AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45528

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45528.t1 MTR_2g097620 98.045 358 4 1 1 358 1 355 0.0 710
MS.gene45528.t1 MTR_4g064570 64.266 361 106 8 1 351 1 348 2.92e-150 428
MS.gene45528.t1 MTR_3g068005 51.163 344 129 5 9 317 2 341 5.68e-111 330
MS.gene45528.t1 MTR_5g080350 44.186 344 157 9 12 330 2 335 1.21e-82 256
MS.gene45528.t1 MTR_1g114320 32.558 258 149 8 14 258 13 258 3.59e-40 144
MS.gene45528.t1 MTR_5g088720 34.211 266 122 8 6 237 4 250 2.71e-38 139
MS.gene45528.t1 MTR_5g088720 34.221 263 120 8 9 237 2 245 3.80e-38 139
MS.gene45528.t1 MTR_5g088720 34.201 269 124 8 3 237 42 291 4.24e-38 140
MS.gene45528.t1 MTR_0067s0100 35.656 244 131 8 9 237 3 235 3.84e-36 132
MS.gene45528.t1 MTR_1g051105 31.169 231 127 5 9 237 4 204 6.22e-33 123
MS.gene45528.t1 MTR_6g079660 46.903 113 56 2 135 246 180 289 1.95e-31 122
MS.gene45528.t1 MTR_3g072630 29.412 255 141 6 14 237 20 266 1.57e-30 119
MS.gene45528.t1 MTR_3g072630 29.412 255 141 6 14 237 20 266 2.73e-30 118
MS.gene45528.t1 MTR_3g083480 42.520 127 61 3 135 257 188 306 3.92e-28 113
MS.gene45528.t1 MTR_1g114620 50.000 102 45 3 145 245 221 317 3.40e-26 107
MS.gene45528.t1 MTR_6g032960 41.026 117 63 3 124 240 174 284 6.34e-25 103
MS.gene45528.t1 MTR_2g007340 43.519 108 59 1 149 254 158 265 1.24e-23 99.8
MS.gene45528.t1 MTR_7g074170 43.564 101 53 2 145 244 300 397 1.24e-22 99.4
MS.gene45528.t1 MTR_4g110100 38.739 111 64 3 137 246 307 414 1.36e-22 98.6
MS.gene45528.t1 MTR_3g088080 43.902 82 44 1 157 238 5 84 2.84e-22 90.1
MS.gene45528.t1 MTR_6g016350 46.809 94 49 1 152 244 270 363 6.66e-20 90.1
MS.gene45528.t1 MTR_1g014710 36.893 103 62 1 146 245 184 286 2.57e-19 87.8
MS.gene45528.t1 MTR_5g041310 35.714 112 64 2 129 240 60 163 6.56e-18 82.0
MS.gene45528.t1 MTR_5g041330 40.000 80 45 1 162 241 78 154 7.39e-17 79.0
MS.gene45528.t1 MTR_2g061790 61.905 42 16 0 192 233 109 150 1.62e-14 72.4
MS.gene45528.t1 MTR_3g031000 41.667 72 34 2 167 238 279 342 1.11e-12 68.6
MS.gene45528.t1 MTR_5g022920 29.375 160 93 5 97 236 75 234 4.87e-12 65.5
MS.gene45528.t1 MTR_3g025510 35.938 64 41 0 170 233 83 146 5.03e-12 63.5
MS.gene45528.t1 MTR_4g080750 44.444 54 30 0 180 233 173 226 1.97e-11 64.3
MS.gene45528.t1 MTR_5g058530 36.364 88 40 3 179 258 210 289 2.30e-11 64.7
MS.gene45528.t1 MTR_1g095030 58.333 36 15 0 198 233 18 53 7.31e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45528.t1 AT5G59550 51.792 307 123 6 12 304 16 311 1.45e-92 283
MS.gene45528.t1 AT3G46620 52.459 305 122 8 13 304 30 324 8.23e-92 280
MS.gene45528.t1 AT5G59550 51.961 306 122 6 13 304 122 416 9.93e-92 284
MS.gene45528.t1 AT2G39720 44.985 329 116 10 10 293 2 310 2.09e-83 259
MS.gene45528.t1 AT2G39720 44.985 329 116 10 10 293 2 310 2.09e-83 259
MS.gene45528.t1 AT1G55530 31.541 279 139 8 11 245 6 276 9.47e-37 136
MS.gene45528.t1 AT1G55530 31.541 279 139 8 11 245 6 276 9.47e-37 136
MS.gene45528.t1 AT3G56580 34.836 244 121 6 13 237 7 231 6.17e-36 133
MS.gene45528.t1 AT3G56580 34.836 244 121 6 13 237 7 231 6.17e-36 133
MS.gene45528.t1 AT3G56580 34.836 244 121 6 13 237 7 231 6.17e-36 133
MS.gene45528.t1 AT2G40830 33.457 269 121 9 9 246 3 244 8.91e-36 133
MS.gene45528.t1 AT2G40830 33.457 269 121 9 9 246 3 244 8.91e-36 133
MS.gene45528.t1 AT2G40830 33.457 269 121 9 9 246 3 244 8.91e-36 133
MS.gene45528.t1 AT2G40830 33.457 269 121 9 9 246 3 244 8.91e-36 133
MS.gene45528.t1 AT1G55530 30.682 264 128 6 11 245 6 243 3.35e-35 131
MS.gene45528.t1 AT3G19950 31.321 265 157 7 3 246 10 270 1.37e-34 130
MS.gene45528.t1 AT3G19950 31.321 265 157 7 3 246 68 328 2.98e-34 130
MS.gene45528.t1 AT3G19950 31.321 265 157 7 3 246 68 328 2.98e-34 130
MS.gene45528.t1 AT3G13430 29.818 275 141 8 9 238 4 271 1.43e-31 121
MS.gene45528.t1 AT3G13430 29.818 275 141 8 9 238 4 271 1.43e-31 121
MS.gene45528.t1 AT3G13430 29.818 275 141 8 9 238 4 271 1.43e-31 121
MS.gene45528.t1 AT3G13430 29.818 275 141 8 9 238 4 271 1.43e-31 121
MS.gene45528.t1 AT3G10815 58.974 78 32 0 166 243 95 172 3.59e-28 109
MS.gene45528.t1 AT5G01980 42.735 117 64 1 128 244 289 402 4.81e-26 108
MS.gene45528.t1 AT5G08139 43.689 103 54 2 145 246 264 363 9.00e-26 107
MS.gene45528.t1 AT5G56340 44.231 104 50 2 135 238 210 305 1.66e-24 103
MS.gene45528.t1 AT1G60360 42.453 106 55 2 142 246 178 278 2.14e-24 102
MS.gene45528.t1 AT5G15820 44.681 94 51 1 154 246 252 345 5.22e-24 101
MS.gene45528.t1 AT3G02340 36.620 142 79 2 120 250 252 393 2.55e-23 100
MS.gene45528.t1 AT3G02340 36.620 142 79 2 120 250 252 393 2.55e-23 100
MS.gene45528.t1 AT4G26400 40.952 105 54 2 135 239 192 288 1.09e-22 97.8
MS.gene45528.t1 AT4G26400 40.952 105 54 2 135 239 192 288 1.09e-22 97.8
MS.gene45528.t1 AT5G64920 44.231 104 57 1 142 244 206 309 1.43e-22 97.4
MS.gene45528.t1 AT1G68180 53.521 71 33 0 166 236 112 182 2.91e-22 94.4
MS.gene45528.t1 AT3G60080 55.405 74 25 1 173 238 142 215 1.93e-21 93.6
MS.gene45528.t1 AT5G20910 35.398 113 64 3 136 245 177 283 3.52e-20 90.1
MS.gene45528.t1 AT5G60820 44.211 95 45 3 150 239 328 419 3.52e-20 91.7
MS.gene45528.t1 AT2G44330 42.157 102 45 2 173 260 71 172 3.59e-19 84.3
MS.gene45528.t1 AT1G26800 40.541 111 57 3 129 239 59 160 1.08e-17 80.9
MS.gene45528.t1 AT1G14200 29.448 163 85 3 75 237 22 154 2.61e-15 73.6
MS.gene45528.t1 AT5G02750 50.000 56 28 0 189 244 211 266 7.80e-15 74.3
MS.gene45528.t1 AT3G30460 43.478 69 35 2 174 238 77 145 4.65e-13 66.6
MS.gene45528.t1 AT3G30460 43.662 71 36 2 172 238 111 181 6.51e-13 67.0
MS.gene45528.t1 AT3G55530 34.211 76 50 0 158 233 177 252 8.43e-11 62.4

Find 101 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGGCGGGAGGTTTGTCTTC+TGG 0.283501 2.1:-6455807 None:intergenic
GCAAACTGCACAGTGAATTT+CGG 0.334109 2.1:-6456185 None:intergenic
AAGCGGTTGGATTGACGATA+TGG 0.341428 2.1:+6456383 MS.gene45528:CDS
GCGTCTGAGAGTAGAATTTC+CGG 0.352120 2.1:+6456346 MS.gene45528:CDS
ACCGCCGCCGGCGTCTGCAC+CGG 0.356131 2.1:-6455915 None:intergenic
GAGGACAAACGACGTTGTTT+TGG 0.359117 2.1:-6455703 None:intergenic
CATCCTCAACGACGTCGTTT+TGG 0.362996 2.1:+6455645 MS.gene45528:CDS
AGAACGATTACAGGATTAAA+AGG 0.369503 2.1:-6455860 None:intergenic
GCCGGCGTCTGCACCGGCAC+CGG 0.370020 2.1:-6455909 None:intergenic
CAAGAAGAAGAAGCAGAACT+TGG 0.386359 2.1:+6456662 MS.gene45528:CDS
TCTCTTCGACGAATCCGTTT+TGG 0.397147 2.1:-6455730 None:intergenic
CCGGCGGTGGTGTCTTCTCT+AGG 0.412632 2.1:-6455890 None:intergenic
GCCGGTGCAGACGCCGGCGG+CGG 0.414591 2.1:+6455914 MS.gene45528:CDS
TCGGAGCTATTACTCGGTTC+CGG 0.420389 2.1:+6456031 MS.gene45528:CDS
TTAGAGAGAGACGTGGAATT+CGG 0.425154 2.1:+6456557 MS.gene45528:CDS
TACTCGGTTCCGGCTTCGAT+CGG 0.428438 2.1:+6456041 MS.gene45528:CDS
GTCTGCACCGGCACCGGCGG+TGG 0.429367 2.1:-6455903 None:intergenic
TTCGGATTCGATTTCTGAAT+CGG 0.431784 2.1:-6456167 None:intergenic
GCAGACGCCGGCGGCGGTAG+CGG 0.432430 2.1:+6455920 MS.gene45528:CDS
GGTCCACGGAGAACGATTAC+AGG 0.435957 2.1:-6455869 None:intergenic
TCTATCGCCGCCTTTGACGC+CGG 0.437391 2.1:-6456121 None:intergenic
CAAATCGAAATCAACGGTTT+CGG 0.449596 2.1:+6456082 MS.gene45528:CDS
CTCTAACAACCGATCGAAGC+CGG 0.463112 2.1:-6456050 None:intergenic
ATTGACGATATGGAGGTTAC+CGG 0.464101 2.1:+6456393 MS.gene45528:CDS
GTTTGTCCTCACTGCCAAAA+CGG 0.475086 2.1:+6455716 MS.gene45528:CDS
TAGAAGAATTTCTTTGACTG+TGG 0.477614 2.1:+6456522 MS.gene45528:CDS
TTCGCCGGTGGTCGGAGAGC+CGG 0.489916 2.1:+6456439 MS.gene45528:CDS
GCCGGTGCCGGTGCAGACGC+CGG 0.490347 2.1:+6455908 MS.gene45528:CDS
TCCGACATGCTTGGTGGAAG+TGG 0.494594 2.1:-6456013 None:intergenic
AGAAACCGCCGGTGATTGAC+CGG 0.496031 2.1:-6455759 None:intergenic
CGCTTCTTCATCAATCTGAC+CGG 0.496970 2.1:-6456365 None:intergenic
ATCAACACCGCTACCGCCGC+CGG 0.500661 2.1:-6455927 None:intergenic
GGCGTTACGACGTCTGCGGC+GGG 0.502562 2.1:-6455822 None:intergenic
AAAAGGCGAACGGCTGCCGC+CGG 0.509087 2.1:-6455843 None:intergenic
TCCATCTCCGTGTAAACAAT+CGG 0.509848 2.1:-6456475 None:intergenic
TTCAATGCGAAATTCGTGTC+CGG 0.510386 2.1:+6456291 MS.gene45528:CDS
ACCGATTGTTTACACGGAGA+TGG 0.520025 2.1:+6456474 MS.gene45528:CDS
GGTGCCGGTGCAGACGCCGG+CGG 0.520180 2.1:+6455911 MS.gene45528:CDS
GGAGAATCCACCGGCGTCAA+AGG 0.524083 2.1:+6456111 MS.gene45528:CDS
CAGATTGATGAAGAAGCGGT+TGG 0.524412 2.1:+6456370 MS.gene45528:CDS
CCGGGAGGTGGATTTGCTGT+TGG 0.525108 2.1:+6456412 MS.gene45528:CDS
TTGGAAGATTCGCCGGTGGT+CGG 0.527536 2.1:+6456431 MS.gene45528:CDS
GAAGACACCACCGCCGGTGC+CGG 0.529621 2.1:+6455896 MS.gene45528:CDS
ATATGGAGGTTACCGGGAGG+TGG 0.532142 2.1:+6456400 MS.gene45528:CDS
ATTCCGATTGCATTCTCCCA+TGG 0.535454 2.1:+6456266 MS.gene45528:CDS
ATCGCCGCCTTTGACGCCGG+TGG 0.538439 2.1:-6456118 None:intergenic
TGCAAAGAACAATTCGAGCT+AGG 0.538605 2.1:+6456205 MS.gene45528:CDS
AAGCCATGGGAGAATGCAAT+CGG 0.539317 2.1:-6456269 None:intergenic
GGCGTCTGCACCGGCACCGG+CGG 0.539489 2.1:-6455906 None:intergenic
AATAGCTCCGACATGCTTGG+TGG 0.540581 2.1:-6456019 None:intergenic
TGGTGGAAGTGGACGTAGAT+CGG 0.541171 2.1:-6456002 None:intergenic
AGAAGAATTTCTTTGACTGT+GGG 0.543294 2.1:+6456523 MS.gene45528:CDS
GATTCACGCCGGTCAATCAC+CGG 0.546800 2.1:+6455751 MS.gene45528:CDS
GAATCGGTGATTTCAACTGT+TGG 0.552700 2.1:-6456151 None:intergenic
TCAGTTACCGATTGTTTACA+CGG 0.553870 2.1:+6456468 MS.gene45528:CDS
CGCCGCAGACGTCGTAACGC+CGG 0.555138 2.1:+6455824 MS.gene45528:CDS
AGTAATAGCTCCGACATGCT+TGG 0.555247 2.1:-6456022 None:intergenic
GAATTTCGCATTGAAAGCCA+TGG 0.556326 2.1:-6456283 None:intergenic
CGGTCAGATTGATGAAGAAG+CGG 0.556476 2.1:+6456366 MS.gene45528:CDS
CGCAGACGTCGTAACGCCGG+CGG 0.559927 2.1:+6455827 MS.gene45528:CDS
GAAATTCGTGTCCGGTGTGT+CGG 0.561109 2.1:+6456299 MS.gene45528:CDS
CGGCGTTACGACGTCTGCGG+CGG 0.562313 2.1:-6455823 None:intergenic
GCGGGAGGTTTGTCTTCTGG+AGG 0.564523 2.1:-6455804 None:intergenic
TTCTCTCAAATCGAAATCAA+CGG 0.566290 2.1:+6456076 MS.gene45528:CDS
GCACCAAAACGACGTCGTTG+AGG 0.569778 2.1:-6455648 None:intergenic
AATCAACGGTTTCGGAAGGT+CGG 0.570591 2.1:+6456090 MS.gene45528:CDS
CGGAAGGTCGGAGAATCCAC+CGG 0.582211 2.1:+6456102 MS.gene45528:CDS
CAAAACGACGTCGTTGAGGA+TGG 0.582320 2.1:-6455644 None:intergenic
TGGTGTCTTCTCTAGGTCCA+CGG 0.584451 2.1:-6455883 None:intergenic
CGGTAGCGGTGTTGATAGCG+AGG 0.586301 2.1:+6455934 MS.gene45528:CDS
TTTGCTGTTGGAAGATTCGC+CGG 0.595189 2.1:+6456424 MS.gene45528:CDS
ATTGTTTACACGGAGATGGA+TGG 0.596456 2.1:+6456478 MS.gene45528:CDS
AAGAAGAAGAAGCAGAACTT+GGG 0.600997 2.1:+6456663 MS.gene45528:CDS
TTGACGATATGGAGGTTACC+GGG 0.603171 2.1:+6456394 MS.gene45528:CDS
TCGAAATCAACGGTTTCGGA+AGG 0.605406 2.1:+6456086 MS.gene45528:CDS
CGCCGGCGTTACGACGTCTG+CGG 0.608354 2.1:-6455826 None:intergenic
CCAACAGCAAATCCACCTCC+CGG 0.611076 2.1:-6456412 None:intergenic
AATTTCGCATTGAAAGCCAT+GGG 0.617522 2.1:-6456282 None:intergenic
GTTACGACGTCTGCGGCGGG+AGG 0.618296 2.1:-6455819 None:intergenic
CTCACCGGCTCTCCGACCAC+CGG 0.618605 2.1:-6456443 None:intergenic
GAATCCACCGGCGTCAAAGG+CGG 0.619728 2.1:+6456114 MS.gene45528:CDS
AGCATGTCGGAGCTATTACT+CGG 0.620643 2.1:+6456025 MS.gene45528:CDS
AATCGGTAACTGAATCTCAC+CGG 0.623794 2.1:-6456458 None:intergenic
ATGGGAGAATGCAATCGGAA+TGG 0.625343 2.1:-6456264 None:intergenic
CCTAGAGAAGACACCACCGC+CGG 0.626450 2.1:+6455890 MS.gene45528:CDS
AATAGAGTTAGAGAGAGACG+TGG 0.627523 2.1:+6456550 MS.gene45528:CDS
CGGTTGGATTGACGATATGG+AGG 0.630916 2.1:+6456386 MS.gene45528:CDS
GGTAGCGGTGTTGATAGCGA+GGG 0.633690 2.1:+6455935 MS.gene45528:CDS
GGTAACTCGTGCCGACACAC+CGG 0.638241 2.1:-6456310 None:intergenic
TCCACTTCCACCAAGCATGT+CGG 0.655861 2.1:+6456012 MS.gene45528:CDS
AATCCTGTAATCGTTCTCCG+TGG 0.656883 2.1:+6455866 MS.gene45528:CDS
TCTGGAGGACAGAATACCGT+CGG 0.669391 2.1:-6455789 None:intergenic
TCACGCCGGTCAATCACCGG+CGG 0.670342 2.1:+6455754 MS.gene45528:CDS
TCGGCGAAAAGAGAAACCGC+CGG 0.670628 2.1:-6455770 None:intergenic
GAATGGTAAAGATGATTACA+CGG 0.678082 2.1:-6456247 None:intergenic
GTAGCGGTGTTGATAGCGAG+GGG 0.678382 2.1:+6455936 MS.gene45528:CDS
TTATACTATGAAGACAGTAA+CGG 0.680959 2.1:+6455977 MS.gene45528:CDS
TTACAGGATTAAAAGGCGAA+CGG 0.681942 2.1:-6455853 None:intergenic
GCTGTTGGAAGATTCGCCGG+TGG 0.685393 2.1:+6456427 MS.gene45528:CDS
TTCGTCGAAGAGATTCACGC+CGG 0.729026 2.1:+6455740 MS.gene45528:CDS
ACGATATGGAGGTTACCGGG+AGG 0.773215 2.1:+6456397 MS.gene45528:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTTTTTATCGTTTTTT+GGG + chr2.1:6456590-6456609 MS.gene45528:CDS 15.0%
!!! CGAAATTTTTTATCGTTTTT+TGG + chr2.1:6456589-6456608 MS.gene45528:CDS 20.0%
! TTATACTATGAAGACAGTAA+CGG + chr2.1:6455977-6455996 MS.gene45528:CDS 25.0%
AGAACGATTACAGGATTAAA+AGG - chr2.1:6455863-6455882 None:intergenic 30.0%
AGAAGAATTTCTTTGACTGT+GGG + chr2.1:6456523-6456542 MS.gene45528:CDS 30.0%
GAATGGTAAAGATGATTACA+CGG - chr2.1:6456250-6456269 None:intergenic 30.0%
TAGAAGAATTTCTTTGACTG+TGG + chr2.1:6456522-6456541 MS.gene45528:CDS 30.0%
TTCTCTCAAATCGAAATCAA+CGG + chr2.1:6456076-6456095 MS.gene45528:CDS 30.0%
! AACTTAAATCGTAGCTTTTC+TGG + chr2.1:6456619-6456638 MS.gene45528:CDS 30.0%
AAGAAGAAGAAGCAGAACTT+GGG + chr2.1:6456663-6456682 MS.gene45528:CDS 35.0%
AATTTCGCATTGAAAGCCAT+GGG - chr2.1:6456285-6456304 None:intergenic 35.0%
CAAATCGAAATCAACGGTTT+CGG + chr2.1:6456082-6456101 MS.gene45528:CDS 35.0%
TTACAGGATTAAAAGGCGAA+CGG - chr2.1:6455856-6455875 None:intergenic 35.0%
TTCGGATTCGATTTCTGAAT+CGG - chr2.1:6456170-6456189 None:intergenic 35.0%
! TCAGTTACCGATTGTTTACA+CGG + chr2.1:6456468-6456487 MS.gene45528:CDS 35.0%
AATAGAGTTAGAGAGAGACG+TGG + chr2.1:6456550-6456569 MS.gene45528:CDS 40.0%
AATCGGTAACTGAATCTCAC+CGG - chr2.1:6456461-6456480 None:intergenic 40.0%
ATTGACGATATGGAGGTTAC+CGG + chr2.1:6456393-6456412 MS.gene45528:CDS 40.0%
ATTGTTTACACGGAGATGGA+TGG + chr2.1:6456478-6456497 MS.gene45528:CDS 40.0%
CAAGAAGAAGAAGCAGAACT+TGG + chr2.1:6456662-6456681 MS.gene45528:CDS 40.0%
GAATCGGTGATTTCAACTGT+TGG - chr2.1:6456154-6456173 None:intergenic 40.0%
GAATTTCGCATTGAAAGCCA+TGG - chr2.1:6456286-6456305 None:intergenic 40.0%
GCAAACTGCACAGTGAATTT+CGG - chr2.1:6456188-6456207 None:intergenic 40.0%
TCCATCTCCGTGTAAACAAT+CGG - chr2.1:6456478-6456497 None:intergenic 40.0%
TGCAAAGAACAATTCGAGCT+AGG + chr2.1:6456205-6456224 MS.gene45528:CDS 40.0%
TTAGAGAGAGACGTGGAATT+CGG + chr2.1:6456557-6456576 MS.gene45528:CDS 40.0%
TTCAATGCGAAATTCGTGTC+CGG + chr2.1:6456291-6456310 MS.gene45528:CDS 40.0%
AAGCCATGGGAGAATGCAAT+CGG - chr2.1:6456272-6456291 None:intergenic 45.0%
AAGCGGTTGGATTGACGATA+TGG + chr2.1:6456383-6456402 MS.gene45528:CDS 45.0%
AATCAACGGTTTCGGAAGGT+CGG + chr2.1:6456090-6456109 MS.gene45528:CDS 45.0%
AATCCTGTAATCGTTCTCCG+TGG + chr2.1:6455866-6455885 MS.gene45528:CDS 45.0%
ACCGATTGTTTACACGGAGA+TGG + chr2.1:6456474-6456493 MS.gene45528:CDS 45.0%
AGCATGTCGGAGCTATTACT+CGG + chr2.1:6456025-6456044 MS.gene45528:CDS 45.0%
AGTAATAGCTCCGACATGCT+TGG - chr2.1:6456025-6456044 None:intergenic 45.0%
ATGGGAGAATGCAATCGGAA+TGG - chr2.1:6456267-6456286 None:intergenic 45.0%
ATTCCGATTGCATTCTCCCA+TGG + chr2.1:6456266-6456285 MS.gene45528:CDS 45.0%
CAGATTGATGAAGAAGCGGT+TGG + chr2.1:6456370-6456389 MS.gene45528:CDS 45.0%
CGCTTCTTCATCAATCTGAC+CGG - chr2.1:6456368-6456387 None:intergenic 45.0%
CGGTCAGATTGATGAAGAAG+CGG + chr2.1:6456366-6456385 MS.gene45528:CDS 45.0%
GCGTCTGAGAGTAGAATTTC+CGG + chr2.1:6456346-6456365 MS.gene45528:CDS 45.0%
GTTTGTCCTCACTGCCAAAA+CGG + chr2.1:6455716-6455735 MS.gene45528:CDS 45.0%
TCGAAATCAACGGTTTCGGA+AGG + chr2.1:6456086-6456105 MS.gene45528:CDS 45.0%
TTGACGATATGGAGGTTACC+GGG + chr2.1:6456394-6456413 MS.gene45528:CDS 45.0%
! GAGGACAAACGACGTTGTTT+TGG - chr2.1:6455706-6455725 None:intergenic 45.0%
! TCTCTTCGACGAATCCGTTT+TGG - chr2.1:6455733-6455752 None:intergenic 45.0%
!! ACTCTCAGACGCGTTTTGAT+CGG - chr2.1:6456338-6456357 None:intergenic 45.0%
!! TTTGCTGTTGGAAGATTCGC+CGG + chr2.1:6456424-6456443 MS.gene45528:CDS 45.0%
AATAGCTCCGACATGCTTGG+TGG - chr2.1:6456022-6456041 None:intergenic 50.0%
CAAAACGACGTCGTTGAGGA+TGG - chr2.1:6455647-6455666 None:intergenic 50.0%
CGGTTGGATTGACGATATGG+AGG + chr2.1:6456386-6456405 MS.gene45528:CDS 50.0%
CTCTAACAACCGATCGAAGC+CGG - chr2.1:6456053-6456072 None:intergenic 50.0%
GAAATTCGTGTCCGGTGTGT+CGG + chr2.1:6456299-6456318 MS.gene45528:CDS 50.0%
TCCACTTCCACCAAGCATGT+CGG + chr2.1:6456012-6456031 MS.gene45528:CDS 50.0%
TCGGAGCTATTACTCGGTTC+CGG + chr2.1:6456031-6456050 MS.gene45528:CDS 50.0%
TCTGGAGGACAGAATACCGT+CGG - chr2.1:6455792-6455811 None:intergenic 50.0%
TGGTGGAAGTGGACGTAGAT+CGG - chr2.1:6456005-6456024 None:intergenic 50.0%
TGGTGTCTTCTCTAGGTCCA+CGG - chr2.1:6455886-6455905 None:intergenic 50.0%
TTCGTCGAAGAGATTCACGC+CGG + chr2.1:6455740-6455759 MS.gene45528:CDS 50.0%
! CATCCTCAACGACGTCGTTT+TGG + chr2.1:6455645-6455664 MS.gene45528:CDS 50.0%
!! ACGAATCCGTTTTGGCAGTG+AGG - chr2.1:6455725-6455744 None:intergenic 50.0%
!! TCAGACGCGTTTTGATCGGA+CGG - chr2.1:6456334-6456353 None:intergenic 50.0%
ACGATATGGAGGTTACCGGG+AGG + chr2.1:6456397-6456416 MS.gene45528:CDS 55.0%
AGAAACCGCCGGTGATTGAC+CGG - chr2.1:6455762-6455781 None:intergenic 55.0%
ATATGGAGGTTACCGGGAGG+TGG + chr2.1:6456400-6456419 MS.gene45528:CDS 55.0%
CCAACAGCAAATCCACCTCC+CGG - chr2.1:6456415-6456434 None:intergenic 55.0%
GATTCACGCCGGTCAATCAC+CGG + chr2.1:6455751-6455770 MS.gene45528:CDS 55.0%
GCACCAAAACGACGTCGTTG+AGG - chr2.1:6455651-6455670 None:intergenic 55.0%
GGTCCACGGAGAACGATTAC+AGG - chr2.1:6455872-6455891 None:intergenic 55.0%
TACTCGGTTCCGGCTTCGAT+CGG + chr2.1:6456041-6456060 MS.gene45528:CDS 55.0%
TCGGCGAAAAGAGAAACCGC+CGG - chr2.1:6455773-6455792 None:intergenic 55.0%
TTGGAAGATTCGCCGGTGGT+CGG + chr2.1:6456431-6456450 MS.gene45528:CDS 55.0%
! GCGGTTTCTCTTTTCGCCGA+CGG + chr2.1:6455773-6455792 MS.gene45528:CDS 55.0%
! TCCGACATGCTTGGTGGAAG+TGG - chr2.1:6456016-6456035 None:intergenic 55.0%
!! GGTAGCGGTGTTGATAGCGA+GGG + chr2.1:6455935-6455954 MS.gene45528:CDS 55.0%
!! GTAGCGGTGTTGATAGCGAG+GGG + chr2.1:6455936-6455955 MS.gene45528:CDS 55.0%
CCTAGAGAAGACACCACCGC+CGG + chr2.1:6455890-6455909 MS.gene45528:CDS 60.0%
CGGAAGGTCGGAGAATCCAC+CGG + chr2.1:6456102-6456121 MS.gene45528:CDS 60.0%
GAATCCACCGGCGTCAAAGG+CGG + chr2.1:6456114-6456133 MS.gene45528:CDS 60.0%
GCGGGAGGTTTGTCTTCTGG+AGG - chr2.1:6455807-6455826 None:intergenic 60.0%
GCTGTTGGAAGATTCGCCGG+TGG + chr2.1:6456427-6456446 MS.gene45528:CDS 60.0%
GGAGAATCCACCGGCGTCAA+AGG + chr2.1:6456111-6456130 MS.gene45528:CDS 60.0%
TCTATCGCCGCCTTTGACGC+CGG - chr2.1:6456124-6456143 None:intergenic 60.0%
! GGTAACTCGTGCCGACACAC+CGG - chr2.1:6456313-6456332 None:intergenic 60.0%
!! CCGGGAGGTGGATTTGCTGT+TGG + chr2.1:6456412-6456431 MS.gene45528:CDS 60.0%
!! CGGTAGCGGTGTTGATAGCG+AGG + chr2.1:6455934-6455953 MS.gene45528:CDS 60.0%
AAAAGGCGAACGGCTGCCGC+CGG - chr2.1:6455846-6455865 None:intergenic 65.0%
ATCAACACCGCTACCGCCGC+CGG - chr2.1:6455930-6455949 None:intergenic 65.0%
CCGGCGGTGGTGTCTTCTCT+AGG - chr2.1:6455893-6455912 None:intergenic 65.0%
GCGGCGGGAGGTTTGTCTTC+TGG - chr2.1:6455810-6455829 None:intergenic 65.0%
TCACGCCGGTCAATCACCGG+CGG + chr2.1:6455754-6455773 MS.gene45528:CDS 65.0%
ATCGCCGCCTTTGACGCCGG+TGG - chr2.1:6456121-6456140 None:intergenic 70.0%
CGCAGACGTCGTAACGCCGG+CGG + chr2.1:6455827-6455846 MS.gene45528:CDS 70.0%
CGCCGCAGACGTCGTAACGC+CGG + chr2.1:6455824-6455843 MS.gene45528:CDS 70.0%
CGCCGGCGTTACGACGTCTG+CGG - chr2.1:6455829-6455848 None:intergenic 70.0%
CGGCGTTACGACGTCTGCGG+CGG - chr2.1:6455826-6455845 None:intergenic 70.0%
GAAGACACCACCGCCGGTGC+CGG + chr2.1:6455896-6455915 MS.gene45528:CDS 70.0%
GGCGTTACGACGTCTGCGGC+GGG - chr2.1:6455825-6455844 None:intergenic 70.0%
GTTACGACGTCTGCGGCGGG+AGG - chr2.1:6455822-6455841 None:intergenic 70.0%
TTCGCCGGTGGTCGGAGAGC+CGG + chr2.1:6456439-6456458 MS.gene45528:CDS 70.0%
!! CTCACCGGCTCTCCGACCAC+CGG - chr2.1:6456446-6456465 None:intergenic 70.0%
! ACCGCCGCCGGCGTCTGCAC+CGG - chr2.1:6455918-6455937 None:intergenic 80.0%
! GCAGACGCCGGCGGCGGTAG+CGG + chr2.1:6455920-6455939 MS.gene45528:CDS 80.0%
!! GCCGGCGTCTGCACCGGCAC+CGG - chr2.1:6455912-6455931 None:intergenic 80.0%
!! GGCGTCTGCACCGGCACCGG+CGG - chr2.1:6455909-6455928 None:intergenic 80.0%
!!! GCCGGTGCCGGTGCAGACGC+CGG + chr2.1:6455908-6455927 MS.gene45528:CDS 80.0%
!!! GGTGCCGGTGCAGACGCCGG+CGG + chr2.1:6455911-6455930 MS.gene45528:CDS 80.0%
!!! GTCTGCACCGGCACCGGCGG+TGG - chr2.1:6455906-6455925 None:intergenic 80.0%
! GCCGGTGCAGACGCCGGCGG+CGG + chr2.1:6455914-6455933 MS.gene45528:CDS 85.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 6455623 6456699 6455623 ID=MS.gene45528
chr2.1 mRNA 6455623 6456699 6455623 ID=MS.gene45528.t1;Parent=MS.gene45528
chr2.1 exon 6455623 6456699 6455623 ID=MS.gene45528.t1.exon1;Parent=MS.gene45528.t1
chr2.1 CDS 6455623 6456699 6455623 ID=cds.MS.gene45528.t1;Parent=MS.gene45528.t1
Gene Sequence

>MS.gene45528

ATGAACTCGGAAACTGAAACACCATCCTCAACGACGTCGTTTTGGTGCTACAGCTGCACGCGTTTCATTCACATCTTGAACCAAAACAACGTCGTTTGTCCTCACTGCCAAAACGGATTCGTCGAAGAGATTCACGCCGGTCAATCACCGGCGGTTTCTCTTTTCGCCGACGGTATTCTGTCCTCCAGAAGACAAACCTCCCGCCGCAGACGTCGTAACGCCGGCGGCAGCCGTTCGCCTTTTAATCCTGTAATCGTTCTCCGTGGACCTAGAGAAGACACCACCGCCGGTGCCGGTGCAGACGCCGGCGGCGGTAGCGGTGTTGATAGCGAGGGGAATACAAGTAGTTTTGAATTATACTATGAAGACAGTAACGGTACCGATCTACGTCCACTTCCACCAAGCATGTCGGAGCTATTACTCGGTTCCGGCTTCGATCGGTTGTTAGAGCAATTCTCTCAAATCGAAATCAACGGTTTCGGAAGGTCGGAGAATCCACCGGCGTCAAAGGCGGCGATAGAGTCAATACCAACAGTTGAAATCACCGATTCAGAAATCGAATCCGAAATTCACTGTGCAGTTTGCAAAGAACAATTCGAGCTAGGTTCTGAAGCGCGTAAGATGCCGTGTAATCATCTTTACCATTCCGATTGCATTCTCCCATGGCTTTCAATGCGAAATTCGTGTCCGGTGTGTCGGCACGAGTTACCGTCCGATCAAAACGCGTCTGAGAGTAGAATTTCCGGTCAGATTGATGAAGAAGCGGTTGGATTGACGATATGGAGGTTACCGGGAGGTGGATTTGCTGTTGGAAGATTCGCCGGTGGTCGGAGAGCCGGTGAGATTCAGTTACCGATTGTTTACACGGAGATGGATGGTAACAATTCAAACGAAGATTCTAGAAGAATTTCTTTGACTGTGGGAAGTAATAGAGTTAGAGAGAGACGTGGAATTCGGAGAATTTTTCGAAATTTTTTATCGTTTTTTGGGAATGTCAACTTAAATCGTAGCTTTTCTGGTTCGTTGTTTAGTAGAAGCTCAAGAAGAAGAAGCAGAACTTGGGTTACAGAAGAATAA
Protein sequence

>MS.gene45528.t1

MNSETETPSSTTSFWCYSCTRFIHILNQNNVVCPHCQNGFVEEIHAGQSPAVSLFADGILSSRRQTSRRRRRNAGGSRSPFNPVIVLRGPREDTTAGAGADAGGGSGVDSEGNTSSFELYYEDSNGTDLRPLPPSMSELLLGSGFDRLLEQFSQIEINGFGRSENPPASKAAIESIPTVEITDSEIESEIHCAVCKEQFELGSEARKMPCNHLYHSDCILPWLSMRNSCPVCRHELPSDQNASESRISGQIDEEAVGLTIWRLPGGGFAVGRFAGGRRAGEIQLPIVYTEMDGNNSNEDSRRISLTVGSNRVRERRGIRRIFRNFLSFFGNVNLNRSFSGSLFSRSSRRRSRTWVTEE