Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43754.t1 | XP_003615573.1 | 99.4 | 161 | 1 | 0 | 1 | 161 | 1 | 161 | 9.50E-90 | 339.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43754.t1 | G7KE40 | 99.4 | 161 | 1 | 0 | 1 | 161 | 1 | 161 | 6.8e-90 | 339.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057444 | MS.gene43754 | 0.800834 | 1.20E-48 | -1.69E-46 |
| MS.gene058037 | MS.gene43754 | 0.803003 | 4.29E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43754.t1 | MTR_5g069660 | 99.379 | 161 | 1 | 0 | 1 | 161 | 1 | 161 | 7.14e-118 | 330 |
| MS.gene43754.t1 | MTR_3g462700 | 46.012 | 163 | 70 | 5 | 4 | 160 | 4 | 154 | 1.89e-41 | 136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43754.t1 | AT5G58920 | 63.030 | 165 | 53 | 2 | 1 | 160 | 1 | 162 | 9.39e-74 | 218 |
Find 49 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGGAGGAGTTTATTAAAA+AGG | 0.222603 | 5.1:-54039007 | MS.gene43754:CDS |
| AGAAAGGTACGTACCTGTTT+TGG | 0.232087 | 5.1:+54039364 | None:intergenic |
| GCTTCCTCGTCTCCCGGATA+TGG | 0.255545 | 5.1:+54039030 | None:intergenic |
| TCATTCTCCACCATTCCTTT+AGG | 0.299774 | 5.1:+54038958 | None:intergenic |
| GGCCGAATAATTACTACTTC+TGG | 0.300525 | 5.1:-54039203 | MS.gene43754:CDS |
| TGAAGTTATTGGTGAGCTTC+AGG | 0.305838 | 5.1:-54038851 | MS.gene43754:CDS |
| TTCCAGAAGTAGTAATTATT+CGG | 0.314764 | 5.1:+54039201 | None:intergenic |
| TCTAGCTCTTTCTCTTCCTT+TGG | 0.322195 | 5.1:+54039078 | None:intergenic |
| TGATTATAAGAAGGAGCTTA+AGG | 0.365177 | 5.1:-54038923 | MS.gene43754:CDS |
| AGCAAGAGGCTCAGAAGCTT+TGG | 0.366979 | 5.1:-54038888 | MS.gene43754:CDS |
| AGGCTCAGAAGCTTTGGTTT+AGG | 0.390308 | 5.1:-54038882 | MS.gene43754:CDS |
| TACTACTTCTGGAAACAAAT+GGG | 0.395334 | 5.1:-54039192 | MS.gene43754:CDS |
| GGGATGATTGGAACAACTAT+TGG | 0.398836 | 5.1:-54038982 | MS.gene43754:CDS |
| AGGAATGGTGGAGAATGATA+TGG | 0.401580 | 5.1:-54038953 | MS.gene43754:CDS |
| TCTTTCTCTTCCTTTGGCAT+TGG | 0.402785 | 5.1:+54039084 | None:intergenic |
| TTACTACTTCTGGAAACAAA+TGG | 0.407229 | 5.1:-54039193 | MS.gene43754:CDS |
| AGGATGAGGAATGAAGTTAT+TGG | 0.417045 | 5.1:-54038862 | MS.gene43754:CDS |
| GAAACAAATGGGGAAGGCTT+TGG | 0.427981 | 5.1:-54039181 | MS.gene43754:CDS |
| GCGGCGTGAGTTTCCATATC+CGG | 0.458287 | 5.1:-54039043 | MS.gene43754:CDS |
| AGAAGCTTTGGTTTAGGATG+AGG | 0.464134 | 5.1:-54038876 | MS.gene43754:CDS |
| TTCAACGAGACGTCGCCATT+GGG | 0.472078 | 5.1:-54039123 | MS.gene43754:CDS |
| ATTCAACGAGACGTCGCCAT+TGG | 0.476874 | 5.1:-54039124 | MS.gene43754:CDS |
| TCCATAGCTTCCTCGTCTCC+CGG | 0.480393 | 5.1:+54039024 | None:intergenic |
| GGAACAACTATTGGACCTAA+AGG | 0.486723 | 5.1:-54038973 | MS.gene43754:CDS |
| GGATAAGAAGTTTGAGCAAG+AGG | 0.490071 | 5.1:-54038902 | MS.gene43754:CDS |
| TTTCTCAAGAAAATAGGGCT+GGG | 0.498400 | 5.1:-54039225 | MS.gene43754:CDS |
| TATTGGTGAGCTTCAGGAGA+AGG | 0.502673 | 5.1:-54038845 | MS.gene43754:CDS |
| GAAACACCAGAACCCAAAAC+AGG | 0.504693 | 5.1:-54039377 | MS.gene43754:intron |
| TCCGGGAGACGAGGAAGCTA+TGG | 0.505414 | 5.1:-54039025 | MS.gene43754:CDS |
| GGCGTGCACTTATGCAGTGA+TGG | 0.519711 | 5.1:-54039160 | MS.gene43754:CDS |
| AGACGTCGCCATTGGGTTGG+TGG | 0.529186 | 5.1:-54039116 | MS.gene43754:CDS |
| GGATGAGAGTGATTATAAGA+AGG | 0.532632 | 5.1:-54038932 | MS.gene43754:CDS |
| ATTGGTGAGCTTCAGGAGAA+GGG | 0.553628 | 5.1:-54038844 | MS.gene43754:CDS |
| CGGCGTGAGTTTCCATATCC+GGG | 0.557934 | 5.1:-54039042 | MS.gene43754:CDS |
| TTCTCAAGAAAATAGGGCTG+GGG | 0.558610 | 5.1:-54039224 | MS.gene43754:CDS |
| GCGTGCACTTATGCAGTGAT+GGG | 0.574227 | 5.1:-54039159 | MS.gene43754:CDS |
| ACGAGACGTCGCCATTGGGT+TGG | 0.576055 | 5.1:-54039119 | MS.gene43754:CDS |
| AGCTAGAACATTTGTATCAG+CGG | 0.577075 | 5.1:-54039062 | MS.gene43754:CDS |
| AACTATTGGACCTAAAGGAA+TGG | 0.579872 | 5.1:-54038968 | MS.gene43754:CDS |
| GTTTCGAAGAAGCGTTTACG+AGG | 0.598958 | 5.1:+54039395 | None:intergenic |
| TATTGGACCTAAAGGAATGG+TGG | 0.604856 | 5.1:-54038965 | MS.gene43754:CDS |
| GACGTTGAAGCCAATGCCAA+AGG | 0.604896 | 5.1:-54039094 | MS.gene43754:CDS |
| GGGAGACGAGGAAGCTATGG+AGG | 0.612133 | 5.1:-54039022 | MS.gene43754:CDS |
| CACTTATGCAGTGATGGGTG+CGG | 0.618073 | 5.1:-54039154 | MS.gene43754:CDS |
| TTCAACGTCCACCAACCCAA+TGG | 0.629360 | 5.1:+54039108 | None:intergenic |
| CTTCTGGAAACAAATGGGGA+AGG | 0.632900 | 5.1:-54039187 | MS.gene43754:CDS |
| ACTACTTCTGGAAACAAATG+GGG | 0.649071 | 5.1:-54039191 | MS.gene43754:CDS |
| GTTTCCATATCCGGGAGACG+AGG | 0.665144 | 5.1:-54039034 | MS.gene43754:CDS |
| ATGCAGTGATGGGTGCGGCG+TGG | 0.699663 | 5.1:-54039149 | MS.gene43754:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATAAAATGTAAAAAATTAT+TGG | + | chr5.1:54038954-54038973 | None:intergenic | 10.0% |
| !!! | AATATTTTCTCAAGAAAATA+GGG | - | chr5.1:54038993-54039012 | MS.gene43754:CDS | 15.0% |
| !!! | TATTTTCTTGAGAAAATATT+GGG | + | chr5.1:54038993-54039012 | None:intergenic | 15.0% |
| !!! | AATTTTTTACATTTTATGCC+AGG | - | chr5.1:54038956-54038975 | MS.gene43754:CDS | 20.0% |
| !!! | CAATATTTTCTCAAGAAAAT+AGG | - | chr5.1:54038992-54039011 | MS.gene43754:CDS | 20.0% |
| !!! | CTATTTTCTTGAGAAAATAT+TGG | + | chr5.1:54038994-54039013 | None:intergenic | 20.0% |
| ! | ATGGAGGAGTTTATTAAAAA+GGG | - | chr5.1:54039217-54039236 | MS.gene43754:CDS | 25.0% |
| ! | TATGGAGGAGTTTATTAAAA+AGG | - | chr5.1:54039216-54039235 | MS.gene43754:CDS | 25.0% |
| ! | TTCCAGAAGTAGTAATTATT+CGG | + | chr5.1:54039025-54039044 | None:intergenic | 25.0% |
| !! | GTGTAATAGTAAGTGTTTTA+CGG | + | chr5.1:54038890-54038909 | None:intergenic | 25.0% |
| TGATTATAAGAAGGAGCTTA+AGG | - | chr5.1:54039300-54039319 | MS.gene43754:intron | 30.0% | |
| TGGAGGAGTTTATTAAAAAG+GGG | - | chr5.1:54039218-54039237 | MS.gene43754:CDS | 30.0% | |
| ! | TACTACTTCTGGAAACAAAT+GGG | - | chr5.1:54039031-54039050 | MS.gene43754:CDS | 30.0% |
| ! | TTACTACTTCTGGAAACAAA+TGG | - | chr5.1:54039030-54039049 | MS.gene43754:CDS | 30.0% |
| AACTATTGGACCTAAAGGAA+TGG | - | chr5.1:54039255-54039274 | MS.gene43754:intron | 35.0% | |
| AGCTAGAACATTTGTATCAG+CGG | - | chr5.1:54039161-54039180 | MS.gene43754:CDS | 35.0% | |
| AGGAGTTTATTAAAAAGGGG+GGG | - | chr5.1:54039221-54039240 | MS.gene43754:CDS | 35.0% | |
| AGGATGAGGAATGAAGTTAT+TGG | - | chr5.1:54039361-54039380 | MS.gene43754:intron | 35.0% | |
| GAGGAGTTTATTAAAAAGGG+GGG | - | chr5.1:54039220-54039239 | MS.gene43754:CDS | 35.0% | |
| GGAGGAGTTTATTAAAAAGG+GGG | - | chr5.1:54039219-54039238 | MS.gene43754:CDS | 35.0% | |
| GGATGAGAGTGATTATAAGA+AGG | - | chr5.1:54039291-54039310 | MS.gene43754:intron | 35.0% | |
| TTTCTCAAGAAAATAGGGCT+GGG | - | chr5.1:54038998-54039017 | MS.gene43754:CDS | 35.0% | |
| ! | ACTACTTCTGGAAACAAATG+GGG | - | chr5.1:54039032-54039051 | MS.gene43754:CDS | 35.0% |
| ! | GTGTTTTACGGTAGTTAGAA+AGG | + | chr5.1:54038878-54038897 | None:intergenic | 35.0% |
| ! | TTTTCTCAAGAAAATAGGGC+TGG | - | chr5.1:54038997-54039016 | MS.gene43754:CDS | 35.0% |
| ACAATCCAACATTGCTCACA+AGG | - | chr5.1:54038908-54038927 | MS.gene43754:CDS | 40.0% | |
| ATTAAAAAGGGGGGGATGAT+TGG | - | chr5.1:54039229-54039248 | MS.gene43754:CDS | 40.0% | |
| GGAACAACTATTGGACCTAA+AGG | - | chr5.1:54039250-54039269 | MS.gene43754:intron | 40.0% | |
| GGATAAGAAGTTTGAGCAAG+AGG | - | chr5.1:54039321-54039340 | MS.gene43754:intron | 40.0% | |
| GGCCGAATAATTACTACTTC+TGG | - | chr5.1:54039020-54039039 | MS.gene43754:CDS | 40.0% | |
| GGGATGATTGGAACAACTAT+TGG | - | chr5.1:54039241-54039260 | MS.gene43754:CDS | 40.0% | |
| TATTGGACCTAAAGGAATGG+TGG | - | chr5.1:54039258-54039277 | MS.gene43754:intron | 40.0% | |
| TCATTCTCCACCATTCCTTT+AGG | + | chr5.1:54039268-54039287 | None:intergenic | 40.0% | |
| TCTTTCTCTTCCTTTGGCAT+TGG | + | chr5.1:54039142-54039161 | None:intergenic | 40.0% | |
| TGAAGTTATTGGTGAGCTTC+AGG | - | chr5.1:54039372-54039391 | MS.gene43754:intron | 40.0% | |
| TTCTCAAGAAAATAGGGCTG+GGG | - | chr5.1:54038999-54039018 | MS.gene43754:CDS | 40.0% | |
| ! | AGAAGCTTTGGTTTAGGATG+AGG | - | chr5.1:54039347-54039366 | MS.gene43754:intron | 40.0% |
| ! | AGGAATGGTGGAGAATGATA+TGG | - | chr5.1:54039270-54039289 | MS.gene43754:intron | 40.0% |
| ! | TATTGGGCATGTTCTAAACC+TGG | + | chr5.1:54038977-54038996 | None:intergenic | 40.0% |
| !! | AGAAAGGTACGTACCTGTTT+TGG | + | chr5.1:54038862-54038881 | None:intergenic | 40.0% |
| !! | GAAAGGTACGTACCTGTTTT+GGG | + | chr5.1:54038861-54038880 | None:intergenic | 40.0% |
| !! | TCTAGCTCTTTCTCTTCCTT+TGG | + | chr5.1:54039148-54039167 | None:intergenic | 40.0% |
| ATTGGTGAGCTTCAGGAGAA+GGG | - | chr5.1:54039379-54039398 | MS.gene43754:CDS | 45.0% | |
| GAAACAAATGGGGAAGGCTT+TGG | - | chr5.1:54039042-54039061 | MS.gene43754:CDS | 45.0% | |
| GAAACACCAGAACCCAAAAC+AGG | - | chr5.1:54038846-54038865 | MS.gene43754:CDS | 45.0% | |
| GAACACCTTGTGAGCAATGT+TGG | + | chr5.1:54038916-54038935 | None:intergenic | 45.0% | |
| TATTGGTGAGCTTCAGGAGA+AGG | - | chr5.1:54039378-54039397 | MS.gene43754:CDS | 45.0% | |
| ! | AGGCTCAGAAGCTTTGGTTT+AGG | - | chr5.1:54039341-54039360 | MS.gene43754:intron | 45.0% |
| ! | CTTCTGGAAACAAATGGGGA+AGG | - | chr5.1:54039036-54039055 | MS.gene43754:CDS | 45.0% |
| !! | GTTTCGAAGAAGCGTTTACG+AGG | + | chr5.1:54038831-54038850 | None:intergenic | 45.0% |
| !!! | TACGTACCTGTTTTGGGTTC+TGG | + | chr5.1:54038855-54038874 | None:intergenic | 45.0% |
| !! | ATAAAATGTAAAAAATTATT+GGG | + | chr5.1:54038953-54038972 | None:intergenic | 5.0% |
| AGCAAGAGGCTCAGAAGCTT+TGG | - | chr5.1:54039335-54039354 | MS.gene43754:intron | 50.0% | |
| ATTCAACGAGACGTCGCCAT+TGG | - | chr5.1:54039099-54039118 | MS.gene43754:CDS | 50.0% | |
| GACGTTGAAGCCAATGCCAA+AGG | - | chr5.1:54039129-54039148 | MS.gene43754:CDS | 50.0% | |
| GCGTGCACTTATGCAGTGAT+GGG | - | chr5.1:54039064-54039083 | MS.gene43754:CDS | 50.0% | |
| TTCAACGAGACGTCGCCATT+GGG | - | chr5.1:54039100-54039119 | MS.gene43754:CDS | 50.0% | |
| TTCAACGTCCACCAACCCAA+TGG | + | chr5.1:54039118-54039137 | None:intergenic | 50.0% | |
| !! | CACTTATGCAGTGATGGGTG+CGG | - | chr5.1:54039069-54039088 | MS.gene43754:CDS | 50.0% |
| CGGCGTGAGTTTCCATATCC+GGG | - | chr5.1:54039181-54039200 | MS.gene43754:CDS | 55.0% | |
| GCGGCGTGAGTTTCCATATC+CGG | - | chr5.1:54039180-54039199 | MS.gene43754:CDS | 55.0% | |
| GGCGTGCACTTATGCAGTGA+TGG | - | chr5.1:54039063-54039082 | MS.gene43754:CDS | 55.0% | |
| GTTTCCATATCCGGGAGACG+AGG | - | chr5.1:54039189-54039208 | MS.gene43754:CDS | 55.0% | |
| TCCATAGCTTCCTCGTCTCC+CGG | + | chr5.1:54039202-54039221 | None:intergenic | 55.0% | |
| ACGAGACGTCGCCATTGGGT+TGG | - | chr5.1:54039104-54039123 | MS.gene43754:CDS | 60.0% | |
| GCTTCCTCGTCTCCCGGATA+TGG | + | chr5.1:54039196-54039215 | None:intergenic | 60.0% | |
| GGGAGACGAGGAAGCTATGG+AGG | - | chr5.1:54039201-54039220 | MS.gene43754:CDS | 60.0% | |
| TCCGGGAGACGAGGAAGCTA+TGG | - | chr5.1:54039198-54039217 | MS.gene43754:CDS | 60.0% | |
| !! | AGACGTCGCCATTGGGTTGG+TGG | - | chr5.1:54039107-54039126 | MS.gene43754:CDS | 60.0% |
| !! | ATGCAGTGATGGGTGCGGCG+TGG | - | chr5.1:54039074-54039093 | MS.gene43754:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 54038825 | 54039420 | 54038825 | ID=MS.gene43754 |
| chr5.1 | mRNA | 54038825 | 54039420 | 54038825 | ID=MS.gene43754.t1;Parent=MS.gene43754 |
| chr5.1 | exon | 54039378 | 54039420 | 54039378 | ID=MS.gene43754.t1.exon1;Parent=MS.gene43754.t1 |
| chr5.1 | CDS | 54039378 | 54039420 | 54039378 | ID=cds.MS.gene43754.t1;Parent=MS.gene43754.t1 |
| chr5.1 | exon | 54038825 | 54039267 | 54038825 | ID=MS.gene43754.t1.exon2;Parent=MS.gene43754.t1 |
| chr5.1 | CDS | 54038825 | 54039267 | 54038825 | ID=cds.MS.gene43754.t1;Parent=MS.gene43754.t1 |
| Gene Sequence |
| Protein sequence |
