Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44391.t1 | XP_024625196.1 | 94.4 | 125 | 7 | 0 | 1 | 125 | 34 | 158 | 8.50E-62 | 246.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44391.t1 | G7KSL2 | 94.4 | 125 | 7 | 0 | 1 | 125 | 34 | 158 | 6.1e-62 | 246.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058783 | MS.gene44391 | 0.800516 | 1.39E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGATTCTGAACTTTATT+TGG | 0.166699 | 7.3:+18204703 | MS.gene44391:CDS |
| TGCATCCCAAAGTGGTAAAC+TGG | 0.283430 | 7.3:+18204549 | MS.gene44391:CDS |
| CTCTTGAATCAGTCCTCTTC+TGG | 0.284774 | 7.3:+18204803 | MS.gene44391:CDS |
| TCGTCCCAGTTTACCACTTT+GGG | 0.328360 | 7.3:-18204554 | None:intergenic |
| CGCCCCAACATAGAAGAAAA+AGG | 0.348181 | 7.3:+18204749 | MS.gene44391:CDS |
| ACATCATCTATGTAAATGTT+AGG | 0.394819 | 7.3:-18204665 | None:intergenic |
| GTCGTCCCAGTTTACCACTT+TGG | 0.436251 | 7.3:-18204555 | None:intergenic |
| GGTTTCCAACAGTGCCTTTG+TGG | 0.441064 | 7.3:+18204453 | MS.gene44391:CDS |
| CTTCGGTGGGCTCAGTTCCA+TGG | 0.462514 | 7.3:+18204492 | MS.gene44391:CDS |
| CTGAGATCAACAACATTCCC+TGG | 0.470295 | 7.3:+18204624 | MS.gene44391:CDS |
| TCTATGTAAATGTTAGGATC+AGG | 0.480083 | 7.3:-18204659 | None:intergenic |
| TTCTGAACTTTATTTGGATG+TGG | 0.480333 | 7.3:+18204709 | MS.gene44391:CDS |
| GTTTCCAACAGTGCCTTTGT+GGG | 0.503784 | 7.3:+18204454 | MS.gene44391:CDS |
| ATCAGGCAATGGTATGTCCC+AGG | 0.512479 | 7.3:-18204642 | None:intergenic |
| GGAGAGGCGGCTGTGACTCT+AGG | 0.522716 | 7.3:+18204770 | MS.gene44391:CDS |
| TCTTCTATTAGCTTTCGCCA+TGG | 0.571899 | 7.3:-18204509 | None:intergenic |
| TCCCAATCCAAGACCAGAAG+AGG | 0.573717 | 7.3:-18204816 | None:intergenic |
| GCATCCCAAAGTGGTAAACT+GGG | 0.611168 | 7.3:+18204550 | MS.gene44391:CDS |
| GAAAGCTAATAGAAGACAAG+AGG | 0.621891 | 7.3:+18204516 | MS.gene44391:CDS |
| GGACGACTCTGCTGTCAAAG+AGG | 0.623703 | 7.3:+18204571 | MS.gene44391:CDS |
| GGATGTGGAAATAGAAGCAG+AGG | 0.625331 | 7.3:+18204724 | MS.gene44391:CDS |
| TGAGATCAACAACATTCCCT+GGG | 0.626226 | 7.3:+18204625 | MS.gene44391:CDS |
| CAGAAGAGGACTGATTCAAG+AGG | 0.638374 | 7.3:-18204802 | None:intergenic |
| CATGTATATGCATCCCAAAG+TGG | 0.643906 | 7.3:+18204541 | MS.gene44391:CDS |
| TCAGGCAATGGTATGTCCCA+GGG | 0.651273 | 7.3:-18204641 | None:intergenic |
| TAAATGTTAGGATCAGGCAA+TGG | 0.686387 | 7.3:-18204653 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACATCATCTATGTAAATGTT+AGG | - | chr7.3:18204668-18204687 | None:intergenic | 25.0% |
| !!! | TGTTGATTCTGAACTTTATT+TGG | + | chr7.3:18204703-18204722 | MS.gene44391:CDS | 25.0% |
| ACAATGCAAAAAACAGGTAT+TGG | + | chr7.3:18204600-18204619 | MS.gene44391:CDS | 30.0% | |
| CAATGCAAAAAACAGGTATT+GGG | + | chr7.3:18204601-18204620 | MS.gene44391:CDS | 30.0% | |
| TCTATGTAAATGTTAGGATC+AGG | - | chr7.3:18204662-18204681 | None:intergenic | 30.0% | |
| ! | TCTCCTTTTTCTTCTATGTT+GGG | - | chr7.3:18204755-18204774 | None:intergenic | 30.0% |
| ! | TTCTGAACTTTATTTGGATG+TGG | + | chr7.3:18204709-18204728 | MS.gene44391:CDS | 30.0% |
| CAACATAGAAGAAAAAGGAG+AGG | + | chr7.3:18204754-18204773 | MS.gene44391:CDS | 35.0% | |
| CATTCGACAATGCAAAAAAC+AGG | + | chr7.3:18204594-18204613 | MS.gene44391:CDS | 35.0% | |
| GAAAGCTAATAGAAGACAAG+AGG | + | chr7.3:18204516-18204535 | MS.gene44391:CDS | 35.0% | |
| TAAATGTTAGGATCAGGCAA+TGG | - | chr7.3:18204656-18204675 | None:intergenic | 35.0% | |
| ! | CTCCTTTTTCTTCTATGTTG+GGG | - | chr7.3:18204754-18204773 | None:intergenic | 35.0% |
| ! | CTCTCCTTTTTCTTCTATGT+TGG | - | chr7.3:18204756-18204775 | None:intergenic | 35.0% |
| CATAGAAGAAAAAGGAGAGG+CGG | + | chr7.3:18204757-18204776 | MS.gene44391:CDS | 40.0% | |
| CATGTATATGCATCCCAAAG+TGG | + | chr7.3:18204541-18204560 | MS.gene44391:CDS | 40.0% | |
| TGAGATCAACAACATTCCCT+GGG | + | chr7.3:18204625-18204644 | MS.gene44391:CDS | 40.0% | |
| ! | CTTTTTCTTCTATGTTGGGG+CGG | - | chr7.3:18204751-18204770 | None:intergenic | 40.0% |
| ! | TTTTTCTTCTATGTTGGGGC+GGG | - | chr7.3:18204750-18204769 | None:intergenic | 40.0% |
| !! | CAAAACTGCTTTTCCCACAA+AGG | - | chr7.3:18204470-18204489 | None:intergenic | 40.0% |
| !! | GGAAAAGCAGTTTTGTGCTT+CGG | + | chr7.3:18204475-18204494 | MS.gene44391:CDS | 40.0% |
| !! | TCTTCTATTAGCTTTCGCCA+TGG | - | chr7.3:18204512-18204531 | None:intergenic | 40.0% |
| CGCCCCAACATAGAAGAAAA+AGG | + | chr7.3:18204749-18204768 | MS.gene44391:CDS | 45.0% | |
| CTGAGATCAACAACATTCCC+TGG | + | chr7.3:18204624-18204643 | MS.gene44391:CDS | 45.0% | |
| GCATCCCAAAGTGGTAAACT+GGG | + | chr7.3:18204550-18204569 | MS.gene44391:CDS | 45.0% | |
| GGATGTGGAAATAGAAGCAG+AGG | + | chr7.3:18204724-18204743 | MS.gene44391:CDS | 45.0% | |
| GTTTCCAACAGTGCCTTTGT+GGG | + | chr7.3:18204454-18204473 | MS.gene44391:CDS | 45.0% | |
| TCGTCCCAGTTTACCACTTT+GGG | - | chr7.3:18204557-18204576 | None:intergenic | 45.0% | |
| TGCATCCCAAAGTGGTAAAC+TGG | + | chr7.3:18204549-18204568 | MS.gene44391:CDS | 45.0% | |
| ! | CAGAAGAGGACTGATTCAAG+AGG | - | chr7.3:18204805-18204824 | None:intergenic | 45.0% |
| !! | AAAGCAGTTTTGTGCTTCGG+TGG | + | chr7.3:18204478-18204497 | MS.gene44391:CDS | 45.0% |
| !! | AAGCAGTTTTGTGCTTCGGT+GGG | + | chr7.3:18204479-18204498 | MS.gene44391:CDS | 45.0% |
| !!! | TTTTCCCACAAAGGCACTGT+TGG | - | chr7.3:18204461-18204480 | None:intergenic | 45.0% |
| ATCAGGCAATGGTATGTCCC+AGG | - | chr7.3:18204645-18204664 | None:intergenic | 50.0% | |
| GGTTTCCAACAGTGCCTTTG+TGG | + | chr7.3:18204453-18204472 | MS.gene44391:CDS | 50.0% | |
| GTCGTCCCAGTTTACCACTT+TGG | - | chr7.3:18204558-18204577 | None:intergenic | 50.0% | |
| TCAGGCAATGGTATGTCCCA+GGG | - | chr7.3:18204644-18204663 | None:intergenic | 50.0% | |
| GGACGACTCTGCTGTCAAAG+AGG | + | chr7.3:18204571-18204590 | MS.gene44391:CDS | 55.0% | |
| ! | CTTCGGTGGGCTCAGTTCCA+TGG | + | chr7.3:18204492-18204511 | MS.gene44391:CDS | 60.0% |
| GGAGAGGCGGCTGTGACTCT+AGG | + | chr7.3:18204770-18204789 | MS.gene44391:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 18204447 | 18204824 | 18204447 | ID=MS.gene44391 |
| chr7.3 | mRNA | 18204447 | 18204824 | 18204447 | ID=MS.gene44391.t1;Parent=MS.gene44391 |
| chr7.3 | exon | 18204447 | 18204824 | 18204447 | ID=MS.gene44391.t1.exon1;Parent=MS.gene44391.t1 |
| chr7.3 | CDS | 18204447 | 18204824 | 18204447 | ID=cds.MS.gene44391.t1;Parent=MS.gene44391.t1 |
| Gene Sequence |
| Protein sequence |
