Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45689.t1 | RHN61275.1 | 82.9 | 105 | 18 | 0 | 1 | 105 | 34 | 138 | 2.40E-38 | 169.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45689.t1 | G7JG40 | 82.9 | 105 | 18 | 0 | 1 | 105 | 34 | 138 | 1.8e-38 | 169.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene45689 | 0.820422 | 6.92E-53 | -1.69E-46 |
| MS.gene050338 | MS.gene45689 | 0.800644 | 1.31E-48 | -1.69E-46 |
| MS.gene052702 | MS.gene45689 | 0.804611 | 1.99E-49 | -1.69E-46 |
| MS.gene054884 | MS.gene45689 | 0.811851 | 5.69E-51 | -1.69E-46 |
| MS.gene056694 | MS.gene45689 | 0.829236 | 5.76E-55 | -1.69E-46 |
| MS.gene056697 | MS.gene45689 | 0.868683 | 5.44E-66 | -1.69E-46 |
| MS.gene05926 | MS.gene45689 | 0.825059 | 5.76E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45689.t1 | MTR_4g069810 | 65.758 | 330 | 13 | 3 | 1 | 230 | 34 | 363 | 3.32e-135 | 385 |
| MS.gene45689.t1 | MTR_4g069810 | 58.503 | 147 | 56 | 3 | 51 | 196 | 237 | 379 | 1.34e-45 | 156 |
| MS.gene45689.t1 | MTR_3g116430 | 32.971 | 276 | 128 | 13 | 1 | 224 | 33 | 303 | 1.02e-28 | 112 |
| MS.gene45689.t1 | MTR_3g116430 | 36.957 | 184 | 106 | 7 | 44 | 220 | 225 | 405 | 5.89e-24 | 99.4 |
| MS.gene45689.t1 | MTR_3g116430 | 39.796 | 98 | 57 | 2 | 44 | 140 | 330 | 426 | 5.64e-13 | 67.8 |
| MS.gene45689.t1 | MTR_3g034610 | 35.769 | 260 | 121 | 13 | 1 | 224 | 1 | 250 | 1.80e-28 | 111 |
| MS.gene45689.t1 | MTR_3g034610 | 37.500 | 184 | 104 | 6 | 44 | 220 | 198 | 377 | 1.37e-27 | 108 |
| MS.gene45689.t1 | MTR_3g034610 | 37.234 | 188 | 105 | 8 | 44 | 223 | 172 | 354 | 4.54e-27 | 107 |
| MS.gene45689.t1 | MTR_3g034610 | 38.251 | 183 | 106 | 6 | 44 | 221 | 224 | 404 | 2.76e-26 | 105 |
| MS.gene45689.t1 | MTR_3g116440 | 35.714 | 238 | 128 | 9 | 1 | 220 | 33 | 263 | 7.56e-28 | 108 |
| MS.gene45689.t1 | MTR_3g116440 | 35.176 | 199 | 109 | 4 | 44 | 222 | 161 | 359 | 8.65e-23 | 95.1 |
| MS.gene45689.t1 | MTR_3g034640 | 38.191 | 199 | 103 | 11 | 44 | 229 | 207 | 398 | 1.35e-27 | 109 |
| MS.gene45689.t1 | MTR_3g034640 | 37.705 | 183 | 105 | 7 | 44 | 220 | 259 | 438 | 8.81e-26 | 104 |
| MS.gene45689.t1 | MTR_3g034640 | 37.569 | 181 | 108 | 5 | 44 | 220 | 311 | 490 | 1.40e-25 | 104 |
| MS.gene45689.t1 | MTR_3g034640 | 37.363 | 182 | 107 | 6 | 44 | 220 | 285 | 464 | 1.65e-24 | 101 |
| MS.gene45689.t1 | MTR_3g034640 | 35.178 | 253 | 128 | 10 | 1 | 220 | 33 | 282 | 3.28e-24 | 100 |
| MS.gene45689.t1 | MTR_3g034640 | 36.154 | 130 | 80 | 3 | 44 | 171 | 363 | 491 | 1.65e-16 | 78.2 |
| MS.gene45689.t1 | MTR_3g107810 | 31.687 | 243 | 140 | 9 | 1 | 220 | 33 | 272 | 1.69e-15 | 75.1 |
| MS.gene45689.t1 | MTR_3g107810 | 32.432 | 185 | 116 | 7 | 42 | 222 | 299 | 478 | 1.11e-12 | 67.0 |
| MS.gene45689.t1 | MTR_3g107810 | 29.717 | 212 | 108 | 8 | 44 | 220 | 249 | 454 | 1.45e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACCTTGTCGCACTTGGTC+TGG | 0.282329 | 4.4:+37970270 | None:intergenic |
| ATACTTTGTAGCACTTGGTC+TGG | 0.301950 | 4.4:+37970420 | None:intergenic |
| AGACCCACAATTTCAGCTTA+TGG | 0.346620 | 4.4:-37970652 | MS.gene45689:CDS |
| AGAAGCCCTTGAATTCCTTC+TGG | 0.355426 | 4.4:+37970814 | None:intergenic |
| ATACTTTGTAACACTTGGTC+TGG | 0.359796 | 4.4:+37970195 | None:intergenic |
| TACCTTGTCGCACTTGGTCT+GGG | 0.372376 | 4.4:+37970271 | None:intergenic |
| GGCAAGTTGCTCTTGTGCCT+TGG | 0.386448 | 4.4:+37970756 | None:intergenic |
| ATAACTTGTAACACTTGGTA+TGG | 0.408404 | 4.4:+37970345 | None:intergenic |
| ATGCTCATCGCACTTACCTT+TGG | 0.413648 | 4.4:+37970735 | None:intergenic |
| ATATTTAGTGTGTTTGTTGC+GGG | 0.414691 | 4.4:+37970712 | None:intergenic |
| ATACCAAGTGTTACAAGTTA+TGG | 0.431717 | 4.4:-37970343 | MS.gene45689:CDS |
| GTTATAAAAGCGAGAAGCTA+TGG | 0.433941 | 4.4:-37970543 | MS.gene45689:CDS |
| TACTTTGTAGCACTTGGTCT+GGG | 0.436190 | 4.4:+37970421 | None:intergenic |
| CCATCATACTTTGTAACACT+TGG | 0.443820 | 4.4:+37970190 | None:intergenic |
| CCATCATACTTTGTAGCACT+TGG | 0.452221 | 4.4:+37970496 | None:intergenic |
| CCATCATACTTTGTAGCACT+TGG | 0.452826 | 4.4:+37970415 | None:intergenic |
| GGTTCAAACTCGTCATTAAC+CGG | 0.460302 | 4.4:+37970292 | None:intergenic |
| TACCTTGTGACACTTGGCCT+TGG | 0.460410 | 4.4:+37970577 | None:intergenic |
| CCGCTATACCTTGTGACACT+TGG | 0.461174 | 4.4:+37970571 | None:intergenic |
| GGTTCAAACTCGTCATTCCT+TGG | 0.473350 | 4.4:+37970442 | None:intergenic |
| TGTGCCTTGGAGTTCTTCAA+AGG | 0.482951 | 4.4:+37970769 | None:intergenic |
| TAAGCTGAAATTGTGGGTCT+TGG | 0.493615 | 4.4:+37970655 | None:intergenic |
| GGTTCAAATCCGTCGTTCAT+CGG | 0.495800 | 4.4:+37970217 | None:intergenic |
| CTGCCATAACTTGTAACACT+TGG | 0.506622 | 4.4:+37970340 | None:intergenic |
| GAGATGCCAGAAGGAATTCA+AGG | 0.507467 | 4.4:-37970820 | MS.gene45689:CDS |
| CCGTCATACCTTGTCGCACT+TGG | 0.513672 | 4.4:+37970265 | None:intergenic |
| GATATTTAGTGTGTTTGTTG+CGG | 0.527545 | 4.4:+37970711 | None:intergenic |
| TCACCATAAGCTGAAATTGT+GGG | 0.533189 | 4.4:+37970649 | None:intergenic |
| TACTTTGTAACACTTGGTCT+GGG | 0.542995 | 4.4:+37970196 | None:intergenic |
| AAGTATGATGGCGATGGTGA+AGG | 0.556090 | 4.4:-37970484 | MS.gene45689:CDS |
| CCAAGTGCTACAAAGTATGA+TGG | 0.558846 | 4.4:-37970496 | MS.gene45689:CDS |
| GTTACAAGTTATGGCAGTGA+TGG | 0.561348 | 4.4:-37970334 | MS.gene45689:CDS |
| CCAAGTGTTACAAAGTATGA+TGG | 0.562453 | 4.4:-37970190 | MS.gene45689:CDS |
| GTCACAAGGTATAGCGGTGA+TGG | 0.565412 | 4.4:-37970565 | MS.gene45689:CDS |
| TACTTTGTAGCACTTGGCCT+AGG | 0.571553 | 4.4:+37970502 | None:intergenic |
| TAACTTGTAACACTTGGTAT+GGG | 0.572877 | 4.4:+37970346 | None:intergenic |
| CCAAGTGCTACAAAGTATGA+TGG | 0.583943 | 4.4:-37970415 | MS.gene45689:CDS |
| ATCACCATAAGCTGAAATTG+TGG | 0.595027 | 4.4:+37970648 | None:intergenic |
| CTATAAGAGTATGAAACTAC+CGG | 0.601733 | 4.4:-37970311 | MS.gene45689:CDS |
| TTATAAAAGCGAGAAGCTAT+GGG | 0.613278 | 4.4:-37970542 | MS.gene45689:CDS |
| AAAGATGAAGAGATGCCAGA+AGG | 0.616352 | 4.4:-37970829 | MS.gene45689:CDS |
| AGCCAAGGCCAAGTGTCACA+AGG | 0.624209 | 4.4:-37970579 | MS.gene45689:CDS |
| TTATCAGAGTACAAAACTAT+CGG | 0.624493 | 4.4:-37970386 | MS.gene45689:CDS |
| ATGAAACTACCGATGAACGA+CGG | 0.632128 | 4.4:-37970226 | MS.gene45689:CDS |
| GCACAAGAGCAACTTGCCAA+AGG | 0.634334 | 4.4:-37970751 | MS.gene45689:CDS |
| CCAAGTGCGACAAGGTATGA+CGG | 0.634896 | 4.4:-37970265 | MS.gene45689:CDS |
| TGTCCTCTGAGTTTGAACCT+AGG | 0.640039 | 4.4:-37970519 | MS.gene45689:CDS |
| AGATGCCAGAAGGAATTCAA+GGG | 0.644893 | 4.4:-37970819 | MS.gene45689:CDS |
| AACCCAGACCAAGTGCGACA+AGG | 0.648941 | 4.4:-37970273 | MS.gene45689:CDS |
| CCAAGTGTCACAAGGTATAG+CGG | 0.650077 | 4.4:-37970571 | MS.gene45689:CDS |
| ATATGACTATTAAACTACCA+AGG | 0.661952 | 4.4:-37970459 | MS.gene45689:CDS |
| GCTACAAAGTATGATGGTGA+TGG | 0.662559 | 4.4:-37970409 | MS.gene45689:CDS |
| CGAACCTTTGAAGAACTCCA+AGG | 0.682187 | 4.4:-37970773 | MS.gene45689:CDS |
| GCTACAAAGTATGATGGCGA+TGG | 0.687351 | 4.4:-37970490 | MS.gene45689:CDS |
| TGGCCTAGGTTCAAACTCAG+AGG | 0.693294 | 4.4:+37970516 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAAATATCATAGAAAAAA+AGG | - | chr4.4:37970293-37970312 | MS.gene45689:CDS | 15.0% |
| !! | AAATATCATAGAAAAAAAGG+TGG | - | chr4.4:37970296-37970315 | MS.gene45689:CDS | 20.0% |
| ! | ATATGACTATTAAACTACCA+AGG | - | chr4.4:37970532-37970551 | MS.gene45689:CDS | 25.0% |
| ! | TTATCAGAGTACAAAACTAT+CGG | - | chr4.4:37970605-37970624 | MS.gene45689:CDS | 25.0% |
| !! | ATATAAAAGATTTTGAGCCA+AGG | - | chr4.4:37970397-37970416 | MS.gene45689:CDS | 25.0% |
| ATACCAAGTGTTACAAGTTA+TGG | - | chr4.4:37970648-37970667 | MS.gene45689:CDS | 30.0% | |
| TTATAAAAGCGAGAAGCTAT+GGG | - | chr4.4:37970449-37970468 | MS.gene45689:CDS | 30.0% | |
| ! | ATAACTTGTAACACTTGGTA+TGG | + | chr4.4:37970649-37970668 | None:intergenic | 30.0% |
| ! | ATATTTAGTGTGTTTGTTGC+GGG | + | chr4.4:37970282-37970301 | None:intergenic | 30.0% |
| ! | CTATAAGAGTATGAAACTAC+CGG | - | chr4.4:37970680-37970699 | MS.gene45689:CDS | 30.0% |
| ! | GATATTTAGTGTGTTTGTTG+CGG | + | chr4.4:37970283-37970302 | None:intergenic | 30.0% |
| ! | TAACTTGTAACACTTGGTAT+GGG | + | chr4.4:37970648-37970667 | None:intergenic | 30.0% |
| ATACTTTGTAACACTTGGTC+TGG | + | chr4.4:37970799-37970818 | None:intergenic | 35.0% | |
| ATCACCATAAGCTGAAATTG+TGG | + | chr4.4:37970346-37970365 | None:intergenic | 35.0% | |
| CCAAGTGTTACAAAGTATGA+TGG | - | chr4.4:37970801-37970820 | MS.gene45689:CDS | 35.0% | |
| CCATCATACTTTGTAACACT+TGG | + | chr4.4:37970804-37970823 | None:intergenic | 35.0% | |
| GTTATAAAAGCGAGAAGCTA+TGG | - | chr4.4:37970448-37970467 | MS.gene45689:CDS | 35.0% | |
| TACTTTGTAACACTTGGTCT+GGG | + | chr4.4:37970798-37970817 | None:intergenic | 35.0% | |
| TCACCATAAGCTGAAATTGT+GGG | + | chr4.4:37970345-37970364 | None:intergenic | 35.0% | |
| AAAGATGAAGAGATGCCAGA+AGG | - | chr4.4:37970162-37970181 | MS.gene45689:CDS | 40.0% | |
| AGACCCACAATTTCAGCTTA+TGG | - | chr4.4:37970339-37970358 | MS.gene45689:CDS | 40.0% | |
| AGATGCCAGAAGGAATTCAA+GGG | - | chr4.4:37970172-37970191 | MS.gene45689:CDS | 40.0% | |
| ATACTTTGTAGCACTTGGTC+TGG | + | chr4.4:37970574-37970593 | None:intergenic | 40.0% | |
| ATGAAACTACCGATGAACGA+CGG | - | chr4.4:37970765-37970784 | MS.gene45689:CDS | 40.0% | |
| CCAAGTGCTACAAAGTATGA+TGG | - | chr4.4:37970495-37970514 | MS.gene45689:CDS | 40.0% | |
| CCATCATACTTTGTAGCACT+TGG | + | chr4.4:37970498-37970517 | None:intergenic | 40.0% | |
| GGTTCAAACTCGTCATTAAC+CGG | + | chr4.4:37970702-37970721 | None:intergenic | 40.0% | |
| GTTACAAGTTATGGCAGTGA+TGG | - | chr4.4:37970657-37970676 | MS.gene45689:CDS | 40.0% | |
| TAAGCTGAAATTGTGGGTCT+TGG | + | chr4.4:37970339-37970358 | None:intergenic | 40.0% | |
| TACTTTGTAGCACTTGGTCT+GGG | + | chr4.4:37970573-37970592 | None:intergenic | 40.0% | |
| ! | CTGCCATAACTTGTAACACT+TGG | + | chr4.4:37970654-37970673 | None:intergenic | 40.0% |
| ! | GCTACAAAGTATGATGGTGA+TGG | - | chr4.4:37970582-37970601 | MS.gene45689:CDS | 40.0% |
| AGAAGCCCTTGAATTCCTTC+TGG | + | chr4.4:37970180-37970199 | None:intergenic | 45.0% | |
| ATGCTCATCGCACTTACCTT+TGG | + | chr4.4:37970259-37970278 | None:intergenic | 45.0% | |
| CCAAGTGTCACAAGGTATAG+CGG | - | chr4.4:37970420-37970439 | MS.gene45689:CDS | 45.0% | |
| GAGATGCCAGAAGGAATTCA+AGG | - | chr4.4:37970171-37970190 | MS.gene45689:CDS | 45.0% | |
| GGTTCAAACTCGTCATTCCT+TGG | + | chr4.4:37970552-37970571 | None:intergenic | 45.0% | |
| GGTTCAAATCCGTCGTTCAT+CGG | + | chr4.4:37970777-37970796 | None:intergenic | 45.0% | |
| TACTTTGTAGCACTTGGCCT+AGG | + | chr4.4:37970492-37970511 | None:intergenic | 45.0% | |
| TGTCCTCTGAGTTTGAACCT+AGG | - | chr4.4:37970472-37970491 | MS.gene45689:CDS | 45.0% | |
| TGTGCCTTGGAGTTCTTCAA+AGG | + | chr4.4:37970225-37970244 | None:intergenic | 45.0% | |
| ! | AAGTATGATGGCGATGGTGA+AGG | - | chr4.4:37970507-37970526 | MS.gene45689:CDS | 45.0% |
| ! | CGAACCTTTGAAGAACTCCA+AGG | - | chr4.4:37970218-37970237 | MS.gene45689:CDS | 45.0% |
| ! | GCTACAAAGTATGATGGCGA+TGG | - | chr4.4:37970501-37970520 | MS.gene45689:CDS | 45.0% |
| ATACCTTGTCGCACTTGGTC+TGG | + | chr4.4:37970724-37970743 | None:intergenic | 50.0% | |
| CCAAGTGCGACAAGGTATGA+CGG | - | chr4.4:37970726-37970745 | MS.gene45689:CDS | 50.0% | |
| CCGCTATACCTTGTGACACT+TGG | + | chr4.4:37970423-37970442 | None:intergenic | 50.0% | |
| GCACAAGAGCAACTTGCCAA+AGG | - | chr4.4:37970240-37970259 | MS.gene45689:CDS | 50.0% | |
| GTCACAAGGTATAGCGGTGA+TGG | - | chr4.4:37970426-37970445 | MS.gene45689:CDS | 50.0% | |
| TACCTTGTCGCACTTGGTCT+GGG | + | chr4.4:37970723-37970742 | None:intergenic | 50.0% | |
| TACCTTGTGACACTTGGCCT+TGG | + | chr4.4:37970417-37970436 | None:intergenic | 50.0% | |
| TGGCCTAGGTTCAAACTCAG+AGG | + | chr4.4:37970478-37970497 | None:intergenic | 50.0% | |
| AACCCAGACCAAGTGCGACA+AGG | - | chr4.4:37970718-37970737 | MS.gene45689:CDS | 55.0% | |
| AGCCAAGGCCAAGTGTCACA+AGG | - | chr4.4:37970412-37970431 | MS.gene45689:CDS | 55.0% | |
| CCGTCATACCTTGTCGCACT+TGG | + | chr4.4:37970729-37970748 | None:intergenic | 55.0% | |
| GGCAAGTTGCTCTTGTGCCT+TGG | + | chr4.4:37970238-37970257 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 37970159 | 37970854 | 37970159 | ID=MS.gene45689 |
| chr4.4 | mRNA | 37970159 | 37970854 | 37970159 | ID=MS.gene45689.t1;Parent=MS.gene45689 |
| chr4.4 | exon | 37970159 | 37970854 | 37970159 | ID=MS.gene45689.t1.exon1;Parent=MS.gene45689.t1 |
| chr4.4 | CDS | 37970159 | 37970854 | 37970159 | ID=cds.MS.gene45689.t1;Parent=MS.gene45689.t1 |
| Gene Sequence |
| Protein sequence |
