AlfalfaGEDB | MS.gene45084

M. sativa cultivar XinJiangDaYe / MS.gene45084

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45084.t1	XP_003613295.2	89.5	38	4	0	4	41	314	351	1.60E-09	71.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45084.t1	G7K2S7	89.5	38	4	0	4	41	314	351	1.2e-09	71.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056297	MS.gene45084	0.811469	6.89E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45084.t1	MTR_5g034990	89.474	38	4	0	4	41	314	351	1.01e-16	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 13 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTGAGAGTGGAAGTGCTT+GGG	0.336529	5.3:+23953275	MS.gene45084:CDS
TCCGCCTTTAGCTTTGTTGA+TGG	0.359173	5.3:-23953308	None:intergenic
ATAAGGAAATTCTTTCTTGC+GGG	0.361526	5.3:+23953251	MS.gene45084:intron
AACAAAGCTAAAGGCGGAAT+CGG	0.447388	5.3:+23953313	MS.gene45084:CDS
GTATTGAGAGTGGAAGTGCT+TGG	0.512587	5.3:+23953274	MS.gene45084:CDS
TTGTCCATCAACAAAGCTAA+AGG	0.532127	5.3:+23953304	MS.gene45084:CDS
CATAAGGAAATTCTTTCTTG+CGG	0.536558	5.3:+23953250	MS.gene45084:intron
TTGTAACAATGGAACAACAT+TGG	0.549077	5.3:-23953027	None:intergenic
TTCTTGCGGGGTATTGAGAG+TGG	0.554327	5.3:+23953264	MS.gene45084:CDS
ATTGAGAGTGGAAGTGCTTG+GGG	0.555960	5.3:+23953276	MS.gene45084:CDS
TGAAGCTTTGCCCCATTGCA+CGG	0.560055	5.3:-23952961	None:intergenic
TAAGGAAATTCTTTCTTGCG+GGG	0.596606	5.3:+23953252	MS.gene45084:intron
TCCATCAACAAAGCTAAAGG+CGG	0.661588	5.3:+23953307	MS.gene45084:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATACTTTGTTTTGTAACAA+TGG	-	chr5.3:23953041-23953060	None:intergenic	20.0%
!	AAGAAAGAATTTCCTTATGT+TGG	-	chr5.3:23953249-23953268	None:intergenic	25.0%
!	TTTAATGAACATCATGTGAA+TGG	+	chr5.3:23953220-23953239	MS.gene45084:intron	25.0%
	AAAGCTTCAGAAGAAAAAGT+TGG	+	chr5.3:23952975-23952994	MS.gene45084:CDS	30.0%
	AAGCTTCAGAAGAAAAAGTT+GGG	+	chr5.3:23952976-23952995	MS.gene45084:CDS	30.0%
	ATAAGGAAATTCTTTCTTGC+GGG	+	chr5.3:23953251-23953270	MS.gene45084:intron	30.0%
	ATAGTTATGGGTTCTTGTTA+AGG	-	chr5.3:23953144-23953163	None:intergenic	30.0%
	CATAAGGAAATTCTTTCTTG+CGG	+	chr5.3:23953250-23953269	MS.gene45084:intron	30.0%
	TTGTAACAATGGAACAACAT+TGG	-	chr5.3:23953030-23953049	None:intergenic	30.0%
!	GGAAGGTACATATATAAAAC+TGG	+	chr5.3:23952798-23952817	MS.gene45084:intron	30.0%
	ATGACTCTTTGCAGTTTGAT+TGG	+	chr5.3:23952909-23952928	MS.gene45084:intron	35.0%
	CAGAAGAAAAAGTTGGGAAA+GGG	+	chr5.3:23952982-23953001	MS.gene45084:CDS	35.0%
	TAAGGAAATTCTTTCTTGCG+GGG	+	chr5.3:23953252-23953271	MS.gene45084:intron	35.0%
	TCAGAAGAAAAAGTTGGGAA+AGG	+	chr5.3:23952981-23953000	MS.gene45084:CDS	35.0%
	TTGTCCATCAACAAAGCTAA+AGG	+	chr5.3:23953304-23953323	MS.gene45084:CDS	35.0%
	AACAAAGCTAAAGGCGGAAT+CGG	+	chr5.3:23953313-23953332	MS.gene45084:CDS	40.0%
	TCCATCAACAAAGCTAAAGG+CGG	+	chr5.3:23953307-23953326	MS.gene45084:CDS	40.0%
	TGTGAATGGCATCCAACATA+AGG	+	chr5.3:23953234-23953253	MS.gene45084:intron	40.0%
!	AGGATTTTATGCCGTGCAAT+GGG	+	chr5.3:23952950-23952969	MS.gene45084:intron	40.0%
!	TAGGATTTTATGCCGTGCAA+TGG	+	chr5.3:23952949-23952968	MS.gene45084:intron	40.0%
!!	GATTGGTGCTGTTATAATCG+TGG	+	chr5.3:23952926-23952945	MS.gene45084:intron	40.0%
!!	TATTGAGAGTGGAAGTGCTT+GGG	+	chr5.3:23953275-23953294	MS.gene45084:CDS	40.0%
!	GGATTTTATGCCGTGCAATG+GGG	+	chr5.3:23952951-23952970	MS.gene45084:intron	45.0%
!!	ATTGAGAGTGGAAGTGCTTG+GGG	+	chr5.3:23953276-23953295	MS.gene45084:CDS	45.0%
!!	GGTGCTGTTATAATCGTGGT+AGG	+	chr5.3:23952930-23952949	MS.gene45084:intron	45.0%
!!	GTATTGAGAGTGGAAGTGCT+TGG	+	chr5.3:23953274-23953293	MS.gene45084:CDS	45.0%
!!	TCCGCCTTTAGCTTTGTTGA+TGG	-	chr5.3:23953311-23953330	None:intergenic	45.0%
!!	ATATAAATTAATAATAGTTA+TGG	-	chr5.3:23953157-23953176	None:intergenic	5.0%
!!	TATAAATTAATAATAGTTAT+GGG	-	chr5.3:23953156-23953175	None:intergenic	5.0%
	TGAAGCTTTGCCCCATTGCA+CGG	-	chr5.3:23952964-23952983	None:intergenic	50.0%
!!	TTCTTGCGGGGTATTGAGAG+TGG	+	chr5.3:23953264-23953283	MS.gene45084:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	23952784	23953351	23952784	ID=MS.gene45084
chr5.3	mRNA	23952784	23953351	23952784	ID=MS.gene45084.t1;Parent=MS.gene45084
chr5.3	exon	23952784	23952802	23952784	ID=MS.gene45084.t1.exon1;Parent=MS.gene45084.t1
chr5.3	CDS	23952784	23952802	23952784	ID=cds.MS.gene45084.t1;Parent=MS.gene45084.t1
chr5.3	exon	23952952	23953055	23952952	ID=MS.gene45084.t1.exon2;Parent=MS.gene45084.t1
chr5.3	CDS	23952952	23953055	23952952	ID=cds.MS.gene45084.t1;Parent=MS.gene45084.t1
chr5.3	exon	23953256	23953351	23953256	ID=MS.gene45084.t1.exon3;Parent=MS.gene45084.t1
chr5.3	CDS	23953256	23953351	23953256	ID=cds.MS.gene45084.t1;Parent=MS.gene45084.t1

Gene Sequence

>MS.gene45084

ATGACTTTTATCTTGGAAGGTACATATATAAAACTGGTGAATCATTTTAAGTAACATAGTTAATTACTTAATACTTGTTTATAGTGAGATTTTGTTTTGCATAATATTGAGAATAAATTTCAAAAATGACTCTTTGCAGTTTGATTGGTGCTGTTATAATCGTGGTAGGATTTTATGCCGTGCAATGGGGCAAAGCTTCAGAAGAAAAAGTTGGGAAAGGGATTCAAAACTTAGAGACACAGTCCAATGTTGTTCCATTGTTACAAAACAAAGTATAATGTTAATAACACTTTTTTTTCTTTTTGTGAAAAGTTAATAACACACTTAATTTCTAACTCATACTTTTAATTTTTCAATCCTTAACAAGAACCCATAACTATTATTAATTTATATGAGTATTACTAATTTATACTAAATGTATCTAAAATACACATGATTTAATGAACATCATGTGAATGGCATCCAACATAAGGAAATTCTTTCTTGCGGGGTATTGAGAGTGGAAGTGCTTGGGGATAAGTTGTCCATCAACAAAGCTAAAGGCGGAATCGGATTTCATAAGTCATAA

Protein sequence

>MS.gene45084.t1

MTFILEGFYAVQWGKASEEKVGKGIQNLETQSNVVPLLQNKEILSCGVLRVEVLGDKLSINKAKGGIGFHKS