AlfalfaGEDB | MS.gene44993

M. sativa cultivar XinJiangDaYe / MS.gene44993

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44993.t1	XP_003629527.2	95.2	63	2	1	2	63	748	810	1.30E-21	112.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44993.t1	G7LFL6	95.2	63	2	1	2	63	748	810	9.3e-22	112.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056166	MS.gene44993	0.806942	6.44E-50	-1.69E-46
MS.gene060482	MS.gene44993	0.81308	3.07E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44993.t1	MTR_8g078480	95.238	63	2	1	2	63	748	810	5.64e-33	119

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 25 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTTTGTTTCAGCGGAATA+TGG	0.239058	8.1:-28969602	MS.gene44993:CDS
GGTTCTACACTCATCAGATT+TGG	0.285290	8.1:-28969554	MS.gene44993:CDS
CATAAAATGCATATTATAGA+AGG	0.369895	8.1:+28969252	None:intergenic
GTTCTACACTCATCAGATTT+GGG	0.374497	8.1:-28969553	MS.gene44993:CDS
GCTTTGTTTCAGCGGAATAT+GGG	0.430862	8.1:-28969601	MS.gene44993:CDS
AGATTTGGGACCAATACTTC+TGG	0.476436	8.1:-28969539	MS.gene44993:intron
ACCACCTATCTCCGATTCAA+TGG	0.489419	8.1:-28969651	MS.gene44993:CDS
TTTATGGAAGGAAAGGAAGA+AGG	0.491584	8.1:-28969235	MS.gene44993:CDS
ATAGCCATTGAATCGGAGAT+AGG	0.497156	8.1:+28969647	None:intergenic
GTCATCAATAGCCATTGAAT+CGG	0.515256	8.1:+28969640	None:intergenic
AGGAAAGGAAGAAGGGAATA+CGG	0.522046	8.1:-28969227	MS.gene44993:CDS
TTATGGAAGGAAAGGAAGAA+GGG	0.523632	8.1:-28969234	MS.gene44993:CDS
TGACACGTCACCAGAAGTAT+TGG	0.529079	8.1:+28969529	None:intergenic
ACTGGTACCTCTGCTGACAT+TGG	0.536380	8.1:-28969575	MS.gene44993:CDS
ACATGGCAGCTTTGTTTCAG+CGG	0.554817	8.1:-28969609	MS.gene44993:CDS
TCAGCGGAATATGGGACCAC+TGG	0.577941	8.1:-28969593	MS.gene44993:CDS
ATTGATGACACTTGTCGACA+TGG	0.588890	8.1:-28969626	MS.gene44993:CDS
AAAATGCATATTATAGAAGG+AGG	0.611064	8.1:+28969255	None:intergenic
CGTTAAAACCACCGTCTCTG+AGG	0.616452	8.1:-28969678	MS.gene44993:CDS
AGGTGGTGCCACCTCAGAGA+CGG	0.642679	8.1:+28969667	None:intergenic
GCCATTGAATCGGAGATAGG+TGG	0.643211	8.1:+28969650	None:intergenic
TAAAACCACCGTCTCTGAGG+TGG	0.650762	8.1:-28969675	MS.gene44993:CDS
TGGTGCCACCTCAGAGACGG+TGG	0.669040	8.1:+28969670	None:intergenic
TGTAGAACCAATGTCAGCAG+AGG	0.681174	8.1:+28969568	None:intergenic
AATGTCAGCAGAGGTACCAG+TGG	0.704765	8.1:+28969577	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTATATATTTATTTATAGG+TGG	-	chr8.1:28969571-28969590	MS.gene44993:CDS	15.0%
!!	CATAAAATGCATATTATAGA+AGG	+	chr8.1:28969680-28969699	None:intergenic	20.0%
!!!	CTTCTATAATATGCATTTTA+TGG	-	chr8.1:28969678-28969697	MS.gene44993:CDS	20.0%
!!!	TATAATATGCATTTTATGGA+AGG	-	chr8.1:28969682-28969701	MS.gene44993:CDS	20.0%
!	AAAATGCATATTATAGAAGG+AGG	+	chr8.1:28969677-28969696	None:intergenic	25.0%
!!	AGATAACATTTTTCCTCTTT+TGG	-	chr8.1:28969630-28969649	MS.gene44993:CDS	25.0%
!	AGAGAGAAAGATGTTTTATC+AGG	+	chr8.1:28969459-28969478	None:intergenic	30.0%
!	TATGCATTTTATGGAAGGAA+AGG	-	chr8.1:28969687-28969706	MS.gene44993:CDS	30.0%
!	TGTTTTATCAGGCAAATTTC+CGG	+	chr8.1:28969448-28969467	None:intergenic	30.0%
	GTCATCAATAGCCATTGAAT+CGG	+	chr8.1:28969292-28969311	None:intergenic	35.0%
	GTTCTACACTCATCAGATTT+GGG	-	chr8.1:28969376-28969395	MS.gene44993:intron	35.0%
	TCTTTCTCTCTTACAGACTT+TGG	-	chr8.1:28969468-28969487	MS.gene44993:intron	35.0%
	TTATGGAAGGAAAGGAAGAA+GGG	-	chr8.1:28969695-28969714	MS.gene44993:CDS	35.0%
	TTTATGGAAGGAAAGGAAGA+AGG	-	chr8.1:28969694-28969713	MS.gene44993:CDS	35.0%
	AGGAAAGGAAGAAGGGAATA+CGG	-	chr8.1:28969702-28969721	MS.gene44993:CDS	40.0%
	ATAGCCATTGAATCGGAGAT+AGG	+	chr8.1:28969285-28969304	None:intergenic	40.0%
	GGTTCTACACTCATCAGATT+TGG	-	chr8.1:28969375-28969394	MS.gene44993:intron	40.0%
	TTCTCTCTTACAGACTTTGG+AGG	-	chr8.1:28969471-28969490	MS.gene44993:intron	40.0%
!	AGATTTGGGACCAATACTTC+TGG	-	chr8.1:28969390-28969409	MS.gene44993:intron	40.0%
!	ATTGATGACACTTGTCGACA+TGG	-	chr8.1:28969303-28969322	MS.gene44993:intron	40.0%
!!	AGCTCTAGAGAAACCAAAAG+AGG	+	chr8.1:28969646-28969665	None:intergenic	40.0%
!!	AGCTTTGTTTCAGCGGAATA+TGG	-	chr8.1:28969327-28969346	MS.gene44993:intron	40.0%
!!	GCTTTGTTTCAGCGGAATAT+GGG	-	chr8.1:28969328-28969347	MS.gene44993:intron	40.0%
	ACCACCTATCTCCGATTCAA+TGG	-	chr8.1:28969278-28969297	MS.gene44993:intron	45.0%
	TGTAGAACCAATGTCAGCAG+AGG	+	chr8.1:28969364-28969383	None:intergenic	45.0%
!	GTGACGTGTCACTCACTTTA+GGG	-	chr8.1:28969412-28969431	MS.gene44993:intron	45.0%
!	TGACACGTCACCAGAAGTAT+TGG	+	chr8.1:28969403-28969422	None:intergenic	45.0%
!!	ACATGGCAGCTTTGTTTCAG+CGG	-	chr8.1:28969320-28969339	MS.gene44993:intron	45.0%
!!!	CTCAGAGACGGTGGTTTTAA+CGG	+	chr8.1:28969253-28969272	None:intergenic	45.0%
!!!	GTGGTTTTAACGGTGGAAGA+TGG	+	chr8.1:28969243-28969262	None:intergenic	45.0%
!!	AATACTATATATTTATTTAT+AGG	-	chr8.1:28969568-28969587	MS.gene44993:CDS	5.0%
	ACTGGTACCTCTGCTGACAT+TGG	-	chr8.1:28969354-28969373	MS.gene44993:intron	50.0%
	CGTTAAAACCACCGTCTCTG+AGG	-	chr8.1:28969251-28969270	MS.gene44993:CDS	50.0%
	GCCATTGAATCGGAGATAGG+TGG	+	chr8.1:28969282-28969301	None:intergenic	50.0%
!	GGTGACGTGTCACTCACTTT+AGG	-	chr8.1:28969411-28969430	MS.gene44993:intron	50.0%
!	TAAAACCACCGTCTCTGAGG+TGG	-	chr8.1:28969254-28969273	MS.gene44993:CDS	50.0%
!!	AATGTCAGCAGAGGTACCAG+TGG	+	chr8.1:28969355-28969374	None:intergenic	50.0%
!!!	AGAGACGGTGGTTTTAACGG+TGG	+	chr8.1:28969250-28969269	None:intergenic	50.0%
	TCAGCGGAATATGGGACCAC+TGG	-	chr8.1:28969336-28969355	MS.gene44993:intron	55.0%
!!	ACTTTAGGGCTACGTCACGC+CGG	-	chr8.1:28969426-28969445	MS.gene44993:intron	55.0%
!!	AGGTGGTGCCACCTCAGAGA+CGG	+	chr8.1:28969265-28969284	None:intergenic	60.0%
!!	TGGTGCCACCTCAGAGACGG+TGG	+	chr8.1:28969262-28969281	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	28969222	28969729	28969222	ID=MS.gene44993
chr8.1	mRNA	28969222	28969729	28969222	ID=MS.gene44993.t1;Parent=MS.gene44993
chr8.1	exon	28969540	28969729	28969540	ID=MS.gene44993.t1.exon1;Parent=MS.gene44993.t1
chr8.1	CDS	28969540	28969729	28969540	ID=cds.MS.gene44993.t1;Parent=MS.gene44993.t1
chr8.1	exon	28969222	28969289	28969222	ID=MS.gene44993.t1.exon2;Parent=MS.gene44993.t1
chr8.1	CDS	28969222	28969289	28969222	ID=cds.MS.gene44993.t1;Parent=MS.gene44993.t1

Gene Sequence

>MS.gene44993

ATGTTGTTGCAACAACAACCATCTTCCACCGTTAAAACCACCGTCTCTGAGGTGGCACCACCTATCTCCGATTCAATGGCTATTGATGACACTTGTCGACATGGCAGCTTTGTTTCAGCGGAATATGGGACCACTGGTACCTCTGCTGACATTGGTTCTACACTCATCAGATTTGGGACCAATACTTCTGGTGACGTGTCACTCACTTTAGGGCTACGTCACGCCGGAAATTTGCCTGATAAAACATCTTTCTCTCTTACAGACTTTGGAGGCATTTAATTTTTCATTCTAATCTATATATTATTATTATATTATTACTTCACATAATAATCATCATCATAATAATAATACTATATATTTATTTATAGGTGGCTGCTTTATTTATCAAGATTATGATATACTATCAAAAGATAACATTTTTCCTCTTTTGGTTTCTCTAGAGCTTTCATATTCCTCCTTCTATAATATGCATTTTATGGAAGGAAAGGAAGAAGGGAATACGGTTTGA

Protein sequence

>MS.gene44993.t1

MLLQQQPSSTVKTTVSEVAPPISDSMAIDDTCRHGSFVSAEYGTTGTSADIGSTLIRFGTNTSELSYSSFYNMHFMEGKEEGNTV