AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44486

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44486.t1 MTR_4g095430 97.819 596 12 1 1 596 1 595 0.0 1199
MS.gene44486.t1 MTR_5g016060 70.492 610 159 10 1 596 28 630 0.0 857
MS.gene44486.t1 MTR_5g016060 69.866 521 138 9 90 596 1 516 0.0 715
MS.gene44486.t1 MTR_4g022330 41.065 582 311 10 2 571 24 585 5.81e-152 451
MS.gene44486.t1 MTR_7g108290 41.107 596 298 17 1 569 25 594 4.31e-137 414
MS.gene44486.t1 MTR_1g088885 39.103 624 325 16 1 596 25 621 5.55e-137 412
MS.gene44486.t1 MTR_7g073980 41.289 574 299 13 12 571 32 581 6.69e-137 413
MS.gene44486.t1 MTR_3g062540 40.000 575 291 10 1 568 12 539 5.41e-135 405
MS.gene44486.t1 MTR_5g075640 37.500 616 316 11 1 596 13 579 1.74e-134 405
MS.gene44486.t1 MTR_4g093850 36.473 584 311 9 2 527 33 614 2.62e-119 369
MS.gene44486.t1 MTR_3g062540 39.227 543 276 10 33 568 2 497 2.26e-118 361
MS.gene44486.t1 MTR_2g009340 39.015 528 250 8 1 466 32 549 8.99e-117 362
MS.gene44486.t1 MTR_5g075640 35.788 584 306 11 33 596 2 536 1.34e-115 355
MS.gene44486.t1 MTR_1g105870 81.364 220 18 2 20 239 38 234 3.45e-107 323
MS.gene44486.t1 MTR_8g078900 38.416 505 265 11 7 467 14 516 3.09e-105 332
MS.gene44486.t1 MTR_2g018750 35.976 492 283 9 7 472 20 505 9.88e-104 326
MS.gene44486.t1 MTR_1g094850 36.495 485 286 8 7 472 20 501 8.29e-102 321
MS.gene44486.t1 MTR_8g090220 36.420 486 240 8 13 460 52 506 3.20e-101 317
MS.gene44486.t1 MTR_8g090220 35.897 507 245 9 13 470 52 527 4.57e-101 320
MS.gene44486.t1 MTR_8g078900 35.450 567 299 13 25 527 2 565 2.77e-100 318
MS.gene44486.t1 MTR_2g084595 33.759 548 336 10 6 527 16 562 4.30e-100 317
MS.gene44486.t1 MTR_4g093850 37.983 466 240 6 2 420 33 496 2.01e-98 310
MS.gene44486.t1 MTR_5g015790 37.100 469 273 7 10 465 24 483 3.01e-97 310
MS.gene44486.t1 MTR_8g078900 40.046 437 234 9 57 467 42 476 1.37e-94 303
MS.gene44486.t1 MTR_1g106430 36.253 491 282 9 10 485 25 499 1.67e-93 300
MS.gene44486.t1 MTR_1g014070 37.155 471 271 11 10 463 24 486 2.78e-93 299
MS.gene44486.t1 MTR_1g064360 34.151 489 273 8 7 470 20 484 7.97e-93 296
MS.gene44486.t1 MTR_5g032780 36.436 505 264 9 10 465 23 519 1.80e-92 298
MS.gene44486.t1 MTR_4g134210 36.147 462 280 6 10 465 23 475 3.41e-90 291
MS.gene44486.t1 MTR_2g048390 35.520 442 252 9 2 414 15 452 4.56e-87 278
MS.gene44486.t1 MTR_8g078900 36.283 452 242 11 7 414 14 463 4.79e-81 263
MS.gene44486.t1 MTR_8g069330 34.227 485 255 9 16 465 29 484 9.78e-79 260
MS.gene44486.t1 MTR_2g027410 34.279 458 280 9 12 465 25 465 6.98e-78 257
MS.gene44486.t1 MTR_1g082690 29.468 526 322 9 2 518 47 532 3.15e-77 255
MS.gene44486.t1 MTR_2g084595 33.721 430 264 7 6 414 16 445 1.49e-73 242
MS.gene44486.t1 MTR_8g078900 37.760 384 211 9 57 414 42 423 1.27e-70 234
MS.gene44486.t1 MTR_2g027410 33.333 408 253 7 62 465 1 393 1.12e-64 220
MS.gene44486.t1 MTR_4g011530 37.870 338 181 8 195 519 46 367 4.01e-55 193
MS.gene44486.t1 MTR_2g102730 36.723 354 193 9 195 536 24 358 1.08e-54 191
MS.gene44486.t1 MTR_4g045767 27.307 531 331 16 22 527 14 514 4.56e-46 171
MS.gene44486.t1 MTR_4g074190 25.046 539 351 13 16 527 6 518 6.65e-39 150
MS.gene44486.t1 MTR_4g074190 25.249 503 311 12 57 527 3 472 6.11e-38 147
MS.gene44486.t1 MTR_1g035720 24.819 552 329 20 16 527 6 511 3.80e-33 134
MS.gene44486.t1 MTR_4g074190 24.887 442 270 14 15 418 5 422 1.77e-28 119
MS.gene44486.t1 MTR_2g010890 25.871 402 253 11 82 467 2 374 4.23e-25 108
MS.gene44486.t1 MTR_2g048375 28.750 160 105 3 431 584 1 157 3.89e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44486.t1 AT5G67385 62.768 607 200 10 1 596 13 604 0.0 714
MS.gene44486.t1 AT5G67385 62.195 574 191 10 34 596 1 559 0.0 659
MS.gene44486.t1 AT5G67385 62.195 574 191 10 34 596 1 559 0.0 659
MS.gene44486.t1 AT5G67385 62.195 574 191 10 34 596 1 559 0.0 659
MS.gene44486.t1 AT5G67385 62.195 574 191 10 34 596 1 559 0.0 659
MS.gene44486.t1 AT3G49970 51.820 577 206 15 25 596 6 515 0.0 531
MS.gene44486.t1 AT3G49970 51.820 577 206 15 25 596 6 515 0.0 531
MS.gene44486.t1 AT5G48800 42.586 580 289 9 5 571 34 582 1.20e-153 455
MS.gene44486.t1 AT2G30520 38.599 614 321 10 1 596 18 593 1.04e-136 411
MS.gene44486.t1 AT2G47860 41.486 552 282 12 1 527 26 561 4.04e-131 398
MS.gene44486.t1 AT1G03010 38.898 635 324 18 1 596 25 634 3.37e-130 395
MS.gene44486.t1 AT1G03010 38.730 630 323 17 6 596 2 607 5.14e-129 392
MS.gene44486.t1 AT1G03010 38.942 624 318 17 12 596 34 633 1.69e-128 391
MS.gene44486.t1 AT3G08570 37.733 591 298 12 12 571 33 584 3.89e-127 387
MS.gene44486.t1 AT2G47860 39.826 575 282 13 1 527 26 584 6.25e-126 385
MS.gene44486.t1 AT2G30520 37.801 582 306 10 33 596 2 545 7.77e-120 366
MS.gene44486.t1 AT2G30520 37.801 582 306 10 33 596 2 545 7.77e-120 366
MS.gene44486.t1 AT3G08660 37.083 569 305 12 12 571 33 557 1.82e-115 356
MS.gene44486.t1 AT1G30440 35.516 611 337 15 2 566 15 614 3.11e-112 350
MS.gene44486.t1 AT5G03250 36.447 546 307 11 7 518 20 559 2.23e-107 335
MS.gene44486.t1 AT3G08660 37.153 541 287 12 40 571 1 497 7.39e-106 329
MS.gene44486.t1 AT2G47860 42.725 433 214 9 1 414 26 443 1.20e-103 321
MS.gene44486.t1 AT5G66560 33.851 644 331 18 7 566 13 645 2.06e-101 322
MS.gene44486.t1 AT5G13600 37.475 491 272 11 7 472 20 500 1.35e-100 317
MS.gene44486.t1 AT2G47860 40.570 456 214 10 1 414 26 466 1.65e-98 308
MS.gene44486.t1 AT5G66560 34.026 626 318 18 25 566 2 616 2.78e-96 308
MS.gene44486.t1 AT5G66560 35.740 554 303 16 55 566 64 606 6.23e-94 301
MS.gene44486.t1 AT2G47860 38.852 453 242 10 100 527 1 443 1.12e-90 289
MS.gene44486.t1 AT2G47860 38.852 453 242 10 100 527 1 443 1.12e-90 289
MS.gene44486.t1 AT2G14820 35.250 539 306 14 12 521 26 550 9.22e-89 288
MS.gene44486.t1 AT2G14820 35.250 539 306 14 12 521 26 550 9.22e-89 288
MS.gene44486.t1 AT4G31820 33.922 510 294 11 12 506 26 507 1.01e-87 283
MS.gene44486.t1 AT5G67440 35.729 487 286 11 10 483 24 496 3.26e-86 280
MS.gene44486.t1 AT5G67440 35.729 487 286 11 10 483 24 496 3.26e-86 280
MS.gene44486.t1 AT1G67900 37.349 498 259 9 10 465 23 509 1.36e-85 279
MS.gene44486.t1 AT1G67900 37.349 498 259 9 10 465 23 509 1.36e-85 279
MS.gene44486.t1 AT1G67900 37.349 498 259 9 10 465 23 509 1.36e-85 279
MS.gene44486.t1 AT1G67900 37.349 498 259 9 10 465 23 509 1.36e-85 279
MS.gene44486.t1 AT1G67900 37.349 498 259 9 10 465 23 509 1.36e-85 279
MS.gene44486.t1 AT1G67900 37.349 498 259 9 10 465 23 509 1.36e-85 279
MS.gene44486.t1 AT3G44820 29.982 567 350 11 2 527 12 572 1.13e-84 278
MS.gene44486.t1 AT3G50840 32.083 533 328 7 2 527 5 510 1.21e-84 275
MS.gene44486.t1 AT5G10250 33.932 501 265 10 2 467 39 508 4.09e-84 275
MS.gene44486.t1 AT4G37590 34.109 516 283 12 10 510 24 497 3.12e-83 272
MS.gene44486.t1 AT5G10250 34.151 489 265 10 2 467 39 493 4.88e-82 269
MS.gene44486.t1 AT5G67440 35.957 470 274 11 27 483 4 459 2.15e-80 263
MS.gene44486.t1 AT3G08660 36.804 413 225 8 12 416 33 417 4.36e-79 256
MS.gene44486.t1 AT2G23050 32.836 469 276 9 10 474 24 457 1.90e-75 248
MS.gene44486.t1 AT4G31820 32.780 482 281 11 40 506 1 454 3.13e-75 249
MS.gene44486.t1 AT3G26490 32.193 497 284 10 10 465 23 507 3.29e-75 251
MS.gene44486.t1 AT3G26490 32.193 497 284 10 10 465 23 507 3.29e-75 251
MS.gene44486.t1 AT3G26490 32.193 497 284 10 10 465 23 507 3.29e-75 251
MS.gene44486.t1 AT3G50840 31.737 501 308 7 34 527 1 474 1.59e-73 245
MS.gene44486.t1 AT3G50840 31.737 501 308 7 34 527 1 474 1.59e-73 245
MS.gene44486.t1 AT5G66560 33.970 471 244 11 7 414 13 479 2.60e-73 243
MS.gene44486.t1 AT5G03250 34.658 453 256 11 100 518 1 447 5.19e-73 242
MS.gene44486.t1 AT5G47800 30.801 487 295 12 12 482 25 485 4.08e-71 239
MS.gene44486.t1 AT5G47800 30.801 487 295 12 12 482 25 485 4.08e-71 239
MS.gene44486.t1 AT5G47800 30.801 487 295 12 12 482 25 485 4.08e-71 239
MS.gene44486.t1 AT5G47800 30.801 487 295 12 12 482 138 598 6.91e-70 238
MS.gene44486.t1 AT3G26490 30.872 447 256 10 10 415 23 457 7.44e-59 205
MS.gene44486.t1 AT5G48130 27.485 513 305 13 7 493 33 504 2.31e-53 192
MS.gene44486.t1 AT5G64330 39.922 258 131 4 3 239 42 296 4.56e-52 189
MS.gene44486.t1 AT5G64330 37.367 281 141 5 256 503 372 650 5.98e-49 181
MS.gene44486.t1 AT5G64330 39.922 258 131 4 3 239 42 296 1.76e-51 189
MS.gene44486.t1 AT5G64330 35.197 304 157 5 256 519 372 675 3.15e-49 182
MS.gene44486.t1 AT1G52770 33.333 357 200 7 195 522 28 375 4.07e-51 182
MS.gene44486.t1 AT5G64330 37.367 281 141 5 256 503 313 591 1.84e-49 181
MS.gene44486.t1 AT5G64330 39.827 231 115 4 30 239 10 237 3.92e-43 164
MS.gene44486.t1 AT2G23050 30.319 376 226 8 100 474 1 341 2.37e-48 173
MS.gene44486.t1 AT3G15570 34.965 286 159 5 204 471 38 314 4.77e-45 166
MS.gene44486.t1 AT3G19850 27.234 470 274 11 33 463 23 463 3.89e-42 160
MS.gene44486.t1 AT1G50280 28.602 465 291 11 15 453 5 454 3.40e-40 154
MS.gene44486.t1 AT3G22104 27.938 451 278 13 15 452 6 422 5.86e-39 150
MS.gene44486.t1 AT1G50280 29.356 419 259 9 57 453 18 421 2.50e-38 148
MS.gene44486.t1 AT5G17580 26.709 468 304 11 15 470 22 462 2.66e-37 146
MS.gene44486.t1 AT5G17580 25.977 512 336 12 15 510 7 491 3.67e-37 145
MS.gene44486.t1 AT3G03510 27.696 408 240 10 69 470 8 366 1.57e-34 136
MS.gene44486.t1 AT3G22104 26.862 376 233 9 87 452 4 347 7.05e-27 114
MS.gene44486.t1 AT3G22104 26.862 376 233 9 87 452 4 347 7.05e-27 114
MS.gene44486.t1 AT3G22104 26.862 376 233 9 87 452 4 347 7.05e-27 114
MS.gene44486.t1 AT3G49900 30.864 162 100 4 3 159 55 209 1.02e-13 74.3
MS.gene44486.t1 AT3G49900 30.864 162 100 4 3 159 55 209 1.13e-13 74.3

Find 103 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAATTTGAGCCACATTT+TGG 0.185225 8.1:+25820333 None:intergenic
GTCTAGTTGTATGCGATCTT+TGG 0.210752 8.1:+25819947 None:intergenic
CTTCCTTATGTGTCCACTTT+TGG 0.213031 8.1:+25822748 None:intergenic
AGATTATTCGGTTGGAAATT+TGG 0.233660 8.1:-25822546 MS.gene44486:CDS
CCATTCACTTGTTCTCTTCA+TGG 0.260781 8.1:+25823261 None:intergenic
TTCTGTCATTTCAAGATATT+CGG 0.262735 8.1:+25822568 None:intergenic
CTGTCAGCTTTCTTTCACTA+TGG 0.279955 8.1:-25821354 MS.gene44486:CDS
TTTAACAAAGGACTATTAAT+AGG 0.297500 8.1:+25820089 None:intergenic
TTGAGACAACTGTTGCTTGC+AGG 0.327110 8.1:-25821312 MS.gene44486:CDS
TTTGTGGAGCAGGAGATATT+TGG 0.340760 8.1:-25821475 MS.gene44486:intron
GCGCCTCTCGCGATAGCTTC+TGG 0.348002 8.1:+25820390 None:intergenic
TGAAATGACAGAAGATTATT+CGG 0.348779 8.1:-25822558 MS.gene44486:CDS
AGTGATGTCGCTATTCAAAT+TGG 0.360026 8.1:-25823101 MS.gene44486:CDS
TGTTGCTTGCAGGTTTGATT+TGG 0.361308 8.1:-25821302 MS.gene44486:CDS
CATTCTTCTCCCTTGGCATA+AGG 0.363104 8.1:+25821383 None:intergenic
CTTTCTTTCACTATGGCTTA+AGG 0.386758 8.1:-25821347 MS.gene44486:CDS
TCTTGCACAAAATTAGGATA+TGG 0.387255 8.1:+25819908 None:intergenic
AGAAGGATAGCCATGCAATT+AGG 0.395560 8.1:-25821277 MS.gene44486:CDS
TCTAGTTGTATGCGATCTTT+GGG 0.404869 8.1:+25819948 None:intergenic
AAGAGTTCTAATTGCAATGA+TGG 0.405470 8.1:-25822228 MS.gene44486:CDS
TGTGAATAAAGCATTATGAT+AGG 0.408553 8.1:+25822176 None:intergenic
TTTCCATTAATTTCCAAAAG+TGG 0.409367 8.1:-25822761 MS.gene44486:CDS
GCTTCCAAGGAGAGTCAGTT+TGG 0.413724 8.1:-25822377 MS.gene44486:CDS
TACCTTAAGATAGATGTCTA+TGG 0.417265 8.1:+25820958 None:intergenic
GTTGCACTAAAGAGCATGTC+AGG 0.420005 8.1:-25822494 MS.gene44486:CDS
GAGAGTCAGTTTGGTTCATC+TGG 0.422018 8.1:-25822368 MS.gene44486:CDS
ACATCGATGAGTTCAATTAA+TGG 0.432599 8.1:+25822689 None:intergenic
ACAGAAATCTCTGAGAGGTT+TGG 0.436903 8.1:-25822156 MS.gene44486:intron
TTTCCAAAAGTGGACACATA+AGG 0.437270 8.1:-25822751 MS.gene44486:CDS
ACCAATCAACAGCTGGCCTC+TGG 0.440922 8.1:+25822291 None:intergenic
CGACATCACTTGGAATCTCC+TGG 0.445103 8.1:+25823114 None:intergenic
TTCCTCTTGTCAGGTGTTGA+AGG 0.446976 8.1:+25820248 None:intergenic
TCAGCAAGACAGATAAACTT+TGG 0.448229 8.1:+25821028 None:intergenic
ACCAGAGGCCAGCTGTTGAT+TGG 0.449238 8.1:-25822292 MS.gene44486:CDS
CTGTCTGTGTCGCTTACAAA+TGG 0.449775 8.1:+25820440 None:intergenic
GAGTATACATTCCTCTTGTC+AGG 0.452843 8.1:+25820239 None:intergenic
AGCCATAGACATCTATCTTA+AGG 0.453245 8.1:-25820960 MS.gene44486:intron
AACTCAGTTTCCAGAAAACT+TGG 0.456593 8.1:-25820014 MS.gene44486:CDS
TAGCAGCAAAGTTCTGTTAC+GGG 0.457361 8.1:-25822640 MS.gene44486:CDS
AACTACCTTGTGTAACGAAT+AGG 0.461457 8.1:+25823070 None:intergenic
GATATTGCATTCTTCTCCCT+TGG 0.461512 8.1:+25821376 None:intergenic
AGAGGCCAGCTGTTGATTGG+TGG 0.462714 8.1:-25822289 MS.gene44486:CDS
CACACGCTGCACAAAACAAC+AGG 0.463975 8.1:-25820364 MS.gene44486:CDS
ATGACAGAAGATTATTCGGT+TGG 0.476245 8.1:-25822554 MS.gene44486:CDS
ATATTATTCTTCTCCACAAA+GGG 0.481505 8.1:-25821122 MS.gene44486:CDS
AAATCAAGAACAACAGAAGA+TGG 0.483167 8.1:-25820992 MS.gene44486:CDS
AAAGAAAGTTTGCAGCATGA+TGG 0.486600 8.1:-25820417 MS.gene44486:CDS
ACTCTCCTTGGAAGCTATGT+AGG 0.486973 8.1:+25822385 None:intergenic
TGAACCAAACTGACTCTCCT+TGG 0.490716 8.1:+25822373 None:intergenic
ACAGGATGAGCACGTTGAAA+AGG 0.498236 8.1:+25819981 None:intergenic
GATGTGTCGCCGCTAAATGA+AGG 0.498482 8.1:+25821226 None:intergenic
ATTTGAATAGCGACATCACT+TGG 0.501604 8.1:+25823104 None:intergenic
TTGGAAACCTCATTAGAAGC+TGG 0.504132 8.1:+25820203 None:intergenic
CTAGCAGCAAAGTTCTGTTA+CGG 0.504241 8.1:-25822641 MS.gene44486:CDS
TATTCGGTTGGAAATTTGGT+AGG 0.509761 8.1:-25822542 MS.gene44486:CDS
CTCTTCTCATGCTCTTCATG+TGG 0.516105 8.1:+25821430 None:intergenic
GATATTTGGAAAGGGAAGGA+AGG 0.520414 8.1:-25821461 MS.gene44486:CDS
AGTTTCTGATGTATGTAAGA+TGG 0.522762 8.1:+25822463 None:intergenic
GCTTTATCCAGCTTCTAATG+AGG 0.525232 8.1:-25820210 MS.gene44486:CDS
GAGCAGGAGATATTTGGAAA+GGG 0.525905 8.1:-25821469 MS.gene44486:intron
CGCGACAACATGGATGGTAC+TGG 0.526984 8.1:-25820290 MS.gene44486:CDS
GAGGCCAGCTGTTGATTGGT+GGG 0.529140 8.1:-25822288 MS.gene44486:CDS
CATCGCTATGCTTCGATGTG+TGG 0.533993 8.1:-25822591 MS.gene44486:CDS
GGTTCATCTGGTAGAAGTGA+TGG 0.534039 8.1:-25822356 MS.gene44486:CDS
ATGGATGGTACTGGCAGCAT+AGG 0.534255 8.1:-25820281 MS.gene44486:CDS
TCAGCCCACCAATCAACAGC+TGG 0.545487 8.1:+25822284 None:intergenic
CGCCTTCAACACCTGACAAG+AGG 0.552605 8.1:-25820250 MS.gene44486:CDS
TTATCACAGGAATTACTCCC+AGG 0.555974 8.1:-25823132 MS.gene44486:intron
GATAGCCATGCAATTAGGAC+AGG 0.556430 8.1:-25821272 MS.gene44486:CDS
AGGAGATATTTGGAAAGGGA+AGG 0.557338 8.1:-25821465 MS.gene44486:CDS
ATTGAACTCATCGATGTCCC+TGG 0.557723 8.1:-25822683 MS.gene44486:CDS
AATAGCCTACATAGCTTCCA+AGG 0.561754 8.1:-25822390 MS.gene44486:CDS
CCATGAAGAGAACAAGTGAA+TGG 0.562517 8.1:-25823261 MS.gene44486:intron
GCAATTAGGACAGGCTGTGT+TGG 0.562742 8.1:-25821263 MS.gene44486:CDS
GCAGGTTTGATTTGGAGAGA+AGG 0.563785 8.1:-25821294 MS.gene44486:CDS
TACTAGTGAGCCTTATGCCA+AGG 0.566316 8.1:-25821393 MS.gene44486:CDS
CACAGCCTGTCCTAATTGCA+TGG 0.571835 8.1:+25821267 None:intergenic
CGTCTTCGCGACAACATGGA+TGG 0.575028 8.1:-25820296 MS.gene44486:CDS
CTAATTGCAATGATGGCTAG+AGG 0.579105 8.1:-25822221 MS.gene44486:CDS
AATATTATTCTTCTCCACAA+AGG 0.580458 8.1:-25821123 MS.gene44486:CDS
GGAGCAGGAGATATTTGGAA+AGG 0.582870 8.1:-25821470 MS.gene44486:intron
GACATCACTTGGAATCTCCT+GGG 0.585133 8.1:+25823115 None:intergenic
TGGCCTCTGGTGAGAAGCAG+TGG 0.585433 8.1:+25822304 None:intergenic
ATTAGAAGCTGGATAAAGCT+CGG 0.591749 8.1:+25820214 None:intergenic
TCAAATGCTTCTGATCCACC+AGG 0.597326 8.1:+25822665 None:intergenic
CAGGCTTCCTGCCCAAAATG+TGG 0.597481 8.1:-25820345 MS.gene44486:CDS
TAATTGCAATGATGGCTAGA+GGG 0.601649 8.1:-25822220 MS.gene44486:CDS
AAAGGAGGCTTATCAGCTGA+AGG 0.602067 8.1:+25820056 None:intergenic
CTTCCACTGCTTCTCACCAG+AGG 0.605670 8.1:-25822307 MS.gene44486:CDS
GCAACGTCTTCGCGACAACA+TGG 0.607221 8.1:-25820300 MS.gene44486:CDS
AGGCTATTGCATCTATGCAT+CGG 0.609119 8.1:+25822405 None:intergenic
CACGTTGAAAAGGAGAAAGA+CGG 0.618103 8.1:+25819991 None:intergenic
GGTGATGATGCATTTAACAA+AGG 0.620143 8.1:+25820077 None:intergenic
AGCTTCCTATTCGTTACACA+AGG 0.621103 8.1:-25823075 MS.gene44486:intron
ACTAGTGAGCCTTATGCCAA+GGG 0.621863 8.1:-25821392 MS.gene44486:CDS
TCTTTGGGACGTGATATGAC+AGG 0.621876 8.1:+25819963 None:intergenic
TATCAGTAGCTATTTCAGCA+AGG 0.623206 8.1:+25821065 None:intergenic
GAACTCATCGATGTCCCTGG+TGG 0.647010 8.1:-25822680 MS.gene44486:CDS
TTGCACTAAAGAGCATGTCA+GGG 0.650226 8.1:-25822493 MS.gene44486:CDS
TTTCCAGAAGCTATCGCGAG+AGG 0.652729 8.1:-25820393 MS.gene44486:CDS
TGCACTAAAGAGCATGTCAG+GGG 0.667865 8.1:-25822492 MS.gene44486:CDS
TATTCACAGAAATCTCTGAG+AGG 0.685412 8.1:-25822161 MS.gene44486:CDS
CAAATGCTTCTGATCCACCA+GGG 0.727463 8.1:+25822666 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAAAAATAAAAGAGA+CGG - chr8.1:25823044-25823063 MS.gene44486:intron 10.0%
!!! TAATTATATTTTATCTTTAG+TGG + chr8.1:25821606-25821625 None:intergenic 10.0%
!! AATTCTAACTTTATTAGTAT+TGG + chr8.1:25822701-25822720 None:intergenic 15.0%
!! TAAATATAAGTTTATAAGTG+AGG + chr8.1:25821488-25821507 None:intergenic 15.0%
!! TCACTTATAAACTTATATTT+AGG - chr8.1:25821487-25821506 MS.gene44486:intron 15.0%
!!! CATGTATAATTTCTTTTATA+CGG - chr8.1:25821630-25821649 MS.gene44486:intron 15.0%
!!! TTTGTTTTTTTTATCAAGAA+TGG - chr8.1:25820306-25820325 MS.gene44486:CDS 15.0%
!! ATATCAAACTTACTAATCAT+AGG - chr8.1:25820143-25820162 MS.gene44486:CDS 20.0%
!! TTTAACAAAGGACTATTAAT+AGG + chr8.1:25823093-25823112 None:intergenic 20.0%
!!! AAAATTTTAAGATGGTATCA+GGG + chr8.1:25821413-25821432 None:intergenic 20.0%
!!! TTCATTTTCTCTTCTTAATT+TGG + chr8.1:25822998-25823017 None:intergenic 20.0%
!!! TTTCATATCAGTTTTGTTTT+AGG - chr8.1:25821073-25821092 MS.gene44486:CDS 20.0%
!!! TTTTTATCAAGAATGGTAAT+AGG - chr8.1:25820313-25820332 MS.gene44486:CDS 20.0%
! AAACACGTTAATATTAGCAT+CGG + chr8.1:25819984-25820003 None:intergenic 25.0%
! AAACTTTCCTTCTTTAGTAA+AGG + chr8.1:25821055-25821074 None:intergenic 25.0%
! AACTTTCCTTCTTTAGTAAA+GGG + chr8.1:25821054-25821073 None:intergenic 25.0%
! AATATCTCCATGATAAGATT+TGG + chr8.1:25821580-25821599 None:intergenic 25.0%
! AATATTATTCTTCTCCACAA+AGG - chr8.1:25822056-25822075 MS.gene44486:intron 25.0%
! AGTTAGAATTCAAAATTCTC+AGG - chr8.1:25822711-25822730 MS.gene44486:CDS 25.0%
! ATATTATTCTTCTCCACAAA+GGG - chr8.1:25822057-25822076 MS.gene44486:intron 25.0%
! ATTTACGACAACAATTTAGA+TGG - chr8.1:25822307-25822326 MS.gene44486:CDS 25.0%
! CTAATCATAGGATCATTAAA+TGG - chr8.1:25820155-25820174 MS.gene44486:CDS 25.0%
! TACCTGAAAATACCTTTATA+AGG + chr8.1:25821250-25821269 None:intergenic 25.0%
! TATAAGTTTATAAGTGAGGT+CGG + chr8.1:25821484-25821503 None:intergenic 25.0%
! TGAAAATACCTTTATAAGGT+AGG + chr8.1:25821246-25821265 None:intergenic 25.0%
! TGAAATGACAGAAGATTATT+CGG - chr8.1:25820621-25820640 MS.gene44486:intron 25.0%
! TGATAAGTAGTTCAATTCAA+AGG + chr8.1:25820033-25820052 None:intergenic 25.0%
! TTTCCATTAATTTCCAAAAG+TGG - chr8.1:25820418-25820437 MS.gene44486:CDS 25.0%
!! ACCATCTTAAAATTTTGGAT+TGG - chr8.1:25821417-25821436 MS.gene44486:CDS 25.0%
!! ATTGAATCTTGACTATTTTG+TGG - chr8.1:25821688-25821707 MS.gene44486:intron 25.0%
!! CCATCTTAAAATTTTGGATT+GGG - chr8.1:25821418-25821437 MS.gene44486:CDS 25.0%
!! CTGATACCATCTTAAAATTT+TGG - chr8.1:25821412-25821431 MS.gene44486:CDS 25.0%
!! TGCTAATATTAACGTGTTTT+TGG - chr8.1:25819985-25820004 MS.gene44486:CDS 25.0%
!! TGTGAATAAAGCATTATGAT+AGG + chr8.1:25821006-25821025 None:intergenic 25.0%
!! TTCTGTCATTTCAAGATATT+CGG + chr8.1:25820614-25820633 None:intergenic 25.0%
!!! CAAAATTTTAAGATGGTATC+AGG + chr8.1:25821414-25821433 None:intergenic 25.0%
!!! TACCTTATAAAGGTATTTTC+AGG - chr8.1:25821245-25821264 MS.gene44486:CDS 25.0%
AAATCAAGAACAACAGAAGA+TGG - chr8.1:25822187-25822206 MS.gene44486:CDS 30.0%
AATCAAGGTTCATTGTATTC+AGG - chr8.1:25822559-25822578 MS.gene44486:CDS 30.0%
ACATCGATGAGTTCAATTAA+TGG + chr8.1:25820493-25820512 None:intergenic 30.0%
ACTAAGAAACAACCAATATG+CGG + chr8.1:25820280-25820299 None:intergenic 30.0%
AGATTATTCGGTTGGAAATT+TGG - chr8.1:25820633-25820652 MS.gene44486:intron 30.0%
AGTACTATCCTTGAAATCAA+AGG - chr8.1:25822630-25822649 MS.gene44486:CDS 30.0%
AGTTTCTGATGTATGTAAGA+TGG + chr8.1:25820719-25820738 None:intergenic 30.0%
CAATTTGTGTTCGAAAATTG+TGG + chr8.1:25822419-25822438 None:intergenic 30.0%
CATCTAACCAAATCTTATCA+TGG - chr8.1:25821570-25821589 MS.gene44486:intron 30.0%
GACGATTATTGAGTATAACA+AGG - chr8.1:25822368-25822387 MS.gene44486:CDS 30.0%
GAGTAATGAATTCTAGTACT+TGG - chr8.1:25820179-25820198 MS.gene44486:CDS 30.0%
GGGTTTAAAAATTGTGGTAT+TGG - chr8.1:25820979-25820998 MS.gene44486:CDS 30.0%
GTAAGTTCAATTCTGTTATG+TGG + chr8.1:25822607-25822626 None:intergenic 30.0%
GTTAAAGCAGTTCAAATATG+TGG - chr8.1:25822329-25822348 MS.gene44486:CDS 30.0%
TACCTTAAGATAGATGTCTA+TGG + chr8.1:25822224-25822243 None:intergenic 30.0%
TCGAAAATTGTGGTTAAGTA+TGG + chr8.1:25822409-25822428 None:intergenic 30.0%
TGAATTGAACTACTTATCAC+AGG - chr8.1:25820034-25820053 MS.gene44486:CDS 30.0%
! AAGAGTTCTAATTGCAATGA+TGG - chr8.1:25820951-25820970 MS.gene44486:intron 30.0%
! AATTTTGCTTTCTCTGCAAT+TGG + chr8.1:25820748-25820767 None:intergenic 30.0%
! AGTTAAGTCCTTTGATTTCA+AGG + chr8.1:25822641-25822660 None:intergenic 30.0%
! CAATTTTCGAACACAAATTG+TGG - chr8.1:25822419-25822438 MS.gene44486:CDS 30.0%
! CTTCTTTTTCCTTCATTTAG+CGG - chr8.1:25821944-25821963 MS.gene44486:intron 30.0%
! GAGTTTAGGAATGATTTTCT+AGG + chr8.1:25823150-25823169 None:intergenic 30.0%
! GATTTTCAACTTCAGATTCA+AGG + chr8.1:25822018-25822037 None:intergenic 30.0%
! TCTGTTGTTCTTGATTTTTC+AGG + chr8.1:25822184-25822203 None:intergenic 30.0%
!! CCCAATCCAAAATTTTAAGA+TGG + chr8.1:25821421-25821440 None:intergenic 30.0%
!! TGTCCACTTTTGGAAATTAA+TGG + chr8.1:25820424-25820443 None:intergenic 30.0%
AAAGAAAGTTTGCAGCATGA+TGG - chr8.1:25822762-25822781 MS.gene44486:CDS 35.0%
AACTACCTTGTGTAACGAAT+AGG + chr8.1:25820112-25820131 None:intergenic 35.0%
AACTCAGTTTCCAGAAAACT+TGG - chr8.1:25823165-25823184 MS.gene44486:intron 35.0%
AATCTGTGTTCGCATATAGA+TGG + chr8.1:25822473-25822492 None:intergenic 35.0%
AGCCATAGACATCTATCTTA+AGG - chr8.1:25822219-25822238 MS.gene44486:CDS 35.0%
AGTGATGTCGCTATTCAAAT+TGG - chr8.1:25820078-25820097 MS.gene44486:CDS 35.0%
ATAAGGTAGGTAGTTAGGTA+AGG + chr8.1:25821233-25821252 None:intergenic 35.0%
ATATGCGAACACAGATTAGA+TGG - chr8.1:25822476-25822495 MS.gene44486:CDS 35.0%
ATGACAGAAGATTATTCGGT+TGG - chr8.1:25820625-25820644 MS.gene44486:intron 35.0%
ATTAGAAGCTGGATAAAGCT+CGG + chr8.1:25822968-25822987 None:intergenic 35.0%
ATTTGAATAGCGACATCACT+TGG + chr8.1:25820078-25820097 None:intergenic 35.0%
CCTAACTACCTACCTTATAA+AGG - chr8.1:25821235-25821254 MS.gene44486:CDS 35.0%
CCTTTATAAGGTAGGTAGTT+AGG + chr8.1:25821238-25821257 None:intergenic 35.0%
CTTTCTTTCACTATGGCTTA+AGG - chr8.1:25821832-25821851 MS.gene44486:intron 35.0%
GCTAGAGGGTTTAAAAATTG+TGG - chr8.1:25820973-25820992 MS.gene44486:CDS 35.0%
GTAAGTCCCTTTACTAAAGA+AGG - chr8.1:25821045-25821064 MS.gene44486:CDS 35.0%
GTCTCATATTCGATGAGTTA+AGG + chr8.1:25821513-25821532 None:intergenic 35.0%
GTTAACGACGTTCAAAATCA+AGG - chr8.1:25822544-25822563 MS.gene44486:CDS 35.0%
TAATTGCAATGATGGCTAGA+GGG - chr8.1:25820959-25820978 MS.gene44486:intron 35.0%
TATTCACAGAAATCTCTGAG+AGG - chr8.1:25821018-25821037 MS.gene44486:CDS 35.0%
TATTCGGTTGGAAATTTGGT+AGG - chr8.1:25820637-25820656 MS.gene44486:intron 35.0%
TCAGCAAGACAGATAAACTT+TGG + chr8.1:25822154-25822173 None:intergenic 35.0%
TCTAGTTGTATGCGATCTTT+GGG + chr8.1:25823234-25823253 None:intergenic 35.0%
TTTCCAAAAGTGGACACATA+AGG - chr8.1:25820428-25820447 MS.gene44486:CDS 35.0%
! AATGATTTTCTAGGCAAAGG+AGG + chr8.1:25823141-25823160 None:intergenic 35.0%
! AGAGAATTTGAGCCACATTT+TGG + chr8.1:25822849-25822868 None:intergenic 35.0%
! AGGAATGATTTTCTAGGCAA+AGG + chr8.1:25823144-25823163 None:intergenic 35.0%
! ATTTAGTTGACTCCGCATAT+TGG - chr8.1:25820265-25820284 MS.gene44486:CDS 35.0%
! CGTCGTTAACTATGATTTTC+AGG + chr8.1:25822534-25822553 None:intergenic 35.0%
! GAGAATTTGAGCCACATTTT+GGG + chr8.1:25822848-25822867 None:intergenic 35.0%
! GGTGATGATGCATTTAACAA+AGG + chr8.1:25823105-25823124 None:intergenic 35.0%
! TATCAGTAGCTATTTCAGCA+AGG + chr8.1:25822117-25822136 None:intergenic 35.0%
! TCGCATATAGATGGTTTTTC+AGG + chr8.1:25822464-25822483 None:intergenic 35.0%
!! GTTTTCTGGAAACTGAGTTT+AGG + chr8.1:25823164-25823183 None:intergenic 35.0%
AATAGCCTACATAGCTTCCA+AGG - chr8.1:25820789-25820808 MS.gene44486:intron 40.0%
ACAAGTGAATGGTATGCTTC+TGG - chr8.1:25819929-25819948 MS.gene44486:CDS 40.0%
AGAAGGATAGCCATGCAATT+AGG - chr8.1:25821902-25821921 MS.gene44486:intron 40.0%
AGCTTCCTATTCGTTACACA+AGG - chr8.1:25820104-25820123 MS.gene44486:CDS 40.0%
AGGAGATATTTGGAAAGGGA+AGG - chr8.1:25821714-25821733 MS.gene44486:intron 40.0%
AGGCTATTGCATCTATGCAT+CGG + chr8.1:25820777-25820796 None:intergenic 40.0%
CAATCCAGATGTCCACTTAT+AGG + chr8.1:25821207-25821226 None:intergenic 40.0%
CACGTTGAAAAGGAGAAAGA+CGG + chr8.1:25823191-25823210 None:intergenic 40.0%
CTAATTGCAATGATGGCTAG+AGG - chr8.1:25820958-25820977 MS.gene44486:intron 40.0%
CTAGCAGCAAAGTTCTGTTA+CGG - chr8.1:25820538-25820557 MS.gene44486:intron 40.0%
CTGTCAGCTTTCTTTCACTA+TGG - chr8.1:25821825-25821844 MS.gene44486:intron 40.0%
GAGCAGGAGATATTTGGAAA+GGG - chr8.1:25821710-25821729 MS.gene44486:intron 40.0%
GAGTATACATTCCTCTTGTC+AGG + chr8.1:25822943-25822962 None:intergenic 40.0%
GATATTGCATTCTTCTCCCT+TGG + chr8.1:25821806-25821825 None:intergenic 40.0%
GATATTTGGAAAGGGAAGGA+AGG - chr8.1:25821718-25821737 MS.gene44486:intron 40.0%
GCTTTATCCAGCTTCTAATG+AGG - chr8.1:25822969-25822988 MS.gene44486:intron 40.0%
GTCTAGTTGTATGCGATCTT+TGG + chr8.1:25823235-25823254 None:intergenic 40.0%
GTGTGTTAAGAGTCACACAT+TGG + chr8.1:25821282-25821301 None:intergenic 40.0%
TAACCTGAGTAGCCTATAAG+TGG - chr8.1:25821192-25821211 MS.gene44486:CDS 40.0%
TAGCAGCAAAGTTCTGTTAC+GGG - chr8.1:25820539-25820558 MS.gene44486:intron 40.0%
TCTTACAGAACCAGCTTGTA+AGG - chr8.1:25821452-25821471 MS.gene44486:CDS 40.0%
TTATCACAGGAATTACTCCC+AGG - chr8.1:25820047-25820066 MS.gene44486:CDS 40.0%
TTGGAAACCTCATTAGAAGC+TGG + chr8.1:25822979-25822998 None:intergenic 40.0%
TTTGTGGAGCAGGAGATATT+TGG - chr8.1:25821704-25821723 MS.gene44486:intron 40.0%
! ACAGAAATCTCTGAGAGGTT+TGG - chr8.1:25821023-25821042 MS.gene44486:CDS 40.0%
! CTCCACAAAGGGACATTTTT+CGG - chr8.1:25822068-25822087 MS.gene44486:intron 40.0%
! CTTCCTTATGTGTCCACTTT+TGG + chr8.1:25820434-25820453 None:intergenic 40.0%
! TCCACAAAGGGACATTTTTC+GGG - chr8.1:25822069-25822088 MS.gene44486:intron 40.0%
! TCTTGACTATTTTGTGGAGC+AGG - chr8.1:25821694-25821713 MS.gene44486:intron 40.0%
! TGTTGCTTGCAGGTTTGATT+TGG - chr8.1:25821877-25821896 MS.gene44486:intron 40.0%
! TTGCACTAAAGAGCATGTCA+GGG - chr8.1:25820686-25820705 MS.gene44486:intron 40.0%
!!! AAAGGGACATTTTTCGGGTT+GGG - chr8.1:25822074-25822093 MS.gene44486:intron 40.0%
!!! GTCCTGTTTTGGTTGTGAAA+GGG + chr8.1:25821319-25821338 None:intergenic 40.0%
!!! TCCTGTTTTGGTTGTGAAAG+GGG + chr8.1:25821318-25821337 None:intergenic 40.0%
!!! TGTCCTGTTTTGGTTGTGAA+AGG + chr8.1:25821320-25821339 None:intergenic 40.0%
!!! TTTTCGGGTTGGGAAGTTAT+TGG - chr8.1:25822084-25822103 MS.gene44486:intron 40.0%
AAAGGAGGCTTATCAGCTGA+AGG + chr8.1:25823126-25823145 None:intergenic 45.0%
AATGTCCAATCCTGAGTGTG+AGG + chr8.1:25821165-25821184 None:intergenic 45.0%
ACAGGATGAGCACGTTGAAA+AGG + chr8.1:25823201-25823220 None:intergenic 45.0%
ACCCCTTTCACAACCAAAAC+AGG - chr8.1:25821314-25821333 MS.gene44486:CDS 45.0%
ACCCGAAAAATGTCCCTTTG+TGG + chr8.1:25822073-25822092 None:intergenic 45.0%
ACTCTCCTTGGAAGCTATGT+AGG + chr8.1:25820797-25820816 None:intergenic 45.0%
AGGGTCTATCCAAGATCTGT+TGG + chr8.1:25821394-25821413 None:intergenic 45.0%
ATGTCCAATCCTGAGTGTGA+GGG + chr8.1:25821164-25821183 None:intergenic 45.0%
ATTGAACTCATCGATGTCCC+TGG - chr8.1:25820496-25820515 MS.gene44486:intron 45.0%
CAAATGCTTCTGATCCACCA+GGG + chr8.1:25820516-25820535 None:intergenic 45.0%
CACACTCAGGATTGGACATT+TGG - chr8.1:25821165-25821184 MS.gene44486:CDS 45.0%
CATTCTTCTCCCTTGGCATA+AGG + chr8.1:25821799-25821818 None:intergenic 45.0%
CTCTTCTCATGCTCTTCATG+TGG + chr8.1:25821752-25821771 None:intergenic 45.0%
GACATCACTTGGAATCTCCT+GGG + chr8.1:25820067-25820086 None:intergenic 45.0%
GATAGCCATGCAATTAGGAC+AGG - chr8.1:25821907-25821926 MS.gene44486:intron 45.0%
GGAGCAGGAGATATTTGGAA+AGG - chr8.1:25821709-25821728 MS.gene44486:intron 45.0%
GGGTCTATCCAAGATCTGTT+GGG + chr8.1:25821393-25821412 None:intergenic 45.0%
GGTTCATCTGGTAGAAGTGA+TGG - chr8.1:25820823-25820842 MS.gene44486:intron 45.0%
GTAGCCTATAAGTGGACATC+TGG - chr8.1:25821200-25821219 MS.gene44486:CDS 45.0%
TAATACACTCCCTCACACTC+AGG - chr8.1:25821152-25821171 MS.gene44486:CDS 45.0%
TCAAATGCTTCTGATCCACC+AGG + chr8.1:25820517-25820536 None:intergenic 45.0%
TGTCCACTTATAGGCTACTC+AGG + chr8.1:25821198-25821217 None:intergenic 45.0%
! ACTAGTGAGCCTTATGCCAA+GGG - chr8.1:25821787-25821806 MS.gene44486:intron 45.0%
! AGAAAGACGGCCAAGTTTTC+TGG + chr8.1:25823178-25823197 None:intergenic 45.0%
! ATTTGAGCCACATTTTGGGC+AGG + chr8.1:25822844-25822863 None:intergenic 45.0%
! GAGAGTCAGTTTGGTTCATC+TGG - chr8.1:25820811-25820830 MS.gene44486:intron 45.0%
! GTTGCACTAAAGAGCATGTC+AGG - chr8.1:25820685-25820704 MS.gene44486:intron 45.0%
! TACTAGTGAGCCTTATGCCA+AGG - chr8.1:25821786-25821805 MS.gene44486:intron 45.0%
! TGAACCAAACTGACTCTCCT+TGG + chr8.1:25820809-25820828 None:intergenic 45.0%
! TGCACTAAAGAGCATGTCAG+GGG - chr8.1:25820687-25820706 MS.gene44486:intron 45.0%
! TTGAGACAACTGTTGCTTGC+AGG - chr8.1:25821867-25821886 MS.gene44486:intron 45.0%
!! CTGTCTGTGTCGCTTACAAA+TGG + chr8.1:25822742-25822761 None:intergenic 45.0%
!! GCAGGTTTGATTTGGAGAGA+AGG - chr8.1:25821885-25821904 MS.gene44486:intron 45.0%
!! TCTTTGGGACGTGATATGAC+AGG + chr8.1:25823219-25823238 None:intergenic 45.0%
!! TTCCTCTTGTCAGGTGTTGA+AGG + chr8.1:25822934-25822953 None:intergenic 45.0%
!! TTTTGGTTGTGAAAGGGGTG+GGG + chr8.1:25821313-25821332 None:intergenic 45.0%
!!! CAAAGGGACATTTTTCGGGT+TGG - chr8.1:25822073-25822092 MS.gene44486:intron 45.0%
!!! GTTTTGGTTGTGAAAGGGGT+GGG + chr8.1:25821314-25821333 None:intergenic 45.0%
!!! TGTTTTGGTTGTGAAAGGGG+TGG + chr8.1:25821315-25821334 None:intergenic 45.0%
CACACGCTGCACAAAACAAC+AGG - chr8.1:25822815-25822834 MS.gene44486:intron 50.0%
CACAGCCTGTCCTAATTGCA+TGG + chr8.1:25821915-25821934 None:intergenic 50.0%
CATCGCTATGCTTCGATGTG+TGG - chr8.1:25820588-25820607 MS.gene44486:intron 50.0%
CGACATCACTTGGAATCTCC+TGG + chr8.1:25820068-25820087 None:intergenic 50.0%
GAAAGTGGCCCAACAGATCT+TGG - chr8.1:25821382-25821401 MS.gene44486:CDS 50.0%
GATGTGTCGCCGCTAAATGA+AGG + chr8.1:25821956-25821975 None:intergenic 50.0%
GCAATTAGGACAGGCTGTGT+TGG - chr8.1:25821916-25821935 MS.gene44486:intron 50.0%
GCTTCCAAGGAGAGTCAGTT+TGG - chr8.1:25820802-25820821 MS.gene44486:intron 50.0%
TCGGTCTTCACCTTACAAGC+TGG + chr8.1:25821465-25821484 None:intergenic 50.0%
TTTCCAGAAGCTATCGCGAG+AGG - chr8.1:25822786-25822805 MS.gene44486:intron 50.0%
TTTGGTTGTGAAAGGGGTGG+GGG + chr8.1:25821312-25821331 None:intergenic 50.0%
! ACGCGCTAGATGTCCTGTTT+TGG + chr8.1:25821330-25821349 None:intergenic 50.0%
!! ATGGATGGTACTGGCAGCAT+AGG - chr8.1:25822898-25822917 MS.gene44486:intron 50.0%
ACCAGAGGCCAGCTGTTGAT+TGG - chr8.1:25820887-25820906 MS.gene44486:intron 55.0%
AGGACATCTAGCGCGTGACA+CGG - chr8.1:25821334-25821353 MS.gene44486:CDS 55.0%
CACTCCCTCACACTCAGGAT+TGG - chr8.1:25821157-25821176 MS.gene44486:CDS 55.0%
CAGGCTTCCTGCCCAAAATG+TGG - chr8.1:25822834-25822853 MS.gene44486:intron 55.0%
CGCCTTCAACACCTGACAAG+AGG - chr8.1:25822929-25822948 MS.gene44486:intron 55.0%
CGCGACAACATGGATGGTAC+TGG - chr8.1:25822889-25822908 MS.gene44486:intron 55.0%
CGTCTTCGCGACAACATGGA+TGG - chr8.1:25822883-25822902 MS.gene44486:intron 55.0%
CTTCCACTGCTTCTCACCAG+AGG - chr8.1:25820872-25820891 MS.gene44486:intron 55.0%
GAACTCATCGATGTCCCTGG+TGG - chr8.1:25820499-25820518 MS.gene44486:intron 55.0%
GCAACGTCTTCGCGACAACA+TGG - chr8.1:25822879-25822898 MS.gene44486:intron 55.0%
TCAGCCCACCAATCAACAGC+TGG + chr8.1:25820898-25820917 None:intergenic 55.0%
TTGGTTGTGAAAGGGGTGGG+GGG + chr8.1:25821311-25821330 None:intergenic 55.0%
! ACCAATCAACAGCTGGCCTC+TGG + chr8.1:25820891-25820910 None:intergenic 55.0%
! AGAGGCCAGCTGTTGATTGG+TGG - chr8.1:25820890-25820909 MS.gene44486:intron 55.0%
! CTGTTGGGCCACTTTCTACC+AGG + chr8.1:25821378-25821397 None:intergenic 55.0%
! GAGGCCAGCTGTTGATTGGT+GGG - chr8.1:25820891-25820910 MS.gene44486:intron 55.0%
! GCCACTTTCTACCAGGTCAC+GGG + chr8.1:25821371-25821390 None:intergenic 55.0%
! GGCCACTTTCTACCAGGTCA+CGG + chr8.1:25821372-25821391 None:intergenic 55.0%
GCCCGTGACCTGGTAGAAAG+TGG - chr8.1:25821367-25821386 MS.gene44486:CDS 60.0%
GGACATCTAGCGCGTGACAC+GGG - chr8.1:25821335-25821354 MS.gene44486:CDS 60.0%
TCTACCAGGTCACGGGCAAC+TGG + chr8.1:25821364-25821383 None:intergenic 60.0%
TGGCCTCTGGTGAGAAGCAG+TGG + chr8.1:25820878-25820897 None:intergenic 60.0%
GCGCCTCTCGCGATAGCTTC+TGG + chr8.1:25822792-25822811 None:intergenic 65.0%
GTAGCCAGTTGCCCGTGACC+TGG - chr8.1:25821357-25821376 MS.gene44486:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 25819920 25823281 25819920 ID=MS.gene44486
chr8.1 mRNA 25819920 25823281 25819920 ID=MS.gene44486.t1;Parent=MS.gene44486
chr8.1 exon 25823262 25823281 25823262 ID=MS.gene44486.t1.exon1;Parent=MS.gene44486.t1
chr8.1 CDS 25823262 25823281 25823262 ID=cds.MS.gene44486.t1;Parent=MS.gene44486.t1
chr8.1 exon 25823076 25823145 25823076 ID=MS.gene44486.t1.exon2;Parent=MS.gene44486.t1
chr8.1 CDS 25823076 25823145 25823076 ID=cds.MS.gene44486.t1;Parent=MS.gene44486.t1
chr8.1 exon 25822157 25822783 25822157 ID=MS.gene44486.t1.exon3;Parent=MS.gene44486.t1
chr8.1 CDS 25822157 25822783 25822157 ID=cds.MS.gene44486.t1;Parent=MS.gene44486.t1
chr8.1 exon 25820961 25821485 25820961 ID=MS.gene44486.t1.exon4;Parent=MS.gene44486.t1
chr8.1 CDS 25820961 25821485 25820961 ID=cds.MS.gene44486.t1;Parent=MS.gene44486.t1
chr8.1 exon 25819920 25820468 25819920 ID=MS.gene44486.t1.exon5;Parent=MS.gene44486.t1
chr8.1 CDS 25819920 25820468 25819920 ID=cds.MS.gene44486.t1;Parent=MS.gene44486.t1
Gene Sequence

>MS.gene44486

ATGAAGAGAACAAGTGAATGGTATGCTTCTGGTTTTCTAAAACTCTTGTAACGTGCATTTGCCGATGCTAATATTAACGTGTTTTTGGATATGATGAAAACTACTGAAAACCTTTGAATTGAACTACTTATCACAGGAATTACTCCCAGGAGATTCCAAGTGATGTCGCTATTCAAATTGGTGAAGCTTCCTATTCGTTACACAAGGTAGTTAACAAACTCAAATATCAAACTTACTAATCATAGGATCATTAAATGGAGAGTAATGAATTCTAGTACTTGGTTATGTTTTTTCAATAAATTCTTTGCGCTTTTTATGATACAACATTATGATGTCGTTTGATCTATTTAGTTGACTCCGCATATTGGTTGTTTCTTAGTTTTTTCTTTGTTTTTTTTATCAAGAATGGTAATAGGAAGATATAAGTGATATATGTATAGACATAGTACTTAAAGCTGTTTATTGAATTATTTTTCATAACTCTAATGTAATTTGCAGTTTCCATTAATTTCCAAAAGTGGACACATAAGGAAGCTCATTTCAGAATCTAGTGATGCTGTTGCTGATGTTCCATTAATTGAACTCATCGATGTCCCTGGTGGATCAGAAGCATTTGAACTAGCAGCAAAGTTCTGTTACGGGATAAACTTCGAAATAAGCGTTGAAAACATCGCTATGCTTCGATGTGTGGCCGAATATCTTGAAATGACAGAAGATTATTCGGTTGGAAATTTGGTAGGAAGAACTGATTCTTACTTGAATGAAGTTGCACTAAAGAGCATGTCAGGGGCAATATCCATCTTACATACATCAGAAACTCTTCTTCCAATTGCAGAGAAAGCAAAATTAGTTAGCCGATGCATAGATGCAATAGCCTACATAGCTTCCAAGGAGAGTCAGTTTGGTTCATCTGGTAGAAGTGATGGTAGTAGCAATGAAAGAGTGATGAATTCTTCCACTGCTTCTCACCAGAGGCCAGCTGTTGATTGGTGGGCTGAAGATCTAACTGTTCTTAGAATTGACTTTTTTCAAAGAGTTCTAATTGCAATGATGGCTAGAGGGTTTAAAAATTGTGGTATTGGTCCTATCATAATGCTTTATTCACAGAAATCTCTGAGAGGTTTGGTAAGTCCCTTTACTAAAGAAGGAAAGTTTTCATATCAGTTTTGTTTTAGGTTTTATTTACAAGTTTTGTTATTTTTACGCACAAATTTAATATCAAATTTCGACAATAATACACTCCCTCACACTCAGGATTGGACATTTGGATTGTAACCTGAGTAGCCTATAAGTGGACATCTGGATTGTAACCTTACCTAACTACCTACCTTATAAAGGTATTTTCAGGTAATATCGTATCCAATGTGTGACTCTTAACACACACATAACCCCCCACCCCTTTCACAACCAAAACAGGACATCTAGCGCGTGACACGGGTAGCCAGTTGCCCGTGACCTGGTAGAAAGTGGCCCAACAGATCTTGGATAGACCCTGATACCATCTTAAAATTTTGGATTGGGCATAACTTGACTCTTACAGAACCAGCTTGTAAGGTGAAGACCGACCTCACTTATAAACTTATATTTAGGCCTTAACTCATCGAATATGAGACTCTTAACACTAGTATTGTTAAAAAAGTTTTACACATACATCTAACCAAATCTTATCATGGAGATATTTGTCCACTAAAGATAAAATATAATTAGATGCATGTATAATTTCTTTTATACGGTTAGTATATATGATATTAAACTCTTTGTTTTACATATTGAATCTTGACTATTTTGTGGAGCAGGAGATATTTGGAAAGGGAAGGAAGGATATAGAACCACATGAAGAGCATGAGAAGAGACTTATTTTAGAGATACTAGTGAGCCTTATGCCAAGGGAGAAGAATGCAATATCTGTCAGCTTTCTTTCACTATGGCTTAAGGCAGCAATATATCTTGAGACAACTGTTGCTTGCAGGTTTGATTTGGAGAGAAGGATAGCCATGCAATTAGGACAGGCTGTGTTGGATGATCTTCTTTTTCCTTCATTTAGCGGCGACACATCGTTTGATGTAGAAACAGTGCAAAGAATCATGATGAATTACCTTGAATCTGAAGTTGAAAATCATTATAATGCAGATGATGAATATTATTCTTCTCCACAAAGGGACATTTTTCGGGTTGGGAAGTTATTGGAGAGTTACCTTGCTGAAATAGCTACTGATAGAAATTTATCAGTTCCAAAGTTTATCTGTCTTGCTGAATTAATGCCTGAAAAATCAAGAACAACAGAAGATGGAATGTATAGAGCCATAGACATCTATCTTAAGGTACAGTTCTATACTAATATTGTTGCTTTGTAAAAGAAAATATTTTTAAACACAAATTTGACATTAATTTACGACAACAATTTAGATGGTTAAAGCAGTTCAAATATGTGGTTGATTATATTCAATAGACGATTATTGAGTATAACAAGGTGTTTAACTCATTAACCATACTTAACCACAATTTTCGAACACAAATTGTGGTTAATAAATATCTTGAACACCTGAAAAACCATCTATATGCGAACACAGATTAGATGGTTAATAATGAATATGTTATATATATTTGAACACCTGAAAATCATAGTTAACGACGTTCAAAATCAAGGTTCATTGTATTCAGGTGATGATTAATGAGTTGTGACACCACATAACAGAATTGAACTTACAGTAGTACTATCCTTGAAATCAAAGGACTTAACTCTAGAATTCTTCTCATATATAAAATTCTTATTACAAACCAATACTAATAAAGTTAGAATTCAAAATTCTCAGGCACATCCATTTGTAAGCGACACAGACAGAAAGAAAGTTTGCAGCATGATGGATTTCCAGAAGCTATCGCGAGAGGCGCGAACACACGCTGCACAAAACAACAGGCTTCCTGCCCAAAATGTGGCTCAAATTCTCTATTATGAGCAGCAACGTCTTCGCGACAACATGGATGGTACTGGCAGCATAGGTTCAGATTCGCCTTCAACACCTGACAAGAGGAATGTATACTCATCCGAGCTTTATCCAGCTTCTAATGAGGTTTCCAAATTAAGAAGAGAAAATGAAGAACTGAAATTAGAGATAGTGAAACTTAAAATAAAAATAAAAGAGACGGAAAAATGTACAGTAATTGAATCACCTATTAATAGTCCTTTGTTAAATGCATCATCACCTTCAGCTGATAAGCCTCCTTTGCCTAGAAAATCATTCCTAAACTCAGTTTCCAGAAAACTTGGCCGTCTTTCTCCTTTTCAACGTGCTCATCCTGTCATATCACGTCCCAAAGATCGCATACAACTAGACAAGATTCGACGCTACTCCATATCCTAA
Protein sequence

>MS.gene44486.t1

MKRTSEWNYSQEIPSDVAIQIGEASYSLHKFPLISKSGHIRKLISESSDAVADVPLIELIDVPGGSEAFELAAKFCYGINFEISVENIAMLRCVAEYLEMTEDYSVGNLVGRTDSYLNEVALKSMSGAISILHTSETLLPIAEKAKLVSRCIDAIAYIASKESQFGSSGRSDGSSNERVMNSSTASHQRPAVDWWAEDLTVLRIDFFQRVLIAMMARGFKNCGIGPIIMLYSQKSLRGLEIFGKGRKDIEPHEEHEKRLILEILVSLMPREKNAISVSFLSLWLKAAIYLETTVACRFDLERRIAMQLGQAVLDDLLFPSFSGDTSFDVETVQRIMMNYLESEVENHYNADDEYYSSPQRDIFRVGKLLESYLAEIATDRNLSVPKFICLAELMPEKSRTTEDGMYRAIDIYLKAHPFVSDTDRKKVCSMMDFQKLSREARTHAAQNNRLPAQNVAQILYYEQQRLRDNMDGTGSIGSDSPSTPDKRNVYSSELYPASNEVSKLRRENEELKLEIVKLKIKIKETEKCTVIESPINSPLLNASSPSADKPPLPRKSFLNSVSRKLGRLSPFQRAHPVISRPKDRIQLDKIRRYSIS