Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45692.t1 | XP_003606879.2 | 91.7 | 96 | 6 | 1 | 1 | 96 | 11 | 104 | 1.60E-39 | 171.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45692.t1 | G7JG36 | 91.7 | 96 | 6 | 1 | 1 | 96 | 1 | 94 | 1.1e-39 | 171.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05087 | MS.gene45692 | -0.812523 | 4.06E-51 | -1.69E-46 |
| MS.gene056693 | MS.gene45692 | 0.962892 | 2.02E-121 | -1.69E-46 |
| MS.gene059802 | MS.gene45692 | 0.834194 | 3.45E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45692.t1 | MTR_4g069770 | 91.667 | 96 | 6 | 1 | 1 | 96 | 1 | 94 | 1.40e-60 | 179 |
| MS.gene45692.t1 | MTR_5g095980 | 66.667 | 78 | 20 | 1 | 1 | 72 | 1 | 78 | 1.48e-25 | 91.7 |
| MS.gene45692.t1 | MTR_3g007880 | 61.538 | 78 | 24 | 1 | 1 | 72 | 1 | 78 | 2.77e-22 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45692.t1 | AT1G49310 | 42.254 | 71 | 37 | 2 | 8 | 74 | 12 | 82 | 6.70e-13 | 58.9 |
Find 11 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTCTTCTTCTGCTTCT+TGG | 0.166429 | 4.4:+38032323 | None:intergenic |
| GAAAAGACTTGGGAGATTAT+TGG | 0.261061 | 4.4:-38032479 | MS.gene45692:CDS |
| ACAAGTTCTTTGATTGCTTC+AGG | 0.324229 | 4.4:+38032429 | None:intergenic |
| AACTCTGAACAAGAGCTTCA+TGG | 0.354626 | 4.4:-38032285 | MS.gene45692:CDS |
| CTCTTATGCAAGAAAAGACT+TGG | 0.442343 | 4.4:-38032490 | MS.gene45692:CDS |
| GAGTATGAGAGAAGCAAAGA+TGG | 0.451907 | 4.4:+38032675 | None:intergenic |
| TCTTTGATTGCTTCAGGCAT+AGG | 0.472850 | 4.4:+38032435 | None:intergenic |
| GTGTGATATAATATGACATT+AGG | 0.480243 | 4.4:+38032357 | None:intergenic |
| TGATTGCTTCAGGCATAGGT+TGG | 0.514568 | 4.4:+38032439 | None:intergenic |
| AGAACTTGTTCAAAATCAAG+AGG | 0.539159 | 4.4:-38032415 | MS.gene45692:CDS |
| TCTTATGCAAGAAAAGACTT+GGG | 0.611202 | 4.4:-38032489 | MS.gene45692:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATATAGTATAAATTGAT+TGG | + | chr4.4:38032405-38032424 | None:intergenic | 15.0% |
| !!! | AATTTTCTTTGTACTAAAAT+AGG | - | chr4.4:38032437-38032456 | MS.gene45692:CDS | 15.0% |
| !! | TTAGTACAAAGAAAATTGAA+AGG | + | chr4.4:38032434-38032453 | None:intergenic | 20.0% |
| ! | GTGTGATATAATATGACATT+AGG | + | chr4.4:38032606-38032625 | None:intergenic | 25.0% |
| TCTTATGCAAGAAAAGACTT+GGG | - | chr4.4:38032471-38032490 | MS.gene45692:CDS | 30.0% | |
| ! | AGAACTTGTTCAAAATCAAG+AGG | - | chr4.4:38032545-38032564 | MS.gene45692:intron | 30.0% |
| ACAAGTTCTTTGATTGCTTC+AGG | + | chr4.4:38032534-38032553 | None:intergenic | 35.0% | |
| CTCTTATGCAAGAAAAGACT+TGG | - | chr4.4:38032470-38032489 | MS.gene45692:CDS | 35.0% | |
| GAAAAGACTTGGGAGATTAT+TGG | - | chr4.4:38032481-38032500 | MS.gene45692:CDS | 35.0% | |
| ! | AGTCTTTTCTTGCATAAGAG+AGG | + | chr4.4:38032470-38032489 | None:intergenic | 35.0% |
| ! | CATACTCTTTTCTCTTCTTC+TGG | - | chr4.4:38032301-38032320 | MS.gene45692:CDS | 35.0% |
| ! | TTGTTTCTTCTTCTGCTTCT+TGG | + | chr4.4:38032640-38032659 | None:intergenic | 35.0% |
| AACTCTGAACAAGAGCTTCA+TGG | - | chr4.4:38032675-38032694 | MS.gene45692:CDS | 40.0% | |
| GAGTATGAGAGAAGCAAAGA+TGG | + | chr4.4:38032288-38032307 | None:intergenic | 40.0% | |
| TCTTTGATTGCTTCAGGCAT+AGG | + | chr4.4:38032528-38032547 | None:intergenic | 40.0% | |
| TGATTGCTTCAGGCATAGGT+TGG | + | chr4.4:38032524-38032543 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 38032278 | 38032704 | 38032278 | ID=MS.gene45692 |
| chr4.4 | mRNA | 38032278 | 38032704 | 38032278 | ID=MS.gene45692.t1;Parent=MS.gene45692 |
| chr4.4 | exon | 38032660 | 38032704 | 38032660 | ID=MS.gene45692.t1.exon1;Parent=MS.gene45692.t1 |
| chr4.4 | CDS | 38032660 | 38032704 | 38032660 | ID=cds.MS.gene45692.t1;Parent=MS.gene45692.t1 |
| chr4.4 | exon | 38032278 | 38032523 | 38032278 | ID=MS.gene45692.t1.exon2;Parent=MS.gene45692.t1 |
| chr4.4 | CDS | 38032278 | 38032523 | 38032278 | ID=cds.MS.gene45692.t1;Parent=MS.gene45692.t1 |
| Gene Sequence |
| Protein sequence |
