AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45095

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45095.t1 MTR_5g035150 96.322 435 16 0 1 435 1 435 0.0 852
MS.gene45095.t1 MTR_5g035070 65.057 435 150 2 1 435 5 437 0.0 568
MS.gene45095.t1 MTR_5g035090 61.839 435 160 3 1 435 3 431 0.0 547
MS.gene45095.t1 MTR_5g035080 66.420 405 116 6 34 435 37 424 0.0 536
MS.gene45095.t1 MTR_3g109840 61.239 436 142 9 1 433 6 417 0.0 521
MS.gene45095.t1 MTR_8g104970 56.926 462 149 9 16 432 10 466 1.06e-180 513
MS.gene45095.t1 MTR_7g033230 61.408 355 130 4 82 433 261 611 1.40e-158 461
MS.gene45095.t1 MTR_7g033230 61.408 355 130 4 82 433 331 681 1.15e-157 461
MS.gene45095.t1 MTR_5g035140 65.283 265 85 4 172 433 7 267 1.73e-125 365
MS.gene45095.t1 MTR_8g099300 41.477 352 195 7 85 431 74 419 4.36e-93 287
MS.gene45095.t1 MTR_8g027900 40.510 353 200 5 83 431 209 555 2.40e-92 290
MS.gene45095.t1 MTR_6g016880 36.867 415 244 10 25 432 17 420 5.43e-91 282
MS.gene45095.t1 MTR_7g019120 34.383 413 249 10 29 432 22 421 7.16e-85 266
MS.gene45095.t1 MTR_6g016875 38.275 371 215 7 68 432 47 409 4.91e-83 261
MS.gene45095.t1 MTR_6g007777 37.903 372 213 8 66 433 102 459 6.12e-82 260
MS.gene45095.t1 MTR_2g016060 37.640 356 197 12 90 433 60 402 9.89e-76 242
MS.gene45095.t1 MTR_1g076690 33.609 363 214 11 85 433 223 572 2.91e-64 217
MS.gene45095.t1 MTR_6g061710 32.447 376 205 13 83 433 165 516 4.79e-63 212
MS.gene45095.t1 MTR_7g094750 32.880 368 206 14 84 433 90 434 4.09e-52 181
MS.gene45095.t1 MTR_2g022560 33.693 371 202 14 84 435 96 441 1.68e-49 174
MS.gene45095.t1 MTR_5g035120 50.588 170 73 3 4 173 5 163 1.37e-47 161
MS.gene45095.t1 MTR_0136s0010 32.787 366 206 14 85 433 170 512 8.56e-46 166
MS.gene45095.t1 MTR_4g126950 32.353 374 194 16 83 431 107 446 1.90e-45 164
MS.gene45095.t1 MTR_7g093450 30.935 417 234 19 43 433 202 590 4.48e-45 165
MS.gene45095.t1 MTR_1g058380 30.108 372 212 15 83 433 53 397 5.24e-43 156
MS.gene45095.t1 MTR_4g125060 30.518 367 217 12 76 427 124 467 1.45e-42 156
MS.gene45095.t1 MTR_7g109550 31.006 358 201 14 85 427 137 463 1.73e-42 156
MS.gene45095.t1 MTR_4g125060 30.518 367 217 12 76 427 192 535 2.90e-42 157
MS.gene45095.t1 MTR_4g125060 30.518 367 217 12 76 427 163 506 3.14e-42 156
MS.gene45095.t1 MTR_5g059400 30.939 362 206 15 84 426 174 510 4.60e-42 156
MS.gene45095.t1 MTR_1g116130 30.939 362 206 15 84 426 174 510 4.60e-42 156
MS.gene45095.t1 MTR_4g016940 30.053 376 213 16 79 431 79 427 4.98e-42 154
MS.gene45095.t1 MTR_8g093530 30.532 357 212 12 83 431 88 416 3.19e-41 152
MS.gene45095.t1 MTR_5g071350 30.110 362 226 10 77 427 50 395 4.34e-41 150
MS.gene45095.t1 MTR_8g069790 29.815 379 221 15 73 432 166 518 5.06e-41 153
MS.gene45095.t1 MTR_4g121560 28.302 424 251 18 27 431 64 453 5.97e-41 152
MS.gene45095.t1 MTR_4g014440 30.278 360 216 15 85 427 62 403 2.55e-40 149
MS.gene45095.t1 MTR_7g068920 31.347 386 213 16 69 431 65 421 7.54e-40 148
MS.gene45095.t1 MTR_8g463790 26.824 425 260 15 23 427 12 405 1.10e-39 147
MS.gene45095.t1 MTR_5g033800 28.142 366 229 12 81 433 141 485 2.50e-39 148
MS.gene45095.t1 MTR_3g035830 29.885 348 209 11 83 427 29 344 8.60e-39 143
MS.gene45095.t1 MTR_3g464570 29.799 349 226 11 82 427 40 372 1.17e-38 144
MS.gene45095.t1 MTR_7g070060 30.194 361 215 15 85 427 64 405 1.81e-38 144
MS.gene45095.t1 MTR_2g073510 31.268 355 208 13 82 431 30 353 7.29e-38 141
MS.gene45095.t1 MTR_3g090120 30.155 388 210 14 69 433 113 462 3.25e-37 141
MS.gene45095.t1 MTR_6g016240 28.160 451 259 18 8 431 4 416 6.35e-37 140
MS.gene45095.t1 MTR_3g021090 30.028 353 202 14 83 427 29 344 3.11e-36 138
MS.gene45095.t1 MTR_3g070900 28.611 360 226 13 82 427 103 445 4.10e-36 138
MS.gene45095.t1 MTR_8g093870 26.722 363 231 9 83 431 131 472 1.99e-35 137
MS.gene45095.t1 MTR_7g094750 33.992 253 138 9 84 321 90 328 5.62e-35 132
MS.gene45095.t1 MTR_3g021060 29.513 349 209 12 83 427 44 359 1.97e-34 132
MS.gene45095.t1 MTR_3g049330 30.387 362 206 14 76 427 21 346 4.84e-34 130
MS.gene45095.t1 MTR_4g088030 30.029 343 207 10 84 424 32 343 6.04e-34 130
MS.gene45095.t1 MTR_2g073500 29.888 358 205 14 82 431 26 345 6.06e-34 130
MS.gene45095.t1 MTR_5g083260 29.006 362 220 12 82 427 80 420 1.73e-33 130
MS.gene45095.t1 MTR_2g091175 30.347 346 205 11 85 427 50 362 1.94e-33 129
MS.gene45095.t1 MTR_4g087730 30.321 343 206 10 84 424 44 355 2.35e-33 129
MS.gene45095.t1 MTR_1g050448 27.838 370 231 12 67 431 37 375 2.49e-33 129
MS.gene45095.t1 MTR_4g092830 27.700 426 239 17 41 431 1 392 1.52e-32 128
MS.gene45095.t1 MTR_3g071650 27.778 360 221 11 85 427 89 426 1.67e-32 128
MS.gene45095.t1 MTR_1g112210 26.741 359 218 13 80 427 54 378 3.36e-32 126
MS.gene45095.t1 MTR_4g079700 28.609 381 236 13 55 431 15 363 8.96e-32 124
MS.gene45095.t1 MTR_2g015720 29.508 366 218 14 70 431 29 358 1.54e-31 124
MS.gene45095.t1 MTR_5g034810 29.630 351 206 13 83 427 33 348 2.14e-29 118
MS.gene45095.t1 MTR_1g112210 25.843 356 212 12 80 427 54 365 2.77e-29 117
MS.gene45095.t1 MTR_5g082880 26.593 361 233 11 83 427 163 507 2.83e-29 120
MS.gene45095.t1 MTR_4g014430 27.500 400 243 15 51 431 75 446 5.85e-29 118
MS.gene45095.t1 MTR_1g111010 28.142 366 212 14 73 431 20 341 1.48e-28 115
MS.gene45095.t1 MTR_7g019140 36.667 150 86 3 29 173 22 167 2.00e-28 110
MS.gene45095.t1 MTR_5g033800 31.667 180 116 3 81 254 141 319 1.47e-26 110
MS.gene45095.t1 MTR_7g070060 30.488 246 146 9 85 319 64 295 6.21e-23 99.0
MS.gene45095.t1 MTR_7g070060 28.617 311 180 15 137 427 15 303 1.56e-22 97.8
MS.gene45095.t1 MTR_3g464570 28.014 282 184 11 149 427 3 268 6.16e-22 95.5
MS.gene45095.t1 MTR_4g102570 39.370 127 65 3 201 321 2 122 6.35e-22 92.0
MS.gene45095.t1 MTR_7g117160 31.633 196 81 6 235 430 47 189 8.72e-19 84.3
MS.gene45095.t1 MTR_7g019130 30.400 125 80 3 206 326 4 125 6.45e-13 66.2
MS.gene45095.t1 MTR_0929s0010 30.909 110 67 3 29 133 22 127 1.66e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45095.t1 AT1G70230 48.585 424 186 8 27 435 9 415 6.51e-149 430
MS.gene45095.t1 AT5G15890 39.276 387 216 8 50 432 152 523 1.79e-97 302
MS.gene45095.t1 AT3G02440 39.326 356 195 7 85 432 127 469 1.44e-90 283
MS.gene45095.t1 AT3G02440 39.326 356 195 7 85 432 135 477 1.80e-90 283
MS.gene45095.t1 AT4G23790 36.916 428 257 9 10 431 7 427 1.31e-89 278
MS.gene45095.t1 AT4G01080 38.596 399 220 9 35 429 51 428 2.02e-89 278
MS.gene45095.t1 AT1G01430 38.360 378 218 8 61 431 78 447 3.69e-88 276
MS.gene45095.t1 AT3G28150 36.543 405 239 10 36 433 19 412 1.17e-87 273
MS.gene45095.t1 AT4G11090 36.574 432 253 12 9 431 6 425 6.57e-87 272
MS.gene45095.t1 AT5G15900 36.675 379 222 10 65 435 45 413 9.79e-81 256
MS.gene45095.t1 AT5G15900 36.675 379 222 10 65 435 64 432 1.39e-80 256
MS.gene45095.t1 AT4G25360 34.005 397 233 11 51 433 145 526 5.83e-70 231
MS.gene45095.t1 AT4G25360 34.005 397 233 11 51 433 145 526 5.83e-70 231
MS.gene45095.t1 AT5G51640 34.091 396 216 14 63 433 115 490 2.96e-66 220
MS.gene45095.t1 AT5G19160 35.519 366 207 11 84 435 98 448 8.70e-57 194
MS.gene45095.t1 AT3G06080 32.231 363 218 10 85 435 103 449 3.98e-52 182
MS.gene45095.t1 AT3G12060 31.915 376 212 16 77 433 188 538 1.10e-49 177
MS.gene45095.t1 AT5G06700 31.868 364 216 15 83 433 253 597 5.50e-46 168
MS.gene45095.t1 AT1G48880 34.043 376 196 17 71 428 95 436 4.25e-45 162
MS.gene45095.t1 AT5G64020 30.811 370 215 14 85 431 57 408 8.60e-45 161
MS.gene45095.t1 AT5G20590 31.522 368 214 14 75 427 131 475 3.83e-43 158
MS.gene45095.t1 AT3G11570 29.954 434 238 17 20 431 30 419 1.62e-42 155
MS.gene45095.t1 AT2G14530 29.286 420 236 19 36 431 24 406 9.46e-42 153
MS.gene45095.t1 AT3G62390 29.086 361 224 13 83 433 136 474 2.33e-41 153
MS.gene45095.t1 AT5G58600 29.570 372 228 11 68 431 55 400 2.83e-41 151
MS.gene45095.t1 AT5G49340 29.640 361 215 12 84 427 99 437 5.85e-40 149
MS.gene45095.t1 AT2G30010 31.707 369 204 11 84 431 55 396 1.01e-39 147
MS.gene45095.t1 AT3G14850 32.312 359 205 11 79 431 28 354 1.63e-39 145
MS.gene45095.t1 AT2G30010 32.162 370 201 13 84 431 98 439 1.87e-39 147
MS.gene45095.t1 AT2G31110 29.722 360 217 13 72 427 31 358 9.55e-39 144
MS.gene45095.t1 AT2G38320 28.453 362 230 12 80 427 54 400 2.26e-38 144
MS.gene45095.t1 AT5G06230 30.214 374 208 15 83 431 19 364 2.33e-38 142
MS.gene45095.t1 AT5G06230 30.749 374 206 16 83 431 60 405 4.14e-38 143
MS.gene45095.t1 AT5G19160 36.992 246 138 8 84 320 98 335 1.22e-36 137
MS.gene45095.t1 AT5G01360 28.809 361 223 13 83 428 34 375 1.84e-36 137
MS.gene45095.t1 AT1G73140 29.759 373 218 17 72 427 56 401 2.46e-36 138
MS.gene45095.t1 AT5G01360 28.809 361 223 13 83 428 90 431 6.89e-36 137
MS.gene45095.t1 AT2G42570 29.915 351 209 13 85 431 47 364 7.76e-36 135
MS.gene45095.t1 AT1G60790 28.032 371 222 15 83 432 188 534 1.45e-35 138
MS.gene45095.t1 AT5G20680 26.952 397 246 12 57 431 83 457 2.05e-35 136
MS.gene45095.t1 AT2G37720 28.804 368 217 12 83 431 141 482 3.03e-35 136
MS.gene45095.t1 AT5G20680 26.952 397 246 12 57 431 159 533 5.76e-35 136
MS.gene45095.t1 AT5G20680 26.952 397 246 12 57 431 177 551 7.16e-35 136
MS.gene45095.t1 AT5G20680 26.952 397 246 12 57 431 177 551 7.16e-35 136
MS.gene45095.t1 AT5G20680 26.952 397 246 12 57 431 177 551 7.16e-35 136
MS.gene45095.t1 AT5G20680 26.952 397 246 12 57 431 177 551 7.16e-35 136
MS.gene45095.t1 AT5G64470 28.333 360 225 14 85 431 55 394 1.10e-34 133
MS.gene45095.t1 AT3G11030 27.374 358 230 11 83 427 104 444 1.12e-34 134
MS.gene45095.t1 AT2G38320 28.834 326 204 11 115 427 7 317 1.94e-34 131
MS.gene45095.t1 AT2G30900 28.977 352 214 11 82 427 40 361 3.58e-34 131
MS.gene45095.t1 AT3G55990 28.532 361 223 13 83 427 138 479 7.59e-34 132
MS.gene45095.t1 AT2G40160 27.123 365 222 14 85 427 77 419 1.04e-33 131
MS.gene45095.t1 AT2G40160 27.123 365 222 14 85 427 77 419 1.26e-33 131
MS.gene45095.t1 AT2G40320 27.869 366 219 13 82 427 78 418 2.15e-33 130
MS.gene45095.t1 AT5G64470 28.142 366 224 15 85 431 55 400 3.72e-33 129
MS.gene45095.t1 AT2G34070 27.841 352 218 12 84 431 64 383 4.28e-33 129
MS.gene45095.t1 AT1G29050 28.532 361 221 13 75 431 51 378 4.70e-33 128
MS.gene45095.t1 AT3G54260 28.056 360 210 14 85 431 51 374 5.68e-32 125
MS.gene45095.t1 AT2G40150 28.165 387 232 16 63 427 54 416 9.36e-32 125
MS.gene45095.t1 AT3G06080 31.984 247 149 6 85 321 103 340 1.25e-31 124
MS.gene45095.t1 AT1G78710 29.558 362 215 15 76 431 29 356 2.95e-29 117
MS.gene45095.t1 AT3G62390 29.268 246 161 6 83 322 136 374 5.06e-29 118
MS.gene45095.t1 AT5G20590 31.418 261 152 10 75 321 131 378 1.02e-27 114
MS.gene45095.t1 AT5G01620 26.133 375 230 13 77 431 102 449 1.05e-27 114
MS.gene45095.t1 AT5G01620 26.133 375 230 13 77 431 102 449 1.05e-27 114
MS.gene45095.t1 AT5G01620 26.133 375 230 13 77 431 110 457 1.14e-27 114
MS.gene45095.t1 AT5G58600 31.621 253 154 8 68 319 55 289 7.72e-27 109
MS.gene45095.t1 AT2G31110 33.152 184 115 4 72 254 31 207 2.22e-24 102
MS.gene45095.t1 AT5G06230 30.916 262 142 11 83 321 60 305 1.67e-23 100
MS.gene45095.t1 AT2G40160 36.641 131 81 2 85 214 77 206 3.15e-23 99.8
MS.gene45095.t1 AT2G30900 33.143 175 110 3 82 255 40 208 4.24e-21 93.2
MS.gene45095.t1 AT5G64470 28.030 264 168 11 85 335 55 309 9.52e-19 87.0
MS.gene45095.t1 AT5G64470 28.030 264 168 11 85 335 55 309 1.17e-18 87.0
MS.gene45095.t1 AT3G14850 29.787 282 160 11 156 431 2 251 2.88e-18 84.3
MS.gene45095.t1 AT1G78710 31.111 180 115 4 76 254 22 193 5.34e-17 81.6
MS.gene45095.t1 AT2G34070 26.449 276 167 12 156 427 2 245 1.97e-15 76.3

Find 96 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCCACTTACGAAATTTAT+TGG 0.120096 5.3:+24049998 None:intergenic
CTGATTTAGGTTATCTTTAT+TGG 0.135262 5.3:-24050199 MS.gene45095:CDS
TTCTCTCAATTATACTATTT+AGG 0.139860 5.3:+24049682 None:intergenic
GTCCTCAAAGCCTTTCTTAA+TGG 0.190045 5.3:+24049708 None:intergenic
TATGAGAGTGATTTAGTTCT+TGG 0.213205 5.3:-24049780 MS.gene45095:CDS
TCTCTCAATTATACTATTTA+GGG 0.267875 5.3:+24049683 None:intergenic
TGCAAAGAAGTGACTCTAAT+TGG 0.285207 5.3:+24050069 None:intergenic
ACAAATGGTAGACCTGATTT+AGG 0.287971 5.3:-24050212 MS.gene45095:CDS
TCAACACCATGCACTAGAAA+TGG 0.356439 5.3:+24049936 None:intergenic
GAATGGATGGTGAGATTAGA+AGG 0.357080 5.3:-24049527 MS.gene45095:CDS
AGAAAAGTTGCTAAAGGAAA+TGG 0.366030 5.3:-24049663 MS.gene45095:CDS
TATAAACAAAGAAGGGATAT+AGG 0.366305 5.3:+24050659 None:intergenic
GTGACTATTCAAATGGAGAT+TGG 0.369081 5.3:-24050307 MS.gene45095:CDS
GGTGTTGGATGTAACTAAAT+TGG 0.388499 5.3:-24049439 MS.gene45095:CDS
GACATGATTGTTATATCAAC+CGG 0.390990 5.3:-24049831 MS.gene45095:CDS
TACAAAATGATTGCGTTCAT+TGG 0.394768 5.3:-24049341 MS.gene45095:CDS
ATGTGGTGACATTGTATATA+GGG 0.397785 5.3:+24050267 None:intergenic
AGAAAAGAGGATAAGATAGT+TGG 0.399914 5.3:-24049552 MS.gene45095:CDS
ATTTATTGGACTTGCCATCT+TGG 0.403569 5.3:+24050012 None:intergenic
CAACACCATGCACTAGAAAT+GGG 0.415330 5.3:+24049937 None:intergenic
CCATCTTGGTAAACAAGGTT+AGG 0.420926 5.3:+24050026 None:intergenic
TGCGTTCATTGGTGCTTGCC+AGG 0.424514 5.3:-24049330 MS.gene45095:CDS
TTCCATTAAGAAAGGCTTTG+AGG 0.431515 5.3:-24049710 MS.gene45095:CDS
TTTGGAACTCCATTTGCAAA+TGG 0.431908 5.3:+24049369 None:intergenic
TGAATAGTGGTAGGTGGAAA+TGG 0.434406 5.3:+24050580 None:intergenic
TCACTCTCATAGTAAATTGA+AGG 0.436067 5.3:+24049792 None:intergenic
GGATAAGATAGTTGGAGGAA+TGG 0.436305 5.3:-24049544 MS.gene45095:CDS
ACTCCATTTGCAAATGGAAA+AGG 0.436668 5.3:+24049375 None:intergenic
GGCCAAGGATATTGATCAAA+TGG 0.439901 5.3:-24049853 MS.gene45095:CDS
AAGAGTAAGTAGATGAATAG+TGG 0.440562 5.3:+24050567 None:intergenic
CATGTGGTGACATTGTATAT+AGG 0.444034 5.3:+24050266 None:intergenic
CAATAAAGATAACCTAAATC+AGG 0.445212 5.3:+24050200 None:intergenic
GTTTGTTCTTAATGAGATTG+AGG 0.450023 5.3:+24050126 None:intergenic
CTTGGTAGATTACACTCACT+TGG 0.451639 5.3:+24050167 None:intergenic
TGACTATTCAAATGGAGATT+GGG 0.453167 5.3:-24050306 MS.gene45095:CDS
TAGGTTATCTTTATTGGAGA+TGG 0.460710 5.3:-24050193 MS.gene45095:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5.3:+24049405 None:intergenic
AGCAGCTTAGTTGGTGCAAA+AGG 0.466236 5.3:+24050614 None:intergenic
TAGTTGGTGCAAAAGGAGTA+AGG 0.469996 5.3:+24050621 None:intergenic
AAAGAGGATAAGATAGTTGG+AGG 0.471238 5.3:-24049549 MS.gene45095:CDS
AAATCATGTGACTATTCAAA+TGG 0.471379 5.3:-24050314 MS.gene45095:CDS
TTGAGACCAGATGGTCATCC+AGG 0.473377 5.3:-24049411 MS.gene45095:CDS
TGTAGAGATGATGAAGAAGT+GGG 0.476890 5.3:-24049280 MS.gene45095:CDS
TGGTCCCCATTTCTAGTGCA+TGG 0.482297 5.3:-24049942 MS.gene45095:CDS
TGATGAAGTATAAACAAAGA+AGG 0.490126 5.3:+24050651 None:intergenic
GACTAAGCCTTATAGAAAAG+AGG 0.491810 5.3:-24049565 MS.gene45095:CDS
GTAAAAGCTAATAAATTTGA+AGG 0.492014 5.3:-24049474 MS.gene45095:CDS
GTCCATTTGATCAATATCCT+TGG 0.493677 5.3:+24049851 None:intergenic
CCTAACCTTGTTTACCAAGA+TGG 0.495701 5.3:-24050026 MS.gene45095:CDS
AATGGAAAAGGATTCATGTA+AGG 0.496598 5.3:+24049387 None:intergenic
AAGATAGTTGGAGGAATGGA+TGG 0.499482 5.3:-24049540 MS.gene45095:CDS
GTAGAATGAAGCAGCTTAGT+TGG 0.499755 5.3:+24050605 None:intergenic
TTGTAGAGATGATGAAGAAG+TGG 0.501696 5.3:-24049281 MS.gene45095:CDS
TGCCAGGACCTATAGATACT+TGG 0.502047 5.3:-24049314 MS.gene45095:CDS
GGAGGAAAGATTGTCATTGA+AGG 0.503097 5.3:+24050724 None:intergenic
GTAAGTAGATGAATAGTGGT+AGG 0.506588 5.3:+24050571 None:intergenic
TGGATCTTGTCAATGAGAGG+TGG 0.507379 5.3:-24049875 MS.gene45095:CDS
AGTGAGAGATGCATCACAAA+TGG 0.510170 5.3:-24050227 MS.gene45095:CDS
TTGTTGGGGATTCTTTGGCT+AGG 0.510878 5.3:-24050094 MS.gene45095:CDS
ATTGAGAGAAAAGTTGCTAA+AGG 0.514738 5.3:-24049669 MS.gene45095:CDS
AAAGTGCTTGGTTCAAACCT+TGG 0.519498 5.3:+24050149 None:intergenic
GAAGGTGATTGGGATAAGGC+TGG 0.521359 5.3:-24049600 MS.gene45095:CDS
GGTGTTGAAAGATCAAATGA+AGG 0.522081 5.3:-24049921 MS.gene45095:CDS
ATCTCATTCCAAGTATCTAT+AGG 0.524335 5.3:+24049306 None:intergenic
GAGGAAAGATTGTCATTGAA+GGG 0.526115 5.3:+24050725 None:intergenic
AAGGATTGTGATAGATGAAG+TGG 0.526251 5.3:-24049508 MS.gene45095:CDS
TGGGGACCAATACAAAGAAA+AGG 0.526383 5.3:+24049956 None:intergenic
GATGAAGTATAAACAAAGAA+GGG 0.527867 5.3:+24050652 None:intergenic
AGTCCAATAAATTTCGTAAG+TGG 0.528019 5.3:-24050001 MS.gene45095:CDS
TTGTTATATCAACCGGGCAT+TGG 0.533327 5.3:-24049824 MS.gene45095:CDS
TTCCAAGTATCTATAGGTCC+TGG 0.536833 5.3:+24049312 None:intergenic
ATACCTACAAAAGAAGTTGA+AGG 0.538773 5.3:+24050539 None:intergenic
ATTTGAAGGTATTAGACTTG+AGG 0.544151 5.3:-24049460 MS.gene45095:CDS
GGATCTTGTCAATGAGAGGT+GGG 0.546338 5.3:-24049874 MS.gene45095:CDS
AGGTATTAGACTTGAGGTGT+TGG 0.547694 5.3:-24049454 MS.gene45095:CDS
CAATCACCTTCAAAATGAGC+AGG 0.550833 5.3:+24049612 None:intergenic
ATGAGATTGAGGAAAGTGCT+TGG 0.551052 5.3:+24050137 None:intergenic
TTCTTTGATTGTGCCACATG+TGG 0.561553 5.3:+24050250 None:intergenic
AGGAAAGATTGTCATTGAAG+GGG 0.571923 5.3:+24050726 None:intergenic
AGTAGATGAATAGTGGTAGG+TGG 0.577718 5.3:+24050574 None:intergenic
AATTTGAGTATGATTAAACT+CGG 0.579926 5.3:+24049743 None:intergenic
TGTCAATGAGAGGTGGGCCA+AGG 0.581180 5.3:-24049868 MS.gene45095:CDS
ACTTGCCATCTTGGTAAACA+AGG 0.587524 5.3:+24050021 None:intergenic
TTGGCATTATTGAGACCAGA+TGG 0.590154 5.3:-24049420 MS.gene45095:CDS
AAAGGATTCATGTAAGGACC+TGG 0.594011 5.3:+24049393 None:intergenic
AGATGAAGTGGAAAGTGCCA+AGG 0.625181 5.3:-24049496 MS.gene45095:CDS
ATATACAATGTCACCACATG+TGG 0.627135 5.3:-24050263 MS.gene45095:CDS
ATTTGAGTATGATTAAACTC+GGG 0.631629 5.3:+24049744 None:intergenic
GTGTTGAAAGATCAAATGAA+GGG 0.634222 5.3:-24049920 MS.gene45095:CDS
AGTTGGTGCAAAAGGAGTAA+GGG 0.640399 5.3:+24050622 None:intergenic
CAAGTGAGTGTAATCTACCA+AGG 0.650297 5.3:-24050166 MS.gene45095:CDS
CCTACAAAAGAAGTTGAAGG+AGG 0.659746 5.3:+24050542 None:intergenic
AACACCATGCACTAGAAATG+GGG 0.662494 5.3:+24049938 None:intergenic
ACATGATTGTTATATCAACC+GGG 0.662677 5.3:-24049830 MS.gene45095:CDS
GATTGGGTTAGTGACATGAG+AGG 0.690038 5.3:-24050290 MS.gene45095:CDS
ATAGTGGTAGGTGGAAATGG+TGG 0.690039 5.3:+24050583 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAATACAATGTTTT+TGG - chr5.3:24050120-24050139 MS.gene45095:CDS 10.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5.3:24049315-24049334 None:intergenic 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5.3:24049654-24049673 MS.gene45095:CDS 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5.3:24049653-24049672 MS.gene45095:CDS 15.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5.3:24049312-24049331 None:intergenic 20.0%
!! AATTTGAGTATGATTAAACT+CGG + chr5.3:24050275-24050294 None:intergenic 20.0%
!! GTAAAAGCTAATAAATTTGA+AGG - chr5.3:24050541-24050560 MS.gene45095:intron 20.0%
!! TCTCTCAATTATACTATTTA+GGG + chr5.3:24050335-24050354 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTT+AGG + chr5.3:24050336-24050355 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTACCT+TGG + chr5.3:24050539-24050558 None:intergenic 20.0%
!!! CAATCATTTTGTACATATTT+TGG + chr5.3:24050667-24050686 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG - chr5.3:24050297-24050316 MS.gene45095:CDS 20.0%
! AAATCATGTGACTATTCAAA+TGG - chr5.3:24049701-24049720 MS.gene45095:CDS 25.0%
! ATTTGAGTATGATTAAACTC+GGG + chr5.3:24050274-24050293 None:intergenic 25.0%
! CAATAAAGATAACCTAAATC+AGG + chr5.3:24049818-24049837 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5.3:24049366-24049385 None:intergenic 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5.3:24049359-24049378 None:intergenic 25.0%
! TGATGAAGTATAAACAAAGA+AGG + chr5.3:24049367-24049386 None:intergenic 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5.3:24049905-24049924 MS.gene45095:CDS 25.0%
!! ACAAACATATAGCTTTTGTT+GGG - chr5.3:24049906-24049925 MS.gene45095:CDS 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG + chr5.3:24050460-24050479 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5.3:24049816-24049835 MS.gene45095:CDS 25.0%
!!! CTAGCAATTTGTTACTATTA+AGG - chr5.3:24049610-24049629 MS.gene45095:CDS 25.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5.3:24049451-24049470 None:intergenic 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5.3:24050631-24050650 None:intergenic 30.0%
ACATGATTGTTATATCAACC+GGG - chr5.3:24050185-24050204 MS.gene45095:CDS 30.0%
AGAAAAGAGGATAAGATAGT+TGG - chr5.3:24050463-24050482 MS.gene45095:intron 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5.3:24050352-24050371 MS.gene45095:intron 30.0%
AGTCCAATAAATTTCGTAAG+TGG - chr5.3:24050014-24050033 MS.gene45095:CDS 30.0%
ATACCTACAAAAGAAGTTGA+AGG + chr5.3:24049479-24049498 None:intergenic 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5.3:24050712-24050731 None:intergenic 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5.3:24049751-24049770 None:intergenic 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5.3:24050346-24050365 MS.gene45095:intron 30.0%
GACATGATTGTTATATCAAC+CGG - chr5.3:24050184-24050203 MS.gene45095:CDS 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5.3:24050095-24050114 MS.gene45095:CDS 30.0%
GTTTGTTCTTAATGAGATTG+AGG + chr5.3:24049892-24049911 None:intergenic 30.0%
TACAAAATGATTGCGTTCAT+TGG - chr5.3:24050674-24050693 MS.gene45095:CDS 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5.3:24049822-24049841 MS.gene45095:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5.3:24050226-24050245 None:intergenic 30.0%
TGACTATTCAAATGGAGATT+GGG - chr5.3:24049709-24049728 MS.gene45095:CDS 30.0%
TGTTACTATTAAGGACTTCT+TGG - chr5.3:24049619-24049638 MS.gene45095:CDS 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5.3:24050637-24050656 MS.gene45095:CDS 30.0%
! ATGCTACCTTTTCTTTGTAT+TGG - chr5.3:24050053-24050072 MS.gene45095:CDS 30.0%
! CAAACATATAGCTTTTGTTG+GGG - chr5.3:24049907-24049926 MS.gene45095:CDS 30.0%
!! ATTTGAAGGTATTAGACTTG+AGG - chr5.3:24050555-24050574 MS.gene45095:CDS 30.0%
!! TATGAGAGTGATTTAGTTCT+TGG - chr5.3:24050235-24050254 MS.gene45095:CDS 30.0%
AAAGAGGATAAGATAGTTGG+AGG - chr5.3:24050466-24050485 MS.gene45095:intron 35.0%
AAGGATTGTGATAGATGAAG+TGG - chr5.3:24050507-24050526 MS.gene45095:intron 35.0%
ACAAATGGTAGACCTGATTT+AGG - chr5.3:24049803-24049822 MS.gene45095:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5.3:24050643-24050662 None:intergenic 35.0%
AGGAAAGATTGTCATTGAAG+GGG + chr5.3:24049292-24049311 None:intergenic 35.0%
ATATACAATGTCACCACATG+TGG - chr5.3:24049752-24049771 MS.gene45095:CDS 35.0%
ATTTATTGGACTTGCCATCT+TGG + chr5.3:24050006-24050025 None:intergenic 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5.3:24049752-24049771 None:intergenic 35.0%
GAGGAAAGATTGTCATTGAA+GGG + chr5.3:24049293-24049312 None:intergenic 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5.3:24049447-24049466 None:intergenic 35.0%
GTCCATTTGATCAATATCCT+TGG + chr5.3:24050167-24050186 None:intergenic 35.0%
GTGACTATTCAAATGGAGAT+TGG - chr5.3:24049708-24049727 MS.gene45095:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5.3:24049949-24049968 None:intergenic 35.0%
TGTAGAGATGATGAAGAAGT+GGG - chr5.3:24050735-24050754 MS.gene45095:CDS 35.0%
TTCCATTAAGAAAGGCTTTG+AGG - chr5.3:24050305-24050324 MS.gene45095:CDS 35.0%
TTGTAGAGATGATGAAGAAG+TGG - chr5.3:24050734-24050753 MS.gene45095:CDS 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5.3:24050450-24050469 MS.gene45095:intron 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5.3:24050094-24050113 MS.gene45095:CDS 35.0%
! GGTGTTGGATGTAACTAAAT+TGG - chr5.3:24050576-24050595 MS.gene45095:CDS 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5.3:24050649-24050668 None:intergenic 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5.3:24050397-24050416 MS.gene45095:intron 35.0%
! TTTTGGATCTTGTCAATGAG+AGG - chr5.3:24050137-24050156 MS.gene45095:CDS 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5.3:24050020-24050039 None:intergenic 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5.3:24050405-24050424 MS.gene45095:intron 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5.3:24050411-24050430 MS.gene45095:intron 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG - chr5.3:24049664-24049683 MS.gene45095:CDS 35.0%
AAAGGATTCATGTAAGGACC+TGG + chr5.3:24050625-24050644 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5.3:24050080-24050099 None:intergenic 40.0%
AAGATAGTTGGAGGAATGGA+TGG - chr5.3:24050475-24050494 MS.gene45095:intron 40.0%
ACTTGCCATCTTGGTAAACA+AGG + chr5.3:24049997-24050016 None:intergenic 40.0%
AGGTATTAGACTTGAGGTGT+TGG - chr5.3:24050561-24050580 MS.gene45095:CDS 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5.3:24049444-24049463 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5.3:24049788-24049807 MS.gene45095:CDS 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5.3:24050081-24050100 None:intergenic 40.0%
CAAGTGAGTGTAATCTACCA+AGG - chr5.3:24049849-24049868 MS.gene45095:CDS 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5.3:24050406-24050425 None:intergenic 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5.3:24049992-24050011 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5.3:24049476-24049495 None:intergenic 40.0%
CTTGGTAGATTACACTCACT+TGG + chr5.3:24049851-24049870 None:intergenic 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5.3:24050488-24050507 MS.gene45095:intron 40.0%
GGAGGAAAGATTGTCATTGA+AGG + chr5.3:24049294-24049313 None:intergenic 40.0%
GGATAAGATAGTTGGAGGAA+TGG - chr5.3:24050471-24050490 MS.gene45095:intron 40.0%
GGCCAAGGATATTGATCAAA+TGG - chr5.3:24050162-24050181 MS.gene45095:CDS 40.0%
GTAGAATGAAGCAGCTTAGT+TGG + chr5.3:24049413-24049432 None:intergenic 40.0%
GTCCTCAAAGCCTTTCTTAA+TGG + chr5.3:24050310-24050329 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5.3:24050082-24050101 None:intergenic 40.0%
TGAATAGTGGTAGGTGGAAA+TGG + chr5.3:24049438-24049457 None:intergenic 40.0%
TGGGGACCAATACAAAGAAA+AGG + chr5.3:24050062-24050081 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5.3:24050706-24050725 None:intergenic 40.0%
TTCTTTGATTGTGCCACATG+TGG + chr5.3:24049768-24049787 None:intergenic 40.0%
TTGGCATTATTGAGACCAGA+TGG - chr5.3:24050595-24050614 MS.gene45095:CDS 40.0%
TTGTTATATCAACCGGGCAT+TGG - chr5.3:24050191-24050210 MS.gene45095:CDS 40.0%
! AAAGTGCTTGGTTCAAACCT+TGG + chr5.3:24049869-24049888 None:intergenic 40.0%
! AGCTTTTGTTGGGGATTCTT+TGG - chr5.3:24049916-24049935 MS.gene45095:CDS 40.0%
! AGTTGGTGCAAAAGGAGTAA+GGG + chr5.3:24049396-24049415 None:intergenic 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5.3:24049989-24050008 MS.gene45095:CDS 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5.3:24049473-24049492 MS.gene45095:CDS 40.0%
! TAGTTGGTGCAAAAGGAGTA+AGG + chr5.3:24049397-24049416 None:intergenic 40.0%
!! ATGAGATTGAGGAAAGTGCT+TGG + chr5.3:24049881-24049900 None:intergenic 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5.3:24050404-24050423 MS.gene45095:intron 40.0%
AGGAAGCAAAAACCAATGCC+CGG + chr5.3:24050206-24050225 None:intergenic 45.0%
ATAGTGGTAGGTGGAAATGG+TGG + chr5.3:24049435-24049454 None:intergenic 45.0%
GGATCTTGTCAATGAGAGGT+GGG - chr5.3:24050141-24050160 MS.gene45095:CDS 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5.3:24050701-24050720 MS.gene45095:CDS 45.0%
TGGATCTTGTCAATGAGAGG+TGG - chr5.3:24050140-24050159 MS.gene45095:CDS 45.0%
TTGTTGGGGATTCTTTGGCT+AGG - chr5.3:24049921-24049940 MS.gene45095:CDS 45.0%
! AGCAGCTTAGTTGGTGCAAA+AGG + chr5.3:24049404-24049423 None:intergenic 45.0%
!! AGATGAAGTGGAAAGTGCCA+AGG - chr5.3:24050519-24050538 MS.gene45095:intron 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5.3:24049725-24049744 MS.gene45095:CDS 45.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5.3:24050415-24050434 MS.gene45095:intron 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5.3:24050613-24050632 None:intergenic 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5.3:24050604-24050623 MS.gene45095:CDS 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5.3:24050073-24050092 MS.gene45095:CDS 50.0%
TGTCAATGAGAGGTGGGCCA+AGG - chr5.3:24050147-24050166 MS.gene45095:CDS 55.0%
! TGCGTTCATTGGTGCTTGCC+AGG - chr5.3:24050685-24050704 MS.gene45095:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 24049275 24050762 24049275 ID=MS.gene45095
chr5.3 mRNA 24049275 24050762 24049275 ID=MS.gene45095.t1;Parent=MS.gene45095
chr5.3 exon 24050543 24050762 24050543 ID=MS.gene45095.t1.exon1;Parent=MS.gene45095.t1
chr5.3 CDS 24050543 24050762 24050543 ID=cds.MS.gene45095.t1;Parent=MS.gene45095.t1
chr5.3 exon 24049275 24050362 24049275 ID=MS.gene45095.t1.exon2;Parent=MS.gene45095.t1
chr5.3 CDS 24049275 24050362 24049275 ID=cds.MS.gene45095.t1;Parent=MS.gene45095.t1
Gene Sequence

>MS.gene45095

ATGGGAAGCACAAACCCCTTCAATGACAATCTTTCCTCCTTCAATAAGAAATTTTTTCATTTGACTCTTTATTCATTGCTTCCTATATCCCTTCTTTGTTTATACTTCATCATTTTGTCCCTTACTCCTTTTGCACCAACTAAGCTGCTTCATTCTACCACCATTTCCACCTACCACTATTCATCTACTTACTCTTCTCCTCCTTCAACTTCTTTTGTAGGTATATGCATGCATAATTAACTTCTAGTTGAGATTCACATGAACTCTAACTTCTATTCATCTACTTTTTTTTAAAAAAAAAAAATTATGCTAGTTGAGATTCACATGAACTCTAACTAGCAATTTGTTACTATTAAGGACTTCTTGGTTTACTAATTTTTATGTAATATATTTTTTGCAGGGAAGAACAAGGTTTATGATTATGAGAAATCATGTGACTATTCAAATGGAGATTGGGTTAGTGACATGAGAGGCCCTATATACAATGTCACCACATGTGGCACAATCAAAGAAAGTGAGAGATGCATCACAAATGGTAGACCTGATTTAGGTTATCTTTATTGGAGATGGAAGCCAAGTGAGTGTAATCTACCAAGGTTTGAACCAAGCACTTTCCTCAATCTCATTAAGAACAAACATATAGCTTTTGTTGGGGATTCTTTGGCTAGGAACCAATTAGAGTCACTTCTTTGCATGTTATTGACTACTTCAACACCTAACCTTGTTTACCAAGATGGCAAGTCCAATAAATTTCGTAAGTGGCACTTTCTTTCTCATAATGCTACCTTTTCTTTGTATTGGTCCCCATTTCTAGTGCATGGTGTTGAAAGATCAAATGAAGGGAATTATTATAATACAATGTTTTTGGATCTTGTCAATGAGAGGTGGGCCAAGGATATTGATCAAATGGACATGATTGTTATATCAACCGGGCATTGGTTTTTGCTTCCTTCAATTTACTATGAGAGTGATTTAGTTCTTGGTAGCTTGAATTGTCCCGAGTTTAATCATACTCAAATTGATTTTTATGTTCCATTAAGAAAGGCTTTGAGGACTACCCTAAATAGTATAATTGAGAGAAAAGTTGCTAAAGGAAATGGAATTGATGTGATTGTGAAAACATTTTCACCTGCTCATTTTGAAGGTGATTGGGATAAGGCTGGTACTTGTTCAAAGACTAAGCCTTATAGAAAAGAGGATAAGATAGTTGGAGGAATGGATGGTGAGATTAGAAGGATTGTGATAGATGAAGTGGAAAGTGCCAAGGTAAAAGCTAATAAATTTGAAGGTATTAGACTTGAGGTGTTGGATGTAACTAAATTGGCATTATTGAGACCAGATGGTCATCCAGGTCCTTACATGAATCCTTTTCCATTTGCAAATGGAGTTCCAAAATATGTACAAAATGATTGCGTTCATTGGTGCTTGCCAGGACCTATAGATACTTGGAATGAGATTTTTGTAGAGATGATGAAGAAGTGGGAGTAA
Protein sequence

>MS.gene45095.t1

MGSTNPFNDNLSSFNKKFFHLTLYSLLPISLLCLYFIILSLTPFAPTKLLHSTTISTYHYSSTYSSPPSTSFVGKNKVYDYEKSCDYSNGDWVSDMRGPIYNVTTCGTIKESERCITNGRPDLGYLYWRWKPSECNLPRFEPSTFLNLIKNKHIAFVGDSLARNQLESLLCMLLTTSTPNLVYQDGKSNKFRKWHFLSHNATFSLYWSPFLVHGVERSNEGNYYNTMFLDLVNERWAKDIDQMDMIVISTGHWFLLPSIYYESDLVLGSLNCPEFNHTQIDFYVPLRKALRTTLNSIIERKVAKGNGIDVIVKTFSPAHFEGDWDKAGTCSKTKPYRKEDKIVGGMDGEIRRIVIDEVESAKVKANKFEGIRLEVLDVTKLALLRPDGHPGPYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEIFVEMMKKWE