AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45579


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45579.t1 MTR_3g037560 96.091 486 19 0 13 498 55 540 0.0 976
MS.gene45579.t1 MTR_4g108780 80.777 489 91 1 8 493 85 573 0.0 830
MS.gene45579.t1 MTR_8g099865 50.638 470 221 4 31 494 120 584 3.54e-165 480
MS.gene45579.t1 MTR_1g090430 49.378 482 230 4 20 493 11 486 8.14e-165 475
MS.gene45579.t1 MTR_2g020730 44.515 474 243 8 30 492 111 575 2.89e-135 403
MS.gene45579.t1 MTR_4g007220 43.949 471 247 7 30 493 137 597 3.45e-132 396
MS.gene45579.t1 MTR_4g007360 32.456 228 125 6 17 243 115 314 5.03e-25 105
MS.gene45579.t1 MTR_7g005890 37.037 135 61 3 254 386 4 116 3.37e-16 75.1
MS.gene45579.t1 MTR_4g007380 41.818 110 48 3 343 452 9 102 2.88e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45579.t1 AT2G03220 65.849 489 157 3 13 491 70 558 0.0 669
MS.gene45579.t1 AT2G03210 60.243 493 179 6 17 493 78 569 0.0 618
MS.gene45579.t1 AT2G03210 60.825 485 176 6 19 493 92 572 0.0 617
MS.gene45579.t1 AT2G03210 60.825 485 176 6 19 493 58 538 0.0 616
MS.gene45579.t1 AT1G14100 56.008 491 200 6 16 494 30 516 0.0 549
MS.gene45579.t1 AT1G14080 52.941 493 218 5 13 493 46 536 0.0 534
MS.gene45579.t1 AT2G15350 51.919 495 224 9 11 493 21 513 3.37e-177 508
MS.gene45579.t1 AT2G15390 51.760 483 221 6 22 493 21 502 1.21e-176 506
MS.gene45579.t1 AT2G15390 51.767 481 220 6 23 492 54 533 3.92e-176 506
MS.gene45579.t1 AT2G15370 50.947 475 224 6 27 492 57 531 5.17e-169 488
MS.gene45579.t1 AT1G14110 51.875 480 179 7 20 492 35 469 2.56e-168 484
MS.gene45579.t1 AT1G74420 52.106 451 193 9 23 464 88 524 4.46e-157 457
MS.gene45579.t1 AT1G74420 52.477 444 188 9 30 464 91 520 8.64e-157 456
MS.gene45579.t1 AT1G74420 52.106 451 193 9 23 464 126 562 2.04e-156 457
MS.gene45579.t1 AT1G74420 52.477 444 188 9 30 464 129 558 4.81e-156 456

Find 0 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCATATTATTTGGTTATT+AGG - 23426:69429-69448 MS.gene45579:CDS 15.0%
!! AACCAATAAATGCATAAAAT+AGG + 23426:69356-69375 None:intergenic 20.0%
!! TGATAGAGTTTCATATTATT+TGG - 23426:69421-69440 MS.gene45579:CDS 20.0%
!!! ATCTTTTACTGTTTTATTGT+AGG + 23426:69658-69677 None:intergenic 20.0%
! AAAAAGTAAGAGACATGTAT+TGG - 23426:70471-70490 MS.gene45579:CDS 25.0%
! AAAAGTAAGAGACATGTATT+GGG - 23426:70472-70491 MS.gene45579:CDS 25.0%
! AAATACACAATCAAAAAGCT+TGG - 23426:70570-70589 MS.gene45579:CDS 25.0%
! AATACACAATCAAAAAGCTT+GGG - 23426:70571-70590 MS.gene45579:CDS 25.0%
!! AAAAAATTTGAAGTCGAAAG+CGG - 23426:70415-70434 MS.gene45579:CDS 25.0%
!! ATCTTTTTGGATACTTGAAT+AGG - 23426:69273-69292 MS.gene45579:CDS 25.0%
!! GTCCTATTTTATGCATTTAT+TGG - 23426:69351-69370 MS.gene45579:CDS 25.0%
!! TTTCTCATTTGCATAACTTT+TGG - 23426:69390-69409 MS.gene45579:CDS 25.0%
!!! TACTGTTTTATTGTAGGATT+CGG + 23426:69652-69671 None:intergenic 25.0%
!!! TGCATTTATTGGTTTTCTAT+TGG - 23426:69362-69381 MS.gene45579:CDS 25.0%
AAAAACTAACATCCCAATTC+AGG + 23426:69301-69320 None:intergenic 30.0%
AAAACTAACATCCCAATTCA+GGG + 23426:69300-69319 None:intergenic 30.0%
ACAGTAAAAGATCTCAATTC+TGG - 23426:69666-69685 MS.gene45579:CDS 30.0%
ATCCAAAAAGATAGTCTACA+TGG + 23426:69265-69284 None:intergenic 30.0%
ATCTTCGTAATCTTGAAATG+AGG + 23426:69596-69615 None:intergenic 30.0%
ATTCAGATAAAGGACTATCA+GGG - 23426:69556-69575 MS.gene45579:CDS 30.0%
GTAGTTTGGATTTCATTTAG+CGG - 23426:69720-69739 MS.gene45579:CDS 30.0%
TAAGAAATTGACTGTTGAGA+GGG + 23426:69893-69912 None:intergenic 30.0%
TGATCAAACACATGTTGAAA+TGG + 23426:70323-70342 None:intergenic 30.0%
TTCCCAAAAGATAAACTACT+TGG - 23426:69483-69502 MS.gene45579:CDS 30.0%
TTCGAAATAACCAGAGTTTA+AGG + 23426:70453-70472 None:intergenic 30.0%
! CACCAAGTAGTTTATCTTTT+GGG + 23426:69488-69507 None:intergenic 30.0%
! GACAAAGTTTTCCATTTCTT+AGG - 23426:70173-70192 MS.gene45579:CDS 30.0%
! GACAAGAAGCATGATAATAT+TGG - 23426:70386-70405 MS.gene45579:CDS 30.0%
! TCTGTTTTCATCATTAACCA+AGG + 23426:70080-70099 None:intergenic 30.0%
! TTATTGTAGGATTCGGTATA+AGG + 23426:69645-69664 None:intergenic 30.0%
!! AATCTTAGCTTGTACTTTGA+AGG - 23426:70343-70362 MS.gene45579:CDS 30.0%
!! TTAGCCTTACAATCATAGAA+AGG + 23426:70779-70798 None:intergenic 30.0%
AAACACATGTTGAAATGGAC+CGG + 23426:70318-70337 None:intergenic 35.0%
AAACTACGTACTTACAGTCT+GGG + 23426:69707-69726 None:intergenic 35.0%
AAATGAAGGTAGACAGAAGA+AGG + 23426:69987-70006 None:intergenic 35.0%
AATTTCTGCAGAAAGTTCCT+TGG - 23426:70060-70079 MS.gene45579:CDS 35.0%
ACAAATACCTGCCTAAGAAA+TGG + 23426:70187-70206 None:intergenic 35.0%
ACAGCATCAAGAATAACGAT+GGG + 23426:70115-70134 None:intergenic 35.0%
ACATTGTGAGGATATGAGTT+GGG - 23426:70838-70857 MS.gene45579:intron 35.0%
ATACAGAAATGCAGAAGCTA+AGG + 23426:69766-69785 None:intergenic 35.0%
ATTGAGTTTAACCGATGTGT+TGG - 23426:70607-70626 MS.gene45579:CDS 35.0%
CAGTAATCTCTTTCCAAACA+AGG - 23426:70151-70170 MS.gene45579:CDS 35.0%
CATAATCATGCACCAAATGA+AGG + 23426:70001-70020 None:intergenic 35.0%
CATTCAGATAAAGGACTATC+AGG - 23426:69555-69574 MS.gene45579:CDS 35.0%
CCAAAAGATAAACTACTTGG+TGG - 23426:69486-69505 MS.gene45579:CDS 35.0%
CGTAATCTTGAAATGAGGTA+AGG + 23426:69591-69610 None:intergenic 35.0%
CTAAGAAATTGACTGTTGAG+AGG + 23426:69894-69913 None:intergenic 35.0%
CTAATGACATCCTTAAACTC+TGG - 23426:70440-70459 MS.gene45579:CDS 35.0%
GGATTTCATTTAGCGGTTTA+GGG - 23426:69727-69746 MS.gene45579:CDS 35.0%
GTAAACACCAATAACTTCTC+CGG + 23426:70513-70532 None:intergenic 35.0%
GTCCTTTATCTGAATGATAC+CGG + 23426:69551-69570 None:intergenic 35.0%
TACTTGTTTACATGCTCTGT+TGG - 23426:69327-69346 MS.gene45579:CDS 35.0%
TATGATTGTAAGGCTAAGAG+AGG - 23426:70782-70801 MS.gene45579:intron 35.0%
TCACCCTACAAACAAAGTTT+GGG - 23426:70208-70227 MS.gene45579:CDS 35.0%
TCAGGTTTGTATAGTATCCA+AGG + 23426:70689-70708 None:intergenic 35.0%
TCCTCCTTTCTATGATTGTA+AGG - 23426:70772-70791 MS.gene45579:intron 35.0%
TGGAAAACTTTGTCCTTGTT+TGG + 23426:70167-70186 None:intergenic 35.0%
TGGATTTCATTTAGCGGTTT+AGG - 23426:69726-69745 MS.gene45579:CDS 35.0%
TTCACCCTACAAACAAAGTT+TGG - 23426:70207-70226 MS.gene45579:CDS 35.0%
TTCTTCTGTCTACCTTCATT+TGG - 23426:69986-70005 MS.gene45579:CDS 35.0%
! AAGTTTTCCATTTCTTAGGC+AGG - 23426:70177-70196 MS.gene45579:CDS 35.0%
! AATCCCCAAACTTTGTTTGT+AGG + 23426:70215-70234 None:intergenic 35.0%
! ATCCCCAAACTTTGTTTGTA+GGG + 23426:70214-70233 None:intergenic 35.0%
! CCACCAAGTAGTTTATCTTT+TGG + 23426:69489-69508 None:intergenic 35.0%
! GACCATGTAGACTATCTTTT+TGG - 23426:69260-69279 MS.gene45579:CDS 35.0%
!!! TATAAGGTCCGCATTTTTTG+TGG + 23426:69629-69648 None:intergenic 35.0%
AACACATGTTGAAATGGACC+GGG + 23426:70317-70336 None:intergenic 40.0%
AACTACGTACTTACAGTCTG+GGG + 23426:69706-69725 None:intergenic 40.0%
ACTAGCTCATGGTCTACTTT+CGG - 23426:70632-70651 MS.gene45579:CDS 40.0%
ACTTGAATAGGTCCCTGAAT+TGG - 23426:69285-69304 MS.gene45579:CDS 40.0%
AGCCGGTATCATTCAGATAA+AGG - 23426:69546-69565 MS.gene45579:CDS 40.0%
CAAACTACGTACTTACAGTC+TGG + 23426:69708-69727 None:intergenic 40.0%
CACCCTACAAACAAAGTTTG+GGG - 23426:70209-70228 MS.gene45579:CDS 40.0%
CAGAAATCTGATCACTGTCA+TGG - 23426:69921-69940 MS.gene45579:CDS 40.0%
CAGACTGTAAGTACGTAGTT+TGG - 23426:69706-69725 MS.gene45579:CDS 40.0%
CATTGTGAGGATATGAGTTG+GGG - 23426:70839-70858 MS.gene45579:intron 40.0%
CCAAAAGAACACGATTTGTG+AGG + 23426:69794-69813 None:intergenic 40.0%
CTTGAAATGAGGTAAGGAGA+TGG + 23426:69585-69604 None:intergenic 40.0%
CTTGAATAGGTCCCTGAATT+GGG - 23426:69286-69305 MS.gene45579:CDS 40.0%
GAAGCTCTCCACAAAAAATG+CGG - 23426:69618-69637 MS.gene45579:CDS 40.0%
GACAGCATCAAGAATAACGA+TGG + 23426:70116-70135 None:intergenic 40.0%
GACATTGTGAGGATATGAGT+TGG - 23426:70837-70856 MS.gene45579:intron 40.0%
GCCTTACAATCATAGAAAGG+AGG + 23426:70776-70795 None:intergenic 40.0%
TCATGCTTCTTGTCAACATC+AGG + 23426:70380-70399 None:intergenic 40.0%
TCCTCACAATGTCTTACATG+AGG + 23426:70830-70849 None:intergenic 40.0%
TCCTCATGTAAGACATTGTG+AGG - 23426:70826-70845 MS.gene45579:intron 40.0%
! AAAGGAGGAGCATGAAAACA+CGG + 23426:70761-70780 None:intergenic 40.0%
! CCTCACAAATCGTGTTCTTT+TGG - 23426:69791-69810 MS.gene45579:CDS 40.0%
! CTTGATGCTGTCTTTTGATC+AGG - 23426:70124-70143 MS.gene45579:CDS 40.0%
! GGTGCATGATTATGATGATG+AGG - 23426:70007-70026 MS.gene45579:CDS 40.0%
!! CAGTTTGTTGATATCCCTCA+TGG + 23426:70544-70563 None:intergenic 40.0%
!! GTAGACAGAAGAAGGTACTT+TGG + 23426:69979-69998 None:intergenic 40.0%
!! TGGGAACTCTTTAGAATCAG+AGG + 23426:69469-69488 None:intergenic 40.0%
AAAGAGTCGGCATCCAGATT+AGG - 23426:70276-70295 MS.gene45579:CDS 45.0%
AAGAGTCGGCATCCAGATTA+GGG - 23426:70277-70296 MS.gene45579:CDS 45.0%
ACTGTGACCGGAGAAGTTAT+TGG - 23426:70503-70522 MS.gene45579:CDS 45.0%
AGTCTGGGGACTCACTTAAA+TGG + 23426:69692-69711 None:intergenic 45.0%
ATAACTTCTCCGGTCACAGT+TGG + 23426:70503-70522 None:intergenic 45.0%
ATGATAGATCCTGTCTCAGC+CGG - 23426:69529-69548 MS.gene45579:CDS 45.0%
ATTGACATTGCACGTCGACA+AGG + 23426:70734-70753 None:intergenic 45.0%
CATTTAGCGGTTTAGGGAAC+AGG - 23426:69733-69752 MS.gene45579:CDS 45.0%
GTTTACCAACCAAGCCATGA+GGG - 23426:70527-70546 MS.gene45579:CDS 45.0%
TCGTGTCGAAAACCCTAATC+TGG + 23426:70292-70311 None:intergenic 45.0%
TGACTGTTGAGAGGGAAATC+AGG + 23426:69885-69904 None:intergenic 45.0%
TGAGCTAGTCACCAACACAT+CGG + 23426:70621-70640 None:intergenic 45.0%
TGGGAATATCCAACTGTGAC+CGG - 23426:70491-70510 MS.gene45579:CDS 45.0%
TGTTGAGAGGGAAATCAGGA+GGG + 23426:69881-69900 None:intergenic 45.0%
TGTTGATATCCCTCATGGCT+TGG + 23426:70539-70558 None:intergenic 45.0%
TGTTTACCAACCAAGCCATG+AGG - 23426:70526-70545 MS.gene45579:CDS 45.0%
TTGTCGACGTGCAATGTCAA+TGG - 23426:70733-70752 MS.gene45579:intron 45.0%
! AACCAGGAGACATCTGGAAT+CGG + 23426:69855-69874 None:intergenic 45.0%
! GCCAACGTTGATGAAAGAGT+CGG - 23426:70263-70282 MS.gene45579:CDS 45.0%
! GGATATGAGTTGGGGTCTTA+AGG - 23426:70847-70866 MS.gene45579:CDS 45.0%
!! ATGTGTTGGTGACTAGCTCA+TGG - 23426:70621-70640 MS.gene45579:CDS 45.0%
CTGTTGAGAGGGAAATCAGG+AGG + 23426:69882-69901 None:intergenic 50.0%
GAAATCAGGAGGGAGAAACC+AGG + 23426:69871-69890 None:intergenic 50.0%
TCAGGAGCAGTTTCGTTCTC+AGG + 23426:70707-70726 None:intergenic 50.0%
! AACCGATTCCAGATGTCTCC+TGG - 23426:69850-69869 MS.gene45579:CDS 50.0%
! GCCGACTCTTTCATCAACGT+TGG + 23426:70267-70286 None:intergenic 50.0%
! TGAATGATACCGGCTGAGAC+AGG + 23426:69541-69560 None:intergenic 50.0%
!! AGATCGACCATATCGACTCC+AGG + 23426:69822-69841 None:intergenic 50.0%
!! CGTGTTCTTTTGGTTGACCC+TGG - 23426:69801-69820 MS.gene45579:CDS 50.0%
!! CTTGGTGGACTTCTAGCATC+TGG - 23426:69501-69520 MS.gene45579:CDS 50.0%
!! GATATCCCTCATGGCTTGGT+TGG + 23426:70535-70554 None:intergenic 50.0%
!! GATCGACCATATCGACTCCA+GGG + 23426:69821-69840 None:intergenic 50.0%
ACTTTCGGCTACGTCGCACA+GGG - 23426:70647-70666 MS.gene45579:CDS 55.0%
GACATTGCACGTCGACAAGG+AGG + 23426:70731-70750 None:intergenic 55.0%
GGGAGAAACCAGGAGACATC+TGG + 23426:69861-69880 None:intergenic 55.0%
GGTTGACCCTGGAGTCGATA+TGG - 23426:69812-69831 MS.gene45579:CDS 55.0%
TACTTTCGGCTACGTCGCAC+AGG - 23426:70646-70665 MS.gene45579:CDS 55.0%
! AGGGTTTTCGACACGAGACC+CGG - 23426:70296-70315 MS.gene45579:CDS 55.0%
! GGCTCGGAGGTTTGAAACCT+TGG - 23426:70669-70688 MS.gene45579:CDS 55.0%
!! GCTAAGAGAGGCACTGACAC+AGG - 23426:70794-70813 MS.gene45579:intron 55.0%
CTTTCGGCTACGTCGCACAG+GGG - 23426:70648-70667 MS.gene45579:CDS 60.0%
GCACGTCGACAAGGAGGATC+AGG + 23426:70725-70744 None:intergenic 60.0%
GGCTACGTCGCACAGGGGCT+CGG - 23426:70653-70672 MS.gene45579:CDS 70.0%
TACGTCGCACAGGGGCTCGG+AGG - 23426:70656-70675 MS.gene45579:CDS 70.0%


Chromosome Type Strat End Strand Name
23426 gene 69254 70898 69254 ID=MS.gene45579
23426 mRNA 69254 70898 69254 ID=MS.gene45579.t1;Parent=MS.gene45579
23426 exon 70842 70898 70842 ID=MS.gene45579.t1.exon1;Parent=MS.gene45579.t1
23426 CDS 70842 70898 70842 ID=cds.MS.gene45579.t1;Parent=MS.gene45579.t1
23426 exon 69254 70693 69254 ID=MS.gene45579.t1.exon2;Parent=MS.gene45579.t1
23426 CDS 69254 70693 69254 ID=cds.MS.gene45579.t1;Parent=MS.gene45579.t1
Gene Sequence

>MS.gene45579

ATGTATGACCATGTAGACTATCTTTTTGGATACTTGAATAGGTCCCTGAATTGGGATGTTAGTTTTTGCTTACTACTTGTTTACATGCTCTGTTGGTGTCCTATTTTATGCATTTATTGGTTTTCTATTGGTTTTGTTTCTCATTTGCATAACTTTTGGTCTTATAATGATAGAGTTTCATATTATTTGGTTATTAGGTTTGAAGAGTGATGCCTCTGATTCTAAAGAGTTCCCAAAAGATAAACTACTTGGTGGACTTCTAGCATCTGGATTTGATGATAGATCCTGTCTCAGCCGGTATCATTCAGATAAAGGACTATCAGGGAATCCATCTCCTTACCTCATTTCAAGATTACGAAGATACGAAGCTCTCCACAAAAAATGCGGACCTTATACCGAATCCTACAATAAAACAGTAAAAGATCTCAATTCTGGCCATTTAAGTGAGTCCCCAGACTGTAAGTACGTAGTTTGGATTTCATTTAGCGGTTTAGGGAACAGGATTTTGACCTTAGCTTCTGCATTTCTGTATGCTCTCCTCACAAATCGTGTTCTTTTGGTTGACCCTGGAGTCGATATGGTCGATCTCTTTTGTGAACCGATTCCAGATGTCTCCTGGTTTCTCCCTCCTGATTTCCCTCTCAACAGTCAATTTCTTAGTTTGAATCAGAAATCTGATCACTGTCATGGAAAAATGCTAAAAAATAAATCAGTCACAAATTCCAAAGTACCTTCTTCTGTCTACCTTCATTTGGTGCATGATTATGATGATGAGGATAAGCTTTTTTTCTGTGATGAAGAACAAAAATTTCTGCAGAAAGTTCCTTGGTTAATGATGAAAACAGATAATTACTTTATCCCATCGTTATTCTTGATGCTGTCTTTTGATCAGGAGCTCAGTAATCTCTTTCCAAACAAGGACAAAGTTTTCCATTTCTTAGGCAGGTATTTGTTTCACCCTACAAACAAAGTTTGGGGATTTGTTTCTAGATACTATCAAGCTTATTTAGCCAACGTTGATGAAAGAGTCGGCATCCAGATTAGGGTTTTCGACACGAGACCCGGTCCATTTCAACATGTGTTTGATCAAATCTTAGCTTGTACTTTGAAGGAGAATCTCTTGCCTGATGTTGACAAGAAGCATGATAATATTGGTTCATTAAAAAATTTGAAGTCGAAAGCGGTACTAATGACATCCTTAAACTCTGGTTATTTCGAAAAAGTAAGAGACATGTATTGGGAATATCCAACTGTGACCGGAGAAGTTATTGGTGTTTACCAACCAAGCCATGAGGGATATCAACAAACTGAGAAGCAAATACACAATCAAAAAGCTTGGGCAGAAATGTATCTATTGAGTTTAACCGATGTGTTGGTGACTAGCTCATGGTCTACTTTCGGCTACGTCGCACAGGGGCTCGGAGGTTTGAAACCTTGGATACTATACAAACCTGAGAACGAAACTGCTCCTGATCCTCCTTGTCGACGTGCAATGTCAATGGAACCGTGTTTTCATGCTCCTCCTTTCTATGATTGTAAGGCTAAGAGAGGCACTGACACAGGTGCACTTGTTCCTCATGTAAGACATTGTGAGGATATGAGTTGGGGTCTTAAGGTTGTAGACAATAATAGTGCTAGTTATTGA

Protein sequence

>MS.gene45579.t1

MYDHVDYLFGYLNRSLNWDSDASDSKEFPKDKLLGGLLASGFDDRSCLSRYHSDKGLSGNPSPYLISRLRRYEALHKKCGPYTESYNKTVKDLNSGHLSESPDCKYVVWISFSGLGNRILTLASAFLYALLTNRVLLVDPGVDMVDLFCEPIPDVSWFLPPDFPLNSQFLSLNQKSDHCHGKMLKNKSVTNSKVPSSVYLHLVHDYDDEDKLFFCDEEQKFLQKVPWLMMKTDNYFIPSLFLMLSFDQELSNLFPNKDKVFHFLGRYLFHPTNKVWGFVSRYYQAYLANVDERVGIQIRVFDTRPGPFQHVFDQILACTLKENLLPDVDKKHDNIGSLKNLKSKAVLMTSLNSGYFEKVRDMYWEYPTVTGEVIGVYQPSHEGYQQTEKQIHNQKAWAEMYLLSLTDVLVTSSWSTFGYVAQGLGGLKPWILYKPENETAPDPPCRRAMSMEPCFHAPPFYDCKAKRGTDTGALVPHVRHCEDMSWGLKVVDNNSASY