Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45409.t1 | XP_024628414.1 | 98.1 | 54 | 1 | 0 | 1 | 54 | 2 | 55 | 2.60E-21 | 110.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45409.t1 | A0A396GEY0 | 98.1 | 54 | 1 | 0 | 1 | 54 | 2 | 55 | 1.9e-21 | 110.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene45409 | 0.800997 | 1.11E-48 | -1.69E-46 |
| MS.gene059128 | MS.gene45409 | 0.801712 | 7.91E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGATATGAACAATGAATT+TGG | 0.175410 | 8.3:+78687407 | None:intergenic |
| AGAGATTGGATGAGGAGATT+TGG | 0.369588 | 8.3:+78687346 | None:intergenic |
| GAGATATGAACAATGAATTT+GGG | 0.389271 | 8.3:+78687408 | None:intergenic |
| TGAAACAGAAGAAGGAAATC+AGG | 0.424697 | 8.3:+78687381 | None:intergenic |
| AATGTTATGCTGATATTGAC+AGG | 0.434302 | 8.3:-78687204 | MS.gene45409:intron |
| TGAGGTAAACATACAGAGAT+TGG | 0.453247 | 8.3:+78687332 | None:intergenic |
| TTGGATGAGGAGATTTGGCA+AGG | 0.528623 | 8.3:+78687351 | None:intergenic |
| AACAATGAATTTGGGTTCCG+AGG | 0.564501 | 8.3:+78687416 | None:intergenic |
| AAACATACAGAGATTGGATG+AGG | 0.575476 | 8.3:+78687338 | None:intergenic |
| GAGTGGTGTGAAACAGAAGA+AGG | 0.605127 | 8.3:+78687373 | None:intergenic |
| TGAGGAGATTTGGCAAGGAG+TGG | 0.633321 | 8.3:+78687356 | None:intergenic |
| TAGTTACAACAGTTTCACCT+CGG | 0.689854 | 8.3:-78687433 | MS.gene45409:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CACAAAAAACTTATAATAAG+AGG | + | chr8.3:78687379-78687398 | None:intergenic | 20.0% |
| ! | AGAGATATGAACAATGAATT+TGG | + | chr8.3:78687131-78687150 | None:intergenic | 25.0% |
| ! | CAAAAAATGAAATTGTTGTG+AGG | + | chr8.3:78687224-78687243 | None:intergenic | 25.0% |
| ! | GAGATATGAACAATGAATTT+GGG | + | chr8.3:78687130-78687149 | None:intergenic | 25.0% |
| !! | ATTTTGTACATGAAGATTTG+AGG | - | chr8.3:78687425-78687444 | MS.gene45409:CDS | 25.0% |
| AATGTTATGCTGATATTGAC+AGG | - | chr8.3:78687331-78687350 | MS.gene45409:intron | 30.0% | |
| CCAGAAATGAAAATCCAAAA+GGG | + | chr8.3:78687263-78687282 | None:intergenic | 30.0% | |
| ! | CCTTTTGGATTTTCATTTCT+GGG | - | chr8.3:78687261-78687280 | MS.gene45409:intron | 30.0% |
| !!! | GGGTTTTTCATTTTTTTTGC+AGG | - | chr8.3:78687281-78687300 | MS.gene45409:intron | 30.0% |
| AAACATACAGAGATTGGATG+AGG | + | chr8.3:78687200-78687219 | None:intergenic | 35.0% | |
| CCCAGAAATGAAAATCCAAA+AGG | + | chr8.3:78687264-78687283 | None:intergenic | 35.0% | |
| TAGTTACAACAGTTTCACCT+CGG | - | chr8.3:78687102-78687121 | MS.gene45409:intron | 35.0% | |
| TGAAACAGAAGAAGGAAATC+AGG | + | chr8.3:78687157-78687176 | None:intergenic | 35.0% | |
| TGAGGTAAACATACAGAGAT+TGG | + | chr8.3:78687206-78687225 | None:intergenic | 35.0% | |
| ! | CCCTTTTGGATTTTCATTTC+TGG | - | chr8.3:78687260-78687279 | MS.gene45409:intron | 35.0% |
| ! | TTCACTTTCTTTCACCCTTT+TGG | - | chr8.3:78687246-78687265 | MS.gene45409:intron | 35.0% |
| !! | TTTTGCAGGATGTTCAAGTT+AGG | - | chr8.3:78687295-78687314 | MS.gene45409:intron | 35.0% |
| AGAGATTGGATGAGGAGATT+TGG | + | chr8.3:78687192-78687211 | None:intergenic | 40.0% | |
| ! | AACAATGAATTTGGGTTCCG+AGG | + | chr8.3:78687122-78687141 | None:intergenic | 40.0% |
| GAGTGGTGTGAAACAGAAGA+AGG | + | chr8.3:78687165-78687184 | None:intergenic | 45.0% | |
| TTGGATGAGGAGATTTGGCA+AGG | + | chr8.3:78687187-78687206 | None:intergenic | 45.0% | |
| TGAGGAGATTTGGCAAGGAG+TGG | + | chr8.3:78687182-78687201 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 78687098 | 78687459 | 78687098 | ID=MS.gene45409 |
| chr8.3 | mRNA | 78687098 | 78687459 | 78687098 | ID=MS.gene45409.t1;Parent=MS.gene45409 |
| chr8.3 | exon | 78687346 | 78687459 | 78687346 | ID=MS.gene45409.t1.exon1;Parent=MS.gene45409.t1 |
| chr8.3 | CDS | 78687346 | 78687459 | 78687346 | ID=cds.MS.gene45409.t1;Parent=MS.gene45409.t1 |
| chr8.3 | exon | 78687205 | 78687254 | 78687205 | ID=MS.gene45409.t1.exon2;Parent=MS.gene45409.t1 |
| chr8.3 | CDS | 78687205 | 78687254 | 78687205 | ID=cds.MS.gene45409.t1;Parent=MS.gene45409.t1 |
| chr8.3 | exon | 78687098 | 78687110 | 78687098 | ID=MS.gene45409.t1.exon3;Parent=MS.gene45409.t1 |
| chr8.3 | CDS | 78687098 | 78687110 | 78687098 | ID=cds.MS.gene45409.t1;Parent=MS.gene45409.t1 |
| Gene Sequence |
| Protein sequence |
