Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44192.t1 | GAU34245.1 | 84.7 | 157 | 24 | 0 | 1 | 157 | 2 | 158 | 7.60E-76 | 293.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44192.t1 | Q8GXB1 | 62.7 | 142 | 53 | 0 | 1 | 142 | 1 | 142 | 2.2e-52 | 206.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44192.t1 | A0A2Z6NEB4 | 84.7 | 157 | 24 | 0 | 1 | 157 | 2 | 158 | 5.5e-76 | 293.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052176 | MS.gene44192 | 0.816871 | 4.42E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene44192 | 0.810083 | 1.37E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene44192 | -0.802597 | 5.20E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44192.t1 | MTR_8g074720 | 95.070 | 142 | 7 | 0 | 1 | 142 | 1 | 142 | 1.22e-99 | 285 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44192.t1 | AT2G17695 | 62.676 | 142 | 53 | 0 | 1 | 142 | 1 | 142 | 1.74e-69 | 209 |
| MS.gene44192.t1 | AT2G17695 | 62.676 | 142 | 53 | 0 | 1 | 142 | 1 | 142 | 1.74e-69 | 209 |
| MS.gene44192.t1 | AT2G17695 | 62.676 | 142 | 53 | 0 | 1 | 142 | 10 | 151 | 1.93e-69 | 209 |
Find 34 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAACCCTTTATCGTCTTTA+AGG | 0.082122 | 8.1:-32134590 | None:intergenic |
| GTTTGGAAGTGGAACTCTTC+AGG | 0.224519 | 8.1:+32135865 | MS.gene44192:CDS |
| AAGATTCTTTGCAGGCATTT+TGG | 0.235249 | 8.1:+32135686 | MS.gene44192:intron |
| TCTTCCCTTAAAGACGATAA+AGG | 0.293197 | 8.1:+32134585 | MS.gene44192:CDS |
| CTGGCTTATGATGCCGCTTC+AGG | 0.295615 | 8.1:+32135790 | MS.gene44192:CDS |
| ACTCAATAACGCGCGAGTTC+TGG | 0.297727 | 8.1:+32134629 | MS.gene44192:CDS |
| CGTGGGGCCTCCTTTGGGTT+TGG | 0.321950 | 8.1:+32135848 | MS.gene44192:CDS |
| GCCTCCTTTGGGTTTGGAAG+TGG | 0.346292 | 8.1:+32135854 | MS.gene44192:CDS |
| GCGCGAGTTCTGGTGGGTTC+CGG | 0.368936 | 8.1:+32134639 | MS.gene44192:CDS |
| CTTCCCTTAAAGACGATAAA+GGG | 0.373019 | 8.1:+32134586 | MS.gene44192:CDS |
| TAACCCTTTATCGTCTTTAA+GGG | 0.383583 | 8.1:-32134589 | None:intergenic |
| AAAACCGTGGGGCCTCCTTT+GGG | 0.408508 | 8.1:+32135843 | MS.gene44192:CDS |
| GTGTCAAAGAGTTCCTTCCC+TGG | 0.464338 | 8.1:+32135771 | MS.gene44192:CDS |
| CCCTCACTGCATGTAGTTGT+TGG | 0.470968 | 8.1:-32136005 | None:intergenic |
| GAAAGAGTGCTCTCCGAAGT+TGG | 0.473870 | 8.1:+32134682 | MS.gene44192:CDS |
| GATAAAGGGTTATCGAAAGA+TGG | 0.480983 | 8.1:+32134600 | MS.gene44192:CDS |
| TTTGGAAGTGGAACTCTTCA+GGG | 0.481974 | 8.1:+32135866 | MS.gene44192:CDS |
| CAATAACGCGCGAGTTCTGG+TGG | 0.482031 | 8.1:+32134632 | MS.gene44192:CDS |
| CTGAAGCGGCATCATAAGCC+AGG | 0.497798 | 8.1:-32135789 | None:intergenic |
| AATAAACATACCTCCAACTT+CGG | 0.511559 | 8.1:-32134695 | None:intergenic |
| TTTACATACACAACCTGAAG+CGG | 0.542995 | 8.1:-32135803 | None:intergenic |
| CCGAAAACACCGGTTGAACA+AGG | 0.547031 | 8.1:+32135731 | MS.gene44192:CDS |
| CCAACAACTACATGCAGTGA+GGG | 0.559025 | 8.1:+32136005 | MS.gene44192:CDS |
| ATCGTCTTTAAGGGAAGACA+AGG | 0.586573 | 8.1:-32134580 | None:intergenic |
| AATTACGATGATAAATACAA+AGG | 0.625240 | 8.1:+32134543 | MS.gene44192:CDS |
| AGAGTGCTCTCCGAAGTTGG+AGG | 0.631829 | 8.1:+32134685 | MS.gene44192:CDS |
| ATTTGTTGATCCGAAAACAC+CGG | 0.633012 | 8.1:+32135721 | MS.gene44192:CDS |
| GCGGCATCATAAGCCAGGGA+AGG | 0.641751 | 8.1:-32135784 | None:intergenic |
| TCCACTTCCAAACCCAAAGG+AGG | 0.641963 | 8.1:-32135855 | None:intergenic |
| CAAACCCAAAGGAGGCCCCA+CGG | 0.643179 | 8.1:-32135847 | None:intergenic |
| TGAAGCGGCATCATAAGCCA+GGG | 0.652158 | 8.1:-32135788 | None:intergenic |
| GCCAACAACTACATGCAGTG+AGG | 0.671095 | 8.1:+32136004 | MS.gene44192:CDS |
| AGTTCCACTTCCAAACCCAA+AGG | 0.674733 | 8.1:-32135858 | None:intergenic |
| AATAACGCGCGAGTTCTGGT+GGG | 0.678093 | 8.1:+32134633 | MS.gene44192:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTAAAATTTAAGTTTGAT+TGG | + | chr8.1:32135565-32135584 | MS.gene44192:intron | 10.0% |
| !! | AATAAATGTGTATTCAGAAT+TGG | + | chr8.1:32135512-32135531 | MS.gene44192:intron | 20.0% |
| !! | AATTACGATGATAAATACAA+AGG | + | chr8.1:32134543-32134562 | MS.gene44192:CDS | 20.0% |
| !! | TAATGAGTAAATAGACAAAT+TGG | + | chr8.1:32134809-32134828 | MS.gene44192:intron | 20.0% |
| !! | TTAGTAATTGCAATTATGAA+TGG | + | chr8.1:32134865-32134884 | MS.gene44192:intron | 20.0% |
| ! | TAATACATACGCATTAATCA+AGG | - | chr8.1:32135645-32135664 | None:intergenic | 25.0% |
| ! | TGTACTTGATTGAATTGAAT+GGG | - | chr8.1:32135404-32135423 | None:intergenic | 25.0% |
| ! | TTGTACTTGATTGAATTGAA+TGG | - | chr8.1:32135405-32135424 | None:intergenic | 25.0% |
| !! | ACTTGTTTATGCAATCTTTT+TGG | - | chr8.1:32134417-32134436 | None:intergenic | 25.0% |
| !! | ATCATAAGCAAATTAGTCTA+TGG | + | chr8.1:32134932-32134951 | MS.gene44192:intron | 25.0% |
| !! | GTTTTCTAAGTGAGAAATAA+GGG | - | chr8.1:32135595-32135614 | None:intergenic | 25.0% |
| !! | TGTTTTCTAAGTGAGAAATA+AGG | - | chr8.1:32135596-32135615 | None:intergenic | 25.0% |
| AAGAAAATCAGTAAGATGTG+AGG | - | chr8.1:32135914-32135933 | None:intergenic | 30.0% | |
| AATAAACATACCTCCAACTT+CGG | - | chr8.1:32134698-32134717 | None:intergenic | 30.0% | |
| ACATGATGCTTGAAATTTCA+AGG | - | chr8.1:32134836-32134855 | None:intergenic | 30.0% | |
| AGGCATGTTAACAAAATATG+TGG | - | chr8.1:32135148-32135167 | None:intergenic | 30.0% | |
| ATAACCCTTTATCGTCTTTA+AGG | - | chr8.1:32134593-32134612 | None:intergenic | 30.0% | |
| CACATGATTCATTCAACATT+TGG | + | chr8.1:32134450-32134469 | MS.gene44192:intron | 30.0% | |
| GCACTAACAAACAAGTAAAT+TGG | - | chr8.1:32135300-32135319 | None:intergenic | 30.0% | |
| GCTTCTCAATACTTCTATTA+AGG | + | chr8.1:32135465-32135484 | MS.gene44192:intron | 30.0% | |
| GTGTCTGATTGAAAATATGA+AGG | + | chr8.1:32135953-32135972 | MS.gene44192:intron | 30.0% | |
| TAACCCTTTATCGTCTTTAA+GGG | - | chr8.1:32134592-32134611 | None:intergenic | 30.0% | |
| TGCATATCAGTTTGAACTTT+AGG | + | chr8.1:32134724-32134743 | MS.gene44192:intron | 30.0% | |
| TGGTATTAACCTATGAAACA+CGG | + | chr8.1:32134960-32134979 | MS.gene44192:intron | 30.0% | |
| TTTATGTGAAGATTCTTTGC+AGG | + | chr8.1:32135678-32135697 | MS.gene44192:intron | 30.0% | |
| ! | TCGAAGTTCATGATTTTCAA+TGG | + | chr8.1:32134482-32134501 | MS.gene44192:intron | 30.0% |
| ! | TGATTCTTTATTTACCAGGT+CGG | + | chr8.1:32134512-32134531 | MS.gene44192:intron | 30.0% |
| ! | TGTCTGATTCTTTATTTACC+AGG | + | chr8.1:32134508-32134527 | MS.gene44192:intron | 30.0% |
| !! | AATAGAAGTATTGAGAAGCA+TGG | - | chr8.1:32135464-32135483 | None:intergenic | 30.0% |
| !! | AGCTCATGATTTTTGAGAAT+TGG | - | chr8.1:32135248-32135267 | None:intergenic | 30.0% |
| !! | TATGCAATCTTTTTGGTCTT+GGG | - | chr8.1:32134410-32134429 | None:intergenic | 30.0% |
| !! | TTATGCAATCTTTTTGGTCT+TGG | - | chr8.1:32134411-32134430 | None:intergenic | 30.0% |
| CTATTAAGGACAAACCTTTG+AGG | + | chr8.1:32135479-32135498 | MS.gene44192:intron | 35.0% | |
| CTTCCCTTAAAGACGATAAA+GGG | + | chr8.1:32134586-32134605 | MS.gene44192:CDS | 35.0% | |
| CTTCTGATAAAAGACATGTC+CGG | - | chr8.1:32135087-32135106 | None:intergenic | 35.0% | |
| GATAAAGGGTTATCGAAAGA+TGG | + | chr8.1:32134600-32134619 | MS.gene44192:CDS | 35.0% | |
| TCTTCCCTTAAAGACGATAA+AGG | + | chr8.1:32134585-32134604 | MS.gene44192:CDS | 35.0% | |
| TGCTTATGATTATGACGCTT+AGG | - | chr8.1:32134922-32134941 | None:intergenic | 35.0% | |
| TTTACATACACAACCTGAAG+CGG | - | chr8.1:32135806-32135825 | None:intergenic | 35.0% | |
| ! | AAGATTCTTTGCAGGCATTT+TGG | + | chr8.1:32135686-32135705 | MS.gene44192:intron | 35.0% |
| ! | AATTTTGCTCCTTGTTCAAC+CGG | - | chr8.1:32135743-32135762 | None:intergenic | 35.0% |
| ! | ATTTGTTGATCCGAAAACAC+CGG | + | chr8.1:32135721-32135740 | MS.gene44192:CDS | 35.0% |
| ! | CAAATTAGTCTATGGTGTTG+TGG | + | chr8.1:32134940-32134959 | MS.gene44192:intron | 35.0% |
| ! | CTTTTCAAAAGTCTCAACAC+CGG | - | chr8.1:32134661-32134680 | None:intergenic | 35.0% |
| ! | GATTCTTTATTTACCAGGTC+GGG | + | chr8.1:32134513-32134532 | MS.gene44192:intron | 35.0% |
| !! | GGTGTTGAGACTTTTGAAAA+GGG | + | chr8.1:32134660-32134679 | MS.gene44192:CDS | 35.0% |
| !!! | CAGGCATTTTGGAATGAATT+GGG | + | chr8.1:32135697-32135716 | MS.gene44192:intron | 35.0% |
| !!! | TTTTATCAGAAGTGTCACAG+AGG | + | chr8.1:32135095-32135114 | MS.gene44192:intron | 35.0% |
| ACCGTTAGACACTATTGACA+AGG | - | chr8.1:32135168-32135187 | None:intergenic | 40.0% | |
| GCCTTGTCAATAGTGTCTAA+CGG | + | chr8.1:32135164-32135183 | MS.gene44192:intron | 40.0% | |
| GTCATCGAAACTGTAAGTGT+AGG | - | chr8.1:32135278-32135297 | None:intergenic | 40.0% | |
| TATGACGCTTAGGTCTTTCA+AGG | - | chr8.1:32134912-32134931 | None:intergenic | 40.0% | |
| TATTTGCGCTATAGCCTCAA+AGG | - | chr8.1:32135496-32135515 | None:intergenic | 40.0% | |
| TTTGGAAGTGGAACTCTTCA+GGG | + | chr8.1:32135866-32135885 | MS.gene44192:CDS | 40.0% | |
| ! | ATCGTCTTTAAGGGAAGACA+AGG | - | chr8.1:32134583-32134602 | None:intergenic | 40.0% |
| ! | GTATTGACATGGGATCAACA+TGG | + | chr8.1:32135117-32135136 | MS.gene44192:intron | 40.0% |
| !! | CGGTGTTGAGACTTTTGAAA+AGG | + | chr8.1:32134659-32134678 | MS.gene44192:CDS | 40.0% |
| !! | TGTCACAGAGGTATTGACAT+GGG | + | chr8.1:32135107-32135126 | MS.gene44192:intron | 40.0% |
| !!! | ATCTTTTTGGTCTTGGGTAG+TGG | - | chr8.1:32134404-32134423 | None:intergenic | 40.0% |
| !!! | GCAGGCATTTTGGAATGAAT+TGG | + | chr8.1:32135696-32135715 | MS.gene44192:intron | 40.0% |
| !!! | TCTTTTTGGTCTTGGGTAGT+GGG | - | chr8.1:32134403-32134422 | None:intergenic | 40.0% |
| AACTCTTCAGGGTCACTTAC+TGG | + | chr8.1:32135877-32135896 | MS.gene44192:intron | 45.0% | |
| ACTAGCACTACCAAAAACCG+TGG | + | chr8.1:32135830-32135849 | MS.gene44192:CDS | 45.0% | |
| AGTTCCACTTCCAAACCCAA+AGG | - | chr8.1:32135861-32135880 | None:intergenic | 45.0% | |
| CTAGCACTACCAAAAACCGT+GGG | + | chr8.1:32135831-32135850 | MS.gene44192:CDS | 45.0% | |
| GTTTGGAAGTGGAACTCTTC+AGG | + | chr8.1:32135865-32135884 | MS.gene44192:CDS | 45.0% | |
| TAGCACTACCAAAAACCGTG+GGG | + | chr8.1:32135832-32135851 | MS.gene44192:CDS | 45.0% | |
| TGTCTGTGTCCGTGTTTCAT+AGG | - | chr8.1:32134972-32134991 | None:intergenic | 45.0% | |
| ! | CCTTGTTCAACCGGTGTTTT+CGG | - | chr8.1:32135734-32135753 | None:intergenic | 45.0% |
| ! | GTAATTCAAAGTACCCGACC+TGG | - | chr8.1:32134529-32134548 | None:intergenic | 45.0% |
| !! | GTGTCACAGAGGTATTGACA+TGG | + | chr8.1:32135106-32135125 | MS.gene44192:intron | 45.0% |
| ACTCAATAACGCGCGAGTTC+TGG | + | chr8.1:32134629-32134648 | MS.gene44192:CDS | 50.0% | |
| CCATGACACATGTTGGACAC+CGG | + | chr8.1:32135065-32135084 | MS.gene44192:intron | 50.0% | |
| GATCAGAGAGAGGAGAGGAT+TGG | - | chr8.1:32135442-32135461 | None:intergenic | 50.0% | |
| GTGTCAAAGAGTTCCTTCCC+TGG | + | chr8.1:32135771-32135790 | MS.gene44192:CDS | 50.0% | |
| TCCACTTCCAAACCCAAAGG+AGG | - | chr8.1:32135858-32135877 | None:intergenic | 50.0% | |
| TGAAGCGGCATCATAAGCCA+GGG | - | chr8.1:32135791-32135810 | None:intergenic | 50.0% | |
| ! | AATAACGCGCGAGTTCTGGT+GGG | + | chr8.1:32134633-32134652 | MS.gene44192:CDS | 50.0% |
| ! | CCGAAAACACCGGTTGAACA+AGG | + | chr8.1:32135731-32135750 | MS.gene44192:CDS | 50.0% |
| ! | GAGAAGCATGGATCAGAGAG+AGG | - | chr8.1:32135452-32135471 | None:intergenic | 50.0% |
| ! | GTGTCGACCATGACACATGT+TGG | + | chr8.1:32135058-32135077 | MS.gene44192:intron | 50.0% |
| !! | GAAAGAGTGCTCTCCGAAGT+TGG | + | chr8.1:32134682-32134701 | MS.gene44192:CDS | 50.0% |
| AAAAACCGTGGGGCCTCCTT+TGG | + | chr8.1:32135842-32135861 | MS.gene44192:CDS | 55.0% | |
| AAAACCGTGGGGCCTCCTTT+GGG | + | chr8.1:32135843-32135862 | MS.gene44192:CDS | 55.0% | |
| CCGGTGTCCAACATGTGTCA+TGG | - | chr8.1:32135068-32135087 | None:intergenic | 55.0% | |
| CTGAAGCGGCATCATAAGCC+AGG | - | chr8.1:32135792-32135811 | None:intergenic | 55.0% | |
| GCATGGATCAGAGAGAGGAG+AGG | - | chr8.1:32135447-32135466 | None:intergenic | 55.0% | |
| ! | AAAGGAGGCCCCACGGTTTT+TGG | - | chr8.1:32135843-32135862 | None:intergenic | 55.0% |
| ! | AGAGTGCTCTCCGAAGTTGG+AGG | + | chr8.1:32134685-32134704 | MS.gene44192:CDS | 55.0% |
| ! | CAATAACGCGCGAGTTCTGG+TGG | + | chr8.1:32134632-32134651 | MS.gene44192:CDS | 55.0% |
| ! | CTGGCTTATGATGCCGCTTC+AGG | + | chr8.1:32135790-32135809 | MS.gene44192:CDS | 55.0% |
| ! | GCCTCCTTTGGGTTTGGAAG+TGG | + | chr8.1:32135854-32135873 | MS.gene44192:CDS | 55.0% |
| CAAACCCAAAGGAGGCCCCA+CGG | - | chr8.1:32135850-32135869 | None:intergenic | 60.0% | |
| GCGGCATCATAAGCCAGGGA+AGG | - | chr8.1:32135787-32135806 | None:intergenic | 60.0% | |
| ! | CGTGGGGCCTCCTTTGGGTT+TGG | + | chr8.1:32135848-32135867 | MS.gene44192:CDS | 65.0% |
| !! | GCGCGAGTTCTGGTGGGTTC+CGG | + | chr8.1:32134639-32134658 | MS.gene44192:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 32134376 | 32136024 | 32134376 | ID=MS.gene44192 |
| chr8.1 | mRNA | 32134376 | 32136024 | 32134376 | ID=MS.gene44192.t1;Parent=MS.gene44192 |
| chr8.1 | exon | 32134376 | 32134434 | 32134376 | ID=MS.gene44192.t1.exon1;Parent=MS.gene44192.t1 |
| chr8.1 | CDS | 32134376 | 32134434 | 32134376 | ID=cds.MS.gene44192.t1;Parent=MS.gene44192.t1 |
| chr8.1 | exon | 32134530 | 32134706 | 32134530 | ID=MS.gene44192.t1.exon2;Parent=MS.gene44192.t1 |
| chr8.1 | CDS | 32134530 | 32134706 | 32134530 | ID=cds.MS.gene44192.t1;Parent=MS.gene44192.t1 |
| chr8.1 | exon | 32135700 | 32135887 | 32135700 | ID=MS.gene44192.t1.exon3;Parent=MS.gene44192.t1 |
| chr8.1 | CDS | 32135700 | 32135887 | 32135700 | ID=cds.MS.gene44192.t1;Parent=MS.gene44192.t1 |
| chr8.1 | exon | 32135975 | 32136024 | 32135975 | ID=MS.gene44192.t1.exon4;Parent=MS.gene44192.t1 |
| chr8.1 | CDS | 32135975 | 32136024 | 32135975 | ID=cds.MS.gene44192.t1;Parent=MS.gene44192.t1 |
| Gene Sequence |
| Protein sequence |
