78.814 | 118 | 15 | 1 | 1 | 118 | 1 | 108 | 8.89e-58 | 180 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43207.t1 | AT1G13280 | 64.362 | 188 | 64 | 3 | 70 | 255 | 68 | 254 | 1.26e-84 | 253 |
| MS.gene43207.t1 | AT3G25780 | 60.190 | 211 | 69 | 5 | 47 | 255 | 61 | 258 | 9.45e-83 | 248 |
| MS.gene43207.t1 | AT3G25770 | 58.824 | 204 | 75 | 4 | 58 | 255 | 53 | 253 | 2.16e-81 | 245 |
| MS.gene43207.t1 | AT3G25760 | 49.425 | 261 | 119 | 6 | 1 | 255 | 1 | 254 | 1.34e-79 | 240 |
Find 44 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCCTCATGCTGTCATTGC+TGG | 0.269913 | 7.2:+79367447 | MS.gene43207:CDS |
| TGTCAATTCTCTTGGTGATT+TGG | 0.278830 | 7.2:+79366627 | MS.gene43207:CDS |
| CTAGCTGTGACTGGTGGTTC+TGG | 0.279570 | 7.2:+79367255 | MS.gene43207:CDS |
| TGAATGCATTGTTCATCTTT+AGG | 0.298780 | 7.2:-79366348 | None:intergenic |
| AGTGACCATAATCACCAAAA+TGG | 0.347553 | 7.2:-79367188 | None:intergenic |
| GCTGTCTACAGTTTCCATTT+TGG | 0.352706 | 7.2:+79367174 | MS.gene43207:CDS |
| TCCTCAGGATAGGTTAAATA+TGG | 0.365478 | 7.2:-79367225 | None:intergenic |
| AAACGTATTGGTATAACAAC+TGG | 0.370481 | 7.2:+79367099 | MS.gene43207:CDS |
| AATGTGTAGAAAATCTTAAA+AGG | 0.374409 | 7.2:-79367327 | None:intergenic |
| GATTTGCCTCAAGAGCTTCT+TGG | 0.394018 | 7.2:+79367369 | MS.gene43207:CDS |
| ACAGCAGGAGAAGGCTCAAC+AGG | 0.409306 | 7.2:-79367396 | None:intergenic |
| TGCCAGCATACTCATTTGCT+TGG | 0.429821 | 7.2:-79366420 | None:intergenic |
| TTGCTTAATCTCAAGTAAGC+TGG | 0.430132 | 7.2:-79366598 | None:intergenic |
| TATGGTCACTTAGCTGTTCA+AGG | 0.466394 | 7.2:+79367201 | MS.gene43207:CDS |
| AAGGGTGTGAAAGCAGATGA+GGG | 0.471406 | 7.2:-79366295 | None:intergenic |
| AATAAAAGTGTCAATTCTCT+TGG | 0.471904 | 7.2:+79366619 | MS.gene43207:CDS |
| TGCTTAATCTCAAGTAAGCT+GGG | 0.477724 | 7.2:-79366597 | None:intergenic |
| GCAGGAGAAGCTTCAACAGC+AGG | 0.485103 | 7.2:-79367411 | None:intergenic |
| GAAGGGTGTGAAAGCAGATG+AGG | 0.507693 | 7.2:-79366296 | None:intergenic |
| GTTGGATTAACAAAGGAAGA+TGG | 0.515342 | 7.2:-79366238 | None:intergenic |
| CAACACATTGCAGAAAAGAA+AGG | 0.527550 | 7.2:+79367138 | MS.gene43207:CDS |
| AGATGAGTTTGATGAAGTGA+AGG | 0.532217 | 7.2:-79366314 | None:intergenic |
| GGCTCAAGAGACTTAGCAGC+AGG | 0.536989 | 7.2:-79367429 | None:intergenic |
| TAGATAAGTGTCCTCAGGAT+AGG | 0.537416 | 7.2:-79367235 | None:intergenic |
| AGCCAAGCAAATGAGTATGC+TGG | 0.550294 | 7.2:+79366418 | MS.gene43207:CDS |
| GCAAATGAGTATGCTGGCAC+TGG | 0.554905 | 7.2:+79366424 | MS.gene43207:CDS |
| GGGGTGGTGGCAAAAGCAGT+TGG | 0.556218 | 7.2:-79366256 | None:intergenic |
| ACAGCTAGATAAGTGTCCTC+AGG | 0.560526 | 7.2:-79367240 | None:intergenic |
| GAAGCTTCAACAGCAGGAGA+AGG | 0.572761 | 7.2:-79367405 | None:intergenic |
| GACACTTATCTAGCTGTGAC+TGG | 0.577356 | 7.2:+79367246 | MS.gene43207:CDS |
| TCAAGTAAGCTGGGCTACCA+CGG | 0.602620 | 7.2:-79366588 | None:intergenic |
| TGAATTATGCAGTTGTACAC+TGG | 0.616396 | 7.2:+79367066 | MS.gene43207:intron |
| TCTCAAAGCATAGCTTGAAG+AGG | 0.618632 | 7.2:-79366209 | None:intergenic |
| GATGAGTTTGATGAAGTGAA+GGG | 0.622372 | 7.2:-79366313 | None:intergenic |
| GGTTTACCAAGAAGCTCTTG+AGG | 0.629043 | 7.2:-79367375 | None:intergenic |
| GGGTGTGAAAGCAGATGAGG+GGG | 0.634086 | 7.2:-79366293 | None:intergenic |
| AAACCAGCAATGACAGCATG+AGG | 0.634146 | 7.2:-79367450 | None:intergenic |
| AAAAGCAGTTGGATTAACAA+AGG | 0.635467 | 7.2:-79366245 | None:intergenic |
| ACTTATCTAGCTGTGACTGG+TGG | 0.640066 | 7.2:+79367249 | MS.gene43207:CDS |
| AGGGTGTGAAAGCAGATGAG+GGG | 0.653243 | 7.2:-79366294 | None:intergenic |
| GAGCTTAATGAGAGGGACCG+TGG | 0.672691 | 7.2:+79366571 | MS.gene43207:CDS |
| ACCATATTTAACCTATCCTG+AGG | 0.673220 | 7.2:+79367224 | MS.gene43207:CDS |
| TGTGTATGAGCTTAATGAGA+GGG | 0.682673 | 7.2:+79366564 | MS.gene43207:CDS |
| ATGTGTATGAGCTTAATGAG+AGG | 0.762359 | 7.2:+79366563 | MS.gene43207:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTGATAGTTTTTTTAT+CGG | + | chr7.2:79366716-79366735 | MS.gene43207:intron | 10.0% |
| !!! | CTATTTTAGAAAAAAATTAA+AGG | + | chr7.2:79366771-79366790 | MS.gene43207:intron | 10.0% |
| !!! | ATCCATAAACAATTTTTTAA+TGG | - | chr7.2:79366807-79366826 | None:intergenic | 15.0% |
| !!! | ATTTTATTTAAGAGGTATTA+AGG | + | chr7.2:79367347-79367366 | MS.gene43207:CDS | 15.0% |
| !!! | TAATTGCAATTTTTTTTCTT+TGG | + | chr7.2:79366859-79366878 | MS.gene43207:intron | 15.0% |
| !!! | TTTTGAAAAACATGTATATT+TGG | - | chr7.2:79366467-79366486 | None:intergenic | 15.0% |
| !!! | TTTTTTTCTAAAATAGTTCA+CGG | - | chr7.2:79366767-79366786 | None:intergenic | 15.0% |
| !! | AATGTGTAGAAAATCTTAAA+AGG | - | chr7.2:79367330-79367349 | None:intergenic | 20.0% |
| !! | CTCCATTAAAAAATTGTTTA+TGG | + | chr7.2:79366802-79366821 | MS.gene43207:intron | 20.0% |
| !! | TGATCACAAAAATAAAATAG+AGG | - | chr7.2:79366694-79366713 | None:intergenic | 20.0% |
| !!! | TTCTACACATTTTATTTAAG+AGG | + | chr7.2:79367339-79367358 | MS.gene43207:CDS | 20.0% |
| !! | TTATACCAATACGTTTTTGT+AGG | - | chr7.2:79367095-79367114 | None:intergenic | 25.0% |
| !!! | AATAAAAGTGTCAATTCTCT+TGG | + | chr7.2:79366619-79366638 | MS.gene43207:CDS | 25.0% |
| !!! | TTTTTTACTTTGTTGCTGAA+GGG | - | chr7.2:79366655-79366674 | None:intergenic | 25.0% |
| !!! | TTTTTTTACTTTGTTGCTGA+AGG | - | chr7.2:79366656-79366675 | None:intergenic | 25.0% |
| AAAAGCAGTTGGATTAACAA+AGG | - | chr7.2:79366248-79366267 | None:intergenic | 30.0% | |
| TGAATGCATTGTTCATCTTT+AGG | - | chr7.2:79366351-79366370 | None:intergenic | 30.0% | |
| ! | AAACGTATTGGTATAACAAC+TGG | + | chr7.2:79367099-79367118 | MS.gene43207:CDS | 30.0% |
| ! | TTTTTACTTTGTTGCTGAAG+GGG | - | chr7.2:79366654-79366673 | None:intergenic | 30.0% |
| !! | AGTTTCCATTTTGGTGATTA+TGG | + | chr7.2:79367183-79367202 | MS.gene43207:CDS | 30.0% |
| !!! | GGAATTTTTGAAGGTGTTAA+AGG | + | chr7.2:79367276-79367295 | MS.gene43207:CDS | 30.0% |
| ACCATATTTAACCTATCCTG+AGG | + | chr7.2:79367224-79367243 | MS.gene43207:CDS | 35.0% | |
| AGATGAGTTTGATGAAGTGA+AGG | - | chr7.2:79366317-79366336 | None:intergenic | 35.0% | |
| AGTGACCATAATCACCAAAA+TGG | - | chr7.2:79367191-79367210 | None:intergenic | 35.0% | |
| ATGTGTATGAGCTTAATGAG+AGG | + | chr7.2:79366563-79366582 | MS.gene43207:CDS | 35.0% | |
| CAACACATTGCAGAAAAGAA+AGG | + | chr7.2:79367138-79367157 | MS.gene43207:CDS | 35.0% | |
| CTAAAATAGTTCACGGAAGT+TGG | - | chr7.2:79366760-79366779 | None:intergenic | 35.0% | |
| GATGAGTTTGATGAAGTGAA+GGG | - | chr7.2:79366316-79366335 | None:intergenic | 35.0% | |
| GTTGGATTAACAAAGGAAGA+TGG | - | chr7.2:79366241-79366260 | None:intergenic | 35.0% | |
| TCCTCAGGATAGGTTAAATA+TGG | - | chr7.2:79367228-79367247 | None:intergenic | 35.0% | |
| TGAATTATGCAGTTGTACAC+TGG | + | chr7.2:79367066-79367085 | MS.gene43207:intron | 35.0% | |
| TGCTTAATCTCAAGTAAGCT+GGG | - | chr7.2:79366600-79366619 | None:intergenic | 35.0% | |
| TGTGTATGAGCTTAATGAGA+GGG | + | chr7.2:79366564-79366583 | MS.gene43207:CDS | 35.0% | |
| TTGCTTAATCTCAAGTAAGC+TGG | - | chr7.2:79366601-79366620 | None:intergenic | 35.0% | |
| ! | CTTTCTTTTCTGCAATGTGT+TGG | - | chr7.2:79367140-79367159 | None:intergenic | 35.0% |
| ! | TGTCAATTCTCTTGGTGATT+TGG | + | chr7.2:79366627-79366646 | MS.gene43207:CDS | 35.0% |
| GGTGACCTACAAAAACGTAT+TGG | + | chr7.2:79367087-79367106 | MS.gene43207:CDS | 40.0% | |
| TAGATAAGTGTCCTCAGGAT+AGG | - | chr7.2:79367238-79367257 | None:intergenic | 40.0% | |
| TATGGTCACTTAGCTGTTCA+AGG | + | chr7.2:79367201-79367220 | MS.gene43207:CDS | 40.0% | |
| TCTCAAAGCATAGCTTGAAG+AGG | - | chr7.2:79366212-79366231 | None:intergenic | 40.0% | |
| ! | GCTGTCTACAGTTTCCATTT+TGG | + | chr7.2:79367174-79367193 | MS.gene43207:CDS | 40.0% |
| !!! | CACACAATCTAGCTTTTCTG+CGG | + | chr7.2:79366369-79366388 | MS.gene43207:CDS | 40.0% |
| !!! | GGTGGTTCTGGAATTTTTGA+AGG | + | chr7.2:79367267-79367286 | MS.gene43207:CDS | 40.0% |
| AAACCAGCAATGACAGCATG+AGG | - | chr7.2:79367453-79367472 | None:intergenic | 45.0% | |
| ACAGCTAGATAAGTGTCCTC+AGG | - | chr7.2:79367243-79367262 | None:intergenic | 45.0% | |
| ACTTATCTAGCTGTGACTGG+TGG | + | chr7.2:79367249-79367268 | MS.gene43207:CDS | 45.0% | |
| AGCCAAGCAAATGAGTATGC+TGG | + | chr7.2:79366418-79366437 | MS.gene43207:CDS | 45.0% | |
| GACACTTATCTAGCTGTGAC+TGG | + | chr7.2:79367246-79367265 | MS.gene43207:CDS | 45.0% | |
| GATTTGCCTCAAGAGCTTCT+TGG | + | chr7.2:79367369-79367388 | MS.gene43207:CDS | 45.0% | |
| GGTTTACCAAGAAGCTCTTG+AGG | - | chr7.2:79367378-79367397 | None:intergenic | 45.0% | |
| TGCCAGCATACTCATTTGCT+TGG | - | chr7.2:79366423-79366442 | None:intergenic | 45.0% | |
| ! | AAGGGTGTGAAAGCAGATGA+GGG | - | chr7.2:79366298-79366317 | None:intergenic | 45.0% |
| !!! | ACAATCTAGCTTTTCTGCGG+TGG | + | chr7.2:79366372-79366391 | MS.gene43207:CDS | 45.0% |
| !!! | TAAAATTTTCAAATAATTTA+AGG | + | chr7.2:79366897-79366916 | MS.gene43207:intron | 5.0% |
| AGGGTGTGAAAGCAGATGAG+GGG | - | chr7.2:79366297-79366316 | None:intergenic | 50.0% | |
| GAAGCTTCAACAGCAGGAGA+AGG | - | chr7.2:79367408-79367427 | None:intergenic | 50.0% | |
| GCAAATGAGTATGCTGGCAC+TGG | + | chr7.2:79366424-79366443 | MS.gene43207:CDS | 50.0% | |
| TCAAGTAAGCTGGGCTACCA+CGG | - | chr7.2:79366591-79366610 | None:intergenic | 50.0% | |
| ! | AGGGGGTGAGGAAGGATTTT+GGG | - | chr7.2:79366279-79366298 | None:intergenic | 50.0% |
| ! | GAAGGGTGTGAAAGCAGATG+AGG | - | chr7.2:79366299-79366318 | None:intergenic | 50.0% |
| ACAGCAGGAGAAGGCTCAAC+AGG | - | chr7.2:79367399-79367418 | None:intergenic | 55.0% | |
| CTAGCTGTGACTGGTGGTTC+TGG | + | chr7.2:79367255-79367274 | MS.gene43207:CDS | 55.0% | |
| GAGCCTCATGCTGTCATTGC+TGG | + | chr7.2:79367447-79367466 | MS.gene43207:CDS | 55.0% | |
| GAGCTTAATGAGAGGGACCG+TGG | + | chr7.2:79366571-79366590 | MS.gene43207:CDS | 55.0% | |
| GCAGGAGAAGCTTCAACAGC+AGG | - | chr7.2:79367414-79367433 | None:intergenic | 55.0% | |
| GGCTCAAGAGACTTAGCAGC+AGG | - | chr7.2:79367432-79367451 | None:intergenic | 55.0% | |
| GGGTGTGAAAGCAGATGAGG+GGG | - | chr7.2:79366296-79366315 | None:intergenic | 55.0% | |
| TGAAAGCAGATGAGGGGGTG+AGG | - | chr7.2:79366291-79366310 | None:intergenic | 55.0% | |
| ! | GAGGGGGTGAGGAAGGATTT+TGG | - | chr7.2:79366280-79366299 | None:intergenic | 55.0% |
| ! | GGGGGTGAGGAAGGATTTTG+GGG | - | chr7.2:79366278-79366297 | None:intergenic | 55.0% |
| !! | GGTGAGGAAGGATTTTGGGG+TGG | - | chr7.2:79366275-79366294 | None:intergenic | 55.0% |
| !!! | GAGGAAGGATTTTGGGGTGG+TGG | - | chr7.2:79366272-79366291 | None:intergenic | 55.0% |
| AGCAGATGAGGGGGTGAGGA+AGG | - | chr7.2:79366287-79366306 | None:intergenic | 60.0% | |
| ! | GGGGTGGTGGCAAAAGCAGT+TGG | - | chr7.2:79366259-79366278 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 79366205 | 79367482 | 79366205 | ID=MS.gene43207 |
| chr7.2 | mRNA | 79366205 | 79367482 | 79366205 | ID=MS.gene43207.t1;Parent=MS.gene43207 |
| chr7.2 | exon | 79366205 | 79366445 | 79366205 | ID=MS.gene43207.t1.exon1;Parent=MS.gene43207.t1 |
| chr7.2 | CDS | 79366205 | 79366445 | 79366205 | ID=cds.MS.gene43207.t1;Parent=MS.gene43207.t1 |
| chr7.2 | exon | 79366545 | 79366666 | 79366545 | ID=MS.gene43207.t1.exon2;Parent=MS.gene43207.t1 |
| chr7.2 | CDS | 79366545 | 79366666 | 79366545 | ID=cds.MS.gene43207.t1;Parent=MS.gene43207.t1 |
| chr7.2 | exon | 79367078 | 79367482 | 79367078 | ID=MS.gene43207.t1.exon3;Parent=MS.gene43207.t1 |
| chr7.2 | CDS | 79367078 | 79367482 | 79367078 | ID=cds.MS.gene43207.t1;Parent=MS.gene43207.t1 |
| Gene Sequence |
| Protein sequence |
