AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43909

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43909.t1 MTR_5g035150 95.402 435 20 0 1 435 1 435 0.0 849
MS.gene43909.t1 MTR_5g035070 65.287 435 149 2 1 435 5 437 0.0 567
MS.gene43909.t1 MTR_5g035090 62.299 435 158 3 1 435 3 431 0.0 550
MS.gene43909.t1 MTR_5g035080 66.667 405 115 6 34 435 37 424 0.0 540
MS.gene43909.t1 MTR_3g109840 61.009 436 143 9 1 433 6 417 0.0 524
MS.gene43909.t1 MTR_8g104970 56.710 462 150 9 16 432 10 466 0.0 516
MS.gene43909.t1 MTR_7g033230 61.972 355 128 4 82 433 261 611 5.32e-161 468
MS.gene43909.t1 MTR_7g033230 61.972 355 128 4 82 433 331 681 2.77e-160 468
MS.gene43909.t1 MTR_5g035140 64.906 265 86 4 172 433 7 267 3.59e-125 364
MS.gene43909.t1 MTR_8g099300 41.477 352 195 7 85 431 74 419 6.75e-94 289
MS.gene43909.t1 MTR_8g027900 40.227 353 201 5 83 431 209 555 8.70e-93 291
MS.gene43909.t1 MTR_6g016880 37.349 415 242 10 25 432 17 420 9.89e-93 287
MS.gene43909.t1 MTR_7g019120 34.625 413 248 10 29 432 22 421 3.05e-86 270
MS.gene43909.t1 MTR_6g007777 38.172 372 212 8 66 433 102 459 3.75e-83 263
MS.gene43909.t1 MTR_6g016875 38.275 371 215 7 68 432 47 409 1.09e-81 258
MS.gene43909.t1 MTR_2g016060 37.921 356 196 12 90 433 60 402 6.87e-76 242
MS.gene43909.t1 MTR_1g076690 33.609 363 214 11 85 433 223 572 1.11e-63 215
MS.gene43909.t1 MTR_6g061710 32.540 378 202 13 83 433 165 516 3.86e-62 210
MS.gene43909.t1 MTR_7g094750 31.956 363 216 12 84 433 90 434 2.05e-52 182
MS.gene43909.t1 MTR_2g022560 33.423 371 203 14 84 435 96 441 1.07e-48 172
MS.gene43909.t1 MTR_5g035120 49.419 172 76 3 4 175 5 165 9.65e-48 161
MS.gene43909.t1 MTR_0136s0010 31.967 366 209 14 85 433 170 512 1.57e-44 162
MS.gene43909.t1 MTR_4g126950 31.806 371 200 14 83 431 107 446 4.02e-44 160
MS.gene43909.t1 MTR_7g093450 29.976 417 238 17 43 433 202 590 1.09e-43 161
MS.gene43909.t1 MTR_5g059400 30.663 362 207 15 84 426 174 510 5.82e-43 158
MS.gene43909.t1 MTR_1g116130 30.663 362 207 15 84 426 174 510 5.82e-43 158
MS.gene43909.t1 MTR_4g016940 30.053 376 213 16 79 431 79 427 1.94e-42 155
MS.gene43909.t1 MTR_1g058380 29.111 371 217 14 83 433 53 397 5.63e-42 153
MS.gene43909.t1 MTR_4g125060 29.973 367 219 12 76 427 124 467 6.40e-42 154
MS.gene43909.t1 MTR_4g125060 29.973 367 219 12 76 427 163 506 9.45e-42 155
MS.gene43909.t1 MTR_8g093530 31.373 357 209 12 83 431 88 416 1.04e-41 153
MS.gene43909.t1 MTR_4g125060 29.973 367 219 12 76 427 192 535 1.49e-41 155
MS.gene43909.t1 MTR_7g109550 30.704 355 206 14 85 427 137 463 4.05e-41 152
MS.gene43909.t1 MTR_4g014440 31.111 360 213 16 85 427 62 403 4.26e-41 151
MS.gene43909.t1 MTR_8g069790 29.815 379 221 16 73 432 166 518 5.94e-41 153
MS.gene43909.t1 MTR_4g121560 27.842 431 244 18 27 431 64 453 1.68e-40 150
MS.gene43909.t1 MTR_5g071350 29.570 372 215 10 77 427 50 395 1.75e-40 149
MS.gene43909.t1 MTR_5g033800 27.989 368 227 12 81 433 141 485 7.69e-40 149
MS.gene43909.t1 MTR_7g068920 31.347 386 213 16 69 431 65 421 1.67e-39 147
MS.gene43909.t1 MTR_7g070060 30.495 364 210 16 85 427 64 405 1.16e-38 144
MS.gene43909.t1 MTR_8g463790 26.744 430 254 16 23 427 12 405 1.36e-38 144
MS.gene43909.t1 MTR_3g035830 29.167 360 196 10 83 427 29 344 2.78e-38 142
MS.gene43909.t1 MTR_3g464570 29.630 351 224 11 82 427 40 372 3.40e-38 142
MS.gene43909.t1 MTR_2g073510 31.549 355 207 12 82 431 30 353 4.15e-38 141
MS.gene43909.t1 MTR_3g090120 29.897 388 211 14 69 433 113 462 9.14e-37 140
MS.gene43909.t1 MTR_6g016240 28.125 448 263 18 8 431 4 416 7.16e-36 137
MS.gene43909.t1 MTR_3g021090 29.363 361 194 14 83 427 29 344 1.63e-35 135
MS.gene43909.t1 MTR_7g094750 32.661 248 148 7 84 321 90 328 3.17e-35 133
MS.gene43909.t1 MTR_3g070900 28.056 360 228 13 82 427 103 445 3.36e-35 135
MS.gene43909.t1 MTR_2g073500 29.330 358 207 14 82 431 26 345 4.78e-35 133
MS.gene43909.t1 MTR_3g021060 29.143 350 209 11 83 427 44 359 5.17e-35 133
MS.gene43909.t1 MTR_8g093870 26.203 374 219 11 83 431 131 472 9.41e-35 135
MS.gene43909.t1 MTR_3g049330 30.387 362 206 14 76 427 21 346 1.98e-34 131
MS.gene43909.t1 MTR_4g092830 27.934 426 238 17 41 431 1 392 9.54e-34 131
MS.gene43909.t1 MTR_2g091175 30.925 346 203 11 85 427 50 362 1.08e-33 130
MS.gene43909.t1 MTR_4g088030 30.029 343 207 10 84 424 32 343 2.57e-33 128
MS.gene43909.t1 MTR_5g083260 27.078 373 213 14 82 427 80 420 6.77e-33 129
MS.gene43909.t1 MTR_3g071650 28.022 364 215 13 85 427 89 426 2.46e-32 127
MS.gene43909.t1 MTR_4g079700 28.941 387 239 13 49 431 9 363 3.74e-32 125
MS.gene43909.t1 MTR_4g087730 28.986 345 208 10 84 424 44 355 4.14e-32 125
MS.gene43909.t1 MTR_1g050448 27.297 370 233 11 67 431 37 375 4.82e-32 125
MS.gene43909.t1 MTR_1g112210 26.891 357 220 13 80 427 54 378 5.73e-31 122
MS.gene43909.t1 MTR_2g015720 29.235 366 219 14 70 431 29 358 6.60e-31 122
MS.gene43909.t1 MTR_5g082880 27.624 362 228 13 83 427 163 507 1.30e-30 124
MS.gene43909.t1 MTR_5g034810 29.915 351 205 13 83 427 33 348 2.91e-30 120
MS.gene43909.t1 MTR_1g112210 25.714 350 220 11 80 427 54 365 3.55e-29 117
MS.gene43909.t1 MTR_4g014430 27.612 402 240 17 51 431 75 446 1.07e-28 117
MS.gene43909.t1 MTR_7g019140 36.424 151 87 3 29 174 22 168 3.39e-28 109
MS.gene43909.t1 MTR_5g033800 28.629 248 160 6 81 320 141 379 8.03e-27 111
MS.gene43909.t1 MTR_1g111010 27.397 365 216 15 73 431 20 341 1.14e-26 110
MS.gene43909.t1 MTR_7g070060 28.939 311 179 15 137 427 15 303 1.88e-23 100
MS.gene43909.t1 MTR_7g070060 30.488 246 146 9 85 319 64 295 5.69e-23 99.0
MS.gene43909.t1 MTR_4g102570 39.370 127 65 3 201 321 2 122 1.21e-21 91.3
MS.gene43909.t1 MTR_3g464570 27.817 284 182 11 149 427 3 268 2.85e-21 93.6
MS.gene43909.t1 MTR_7g117160 40.541 111 43 2 320 430 102 189 1.54e-18 83.6
MS.gene43909.t1 MTR_7g019130 29.600 125 81 3 206 326 4 125 3.78e-12 64.3
MS.gene43909.t1 MTR_0929s0010 30.000 110 68 3 29 133 22 127 6.89e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43909.t1 AT1G70230 48.113 424 188 8 27 435 9 415 1.57e-148 429
MS.gene43909.t1 AT5G15890 39.535 387 215 8 50 432 152 523 7.25e-98 303
MS.gene43909.t1 AT4G01080 38.539 397 223 7 35 429 51 428 2.35e-91 283
MS.gene43909.t1 AT4G23790 36.449 428 259 9 10 431 7 427 1.71e-90 281
MS.gene43909.t1 AT3G02440 38.764 356 197 7 85 432 127 469 4.69e-90 281
MS.gene43909.t1 AT3G02440 38.764 356 197 7 85 432 135 477 9.31e-90 281
MS.gene43909.t1 AT1G01430 38.624 378 217 8 61 431 78 447 2.57e-89 279
MS.gene43909.t1 AT3G28150 36.790 405 238 10 36 433 19 412 3.24e-89 277
MS.gene43909.t1 AT4G11090 36.343 432 254 12 9 431 6 425 6.80e-88 274
MS.gene43909.t1 AT5G15900 36.675 379 222 10 65 435 45 413 9.48e-81 256
MS.gene43909.t1 AT5G15900 36.675 379 222 10 65 435 64 432 1.40e-80 256
MS.gene43909.t1 AT4G25360 33.668 398 233 12 51 433 145 526 4.42e-69 228
MS.gene43909.t1 AT4G25360 33.668 398 233 12 51 433 145 526 4.42e-69 228
MS.gene43909.t1 AT5G51640 33.333 393 223 14 63 433 115 490 2.10e-65 218
MS.gene43909.t1 AT5G19160 34.332 367 210 11 84 435 98 448 8.80e-57 194
MS.gene43909.t1 AT3G06080 31.680 363 220 10 85 435 103 449 3.82e-52 182
MS.gene43909.t1 AT3G12060 32.249 369 207 15 77 427 188 531 1.69e-50 179
MS.gene43909.t1 AT5G06700 32.418 364 214 15 83 433 253 597 3.61e-46 168
MS.gene43909.t1 AT5G64020 30.811 370 215 14 85 431 57 408 9.20e-46 163
MS.gene43909.t1 AT1G48880 33.245 376 199 16 71 428 95 436 5.06e-45 162
MS.gene43909.t1 AT3G11570 31.143 350 194 14 101 431 98 419 4.22e-43 157
MS.gene43909.t1 AT2G14530 29.048 420 237 19 36 431 24 406 1.17e-42 155
MS.gene43909.t1 AT5G20590 31.250 368 215 13 75 427 131 475 2.54e-42 156
MS.gene43909.t1 AT5G49340 29.296 355 224 11 84 427 99 437 1.32e-40 150
MS.gene43909.t1 AT5G58600 28.796 382 218 11 68 431 55 400 4.28e-40 148
MS.gene43909.t1 AT3G14850 32.312 359 205 11 79 431 28 354 7.14e-40 146
MS.gene43909.t1 AT3G62390 28.532 361 226 13 83 433 136 474 7.58e-40 149
MS.gene43909.t1 AT5G06230 30.458 371 211 15 83 431 19 364 3.46e-39 145
MS.gene43909.t1 AT5G06230 30.458 371 211 15 83 431 60 405 5.80e-39 145
MS.gene43909.t1 AT2G31110 29.444 360 218 13 72 427 31 358 7.57e-38 141
MS.gene43909.t1 AT2G38320 28.453 362 230 12 80 427 54 400 9.20e-38 142
MS.gene43909.t1 AT2G30010 30.556 360 220 10 84 431 55 396 2.15e-37 140
MS.gene43909.t1 AT5G20680 27.204 397 245 11 57 431 83 457 2.83e-37 141
MS.gene43909.t1 AT2G30010 31.025 361 217 12 84 431 98 439 3.10e-37 141
MS.gene43909.t1 AT2G42570 31.285 358 195 15 85 431 47 364 3.65e-37 139
MS.gene43909.t1 AT5G19160 35.628 247 140 8 84 320 98 335 8.12e-37 138
MS.gene43909.t1 AT2G37720 29.076 368 216 12 83 431 141 482 9.93e-37 140
MS.gene43909.t1 AT5G20680 27.500 400 240 12 57 431 159 533 1.08e-36 141
MS.gene43909.t1 AT5G20680 27.500 400 240 12 57 431 177 551 1.10e-36 141
MS.gene43909.t1 AT5G20680 27.500 400 240 12 57 431 177 551 1.10e-36 141
MS.gene43909.t1 AT5G20680 27.500 400 240 12 57 431 177 551 1.10e-36 141
MS.gene43909.t1 AT5G20680 27.500 400 240 12 57 431 177 551 1.10e-36 141
MS.gene43909.t1 AT2G30900 29.261 352 213 11 82 427 40 361 3.23e-36 137
MS.gene43909.t1 AT1G73140 29.032 372 222 15 72 427 56 401 1.04e-35 136
MS.gene43909.t1 AT5G64470 27.855 359 228 12 85 431 55 394 3.28e-35 135
MS.gene43909.t1 AT1G60790 28.075 374 218 16 83 432 188 534 8.30e-35 136
MS.gene43909.t1 AT3G11030 27.374 358 230 11 83 427 104 444 2.39e-34 133
MS.gene43909.t1 AT2G34070 28.125 352 217 12 84 431 64 383 4.14e-34 131
MS.gene43909.t1 AT5G01360 27.701 361 227 13 83 428 34 375 5.21e-34 131
MS.gene43909.t1 AT1G29050 28.333 360 223 12 75 431 51 378 5.56e-34 131
MS.gene43909.t1 AT2G38320 28.834 326 204 11 115 427 7 317 6.31e-34 129
MS.gene43909.t1 AT5G64470 27.577 359 235 12 85 431 55 400 7.35e-34 131
MS.gene43909.t1 AT5G01360 27.701 361 227 13 83 428 90 431 1.97e-33 130
MS.gene43909.t1 AT2G40320 28.022 364 221 13 82 427 78 418 2.06e-33 130
MS.gene43909.t1 AT3G55990 28.177 362 223 13 83 427 138 479 2.99e-33 131
MS.gene43909.t1 AT2G40160 27.123 365 222 14 85 427 77 419 1.04e-32 128
MS.gene43909.t1 AT2G40160 27.123 365 222 14 85 427 77 419 1.30e-32 128
MS.gene43909.t1 AT3G06080 31.579 247 150 6 85 321 103 340 2.66e-32 125
MS.gene43909.t1 AT2G40150 28.165 387 232 16 63 427 54 416 4.67e-32 126
MS.gene43909.t1 AT1G78710 29.640 361 216 14 76 431 29 356 4.93e-31 122
MS.gene43909.t1 AT3G54260 27.222 360 213 14 85 431 51 374 3.37e-30 120
MS.gene43909.t1 AT5G01620 25.946 370 237 13 77 431 102 449 7.99e-28 115
MS.gene43909.t1 AT5G01620 25.946 370 237 13 77 431 102 449 7.99e-28 115
MS.gene43909.t1 AT5G01620 25.946 370 237 13 77 431 110 457 9.19e-28 115
MS.gene43909.t1 AT3G62390 28.455 246 163 6 83 322 136 374 2.70e-27 113
MS.gene43909.t1 AT5G20590 34.392 189 115 5 75 255 131 318 1.87e-26 110
MS.gene43909.t1 AT5G58600 30.038 263 145 8 68 319 55 289 1.84e-25 105
MS.gene43909.t1 AT5G06230 30.769 260 145 11 83 321 60 305 2.95e-24 102
MS.gene43909.t1 AT2G31110 32.609 184 116 4 72 254 31 207 4.18e-24 101
MS.gene43909.t1 AT2G40160 35.878 131 82 2 85 214 77 206 4.31e-23 99.4
MS.gene43909.t1 AT2G30900 33.714 175 109 3 82 255 40 208 1.30e-22 97.4
MS.gene43909.t1 AT5G64470 27.376 263 171 9 85 335 55 309 2.74e-19 88.6
MS.gene43909.t1 AT5G64470 27.376 263 171 9 85 335 55 309 3.75e-19 88.6
MS.gene43909.t1 AT3G14850 29.787 282 160 11 156 431 2 251 1.51e-18 85.5
MS.gene43909.t1 AT1G78710 31.667 180 114 4 76 254 22 193 2.93e-18 85.5
MS.gene43909.t1 AT2G34070 26.449 276 167 12 156 427 2 245 7.12e-16 77.4

Find 93 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTCAATTATACTATTA+AGG 0.109514 5.4:+24985269 None:intergenic
GTGCCACTTACGAAATTTAT+TGG 0.120096 5.4:+24985585 None:intergenic
CTGATTTAGGTTATCTTTAT+TGG 0.135262 5.4:-24985786 MS.gene43909:CDS
GTCCTCAAAGCCTTTGTTAA+TGG 0.201729 5.4:+24985295 None:intergenic
TATGAGAGTGATTTAGTTCT+TGG 0.213205 5.4:-24985367 MS.gene43909:CDS
TGCAAAGAAGTGACTCTAAT+TGG 0.285207 5.4:+24985656 None:intergenic
ACAAATGGTAGACCTGATTT+AGG 0.287971 5.4:-24985799 MS.gene43909:CDS
AGGTATTAGACTTGAGGTTT+TGG 0.334346 5.4:-24985041 MS.gene43909:CDS
TCTCTCAATTATACTATTAA+GGG 0.345275 5.4:+24985270 None:intergenic
GTGAATATTCAAATGGAGAT+TGG 0.348520 5.4:-24985894 MS.gene43909:CDS
AAAGTGTTTGGTTCAAATCT+TGG 0.349855 5.4:+24985736 None:intergenic
TCAACACCATGCACTAGAAA+TGG 0.356439 5.4:+24985523 None:intergenic
GAATGGATGGTGAGATTAGA+AGG 0.357080 5.4:-24985114 MS.gene43909:CDS
AGAAAAGTTGCTAAAGGAAA+TGG 0.366030 5.4:-24985250 MS.gene43909:CDS
TATAAACAAAGAAGGGATAT+AGG 0.366305 5.4:+24986246 None:intergenic
GACATGATTGTTATATCAAC+CGG 0.390990 5.4:-24985418 MS.gene43909:CDS
ATGTGGTGACATTGTATATA+GGG 0.397785 5.4:+24985854 None:intergenic
AGAAAAGAGGATAAGATAGT+TGG 0.399914 5.4:-24985139 MS.gene43909:CDS
ATTTATTGGACTTGCCATCT+TGG 0.403569 5.4:+24985599 None:intergenic
TTCCATTAACAAAGGCTTTG+AGG 0.412649 5.4:-24985297 MS.gene43909:CDS
CAACACCATGCACTAGAAAT+GGG 0.415330 5.4:+24985524 None:intergenic
TACAAAATGATTGTGTTCAT+TGG 0.415725 5.4:-24984928 MS.gene43909:CDS
TGTGTTCATTGGTGCTTGCC+AGG 0.419306 5.4:-24984917 MS.gene43909:CDS
CCATCTTGGTAAACAAGGTT+AGG 0.420926 5.4:+24985613 None:intergenic
GTTTGTTCTTAACGAGTTTG+AGG 0.427143 5.4:+24985713 None:intergenic
GGCAAAGGATATTGATCAAA+TGG 0.427874 5.4:-24985440 MS.gene43909:CDS
TTTGGAACTCCATTTGCAAA+TGG 0.431908 5.4:+24984956 None:intergenic
TGAATATTCAAATGGAGATT+GGG 0.433426 5.4:-24985893 MS.gene43909:CDS
TGAATAGTGGTAGGTGGAAA+TGG 0.434406 5.4:+24986167 None:intergenic
TCACTCTCATAGTAAATTGA+AGG 0.436067 5.4:+24985379 None:intergenic
GGATAAGATAGTTGGAGGAA+TGG 0.436305 5.4:-24985131 MS.gene43909:CDS
ACTCCATTTGCAAATGGAAA+AGG 0.436668 5.4:+24984962 None:intergenic
AAGAGTAAGTAGATGAATAG+TGG 0.440562 5.4:+24986154 None:intergenic
CATGTGGTGACATTGTATAT+AGG 0.444034 5.4:+24985853 None:intergenic
CAATAAAGATAACCTAAATC+AGG 0.445212 5.4:+24985787 None:intergenic
CTTGGTAGATTACACTCACT+TGG 0.451639 5.4:+24985754 None:intergenic
TAGGTTATCTTTATTGGAGA+TGG 0.460710 5.4:-24985780 MS.gene43909:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5.4:+24984992 None:intergenic
AGCAGCTTAGTTGGTGCAAA+AGG 0.466236 5.4:+24986201 None:intergenic
ACGAGTTTGAGGAAAGTGTT+TGG 0.469541 5.4:+24985724 None:intergenic
TAGTTGGTGCAAAAGGAGTA+AGG 0.469996 5.4:+24986208 None:intergenic
AAAGAGGATAAGATAGTTGG+AGG 0.471238 5.4:-24985136 MS.gene43909:CDS
TTGAGACCAGATGGTCATCC+AGG 0.473377 5.4:-24984998 MS.gene43909:CDS
TGTAGAGATGATGAAGAAGT+GGG 0.476890 5.4:-24984867 MS.gene43909:CDS
TGGTCCCCATTTCTAGTGCA+TGG 0.482297 5.4:-24985529 MS.gene43909:CDS
TGATGAAGTATAAACAAAGA+AGG 0.490126 5.4:+24986238 None:intergenic
GACTAAGCCTTATAGAAAAG+AGG 0.491810 5.4:-24985152 MS.gene43909:CDS
GTAAAAGCTAATAAATTTGA+AGG 0.492014 5.4:-24985061 MS.gene43909:CDS
CCTAACCTTGTTTACCAAGA+TGG 0.495701 5.4:-24985613 MS.gene43909:CDS
AATGGAAAAGGATTCATGTA+AGG 0.496598 5.4:+24984974 None:intergenic
AAATCATGTGAATATTCAAA+TGG 0.499467 5.4:-24985901 MS.gene43909:CDS
AAGATAGTTGGAGGAATGGA+TGG 0.499482 5.4:-24985127 MS.gene43909:CDS
GTAGAATGAAGCAGCTTAGT+TGG 0.499755 5.4:+24986192 None:intergenic
TTGTAGAGATGATGAAGAAG+TGG 0.501696 5.4:-24984868 MS.gene43909:CDS
TGCCAGGACCTATAGATACT+TGG 0.502047 5.4:-24984901 MS.gene43909:CDS
GGAGGAAAGATTGTCATTGA+AGG 0.503097 5.4:+24986311 None:intergenic
GTAAGTAGATGAATAGTGGT+AGG 0.506588 5.4:+24986158 None:intergenic
AGTGAGAGATGCATCACAAA+TGG 0.510170 5.4:-24985814 MS.gene43909:CDS
TTGTTGGAGATTCTTTGGCT+AGG 0.514286 5.4:-24985681 MS.gene43909:CDS
ATTGAGAGAAAAGTTGCTAA+AGG 0.514738 5.4:-24985256 MS.gene43909:CDS
GAAGGTGATTGGGATAAGGC+TGG 0.521359 5.4:-24985187 MS.gene43909:CDS
GGTGTTGAAAGATCAAATGA+AGG 0.522081 5.4:-24985508 MS.gene43909:CDS
ATCTCATTCCAAGTATCTAT+AGG 0.524335 5.4:+24984893 None:intergenic
GAGGAAAGATTGTCATTGAA+GGG 0.526115 5.4:+24986312 None:intergenic
GATGAAGTATAAACAAAGAA+GGG 0.527867 5.4:+24986239 None:intergenic
AGTCCAATAAATTTCGTAAG+TGG 0.528019 5.4:-24985588 MS.gene43909:CDS
TTGTTATATCAACCGGGCAT+TGG 0.533327 5.4:-24985411 MS.gene43909:CDS
TTCCAAGTATCTATAGGTCC+TGG 0.536833 5.4:+24984899 None:intergenic
ATTTGAAGGTATTAGACTTG+AGG 0.537007 5.4:-24985047 MS.gene43909:CDS
AAGGATTGTGATGGAAGAAG+TGG 0.538045 5.4:-24985095 MS.gene43909:CDS
ATACCTACAAAAGAAGTTGA+AGG 0.538773 5.4:+24986126 None:intergenic
GGATCTTGTCAATGAGAAGT+GGG 0.539423 5.4:-24985461 MS.gene43909:CDS
CAATCACCTTCAAAATGAGC+AGG 0.550833 5.4:+24985199 None:intergenic
TTCTTTGATTGTGCCACATG+TGG 0.561553 5.4:+24985837 None:intergenic
TGGATCTTGTCAATGAGAAG+TGG 0.565608 5.4:-24985462 MS.gene43909:CDS
GAAAATGTAGCATTATGAGA+AGG 0.570303 5.4:+24985559 None:intergenic
AGGAAAGATTGTCATTGAAG+GGG 0.571923 5.4:+24986313 None:intergenic
ATTTGAGTATGATTAAGCTC+AGG 0.573371 5.4:+24985331 None:intergenic
AGTAGATGAATAGTGGTAGG+TGG 0.577718 5.4:+24986161 None:intergenic
ACTTGCCATCTTGGTAAACA+AGG 0.587524 5.4:+24985608 None:intergenic
TTGGCATTATTGAGACCAGA+TGG 0.590154 5.4:-24985007 MS.gene43909:CDS
AAAGGATTCATGTAAGGACC+TGG 0.594011 5.4:+24984980 None:intergenic
TGTCAATGAGAAGTGGGCAA+AGG 0.618169 5.4:-24985455 MS.gene43909:CDS
TGAGATTAGAAGGATTGTGA+TGG 0.626027 5.4:-24985104 MS.gene43909:CDS
ATATACAATGTCACCACATG+TGG 0.627135 5.4:-24985850 MS.gene43909:CDS
GTGTTGAAAGATCAAATGAA+GGG 0.634222 5.4:-24985507 MS.gene43909:CDS
AGTTGGTGCAAAAGGAGTAA+GGG 0.640399 5.4:+24986209 None:intergenic
GGAAGAAGTGGAAAGTGCCA+AGG 0.647334 5.4:-24985083 MS.gene43909:CDS
CCTACAAAAGAAGTTGAAGG+AGG 0.659746 5.4:+24986129 None:intergenic
AACACCATGCACTAGAAATG+GGG 0.662494 5.4:+24985525 None:intergenic
ACATGATTGTTATATCAACC+GGG 0.662677 5.4:-24985417 MS.gene43909:CDS
GATTGGGTTAGTGACATGAG+AGG 0.690038 5.4:-24985877 MS.gene43909:CDS
ATAGTGGTAGGTGGAAATGG+TGG 0.690039 5.4:+24986170 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAATACAATGTTTT+TGG - chr5.4:24985707-24985726 MS.gene43909:CDS 10.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5.4:24984902-24984921 None:intergenic 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5.4:24985241-24985260 MS.gene43909:CDS 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5.4:24985240-24985259 MS.gene43909:CDS 15.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5.4:24984899-24984918 None:intergenic 20.0%
!! AAATCATGTGAATATTCAAA+TGG - chr5.4:24985288-24985307 MS.gene43909:CDS 20.0%
!! GTAAAAGCTAATAAATTTGA+AGG - chr5.4:24986128-24986147 MS.gene43909:intron 20.0%
!! TCTCTCAATTATACTATTAA+GGG + chr5.4:24985922-24985941 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTA+AGG + chr5.4:24985923-24985942 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTACCT+TGG + chr5.4:24986126-24986145 None:intergenic 20.0%
!!! CAATCATTTTGTACATATTT+TGG + chr5.4:24986254-24986273 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAACAA+AGG - chr5.4:24985884-24985903 MS.gene43909:CDS 20.0%
! CAATAAAGATAACCTAAATC+AGG + chr5.4:24985405-24985424 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5.4:24984953-24984972 None:intergenic 25.0%
! TACAAAATGATTGTGTTCAT+TGG - chr5.4:24986261-24986280 MS.gene43909:CDS 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5.4:24984946-24984965 None:intergenic 25.0%
! TGAATATTCAAATGGAGATT+GGG - chr5.4:24985296-24985315 MS.gene43909:CDS 25.0%
! TGATGAAGTATAAACAAAGA+AGG + chr5.4:24984954-24984973 None:intergenic 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5.4:24985492-24985511 MS.gene43909:CDS 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG + chr5.4:24986047-24986066 None:intergenic 25.0%
!! ATGCTACATTTTCTTTGTAT+TGG - chr5.4:24985640-24985659 MS.gene43909:CDS 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5.4:24985403-24985422 MS.gene43909:CDS 25.0%
!!! CTAGCAATTTGTTACTATTA+AGG - chr5.4:24985197-24985216 MS.gene43909:CDS 25.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5.4:24985038-24985057 None:intergenic 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5.4:24986218-24986237 None:intergenic 30.0%
ACATGATTGTTATATCAACC+GGG - chr5.4:24985772-24985791 MS.gene43909:CDS 30.0%
AGAAAAGAGGATAAGATAGT+TGG - chr5.4:24986050-24986069 MS.gene43909:intron 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5.4:24985939-24985958 MS.gene43909:intron 30.0%
AGTCCAATAAATTTCGTAAG+TGG - chr5.4:24985601-24985620 MS.gene43909:CDS 30.0%
ATACCTACAAAAGAAGTTGA+AGG + chr5.4:24985066-24985085 None:intergenic 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5.4:24986299-24986318 None:intergenic 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5.4:24985338-24985357 None:intergenic 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5.4:24985933-24985952 MS.gene43909:intron 30.0%
ATTTGAGTATGATTAAGCTC+AGG + chr5.4:24985861-24985880 None:intergenic 30.0%
GAAAATGTAGCATTATGAGA+AGG + chr5.4:24985633-24985652 None:intergenic 30.0%
GACATGATTGTTATATCAAC+CGG - chr5.4:24985771-24985790 MS.gene43909:CDS 30.0%
GTGAATATTCAAATGGAGAT+TGG - chr5.4:24985295-24985314 MS.gene43909:CDS 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5.4:24985682-24985701 MS.gene43909:CDS 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5.4:24985409-24985428 MS.gene43909:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5.4:24985813-24985832 None:intergenic 30.0%
TGTTACTATTAAGGACTTCT+TGG - chr5.4:24985206-24985225 MS.gene43909:CDS 30.0%
! AAAGTGTTTGGTTCAAATCT+TGG + chr5.4:24985456-24985475 None:intergenic 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5.4:24986224-24986243 MS.gene43909:CDS 30.0%
!! ATTTGAAGGTATTAGACTTG+AGG - chr5.4:24986142-24986161 MS.gene43909:CDS 30.0%
!! TATGAGAGTGATTTAGTTCT+TGG - chr5.4:24985822-24985841 MS.gene43909:CDS 30.0%
!!! GGTTTTGGATGTAACTAAAT+TGG - chr5.4:24986163-24986182 MS.gene43909:CDS 30.0%
AAAGAGGATAAGATAGTTGG+AGG - chr5.4:24986053-24986072 MS.gene43909:intron 35.0%
ACAAATGGTAGACCTGATTT+AGG - chr5.4:24985390-24985409 MS.gene43909:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5.4:24986230-24986249 None:intergenic 35.0%
AGGAAAGATTGTCATTGAAG+GGG + chr5.4:24984879-24984898 None:intergenic 35.0%
ATATACAATGTCACCACATG+TGG - chr5.4:24985339-24985358 MS.gene43909:CDS 35.0%
ATTTATTGGACTTGCCATCT+TGG + chr5.4:24985593-24985612 None:intergenic 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5.4:24985339-24985358 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG + chr5.4:24984880-24984899 None:intergenic 35.0%
GGCAAAGGATATTGATCAAA+TGG - chr5.4:24985749-24985768 MS.gene43909:CDS 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5.4:24985034-24985053 None:intergenic 35.0%
GTTTGTTCTTAACGAGTTTG+AGG + chr5.4:24985479-24985498 None:intergenic 35.0%
TGAGATTAGAAGGATTGTGA+TGG - chr5.4:24986085-24986104 MS.gene43909:intron 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5.4:24985536-24985555 None:intergenic 35.0%
TGTAGAGATGATGAAGAAGT+GGG - chr5.4:24986322-24986341 MS.gene43909:CDS 35.0%
TTCCATTAACAAAGGCTTTG+AGG - chr5.4:24985892-24985911 MS.gene43909:CDS 35.0%
TTGTAGAGATGATGAAGAAG+TGG - chr5.4:24986321-24986340 MS.gene43909:CDS 35.0%
! AGCTTTTGTTGGAGATTCTT+TGG - chr5.4:24985503-24985522 MS.gene43909:CDS 35.0%
! AGGTATTAGACTTGAGGTTT+TGG - chr5.4:24986148-24986167 MS.gene43909:CDS 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5.4:24986037-24986056 MS.gene43909:intron 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5.4:24985681-24985700 MS.gene43909:CDS 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5.4:24986236-24986255 None:intergenic 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5.4:24985984-24986003 MS.gene43909:intron 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5.4:24985607-24985626 None:intergenic 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5.4:24985992-24986011 MS.gene43909:intron 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5.4:24985998-24986017 MS.gene43909:intron 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG - chr5.4:24985251-24985270 MS.gene43909:CDS 35.0%
AAAGGATTCATGTAAGGACC+TGG + chr5.4:24986212-24986231 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5.4:24985667-24985686 None:intergenic 40.0%
AAGATAGTTGGAGGAATGGA+TGG - chr5.4:24986062-24986081 MS.gene43909:intron 40.0%
ACTTGCCATCTTGGTAAACA+AGG + chr5.4:24985584-24985603 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5.4:24985031-24985050 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5.4:24985375-24985394 MS.gene43909:CDS 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5.4:24985668-24985687 None:intergenic 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5.4:24985993-24986012 None:intergenic 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5.4:24985579-24985598 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5.4:24985063-24985082 None:intergenic 40.0%
CTTGGTAGATTACACTCACT+TGG + chr5.4:24985438-24985457 None:intergenic 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5.4:24986075-24986094 MS.gene43909:intron 40.0%
GGAGGAAAGATTGTCATTGA+AGG + chr5.4:24984881-24984900 None:intergenic 40.0%
GGATAAGATAGTTGGAGGAA+TGG - chr5.4:24986058-24986077 MS.gene43909:intron 40.0%
GGATCTTGTCAATGAGAAGT+GGG - chr5.4:24985728-24985747 MS.gene43909:CDS 40.0%
GTAGAATGAAGCAGCTTAGT+TGG + chr5.4:24985000-24985019 None:intergenic 40.0%
GTCCTCAAAGCCTTTGTTAA+TGG + chr5.4:24985897-24985916 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5.4:24985669-24985688 None:intergenic 40.0%
TGAATAGTGGTAGGTGGAAA+TGG + chr5.4:24985025-24985044 None:intergenic 40.0%
TGGATCTTGTCAATGAGAAG+TGG - chr5.4:24985727-24985746 MS.gene43909:CDS 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5.4:24986293-24986312 None:intergenic 40.0%
TTCTTTGATTGTGCCACATG+TGG + chr5.4:24985355-24985374 None:intergenic 40.0%
TTGGCATTATTGAGACCAGA+TGG - chr5.4:24986182-24986201 MS.gene43909:CDS 40.0%
TTGTTATATCAACCGGGCAT+TGG - chr5.4:24985778-24985797 MS.gene43909:CDS 40.0%
TTGTTGGAGATTCTTTGGCT+AGG - chr5.4:24985508-24985527 MS.gene43909:CDS 40.0%
! AAGGATTGTGATGGAAGAAG+TGG - chr5.4:24986094-24986113 MS.gene43909:intron 40.0%
! AGTTGGTGCAAAAGGAGTAA+GGG + chr5.4:24984983-24985002 None:intergenic 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5.4:24985576-24985595 MS.gene43909:CDS 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5.4:24985060-24985079 MS.gene43909:CDS 40.0%
! TAGTTGGTGCAAAAGGAGTA+AGG + chr5.4:24984984-24985003 None:intergenic 40.0%
!! ACGAGTTTGAGGAAAGTGTT+TGG + chr5.4:24985468-24985487 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5.4:24985991-24986010 MS.gene43909:intron 40.0%
AGGAAGCAAAAACCAATGCC+CGG + chr5.4:24985793-24985812 None:intergenic 45.0%
ATAGTGGTAGGTGGAAATGG+TGG + chr5.4:24985022-24985041 None:intergenic 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5.4:24986288-24986307 MS.gene43909:CDS 45.0%
TGTCAATGAGAAGTGGGCAA+AGG - chr5.4:24985734-24985753 MS.gene43909:CDS 45.0%
! AGCAGCTTAGTTGGTGCAAA+AGG + chr5.4:24984991-24985010 None:intergenic 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5.4:24985312-24985331 MS.gene43909:CDS 45.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5.4:24986002-24986021 MS.gene43909:intron 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5.4:24986200-24986219 None:intergenic 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5.4:24986191-24986210 MS.gene43909:CDS 50.0%
! TGTGTTCATTGGTGCTTGCC+AGG - chr5.4:24986272-24986291 MS.gene43909:CDS 50.0%
!! GGAAGAAGTGGAAAGTGCCA+AGG - chr5.4:24986106-24986125 MS.gene43909:intron 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5.4:24985660-24985679 MS.gene43909:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 24984862 24986349 24984862 ID=MS.gene43909
chr5.4 mRNA 24984862 24986349 24984862 ID=MS.gene43909.t1;Parent=MS.gene43909
chr5.4 exon 24986130 24986349 24986130 ID=MS.gene43909.t1.exon1;Parent=MS.gene43909.t1
chr5.4 CDS 24986130 24986349 24986130 ID=cds.MS.gene43909.t1;Parent=MS.gene43909.t1
chr5.4 exon 24984862 24985949 24984862 ID=MS.gene43909.t1.exon2;Parent=MS.gene43909.t1
chr5.4 CDS 24984862 24985949 24984862 ID=cds.MS.gene43909.t1;Parent=MS.gene43909.t1
Gene Sequence

>MS.gene43909

ATGGGAAGCACAAACCCCTTCAATGACAATCTTTCCTCCTTCAATAAGAAATTTTTTCATTTGACTCTTTATTCATTGCTTCCTATATCCCTTCTTTGTTTATACTTCATCATTTTGTCCCTTACTCCTTTTGCACCAACTAAGCTGCTTCATTCTACCACCATTTCCACCTACCACTATTCATCTACTTACTCTTCTCCTCCTTCAACTTCTTTTGTAGGTATATGCATGCATAATTAACTTCTAGTTGAGATTCACATGAACTCTAACTTCTATTCATCTACTTTTTTTTTAAAAAAAAAAAATATGCTAGTTGAGATTCACATGAACTCTAACTAGCAATTTGTTACTATTAAGGACTTCTTGGTTTACTAATTTTTATGTAATATATTTTTTGCAGGGAAGAACAAGGTTTATGATTATGAGAAATCATGTGAATATTCAAATGGAGATTGGGTTAGTGACATGAGAGGCCCTATATACAATGTCACCACATGTGGCACAATCAAAGAAAGTGAGAGATGCATCACAAATGGTAGACCTGATTTAGGTTATCTTTATTGGAGATGGAAGCCAAGTGAGTGTAATCTACCAAGATTTGAACCAAACACTTTCCTCAAACTCGTTAAGAACAAACATATAGCTTTTGTTGGAGATTCTTTGGCTAGGAACCAATTAGAGTCACTTCTTTGCATGTTAGCGACTACTTCAACACCTAACCTTGTTTACCAAGATGGCAAGTCCAATAAATTTCGTAAGTGGCACTTTCCTTCTCATAATGCTACATTTTCTTTGTATTGGTCCCCATTTCTAGTGCATGGTGTTGAAAGATCAAATGAAGGGAATTATTATAATACAATGTTTTTGGATCTTGTCAATGAGAAGTGGGCAAAGGATATTGATCAAATGGACATGATTGTTATATCAACCGGGCATTGGTTTTTGCTTCCTTCAATTTACTATGAGAGTGATTTAGTTCTTGGTAGCTTGAATTGTCCTGAGCTTAATCATACTCAAATTGATTTTTATGTTCCATTAACAAAGGCTTTGAGGACTACCCTTAATAGTATAATTGAGAGAAAAGTTGCTAAAGGAAATGGAATTGATGTGATTGTGAAAACATTTTCACCTGCTCATTTTGAAGGTGATTGGGATAAGGCTGGTACTTGTTCAAAGACTAAGCCTTATAGAAAAGAGGATAAGATAGTTGGAGGAATGGATGGTGAGATTAGAAGGATTGTGATGGAAGAAGTGGAAAGTGCCAAGGTAAAAGCTAATAAATTTGAAGGTATTAGACTTGAGGTTTTGGATGTAACTAAATTGGCATTATTGAGACCAGATGGTCATCCAGGTCCTTACATGAATCCTTTTCCATTTGCAAATGGAGTTCCAAAATATGTACAAAATGATTGTGTTCATTGGTGCTTGCCAGGACCTATAGATACTTGGAATGAGATTTTTGTAGAGATGATGAAGAAGTGGGAGTAA
Protein sequence

>MS.gene43909.t1

MGSTNPFNDNLSSFNKKFFHLTLYSLLPISLLCLYFIILSLTPFAPTKLLHSTTISTYHYSSTYSSPPSTSFVGKNKVYDYEKSCEYSNGDWVSDMRGPIYNVTTCGTIKESERCITNGRPDLGYLYWRWKPSECNLPRFEPNTFLKLVKNKHIAFVGDSLARNQLESLLCMLATTSTPNLVYQDGKSNKFRKWHFPSHNATFSLYWSPFLVHGVERSNEGNYYNTMFLDLVNEKWAKDIDQMDMIVISTGHWFLLPSIYYESDLVLGSLNCPELNHTQIDFYVPLTKALRTTLNSIIERKVAKGNGIDVIVKTFSPAHFEGDWDKAGTCSKTKPYRKEDKIVGGMDGEIRRIVMEEVESAKVKANKFEGIRLEVLDVTKLALLRPDGHPGPYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEIFVEMMKKWE