AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45530.t1 MTR_5g032760 79.755 163 23 2 61 213 2 164 3.30e-52 166
MS.gene45530.t1 MTR_2g097650 100.000 67 0 0 147 213 3 69 1.91e-40 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGGTGGACTAGTGCTTT+TGG 0.225862 2.1:+6437035 MS.gene45530:CDS
AGCATCGCGGGTTATAGTTT+GGG 0.348033 2.1:-6435826 None:intergenic
GAGCATCGCGGGTTATAGTT+TGG 0.349832 2.1:-6435827 None:intergenic
TTCTTCTCTCCAGGGATTGA+GGG 0.363361 2.1:+6434020 MS.gene45530:intron
CCGCGATGCTCGCACAATTA+CGG 0.385032 2.1:+6435839 MS.gene45530:CDS
GGTAATGGAGGAGGCGGTGG+TGG 0.419598 2.1:+6437006 MS.gene45530:CDS
AAGAAGAGTGAGAGGGTCGA+TGG 0.420559 2.1:-6434087 None:intergenic
ACGAATACTTAGAGCATTGT+CGG 0.422424 2.1:+6433871 MS.gene45530:CDS
CGTCTCATCGCAACAATGTT+CGG 0.426602 2.1:-6434586 None:intergenic
TCGTCGCGAGGTGGCAATCC+AGG 0.433703 2.1:-6436970 None:intergenic
AGACTTGATGACAATGGGCC+TGG 0.438601 2.1:+6436952 MS.gene45530:CDS
TTTCTTCTCTCCAGGGATTG+AGG 0.439821 2.1:+6434019 MS.gene45530:intron
CGTAATTGTGCGAGCATCGC+GGG 0.445247 2.1:-6435838 None:intergenic
AAGAGTGAGAGGGTCGATGG+AGG 0.446007 2.1:-6434084 None:intergenic
CATCGCAACAATGTTCGGTC+TGG 0.462516 2.1:-6434581 None:intergenic
GGTGGTAATGGAGGAGGCGG+TGG 0.465666 2.1:+6437003 MS.gene45530:CDS
ATTAAAATGTGACGCTGCTA+TGG 0.476209 2.1:+6434672 MS.gene45530:CDS
AGAGTGAGAGGGTCGATGGA+GGG 0.480080 2.1:-6434083 None:intergenic
TGGTCATCCCGACTGGATAC+TGG 0.486913 2.1:+6433936 MS.gene45530:CDS
GATGATGGCAATGGTGGTAA+TGG 0.487379 2.1:+6436991 MS.gene45530:CDS
AACTGCTCAGTCTGAAAACC+AGG 0.490033 2.1:-6433909 None:intergenic
AGATTAGACTTGATGACAAT+GGG 0.494836 2.1:+6436947 MS.gene45530:CDS
TTGCCACCTCGCGACGATGA+TGG 0.502965 2.1:+6436976 MS.gene45530:CDS
TAGAGAAGAAGAAGAGTGAG+AGG 0.515642 2.1:-6434095 None:intergenic
AACGAACAGAGAGGGAGAGA+TGG 0.517852 2.1:-6434123 None:intergenic
CTGAAAACCAGGGATTAAAC+CGG 0.521219 2.1:-6433898 None:intergenic
CAATGTCTTTAAGAATGTCT+AGG 0.523559 2.1:-6437071 None:intergenic
GAGGGAGTCGCGAGCAGTGA+AGG 0.545102 2.1:-6434065 None:intergenic
AAGATTAGACTTGATGACAA+TGG 0.549867 2.1:+6436946 MS.gene45530:CDS
CGCACAATTACGGTTACTCC+TGG 0.560145 2.1:+6435849 MS.gene45530:CDS
GGCAATGGTGGTAATGGAGG+AGG 0.565209 2.1:+6436997 MS.gene45530:CDS
AACCGGACCAGTATCCAGTC+GGG 0.569992 2.1:-6433943 None:intergenic
TCTTCTCTCCAGGGATTGAG+GGG 0.578770 2.1:+6434021 MS.gene45530:intron
CCTCGCGACGATGATGGCAA+TGG 0.603587 2.1:+6436982 MS.gene45530:CDS
CGCGACGATGATGGCAATGG+TGG 0.606298 2.1:+6436985 MS.gene45530:CDS
AATGGTGGTAATGGAGGAGG+CGG 0.609101 2.1:+6437000 MS.gene45530:CDS
GATGGCAATGGTGGTAATGG+AGG 0.610367 2.1:+6436994 MS.gene45530:CDS
GTCGCGAGCAGTGAAGGTGG+CGG 0.613568 2.1:-6434059 None:intergenic
GGAGTCGCGAGCAGTGAAGG+TGG 0.620605 2.1:-6434062 None:intergenic
GAACCGGACCAGTATCCAGT+CGG 0.627395 2.1:-6433944 None:intergenic
TTGCCATCATCGTCGCGAGG+TGG 0.640575 2.1:-6436979 None:intergenic
CCGTAATTGTGCGAGCATCG+CGG 0.652509 2.1:-6435839 None:intergenic
TCTGTAGTAACGAACAGAGA+GGG 0.654766 2.1:-6434131 None:intergenic
AGAGAAGAAGAAGAGTGAGA+GGG 0.663417 2.1:-6434094 None:intergenic
ACTGCTCAGTCTGAAAACCA+GGG 0.716458 2.1:-6433908 None:intergenic
CCATTGCCATCATCGTCGCG+AGG 0.726947 2.1:-6436982 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGATTAAAAAATTGAA+GGG - chr2.1:6434214-6434233 None:intergenic 10.0%
!! ATATGAATATTTCATAATTA+AGG + chr2.1:6436161-6436180 MS.gene45530:intron 10.0%
!!! GATATTTTTATTTTTTTTGT+TGG + chr2.1:6434505-6434524 MS.gene45530:intron 10.0%
!! AAAAAAACTAATAAGATTGT+AGG - chr2.1:6436512-6436531 None:intergenic 15.0%
!! ACAGAATAAAAAATCAATTA+AGG - chr2.1:6436850-6436869 None:intergenic 15.0%
!! ATACCATATAACATAAAAAA+AGG + chr2.1:6434610-6434629 MS.gene45530:CDS 15.0%
!! CAATAAGATTAAAAAATTGA+AGG - chr2.1:6434215-6434234 None:intergenic 15.0%
!! CAGAATAAAAAATCAATTAA+GGG - chr2.1:6436849-6436868 None:intergenic 15.0%
!! TACCATATAACATAAAAAAA+GGG + chr2.1:6434611-6434630 MS.gene45530:CDS 15.0%
!! TAGTTTATATAAATCATTCA+TGG + chr2.1:6434483-6434502 MS.gene45530:intron 15.0%
!! TATATCATAATCCAATATAA+CGG + chr2.1:6435027-6435046 MS.gene45530:intron 15.0%
!!! ATCAATTAACAGTTTTATTT+TGG - chr2.1:6434879-6434898 None:intergenic 15.0%
!!! ATTTTTTAATCTTATTGTGT+TGG + chr2.1:6434218-6434237 MS.gene45530:intron 15.0%
!!! TAATTTTCCTTAAAGAAAAA+AGG + chr2.1:6433796-6433815 MS.gene45530:intron 15.0%
!! ATTATTATGTTAGATTTCTG+AGG + chr2.1:6433755-6433774 MS.gene45530:intron 20.0%
!! CAACAACAGTAAATTTATAA+AGG + chr2.1:6436401-6436420 MS.gene45530:intron 20.0%
!! TATATTTGTATACATAGACA+AGG - chr2.1:6436893-6436912 None:intergenic 20.0%
!! TGGTTAATATGAAAATTACA+AGG + chr2.1:6433996-6434015 MS.gene45530:intron 20.0%
!! TTAAAAAATTGAAGGGAAAA+AGG - chr2.1:6434207-6434226 None:intergenic 20.0%
!! TTAAAGGAAAATAACAAGAA+TGG - chr2.1:6435338-6435357 None:intergenic 20.0%
!!! AATAATTTTTATCTTGCAAG+GGG + chr2.1:6434900-6434919 MS.gene45530:intron 20.0%
!!! AATTGCTAATTTTGTAGAAA+AGG - chr2.1:6436803-6436822 None:intergenic 20.0%
!!! ATGGAAATTTTGGATTTTAT+TGG + chr2.1:6435662-6435681 MS.gene45530:intron 20.0%
!!! ATTGTATCCTTTTTTCTTTA+AGG - chr2.1:6433806-6433825 None:intergenic 20.0%
!!! ATTTTTTATAGGATGGAATA+TGG + chr2.1:6435594-6435613 MS.gene45530:intron 20.0%
!!! GAATAATTTTTATCTTGCAA+GGG + chr2.1:6434899-6434918 MS.gene45530:intron 20.0%
!!! TAAAGGATTTTGATATTAGA+AGG + chr2.1:6436418-6436437 MS.gene45530:intron 20.0%
!!! TATGGTATGGTTTTTTATTT+TGG + chr2.1:6434690-6434709 MS.gene45530:intron 20.0%
!!! TGAATAATTTTTATCTTGCA+AGG + chr2.1:6434898-6434917 MS.gene45530:intron 20.0%
!!! TGTAATTTTCATATTAACCA+AGG - chr2.1:6433996-6434015 None:intergenic 20.0%
! AATCCAAAATTTCCATGATT+CGG - chr2.1:6435658-6435677 None:intergenic 25.0%
! ACTAGTATAGAAAATGGAAT+TGG - chr2.1:6436013-6436032 None:intergenic 25.0%
! ACTTATCCAAAATTGTTTGT+TGG + chr2.1:6435707-6435726 MS.gene45530:intron 25.0%
! ATAGCATTTGTCATAATATG+TGG + chr2.1:6436736-6436755 MS.gene45530:intron 25.0%
! ATGATGACTAGTATAGAAAA+TGG - chr2.1:6436019-6436038 None:intergenic 25.0%
! ATGCTAAATGATATTCTTGT+TGG + chr2.1:6434786-6434805 MS.gene45530:intron 25.0%
! GAAAAAACAACGTACAATTA+GGG - chr2.1:6434165-6434184 None:intergenic 25.0%
! TGAAAAAACAACGTACAATT+AGG - chr2.1:6434166-6434185 None:intergenic 25.0%
! TTAACCAAGGTTATAAAAAC+CGG - chr2.1:6433983-6434002 None:intergenic 25.0%
!! ATCTATTTTCCTATTCTATC+TGG - chr2.1:6435150-6435169 None:intergenic 25.0%
!! CATTTGAAGTGCATAAATAA+TGG + chr2.1:6436222-6436241 MS.gene45530:intron 25.0%
!! TCTATTTTCCTATTCTATCT+GGG - chr2.1:6435149-6435168 None:intergenic 25.0%
!!! AACAGGGGATTTTTATTTTT+TGG + chr2.1:6436482-6436501 MS.gene45530:intron 25.0%
!!! TCCAACATCATATCATTTTT+TGG - chr2.1:6435902-6435921 None:intergenic 25.0%
!!! TGCCCTTTTTTTATGTTATA+TGG - chr2.1:6434616-6434635 None:intergenic 25.0%
!!! TGTATTGTTTGTTTTACACT+GGG + chr2.1:6435950-6435969 MS.gene45530:intron 25.0%
!!! TTGTATTGTTTGTTTTACAC+TGG + chr2.1:6435949-6435968 MS.gene45530:intron 25.0%
!!! TTTCTGTAGGCATTTTTTAT+AGG + chr2.1:6435583-6435602 MS.gene45530:intron 25.0%
AAATCTGTCTACTTAACTGA+AGG + chr2.1:6436458-6436477 MS.gene45530:intron 30.0%
AACGTACAATTAGGGTTTAT+GGG - chr2.1:6434157-6434176 None:intergenic 30.0%
AAGTTTCATATGTATGAGAG+TGG - chr2.1:6435497-6435516 None:intergenic 30.0%
AATCTGTCTACTTAACTGAA+GGG + chr2.1:6436459-6436478 MS.gene45530:intron 30.0%
AATGCCTACAGAAAAATCAA+TGG - chr2.1:6435577-6435596 None:intergenic 30.0%
ACAAAACACTATCTACAGAA+GGG - chr2.1:6436562-6436581 None:intergenic 30.0%
ACATGTAACATTCTGTAGAA+AGG - chr2.1:6435790-6435809 None:intergenic 30.0%
ACCTTACCAACTTATCAAAA+AGG - chr2.1:6434855-6434874 None:intergenic 30.0%
AGGTAGCATTGTTTAACATT+TGG + chr2.1:6434335-6434354 MS.gene45530:intron 30.0%
ATCAGGTTTCTTCTATAGTA+AGG - chr2.1:6435120-6435139 None:intergenic 30.0%
ATGTAACATTCTGTAGAAAG+GGG - chr2.1:6435788-6435807 None:intergenic 30.0%
CATGTAACATTCTGTAGAAA+GGG - chr2.1:6435789-6435808 None:intergenic 30.0%
CATTTGTCATAATATGTGGA+CGG + chr2.1:6436740-6436759 MS.gene45530:intron 30.0%
CTAAATCAATATCAAAGCGT+AGG + chr2.1:6436119-6436138 MS.gene45530:intron 30.0%
CTATCAGAAACATATCTGAA+TGG - chr2.1:6435233-6435252 None:intergenic 30.0%
GATATAAAATCCTGCCATAT+TGG - chr2.1:6435013-6435032 None:intergenic 30.0%
GCCAAAAAATGATATGATGT+TGG + chr2.1:6435898-6435917 MS.gene45530:intron 30.0%
GGAAAAAGGAAAATATATCG+AGG - chr2.1:6434193-6434212 None:intergenic 30.0%
TACAAAACACTATCTACAGA+AGG - chr2.1:6436563-6436582 None:intergenic 30.0%
TAGGATTCAACAGAAGTTAA+AGG - chr2.1:6435354-6435373 None:intergenic 30.0%
TCAACTAAAAATCTTGTACC+TGG - chr2.1:6434426-6434445 None:intergenic 30.0%
TCAATAAAGACACTGATGTA+TGG - chr2.1:6436094-6436113 None:intergenic 30.0%
TGCATAAATAATGGCTTTCT+TGG + chr2.1:6436231-6436250 MS.gene45530:intron 30.0%
TGTTTGCTTTCTTTATGCTA+TGG + chr2.1:6434724-6434743 MS.gene45530:intron 30.0%
! AAATGAATAGAGTTGAGTTG+TGG - chr2.1:6436779-6436798 None:intergenic 30.0%
! AAGATTAGACTTGATGACAA+TGG + chr2.1:6436946-6436965 MS.gene45530:CDS 30.0%
! AATCCGAATCATGGAAATTT+TGG + chr2.1:6435652-6435671 MS.gene45530:intron 30.0%
! AGATTAGACTTGATGACAAT+GGG + chr2.1:6436947-6436966 MS.gene45530:CDS 30.0%
! ATCAGTCCTTTTTGATAAGT+TGG + chr2.1:6434846-6434865 MS.gene45530:intron 30.0%
! ATTATGTTAGATTTCTGAGG+TGG + chr2.1:6433758-6433777 MS.gene45530:intron 30.0%
! CAATGTCTTTAAGAATGTCT+AGG - chr2.1:6437074-6437093 None:intergenic 30.0%
! CATTCAGATATGTTTCTGAT+AGG + chr2.1:6435231-6435250 MS.gene45530:intron 30.0%
! GTTTGTTTGATGGTTTCTTA+TGG + chr2.1:6436639-6436658 MS.gene45530:intron 30.0%
!! ATCTTTTTGTCTGTCATTTC+AGG - chr2.1:6436931-6436950 None:intergenic 30.0%
!!! TCCTTTTTGATAAGTTGGTA+AGG + chr2.1:6434851-6434870 MS.gene45530:intron 30.0%
!!! TGAGATAGTTTTAGCAACTT+AGG + chr2.1:6434443-6434462 MS.gene45530:intron 30.0%
!!! TGTAGGCATTTTTTATAGGA+TGG + chr2.1:6435587-6435606 MS.gene45530:intron 30.0%
!!! TGTTCCATTGATTTTTCTGT+AGG + chr2.1:6435570-6435589 MS.gene45530:intron 30.0%
AAACATATCTGAATGGTCAC+TGG - chr2.1:6435226-6435245 None:intergenic 35.0%
AACCATCAAACAAACACACT+TGG - chr2.1:6436634-6436653 None:intergenic 35.0%
AACTGAAACGAAACCATTCT+AGG - chr2.1:6436594-6436613 None:intergenic 35.0%
ACAAGAGGCAATGAAGAAAT+TGG - chr2.1:6435473-6435492 None:intergenic 35.0%
ACTAAAAATCTTGTACCTGG+CGG - chr2.1:6434423-6434442 None:intergenic 35.0%
ACTAGGATGCATGATTGTAA+AGG - chr2.1:6436264-6436283 None:intergenic 35.0%
ATCGAAAACCCAGATAGAAT+AGG + chr2.1:6435138-6435157 MS.gene45530:intron 35.0%
ATTAAAATGTGACGCTGCTA+TGG + chr2.1:6434672-6434691 MS.gene45530:CDS 35.0%
CAACGTACAATTAGGGTTTA+TGG - chr2.1:6434158-6434177 None:intergenic 35.0%
CAATGCAAAGAAGCTAGAAA+AGG + chr2.1:6436363-6436382 MS.gene45530:intron 35.0%
CGATATTCTGATGTCTTGTA+TGG + chr2.1:6435734-6435753 MS.gene45530:intron 35.0%
CTTACTTTCAATCCGAATCA+TGG + chr2.1:6435643-6435662 MS.gene45530:intron 35.0%
GATCAAGATAGGAAAGTGAT+AGG - chr2.1:6435373-6435392 None:intergenic 35.0%
GCAATGAAGAAATTGGAACT+AGG - chr2.1:6435466-6435485 None:intergenic 35.0%
GCTTTCTTTATGCTATGGTA+TGG + chr2.1:6434729-6434748 MS.gene45530:intron 35.0%
TCATATTCGGATGTCTTGTA+TGG + chr2.1:6435760-6435779 MS.gene45530:intron 35.0%
TCTTGTATGGTGCTCATATT+CGG + chr2.1:6435747-6435766 MS.gene45530:intron 35.0%
TTCAGATAGAAGAGAAACAG+TGG - chr2.1:6435424-6435443 None:intergenic 35.0%
TTGTAGTCTGTTTCCTAGAA+TGG + chr2.1:6436578-6436597 MS.gene45530:intron 35.0%
TTGTGCAAAAGGTCTATGTT+AGG + chr2.1:6435269-6435288 MS.gene45530:intron 35.0%
! ACATAGACCTTTTGCACAAA+AGG - chr2.1:6435268-6435287 None:intergenic 35.0%
! CGTAGACCAACAAACAATTT+TGG - chr2.1:6435716-6435735 None:intergenic 35.0%
! CTTTTGACCTTTTGTGCAAA+AGG + chr2.1:6435258-6435277 MS.gene45530:intron 35.0%
! CTTTTGCACAAAAGGTCAAA+AGG - chr2.1:6435260-6435279 None:intergenic 35.0%
! TGTTTGATGGTTTCTTATGG+AGG + chr2.1:6436642-6436661 MS.gene45530:intron 35.0%
!! ACGAATACTTAGAGCATTGT+CGG + chr2.1:6433871-6433890 MS.gene45530:CDS 35.0%
!! AGATTTCTGAGGTGGTAAAT+AGG + chr2.1:6433766-6433785 MS.gene45530:intron 35.0%
!! GATTTCTGAGGTGGTAAATA+GGG + chr2.1:6433767-6433786 MS.gene45530:intron 35.0%
!! TTACCGGTGTTGTCAAATAT+CGG + chr2.1:6434934-6434953 MS.gene45530:intron 35.0%
!! TTTGAGTTGGACTTATTACC+AGG - chr2.1:6435870-6435889 None:intergenic 35.0%
!!! TTGGCAGAAGTTTTTTGAGT+TGG - chr2.1:6435883-6435902 None:intergenic 35.0%
AAACAGTTAGCAGCATGTGA+CGG - chr2.1:6435401-6435420 None:intergenic 40.0%
AATGGAATTGGCCAACACAT+TGG - chr2.1:6436001-6436020 None:intergenic 40.0%
ACACCAACGCACAATATTGT+TGG + chr2.1:6434375-6434394 MS.gene45530:intron 40.0%
AGAGAAGAAGAAGAGTGAGA+GGG - chr2.1:6434097-6434116 None:intergenic 40.0%
ATATTGGCCGAAATTTGGCA+TGG - chr2.1:6434997-6435016 None:intergenic 40.0%
ATGCCAAATTTCGGCCAATA+TGG + chr2.1:6434996-6435015 MS.gene45530:intron 40.0%
ATGCCAACAATATTGTGCGT+TGG - chr2.1:6434381-6434400 None:intergenic 40.0%
ATGGAATTGGCCAACACATT+GGG - chr2.1:6436000-6436019 None:intergenic 40.0%
ATGTATGAGAGTGGTACAAG+AGG - chr2.1:6435488-6435507 None:intergenic 40.0%
CAAAAGGTCTATGTTAGGAG+GGG + chr2.1:6435274-6435293 MS.gene45530:intron 40.0%
CAATCATGCATCCTAGTGAT+TGG + chr2.1:6436267-6436286 MS.gene45530:intron 40.0%
CCAAGGTTATAAAAACCGGA+CGG - chr2.1:6433979-6433998 None:intergenic 40.0%
CTACTTAACTGAAGGGAACA+GGG + chr2.1:6436466-6436485 MS.gene45530:intron 40.0%
CTGAAAACCAGGGATTAAAC+CGG - chr2.1:6433901-6433920 None:intergenic 40.0%
GAACTAGGAAGAGACTAAAG+TGG - chr2.1:6435451-6435470 None:intergenic 40.0%
GATATTTGACAACACCGGTA+AGG - chr2.1:6434935-6434954 None:intergenic 40.0%
GCAAAAGGTCTATGTTAGGA+GGG + chr2.1:6435273-6435292 MS.gene45530:intron 40.0%
GTCCAAGTGTGTTTGTTTGA+TGG + chr2.1:6436629-6436648 MS.gene45530:intron 40.0%
TAATAGAGACTGCAACAAGC+AGG + chr2.1:6435184-6435203 MS.gene45530:intron 40.0%
TACAAGGTTTCTTCTCTCCA+GGG + chr2.1:6434012-6434031 MS.gene45530:intron 40.0%
TACTTAACTGAAGGGAACAG+GGG + chr2.1:6436467-6436486 MS.gene45530:intron 40.0%
TAGAGAAGAAGAAGAGTGAG+AGG - chr2.1:6434098-6434117 None:intergenic 40.0%
TCTACTTAACTGAAGGGAAC+AGG + chr2.1:6436465-6436484 MS.gene45530:intron 40.0%
TCTGTAGTAACGAACAGAGA+GGG - chr2.1:6434134-6434153 None:intergenic 40.0%
TGCAAAAGGTCTATGTTAGG+AGG + chr2.1:6435272-6435291 MS.gene45530:intron 40.0%
TGTAGGTCATACCAATCACT+AGG - chr2.1:6436281-6436300 None:intergenic 40.0%
TTACAAGGTTTCTTCTCTCC+AGG + chr2.1:6434011-6434030 MS.gene45530:intron 40.0%
! CTTCTGCTGATTATGCTAAC+AGG + chr2.1:6434315-6434334 MS.gene45530:intron 40.0%
! TGCTTGTTGCAGTCTCTATT+AGG - chr2.1:6435185-6435204 None:intergenic 40.0%
! TGTTTTACACTGGGACAAAC+TGG + chr2.1:6435959-6435978 MS.gene45530:intron 40.0%
! TTCAGACTGAGCAGTTTTCA+TGG + chr2.1:6433916-6433935 MS.gene45530:CDS 40.0%
! TTCTATCTGGGTTTTCGATC+AGG - chr2.1:6435137-6435156 None:intergenic 40.0%
!! CTAGTGCTTTTGGGTATTCT+TGG + chr2.1:6437045-6437064 MS.gene45530:CDS 40.0%
!! GGAGGTTTCTTTTGAAGAAC+TGG + chr2.1:6436660-6436679 MS.gene45530:intron 40.0%
!! TTCTAGCTTCTTTGCATTGC+AGG - chr2.1:6436362-6436381 None:intergenic 40.0%
!!! TTAGAGCATTGTCGGTTTTC+CGG + chr2.1:6433879-6433898 MS.gene45530:CDS 40.0%
AAAAATCTTGTACCTGGCGG+TGG - chr2.1:6434420-6434439 None:intergenic 45.0%
AACAGTTAGCAGCATGTGAC+GGG - chr2.1:6435400-6435419 None:intergenic 45.0%
AACCATGTCCCATTGAGTCA+TGG - chr2.1:6436308-6436327 None:intergenic 45.0%
AACTGCTCAGTCTGAAAACC+AGG - chr2.1:6433912-6433931 None:intergenic 45.0%
AATATAACGGCGCCACAAAG+TGG + chr2.1:6435040-6435059 MS.gene45530:intron 45.0%
AATGTGACGCTGCTATGGTA+TGG + chr2.1:6434677-6434696 MS.gene45530:intron 45.0%
ACTGCTCAGTCTGAAAACCA+GGG - chr2.1:6433911-6433930 None:intergenic 45.0%
ACTTTGTGGCGCCGTTATAT+TGG - chr2.1:6435041-6435060 None:intergenic 45.0%
AGGGGAGCTTAATTCCTTAC+CGG + chr2.1:6434918-6434937 MS.gene45530:intron 45.0%
CAAATTTCGGCCAATATGGC+AGG + chr2.1:6435000-6435019 MS.gene45530:intron 45.0%
CGTCTCATCGCAACAATGTT+CGG - chr2.1:6434589-6434608 None:intergenic 45.0%
CTAGGAAGAGACTAAAGTGG+AGG - chr2.1:6435448-6435467 None:intergenic 45.0%
CTGCCATATTGGCCGAAATT+TGG - chr2.1:6435002-6435021 None:intergenic 45.0%
GACTCAATGGGACATGGTTT+AGG + chr2.1:6436309-6436328 MS.gene45530:intron 45.0%
GGTTATAAAAACCGGACGGT+TGG - chr2.1:6433975-6433994 None:intergenic 45.0%
GTCTGTAGTAACGAACAGAG+AGG - chr2.1:6434135-6434154 None:intergenic 45.0%
GTGTTGTCAAATATCGGCCA+TGG + chr2.1:6434940-6434959 MS.gene45530:intron 45.0%
TACAGCCTCCATGACTCAAT+GGG + chr2.1:6436297-6436316 MS.gene45530:intron 45.0%
TGGCCGATATTTGACAACAC+CGG - chr2.1:6434940-6434959 None:intergenic 45.0%
TTCTTCTCTCCAGGGATTGA+GGG + chr2.1:6434020-6434039 MS.gene45530:intron 45.0%
TTGTCAAATATCGGCCATGG+TGG + chr2.1:6434943-6434962 MS.gene45530:intron 45.0%
TTTAGCACCTCCCAATGTGT+TGG + chr2.1:6435987-6436006 MS.gene45530:intron 45.0%
TTTCTTCTCTCCAGGGATTG+AGG + chr2.1:6434019-6434038 MS.gene45530:intron 45.0%
! AGCATCGCGGGTTATAGTTT+GGG - chr2.1:6435829-6435848 None:intergenic 45.0%
! ATCTGAATGGTCACTGGTCT+TGG - chr2.1:6435220-6435239 None:intergenic 45.0%
! CCGTCCGGTTTTTATAACCT+TGG + chr2.1:6433976-6433995 MS.gene45530:intron 45.0%
! GATGATGGCAATGGTGGTAA+TGG + chr2.1:6436991-6437010 MS.gene45530:CDS 45.0%
! TGACGGGTAAGGATCAAGAT+AGG - chr2.1:6435384-6435403 None:intergenic 45.0%
!! ATATCGGCCATGGTGGATAT+TGG + chr2.1:6434950-6434969 MS.gene45530:intron 45.0%
!! GCAGCGTCACATTTTAATCG+AGG - chr2.1:6434670-6434689 None:intergenic 45.0%
!!! GGATATTGGTGGTGGCTTTT+GGG + chr2.1:6434964-6434983 MS.gene45530:intron 45.0%
!!! GGTGGTGGTAATTTTTCAGG+TGG + chr2.1:6437021-6437040 MS.gene45530:CDS 45.0%
!!! TCAGGTGGACTAGTGCTTTT+GGG + chr2.1:6437036-6437055 MS.gene45530:CDS 45.0%
!!! TGGATATTGGTGGTGGCTTT+TGG + chr2.1:6434963-6434982 MS.gene45530:intron 45.0%
!!! TTCAGGTGGACTAGTGCTTT+TGG + chr2.1:6437035-6437054 MS.gene45530:CDS 45.0%
AACGAACAGAGAGGGAGAGA+TGG - chr2.1:6434126-6434145 None:intergenic 50.0%
AAGAAGAGTGAGAGGGTCGA+TGG - chr2.1:6434090-6434109 None:intergenic 50.0%
CATCGCAACAATGTTCGGTC+TGG - chr2.1:6434584-6434603 None:intergenic 50.0%
CATGGTTTAGGCGATAGAGG+TGG + chr2.1:6436321-6436340 MS.gene45530:intron 50.0%
CATGTCCCATTGAGTCATGG+AGG - chr2.1:6436305-6436324 None:intergenic 50.0%
CATTGAGTCATGGAGGCTGT+AGG - chr2.1:6436298-6436317 None:intergenic 50.0%
CCTGGCGGTGGTATTAACAA+AGG - chr2.1:6434408-6434427 None:intergenic 50.0%
CCTTTGTTAATACCACCGCC+AGG + chr2.1:6434405-6434424 MS.gene45530:intron 50.0%
CGCACAATTACGGTTACTCC+TGG + chr2.1:6435849-6435868 MS.gene45530:CDS 50.0%
CTACAGCCTCCATGACTCAA+TGG + chr2.1:6436296-6436315 MS.gene45530:intron 50.0%
CTCACCGCCATGCCAAATTT+CGG + chr2.1:6434987-6435006 MS.gene45530:intron 50.0%
CTCCATGACTCAATGGGACA+TGG + chr2.1:6436303-6436322 MS.gene45530:intron 50.0%
GAATTGGCCAACACATTGGG+AGG - chr2.1:6435997-6436016 None:intergenic 50.0%
GAGCCAAAATCCTGCCAAAC+CGG - chr2.1:6435066-6435085 None:intergenic 50.0%
GGACATGGTTTAGGCGATAG+AGG + chr2.1:6436318-6436337 MS.gene45530:intron 50.0%
GTAAGGAGCAGTGTTGCCAA+TGG - chr2.1:6435103-6435122 None:intergenic 50.0%
TCTTCTCTCCAGGGATTGAG+GGG + chr2.1:6434021-6434040 MS.gene45530:intron 50.0%
TTGGCCGAAATTTGGCATGG+CGG - chr2.1:6434994-6435013 None:intergenic 50.0%
! AGACTTGATGACAATGGGCC+TGG + chr2.1:6436952-6436971 MS.gene45530:CDS 50.0%
! CGGTTTTCCGGTTTAATCCC+TGG + chr2.1:6433891-6433910 MS.gene45530:CDS 50.0%
! GAGCATCGCGGGTTATAGTT+TGG - chr2.1:6435830-6435849 None:intergenic 50.0%
! GTTTTCATGGTCATCCCGAC+TGG + chr2.1:6433929-6433948 MS.gene45530:CDS 50.0%
!! AATGGTGGTAATGGAGGAGG+CGG + chr2.1:6437000-6437019 MS.gene45530:CDS 50.0%
!! GATGGCAATGGTGGTAATGG+AGG + chr2.1:6436994-6437013 MS.gene45530:CDS 50.0%
!! GGCGGTGGTGGTAATTTTTC+AGG + chr2.1:6437018-6437037 MS.gene45530:CDS 50.0%
!! TTAGCAGCATGTGACGGGTA+AGG - chr2.1:6435395-6435414 None:intergenic 50.0%
AACCGGACCAGTATCCAGTC+GGG - chr2.1:6433946-6433965 None:intergenic 55.0%
AAGAGTGAGAGGGTCGATGG+AGG - chr2.1:6434087-6434106 None:intergenic 55.0%
AGAGTGAGAGGGTCGATGGA+GGG - chr2.1:6434086-6434105 None:intergenic 55.0%
AGGAGCAGTGTTGCCAATGG+CGG - chr2.1:6435100-6435119 None:intergenic 55.0%
ATCCCGACTGGATACTGGTC+CGG + chr2.1:6433941-6433960 MS.gene45530:intron 55.0%
CCGCGATGCTCGCACAATTA+CGG + chr2.1:6435839-6435858 MS.gene45530:CDS 55.0%
CTGGATACTGGTCCGGTTCT+CGG + chr2.1:6433948-6433967 MS.gene45530:intron 55.0%
GAACCGGACCAGTATCCAGT+CGG - chr2.1:6433947-6433966 None:intergenic 55.0%
GCCACCACCAATATCCACCA+TGG - chr2.1:6434960-6434979 None:intergenic 55.0%
TGGTCATCCCGACTGGATAC+TGG + chr2.1:6433936-6433955 MS.gene45530:CDS 55.0%
! CCGTAATTGTGCGAGCATCG+CGG - chr2.1:6435842-6435861 None:intergenic 55.0%
! CGTAATTGTGCGAGCATCGC+GGG - chr2.1:6435841-6435860 None:intergenic 55.0%
!! GCCATGGTGGATATTGGTGG+TGG + chr2.1:6434956-6434975 MS.gene45530:intron 55.0%
!! GGCAATGGTGGTAATGGAGG+AGG + chr2.1:6436997-6437016 MS.gene45530:CDS 55.0%
!! TCGGCCATGGTGGATATTGG+TGG + chr2.1:6434953-6434972 MS.gene45530:intron 55.0%
!!! AGGATTTTGGCTCTCCGCCA+TGG + chr2.1:6435073-6435092 MS.gene45530:intron 55.0%
!!! TGGCCGGTTTGGCAGGATTT+TGG + chr2.1:6435060-6435079 MS.gene45530:intron 55.0%
CCATTGCCATCATCGTCGCG+AGG - chr2.1:6436985-6437004 None:intergenic 60.0%
GTGTTGCCAATGGCGGTCCA+TGG - chr2.1:6435093-6435112 None:intergenic 60.0%
TAACGGCGCCACAAAGTGGC+CGG + chr2.1:6435044-6435063 MS.gene45530:intron 60.0%
TTGCCAATGGCGGTCCATGG+CGG - chr2.1:6435090-6435109 None:intergenic 60.0%
TTGCCACCTCGCGACGATGA+TGG + chr2.1:6436976-6436995 MS.gene45530:CDS 60.0%
TTGCCATCATCGTCGCGAGG+TGG - chr2.1:6436982-6437001 None:intergenic 60.0%
! CCTCGCGACGATGATGGCAA+TGG + chr2.1:6436982-6437001 MS.gene45530:CDS 60.0%
! CGCGACGATGATGGCAATGG+TGG + chr2.1:6436985-6437004 MS.gene45530:CDS 60.0%
! CTGCCAAACCGGCCACTTTG+TGG - chr2.1:6435055-6435074 None:intergenic 60.0%
!! CACAAAGTGGCCGGTTTGGC+AGG + chr2.1:6435053-6435072 MS.gene45530:intron 60.0%
CGGACGGTTGGACCGAGAAC+CGG - chr2.1:6433963-6433982 None:intergenic 65.0%
CGGTTCTCGGTCCAACCGTC+CGG + chr2.1:6433961-6433980 MS.gene45530:intron 65.0%
GAGGGAGTCGCGAGCAGTGA+AGG - chr2.1:6434068-6434087 None:intergenic 65.0%
GCGCCACAAAGTGGCCGGTT+TGG + chr2.1:6435049-6435068 MS.gene45530:intron 65.0%
GCTTGCGTCCCCTCAATCCC+TGG - chr2.1:6434032-6434051 None:intergenic 65.0%
GGAGTCGCGAGCAGTGAAGG+TGG - chr2.1:6434065-6434084 None:intergenic 65.0%
GTCGCGAGCAGTGAAGGTGG+CGG - chr2.1:6434062-6434081 None:intergenic 65.0%
TCGTCGCGAGGTGGCAATCC+AGG - chr2.1:6436973-6436992 None:intergenic 65.0%
TCTCCGCCATGGACCGCCAT+TGG + chr2.1:6435084-6435103 MS.gene45530:intron 65.0%
!! GGTAATGGAGGAGGCGGTGG+TGG + chr2.1:6437006-6437025 MS.gene45530:CDS 65.0%
!! GGTGGTAATGGAGGAGGCGG+TGG + chr2.1:6437003-6437022 MS.gene45530:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 6433717 6437143 6433717 ID=MS.gene45530
chr2.1 mRNA 6433717 6437143 6433717 ID=MS.gene45530.t1;Parent=MS.gene45530
chr2.1 exon 6433717 6433721 6433717 ID=MS.gene45530.t1.exon1;Parent=MS.gene45530.t1
chr2.1 CDS 6433717 6433721 6433717 ID=cds.MS.gene45530.t1;Parent=MS.gene45530.t1
chr2.1 exon 6433848 6433957 6433848 ID=MS.gene45530.t1.exon2;Parent=MS.gene45530.t1
chr2.1 CDS 6433848 6433957 6433848 ID=cds.MS.gene45530.t1;Parent=MS.gene45530.t1
chr2.1 exon 6434033 6434138 6434033 ID=MS.gene45530.t1.exon3;Parent=MS.gene45530.t1
chr2.1 CDS 6434033 6434138 6434033 ID=cds.MS.gene45530.t1;Parent=MS.gene45530.t1
chr2.1 exon 6434549 6434693 6434549 ID=MS.gene45530.t1.exon4;Parent=MS.gene45530.t1
chr2.1 CDS 6434549 6434693 6434549 ID=cds.MS.gene45530.t1;Parent=MS.gene45530.t1
chr2.1 exon 6435798 6435870 6435798 ID=MS.gene45530.t1.exon5;Parent=MS.gene45530.t1
chr2.1 CDS 6435798 6435870 6435798 ID=cds.MS.gene45530.t1;Parent=MS.gene45530.t1
chr2.1 exon 6436941 6437143 6436941 ID=MS.gene45530.t1.exon6;Parent=MS.gene45530.t1
chr2.1 CDS 6436941 6437143 6436941 ID=cds.MS.gene45530.t1;Parent=MS.gene45530.t1
Gene Sequence

>MS.gene45530

ATGGGGTAAAATTAATAAAATACTAAATTATACTATTAATTATTATGTTAGATTTCTGAGGTGGTAAATAGGGATACAATAATTTTCCTTAAAGAAAAAAGGATACAATATTTTCTTCTTTTTGTTTTCAGTACTTTGTTCAAATTAAAATCAGACGAATACTTAGAGCATTGTCGGTTTTCCGGTTTAATCCCTGGTTTTCAGACTGAGCAGTTTTCATGGTCATCCCGACTGGATACTGGTCCGGTTCTCGGTCCAACCGTCCGGTTTTTATAACCTTGGTTAATATGAAAATTACAAGGTTTCTTCTCTCCAGGGATTGAGGGGACGCAAGCTGACTCTCCGCCACCTTCACTGCTCGCGACTCCCTCCATCGACCCTCTCACTCTTCTTCTTCTCTACGTATCCATCTCTCCCTCTCTGTTCGTTACTACAGACCCATAAACCCTAATTGTACGTTGTTTTTTCAATCTCCTCGATATATTTTCCTTTTTCCCTTCAATTTTTTAATCTTATTGTGTTGGTTCTTAGCTGTTATTGTTGTTGATGATGAATTTTGAATTTGAGTTTTTGACTAATAGTTTGTTTTTGATGCGTTCTTCTGCTGATTATGCTAACAGGTAGCATTGTTTAACATTTGGTCATAATAAAGATGTTGACACCAACGCACAATATTGTTGGCATTTCACCTTTGTTAATACCACCGCCAGGTACAAGATTTTTAGTTGAGATAGTTTTAGCAACTTAGGTTTTTGAAATGTGAATTTAGTTTATATAAATCATTCATGGATATTTTTATTTTTTTTGTTGGTATTTTCTCGTTTGCAATCAGTGCAAGTCGAACTCAGACACGTCTTAAGCATTCCAGACCGAACATTGTTGCGATGAGACGCATACCATATAACATAAAAAAAGGGCAGCTTCAGACTTCAGCTTTTCAAACTATATCACCTCGATTAAAATGTGACGCTGCTATGGTATGGTTTTTTATTTTGGAATTTGTTGATTGTTTGCTTTCTTTATGCTATGGTATGGTTATTTTTATTTCTGTTGATTGTTTGCTTTCTTTATGCTAAATGATATTCTTGTTGGAAAGTATTAGCAGCGTTTTGTGTTATTGCAATGTCAAATCAGTCCTTTTTGATAAGTTGGTAAGGTTCCAAAATAAAACTGTTAATTGATGAATAATTTTTATCTTGCAAGGGGAGCTTAATTCCTTACCGGTGTTGTCAAATATCGGCCATGGTGGATATTGGTGGTGGCTTTTGGGCTCACCGCCATGCCAAATTTCGGCCAATATGGCAGGATTTTATATCATAATCCAATATAACGGCGCCACAAAGTGGCCGGTTTGGCAGGATTTTGGCTCTCCGCCATGGACCGCCATTGGCAACACTGCTCCTTACTATAGAAGAAACCTGATCGAAAACCCAGATAGAATAGGAAAATAGATTATAAAATGATACCTAATAGAGACTGCAACAAGCAGGCAGACTGCAACCAAGACCAGTGACCATTCAGATATGTTTCTGATAGGAAACCTTTTGACCTTTTGTGCAAAAGGTCTATGTTAGGAGGGGTAATGTATTATGTAGAGATAGAAATGTAACTAACAGTTCCATTCTTGTTATTTTCCTTTAACTTCTGTTGAATCCTATCACTTTCCTATCTTGATCCTTACCCGTCACATGCTGCTAACTGTTTCCACTGTTTCTCTTCTATCTGAATCCTCCACTTTAGTCTCTTCCTAGTTCCAATTTCTTCATTGCCTCTTGTACCACTCTCATACATATGAAACTTTTTAAGTAGAGAAGTTTAATGTCTGTTTGTTGACTGTTGATTGATTCGCTGCATGTTCCATTGATTTTTCTGTAGGCATTTTTTATAGGATGGAATATGGAAGTAAATGTTGTTTGATCTTTTGTTCTTACTTTCAATCCGAATCATGGAAATTTTGGATTTTATTGGTGAATCATTGACTAATAGAAAAACTTATCCAAAATTGTTTGTTGGTCTACGATATTCTGATGTCTTGTATGGTGCTCATATTCGGATGTCTTGTATGGCACCCCTTTCTACAGAATGTTACATGTGCTGCTGCTGCTGCAACCCAAACTATAACCCGCGATGCTCGCACAATTACGGTTACTCCTGGTAATAAGTCCAACTCAAAAAACTTCTGCCAAAAAATGATATGATGTTGGAGAAAGTATATAATGTTAGATTGCTAAGTTGTATTGTTTGTTTTACACTGGGACAAACTGGAACTATTTAGCACCTCCCAATGTGTTGGCCAATTCCATTTTCTATACTAGTCATCATGTGCATATTAGTAAATAGTTTATGTATATAAATTTCATTCTTCTTTCTTCTGCCATACATCAGTGTCTTTATTGAAATAACTAAATCAATATCAAAGCGTAGGTTTTTTATGTGAGAAACAAATATGAATATTTCATAATTAAGGTATGCACACTCAAATGTTTGTTTCTTTCAAATAGAAGTCATTTGAAGTGCATAAATAATGGCTTTCTTGGATCTTTTCCTTTACAATCATGCATCCTAGTGATTGGTATGACCTACAGCCTCCATGACTCAATGGGACATGGTTTAGGCGATAGAGGTGGTTTTCTTGCTTAATACCTGCAATGCAAAGAAGCTAGAAAAGGAACTCAAATGAGTAGCAACAACAGTAAATTTATAAAGGATTTTGATATTAGAAGGTTAAAATAAATAGTTTCAAATCTGTCTACTTAACTGAAGGGAACAGGGGATTTTTATTTTTTGGTGCTCCTACAATCTTATTAGTTTTTTTTTTGACAACACAATCTTATTAGTTTTTCCCTTCTGTAGATAGTGTTTTGTAGTCTGTTTCCTAGAATGGTTTCGTTTCAGTTTTGTTGTCTTTATTTGTCCAAGTGTGTTTGTTTGATGGTTTCTTATGGAGGTTTCTTTTGAAGAACTGGAAACATTTACTACAAATTGCACGACTTGACATTTTGTAAATAAATAATAATATATAGCATTTGTCATAATATGTGGACGGATACTATTGAAATCCACAACTCAACTCTATTCATTTACCTTTTCTACAAAATTAGCAATTATGTACATGTAAATGTTGTTTCACCCTTAATTGATTTTTTATTCTGTGTTTCGAATAATTATACGTTCCTTGTCTATGTATACAAATATAATTTGCTATCATTTTCCTGAAATGACAGACAAAAAGATTAGACTTGATGACAATGGGCCTGGATTGCCACCTCGCGACGATGATGGCAATGGTGGTAATGGAGGAGGCGGTGGTGGTAATTTTTCAGGTGGACTAGTGCTTTTGGGTATTCTTGGTGTCCTAGACATTCTTAAAGACATTGAGAGTGAAATTCAACGCAAAGTCAAAGATAGCAGATTTGATCAAGCTTGA
Protein sequence

>MS.gene45530.t1

MGTLFKLKSDEYLEHCRFSGLIPGFQTEQFSWSSRLDTGIEGTQADSPPPSLLATPSIDPLTLLLLYVSISPSLASRTQTRLKHSRPNIVAMRRIPYNIKKGQLQTSAFQTISPRLKCDAAMNVTCAAAAATQTITRDARTITVTPDKKIRLDDNGPGLPPRDDDGNGGNGGGGGGNFSGGLVLLGILGVLDILKDIESEIQRKVKDSRFDQA