Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45037.t1 | XP_003590505.1 | 92.8 | 222 | 16 | 0 | 1 | 222 | 1 | 222 | 6.00E-119 | 436.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45037.t1 | P08688 | 43.7 | 222 | 117 | 6 | 6 | 220 | 7 | 227 | 1.4e-44 | 181.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45037.t1 | G7IBL7 | 92.8 | 222 | 16 | 0 | 1 | 222 | 1 | 222 | 4.3e-119 | 436.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060087 | MS.gene45037 | 0.812937 | 3.30E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45037.t1 | MTR_1g066530 | 92.793 | 222 | 16 | 0 | 1 | 222 | 1 | 222 | 6.38e-157 | 433 |
| MS.gene45037.t1 | MTR_6g073540 | 50.000 | 230 | 106 | 2 | 1 | 221 | 1 | 230 | 6.15e-75 | 226 |
| MS.gene45037.t1 | MTR_6g072470 | 52.000 | 200 | 90 | 1 | 1 | 194 | 1 | 200 | 8.47e-69 | 210 |
| MS.gene45037.t1 | MTR_6g048620 | 49.099 | 222 | 106 | 5 | 6 | 220 | 5 | 226 | 3.39e-66 | 204 |
| MS.gene45037.t1 | MTR_6g045077 | 45.000 | 220 | 116 | 3 | 4 | 220 | 3 | 220 | 2.27e-64 | 199 |
| MS.gene45037.t1 | MTR_6g048910 | 45.455 | 220 | 113 | 4 | 6 | 220 | 5 | 222 | 5.23e-64 | 198 |
| MS.gene45037.t1 | MTR_0695s0030 | 44.091 | 220 | 118 | 4 | 6 | 220 | 65 | 284 | 1.07e-62 | 197 |
| MS.gene45037.t1 | MTR_8g038100 | 44.545 | 220 | 117 | 4 | 6 | 220 | 281 | 500 | 1.60e-60 | 198 |
| MS.gene45037.t1 | MTR_8g038070 | 44.091 | 220 | 118 | 4 | 6 | 220 | 219 | 438 | 2.33e-60 | 196 |
| MS.gene45037.t1 | MTR_0057s0100 | 40.909 | 220 | 125 | 5 | 6 | 220 | 7 | 226 | 4.08e-53 | 171 |
| MS.gene45037.t1 | MTR_0057s0170 | 40.909 | 220 | 125 | 5 | 6 | 220 | 7 | 226 | 1.43e-52 | 169 |
| MS.gene45037.t1 | MTR_7g103990 | 41.704 | 223 | 120 | 8 | 6 | 220 | 7 | 227 | 3.12e-50 | 163 |
| MS.gene45037.t1 | MTR_0807s0020 | 37.054 | 224 | 131 | 5 | 1 | 220 | 1 | 218 | 8.13e-46 | 152 |
| MS.gene45037.t1 | MTR_6g040290 | 39.910 | 223 | 126 | 6 | 6 | 220 | 7 | 229 | 1.36e-45 | 151 |
| MS.gene45037.t1 | MTR_0216s0030 | 38.889 | 198 | 113 | 3 | 4 | 197 | 1 | 194 | 9.46e-45 | 148 |
| MS.gene45037.t1 | MTR_6g048880 | 41.011 | 178 | 95 | 5 | 50 | 220 | 4 | 178 | 1.69e-40 | 137 |
| MS.gene45037.t1 | MTR_7g012000 | 39.423 | 208 | 96 | 5 | 6 | 209 | 7 | 188 | 5.61e-39 | 134 |
| MS.gene45037.t1 | MTR_6g048810 | 50.355 | 141 | 64 | 4 | 84 | 220 | 162 | 300 | 4.89e-37 | 131 |
| MS.gene45037.t1 | MTR_4g102470 | 41.111 | 180 | 83 | 5 | 6 | 182 | 7 | 166 | 2.11e-34 | 122 |
| MS.gene45037.t1 | MTR_6g049040 | 40.876 | 137 | 64 | 3 | 1 | 135 | 1 | 122 | 5.56e-27 | 100 |
| MS.gene45037.t1 | MTR_6g049040 | 34.188 | 117 | 66 | 2 | 72 | 187 | 16 | 122 | 5.93e-16 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGGTACGGTCTTTGAAAA+TGG | 0.203555 | 1.2:+49979097 | MS.gene45037:CDS |
| TTTAACAACATGATCCTTCT+TGG | 0.249024 | 1.2:-49978720 | None:intergenic |
| ATCCGGAGAGAAATCTATTT+TGG | 0.256716 | 1.2:-49978864 | None:intergenic |
| ATTAGATCTAGAAGTTTAAT+AGG | 0.298795 | 1.2:-49979270 | None:intergenic |
| ACTCATTACTCCATTCGTTC+TGG | 0.346885 | 1.2:+49979061 | MS.gene45037:CDS |
| AATGGTGTTTCATCAAGAAA+TGG | 0.395080 | 1.2:-49978770 | None:intergenic |
| AACATGGTAGTGATTGGAAC+AGG | 0.421529 | 1.2:-49978911 | None:intergenic |
| GTTACGTGTTCAAGAAGAAA+AGG | 0.446425 | 1.2:+49978669 | MS.gene45037:CDS |
| CAATGGAACATGGTAGTGAT+TGG | 0.462327 | 1.2:-49978917 | None:intergenic |
| GAATAAGAAAACATTATGTC+CGG | 0.469058 | 1.2:-49978990 | None:intergenic |
| ACCGAAGCAATCATCTTCTC+TGG | 0.471293 | 1.2:+49978830 | MS.gene45037:CDS |
| TGCAGTTGTGAATTATGCTC+CGG | 0.478704 | 1.2:+49978694 | MS.gene45037:CDS |
| TGATGAAACACCATTTGAAA+AGG | 0.482259 | 1.2:+49978778 | MS.gene45037:CDS |
| TTTAATAGGCTCTGAAGTGC+AGG | 0.498729 | 1.2:-49979256 | None:intergenic |
| GCCAGAGAAGATGATTGCTT+CGG | 0.505795 | 1.2:-49978831 | None:intergenic |
| ACATGATCCTTCTTGGCTCC+CGG | 0.506537 | 1.2:-49978713 | None:intergenic |
| AACAGGGCCATCGAGTAAAT+CGG | 0.507747 | 1.2:-49978894 | None:intergenic |
| AGTAAATCGGTATCTGAATC+CGG | 0.514277 | 1.2:-49978881 | None:intergenic |
| GACAATACAGTCACCTTCGT+TGG | 0.516096 | 1.2:-49979200 | None:intergenic |
| TGTCAATGGTAGAGTAAAGC+TGG | 0.518786 | 1.2:+49979219 | MS.gene45037:CDS |
| AAACCATCGGAAGTATGAAT+CGG | 0.529141 | 1.2:-49978746 | None:intergenic |
| TTCGGTGCCTTCTGTCTCAA+AGG | 0.532520 | 1.2:-49978813 | None:intergenic |
| GAAGTGCAGGCCATTCCTCA+TGG | 0.533395 | 1.2:-49979243 | None:intergenic |
| AGGAAATGAGTATGTTCGTA+TGG | 0.536361 | 1.2:+49979015 | MS.gene45037:CDS |
| AAAACGTTCCTTTGAGACAA+TGG | 0.542603 | 1.2:-49978934 | None:intergenic |
| TGCCAAAATAGATTTCTCTC+CGG | 0.543347 | 1.2:+49978862 | MS.gene45037:CDS |
| GAATTGACGCTGCAATTAAG+TGG | 0.544957 | 1.2:+49978966 | MS.gene45037:CDS |
| ACCATGTTCCATTGTCTCAA+AGG | 0.547384 | 1.2:+49978926 | MS.gene45037:CDS |
| AAGCCGATTCATACTTCCGA+TGG | 0.550807 | 1.2:+49978743 | MS.gene45037:CDS |
| TTATGCTCCGGGAGCCAAGA+AGG | 0.560419 | 1.2:+49978706 | MS.gene45037:CDS |
| GAAGGTGACTGTATTGTCAA+TGG | 0.563529 | 1.2:+49979205 | MS.gene45037:CDS |
| ATCAAGAAATGGAAAACCAT+CGG | 0.570603 | 1.2:-49978759 | None:intergenic |
| GATTGTGCCTTTGAGACAGA+AGG | 0.572261 | 1.2:+49978806 | MS.gene45037:CDS |
| GCAGTTGTGAATTATGCTCC+GGG | 0.572517 | 1.2:+49978695 | MS.gene45037:CDS |
| GACGCTGCAATTAAGTGGAC+CGG | 0.578299 | 1.2:+49978971 | MS.gene45037:CDS |
| GAAATGCAGTATCAATGACT+TGG | 0.591249 | 1.2:-49978625 | None:intergenic |
| TCAGATACCGATTTACTCGA+TGG | 0.596226 | 1.2:+49978887 | MS.gene45037:CDS |
| TAAAGCTGGTCCATGAGGAA+TGG | 0.596607 | 1.2:+49979233 | MS.gene45037:CDS |
| CGTATTGATATTTCCAACGA+AGG | 0.604655 | 1.2:+49979187 | MS.gene45037:CDS |
| TCCTTTGAGACAATGGAACA+TGG | 0.636736 | 1.2:-49978927 | None:intergenic |
| TAGAGTAAAGCTGGTCCATG+AGG | 0.669372 | 1.2:+49979228 | MS.gene45037:CDS |
| ACATGGTAGTGATTGGAACA+GGG | 0.671114 | 1.2:-49978910 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATAATGTTTTCTTATTCAA+AGG | + | chr1.2:49978995-49979014 | MS.gene45037:CDS | 20.0% |
| ! | GAATAAGAAAACATTATGTC+CGG | - | chr1.2:49978993-49979012 | None:intergenic | 25.0% |
| !!! | AAAGGAACGTTTTTAGAAAA+TGG | + | chr1.2:49978944-49978963 | MS.gene45037:CDS | 25.0% |
| AATGGTGTTTCATCAAGAAA+TGG | - | chr1.2:49978773-49978792 | None:intergenic | 30.0% | |
| ATCAAGAAATGGAAAACCAT+CGG | - | chr1.2:49978762-49978781 | None:intergenic | 30.0% | |
| TGATGAAACACCATTTGAAA+AGG | + | chr1.2:49978778-49978797 | MS.gene45037:CDS | 30.0% | |
| TTTAACAACATGATCCTTCT+TGG | - | chr1.2:49978723-49978742 | None:intergenic | 30.0% | |
| ! | AATCAAGCTTACGTTTTCAA+AGG | + | chr1.2:49979151-49979170 | MS.gene45037:CDS | 30.0% |
| ! | CAATCTACTTCCTTTTCAAA+TGG | - | chr1.2:49978791-49978810 | None:intergenic | 30.0% |
| !! | TTTTAAAAGTTTGCTCGGTA+CGG | + | chr1.2:49979084-49979103 | MS.gene45037:CDS | 30.0% |
| !!! | ACTTTTAAAACCAGAACGAA+TGG | - | chr1.2:49979074-49979093 | None:intergenic | 30.0% |
| !!! | TCTGGTTTTAAAAGTTTGCT+CGG | + | chr1.2:49979079-49979098 | MS.gene45037:CDS | 30.0% |
| AGGAAATGAGTATGTTCGTA+TGG | + | chr1.2:49979015-49979034 | MS.gene45037:CDS | 35.0% | |
| AGTAAATCGGTATCTGAATC+CGG | - | chr1.2:49978884-49978903 | None:intergenic | 35.0% | |
| GAAATGCAGTATCAATGACT+TGG | - | chr1.2:49978628-49978647 | None:intergenic | 35.0% | |
| GTTACGTGTTCAAGAAGAAA+AGG | + | chr1.2:49978669-49978688 | MS.gene45037:CDS | 35.0% | |
| TGCCAAAATAGATTTCTCTC+CGG | + | chr1.2:49978862-49978881 | MS.gene45037:CDS | 35.0% | |
| ! | AAACCATCGGAAGTATGAAT+CGG | - | chr1.2:49978749-49978768 | None:intergenic | 35.0% |
| ! | ATCCGGAGAGAAATCTATTT+TGG | - | chr1.2:49978867-49978886 | None:intergenic | 35.0% |
| ! | CGTATTGATATTTCCAACGA+AGG | + | chr1.2:49979187-49979206 | MS.gene45037:CDS | 35.0% |
| !! | AAAACGTTCCTTTGAGACAA+TGG | - | chr1.2:49978937-49978956 | None:intergenic | 35.0% |
| ACCATGTTCCATTGTCTCAA+AGG | + | chr1.2:49978926-49978945 | MS.gene45037:CDS | 40.0% | |
| ACTCATTACTCCATTCGTTC+TGG | + | chr1.2:49979061-49979080 | MS.gene45037:CDS | 40.0% | |
| GAAGGTGACTGTATTGTCAA+TGG | + | chr1.2:49979205-49979224 | MS.gene45037:CDS | 40.0% | |
| GAATTGACGCTGCAATTAAG+TGG | + | chr1.2:49978966-49978985 | MS.gene45037:CDS | 40.0% | |
| TGCAGTTGTGAATTATGCTC+CGG | + | chr1.2:49978694-49978713 | MS.gene45037:CDS | 40.0% | |
| TGTCAATGGTAGAGTAAAGC+TGG | + | chr1.2:49979219-49979238 | MS.gene45037:CDS | 40.0% | |
| ! | AACATGGTAGTGATTGGAAC+AGG | - | chr1.2:49978914-49978933 | None:intergenic | 40.0% |
| ! | ACATGGTAGTGATTGGAACA+GGG | - | chr1.2:49978913-49978932 | None:intergenic | 40.0% |
| ! | CAATGGAACATGGTAGTGAT+TGG | - | chr1.2:49978920-49978939 | None:intergenic | 40.0% |
| !! | TCAGATACCGATTTACTCGA+TGG | + | chr1.2:49978887-49978906 | MS.gene45037:CDS | 40.0% |
| !! | TCCTTTGAGACAATGGAACA+TGG | - | chr1.2:49978930-49978949 | None:intergenic | 40.0% |
| !! | TTTAATAGGCTCTGAAGTGC+AGG | - | chr1.2:49979259-49979278 | None:intergenic | 40.0% |
| AACAGGGCCATCGAGTAAAT+CGG | - | chr1.2:49978897-49978916 | None:intergenic | 45.0% | |
| ACCGAAGCAATCATCTTCTC+TGG | + | chr1.2:49978830-49978849 | MS.gene45037:CDS | 45.0% | |
| GACAATACAGTCACCTTCGT+TGG | - | chr1.2:49979203-49979222 | None:intergenic | 45.0% | |
| GCAGTTGTGAATTATGCTCC+GGG | + | chr1.2:49978695-49978714 | MS.gene45037:CDS | 45.0% | |
| GCCAGAGAAGATGATTGCTT+CGG | - | chr1.2:49978834-49978853 | None:intergenic | 45.0% | |
| TAAAGCTGGTCCATGAGGAA+TGG | + | chr1.2:49979233-49979252 | MS.gene45037:CDS | 45.0% | |
| TAGAGTAAAGCTGGTCCATG+AGG | + | chr1.2:49979228-49979247 | MS.gene45037:CDS | 45.0% | |
| ! | AAGCCGATTCATACTTCCGA+TGG | + | chr1.2:49978743-49978762 | MS.gene45037:CDS | 45.0% |
| ! | CTCGGTACGGTCTTTGAAAA+TGG | + | chr1.2:49979097-49979116 | MS.gene45037:CDS | 45.0% |
| !! | GATTGTGCCTTTGAGACAGA+AGG | + | chr1.2:49978806-49978825 | MS.gene45037:CDS | 45.0% |
| ACATGATCCTTCTTGGCTCC+CGG | - | chr1.2:49978716-49978735 | None:intergenic | 50.0% | |
| GACGCTGCAATTAAGTGGAC+CGG | + | chr1.2:49978971-49978990 | MS.gene45037:CDS | 50.0% | |
| !! | TTCGGTGCCTTCTGTCTCAA+AGG | - | chr1.2:49978816-49978835 | None:intergenic | 50.0% |
| GAAGTGCAGGCCATTCCTCA+TGG | - | chr1.2:49979246-49979265 | None:intergenic | 55.0% | |
| TTATGCTCCGGGAGCCAAGA+AGG | + | chr1.2:49978706-49978725 | MS.gene45037:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 49978617 | 49979285 | 49978617 | ID=MS.gene45037 |
| chr1.2 | mRNA | 49978617 | 49979285 | 49978617 | ID=MS.gene45037.t1;Parent=MS.gene45037 |
| chr1.2 | exon | 49978617 | 49979285 | 49978617 | ID=MS.gene45037.t1.exon1;Parent=MS.gene45037.t1 |
| chr1.2 | CDS | 49978617 | 49979285 | 49978617 | ID=cds.MS.gene45037.t1;Parent=MS.gene45037.t1 |
| Gene Sequence |
| Protein sequence |
