Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43444.t1 | XP_013458643.1 | 58.1 | 172 | 34 | 3 | 1 | 148 | 1 | 158 | 8.60E-29 | 136.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43444.t1 | A0A072V3D4 | 58.1 | 172 | 34 | 3 | 1 | 148 | 1 | 158 | 6.2e-29 | 136.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053396 | MS.gene43444 | 0.818617 | 1.79E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43444.t1 | MTR_4g133515 | 64.024 | 164 | 23 | 2 | 1 | 142 | 1 | 150 | 4.76e-65 | 195 |
| MS.gene43444.t1 | MTR_4g133515 | 56.000 | 100 | 8 | 2 | 1 | 78 | 1 | 86 | 2.09e-27 | 99.0 |
| MS.gene43444.t1 | MTR_4g133360 | 73.016 | 63 | 17 | 0 | 80 | 142 | 1 | 63 | 2.13e-24 | 89.7 |
| MS.gene43444.t1 | MTR_4g133360 | 69.091 | 55 | 17 | 0 | 88 | 142 | 1 | 55 | 1.52e-18 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAAAGACTTCAGAGAAAA+TGG | 0.256513 | 8.4:+632388 | MS.gene43444:CDS |
| ACAGGATAAATATGAAAATA+TGG | 0.284798 | 8.4:+632136 | MS.gene43444:intron |
| AGCTGGAAGCTTTGCTTGCT+CGG | 0.347069 | 8.4:+632354 | MS.gene43444:intron |
| TATCATTATTAACAAGCATC+AGG | 0.412703 | 8.4:-632232 | None:intergenic |
| GATGCTGAAGACATAGTAAC+TGG | 0.433214 | 8.4:+631772 | MS.gene43444:CDS |
| CAAGTTCTGATAACACGAAT+AGG | 0.451357 | 8.4:+631731 | MS.gene43444:CDS |
| CTATTCGTGTTATCAGAACT+TGG | 0.465198 | 8.4:-631730 | None:intergenic |
| TAAATATGAAAATATGGAAA+AGG | 0.473598 | 8.4:+632142 | MS.gene43444:CDS |
| TTACATGCAAGAAGAGGAAA+AGG | 0.487878 | 8.4:+632424 | MS.gene43444:CDS |
| CAGAACAGCTGATGAGTTGA+GGG | 0.507301 | 8.4:+632043 | MS.gene43444:CDS |
| TTATTAACTTAGGAAAACCT+TGG | 0.510321 | 8.4:+631919 | MS.gene43444:intron |
| CGGATGAAATGAAATGTGAC+TGG | 0.533408 | 8.4:+631976 | MS.gene43444:CDS |
| AAGTACTCTTATATATTCGC+AGG | 0.548263 | 8.4:+632020 | MS.gene43444:CDS |
| ACTGGAGGAAAGAGGAATGA+AGG | 0.555180 | 8.4:+631790 | MS.gene43444:CDS |
| GCAGAACAGCTGATGAGTTG+AGG | 0.555622 | 8.4:+632042 | MS.gene43444:CDS |
| ACATAGTAACTGGAGGAAAG+AGG | 0.562414 | 8.4:+631782 | MS.gene43444:CDS |
| AAATGAAATGTGACTGGGCA+AGG | 0.566618 | 8.4:+631982 | MS.gene43444:CDS |
| GCATCGGAAGTAGTCTTCAC+AGG | 0.570061 | 8.4:-631754 | None:intergenic |
| AGTTACTATGTCTTCAGCAT+CGG | 0.572868 | 8.4:-631770 | None:intergenic |
| AGTTAATTACATGCAAGAAG+AGG | 0.578388 | 8.4:+632418 | MS.gene43444:CDS |
| TATTCATCAGATCGCATCCA+AGG | 0.582787 | 8.4:-631936 | None:intergenic |
| ATGGAAGAAAGCTCAAGAGA+TGG | 0.623297 | 8.4:+632209 | MS.gene43444:CDS |
| GCTGAAGACATAGTAACTGG+AGG | 0.677497 | 8.4:+631775 | MS.gene43444:CDS |
| ATACGTATTGATCAAAGCTG+CGG | 0.682966 | 8.4:+631956 | MS.gene43444:CDS |
| GGATGAAATGAAATGTGACT+GGG | 0.699158 | 8.4:+631977 | MS.gene43444:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACATAATACTAAATAT+AGG | - | chr8.4:632290-632309 | None:intergenic | 10.0% |
| !! | TAATAATGATAAAAACAAAA+TGG | + | chr8.4:632244-632263 | MS.gene43444:CDS | 10.0% |
| !! | TTGAATAAAATTGAATTAAA+TGG | + | chr8.4:632308-632327 | MS.gene43444:intron | 10.0% |
| !! | TAAATATGAAAATATGGAAA+AGG | + | chr8.4:632142-632161 | MS.gene43444:CDS | 15.0% |
| !! | AACATAATACTAAATATAGG+TGG | - | chr8.4:632287-632306 | None:intergenic | 20.0% |
| !! | ACAGGATAAATATGAAAATA+TGG | + | chr8.4:632136-632155 | MS.gene43444:intron | 20.0% |
| !!! | GAATATATAAGAGTACTTTT+TGG | - | chr8.4:632018-632037 | None:intergenic | 20.0% |
| ! | CACATACATGTTATTAACTT+AGG | + | chr8.4:631909-631928 | MS.gene43444:intron | 25.0% |
| ! | TATCATTATTAACAAGCATC+AGG | - | chr8.4:632235-632254 | None:intergenic | 25.0% |
| ! | TTATTAACTTAGGAAAACCT+TGG | + | chr8.4:631919-631938 | MS.gene43444:intron | 25.0% |
| AAGTACTCTTATATATTCGC+AGG | + | chr8.4:632020-632039 | MS.gene43444:CDS | 30.0% | |
| AGTTAATTACATGCAAGAAG+AGG | + | chr8.4:632418-632437 | MS.gene43444:CDS | 30.0% | |
| ATACAATACGTACAAAGACT+AGG | - | chr8.4:632094-632113 | None:intergenic | 30.0% | |
| ATTGTCTTCAAATTGCATAC+AGG | + | chr8.4:632118-632137 | MS.gene43444:intron | 30.0% | |
| TGCAAAAAAGAAACAACAGA+TGG | + | chr8.4:632190-632209 | MS.gene43444:CDS | 30.0% | |
| ! | ATATGATGCTTTGTTGTATG+TGG | + | chr8.4:631834-631853 | MS.gene43444:intron | 30.0% |
| ! | TTTTTCTTTTTACTTCCAGC+TGG | + | chr8.4:632337-632356 | MS.gene43444:intron | 30.0% |
| AGTTACTATGTCTTCAGCAT+CGG | - | chr8.4:631773-631792 | None:intergenic | 35.0% | |
| CTATTCGTGTTATCAGAACT+TGG | - | chr8.4:631733-631752 | None:intergenic | 35.0% | |
| GAGAAAGACTTCAGAGAAAA+TGG | + | chr8.4:632388-632407 | MS.gene43444:CDS | 35.0% | |
| GGATGAAATGAAATGTGACT+GGG | + | chr8.4:631977-631996 | MS.gene43444:CDS | 35.0% | |
| GTATGTGGTATGCTAATCAA+TGG | + | chr8.4:631849-631868 | MS.gene43444:intron | 35.0% | |
| TTACATGCAAGAAGAGGAAA+AGG | + | chr8.4:632424-632443 | MS.gene43444:CDS | 35.0% | |
| ! | ATACGTATTGATCAAAGCTG+CGG | + | chr8.4:631956-631975 | MS.gene43444:CDS | 35.0% |
| ! | CAAGTTCTGATAACACGAAT+AGG | + | chr8.4:631731-631750 | MS.gene43444:CDS | 35.0% |
| AAATGAAATGTGACTGGGCA+AGG | + | chr8.4:631982-632001 | MS.gene43444:CDS | 40.0% | |
| ACATAGTAACTGGAGGAAAG+AGG | + | chr8.4:631782-631801 | MS.gene43444:CDS | 40.0% | |
| ATGGAAGAAAGCTCAAGAGA+TGG | + | chr8.4:632209-632228 | MS.gene43444:CDS | 40.0% | |
| CGGATGAAATGAAATGTGAC+TGG | + | chr8.4:631976-631995 | MS.gene43444:CDS | 40.0% | |
| GATGCTGAAGACATAGTAAC+TGG | + | chr8.4:631772-631791 | MS.gene43444:CDS | 40.0% | |
| TATTCATCAGATCGCATCCA+AGG | - | chr8.4:631939-631958 | None:intergenic | 40.0% | |
| ACTGGAGGAAAGAGGAATGA+AGG | + | chr8.4:631790-631809 | MS.gene43444:CDS | 45.0% | |
| GCTGAAGACATAGTAACTGG+AGG | + | chr8.4:631775-631794 | MS.gene43444:CDS | 45.0% | |
| ! | CAGAACAGCTGATGAGTTGA+GGG | + | chr8.4:632043-632062 | MS.gene43444:CDS | 45.0% |
| AGCTGGAAGCTTTGCTTGCT+CGG | + | chr8.4:632354-632373 | MS.gene43444:intron | 50.0% | |
| GCAGAACAGCTGATGAGTTG+AGG | + | chr8.4:632042-632061 | MS.gene43444:CDS | 50.0% | |
| GCATCGGAAGTAGTCTTCAC+AGG | - | chr8.4:631757-631776 | None:intergenic | 50.0% | |
| GAGCAAGCAAAGCTTCCAGC+TGG | - | chr8.4:632355-632374 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 631724 | 632454 | 631724 | ID=MS.gene43444 |
| chr8.4 | mRNA | 631724 | 632454 | 631724 | ID=MS.gene43444.t1;Parent=MS.gene43444 |
| chr8.4 | exon | 631724 | 631811 | 631724 | ID=MS.gene43444.t1.exon1;Parent=MS.gene43444.t1 |
| chr8.4 | CDS | 631724 | 631811 | 631724 | ID=cds.MS.gene43444.t1;Parent=MS.gene43444.t1 |
| chr8.4 | exon | 631931 | 632064 | 631931 | ID=MS.gene43444.t1.exon2;Parent=MS.gene43444.t1 |
| chr8.4 | CDS | 631931 | 632064 | 631931 | ID=cds.MS.gene43444.t1;Parent=MS.gene43444.t1 |
| chr8.4 | exon | 632140 | 632265 | 632140 | ID=MS.gene43444.t1.exon3;Parent=MS.gene43444.t1 |
| chr8.4 | CDS | 632140 | 632265 | 632140 | ID=cds.MS.gene43444.t1;Parent=MS.gene43444.t1 |
| chr8.4 | exon | 632356 | 632454 | 632356 | ID=MS.gene43444.t1.exon4;Parent=MS.gene43444.t1 |
| chr8.4 | CDS | 632356 | 632454 | 632356 | ID=cds.MS.gene43444.t1;Parent=MS.gene43444.t1 |
| Gene Sequence |
| Protein sequence |
