AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45667

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45667.t1 MTR_4g070140 97.895 190 3 1 1 189 1 190 1.08e-109 311
MS.gene45667.t1 MTR_4g070190 88.372 86 10 0 1 86 1 86 4.36e-52 164
MS.gene45667.t1 MTR_4g070080 83.168 101 17 0 4 104 2 102 1.22e-47 152
MS.gene45667.t1 MTR_4g070080 83.673 98 16 0 4 101 2 99 1.40e-46 150
MS.gene45667.t1 MTR_3g025880 47.059 85 45 0 3 87 39 123 8.33e-23 94.7
MS.gene45667.t1 MTR_3g075500 51.852 81 39 0 8 88 206 286 9.18e-23 92.8
MS.gene45667.t1 MTR_3g075500 39.506 81 49 0 9 89 110 190 3.15e-11 61.2
MS.gene45667.t1 MTR_4g109540 47.500 80 42 0 9 88 43 122 2.96e-22 90.9
MS.gene45667.t1 MTR_7g013730 44.578 83 46 0 9 91 41 123 8.51e-22 89.4
MS.gene45667.t1 MTR_4g109540 48.052 77 40 0 12 88 54 130 1.09e-20 87.0
MS.gene45667.t1 MTR_3g084040 50.633 79 38 1 9 87 37 114 1.56e-20 83.6
MS.gene45667.t1 MTR_4g104470 50.617 81 40 0 8 88 216 296 2.11e-20 86.7
MS.gene45667.t1 MTR_4g104470 40.964 83 49 0 8 90 122 204 4.90e-14 68.9
MS.gene45667.t1 MTR_7g013740 43.750 80 45 0 9 88 41 120 5.80e-19 79.3
MS.gene45667.t1 MTR_3g075500 55.385 65 29 0 8 72 206 270 1.59e-18 80.9
MS.gene45667.t1 MTR_3g075500 39.506 81 49 0 9 89 110 190 3.31e-11 60.8
MS.gene45667.t1 MTR_1g064230 50.575 87 43 0 2 88 190 276 3.16e-18 80.5
MS.gene45667.t1 MTR_1g064230 36.283 113 71 1 9 120 85 197 5.10e-11 60.5
MS.gene45667.t1 MTR_3g080130 40.244 82 49 0 9 90 37 118 3.26e-18 77.0
MS.gene45667.t1 MTR_3g014000 40.909 110 46 4 1 103 31 128 2.55e-17 75.1
MS.gene45667.t1 MTR_7g105260 40.741 81 48 0 6 86 30 110 5.01e-17 74.3
MS.gene45667.t1 MTR_3g116130 46.053 76 40 1 9 84 30 104 2.66e-16 75.1
MS.gene45667.t1 MTR_4g035310 41.000 100 52 4 5 99 12 109 2.91e-16 75.1
MS.gene45667.t1 MTR_4g035310 41.000 100 52 4 5 99 12 109 3.72e-16 74.7
MS.gene45667.t1 MTR_1g045510 41.463 82 48 0 4 85 200 281 1.55e-15 73.2
MS.gene45667.t1 MTR_3g055990 37.975 79 47 1 9 87 7 83 1.97e-15 73.9
MS.gene45667.t1 MTR_3g055990 31.126 151 87 4 9 144 107 255 7.75e-11 60.5
MS.gene45667.t1 MTR_3g055990 37.975 79 47 1 9 87 7 83 2.07e-15 73.9
MS.gene45667.t1 MTR_3g055990 31.126 151 87 4 9 144 107 255 8.23e-11 60.5
MS.gene45667.t1 MTR_3g116130 46.053 76 40 1 9 84 30 104 2.13e-15 73.6
MS.gene45667.t1 MTR_3g084040 47.297 74 34 2 1 74 33 101 2.38e-15 68.9
MS.gene45667.t1 MTR_3g092490 30.769 169 92 4 9 153 27 194 2.46e-15 72.0
MS.gene45667.t1 MTR_5g097580 37.975 79 47 1 9 87 7 83 2.53e-15 73.6
MS.gene45667.t1 MTR_5g097580 32.258 93 62 1 9 101 107 198 4.47e-11 61.2
MS.gene45667.t1 MTR_5g097580 37.975 79 47 1 9 87 7 83 2.61e-15 73.6
MS.gene45667.t1 MTR_5g097580 32.258 93 62 1 9 101 107 198 4.10e-11 61.2
MS.gene45667.t1 MTR_7g117495 46.053 76 40 1 9 84 19 93 4.65e-15 71.2
MS.gene45667.t1 MTR_7g117495 46.053 76 40 1 9 84 19 93 6.47e-15 70.9
MS.gene45667.t1 MTR_3g092490 28.485 165 98 3 9 170 27 174 1.07e-14 70.5
MS.gene45667.t1 MTR_2g096560 39.024 82 50 0 9 90 8 89 1.10e-14 66.6
MS.gene45667.t1 MTR_2g026180 39.241 79 46 1 9 87 5 81 1.18e-14 71.6
MS.gene45667.t1 MTR_2g026180 33.607 122 71 3 9 120 107 228 3.43e-11 61.6
MS.gene45667.t1 MTR_5g064120 44.737 76 41 1 9 84 17 91 1.23e-14 70.5
MS.gene45667.t1 MTR_3g092490 45.000 80 43 1 9 88 27 105 1.59e-14 68.6
MS.gene45667.t1 MTR_8g024520 44.737 76 41 1 9 84 26 100 2.12e-14 70.1
MS.gene45667.t1 MTR_8g024520 44.737 76 41 1 9 84 26 100 2.16e-14 70.1
MS.gene45667.t1 MTR_1g073690 35.443 79 49 1 9 87 7 83 2.17e-14 70.9
MS.gene45667.t1 MTR_1g073690 33.117 154 89 5 9 151 113 263 6.87e-12 63.5
MS.gene45667.t1 MTR_4g117350 37.500 96 57 1 1 96 67 159 5.57e-14 66.6
MS.gene45667.t1 MTR_6g004810 38.095 84 50 1 9 92 46 127 1.17e-13 68.6
MS.gene45667.t1 MTR_3g082650 36.585 82 50 1 9 90 19 98 2.36e-13 67.4
MS.gene45667.t1 MTR_3g082650 33.333 138 85 2 9 146 107 237 2.71e-13 67.4
MS.gene45667.t1 MTR_7g099940 37.931 87 54 0 1 87 28 114 4.07e-13 63.9
MS.gene45667.t1 MTR_5g023060 32.386 176 98 6 10 170 133 302 1.18e-12 65.1
MS.gene45667.t1 MTR_6g477870 38.750 80 49 0 9 88 96 175 1.37e-12 63.5
MS.gene45667.t1 MTR_4g081520 38.298 94 52 2 11 101 54 144 2.30e-12 62.0
MS.gene45667.t1 MTR_5g017280 41.250 80 43 2 9 87 5 81 3.13e-12 64.3
MS.gene45667.t1 MTR_5g017280 41.250 80 43 2 9 87 5 81 3.13e-12 64.3
MS.gene45667.t1 MTR_4g117350 40.278 72 40 1 1 72 67 135 6.87e-12 60.5
MS.gene45667.t1 MTR_4g036415 44.444 72 40 0 10 81 453 524 1.05e-11 63.2
MS.gene45667.t1 MTR_4g063560 39.726 73 44 0 9 81 34 106 1.23e-11 59.3
MS.gene45667.t1 MTR_1g092860 35.870 92 56 1 9 97 193 284 1.34e-11 62.0
MS.gene45667.t1 MTR_3g065190 39.175 97 58 1 3 98 107 203 2.29e-11 62.0
MS.gene45667.t1 MTR_2g026180 33.607 122 71 3 9 120 37 158 2.35e-11 62.0
MS.gene45667.t1 MTR_3g103420 29.851 134 88 2 9 137 118 250 2.70e-11 61.6
MS.gene45667.t1 MTR_4g113150 39.837 123 65 6 10 126 9 128 3.90e-11 61.2
MS.gene45667.t1 MTR_5g044970 36.957 92 58 0 10 101 12 103 4.15e-11 59.3
MS.gene45667.t1 MTR_4g113150 39.837 123 65 6 10 126 9 128 4.73e-11 61.2
MS.gene45667.t1 MTR_3g029580 38.356 73 45 0 9 81 33 105 8.66e-11 57.8
MS.gene45667.t1 MTR_8g463890 35.955 89 55 2 9 97 74 160 9.32e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45667.t1 AT4G39260 77.778 90 20 0 4 93 2 91 4.88e-48 152
MS.gene45667.t1 AT2G21660 78.824 85 18 0 1 85 1 85 3.73e-45 147
MS.gene45667.t1 AT2G21660 78.824 85 18 0 1 85 1 85 6.23e-45 147
MS.gene45667.t1 AT4G39260 75.610 82 20 0 4 85 2 83 2.48e-42 140
MS.gene45667.t1 AT4G39260 62.016 129 27 1 4 132 2 108 6.11e-36 122
MS.gene45667.t1 AT4G39260 76.119 67 16 0 4 70 2 68 3.71e-34 117
MS.gene45667.t1 AT3G26420 54.167 96 43 1 3 98 2 96 3.52e-31 114
MS.gene45667.t1 AT4G13850 54.217 83 38 0 9 91 36 118 3.72e-25 95.9
MS.gene45667.t1 AT3G23830 52.475 101 43 1 9 109 36 131 7.31e-24 92.0
MS.gene45667.t1 AT3G23830 52.475 101 43 1 9 109 36 131 7.31e-24 92.0
MS.gene45667.t1 AT5G04280 47.475 99 47 1 9 102 8 106 8.91e-24 95.9
MS.gene45667.t1 AT4G13850 56.566 99 43 0 9 107 36 134 1.42e-23 91.3
MS.gene45667.t1 AT4G13850 57.426 101 43 0 9 109 36 136 2.74e-23 90.9
MS.gene45667.t1 AT4G13850 56.383 94 40 1 9 101 36 129 3.85e-23 89.7
MS.gene45667.t1 AT5G61030 49.367 79 40 0 9 87 41 119 1.91e-22 92.4
MS.gene45667.t1 AT5G61030 49.367 79 40 0 9 87 41 119 1.91e-22 92.4
MS.gene45667.t1 AT2G21690 50.000 90 40 1 3 87 2 91 2.56e-21 84.7
MS.gene45667.t1 AT4G24770 47.561 82 43 0 8 89 244 325 1.54e-20 87.4
MS.gene45667.t1 AT4G24770 45.783 83 45 0 7 89 149 231 8.76e-16 74.3
MS.gene45667.t1 AT4G24770 47.561 82 43 0 8 89 244 325 1.54e-20 87.4
MS.gene45667.t1 AT4G24770 45.783 83 45 0 7 89 149 231 8.76e-16 74.3
MS.gene45667.t1 AT5G06210 44.318 88 49 0 6 93 32 119 1.80e-20 83.6
MS.gene45667.t1 AT5G50250 48.193 83 43 0 5 87 204 286 1.05e-19 84.7
MS.gene45667.t1 AT5G50250 44.578 83 46 0 7 89 112 194 1.89e-15 72.8
MS.gene45667.t1 AT3G53460 45.783 83 45 0 9 91 258 340 1.83e-19 84.7
MS.gene45667.t1 AT3G53460 45.783 83 45 0 9 91 258 340 1.83e-19 84.7
MS.gene45667.t1 AT3G53460 45.783 83 45 0 9 91 258 340 1.83e-19 84.7
MS.gene45667.t1 AT2G37220 45.679 81 44 0 9 89 205 285 2.54e-19 83.6
MS.gene45667.t1 AT3G53460 45.783 83 45 0 9 91 279 361 2.59e-19 84.3
MS.gene45667.t1 AT1G60650 42.169 83 48 0 5 87 9 91 3.74e-19 83.2
MS.gene45667.t1 AT1G60650 42.169 83 48 0 5 87 9 91 3.74e-19 83.2
MS.gene45667.t1 AT1G74230 52.688 93 39 2 1 93 31 118 4.99e-19 82.8
MS.gene45667.t1 AT3G07810 41.772 79 44 1 9 87 7 83 2.40e-17 79.3
MS.gene45667.t1 AT3G07810 32.847 137 73 4 9 130 109 241 1.66e-11 62.4
MS.gene45667.t1 AT3G07810 41.772 79 44 1 9 87 7 83 2.48e-17 79.3
MS.gene45667.t1 AT3G07810 32.847 137 73 4 9 130 109 241 1.63e-11 62.4
MS.gene45667.t1 AT1G76460 48.684 76 38 1 9 84 25 99 4.94e-17 77.4
MS.gene45667.t1 AT1G22330 44.118 102 51 3 9 105 18 118 9.04e-17 76.6
MS.gene45667.t1 AT1G18630 46.154 78 42 0 9 86 37 114 1.37e-16 73.6
MS.gene45667.t1 AT5G47320 46.753 77 41 0 9 85 32 108 1.99e-16 74.3
MS.gene45667.t1 AT4G26650 34.286 105 67 1 9 113 13 115 2.04e-16 76.6
MS.gene45667.t1 AT4G26650 34.286 105 67 1 9 113 16 118 2.09e-16 76.6
MS.gene45667.t1 AT4G14300 40.506 79 45 1 9 87 7 83 2.95e-16 76.3
MS.gene45667.t1 AT4G14300 40.506 79 45 1 9 87 7 83 2.95e-16 76.3
MS.gene45667.t1 AT1G20880 46.053 76 40 1 9 84 25 99 5.92e-16 74.3
MS.gene45667.t1 AT1G20880 46.053 76 40 1 9 84 25 99 5.92e-16 74.3
MS.gene45667.t1 AT1G20880 46.053 76 40 1 9 84 25 99 5.92e-16 74.3
MS.gene45667.t1 AT3G46020 42.857 77 44 0 9 85 8 84 1.70e-15 69.3
MS.gene45667.t1 AT1G22910 40.404 99 54 3 5 99 9 106 1.95e-15 72.4
MS.gene45667.t1 AT2G33410 39.241 79 46 1 9 87 7 83 2.38e-15 73.6
MS.gene45667.t1 AT2G33410 34.177 79 51 1 9 87 111 188 9.37e-11 60.1
MS.gene45667.t1 AT2G46780 46.053 76 40 1 9 84 23 97 2.55e-15 73.2
MS.gene45667.t1 AT2G46780 46.053 76 40 1 9 84 23 97 2.55e-15 73.2
MS.gene45667.t1 AT1G22910 40.404 99 54 3 5 99 9 106 2.67e-15 72.0
MS.gene45667.t1 AT3G13224 38.462 91 50 2 6 92 4 92 3.83e-15 72.8
MS.gene45667.t1 AT3G13224 32.955 88 58 1 3 89 94 181 8.89e-12 63.2
MS.gene45667.t1 AT3G13224 39.286 84 49 1 9 92 20 101 4.42e-15 72.4
MS.gene45667.t1 AT3G13224 32.955 88 58 1 3 89 103 190 2.71e-12 64.7
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 5.45e-15 72.4
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 5.45e-15 72.4
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 5.45e-15 72.4
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 5.45e-15 72.4
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 5.45e-15 72.4
MS.gene45667.t1 AT1G33470 43.421 76 42 1 9 84 8 82 6.02e-15 70.9
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 6.78e-15 72.4
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 6.78e-15 72.4
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 6.78e-15 72.4
MS.gene45667.t1 AT5G55550 33.981 103 66 1 9 111 7 107 6.78e-15 72.4
MS.gene45667.t1 AT1G33470 43.421 76 42 1 9 84 8 82 6.87e-15 70.9
MS.gene45667.t1 AT1G33470 43.421 76 42 1 9 84 8 82 7.32e-15 70.9
MS.gene45667.t1 AT1G17640 42.500 80 44 1 9 88 67 144 1.18e-14 71.2
MS.gene45667.t1 AT1G17640 42.500 80 44 1 9 88 67 144 1.18e-14 71.2
MS.gene45667.t1 AT1G17640 42.500 80 44 1 9 88 67 144 1.18e-14 71.2
MS.gene45667.t1 AT3G08000 43.678 87 43 2 9 92 42 125 1.64e-14 67.8
MS.gene45667.t1 AT1G22910 40.404 99 54 3 5 99 9 106 1.88e-14 70.9
MS.gene45667.t1 AT2G37510 45.946 74 40 0 9 82 35 108 2.29e-14 67.4
MS.gene45667.t1 AT5G59865 44.304 79 44 0 11 89 29 107 3.94e-14 65.9
MS.gene45667.t1 AT1G78260 43.210 81 44 2 5 84 13 92 5.21e-14 68.9
MS.gene45667.t1 AT3G54770 43.421 76 42 1 9 84 18 92 6.25e-14 68.6
MS.gene45667.t1 AT3G54770 43.421 76 42 1 9 84 18 92 7.88e-14 68.2
MS.gene45667.t1 AT1G78260 43.421 76 42 1 9 84 18 92 8.98e-14 68.2
MS.gene45667.t1 AT5G19960 35.897 117 66 2 10 126 9 116 2.40e-13 67.4
MS.gene45667.t1 AT5G53720 42.683 82 46 1 3 84 4 84 2.63e-13 63.5
MS.gene45667.t1 AT5G40490 38.542 96 57 1 9 104 43 136 4.52e-13 67.0
MS.gene45667.t1 AT1G60000 40.244 82 49 0 4 85 173 254 4.71e-13 65.9
MS.gene45667.t1 AT5G55550 40.278 72 41 1 9 80 7 76 5.18e-13 67.0
MS.gene45667.t1 AT2G27330 29.670 91 63 1 11 100 24 114 6.01e-13 62.8
MS.gene45667.t1 AT3G13224 39.286 84 49 1 9 92 20 101 6.13e-13 65.5
MS.gene45667.t1 AT5G55550 40.278 72 41 1 9 80 7 76 6.62e-13 66.6
MS.gene45667.t1 AT3G14100 42.308 78 45 0 7 84 143 220 7.20e-13 66.2
MS.gene45667.t1 AT2G14160 39.286 84 32 2 1 66 1 83 8.22e-13 61.6
MS.gene45667.t1 AT5G47620 36.709 79 48 1 9 87 7 83 8.51e-13 66.2
MS.gene45667.t1 AT5G47620 36.709 79 48 1 9 87 7 83 8.51e-13 66.2
MS.gene45667.t1 AT5G47620 36.709 79 48 1 9 87 7 83 8.51e-13 66.2
MS.gene45667.t1 AT4G13860 40.541 74 44 0 9 82 4 77 1.51e-12 61.2
MS.gene45667.t1 AT1G01080 41.772 79 41 2 7 83 211 286 2.12e-12 64.7
MS.gene45667.t1 AT1G01080 41.772 79 41 2 7 83 212 287 2.13e-12 64.7
MS.gene45667.t1 AT1G54080 42.308 78 45 0 7 84 147 224 3.41e-12 64.3
MS.gene45667.t1 AT3G06970 27.660 141 95 3 9 148 13 147 6.14e-12 63.5
MS.gene45667.t1 AT5G53680 46.377 69 36 1 9 77 14 81 6.19e-12 61.6
MS.gene45667.t1 AT5G51300 30.345 145 91 3 7 150 479 614 8.61e-12 63.5
MS.gene45667.t1 AT5G51300 30.345 145 91 3 7 150 479 614 8.61e-12 63.5
MS.gene45667.t1 AT5G51300 30.345 145 91 3 7 150 479 614 8.61e-12 63.5
MS.gene45667.t1 AT5G04280 47.059 68 31 1 40 102 1 68 1.16e-11 62.4
MS.gene45667.t1 AT5G04280 47.059 68 31 1 40 102 1 68 1.16e-11 62.4
MS.gene45667.t1 AT5G04280 47.059 68 31 1 40 102 1 68 1.16e-11 62.4
MS.gene45667.t1 AT5G04280 47.059 68 31 1 40 102 1 68 1.16e-11 62.4
MS.gene45667.t1 AT5G47620 37.500 72 43 1 9 80 7 76 1.20e-11 62.8
MS.gene45667.t1 AT4G27000 43.243 74 41 1 7 79 172 245 2.13e-11 62.0
MS.gene45667.t1 AT4G36960 36.782 87 53 1 6 90 1 87 4.17e-11 61.2
MS.gene45667.t1 AT4G36960 36.782 87 53 1 6 90 1 87 4.17e-11 61.2
MS.gene45667.t1 AT3G20930 35.000 80 52 0 9 88 283 362 5.20e-11 60.8
MS.gene45667.t1 AT2G22090 38.043 92 54 3 5 95 100 189 8.56e-11 60.1
MS.gene45667.t1 AT2G22090 38.043 92 54 3 5 95 100 189 9.15e-11 60.1

Find 92 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGAAGATCAAGAGGTTT+TGG 0.244628 4.4:-37772103 MS.gene45667:CDS
GGTGGAGGTGGTGGTGGTTA+TGG 0.247285 4.4:-37771812 MS.gene45667:CDS
GGTGGCGGCGGTGGTGGATA+TGG 0.307147 4.4:-37771953 MS.gene45667:CDS
GGTGGTGGCGGTTATGGTGG+AGG 0.307721 4.4:-37771851 MS.gene45667:CDS
TATGGTGGCGGCGGTGGTTA+TGG 0.314627 4.4:-37771935 MS.gene45667:CDS
GGAGGTGGTGGTGGTTATGG+TGG 0.314652 4.4:-37771809 MS.gene45667:CDS
AGTGGTGGTGGTGGCGGTTA+TGG 0.324812 4.4:-37771857 MS.gene45667:CDS
GTTATCAATGATCGTGAAAC+TGG 0.330573 4.4:-37772121 MS.gene45667:CDS
GGTCGAGGTGGCGGCGGTGG+TGG 0.344552 4.4:-37771959 MS.gene45667:CDS
GGAGGTGGTGACCGTGGTTA+TGG 0.350450 4.4:-37771758 MS.gene45667:CDS
GGAGGTCGAGGTGGCGGCGG+TGG 0.351610 4.4:-37771962 MS.gene45667:CDS
GCTTCGTCGGTGGTCTTGCT+TGG 0.353153 4.4:-37772453 MS.gene45667:CDS
GTGCTGATAGCGGTGGAAAC+TGG 0.354817 4.4:-37771697 MS.gene45667:CDS
GAGCAAGCCTTCTCTAAGTT+TGG 0.374142 4.4:-37772409 MS.gene45667:CDS
GTGGTGGCGGTTATGGTGGA+GGG 0.383697 4.4:-37771850 MS.gene45667:CDS
CGTGGATATGGTGGAGGTGG+TGG 0.384415 4.4:-37771821 MS.gene45667:CDS
CTTCGTCGGTGGTCTTGCTT+GGG 0.385202 4.4:-37772452 MS.gene45667:CDS
GGTGGTTATGGCGGAGGTGG+TGG 0.401287 4.4:-37771923 MS.gene45667:CDS
GGTGGTAGAGACCGTGGATA+TGG 0.401669 4.4:-37771785 MS.gene45667:CDS
GGTGGTGGTGGTTATGGTGG+AGG 0.410493 4.4:-37771806 MS.gene45667:CDS
GGCGGAGGTGGTGGTGGCTA+TGG 0.415407 4.4:-37771914 MS.gene45667:CDS
GGTGGTGGTGGCTATGGAGG+TGG 0.416082 4.4:-37771908 MS.gene45667:CDS
CGTGGTTATGGTGGTGGAGG+TGG 0.419917 4.4:-37771746 MS.gene45667:CDS
GGAGGAGGTCGAGGTGGCGG+CGG 0.426267 4.4:-37771965 MS.gene45667:CDS
GGTGGATATGGTGGCGGCGG+TGG 0.432939 4.4:-37771941 MS.gene45667:CDS
GGCGGTGGTTATGGCGGAGG+TGG 0.433064 4.4:-37771926 MS.gene45667:CDS
GGAGGTGGTGGTGGCTATGG+AGG 0.438729 4.4:-37771911 MS.gene45667:CDS
GGATATGGTGGAGGTGGTGG+TGG 0.439317 4.4:-37771818 MS.gene45667:CDS
GGCCACCGATAGCCAAGCTC+TGG 0.439871 4.4:-37772431 MS.gene45667:CDS
GGTTATGGCGGAGGTGGTGG+TGG 0.443104 4.4:-37771920 MS.gene45667:CDS
GGTAGTGGTGGAGGAGGTCG+AGG 0.455825 4.4:-37771974 MS.gene45667:CDS
GGCGGCGGTGGTGGATATGG+TGG 0.462700 4.4:-37771950 MS.gene45667:CDS
GGTGGTGGTGGCGGTTATGG+TGG 0.463200 4.4:-37771854 MS.gene45667:CDS
TGGTGGCGGTTATGGTGGAG+GGG 0.468047 4.4:-37771849 MS.gene45667:CDS
GGTGGCGGTTATGGTGGAGG+GGG 0.468567 4.4:-37771848 MS.gene45667:CDS
GGTGGAGGAGGTCGAGGTGG+CGG 0.471160 4.4:-37771968 MS.gene45667:CDS
GGTTATGGTGGTGGAGGTGG+CGG 0.475016 4.4:-37771743 MS.gene45667:CDS
AGAAGGCTTGCTCCAGAGCT+TGG 0.478074 4.4:+37772419 None:intergenic
GGTGATCGTGGATATGGTGG+AGG 0.478917 4.4:-37771827 MS.gene45667:CDS
CGTGGATATGGTGGTGGAGG+TGG 0.479477 4.4:-37771773 MS.gene45667:CDS
GGCGGCGGTGGTTATGGCGG+AGG 0.479896 4.4:-37771929 MS.gene45667:CDS
AGCAGAAGTGGTGGTGGTGG+CGG 0.484704 4.4:-37771863 MS.gene45667:CDS
GGCGGTGGTGGATATGGTGG+CGG 0.485442 4.4:-37771947 MS.gene45667:CDS
GAAGCTCAGTCCCGTGGTAG+TGG 0.495918 4.4:-37771989 MS.gene45667:CDS
GGTGGTGGTTATGGTGGAGG+TGG 0.498780 4.4:-37771803 MS.gene45667:CDS
CAAGACCACCGACGAAGCAC+CGG 0.514431 4.4:+37772458 None:intergenic
CAGTCCCGTGGTAGTGGTGG+AGG 0.515463 4.4:-37771983 MS.gene45667:CDS
GATCGCTACTCTCGCGGTGG+TGG 0.518871 4.4:-37771719 MS.gene45667:CDS
GATCGTGGATATGGTGGAGG+TGG 0.521221 4.4:-37771824 MS.gene45667:CDS
TCCCGTGGTAGTGGTGGAGG+AGG 0.528218 4.4:-37771980 MS.gene45667:CDS
GGAGGTGGTGGTCGACGTGA+TGG 0.531938 4.4:-37771893 MS.gene45667:CDS
GGTGACCGTGGTTATGGTGG+TGG 0.532821 4.4:-37771752 MS.gene45667:CDS
TACAGCAGAAGTGGTGGTGG+TGG 0.534700 4.4:-37771866 MS.gene45667:CDS
ACCTCCTCCACCACTACCAC+GGG 0.543580 4.4:+37771979 None:intergenic
GACCGTGGTTATGGTGGTGG+AGG 0.549033 4.4:-37771749 MS.gene45667:CDS
AATTGAAGAGATGAACGGTC+AGG 0.551398 4.4:-37772041 MS.gene45667:CDS
GACCGTGGATATGGTGGTGG+AGG 0.551656 4.4:-37771776 MS.gene45667:CDS
TGGTGAAATCACTGATTCCA+AGG 0.553599 4.4:-37772389 MS.gene45667:intron
GGTGGTGGATATGGTGGCGG+CGG 0.554336 4.4:-37771944 MS.gene45667:CDS
GGTGGCGGCGGTGGTTATGG+CGG 0.555099 4.4:-37771932 MS.gene45667:CDS
GGTGGTGGCTATGGAGGTGG+TGG 0.555533 4.4:-37771905 MS.gene45667:CDS
TTGCTCCAGAGCTTGGCTAT+CGG 0.556107 4.4:+37772426 None:intergenic
AGAGACCGTGGATATGGTGG+TGG 0.557634 4.4:-37771779 MS.gene45667:CDS
CGTGAAACTGGAAGATCAAG+AGG 0.562850 4.4:-37772109 MS.gene45667:CDS
GCTCAGTCCCGTGGTAGTGG+TGG 0.563419 4.4:-37771986 MS.gene45667:CDS
GGTGGTGGTCGACGTGATGG+TGG 0.565795 4.4:-37771890 MS.gene45667:CDS
CGCGGTGGTGGTGCTGATAG+CGG 0.567365 4.4:-37771707 MS.gene45667:CDS
GGCGGTGATCGCTACTCTCG+CGG 0.570761 4.4:-37771725 MS.gene45667:CDS
CTCCAGAGCTTGGCTATCGG+TGG 0.571940 4.4:+37772429 None:intergenic
GGTGGTGACCGTGGTTATGG+TGG 0.575688 4.4:-37771755 MS.gene45667:CDS
GATTTCACCAAACTTAGAGA+AGG 0.575852 4.4:+37772402 None:intergenic
GACCTCCTCCACCACTACCA+CGG 0.582027 4.4:+37771978 None:intergenic
GGATACAGCAGAAGTGGTGG+TGG 0.583391 4.4:-37771869 MS.gene45667:CDS
GTTGAGTACCGGTGCTTCGT+CGG 0.588309 4.4:-37772466 MS.gene45667:CDS
AGTGGTGGAGGAGGTCGAGG+TGG 0.601853 4.4:-37771971 MS.gene45667:CDS
CTGATAGCGGTGGAAACTGG+AGG 0.605217 4.4:-37771694 MS.gene45667:CDS
ATGAACGGTCAGGATATTGA+CGG 0.611489 4.4:-37772031 MS.gene45667:CDS
GAGTACCGGTGCTTCGTCGG+TGG 0.614835 4.4:-37772463 MS.gene45667:CDS
ACGGTCAGGATATTGACGGC+AGG 0.621480 4.4:-37772027 MS.gene45667:CDS
GTTCTTCTGATGTTGAGTAC+CGG 0.622558 4.4:-37772477 MS.gene45667:CDS
GGTGGTGGAGGTGGTGACCG+TGG 0.625086 4.4:-37771764 MS.gene45667:CDS
CACCTCCACCACCATATCCA+CGG 0.628164 4.4:+37771774 None:intergenic
GGTAGAGACCGTGGATATGG+TGG 0.628309 4.4:-37771782 MS.gene45667:CDS
TGATAGCGGTGGAAACTGGA+GGG 0.650756 4.4:-37771693 MS.gene45667:CDS
GTGAATGAAGCTCAGTCCCG+TGG 0.655571 4.4:-37771995 MS.gene45667:CDS
GGTGGAGGTGGTAGAGACCG+TGG 0.658126 4.4:-37771791 MS.gene45667:CDS
GATGCAATTGAAGAGATGAA+CGG 0.671848 4.4:-37772046 MS.gene45667:CDS
GATGGTGGATACAGCAGAAG+TGG 0.672149 4.4:-37771875 MS.gene45667:CDS
CACCTCCACCACCATAACCA+CGG 0.677931 4.4:+37771747 None:intergenic
GGTGATCGCTACTCTCGCGG+TGG 0.687217 4.4:-37771722 MS.gene45667:CDS
GGTGGATACAGCAGAAGTGG+TGG 0.687566 4.4:-37771872 MS.gene45667:CDS
GGTGGTGGTGCTGATAGCGG+TGG 0.706620 4.4:-37771704 MS.gene45667:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGTGAATTTTCTTAGATCTG+AGG - chr4.4:37772011-37772030 MS.gene45667:CDS 30.0%
!!! ATCTGAGGTTATTTTTGTTC+AGG - chr4.4:37772026-37772045 MS.gene45667:CDS 30.0%
ACCAAAAGAAGAAGATCGAT+CGG - chr4.4:37771951-37771970 MS.gene45667:CDS 35.0%
ATGAAGATGAACGTACCTAT+TGG + chr4.4:37771925-37771944 None:intergenic 35.0%
GATGCAATTGAAGAGATGAA+CGG - chr4.4:37772123-37772142 MS.gene45667:CDS 35.0%
GATTTCACCAAACTTAGAGA+AGG + chr4.4:37771770-37771789 None:intergenic 35.0%
GTTATCAATGATCGTGAAAC+TGG - chr4.4:37772048-37772067 MS.gene45667:CDS 35.0%
! AAGATCGATCGGTTTCTATT+CGG - chr4.4:37771962-37771981 MS.gene45667:CDS 35.0%
! CTTTTGTTACTCACACCAAT+AGG - chr4.4:37771907-37771926 MS.gene45667:CDS 35.0%
AATTGAAGAGATGAACGGTC+AGG - chr4.4:37772128-37772147 MS.gene45667:intron 40.0%
ATGAACGGTCAGGATATTGA+CGG - chr4.4:37772138-37772157 MS.gene45667:intron 40.0%
GTTCTTCTGATGTTGAGTAC+CGG - chr4.4:37771692-37771711 MS.gene45667:CDS 40.0%
! ACCGATCGATCTTCTTCTTT+TGG + chr4.4:37771955-37771974 None:intergenic 40.0%
! ACTGGAAGATCAAGAGGTTT+TGG - chr4.4:37772066-37772085 MS.gene45667:CDS 40.0%
!! GATCGGTTTCTATTCGGTTT+TGG - chr4.4:37771968-37771987 MS.gene45667:CDS 40.0%
!! TGGTGAAATCACTGATTCCA+AGG - chr4.4:37771780-37771799 MS.gene45667:CDS 40.0%
AATCCGAATCAGAATCCGCA+CGG + chr4.4:37771807-37771826 None:intergenic 45.0%
CGTGAAACTGGAAGATCAAG+AGG - chr4.4:37772060-37772079 MS.gene45667:CDS 45.0%
GAGCAAGCCTTCTCTAAGTT+TGG - chr4.4:37771760-37771779 MS.gene45667:CDS 45.0%
TTGCTCCAGAGCTTGGCTAT+CGG + chr4.4:37771746-37771765 None:intergenic 50.0%
!! GATGGTGGATACAGCAGAAG+TGG - chr4.4:37772294-37772313 MS.gene45667:intron 50.0%
!! TGATAGCGGTGGAAACTGGA+GGG - chr4.4:37772476-37772495 MS.gene45667:CDS 50.0%
ACGGTCAGGATATTGACGGC+AGG - chr4.4:37772142-37772161 MS.gene45667:intron 55.0%
AGAGACCGTGGATATGGTGG+TGG - chr4.4:37772390-37772409 MS.gene45667:CDS 55.0%
ATCAGAATCCGCACGGACCT+TGG + chr4.4:37771800-37771819 None:intergenic 55.0%
CACCTCCACCACCATAACCA+CGG + chr4.4:37772425-37772444 None:intergenic 55.0%
CACCTCCACCACCATATCCA+CGG + chr4.4:37772398-37772417 None:intergenic 55.0%
GGATACAGCAGAAGTGGTGG+TGG - chr4.4:37772300-37772319 MS.gene45667:intron 55.0%
GGTAGAGACCGTGGATATGG+TGG - chr4.4:37772387-37772406 MS.gene45667:intron 55.0%
GGTCCGTGCGGATTCTGATT+CGG - chr4.4:37771801-37771820 MS.gene45667:CDS 55.0%
GGTGATCGTGGATATGGTGG+AGG - chr4.4:37772342-37772361 MS.gene45667:intron 55.0%
GGTGGATACAGCAGAAGTGG+TGG - chr4.4:37772297-37772316 MS.gene45667:intron 55.0%
GGTGGTAGAGACCGTGGATA+TGG - chr4.4:37772384-37772403 MS.gene45667:intron 55.0%
GTGAATGAAGCTCAGTCCCG+TGG - chr4.4:37772174-37772193 MS.gene45667:intron 55.0%
! AGAAGGCTTGCTCCAGAGCT+TGG + chr4.4:37771753-37771772 None:intergenic 55.0%
! CTTCGTCGGTGGTCTTGCTT+GGG - chr4.4:37771717-37771736 MS.gene45667:CDS 55.0%
! GATCGTGGATATGGTGGAGG+TGG - chr4.4:37772345-37772364 MS.gene45667:intron 55.0%
!! CACTGATTCCAAGGTCCGTG+CGG - chr4.4:37771789-37771808 MS.gene45667:CDS 55.0%
!! CTGATAGCGGTGGAAACTGG+AGG - chr4.4:37772475-37772494 MS.gene45667:CDS 55.0%
!! GTGCTGATAGCGGTGGAAAC+TGG - chr4.4:37772472-37772491 MS.gene45667:CDS 55.0%
!! GTTGAGTACCGGTGCTTCGT+CGG - chr4.4:37771703-37771722 MS.gene45667:CDS 55.0%
!! TACAGCAGAAGTGGTGGTGG+TGG - chr4.4:37772303-37772322 MS.gene45667:intron 55.0%
ACCTCCTCCACCACTACCAC+GGG + chr4.4:37772193-37772212 None:intergenic 60.0%
CAAGACCACCGACGAAGCAC+CGG + chr4.4:37771714-37771733 None:intergenic 60.0%
CTCCAGAGCTTGGCTATCGG+TGG + chr4.4:37771743-37771762 None:intergenic 60.0%
GAAGCTCAGTCCCGTGGTAG+TGG - chr4.4:37772180-37772199 MS.gene45667:intron 60.0%
GACCTCCTCCACCACTACCA+CGG + chr4.4:37772194-37772213 None:intergenic 60.0%
GGAGGGGGTGATCGTGGATA+TGG - chr4.4:37772336-37772355 MS.gene45667:intron 60.0%
GGAGGTGGTGACCGTGGTTA+TGG - chr4.4:37772411-37772430 MS.gene45667:CDS 60.0%
GGGGGTGATCGTGGATATGG+TGG - chr4.4:37772339-37772358 MS.gene45667:intron 60.0%
GGTGACCGTGGTTATGGTGG+TGG - chr4.4:37772417-37772436 MS.gene45667:CDS 60.0%
GGTGGTGACCGTGGTTATGG+TGG - chr4.4:37772414-37772433 MS.gene45667:CDS 60.0%
! CGTGGATATGGTGGAGGTGG+TGG - chr4.4:37772348-37772367 MS.gene45667:intron 60.0%
! GCTTCGTCGGTGGTCTTGCT+TGG - chr4.4:37771716-37771735 MS.gene45667:CDS 60.0%
! GGATATGGTGGAGGTGGTGG+TGG - chr4.4:37772351-37772370 MS.gene45667:intron 60.0%
! GGTGGTGGTTATGGTGGAGG+TGG - chr4.4:37772366-37772385 MS.gene45667:intron 60.0%
! TATGGTGGAGGGGGTGATCG+TGG - chr4.4:37772330-37772349 MS.gene45667:intron 60.0%
!! AGCAGAAGTGGTGGTGGTGG+CGG - chr4.4:37772306-37772325 MS.gene45667:intron 60.0%
!! AGTGGTGGTGGTGGCGGTTA+TGG - chr4.4:37772312-37772331 MS.gene45667:intron 60.0%
!! CGTGGATATGGTGGTGGAGG+TGG - chr4.4:37772396-37772415 MS.gene45667:CDS 60.0%
!! CGTGGTTATGGTGGTGGAGG+TGG - chr4.4:37772423-37772442 MS.gene45667:CDS 60.0%
!! GACCGTGGATATGGTGGTGG+AGG - chr4.4:37772393-37772412 MS.gene45667:CDS 60.0%
!! GACCGTGGTTATGGTGGTGG+AGG - chr4.4:37772420-37772439 MS.gene45667:CDS 60.0%
!! GGAGGTGGTGGTGGTTATGG+TGG - chr4.4:37772360-37772379 MS.gene45667:intron 60.0%
!! GGTGGAGGTGGTGGTGGTTA+TGG - chr4.4:37772357-37772376 MS.gene45667:intron 60.0%
!! GGTGGTGGTGGTTATGGTGG+AGG - chr4.4:37772363-37772382 MS.gene45667:intron 60.0%
!! GGTTATGGTGGTGGAGGTGG+CGG - chr4.4:37772426-37772445 MS.gene45667:CDS 60.0%
!! GTGGTGGCGGTTATGGTGGA+GGG - chr4.4:37772319-37772338 MS.gene45667:intron 60.0%
!! TATGGTGGCGGCGGTGGTTA+TGG - chr4.4:37772234-37772253 MS.gene45667:intron 60.0%
!! TGGTGGCGGTTATGGTGGAG+GGG - chr4.4:37772320-37772339 MS.gene45667:intron 60.0%
GATCGCTACTCTCGCGGTGG+TGG - chr4.4:37772450-37772469 MS.gene45667:CDS 65.0%
GGCCACCGATAGCCAAGCTC+TGG - chr4.4:37771738-37771757 MS.gene45667:CDS 65.0%
GGCGGTGATCGCTACTCTCG+CGG - chr4.4:37772444-37772463 MS.gene45667:CDS 65.0%
GGTGATCGCTACTCTCGCGG+TGG - chr4.4:37772447-37772466 MS.gene45667:CDS 65.0%
GGTGGAGGTGGTAGAGACCG+TGG - chr4.4:37772378-37772397 MS.gene45667:intron 65.0%
! AGTGGTGGAGGAGGTCGAGG+TGG - chr4.4:37772198-37772217 MS.gene45667:intron 65.0%
! GGAGGTGGTGGTCGACGTGA+TGG - chr4.4:37772276-37772295 MS.gene45667:intron 65.0%
! GGTAGTGGTGGAGGAGGTCG+AGG - chr4.4:37772195-37772214 MS.gene45667:intron 65.0%
! GGTGGCGGTTATGGTGGAGG+GGG - chr4.4:37772321-37772340 MS.gene45667:intron 65.0%
! GGTGGTGGCTATGGAGGTGG+TGG - chr4.4:37772264-37772283 MS.gene45667:intron 65.0%
! GGTGGTGGTCGACGTGATGG+TGG - chr4.4:37772279-37772298 MS.gene45667:intron 65.0%
! GGTGGTTATGGCGGAGGTGG+TGG - chr4.4:37772246-37772265 MS.gene45667:intron 65.0%
! GGTTATGGCGGAGGTGGTGG+TGG - chr4.4:37772249-37772268 MS.gene45667:intron 65.0%
!! CAGTCCCGTGGTAGTGGTGG+AGG - chr4.4:37772186-37772205 MS.gene45667:intron 65.0%
!! CGCGGTGGTGGTGCTGATAG+CGG - chr4.4:37772462-37772481 MS.gene45667:CDS 65.0%
!! GAGTACCGGTGCTTCGTCGG+TGG - chr4.4:37771706-37771725 MS.gene45667:CDS 65.0%
!! GCTCAGTCCCGTGGTAGTGG+TGG - chr4.4:37772183-37772202 MS.gene45667:intron 65.0%
!! GGAGGTGGTGGTGGCTATGG+AGG - chr4.4:37772258-37772277 MS.gene45667:intron 65.0%
!! GGCGGTGGTGGATATGGTGG+CGG - chr4.4:37772222-37772241 MS.gene45667:intron 65.0%
!! GGTGGTGGATATGGTGGCGG+CGG - chr4.4:37772225-37772244 MS.gene45667:intron 65.0%
!! GGTGGTGGCGGTTATGGTGG+AGG - chr4.4:37772318-37772337 MS.gene45667:intron 65.0%
!! GGTGGTGGTGCTGATAGCGG+TGG - chr4.4:37772465-37772484 MS.gene45667:CDS 65.0%
!! GGTGGTGGTGGCGGTTATGG+TGG - chr4.4:37772315-37772334 MS.gene45667:intron 65.0%
!! GGTGGTGGTGGCTATGGAGG+TGG - chr4.4:37772261-37772280 MS.gene45667:intron 65.0%
!! TCCCGTGGTAGTGGTGGAGG+AGG - chr4.4:37772189-37772208 MS.gene45667:intron 65.0%
GGTGGAGGAGGTCGAGGTGG+CGG - chr4.4:37772201-37772220 MS.gene45667:intron 70.0%
! GGCGGTGGTTATGGCGGAGG+TGG - chr4.4:37772243-37772262 MS.gene45667:intron 70.0%
! GGTGGCGGCGGTGGTTATGG+CGG - chr4.4:37772237-37772256 MS.gene45667:intron 70.0%
!! GGCGGAGGTGGTGGTGGCTA+TGG - chr4.4:37772255-37772274 MS.gene45667:intron 70.0%
!! GGCGGCGGTGGTGGATATGG+TGG - chr4.4:37772219-37772238 MS.gene45667:intron 70.0%
!! GGTGGATATGGTGGCGGCGG+TGG - chr4.4:37772228-37772247 MS.gene45667:intron 70.0%
!! GGTGGCGGCGGTGGTGGATA+TGG - chr4.4:37772216-37772235 MS.gene45667:intron 70.0%
!! GGTGGTGGAGGTGGTGACCG+TGG - chr4.4:37772405-37772424 MS.gene45667:CDS 70.0%
GGCGGCGGTGGTTATGGCGG+AGG - chr4.4:37772240-37772259 MS.gene45667:intron 75.0%
! GGAGGAGGTCGAGGTGGCGG+CGG - chr4.4:37772204-37772223 MS.gene45667:intron 75.0%
!! GGAGGTCGAGGTGGCGGCGG+TGG - chr4.4:37772207-37772226 MS.gene45667:intron 80.0%
!! GGTCGAGGTGGCGGCGGTGG+TGG - chr4.4:37772210-37772229 MS.gene45667:intron 80.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 37771688 37772503 37771688 ID=MS.gene45667
chr4.4 mRNA 37771688 37772503 37771688 ID=MS.gene45667.t1;Parent=MS.gene45667
chr4.4 exon 37772390 37772503 37772390 ID=MS.gene45667.t1.exon1;Parent=MS.gene45667.t1
chr4.4 CDS 37772390 37772503 37772390 ID=cds.MS.gene45667.t1;Parent=MS.gene45667.t1
chr4.4 exon 37771688 37772143 37771688 ID=MS.gene45667.t1.exon2;Parent=MS.gene45667.t1
chr4.4 CDS 37771688 37772143 37771688 ID=cds.MS.gene45667.t1;Parent=MS.gene45667.t1
Gene Sequence

>MS.gene45667

ATGGGTTCTTCTGATGTTGAGTACCGGTGCTTCGTCGGTGGTCTTGCTTGGGCCACCGATAGCCAAGCTCTGGAGCAAGCCTTCTCTAAGTTTGGTGAAATCACTGATTCCAAGGTCCGTGCGGATTCTGATTCGGATTTCGATTCTGATCTGTGATCTGTTCTGTTACTCTGTTACTTGATCTGTTTGTTACTGATTCTGTTACTATATCATTTGATTCTTTTGTTACTCACACCAATAGGTACGTTCATCTTCATCTTTTTACCAAAAGAAGAAGATCGATCGGTTTCTATTCGGTTTTGGAATTATTGAATTATTTTTCATGTGAATTTTCTTAGATCTGAGGTTATTTTTGTTCAGGTTATCAATGATCGTGAAACTGGAAGATCAAGAGGTTTTGGATTTGTGACATTCGCTGATGAGAAATCGATGAGAGATGCAATTGAAGAGATGAACGGTCAGGATATTGACGGCAGGAATATAACTGTGAATGAAGCTCAGTCCCGTGGTAGTGGTGGAGGAGGTCGAGGTGGCGGCGGTGGTGGATATGGTGGCGGCGGTGGTTATGGCGGAGGTGGTGGTGGCTATGGAGGTGGTGGTCGACGTGATGGTGGATACAGCAGAAGTGGTGGTGGTGGCGGTTATGGTGGAGGGGGTGATCGTGGATATGGTGGAGGTGGTGGTGGTTATGGTGGAGGTGGTAGAGACCGTGGATATGGTGGTGGAGGTGGTGACCGTGGTTATGGTGGTGGAGGTGGCGGTGATCGCTACTCTCGCGGTGGTGGTGCTGATAGCGGTGGAAACTGGAGGGATTAG
Protein sequence

>MS.gene45667.t1

MGSSDVEYRCFVGGLAWATDSQALEQAFSKFGEITDSKVINDRETGRSRGFGFVTFADEKSMRDAIEEMNGQDIDGRNITVNEAQSRGSGGGGRGGGGGGYGGGGGYGGGGGGYGGGGRRDGGYSRSGGGGGYGGGGDRGYGGGGGGYGGGGRDRGYGGGGGDRGYGGGGGGDRYSRGGGADSGGNWRD