Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45324.t1 | XP_028762943.1 | 61.4 | 350 | 81 | 3 | 1 | 298 | 1 | 348 | 1.50E-112 | 416 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45324.t1 | A0A1U8KII2 | 56.5 | 336 | 94 | 3 | 1 | 284 | 1 | 336 | 6.0e-100 | 373.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053974 | MS.gene45324 | 0.800517 | 1.39E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene45324 | 0.809955 | 1.47E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene45324 | 0.804187 | 2.44E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene45324 | 0.826033 | 3.39E-54 | -1.69E-46 |
| MS.gene059272 | MS.gene45324 | 0.842753 | 2.13E-58 | -1.69E-46 |
| MS.gene060167 | MS.gene45324 | 0.80068 | 1.29E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45324.t1 | MTR_7g062720 | 65.152 | 132 | 46 | 0 | 1 | 132 | 1 | 132 | 1.85e-59 | 187 |
| MS.gene45324.t1 | MTR_5g061150 | 62.205 | 127 | 48 | 0 | 20 | 146 | 10 | 136 | 3.28e-48 | 157 |
| MS.gene45324.t1 | MTR_4g037355 | 50.000 | 116 | 17 | 2 | 151 | 225 | 1 | 116 | 1.10e-26 | 102 |
| MS.gene45324.t1 | MTR_4g112730 | 73.770 | 61 | 16 | 0 | 138 | 198 | 1 | 61 | 9.47e-25 | 95.9 |
| MS.gene45324.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 218 | 298 | 5 | 80 | 2.75e-23 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCTCTTAATATTTC+TGG | 0.080746 | 4.3:-53745219 | MS.gene45324:CDS |
| TTCGGCATTTAAATGAATTT+TGG | 0.166319 | 4.3:+53745167 | None:intergenic |
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 4.3:-53744324 | MS.gene45324:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 4.3:-53744332 | MS.gene45324:CDS |
| ATGGTTATATTTGCGTTTAC+AGG | 0.259438 | 4.3:-53744944 | MS.gene45324:CDS |
| AAATTGTCCCAAAGATCTTT+TGG | 0.267031 | 4.3:+53745021 | None:intergenic |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 4.3:-53745205 | MS.gene45324:CDS |
| TAGAAGATCCAAAAGATCTT+TGG | 0.283634 | 4.3:-53745029 | MS.gene45324:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 4.3:+53744170 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 4.3:+53744355 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 4.3:-53744272 | MS.gene45324:CDS |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 4.3:-53745200 | MS.gene45324:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.353242 | 4.3:-53745199 | MS.gene45324:CDS |
| AACCATTCATTACGAGCATT+AGG | 0.386252 | 4.3:+53744961 | None:intergenic |
| GAAAATGATGAAGATGATTA+TGG | 0.391347 | 4.3:-53744280 | MS.gene45324:CDS |
| AGAAGATCCAAAAGATCTTT+GGG | 0.400056 | 4.3:-53745028 | MS.gene45324:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 4.3:-53744403 | MS.gene45324:CDS |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 4.3:-53745076 | MS.gene45324:CDS |
| ATTAACACTGTGTGGAGAAA+AGG | 0.440407 | 4.3:-53744869 | MS.gene45324:CDS |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 4.3:-53745115 | MS.gene45324:CDS |
| AATTGTATCTCCATGCCCTT+CGG | 0.460742 | 4.3:+53745149 | None:intergenic |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 4.3:+53744242 | None:intergenic |
| GCAGCAACAATACCGTGAAA+AGG | 0.470239 | 4.3:-53744785 | MS.gene45324:CDS |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 4.3:-53744454 | MS.gene45324:CDS |
| AATCACTCGGCTCGTCCCAC+TGG | 0.475930 | 4.3:-53744691 | MS.gene45324:CDS |
| GCTACATTCACTTCTGGGAA+TGG | 0.483138 | 4.3:+53744661 | None:intergenic |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 4.3:-53744323 | MS.gene45324:CDS |
| TACATTAGATGCATGAAAAG+TGG | 0.490606 | 4.3:+53744813 | None:intergenic |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 4.3:-53745159 | MS.gene45324:CDS |
| CAGCAACAATACCGTGAAAA+GGG | 0.498612 | 4.3:-53744784 | MS.gene45324:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 4.3:-53744396 | MS.gene45324:CDS |
| TTCCTAATGCTCGTAATGAA+TGG | 0.506622 | 4.3:-53744963 | MS.gene45324:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 4.3:-53744188 | MS.gene45324:CDS |
| CATGGAGATACAATTAAAGA+AGG | 0.533844 | 4.3:-53745141 | MS.gene45324:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.537172 | 4.3:-53745165 | MS.gene45324:CDS |
| CTTAATATCATGCCTGCTTG+TGG | 0.567363 | 4.3:-53744743 | MS.gene45324:CDS |
| GTCTGTAATCGAATATAACT+CGG | 0.574961 | 4.3:-53744914 | MS.gene45324:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 4.3:-53745109 | MS.gene45324:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 4.3:-53744412 | MS.gene45324:CDS |
| ATTCATTACGAGCATTAGGA+AGG | 0.589984 | 4.3:+53744965 | None:intergenic |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 4.3:+53745076 | None:intergenic |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 4.3:-53744409 | MS.gene45324:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 4.3:+53744243 | None:intergenic |
| AAATTCATTTAAATGCCGAA+GGG | 0.631745 | 4.3:-53745164 | MS.gene45324:CDS |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 4.3:+53745086 | None:intergenic |
| ACTTCTCTATTAACACTGTG+TGG | 0.668278 | 4.3:-53744877 | MS.gene45324:CDS |
| TGGGAATGGAGCACTACCAG+TGG | 0.672955 | 4.3:+53744675 | None:intergenic |
| GGGAATGGAGCACTACCAGT+GGG | 0.676344 | 4.3:+53744676 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | - | chr4.3:53744972-53744991 | MS.gene45324:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr4.3:53745103-53745122 | MS.gene45324:CDS | 20.0% |
| !!! | TATTTCTGGAAATAATTATC+TGG | - | chr4.3:53744221-53744240 | MS.gene45324:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | - | chr4.3:53744261-53744280 | MS.gene45324:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | - | chr4.3:53744262-53744281 | MS.gene45324:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr4.3:53745102-53745121 | MS.gene45324:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | - | chr4.3:53745146-53745165 | MS.gene45324:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | - | chr4.3:53745154-53745173 | MS.gene45324:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | + | chr4.3:53745074-53745093 | None:intergenic | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | + | chr4.3:53745235-53745254 | None:intergenic | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | + | chr4.3:53744962-53744981 | None:intergenic | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | + | chr4.3:53744262-53744281 | None:intergenic | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | + | chr4.3:53744365-53744384 | None:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | + | chr4.3:53744938-53744957 | None:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | - | chr4.3:53744398-53744417 | MS.gene45324:CDS | 30.0% | |
| ATGGTTATATTTGCGTTTAC+AGG | - | chr4.3:53744482-53744501 | MS.gene45324:intron | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | - | chr4.3:53744311-53744330 | MS.gene45324:CDS | 30.0% | |
| CATGGAGATACAATTAAAGA+AGG | - | chr4.3:53744285-53744304 | MS.gene45324:CDS | 30.0% | |
| CATTAGAGATGAAAATATCC+AGG | - | chr4.3:53745238-53745257 | MS.gene45324:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr4.3:53745094-53745113 | MS.gene45324:CDS | 30.0% | |
| GTCTGTAATCGAATATAACT+CGG | - | chr4.3:53744512-53744531 | MS.gene45324:intron | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | + | chr4.3:53744616-53744635 | None:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | - | chr4.3:53744397-53744416 | MS.gene45324:CDS | 30.0% | |
| ! | AAATTGTCCCAAAGATCTTT+TGG | + | chr4.3:53744408-53744427 | None:intergenic | 30.0% |
| ! | AAGATCTTTTGGATCTTCTA+TGG | + | chr4.3:53744397-53744416 | None:intergenic | 30.0% |
| ! | GCTTTTGATGAAAAATCACT+CGG | - | chr4.3:53744722-53744741 | MS.gene45324:CDS | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | - | chr4.3:53744227-53744246 | MS.gene45324:CDS | 30.0% |
| ! | TTTGAGGCTCTTAATATTTC+TGG | - | chr4.3:53744207-53744226 | MS.gene45324:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | + | chr4.3:53744362-53744381 | None:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | - | chr4.3:53744888-53744907 | MS.gene45324:CDS | 30.0% |
| !!! | ACCTTTTAAAACCCTTTTCA+CGG | + | chr4.3:53744656-53744675 | None:intergenic | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr4.3:53745097-53745116 | None:intergenic | 30.0% |
| AACCATTCATTACGAGCATT+AGG | + | chr4.3:53744468-53744487 | None:intergenic | 35.0% | |
| AACTGAACAACAAAAGGCTA+AGG | - | chr4.3:53744317-53744336 | MS.gene45324:CDS | 35.0% | |
| ACTTCTCTATTAACACTGTG+TGG | - | chr4.3:53744549-53744568 | MS.gene45324:intron | 35.0% | |
| ATTAACACTGTGTGGAGAAA+AGG | - | chr4.3:53744557-53744576 | MS.gene45324:intron | 35.0% | |
| ATTCATTACGAGCATTAGGA+AGG | + | chr4.3:53744464-53744483 | None:intergenic | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | - | chr4.3:53744226-53744245 | MS.gene45324:CDS | 35.0% | |
| TTCCTAATGCTCGTAATGAA+TGG | - | chr4.3:53744463-53744482 | MS.gene45324:CDS | 35.0% | |
| ! | AGATGGTCAATCTTTTCATC+AGG | + | chr4.3:53745218-53745237 | None:intergenic | 35.0% |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | + | chr4.3:53744194-53744213 | None:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | - | chr4.3:53744191-53744210 | MS.gene45324:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | + | chr4.3:53744392-53744411 | None:intergenic | 35.0% |
| !!! | ACCGTGAAAAGGGTTTTAAA+AGG | - | chr4.3:53744652-53744671 | MS.gene45324:intron | 35.0% |
| AATTGTATCTCCATGCCCTT+CGG | + | chr4.3:53744280-53744299 | None:intergenic | 40.0% | |
| AGAAATCAGCAGCATCTAAG+TGG | + | chr4.3:53745187-53745206 | None:intergenic | 40.0% | |
| CAGCAACAATACCGTGAAAA+GGG | - | chr4.3:53744642-53744661 | MS.gene45324:intron | 40.0% | |
| CTTAATATCATGCCTGCTTG+TGG | - | chr4.3:53744683-53744702 | MS.gene45324:CDS | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | + | chr4.3:53745186-53745205 | None:intergenic | 40.0% | |
| GATCACTATGGACAAAATCG+TGG | - | chr4.3:53744795-53744814 | MS.gene45324:CDS | 40.0% | |
| GTAGCAACACATGATCACTA+TGG | - | chr4.3:53744783-53744802 | MS.gene45324:CDS | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | + | chr4.3:53744446-53744465 | None:intergenic | 40.0% |
| ! | GTGTTGCTACATTCACTTCT+GGG | + | chr4.3:53744773-53744792 | None:intergenic | 40.0% |
| ! | TATTTTGTTCTGCCACAAGC+AGG | + | chr4.3:53744698-53744717 | None:intergenic | 40.0% |
| ! | TGTGTTGCTACATTCACTTC+TGG | + | chr4.3:53744774-53744793 | None:intergenic | 40.0% |
| !! | AGTATTTGCTATCGTTGTGG+AGG | - | chr4.3:53745017-53745036 | MS.gene45324:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr4.3:53745014-53745033 | MS.gene45324:CDS | 40.0% |
| ACGTGGTCGTGTTTATGGAA+GGG | - | chr4.3:53744854-53744873 | MS.gene45324:CDS | 45.0% | |
| CATTTAAATGCCGAAGGGCA+TGG | - | chr4.3:53744267-53744286 | MS.gene45324:CDS | 45.0% | |
| GCAGCAACAATACCGTGAAA+AGG | - | chr4.3:53744641-53744660 | MS.gene45324:intron | 45.0% | |
| GTTGTGGAGGAAAAGGTCAT+TGG | - | chr4.3:53745030-53745049 | MS.gene45324:CDS | 45.0% | |
| TATGGACAAAATCGTGGTCG+TGG | - | chr4.3:53744801-53744820 | MS.gene45324:CDS | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | - | chr4.3:53745023-53745042 | MS.gene45324:CDS | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | + | chr4.3:53744768-53744787 | None:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | + | chr4.3:53744343-53744362 | None:intergenic | 45.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | + | chr4.3:53745063-53745082 | None:intergenic | 45.0% |
| CCTTCGTCACCACCTTCATA+AGG | - | chr4.3:53744350-53744369 | MS.gene45324:CDS | 50.0% | |
| CGTGGTCGTGTTTATGGAAG+GGG | - | chr4.3:53744855-53744874 | MS.gene45324:CDS | 50.0% | |
| GACGTGGTCGTGTTTATGGA+AGG | - | chr4.3:53744853-53744872 | MS.gene45324:CDS | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | + | chr4.3:53744353-53744372 | None:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | - | chr4.3:53744867-53744886 | MS.gene45324:CDS | 50.0% |
| CGTGGACGTGGTCGTGTTTA+TGG | - | chr4.3:53744849-53744868 | MS.gene45324:CDS | 55.0% | |
| CGTGTTTATGGAAGGGGACG+TGG | - | chr4.3:53744861-53744880 | MS.gene45324:CDS | 55.0% | |
| ! | GGGAATGGAGCACTACCAGT+GGG | + | chr4.3:53744753-53744772 | None:intergenic | 55.0% |
| ! | TGGGAATGGAGCACTACCAG+TGG | + | chr4.3:53744754-53744773 | None:intergenic | 55.0% |
| CAAAATCGTGGTCGTGGCCG+TGG | - | chr4.3:53744807-53744826 | MS.gene45324:CDS | 60.0% | |
| CACGTCCACGACCATGACCA+CGG | + | chr4.3:53744827-53744846 | None:intergenic | 60.0% | |
| ! | AATCACTCGGCTCGTCCCAC+TGG | - | chr4.3:53744735-53744754 | MS.gene45324:CDS | 60.0% |
| CATGGTCGTGGACGTGGACG+TGG | - | chr4.3:53744831-53744850 | MS.gene45324:CDS | 65.0% | |
| CGTGGTCATGGTCGTGGACG+TGG | - | chr4.3:53744825-53744844 | MS.gene45324:CDS | 65.0% | |
| CGTGGACGTGGACGTGGACG+TGG | - | chr4.3:53744837-53744856 | MS.gene45324:CDS | 70.0% | |
| CGTGGCCGTGGTCATGGTCG+TGG | - | chr4.3:53744819-53744838 | MS.gene45324:CDS | 70.0% | |
| CGTGGTCGTGGCCGTGGTCA+TGG | - | chr4.3:53744813-53744832 | MS.gene45324:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 53744183 | 53745265 | 53744183 | ID=MS.gene45324 |
| chr4.3 | mRNA | 53744183 | 53745265 | 53744183 | ID=MS.gene45324.t1;Parent=MS.gene45324 |
| chr4.3 | exon | 53744672 | 53745265 | 53744672 | ID=MS.gene45324.t1.exon1;Parent=MS.gene45324.t1 |
| chr4.3 | CDS | 53744672 | 53745265 | 53744672 | ID=cds.MS.gene45324.t1;Parent=MS.gene45324.t1 |
| chr4.3 | exon | 53744183 | 53744485 | 53744183 | ID=MS.gene45324.t1.exon2;Parent=MS.gene45324.t1 |
| chr4.3 | CDS | 53744183 | 53744485 | 53744183 | ID=cds.MS.gene45324.t1;Parent=MS.gene45324.t1 |
| Gene Sequence |
| Protein sequence |
