AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45670.t1 MTR_4g070080 97.333 75 2 0 1 75 1 75 2.49e-51 158
MS.gene45670.t1 MTR_4g070080 97.333 75 2 0 1 75 1 75 2.42e-50 154
MS.gene45670.t1 MTR_4g070190 89.189 74 8 0 2 75 4 77 1.27e-44 141
MS.gene45670.t1 MTR_4g070140 82.432 74 13 0 2 75 4 77 1.06e-40 132
MS.gene45670.t1 MTR_3g075500 57.353 68 29 0 7 74 207 274 1.30e-23 90.5
MS.gene45670.t1 MTR_4g109540 53.623 69 32 0 7 75 43 111 1.66e-22 87.4
MS.gene45670.t1 MTR_4g104470 54.412 68 31 0 7 74 217 284 3.38e-22 87.0
MS.gene45670.t1 MTR_4g104470 36.111 72 46 0 5 76 121 192 6.74e-11 56.2
MS.gene45670.t1 MTR_3g075500 57.812 64 27 0 7 70 207 270 3.68e-22 86.3
MS.gene45670.t1 MTR_7g013730 50.000 70 35 0 7 76 41 110 5.26e-22 85.9
MS.gene45670.t1 MTR_3g084040 50.725 69 33 1 7 75 37 104 4.88e-21 80.9
MS.gene45670.t1 MTR_4g109540 54.545 66 30 0 10 75 54 119 1.02e-20 82.8
MS.gene45670.t1 MTR_7g013740 45.588 68 37 0 7 74 41 108 3.23e-20 78.6
MS.gene45670.t1 MTR_1g064230 49.315 73 37 0 2 74 192 264 3.84e-20 81.3
MS.gene45670.t1 MTR_3g025880 50.685 73 35 1 7 78 45 117 1.65e-19 80.9
MS.gene45670.t1 MTR_3g084040 50.000 64 31 1 7 70 37 99 4.63e-19 74.7
MS.gene45670.t1 MTR_3g080130 45.588 68 37 0 7 74 37 104 9.27e-19 74.7
MS.gene45670.t1 MTR_7g105260 43.836 73 41 0 4 76 30 102 2.38e-18 73.6
MS.gene45670.t1 MTR_3g014000 50.685 73 25 2 9 74 37 105 9.81e-18 72.0
MS.gene45670.t1 MTR_1g045510 40.278 72 43 0 3 74 201 272 4.39e-17 73.2
MS.gene45670.t1 MTR_7g117495 50.847 59 29 0 7 65 19 77 4.11e-16 70.1
MS.gene45670.t1 MTR_7g117495 50.847 59 29 0 7 65 19 77 4.25e-16 70.1
MS.gene45670.t1 MTR_3g092490 45.455 77 36 3 1 74 20 93 7.19e-16 68.2
MS.gene45670.t1 MTR_3g092490 49.254 67 30 2 1 66 20 83 8.21e-16 69.3
MS.gene45670.t1 MTR_3g092490 49.254 67 30 2 1 66 20 83 8.31e-16 69.3
MS.gene45670.t1 MTR_4g035310 43.243 74 37 2 2 70 11 84 2.63e-15 68.2
MS.gene45670.t1 MTR_4g035310 43.243 74 37 2 2 70 11 84 2.76e-15 68.2
MS.gene45670.t1 MTR_3g029580 40.789 76 45 0 1 76 27 102 3.23e-15 65.5
MS.gene45670.t1 MTR_4g081520 39.474 76 46 0 1 76 46 121 4.56e-15 65.5
MS.gene45670.t1 MTR_6g477870 40.000 70 42 0 7 76 96 165 5.60e-15 65.9
MS.gene45670.t1 MTR_7g099940 40.000 70 42 0 7 76 36 105 7.34e-15 64.7
MS.gene45670.t1 MTR_3g116130 42.857 63 35 1 2 63 24 86 1.31e-14 66.2
MS.gene45670.t1 MTR_5g064120 47.368 57 30 0 7 63 17 73 1.83e-14 65.9
MS.gene45670.t1 MTR_3g116130 42.857 63 35 1 2 63 24 86 1.97e-14 66.2
MS.gene45670.t1 MTR_8g024520 47.170 53 28 0 7 59 26 78 6.02e-14 64.7
MS.gene45670.t1 MTR_8g024520 47.170 53 28 0 7 59 26 78 6.11e-14 64.7
MS.gene45670.t1 MTR_4g036415 46.970 66 35 0 9 74 454 519 7.85e-14 64.7
MS.gene45670.t1 MTR_3g029580 40.000 70 42 0 1 70 27 96 1.21e-13 61.6
MS.gene45670.t1 MTR_7g106440 38.889 72 44 0 3 74 209 280 1.35e-13 63.9
MS.gene45670.t1 MTR_4g063560 36.765 68 43 0 7 74 34 101 1.45e-13 60.8
MS.gene45670.t1 MTR_3g055990 47.059 51 27 0 7 57 7 57 4.11e-13 62.8
MS.gene45670.t1 MTR_3g055990 47.059 51 27 0 7 57 7 57 4.53e-13 62.8
MS.gene45670.t1 MTR_1g080150 34.848 66 43 0 5 70 131 196 6.40e-13 61.6
MS.gene45670.t1 MTR_2g096560 35.714 70 45 0 7 76 8 77 7.18e-13 58.5
MS.gene45670.t1 MTR_3g082650 47.458 59 31 0 7 65 19 77 9.70e-13 61.6
MS.gene45670.t1 MTR_3g082650 40.299 67 40 0 7 73 107 173 5.03e-11 56.6
MS.gene45670.t1 MTR_5g017280 41.429 70 38 1 5 74 3 69 1.86e-12 60.8
MS.gene45670.t1 MTR_6g477870 37.500 64 40 0 7 70 96 159 1.98e-12 58.9
MS.gene45670.t1 MTR_5g017280 41.429 70 38 1 5 74 3 69 2.01e-12 60.8
MS.gene45670.t1 MTR_2g026180 48.000 50 26 0 7 56 5 54 3.13e-12 60.5
MS.gene45670.t1 MTR_7g102730 47.541 61 31 1 9 69 101 160 4.34e-12 60.1
MS.gene45670.t1 MTR_3g065190 45.070 71 38 1 3 72 109 179 4.74e-12 59.7
MS.gene45670.t1 MTR_7g088140 42.373 59 34 0 7 65 128 186 6.15e-12 59.3
MS.gene45670.t1 MTR_7g088140 42.373 59 34 0 7 65 128 186 6.15e-12 59.3
MS.gene45670.t1 MTR_1g073690 33.898 59 39 0 7 65 7 65 7.10e-12 59.3
MS.gene45670.t1 MTR_1g056520 33.333 72 48 0 3 74 204 275 1.02e-11 58.5
MS.gene45670.t1 MTR_1g092860 35.294 68 44 0 7 74 193 260 1.19e-11 58.5
MS.gene45670.t1 MTR_4g117350 36.765 68 43 0 7 74 72 139 1.75e-11 56.6
MS.gene45670.t1 MTR_4g117350 37.500 64 40 0 7 70 72 135 1.75e-11 56.2
MS.gene45670.t1 MTR_5g097580 50.000 48 24 0 7 54 7 54 3.11e-11 57.4
MS.gene45670.t1 MTR_5g097580 50.000 48 24 0 7 54 7 54 3.60e-11 57.4
MS.gene45670.t1 MTR_6g004810 47.458 59 31 0 7 65 46 104 3.84e-11 57.0
MS.gene45670.t1 MTR_6g004810 39.706 68 41 0 7 74 134 201 8.31e-11 56.2
MS.gene45670.t1 MTR_3g027140 36.986 73 46 0 3 75 156 228 9.89e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45670.t1 AT2G21660 82.192 73 13 0 3 75 5 77 1.79e-40 130
MS.gene45670.t1 AT2G21660 82.192 73 13 0 3 75 5 77 2.93e-40 130
MS.gene45670.t1 AT4G39260 71.053 76 22 0 1 76 1 76 1.71e-38 123
MS.gene45670.t1 AT4G39260 71.053 76 22 0 1 76 1 76 3.61e-38 123
MS.gene45670.t1 AT4G39260 71.053 76 22 0 1 76 1 76 1.26e-37 123
MS.gene45670.t1 AT4G39260 72.059 68 19 0 1 68 1 68 5.40e-34 112
MS.gene45670.t1 AT3G26420 56.757 74 32 0 3 76 4 77 1.53e-26 97.4
MS.gene45670.t1 AT3G23830 53.623 69 32 0 7 75 36 104 1.59e-24 89.4
MS.gene45670.t1 AT3G23830 53.623 69 32 0 7 75 36 104 1.59e-24 89.4
MS.gene45670.t1 AT5G61030 54.286 70 32 0 7 76 41 110 5.67e-24 92.0
MS.gene45670.t1 AT5G61030 54.286 70 32 0 7 76 41 110 5.67e-24 92.0
MS.gene45670.t1 AT5G50250 51.389 72 35 0 3 74 204 275 1.20e-22 88.2
MS.gene45670.t1 AT4G13850 50.000 72 36 0 4 75 33 104 3.71e-22 83.2
MS.gene45670.t1 AT4G13850 50.000 72 36 0 4 75 33 104 4.36e-22 83.6
MS.gene45670.t1 AT4G13850 50.000 72 36 0 4 75 33 104 5.45e-22 83.6
MS.gene45670.t1 AT4G13850 50.000 72 36 0 4 75 33 104 6.14e-22 83.6
MS.gene45670.t1 AT2G21690 55.714 70 31 0 2 71 3 72 2.71e-21 80.5
MS.gene45670.t1 AT4G24770 50.725 69 34 0 6 74 244 312 1.42e-20 83.2
MS.gene45670.t1 AT4G24770 40.278 72 43 0 5 76 149 220 7.52e-11 56.2
MS.gene45670.t1 AT4G24770 50.725 69 34 0 6 74 244 312 1.42e-20 83.2
MS.gene45670.t1 AT4G24770 40.278 72 43 0 5 76 149 220 7.52e-11 56.2
MS.gene45670.t1 AT1G74230 55.072 69 30 1 7 75 35 102 4.74e-20 81.3
MS.gene45670.t1 AT3G53460 47.059 68 36 0 7 74 279 346 4.81e-19 79.3
MS.gene45670.t1 AT3G53460 47.059 68 36 0 7 74 258 325 5.98e-19 79.0
MS.gene45670.t1 AT3G53460 47.059 68 36 0 7 74 258 325 5.98e-19 79.0
MS.gene45670.t1 AT3G53460 47.059 68 36 0 7 74 258 325 5.98e-19 79.0
MS.gene45670.t1 AT2G37220 47.059 68 36 0 7 74 205 272 6.57e-19 78.2
MS.gene45670.t1 AT1G18630 47.143 70 37 0 7 76 37 106 6.58e-19 75.5
MS.gene45670.t1 AT5G04280 50.000 66 33 0 7 72 8 73 2.88e-18 76.6
MS.gene45670.t1 AT5G47320 45.588 68 37 0 7 74 32 99 1.21e-17 73.6
MS.gene45670.t1 AT2G37510 47.143 70 37 0 7 76 35 104 1.30e-17 72.0
MS.gene45670.t1 AT4G13860 44.928 69 38 0 7 75 4 72 2.38e-17 69.7
MS.gene45670.t1 AT2G46780 48.387 62 32 0 7 68 23 84 6.03e-17 73.2
MS.gene45670.t1 AT2G46780 48.387 62 32 0 7 68 23 84 6.03e-17 73.2
MS.gene45670.t1 AT3G08000 46.377 69 37 0 7 75 42 110 8.62e-17 69.7
MS.gene45670.t1 AT1G60650 44.000 75 42 0 2 76 8 82 8.91e-17 72.4
MS.gene45670.t1 AT1G60650 44.000 75 42 0 2 76 8 82 8.91e-17 72.4
MS.gene45670.t1 AT5G06210 42.466 73 42 0 4 76 32 104 9.11e-17 69.7
MS.gene45670.t1 AT1G60000 37.500 72 45 0 3 74 174 245 3.90e-16 70.5
MS.gene45670.t1 AT1G78260 45.588 68 36 1 2 68 12 79 9.02e-16 69.3
MS.gene45670.t1 AT1G22330 46.774 62 33 0 7 68 18 79 1.01e-15 69.7
MS.gene45670.t1 AT1G78260 45.588 68 36 1 2 68 12 79 1.04e-15 69.3
MS.gene45670.t1 AT1G76460 49.123 57 29 0 7 63 25 81 1.64e-15 68.9
MS.gene45670.t1 AT1G20880 47.368 57 30 0 7 63 25 81 2.27e-15 68.6
MS.gene45670.t1 AT1G20880 47.368 57 30 0 7 63 25 81 2.27e-15 68.6
MS.gene45670.t1 AT1G20880 47.368 57 30 0 7 63 25 81 2.27e-15 68.6
MS.gene45670.t1 AT1G22910 41.333 75 39 2 1 70 7 81 2.57e-15 67.8
MS.gene45670.t1 AT1G22910 41.333 75 39 2 1 70 7 81 2.78e-15 67.8
MS.gene45670.t1 AT1G22910 41.333 75 39 2 1 70 7 81 5.53e-15 67.8
MS.gene45670.t1 AT5G54580 41.333 75 44 0 2 76 52 126 6.38e-15 65.5
MS.gene45670.t1 AT3G13224 44.286 70 36 1 7 73 20 89 2.07e-14 65.5
MS.gene45670.t1 AT3G13224 39.394 66 40 0 7 72 110 175 1.10e-11 58.2
MS.gene45670.t1 AT1G33470 43.750 64 35 1 1 63 1 64 2.40e-14 65.5
MS.gene45670.t1 AT1G33470 43.750 64 35 1 1 63 1 64 2.48e-14 65.5
MS.gene45670.t1 AT1G33470 43.750 64 35 1 1 63 1 64 2.50e-14 65.5
MS.gene45670.t1 AT5G11412 44.615 65 35 1 2 65 47 111 2.74e-14 63.9
MS.gene45670.t1 AT5G51300 42.857 70 40 0 5 74 479 548 9.72e-14 64.7
MS.gene45670.t1 AT5G51300 42.857 70 40 0 5 74 479 548 9.72e-14 64.7
MS.gene45670.t1 AT5G51300 42.857 70 40 0 5 74 479 548 9.72e-14 64.7
MS.gene45670.t1 AT3G54770 47.458 59 31 0 7 65 18 76 9.75e-14 63.9
MS.gene45670.t1 AT3G54770 47.458 59 31 0 7 65 18 76 9.84e-14 63.9
MS.gene45670.t1 AT3G13224 44.286 70 36 1 7 73 11 80 1.12e-13 64.3
MS.gene45670.t1 AT3G13224 39.394 66 40 0 7 72 101 166 1.52e-11 58.2
MS.gene45670.t1 AT3G46020 34.247 73 48 0 4 76 5 77 1.76e-13 60.5
MS.gene45670.t1 AT2G27330 35.714 70 45 0 9 78 24 93 2.11e-13 60.5
MS.gene45670.t1 AT3G13224 44.286 70 36 1 7 73 20 89 3.68e-13 62.8
MS.gene45670.t1 AT3G13224 39.394 66 40 0 7 72 110 175 3.83e-11 57.4
MS.gene45670.t1 AT5G53720 43.056 72 40 1 3 74 6 76 3.79e-13 59.3
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 4.03e-13 62.8
MS.gene45670.t1 AT1G01080 36.486 74 46 1 1 74 207 279 4.07e-13 62.4
MS.gene45670.t1 AT1G01080 36.486 74 46 1 1 74 208 280 4.09e-13 62.4
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 4.57e-13 62.8
MS.gene45670.t1 AT5G59865 40.580 69 41 0 7 75 27 95 5.23e-13 59.3
MS.gene45670.t1 AT5G19960 43.939 66 36 1 9 74 10 74 5.65e-13 62.4
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 5.80e-13 62.4
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 5.80e-13 62.4
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 5.80e-13 62.4
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 5.80e-13 62.4
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene45670.t1 AT5G55550 50.000 50 25 0 7 56 7 56 6.97e-13 62.0
MS.gene45670.t1 AT3G04500 37.879 66 41 0 5 70 136 201 1.12e-12 60.8
MS.gene45670.t1 AT4G26650 41.176 68 38 1 7 74 16 81 1.51e-12 61.2
MS.gene45670.t1 AT4G13860 46.296 54 29 0 22 75 2 55 1.51e-12 57.0
MS.gene45670.t1 AT4G26650 41.176 68 38 1 7 74 13 78 1.62e-12 61.2
MS.gene45670.t1 AT3G07810 48.000 50 26 0 7 56 7 56 2.16e-12 60.8
MS.gene45670.t1 AT5G53680 41.935 62 36 0 7 68 14 75 2.18e-12 58.9
MS.gene45670.t1 AT5G40490 39.706 68 41 0 7 74 114 181 2.22e-12 60.8
MS.gene45670.t1 AT3G07810 48.000 50 26 0 7 56 7 56 2.22e-12 60.8
MS.gene45670.t1 AT1G01080 37.681 69 42 1 1 69 207 274 2.29e-12 60.5
MS.gene45670.t1 AT4G36960 41.667 72 39 1 4 75 1 69 2.68e-12 60.5
MS.gene45670.t1 AT4G36960 41.667 72 39 1 4 75 1 69 2.68e-12 60.5
MS.gene45670.t1 AT3G52380 34.722 72 47 0 3 74 216 287 2.68e-12 60.5
MS.gene45670.t1 AT1G17640 45.763 59 32 0 7 65 67 125 2.81e-12 60.5
MS.gene45670.t1 AT1G17640 49.091 55 28 0 7 61 158 212 5.07e-12 59.7
MS.gene45670.t1 AT1G17640 45.763 59 32 0 7 65 67 125 2.81e-12 60.5
MS.gene45670.t1 AT1G17640 49.091 55 28 0 7 61 158 212 5.07e-12 59.7
MS.gene45670.t1 AT1G17640 45.763 59 32 0 7 65 67 125 2.81e-12 60.5
MS.gene45670.t1 AT1G17640 49.091 55 28 0 7 61 158 212 5.07e-12 59.7
MS.gene45670.t1 AT5G47620 48.000 50 26 0 7 56 7 56 2.88e-12 60.5
MS.gene45670.t1 AT5G47620 48.000 50 26 0 7 56 7 56 3.72e-12 60.1
MS.gene45670.t1 AT5G47620 48.000 50 26 0 7 56 7 56 3.72e-12 60.1
MS.gene45670.t1 AT5G47620 48.000 50 26 0 7 56 7 56 3.72e-12 60.1
MS.gene45670.t1 AT3G52150 39.726 73 44 0 3 75 174 246 4.33e-12 59.3
MS.gene45670.t1 AT3G52150 39.726 73 44 0 3 75 174 246 4.33e-12 59.3
MS.gene45670.t1 AT5G40490 47.619 63 33 0 3 65 39 101 4.84e-12 59.7
MS.gene45670.t1 AT5G40490 39.706 68 41 0 7 74 131 198 1.79e-11 58.2
MS.gene45670.t1 AT2G37510 34.737 95 37 1 7 76 35 129 6.44e-12 57.8
MS.gene45670.t1 AT1G54080 41.538 65 38 0 6 70 148 212 6.49e-12 59.3
MS.gene45670.t1 AT4G14300 38.462 65 40 0 7 71 7 71 7.19e-12 59.3
MS.gene45670.t1 AT4G14300 38.462 65 40 0 7 71 7 71 7.19e-12 59.3
MS.gene45670.t1 AT3G14100 40.000 65 39 0 6 70 144 208 8.05e-12 59.3
MS.gene45670.t1 AT1G58470 46.154 52 28 0 6 57 6 57 1.32e-11 58.5
MS.gene45670.t1 AT1G58470 46.154 52 28 0 6 57 6 57 1.32e-11 58.5
MS.gene45670.t1 AT2G22100 43.860 57 32 0 9 65 166 222 1.61e-11 58.2
MS.gene45670.t1 AT2G33410 35.593 59 38 0 7 65 7 65 2.99e-11 57.4
MS.gene45670.t1 AT4G34110 41.538 65 37 1 9 73 218 281 3.24e-11 57.4
MS.gene45670.t1 AT3G20930 35.294 68 44 0 7 74 283 350 4.28e-11 57.0
MS.gene45670.t1 AT4G27000 43.284 67 37 1 5 70 172 238 4.53e-11 57.0
MS.gene45670.t1 AT2G41060 41.935 62 36 0 4 65 126 187 5.23e-11 57.0
MS.gene45670.t1 AT2G41060 41.935 62 36 0 4 65 126 187 5.23e-11 57.0
MS.gene45670.t1 AT2G41060 41.935 62 36 0 4 65 126 187 5.23e-11 57.0
MS.gene45670.t1 AT3G06970 38.983 59 36 0 7 65 13 71 6.10e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT3G56860 38.710 62 38 0 4 65 138 199 7.11e-11 56.6
MS.gene45670.t1 AT1G73530 31.944 72 49 0 5 76 76 147 8.17e-11 55.1

Find 20 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGACGATCAAGAGGTTT+TGG 0.283486 4.4:-37787781 MS.gene45670:CDS
ATCATTAACGATCGTGAAAC+TGG 0.301664 4.4:-37787799 MS.gene45670:CDS
AGCAATGAACGGTCAGGATT+TGG 0.315035 4.4:-37787713 MS.gene45670:CDS
TGCTTCAATGGCATCGTTCA+TGG 0.319166 4.4:+37787732 None:intergenic
CTGACCGTTCATTGCTTCAA+TGG 0.378604 4.4:+37787720 None:intergenic
TGATTGTTATTCTAGATGTT+GGG 0.408580 4.4:-37787390 MS.gene45670:intron
GCTTCGTCGGAGGTCTTGCA+TGG 0.475042 4.4:-37788217 MS.gene45670:CDS
CATTGAAGCAATGAACGGTC+AGG 0.516837 4.4:-37787719 MS.gene45670:CDS
CAAGACCTCCGACGAAGCAT+CGG 0.524569 4.4:+37788222 None:intergenic
GTTGAATACCGATGCTTCGT+CGG 0.525860 4.4:-37788230 MS.gene45670:CDS
CGGCGAGATCGTTGATTCCA+AGG 0.539852 4.4:-37788153 MS.gene45670:intron
GAGAAAGCCTTCTCTCAATA+CGG 0.543855 4.4:-37788173 MS.gene45670:CDS
TTTCTCGAGAGCATCGTTGT+CGG 0.548634 4.4:+37788190 None:intergenic
CGTGAAACTGGACGATCAAG+AGG 0.551662 4.4:-37787787 MS.gene45670:CDS
CTCGAGAGCATCGTTGTCGG+TGG 0.585909 4.4:+37788193 None:intergenic
GAATACCGATGCTTCGTCGG+AGG 0.590495 4.4:-37788227 MS.gene45670:CDS
CTTCGTCGGAGGTCTTGCAT+GGG 0.591814 4.4:-37788216 MS.gene45670:CDS
GATCTCGCCGTATTGAGAGA+AGG 0.650761 4.4:+37788166 None:intergenic
GATGCCATTGAAGCAATGAA+CGG 0.670960 4.4:-37787724 MS.gene45670:CDS
ATGAACGGTCAGGATTTGGA+CGG 0.698077 4.4:-37787709 MS.gene45670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCTTTTGATATTTA+GGG - chr4.4:37787765-37787784 MS.gene45670:CDS 10.0%
!!! TTTTTTTTCTTTTGATATTT+AGG - chr4.4:37787764-37787783 MS.gene45670:CDS 10.0%
!! ATGATTGTTATTCTAGATGT+TGG - chr4.4:37788232-37788251 MS.gene45670:CDS 25.0%
!! TGATTGTTATTCTAGATGTT+GGG - chr4.4:37788233-37788252 MS.gene45670:CDS 25.0%
AACAAAAGTTGAAGATCGAT+CGG - chr4.4:37787708-37787727 MS.gene45670:CDS 30.0%
!!! GATTTTGATTCGGATTTCTA+TGG - chr4.4:37787532-37787551 MS.gene45670:intron 30.0%
!!! TTTAGGGTTTTATTCTGATC+TGG - chr4.4:37787781-37787800 MS.gene45670:CDS 30.0%
AAGAAGATGAACGTACCTAT+TGG + chr4.4:37787682-37787701 None:intergenic 35.0%
AAGATCGATCGGTTACTATT+CGG - chr4.4:37787719-37787738 MS.gene45670:CDS 35.0%
ATCATTAACGATCGTGAAAC+TGG - chr4.4:37787824-37787843 MS.gene45670:intron 35.0%
TATTACTCACTCACACCAAT+AGG - chr4.4:37787664-37787683 MS.gene45670:intron 35.0%
!! ACTATTCGGTTCTAGATCTA+TGG - chr4.4:37787733-37787752 MS.gene45670:CDS 35.0%
!!! GATCTGACACGATTTTGATT+CGG - chr4.4:37787522-37787541 MS.gene45670:intron 35.0%
CAGAGTAACACAGAACAGAT+CGG + chr4.4:37787580-37787599 None:intergenic 40.0%
GAGAAAGCCTTCTCTCAATA+CGG - chr4.4:37787450-37787469 MS.gene45670:intron 40.0%
GATGCCATTGAAGCAATGAA+CGG - chr4.4:37787899-37787918 MS.gene45670:intron 40.0%
AGCAATGAACGGTCAGGATT+TGG - chr4.4:37787910-37787929 MS.gene45670:intron 45.0%
ATGAACGGTCAGGATTTGGA+CGG - chr4.4:37787914-37787933 MS.gene45670:intron 45.0%
CATTGAAGCAATGAACGGTC+AGG - chr4.4:37787904-37787923 MS.gene45670:intron 45.0%
CTGACCGTTCATTGCTTCAA+TGG + chr4.4:37787906-37787925 None:intergenic 45.0%
GTTGAATACCGATGCTTCGT+CGG - chr4.4:37787393-37787412 MS.gene45670:intron 45.0%
TCGGAGATCACAGATCAGAA+AGG + chr4.4:37787561-37787580 None:intergenic 45.0%
TGCTTCAATGGCATCGTTCA+TGG + chr4.4:37787894-37787913 None:intergenic 45.0%
! ACTGGACGATCAAGAGGTTT+TGG - chr4.4:37787842-37787861 MS.gene45670:intron 45.0%
!! AGGAGGTGATGGAGGTTATT+AGG - chr4.4:37788186-37788205 MS.gene45670:CDS 45.0%
!! TTTCTCGAGAGCATCGTTGT+CGG + chr4.4:37787436-37787455 None:intergenic 45.0%
CGAAGCCTGCAAGCAACAAA+CGG + chr4.4:37787497-37787516 None:intergenic 50.0%
CGTGAAACTGGACGATCAAG+AGG - chr4.4:37787836-37787855 MS.gene45670:intron 50.0%
GATCTCGCCGTATTGAGAGA+AGG + chr4.4:37787460-37787479 None:intergenic 50.0%
TACAACAGAGGAGGAGGTGA+TGG - chr4.4:37788175-37788194 MS.gene45670:CDS 50.0%
TGCAAGCAACAAACGGACCT+TGG + chr4.4:37787490-37787509 None:intergenic 50.0%
! AAGGTCCGTTTGTTGCTTGC+AGG - chr4.4:37787489-37787508 MS.gene45670:intron 50.0%
! CGTTTGTTGCTTGCAGGCTT+CGG - chr4.4:37787495-37787514 MS.gene45670:intron 50.0%
CAAGACCTCCGACGAAGCAT+CGG + chr4.4:37787404-37787423 None:intergenic 55.0%
CTTCGTCGGAGGTCTTGCAT+GGG - chr4.4:37787407-37787426 MS.gene45670:intron 55.0%
GAATACCGATGCTTCGTCGG+AGG - chr4.4:37787396-37787415 MS.gene45670:intron 55.0%
GGTGGAGAACGTCGTGGATA+CGG - chr4.4:37788034-37788053 MS.gene45670:intron 55.0%
GGTGGATACAACAGAGGAGG+AGG - chr4.4:37788169-37788188 MS.gene45670:CDS 55.0%
GTTGCTTGCAGGCTTCGGAT+CGG - chr4.4:37787500-37787519 MS.gene45670:intron 55.0%
! AACAGAGGAGGAGGTGATGG+AGG - chr4.4:37788178-37788197 MS.gene45670:CDS 55.0%
! GGATATGGTGGAGAACGTCG+TGG - chr4.4:37788028-37788047 MS.gene45670:intron 55.0%
!! AGTGGTGGATATGGTGGAGG+AGG - chr4.4:37788064-37788083 MS.gene45670:intron 55.0%
!! CGGCGAGATCGTTGATTCCA+AGG - chr4.4:37787470-37787489 MS.gene45670:intron 55.0%
CAAGCTCAGAGCCGTGGTAG+CGG - chr4.4:37787956-37787975 MS.gene45670:intron 60.0%
CTCCATAACCACCGCCACCA+CGG + chr4.4:37787997-37788016 None:intergenic 60.0%
GAACGTCGTGGATACGGTGG+AGG - chr4.4:37788040-37788059 MS.gene45670:intron 60.0%
GCTTCGTCGGAGGTCTTGCA+TGG - chr4.4:37787406-37787425 MS.gene45670:intron 60.0%
GGAGAACGTCGTGGATACGG+TGG - chr4.4:37788037-37788056 MS.gene45670:intron 60.0%
GGCGGTGGATACAACAGAGG+AGG - chr4.4:37788166-37788185 MS.gene45670:CDS 60.0%
GGTGGTTACAGCAGAGGAGG+CGG - chr4.4:37788127-37788146 MS.gene45670:intron 60.0%
GTCAACCAAGCTCAGAGCCG+TGG - chr4.4:37787950-37787969 MS.gene45670:intron 60.0%
TGTAACCACCGCCTCCACCA+CGG + chr4.4:37788117-37788136 None:intergenic 60.0%
! GGATATGGTGGAGGAGGTGG+TGG - chr4.4:37788070-37788089 MS.gene45670:intron 60.0%
! GGTGGATATGGTGGAGGAGG+TGG - chr4.4:37788067-37788086 MS.gene45670:intron 60.0%
! GGTGGCGGTGGATACAACAG+AGG - chr4.4:37788163-37788182 MS.gene45670:CDS 60.0%
! GGTGGTTATGGAGGTGGTGG+CGG - chr4.4:37788004-37788023 MS.gene45670:intron 60.0%
!! CTCGAGAGCATCGTTGTCGG+TGG + chr4.4:37787433-37787452 None:intergenic 60.0%
!! GGAGGAGGTGGTGGATATGG+CGG - chr4.4:37788079-37788098 MS.gene45670:intron 60.0%
!! GGAGGCAGTGGTGGATATGG+TGG - chr4.4:37788058-37788077 MS.gene45670:intron 60.0%
!! GGCAGTGGTGGATATGGTGG+AGG - chr4.4:37788061-37788080 MS.gene45670:intron 60.0%
!! GGTGGAGGAGGTGGTGGATA+TGG - chr4.4:37788076-37788095 MS.gene45670:intron 60.0%
!! GGTGGAGGCAGTGGTGGATA+TGG - chr4.4:37788055-37788074 MS.gene45670:intron 60.0%
!! GGTGGTGGTGGATATGGTGG+CGG - chr4.4:37788148-37788167 MS.gene45670:intron 60.0%
CGTGGATACGGTGGAGGCAG+TGG - chr4.4:37788046-37788065 MS.gene45670:intron 65.0%
CTCCTCCACCACCGCTACCA+CGG + chr4.4:37787970-37787989 None:intergenic 65.0%
GGAGGCGGTGGTTACAGCAG+AGG - chr4.4:37788121-37788140 MS.gene45670:intron 65.0%
GGATACGGTGGAGGCAGTGG+TGG - chr4.4:37788049-37788068 MS.gene45670:intron 65.0%
GGCGGTGGTTACAGCAGAGG+AGG - chr4.4:37788124-37788143 MS.gene45670:intron 65.0%
GGCGGTGGTTATGGAGGTGG+TGG - chr4.4:37788001-37788020 MS.gene45670:intron 65.0%
GGTTACAGCAGAGGAGGCGG+TGG - chr4.4:37788130-37788149 MS.gene45670:intron 65.0%
TACAGCAGAGGAGGCGGTGG+TGG - chr4.4:37788133-37788152 MS.gene45670:intron 65.0%
! GGTGGCGGTGGTTATGGAGG+TGG - chr4.4:37787998-37788017 MS.gene45670:intron 65.0%
!! CGCTACCACGGCTCTGAGCT+TGG + chr4.4:37787958-37787977 None:intergenic 65.0%
!! CGTGGTGGCGGTGGTTATGG+AGG - chr4.4:37787995-37788014 MS.gene45670:intron 65.0%
!! GGAGGCGGTGGTGGTGGATA+TGG - chr4.4:37788142-37788161 MS.gene45670:intron 65.0%
!! GGAGGTGGTGGATATGGCGG+TGG - chr4.4:37788082-37788101 MS.gene45670:intron 65.0%
!! GGAGGTGGTGGCGGTGGATA+TGG - chr4.4:37788013-37788032 MS.gene45670:intron 65.0%
!! GGCGGTGGTGGTGGATATGG+TGG - chr4.4:37788145-37788164 MS.gene45670:intron 65.0%
!! GGTGGTGGATATGGCGGTGG+TGG - chr4.4:37788085-37788104 MS.gene45670:intron 65.0%
!! GGTGGTGGATATGGTGGCGG+TGG - chr4.4:37788151-37788170 MS.gene45670:intron 65.0%
!! GGTGGTGGCGGTGGATATGG+TGG - chr4.4:37788016-37788035 MS.gene45670:intron 65.0%
!! GGTTATGGAGGTGGTGGCGG+TGG - chr4.4:37788007-37788026 MS.gene45670:intron 65.0%
!! GTGGTAGCGGTGGTGGAGGA+GGG - chr4.4:37787969-37787988 MS.gene45670:intron 65.0%
!! TGGTAGCGGTGGTGGAGGAG+GGG - chr4.4:37787970-37787989 MS.gene45670:intron 65.0%
GGTGGTGATCGCCGTGGTGG+AGG - chr4.4:37788103-37788122 MS.gene45670:intron 70.0%
! AGCAGAGGAGGCGGTGGTGG+TGG - chr4.4:37788136-37788155 MS.gene45670:intron 70.0%
! GGTGATCGCCGTGGTGGAGG+CGG - chr4.4:37788106-37788125 MS.gene45670:intron 70.0%
! GGTGGTGGTGATCGCCGTGG+TGG - chr4.4:37788100-37788119 MS.gene45670:intron 70.0%
!! AGCCGTGGTAGCGGTGGTGG+AGG - chr4.4:37787965-37787984 MS.gene45670:intron 70.0%
!! CAGAGCCGTGGTAGCGGTGG+TGG - chr4.4:37787962-37787981 MS.gene45670:intron 70.0%
!! CGTGGTAGCGGTGGTGGAGG+AGG - chr4.4:37787968-37787987 MS.gene45670:intron 70.0%
!! GCTCAGAGCCGTGGTAGCGG+TGG - chr4.4:37787959-37787978 MS.gene45670:intron 70.0%
!! GGCCGTGGTGGCGGTGGTTA+TGG - chr4.4:37787992-37788011 MS.gene45670:intron 70.0%
!! GGTAGCGGTGGTGGAGGAGG+GGG - chr4.4:37787971-37787990 MS.gene45670:intron 70.0%
! GATCGCCGTGGTGGAGGCGG+TGG - chr4.4:37788109-37788128 MS.gene45670:intron 75.0%
! GGCGGTGGTGGTGATCGCCG+TGG - chr4.4:37788097-37788116 MS.gene45670:intron 75.0%
! GGAGGAGGGGGCCGTGGTGG+CGG - chr4.4:37787983-37788002 MS.gene45670:intron 80.0%
! GGTGGAGGAGGGGGCCGTGG+TGG - chr4.4:37787980-37787999 MS.gene45670:intron 80.0%
!!! GGTGGTGGAGGAGGGGGCCG+TGG - chr4.4:37787977-37787996 MS.gene45670:intron 80.0%
!!! GGAGGGGGCCGTGGTGGCGG+TGG - chr4.4:37787986-37788005 MS.gene45670:intron 85.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 37787384 37788261 37787384 ID=MS.gene45670
chr4.4 mRNA 37787384 37788261 37787384 ID=MS.gene45670.t1;Parent=MS.gene45670
chr4.4 exon 37788154 37788261 37788154 ID=MS.gene45670.t1.exon1;Parent=MS.gene45670.t1
chr4.4 CDS 37788154 37788261 37788154 ID=cds.MS.gene45670.t1;Parent=MS.gene45670.t1
chr4.4 exon 37787707 37787821 37787707 ID=MS.gene45670.t1.exon2;Parent=MS.gene45670.t1
chr4.4 CDS 37787707 37787821 37787707 ID=cds.MS.gene45670.t1;Parent=MS.gene45670.t1
chr4.4 exon 37787384 37787397 37787384 ID=MS.gene45670.t1.exon3;Parent=MS.gene45670.t1
chr4.4 CDS 37787384 37787397 37787384 ID=cds.MS.gene45670.t1;Parent=MS.gene45670.t1
Gene Sequence

>MS.gene45670

ATGGCTGATGTTGAATACCGATGCTTCGTCGGAGGTCTTGCATGGGCCACCGACAACGATGCTCTCGAGAAAGCCTTCTCTCAATACGGCGAGATCGTTGATTCCAAGGTCCGTTTGTTGCTTGCAGGCTTCGGATCGGATCTGACACGATTTTGATTCGGATTTCTATGGAAACCTTTCTGATCTGTGATCTCCGATCTGTTCTGTGTTACTCTGTTACTTGATCTATTTGTTACTGTTACTCTGATTCTGATTCTGTTACTGTATGATTTGATTCTTTTATTACTCACTCACACCAATAGGTACGTTCATCTTCTTCTTTTGAACAAAAGTTGAAGATCGATCGGTTACTATTCGGTTCTAGATCTATGGATCTGATTTTTTTTTTCTTTTGATATTTAGGGTTTTATTCTGATCTGGTTTGTTAATTTTTTGTGTAGATCATTAACGATCGTGAAACTGGACGATCAAGAGGTTTTGGATTCGTGACATTCGCTAACGAGAAATCCATGAACGATGCCATTGAAGCAATGAACGGTCAGGATTTGGACGGACGTAACATCACTGTCAACCAAGCTCAGAGCCGTGGTAGCGGTGGTGGAGGAGGGGGCCGTGGTGGCGGTGGTTATGGAGGTGGTGGCGGTGGATATGGTGGAGAACGTCGTGGATACGGTGGAGGCAGTGGTGGATATGGTGGAGGAGGTGGTGGATATGGCGGTGGTGGTGATCGCCGTGGTGGAGGCGGTGGTTACAGCAGAGGAGGCGGTGGTGGTGGATATGGTGGCGGTGGATACAACAGAGGAGGAGGTGATGGAGGTTATTAGGTTTAGATTAAATGTTTATCGTTTATGATTGTTATTCTAGATGTTGGGATCTAG
Protein sequence

>MS.gene45670.t1

MADVEYRCFVGGLAWATDNDALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIEAMNGQDLDGHVGI