Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45385.t1 | RHN62374.1 | 91 | 277 | 25 | 0 | 177 | 453 | 1 | 277 | 4.30E-132 | 481.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45385.t1 | Q688X9 | 48.0 | 173 | 59 | 4 | 1 | 147 | 1 | 168 | 1.8e-33 | 145.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45385.t1 | A0A396IDQ2 | 91.0 | 277 | 25 | 0 | 177 | 453 | 1 | 277 | 3.1e-132 | 481.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057060 | MS.gene45385 | -0.804073 | 2.57E-49 | -1.69E-46 |
| MS.gene060165 | MS.gene45385 | -0.808256 | 3.39E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45385.t1 | MTR_3g019330 | 83.103 | 290 | 49 | 0 | 159 | 448 | 37 | 326 | 7.32e-172 | 490 |
| MS.gene45385.t1 | MTR_4g088440 | 90.875 | 263 | 24 | 0 | 191 | 453 | 1 | 263 | 1.60e-159 | 451 |
| MS.gene45385.t1 | MTR_4g088430 | 96.711 | 152 | 4 | 1 | 1 | 152 | 1 | 151 | 8.46e-105 | 310 |
| MS.gene45385.t1 | MTR_4g088440 | 91.848 | 184 | 15 | 0 | 177 | 360 | 1 | 184 | 3.13e-103 | 305 |
| MS.gene45385.t1 | MTR_1g083780 | 33.077 | 130 | 54 | 3 | 31 | 134 | 30 | 152 | 1.06e-13 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45385.t1 | AT1G50910 | 55.600 | 250 | 107 | 3 | 179 | 426 | 4 | 251 | 1.04e-72 | 238 |
| MS.gene45385.t1 | AT3G05700 | 33.333 | 162 | 74 | 4 | 1 | 134 | 1 | 156 | 4.46e-18 | 83.6 |
| MS.gene45385.t1 | AT3G05700 | 33.333 | 162 | 74 | 4 | 1 | 134 | 1 | 156 | 5.11e-18 | 82.8 |
| MS.gene45385.t1 | AT1G50910 | 46.000 | 100 | 50 | 3 | 329 | 426 | 11 | 108 | 1.08e-16 | 81.6 |
| MS.gene45385.t1 | AT5G26990 | 29.630 | 162 | 79 | 4 | 1 | 134 | 1 | 155 | 1.54e-13 | 70.1 |
| MS.gene45385.t1 | AT1G56280 | 32.540 | 126 | 56 | 4 | 31 | 134 | 27 | 145 | 2.03e-13 | 69.7 |
| MS.gene45385.t1 | AT1G56280 | 32.283 | 127 | 56 | 4 | 31 | 134 | 20 | 139 | 2.66e-13 | 68.9 |
| MS.gene45385.t1 | AT5G49230 | 32.576 | 132 | 58 | 4 | 31 | 139 | 28 | 151 | 1.92e-11 | 63.9 |
| MS.gene45385.t1 | AT3G06760 | 32.090 | 134 | 60 | 3 | 32 | 139 | 33 | 161 | 3.94e-11 | 63.2 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGTCAAGTTGAGGATTT+TGG | 0.209491 | 4.4:-24456227 | MS.gene45385:CDS |
| CAGTCAGACGTTGTCTCTTT+TGG | 0.211711 | 4.4:-24463825 | MS.gene45385:CDS |
| TAGATGAGAGGAAGCATAAA+AGG | 0.211889 | 4.4:-24456256 | MS.gene45385:CDS |
| AATCACCCAACTTCTCATTT+AGG | 0.253905 | 4.4:-24465481 | MS.gene45385:intron |
| TACATCGCAGCCTTCAAAAT+TGG | 0.278836 | 4.4:+24457663 | None:intergenic |
| CATTTGCAGGTGCTTCTTAA+TGG | 0.280797 | 4.4:-24463782 | MS.gene45385:CDS |
| CTTCAAAAGATGCTGGAAAT+AGG | 0.288940 | 4.4:+24459670 | None:intergenic |
| AACTTCAAGTTCAATGTGTT+TGG | 0.299916 | 4.4:-24460729 | MS.gene45385:CDS |
| AACACTATTGAGTCGATTCT+TGG | 0.308082 | 4.4:-24456167 | MS.gene45385:CDS |
| AAAAGACGAATCTTCTGTTT+TGG | 0.324292 | 4.4:+24459328 | None:intergenic |
| CTTCAATGGATTCTGAGTTC+TGG | 0.334015 | 4.4:-24465549 | None:intergenic |
| TGTTTCATGACAGCTTAAAA+AGG | 0.336884 | 4.4:-24458647 | MS.gene45385:intron |
| GCTAGCGCGTCTACGAGTTT+TGG | 0.353240 | 4.4:-24456836 | MS.gene45385:CDS |
| CAATACGGACAAGGGAAATC+TGG | 0.376334 | 4.4:+24465249 | None:intergenic |
| AGAGACAACGTCTGACTGTT+TGG | 0.384231 | 4.4:+24463830 | None:intergenic |
| AAGTTCAATGTGTTTGGAAT+TGG | 0.385432 | 4.4:-24460723 | MS.gene45385:CDS |
| CTTCTTAATGGTGGTGGTGG+TGG | 0.390196 | 4.4:-24463770 | MS.gene45385:CDS |
| ACATCTACTTCACCACTCTC+TGG | 0.392256 | 4.4:+24455431 | None:intergenic |
| ACACTATTGAGTCGATTCTT+GGG | 0.399329 | 4.4:-24456166 | MS.gene45385:CDS |
| TCTACGTGAAGCTCATTTGC+AGG | 0.400989 | 4.4:-24463795 | MS.gene45385:CDS |
| TACAAACCTAAATGAGAAGT+TGG | 0.412351 | 4.4:+24465475 | None:intergenic |
| ATGTTACATTAGAGTGTGTC+TGG | 0.424478 | 4.4:-24456298 | MS.gene45385:CDS |
| AGATGAGAGGAAGCATAAAA+GGG | 0.441741 | 4.4:-24456255 | MS.gene45385:CDS |
| GGGACTTTCTGTGACAATCA+TGG | 0.457633 | 4.4:-24456863 | MS.gene45385:CDS |
| ATTTCTTCTGCTTCGCATGC+AGG | 0.460602 | 4.4:+24463674 | None:intergenic |
| CAATCTATTAAAATAACTCA+TGG | 0.479405 | 4.4:-24456512 | MS.gene45385:CDS |
| GTGCTTCTTAATGGTGGTGG+TGG | 0.480850 | 4.4:-24463773 | MS.gene45385:CDS |
| CAGGTGCTTCTTAATGGTGG+TGG | 0.492363 | 4.4:-24463776 | MS.gene45385:CDS |
| ATTAAATTGCAAGGATTCGA+TGG | 0.492656 | 4.4:-24459810 | MS.gene45385:CDS |
| GGATTTAAATCTTCTACAGT+TGG | 0.499368 | 4.4:-24459404 | MS.gene45385:CDS |
| ATGTGGAATACTTGAAAGAT+TGG | 0.505870 | 4.4:-24459726 | MS.gene45385:CDS |
| AGTAGATGTTGCAGCTAAAG+AGG | 0.506345 | 4.4:-24455417 | MS.gene45385:CDS |
| TGCATACCTTCAAAAGATGC+TGG | 0.509973 | 4.4:+24459663 | None:intergenic |
| AATCTATTAAAATAACTCAT+GGG | 0.513875 | 4.4:-24456511 | MS.gene45385:CDS |
| CATCTACTTCACCACTCTCT+GGG | 0.521488 | 4.4:+24455432 | None:intergenic |
| TGTTATGAAGAGTTTGACAT+AGG | 0.521568 | 4.4:-24465228 | MS.gene45385:CDS |
| ACATATCAGAATTATATCCT+TGG | 0.521831 | 4.4:+24455382 | None:intergenic |
| ACAAACCTAAATGAGAAGTT+GGG | 0.527430 | 4.4:+24465476 | None:intergenic |
| ATCCTTGCAATTTAATCCAA+CGG | 0.534772 | 4.4:+24459817 | None:intergenic |
| TTCATGTGAATCAAGAGTTA+CGG | 0.536211 | 4.4:-24465175 | MS.gene45385:intron |
| GAATTATATCCTTGGCATGA+GGG | 0.538937 | 4.4:+24455390 | None:intergenic |
| ATCTATTAAAATAACTCATG+GGG | 0.540345 | 4.4:-24456510 | MS.gene45385:CDS |
| ACAATGGAGCTGCGAAAGCA+TGG | 0.545280 | 4.4:+24459751 | None:intergenic |
| TCAAACTCTTCATAACAATA+CGG | 0.545941 | 4.4:+24465234 | None:intergenic |
| GTAGATCATAGTTCTGCCGT+TGG | 0.547170 | 4.4:-24459833 | MS.gene45385:intron |
| CACTATTGAGTCGATTCTTG+GGG | 0.548086 | 4.4:-24456165 | MS.gene45385:CDS |
| TCGCAGCTCCATTGTGAATG+TGG | 0.550317 | 4.4:-24459743 | MS.gene45385:CDS |
| TCATGTTTCAATGTTATCAA+AGG | 0.550576 | 4.4:-24459358 | MS.gene45385:CDS |
| TGCCGTTGGATTAAATTGCA+AGG | 0.554378 | 4.4:-24459819 | MS.gene45385:CDS |
| GAATGGATCAGCAGACGCAT+TGG | 0.556423 | 4.4:+24463721 | None:intergenic |
| AGGGTAATTCAAAACAAAAG+AGG | 0.557118 | 4.4:+24463694 | None:intergenic |
| CACCACTCTCTGGGTCAAGT+TGG | 0.559539 | 4.4:+24455441 | None:intergenic |
| AAAACAAAAGAGGATAGGAA+TGG | 0.563259 | 4.4:+24463704 | None:intergenic |
| CAAGTATTCCACATTCACAA+TGG | 0.564121 | 4.4:+24459735 | None:intergenic |
| AATTATATCCTTGGCATGAG+GGG | 0.564514 | 4.4:+24455391 | None:intergenic |
| TTTCTTCTGCTTCGCATGCA+GGG | 0.565302 | 4.4:+24463675 | None:intergenic |
| TAATCATTATCATCACTACC+AGG | 0.567418 | 4.4:+24456320 | None:intergenic |
| ATAAGTATATATTAGATGAG+AGG | 0.567492 | 4.4:-24456268 | MS.gene45385:CDS |
| GGAGAATGAAAGTCAAGTTG+AGG | 0.573104 | 4.4:-24456234 | MS.gene45385:CDS |
| GTTTGGTCATTAGGTGCGGA+GGG | 0.573872 | 4.4:-24459425 | MS.gene45385:intron |
| AAGCGTATAGTGGCGCTTAG+CGG | 0.574792 | 4.4:+24465510 | None:intergenic |
| AATTCAAAACAAAAGAGGAT+AGG | 0.575772 | 4.4:+24463699 | None:intergenic |
| TTGCAGGTGCTTCTTAATGG+TGG | 0.575859 | 4.4:-24463779 | MS.gene45385:CDS |
| TTAAATTGCAAGGATTCGAT+GGG | 0.589402 | 4.4:-24459809 | MS.gene45385:CDS |
| GATTGTGTTGAAGCGTATAG+TGG | 0.590099 | 4.4:+24465500 | None:intergenic |
| TTACTGTGTTACTGTGCGAC+CGG | 0.593038 | 4.4:+24463744 | None:intergenic |
| GGTTTGGTCATTAGGTGCGG+AGG | 0.608260 | 4.4:-24459426 | MS.gene45385:intron |
| TACTGAATTTGAAACAGTCG+AGG | 0.609403 | 4.4:-24459786 | MS.gene45385:CDS |
| AGAATTATATCCTTGGCATG+AGG | 0.615696 | 4.4:+24455389 | None:intergenic |
| GAAAATGCCTTGATGAAAAG+CGG | 0.616892 | 4.4:-24458623 | MS.gene45385:CDS |
| CTTCATAACAATACGGACAA+GGG | 0.621360 | 4.4:+24465241 | None:intergenic |
| AGAAGAAATCTCTAAATCTG+TGG | 0.631495 | 4.4:-24463660 | MS.gene45385:intron |
| GACCAACTTGACCCAGAGAG+TGG | 0.632334 | 4.4:-24455443 | MS.gene45385:CDS |
| ATTATATCCTTGGCATGAGG+GGG | 0.638226 | 4.4:+24455392 | None:intergenic |
| TCTTCATAACAATACGGACA+AGG | 0.638894 | 4.4:+24465240 | None:intergenic |
| TCTACAGTTGGCAAACGTAG+AGG | 0.644321 | 4.4:-24459392 | MS.gene45385:CDS |
| CTTAGCGGCAGCAAGACGAG+AGG | 0.656188 | 4.4:+24465525 | None:intergenic |
| ACTATTGAGTCGATTCTTGG+GGG | 0.665684 | 4.4:-24456164 | MS.gene45385:intron |
| ATCTGCACGACTACTAGCAG+AGG | 0.666792 | 4.4:-24458577 | MS.gene45385:intron |
| GATGCTGGAAATAGGAAGAG+CGG | 0.678399 | 4.4:+24459678 | None:intergenic |
| AATTTGAAACAGTCGAGGGG+CGG | 0.679861 | 4.4:-24459781 | MS.gene45385:CDS |
| TCTATTAAAATAACTCATGG+GGG | 0.690281 | 4.4:-24456509 | MS.gene45385:CDS |
| ACTGAATTTGAAACAGTCGA+GGG | 0.690372 | 4.4:-24459785 | MS.gene45385:CDS |
| CTTACATACAGAGAAGGCGC+AGG | 0.692896 | 4.4:-24463871 | MS.gene45385:intron |
| AATCATTATCATCACTACCA+GGG | 0.697644 | 4.4:+24456321 | None:intergenic |
| CTGAATTTGAAACAGTCGAG+GGG | 0.699477 | 4.4:-24459784 | MS.gene45385:CDS |
| GCTGGAAATAGGAAGAGCGG+CGG | 0.714092 | 4.4:+24459681 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 24455379 | 24465566 | 24455379 | ID=MS.gene45385 |
| chr4.4 | mRNA | 24455379 | 24465566 | 24455379 | ID=MS.gene45385.t1;Parent=MS.gene45385 |
| chr4.4 | exon | 24465482 | 24465566 | 24465482 | ID=MS.gene45385.t1.exon1;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24465482 | 24465566 | 24465482 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24465176 | 24465315 | 24465176 | ID=MS.gene45385.t1.exon2;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24465176 | 24465315 | 24465176 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24463661 | 24463882 | 24463661 | ID=MS.gene45385.t1.exon3;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24463661 | 24463882 | 24463661 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24460715 | 24460757 | 24460715 | ID=MS.gene45385.t1.exon4;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24460715 | 24460757 | 24460715 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24459670 | 24459851 | 24459670 | ID=MS.gene45385.t1.exon5;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24459670 | 24459851 | 24459670 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24459322 | 24459434 | 24459322 | ID=MS.gene45385.t1.exon6;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24459322 | 24459434 | 24459322 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24458578 | 24458656 | 24458578 | ID=MS.gene45385.t1.exon7;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24458578 | 24458656 | 24458578 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24457666 | 24457694 | 24457666 | ID=MS.gene45385.t1.exon8;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24457666 | 24457694 | 24457666 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24456828 | 24456921 | 24456828 | ID=MS.gene45385.t1.exon9;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24456828 | 24456921 | 24456828 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24456502 | 24456591 | 24456502 | ID=MS.gene45385.t1.exon10;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24456502 | 24456591 | 24456502 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24456165 | 24456360 | 24456165 | ID=MS.gene45385.t1.exon11;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24456165 | 24456360 | 24456165 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| chr4.4 | exon | 24455379 | 24455467 | 24455379 | ID=MS.gene45385.t1.exon12;Parent=MS.gene45385.t1 |
| chr4.4 | CDS | 24455379 | 24455467 | 24455379 | ID=cds.MS.gene45385.t1;Parent=MS.gene45385.t1 |
| Gene Sequence |
| Protein sequence |
